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https://www.readbyqxmd.com/read/29216900/treatment-with-a-dna-methyltransferase-inhibitor-feminizes-zebrafish-and-induces-long-term-expression-changes-in-the-gonads
#1
Laia Ribas, Konstantinos Vanezis, Marco Antonio Imués, Francesc Piferrer
BACKGROUND: The role of epigenetic modifications such as DNA methylation during vertebrate sexual development is far from being clear. Using the zebrafish model, we tested the effects of one of the most common DNA methyltransferase (dnmt) inhibitor, 5-aza-2'-deoxycytidine (5-aza-dC), which is approved for the treatment of acute myeloid leukaemia and is under active investigation for the treatment of solid tumours. Several dose-response experiments were carried out during two periods, including not only the very first days of development (0-6 days post-fertilization, dpf), as done in previous studies, but also, and as a novelty, the period of gonadal development (10-30 dpf)...
December 8, 2017: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/29073214/robust-gdf9-and-bmp15-expression-in-the-oocytes-of-ovotestes-through-the-figla-independent-pathway-in-the-hermaphroditic-black-porgy-acanthopagrus-schlegelii
#2
Guan-Chung Wu, Jia-Wun Luo, Hau-Wen Li, Chen-Hsiu Huang, Ching-Fong Chang
More than 1,500 fish species are hermaphroditic, but no hermaphroditic lineage appears to be evolutionarily ancient in fishes. Thus, whether more than one sex at a time was present during the evolutionary shift from gonochorism to hermaphroditism in fishes is an intriguing question. Ectopic oocytes were created in the ovotestes of protandrous black porgy via the withdrawal of estradiol (E2) administration. These ectopic oocytes reprogrammed the surrounding cells, which changed from Sertoli cells to follicle-like cells...
2017: PloS One
https://www.readbyqxmd.com/read/28826812/gonadal-development-and-expression-of-sex-specific-genes-during-sex-differentiation-in-the-japanese-eel
#3
Shan-Ru Jeng, Guan-Chung Wu, Wen-Shiun Yueh, Shu-Fen Kuo, Sylvie Dufour, Ching-Fong Chang
The process of gonadal development and mechanism involved in sex differentiation in eels are still unclear. The objectives were to investigate the gonadal development and expression pattern of sex-related genes during sex differentiation in the Japanese eel, Anguilla japonica. For control group, the elvers of 8-10 cm were reared for 8 months; and for feminization, estradiol-17β (E2) was orally administered to the elvers of 8-10 cm for 6 months. Only males were found in the control group, suggesting a possible role of environmental factors in eel sex determination...
August 4, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28738802/histological-and-transcriptomic-effects-of-17%C3%AE-methyltestosterone-on-zebrafish-gonad-development
#4
Stephanie Ling Jie Lee, Julia A Horsfield, Michael A Black, Kim Rutherford, Amanda Fisher, Neil J Gemmell
BACKGROUND: Sex hormones play important roles in teleost ovarian and testicular development. In zebrafish, ovarian differentiation appears to be dictated by an oocyte-derived signal via Cyp19a1a aromatase-mediated estrogen production. Androgens and aromatase inhibitors can induce female-to-male sex reversal, however, the mechanisms underlying gonadal masculinisation are poorly understood. We used histological analyses together with RNA sequencing to characterise zebrafish gonadal transcriptomes and investigate the effects of 17α-methyltestosterone on gonadal differentiation...
July 24, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28663108/expression-patterns-of-sex-differentiation-related-genes-during-gonadal-sex-change-in-the-protogynous-wrasse-halichoeres-trimaculatus
#5
Ryo Horiguchi, Ryo Nozu, Toshiaki Hirai, Yasuhisa Kobayashi, Masaru Nakamura
The three-spot wrasse, Halichoeres trimaculatus, can change sex from female to male (i.e. protogyny) due to sharp decrease in endogenous estrogen. During the sex change, ovarian tissue degenerates and testicular tissue arises newly. Finally, ovarian tissue disappears completely and replaces into mature testis. In order to predict the molecular mechanisms controlling the processes of sex change, we investigated the expression patterns of four genes (rspo1, figla, sox9b and amh), which have been thought to be associated with ovarian/testicular differentiation in vertebrates...
June 26, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28489928/identification-and-analysis-of-the-%C3%AE-catenin1-gene-in-half-smooth-tongue-sole-cynoglossus-semilaevis
#6
Ying Zhu, Qiaomu Hu, Wenteng Xu, Hailong Li, Hua Guo, Liang Meng, Min Wei, Sheng Lu, Changwei Shao, Na Wang, Guanpin Yang, Songlin Chen
β-catenin is a key signalling molecule in the canonical Wnt pathway, which plays a role in cell adhesion, embryogenesis and sex determination. However, little is known about its function in teleosts. We cloned and characterized the full-length β-catenin1 gene from half-smooth tongue sole (Cynoglossus semilaevis), which was designated CS-β-catenin1. The CS-β-catenin1 cDNA consists of 2,346 nucleotides and encodes a protein with 782 amino acids. Although CS-β-catenin1 was transcribed in the gonads of both sexes, the level was significantly higher in ovaries compared to testes...
2017: PloS One
https://www.readbyqxmd.com/read/28489605/genetic-rearrangements-result-in-altered-gene-expression-and-novel-fusion-transcripts-in-s%C3%A3-zary-syndrome
#7
Katarzyna Iżykowska, Grzegorz K Przybylski, Claudia Gand, Floriane C Braun, Piotr Grabarczyk, Andreas W Kuss, Karolina Olek-Hrab, Armando N Bastidas Torres, Maarten H Vermeer, Willem H Zoutman, Cornelis P Tensen, Christian A Schmidt
Sézary syndrome (SS) is an aggressive, leukemic cutaneous T-cell lymphoma variant. Molecular pathogenesis of SS is still unclear despite many studies on genetic alterations, gene expression and epigenetic regulations. Through whole genome and transcriptome next generation sequencing nine Sézary syndrome patients were analyzed in terms of copy number variations and rearrangements affecting gene expression. Recurrent copy number variations were detected within 8q (MYC, TOX), 17p (TP53, NCOR1), 10q (PTEN, FAS), 2p (DNMT3A), 11q (USP28), 9p (CAAP1), but no recurrent rearrangements were identified...
June 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/28377895/short-term-culture-of-vitrified-human-ovarian-cortical-tissue-to-assess-the-cryopreservation-outcome-molecular-and-morphological-analysis
#8
Mehdi Ramezani, Mojdeh Salehnia, Mina Jafarabadi
BACKGROUND: The aim of the present study was to evaluate the effectiveness of human ovarian vitrification protocol followed with in vitro culture at the morphological and molecular levels. METHODS: Ovarian tissues were obtained from 10 normal transsexual women and cut into small pieces and were divided into non-vitrified and vitrified groups and some of the tissues fragments in both groups were randomly cultured for two weeks. The morphological study using hematoxylin and eosin and Masson's trichrome staining was done...
January 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/28367413/expression-of-folliculogenesis-related-genes-in-vitrified-human-ovarian-tissue-after-two-weeks-in-vitro-culture
#9
Zahra Shams Mofarahe, Mojdeh Salehnia, Marefat Ghaffari Novin, Nassim Ghorbanmehr, Mohammad Gholami Fesharaki
OBJECTIVE: This study was designed to evaluate the effects of vitrification and in vitro culture of human ovarian tissue on the expression of oocytic and follicular cell-related genes. MATERIALS AND METHODS: In this experimental study, ovarian tissue samples were obtained from eight transsexual women. Samples were cut into small fragments and were then assigned to vitrified and non-vitrified groups. In each group, some tissue fragments were divided into un-cultured and cultured (in α-MEM medium for 2 weeks) subgroups...
April 2017: Cell Journal
https://www.readbyqxmd.com/read/28289071/disorganization-of-the-germ-cell-pool-leads-to-primary-ovarian-insufficiency
#10
Ikko Kawashima, Kazuhiro Kawamura
The mammalian ovary is an organ that controls female germ cell development, storing them and releasing mature oocytes for transporting to the oviduct. During the fetal stage, female germ cells change from a proliferative state to meiosis before forming follicles with the potential for growth of surrounding somatic cells. Understanding of molecular and physiological bases of germ cell development in the fetal ovary contributed not only to the elucidation of genetic disorders in primary ovarian insufficiency (POI), but also advancement of novel treatments for patients with POI...
March 13, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28199921/pilot-scale-bioconversion-of-rice-and-sunflower-agro-residues-into-medicinal-mushrooms-and-laccase-enzymes-through-solid-state-fermentation-with-ganoderma-lucidum
#11
P D Postemsky, M A Bidegain, R González-Matute, N D Figlas, M A Cubitto
Solid-state fermentation was evaluated at the pilot-scale for the bioconversion and valorization of rice husks and straw (RSH), or sunflower seed hulls (SSH), into medicinal mushrooms and crude extracts, with laccase activity. The average mushroom yield was 56kg dry weight per ton of agro-residues. Laccase activity in crude aqueous extracts showed its maximum value of 10,927Ukg(-1) in RSH (day 10, Exudate phase) and 16,442Ukg(-1) in SSH (day 5, Full colonization phase), the activity at the Residual substrate phase being 511Ukg(-1) in RSH and 803Ukg(-1) in SSH, respectively...
May 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27893173/bmp15l-figla-smc1bl-and-larp6l-are-preferentially-expressed-in-germ-cells-in-atlantic-salmon-salmo-salar-l
#12
Lene Kleppe, Rolf Brudvik Edvardsen, Tomasz Furmanek, Eva Andersson, Amélie Juanchich, Anna Wargelius
Atlantic salmon is a valuable commercial aquaculture species that would benefit economically and environmentally by controlling precocious puberty and preventing escapees from reproducing with wild populations. One solution to both these challenges is the production of sterile individuals by inhibiting the formation of germ cells, but achieving this requires more information on the specific factors that control germ cell formation. Here, we identified and characterized novel factors that are preferentially expressed in Atlantic salmon germ cells by screening for gonad-specific genes using available adult multi-tissue transcriptomes...
November 7, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27716808/bovine-lhx8-a-germ-cell-specific-nuclear-factor-interacts-with-figla
#13
Liyuan Fu, Mingxiang Zhang, Kristen Mastrantoni, Mark Perfetto, Shuo Wei, Jianbo Yao
LIM homeobox 8 (Lhx8) is a germ cell-specific transcription factor essential for the development of oocytes during early oogenesis. In mice, Lhx8 deficiency causes postnatal oocyte loss and affects the expression of many oocyte-specific genes. The aims of this study were to characterize the bovine Lhx8 gene, determine its mRNA expression during oocyte development and early embryogenesis, and evaluate its interactions with other oocyte-specific transcription factors. The bovine Lhx8 gene encodes a protein of 377 amino acids...
2016: PloS One
https://www.readbyqxmd.com/read/27603904/identification-of-multiple-gene-mutations-accounts-for-a-new-genetic-architecture-of-primary-ovarian-insufficiency
#14
Justine Bouilly, Isabelle Beau, Sara Barraud, Valérie Bernard, Kemal Azibi, Jérôme Fagart, Anne Fèvre, Anne Laure Todeschini, Reiner A Veitia, Chérif Beldjord, Brigitte Delemer, Catherine Dodé, Jacques Young, Nadine Binart
CONTEXT: Idiopathic primary ovarian insufficiency (POI) is a major cause of amenorrhea and infertility. POI affects 1% of women before age 40 years, and several genetic causes have been reported. To date, POI has been considered a monogenic disorder. OBJECTIVE: The aim of this study was to identify novel gene variations and to investigate if individuals with POI harbor mutation in multiple loci. PATIENTS AND METHODS: One hundred well-phenotyped POI patients were systematically screened for variants in 19 known POI loci (and potential candidate genes) using next-generation sequencing...
December 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27341508/taf4b-regulates-oocyte-specific-genes-essential-for-meiosis
#15
Kathryn J Grive, Eric A Gustafson, Kimberly A Seymour, Melody Baddoo, Christoph Schorl, Kayla Golnoski, Aleksandar Rajkovic, Alexander S Brodsky, Richard N Freiman
TAF4b is a gonadal-enriched subunit of the general transcription factor TFIID that is implicated in promoting healthy ovarian aging and female fertility in mice and humans. To further explore the potential mechanism of TAF4b in promoting ovarian follicle development, we analyzed global gene expression at multiple time points in the human fetal ovary. This computational analysis revealed coordinate expression of human TAF4B and critical regulators and effectors of meiosis I including SYCP3, YBX2, STAG3, and DAZL...
June 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27313147/two-figla-homologues-have-disparate-functions-during-sex-differentiation-in-half-smooth-tongue-sole-cynoglossus-semilaevis
#16
Hailong Li, Wenteng Xu, Ning Zhang, Changwei Shao, Ying Zhu, Zhongdian Dong, Na Wang, Xiaodong Jia, Hao Xu, Songlin Chen
Figla is a germ-cell-specific transcription factor associated with ovary development and differentiation. In vertebrates, one transcriptional form of Figla is commonly found. However, besides the common form of this gene (named Figla_tv1), a new transcriptional form (named Figla_tv2) was identified in half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of Figla_tv1 was 1057 bp long with a 591-bp open reading frame encoding a predicted 196 amino acid protein, while Figla_tv2 encoded a 125 amino acid protein...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26714271/sexual-fate-reprogramming-in-the-steroid-induced-bi-directional-sex-change-in-the-protogynous-orange-spotted-grouper-epinephelus-coioides
#17
Guan-Chung Wu, Wei-Guan Tey, Hau-Wen Li, Ching-Fong Chang
Androgen administration has been widely used for masculinization in fish. The mechanism of the sex change in sexual fate regulation is not clear. Oral administration or pellet implantation was applied. We orally applied an aromatase inhibitor (AI, to decrease estrogen levels) and 17α-methyltestosterone (MT, to increase androgen levels) to induce masculinization to clarify the mechanism of the sex change in the protogynous orange-spotted grouper. After 3 mo of AI/MT administration, male characteristics were observed in the female-to-male sex change fish...
2015: PloS One
https://www.readbyqxmd.com/read/26243799/genetics-of-primary-ovarian-insufficiency-new-developments-and-opportunities
#18
REVIEW
Yingying Qin, Xue Jiao, Joe Leigh Simpson, Zi-Jiang Chen
BACKGROUND: Primary ovarian insufficiency (POI) is characterized by marked heterogeneity, but with a significant genetic contribution. Identifying exact causative genes has been challenging, with many discoveries not replicated. It is timely to take stock of the field, outlining the progress made, framing the controversies and anticipating future directions in elucidating the genetics of POI. METHODS: A search for original articles published up to May 2015 was performed using PubMed and Google Scholar, identifying studies on the genetic etiology of POI...
November 2015: Human Reproduction Update
https://www.readbyqxmd.com/read/26175108/effect-of-human-ovarian-tissue-vitrification-warming-on-the-expression-of-genes-related-to-folliculogenesis
#19
Zahra Shams Mofarahe, Marefat Ghaffari Novin, Mina Jafarabadi, Mojdeh Salehnia, Mohsen Noroozian, Nassim Ghorbanmehr
BACKGROUND: Ovarian tissue cryopreservation is an alternative strategy to preserve the fertility of women predicted to undergo premature ovarian failure. This study was designed to evaluate the expression of folliculogenesis-related genes, including factor in the germline alpha (FIGLA), growth differentiation factor-9 (GDF-9), follicle-stimulating hormone receptor (FSHR), and KIT LIGAND after vitrification/warming of human ovarian tissue. METHODS: Human ovarian tissue samples were collected from five transsexual women...
2015: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/26040669/lats1-deletion-causes-increased-germ-cell-apoptosis-and-follicular-cysts-in-mouse-ovaries
#20
Tianyanxin Sun, Melissa E Pepling, Francisco J Diaz
The Hippo signaling pathway is essential for regulating proliferation and apoptosis in mammalian cells. The LATS1 kinase is a core member of the Hippo signaling pathway that phosphorylates and inactivates the transcriptional co-activators YAP1 and WWTR1. Deletion of Lats1 results in low neonate survival and ovarian stromal tumors in surviving adults, but the effects of Lats1 on early follicular development are not understood. Here, the expression of Hippo pathway components including Wwtr1, Stk4, Stk3, Lats2, and Yap1 transcripts were decreased by 50% in mouse ovaries between 2 and 8 days of age while expression was maintained from 8 days to 21 days and after priming with eCG...
July 2015: Biology of Reproduction
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