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Mehdi Ramezani, Mojdeh Salehnia, Mina Jafarabadi
BACKGROUND: The aim of the present study was to evaluate the effectiveness of human ovarian vitrification protocol followed with in vitro culture at the morphological and molecular levels. METHODS: Ovarian tissues were obtained from 10 normal transsexual women and cut into small pieces and were divided into non-vitrified and vitrified groups and some of the tissues fragments in both groups were randomly cultured for two weeks. The morphological study using hematoxylin and eosin and Masson's trichrome staining was done...
January 2017: Journal of Reproduction & Infertility
Zahra Shams Mofarahe, Mojdeh Salehnia, Marefat Ghaffari Novin, Nassim Ghorbanmehr, Mohammad Gholami Fesharaki
OBJECTIVE: This study was designed to evaluate the effects of vitrification and in vitro culture of human ovarian tissue on the expression of oocytic and follicular cell-related genes. MATERIALS AND METHODS: In this experimental study, ovarian tissue samples were obtained from eight transsexual women. Samples were cut into small fragments and were then assigned to vitrified and non-vitrified groups. In each group, some tissue fragments were divided into un-cultured and cultured (in α-MEM medium for 2 weeks) subgroups...
April 2017: Cell Journal
Ikko Kawashima, Kazuhiro Kawamura
The mammalian ovary is an organ that controls female germ cell development, storing them and releasing mature oocytes for transporting to the oviduct. During the fetal stage, female germ cells change from a proliferative state to meiosis before forming follicles with the potential for growth of surrounding somatic cells. Understanding of molecular and physiological bases of germ cell development in the fetal ovary contributed not only to the elucidation of genetic disorders in primary ovarian insufficiency (POI), but also advancement of novel treatments for patients with POI...
March 13, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
P D Postemsky, M A Bidegain, R González-Matute, N D Figlas, M A Cubitto
Solid-state fermentation was evaluated at the pilot-scale for the bioconversion and valorization of rice husks and straw (RSH), or sunflower seed hulls (SSH), into medicinal mushrooms and crude extracts, with laccase activity. The average mushroom yield was 56kg dry weight per ton of agro-residues. Laccase activity in crude aqueous extracts showed its maximum value of 10,927Ukg(-1) in RSH (day 10, Exudate phase) and 16,442Ukg(-1) in SSH (day 5, Full colonization phase), the activity at the Residual substrate phase being 511Ukg(-1) in RSH and 803Ukg(-1) in SSH, respectively...
May 2017: Bioresource Technology
Lene Kleppe, Rolf Brudvik Edvardsen, Tomasz Furmanek, Eva Andersson, Amélie Juanchich, Anna Wargelius
Atlantic salmon is a valuable commercial aquaculture species that would benefit economically and environmentally by controlling precocious puberty and preventing escapees from reproducing with wild populations. One solution to both these challenges is the production of sterile individuals by inhibiting the formation of germ cells, but achieving this requires more information on the specific factors that control germ cell formation. Here, we identified and characterized novel factors that are preferentially expressed in Atlantic salmon germ cells by screening for gonad-specific genes using available adult multi-tissue transcriptomes...
November 7, 2016: Molecular Reproduction and Development
Liyuan Fu, Mingxiang Zhang, Kristen Mastrantoni, Mark Perfetto, Shuo Wei, Jianbo Yao
LIM homeobox 8 (Lhx8) is a germ cell-specific transcription factor essential for the development of oocytes during early oogenesis. In mice, Lhx8 deficiency causes postnatal oocyte loss and affects the expression of many oocyte-specific genes. The aims of this study were to characterize the bovine Lhx8 gene, determine its mRNA expression during oocyte development and early embryogenesis, and evaluate its interactions with other oocyte-specific transcription factors. The bovine Lhx8 gene encodes a protein of 377 amino acids...
2016: PloS One
Justine Bouilly, Isabelle Beau, Sara Barraud, Valérie Bernard, Kemal Azibi, Jérôme Fagart, Anne Fèvre, Anne Laure Todeschini, Reiner A Veitia, Chérif Beldjord, Brigitte Delemer, Catherine Dodé, Jacques Young, Nadine Binart
CONTEXT: Idiopathic primary ovarian insufficiency (POI) is a major cause of amenorrhea and infertility. POI affects 1% of women before age 40 years, and several genetic causes have been reported. To date, POI has been considered a monogenic disorder. OBJECTIVE: The aim of this study was to identify novel gene variations and to investigate if individuals with POI harbor mutation in multiple loci. PATIENTS AND METHODS: One hundred well-phenotyped POI patients were systematically screened for variants in 19 known POI loci (and potential candidate genes) using next-generation sequencing...
December 2016: Journal of Clinical Endocrinology and Metabolism
Kathryn J Grive, Eric A Gustafson, Kimberly A Seymour, Melody Baddoo, Christoph Schorl, Kayla Golnoski, Aleksandar Rajkovic, Alexander S Brodsky, Richard N Freiman
TAF4b is a gonadal-enriched subunit of the general transcription factor TFIID that is implicated in promoting healthy ovarian aging and female fertility in mice and humans. To further explore the potential mechanism of TAF4b in promoting ovarian follicle development, we analyzed global gene expression at multiple time points in the human fetal ovary. This computational analysis revealed coordinate expression of human TAF4B and critical regulators and effectors of meiosis I including SYCP3, YBX2, STAG3, and DAZL...
June 2016: PLoS Genetics
Hailong Li, Wenteng Xu, Ning Zhang, Changwei Shao, Ying Zhu, Zhongdian Dong, Na Wang, Xiaodong Jia, Hao Xu, Songlin Chen
Figla is a germ-cell-specific transcription factor associated with ovary development and differentiation. In vertebrates, one transcriptional form of Figla is commonly found. However, besides the common form of this gene (named Figla_tv1), a new transcriptional form (named Figla_tv2) was identified in half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of Figla_tv1 was 1057 bp long with a 591-bp open reading frame encoding a predicted 196 amino acid protein, while Figla_tv2 encoded a 125 amino acid protein...
2016: Scientific Reports
Guan-Chung Wu, Wei-Guan Tey, Hau-Wen Li, Ching-Fong Chang
Androgen administration has been widely used for masculinization in fish. The mechanism of the sex change in sexual fate regulation is not clear. Oral administration or pellet implantation was applied. We orally applied an aromatase inhibitor (AI, to decrease estrogen levels) and 17α-methyltestosterone (MT, to increase androgen levels) to induce masculinization to clarify the mechanism of the sex change in the protogynous orange-spotted grouper. After 3 mo of AI/MT administration, male characteristics were observed in the female-to-male sex change fish...
2015: PloS One
Yingying Qin, Xue Jiao, Joe Leigh Simpson, Zi-Jiang Chen
BACKGROUND: Primary ovarian insufficiency (POI) is characterized by marked heterogeneity, but with a significant genetic contribution. Identifying exact causative genes has been challenging, with many discoveries not replicated. It is timely to take stock of the field, outlining the progress made, framing the controversies and anticipating future directions in elucidating the genetics of POI. METHODS: A search for original articles published up to May 2015 was performed using PubMed and Google Scholar, identifying studies on the genetic etiology of POI...
November 2015: Human Reproduction Update
Zahra Shams Mofarahe, Marefat Ghaffari Novin, Mina Jafarabadi, Mojdeh Salehnia, Mohsen Noroozian, Nassim Ghorbanmehr
BACKGROUND: Ovarian tissue cryopreservation is an alternative strategy to preserve the fertility of women predicted to undergo premature ovarian failure. This study was designed to evaluate the expression of folliculogenesis-related genes, including factor in the germline alpha (FIGLA), growth differentiation factor-9 (GDF-9), follicle-stimulating hormone receptor (FSHR), and KIT LIGAND after vitrification/warming of human ovarian tissue. METHODS: Human ovarian tissue samples were collected from five transsexual women...
2015: Iranian Biomedical Journal
Tianyanxin Sun, Melissa E Pepling, Francisco J Diaz
The Hippo signaling pathway is essential for regulating proliferation and apoptosis in mammalian cells. The LATS1 kinase is a core member of the Hippo signaling pathway that phosphorylates and inactivates the transcriptional co-activators YAP1 and WWTR1. Deletion of Lats1 results in low neonate survival and ovarian stromal tumors in surviving adults, but the effects of Lats1 on early follicular development are not understood. Here, the expression of Hippo pathway components including Wwtr1, Stk4, Stk3, Lats2, and Yap1 transcripts were decreased by 50% in mouse ovaries between 2 and 8 days of age while expression was maintained from 8 days to 21 days and after priming with eCG...
July 2015: Biology of Reproduction
Yongxiu Qiu, Shaohua Sun, Tapas Charkraborty, Limin Wu, Lina Sun, Jing Wei, Yoshitaka Nagahama, Deshou Wang, Linyan Zhou
Figla (factor in the germ line, alpha), a female germ cell-specific transcription factor, had been shown to activate genetic hierarchies in oocytes. The ectopic expression of Figla was known to repress spermatogenesis-associated genes in male mice. However, the potential role of Figla in other vertebrates remains elusive. The present work was aimed to identify and characterize the functional relevance of Figla in the ovarian development of Nile tilapia (Oreochromis niloticus). Tissue distribution and ontogeny analysis revealed that tilapia Figla gene was dominantly expressed in the ovary from 30 days after hatching...
2015: PloS One
Min-Woo Park, Kyeoung-Hwa Kim, Eun-Young Kim, Su-Yeon Lee, Jung-Jae Ko, Kyung-Ah Lee
In a previous report, we identified Sebox as a new candidate maternal effect gene that is essential for embryonic development and primarily impacts the two-cell (2C) stage. The present study was conducted to determine the mechanism of action for Sebox in this capacity, as shown by changes in the expression levels of other known MEG mRNAs after Sebox RNA interference (RNAi) in oocytes. Sebox-knockdown metaphase II (Mll) oocytes displayed normal morphology, but among the 23 MEGs monitored, 8 genes were upregulated, and 15 genes were unchanged...
2015: PloS One
Zhili Chu, Bowen Niu, Na Li, Yue Hu, Jian Li, Ping Yu, Chongyang Wu, Xinrong Yan, Anmin Lei, Jinlian Hua
Premature ovarian failure (POF) is affecting more and more women, which is the loss of function of the ovaries before age 40. To elucidate the underlying mechanisms of the oogenesis is of importance to understand the causes of impaired fertility and POF. However, mammalian oogenesis in vivo is a complex process. Thus, building an oogenesis visualizing system is beneficial for the study of oogenesis. In this study, we found that Figla is specifically expressed in female mice oocyte. Then, we constructed a lentiviral vector (pTRIP-Figla-EGFP-puro) under the control of Figla promoter, which drived enhanced green fluorescent protein (EGFP) as an indicator and used the lentiviral vector transduction the ovarian cells and induced germ cells derived from human umbilical cord mesenchymal stem cells (hUC-MSCs), and the results showed that the lentiviral vector we constructed was able to specifically express green fluorescent protein (GFP) in the ovarian oocyte and induced oocyte-like cells derived from hUC-MSCs, which was Figla-positive cells...
May 2015: Applied Biochemistry and Biotechnology
Durgadatta Tosh, Hanumanth Surekha Rani, Upadhyayula Suryanarayana Murty, Anupama Deenadayal, Paramjit Grover
OBJECTIVE: The aim of our study was to identify the association of FIGLA (factor in the germ line α) gene variants with premature ovarian failure (POF) in the Indian population. METHODS: Two hundred nineteen women with idiopathic POF and 230 healthy controls were recruited for this study. All exons, intron-exon boundaries, and untranslated regions of the FIGLA gene were analyzed by polymerase chain reaction and sequencing. All FIGLA variants were analyzed in silico, using PolyPhen, SIFT, MutationTaster, PMUT, I-Mutant, Mupro, Align-GVGD, PROVEAN, and HOPE software, to predict pathogenicity and changes in protein stability...
May 2015: Menopause: the Journal of the North American Menopause Society
Linlin Pan, Wei Gong, Yuanyuan Zhou, Xiaonuan Li, Jun Yu, Songnian Hu
Ovary development is a complex process involving numerous genes. A well-developed ovary is essential for females to keep fertility and reproduce offspring. In order to gain a better insight into the molecular mechanisms related to the process of mammalian ovary development, we performed a comparative transcriptomic analysis on ovaries isolated from infant and adult mice by using next-generation sequencing technology (SOLiD). We identified 15,454 and 16,646 transcriptionally active genes at the infant and adult stage, respectively...
October 2014: Genomics, Proteomics & Bioinformatics
R González Matute, D Figlas, N Curvetto
Agaricus blazei Murrill is usually cultivated using the same biphasic composting method employed for A. bisporus. Because cultivation of A. blazei on traditional A. bisporus composts poses some disadvantages, non-composted substrates were studied for A. blazei cultivation. Mycelial growth rate and productive performance of A. blazei were evaluated on substrates containing sunflower seed hulls, Pleurotus spp. spent mushroom substrate, or their combination, in the absence or in the presence of different supplements (vermicompost, peat or brewery residues)...
June 2011: World Journal of Microbiology & Biotechnology
Cong Yuan, Tingting Wu, Yingying Zhang, Jiancao Gao, Yanping Yang, Fang Qin, Shaozhen Liu, Yao Zheng, Zaizhao Wang
Proteins encoded by figla, foxl2, scp3 and sox9a play important roles in gonad differentiation and reproduction. In the present study, we aimed to determine the responsiveness of figla, foxl2, scp3 and sox9a to 17α-ethinylestradiol (EE2) in the gonads of adult Gobiocypris rarus. Full-length cDNAs of figla, scp3 and sox9a were cloned and characterized by RT-PCR and RACE methods. Expression patterns in adult tissues were investigated. Results indicated that figla was predominantly expressed in adult ovaries and scp3 was restrictively expressed in the male testes and sox9a was principally expressed in the brains of both genders and the testes of males...
May 1, 2014: General and Comparative Endocrinology
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