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Burkholderia cenocepacia

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https://www.readbyqxmd.com/read/29363827/a-novel-two-component-system-modulates-quorum-sensing-and-pathogenicity-in-burkholderia-cenocepacia
#1
Chaoyu Cui, Chunxi Yang, Shihao Song, Shuna Fu, Xiuyun Sun, Liang Yang, Fei He, Lian-Hui Zhang, Yongliang Zhang, Yinyue Deng
Quorum sensing (QS) is widely utilized by bacterial pathogens to regulate biological functions and pathogenicity. Recent evidence has shown that QS is subject to regulatory cascades, especially two-component systems that often respond to environmental stimulation. At least two different types of QS systems regulate pathogenesis in Burkholderia cenocepacia. However, it remains unclear how this bacterial pathogen controls these QS systems. Here, we demonstrate a novel two-component system RqpSR (Regulating Quorum sensing and Pathogenicity), which plays an important role in modulating QS and pathogenesis in B...
January 24, 2018: Molecular Microbiology
https://www.readbyqxmd.com/read/29360487/development-of-an-sce-i-mutagenesis-system-for-burkholderia-cepacia-complex-strains
#2
Ashraf Abdu, Gerardo Lara-Juarez, Jonathan J Dennis
The Burkholderia cepacia complex (Bcc) consists of at least 20 phenotypically similar but genotypically distinct Gram-negative bacteria that are ubiquitous in nature, are capable of promoting plant growth and biodegradation of pollutants, but that also are highly antibiotic resistant and produce damaging effects towards plants, fungi, and humans. To study these genetically recalcitrant bacteria in detail, molecular tools are required that work efficiently with the many strains and species of the Bcc. One mutagenesis strategy that has been used effectively to analyze the genes of Burkholderia cenocepacia is based upon the activity of the Sce-I restriction enzyme...
January 19, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29359187/the-small-rna-ncs35-regulates-growth-in-burkholderia-cenocepacia-j2315
#3
Sanne Kiekens, Andrea Sass, Filip Van Nieuwerburgh, Dieter Deforce, Tom Coenye
Burkholderia cenocepacia J2315 is a member of the B. cepacia complex. It has a large genome with three replicons and one plasmid; 7,261 genes code for annotated proteins, while 113 code for functional RNAs. Small regulatory RNAs of B. cenocepacia have not yet been functionally characterized. We investigated a small regulatory RNA, designated ncS35, that was discovered by differential RNA sequencing. Its expression under various conditions was quantified, and a deletion mutant, ΔncS35, was constructed. Compared to planktonic growth in a rich medium, the expression of ncS35 was elevated when B...
January 2018: MSphere
https://www.readbyqxmd.com/read/29322563/microfluidics-based-lc-ms-mrm-approach-for-the-relative-quantification-of-burkholderia-cenocepacia-secreted-virulence-factors
#4
Sofie Depluverez, Simon Daled, Stijn De Waele, Sören Planckaert, Jolien Schoovaerts, Dieter Deforce, Bart Devreese
Burkholderia cenocepacia is an opportunistic pathogen that is commonly isolated from patients with cystic fibrosis (CF). Several virulence factors have been identified, including extracellular enzymes that are secreted by type II and type VI secretion systems. The activity of these secretion systems is modulated by quorum sensing. Apart from the classical acylhomoserine lactone quorum sensing, B. cenocepacia also uses the diffusible signal factor system (DSF) i.e. 2-undecenoic acid derivatives that are recognized by specific receptors resulting in changes in biofilm formation, motility and virulence...
January 11, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29261811/correction-macrophages-but-not-neutrophils-are-critical-for-proliferation-of-burkholderia-cenocepacia-and-ensuing-host-damaging-inflammation
#5
Jennifer Mesureur, Joana R Feliciano, Nelly Wagner, Margarida C Gomes, Lili Zhang, Monica Blanco-Gonzalez, Michiel van der Vaart, David O'Callaghan, Annemarie H Meijer, Annette C Vergunst
[This corrects the article DOI: 10.1371/journal.ppat.1006437.].
December 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29228063/what-matters-in-chronic-burkholderia-cenocepacia-infection-in-cystic-fibrosis-insights-from-comparative-genomics
#6
Jaroslav Nunvar, Vaclav Capek, Karel Fiser, Libor Fila, Pavel Drevinek
Burkholderia cenocepacia causes severe pulmonary infections in cystic fibrosis (CF) patients. Since the bacterium is virtually untreatable by antibiotics, chronic infections persist for years and might develop into fatal septic pneumonia (cepacia syndrome, CS). To devise new strategies to combat chronic B. cenocepacia infections, it is essential to obtain comprehensive knowledge about their pathogenesis. We conducted a comparative genomic analysis of 32 Czech isolates of epidemic clone B. cenocepacia ST32 isolated from various stages of chronic infection in 8 CF patients...
December 11, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29208119/comparative-analysis-of-the-burkholderia-cenocepacia-k56-2-essential-genome-reveals-cell-envelope-functions-that-are-uniquely-required-for-survival-in-species-of-the-genus-burkholderia
#7
April S Gislason, Keith Turner, Mike Domaratzki, Silvia T Cardona
Burkholderia cenocepacia K56-2 belongs to the Burkholderia cepacia complex, a group of Gram-negative opportunistic pathogens that have large and dynamic genomes. In this work, we identified the essential genome of B. cenocepacia K56-2 using high-density transposon mutagenesis and insertion site sequencing (Tn-seq circle). We constructed a library of one million transposon mutants and identified the transposon insertions at an average of one insertion per 27 bp. The probability of gene essentiality was determined by comparing of the insertion density per gene with the variance of neutral datasets generated by Monte Carlo simulations...
November 2017: Microbial Genomics
https://www.readbyqxmd.com/read/29199856/antimicrobial-activity-of-six-essential-oils-against-burkholderia-cepacia-complex-insights-into-mechanism-s-of-action
#8
Elena Perrin, Valentina Maggini, Isabel Maida, Eugenia Gallo, Katia Lombardo, Maria Pia Madarena, Silvia Buroni, Viola Camilla Scoffone, Fabio Firenzuoli, Alessio Mengoni, Renato Fani
AIM: To investigate the activity and mechanisms of action of six essential oils (EOs) against Burkholderia cepacia complex, opportunistic human pathogens highly resistant to antibiotics. MATERIALS & METHODS: Minimal inhibitory concentration of EOs alone, plus antibiotics or efflux pump inhibitors was determined. RESULTS: Origanum vulgare, Thymus vulgaris and Eugenia caryophyllata EOs resulted to be more active than the other EOs. EOs did not enhance antibiotic activity against the model strain B...
December 4, 2017: Future Microbiology
https://www.readbyqxmd.com/read/29164069/iron-acquisition-mechanisms-and-their-role-in-the-virulence-of-burkholderia-species
#9
REVIEW
Aaron T Butt, Mark S Thomas
Burkholderia is a genus within the β-Proteobacteriaceae that contains at least 90 validly named species which can be found in a diverse range of environments. A number of pathogenic species occur within the genus. These include Burkholderia cenocepacia and Burkholderia multivorans, opportunistic pathogens that can infect the lungs of patients with cystic fibrosis, and are members of the Burkholderia cepacia complex (Bcc). Burkholderia pseudomallei is also an opportunistic pathogen, but in contrast to Bcc species it causes the tropical human disease melioidosis, while its close relative Burkholderia mallei is the causative agent of glanders in horses...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29158389/burkholderia-cenocepacia-integrates-cis-2-dodecenoic-acid-and-cyclic-dimeric-guanosine-monophosphate-signals-to-control-virulence
#10
Chunxi Yang, Chaoyu Cui, Qiumian Ye, Jinhong Kan, Shuna Fu, Shihao Song, Yutong Huang, Fei He, Lian-Hui Zhang, Yantao Jia, Yong-Gui Gao, Caroline S Harwood, Yinyue Deng
Quorum sensing (QS) signals are used by bacteria to regulate biological functions in response to cell population densities. Cyclic diguanosine monophosphate (c-di-GMP) regulates cell functions in response to diverse environmental chemical and physical signals that bacteria perceive. In Burkholderia cenocepacia, the QS signal receptor RpfR degrades intracellular c-di-GMP when it senses the QS signal cis-2-dodecenoic acid, also called Burkholderia diffusible signal factor (BDSF), as a proxy for high cell density...
November 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29142288/identification-of-small-rnas-abundant-in-burkholderia-cenocepacia-biofilms-reveal-putative-regulators-with-a-potential-role-in-carbon-and-iron-metabolism
#11
Andrea Sass, Sanne Kiekens, Tom Coenye
Small RNAs play a regulatory role in many central metabolic processes of bacteria, as well as in developmental processes such as biofilm formation. Small RNAs of Burkholderia cenocepacia, an opportunistic pathogenic beta-proteobacterium, are to date not well characterised. To address that, we performed genome-wide transcriptome structure analysis of biofilm grown B. cenocepacia J2315. 41 unannotated short transcripts were identified in intergenic regions of the B. cenocepacia genome. 15 of these short transcripts, highly abundant in biofilms, widely conserved in Burkholderia sp...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29135920/environmental-burkholderia-cenocepacia-strain-enhances-fitness-by-serial-passages-during-long-term-chronic-airways-infection-in-mice
#12
Alessandra Bragonzi, Moira Paroni, Luisa Pirone, Ivan Coladarci, Fiorentina Ascenzioni, Annamaria Bevivino
Burkholderia cenocepacia is an important opportunistic pathogen in cystic fibrosis (CF) patients, and has also been isolated from natural environments. In previous work, we explored the virulence and pathogenic potential of environmental B. cenocepacia strains and demonstrated that they do not differ from clinical strains in some pathogenic traits. Here, we investigated the ability of the environmental B. cenocepacia Mex1 strain, isolated from the maize rhizosphere, to persist and increase its virulence after serial passages in a mouse model of chronic infection...
November 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29130174/identification-of-ahl-and-bdsf-controlled-proteins-in-burkholderia-cenocepacia-by-proteomics
#13
Yilei Liu, Gabriella Pessi, Katharina Riedel, Leo Eberl
We used comparative proteome analysis to determine the target genes of the two quorum sensing (QS) circuits in the opportunistic pathogen Burkholderia cenocepacia: the N-acyl homoserine lactone (AHL)-based CepIR system and the BDSF (B urkholderia diffusible signal factor, cis-2-dodecenoic acid)-based RpfFR system. In this book chapter, we focus on the description of the practical procedure we currently use in the laboratory to perform a sensitive GeLC-MS/MS shotgun proteomics experiment; we also briefly describe the downstream bioinformatic data analysis...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29099689/identification-of-novel-small-rnas-in-burkholderia-cenocepacia-kc-01-expressed-under-iron-limitation-and-oxidative-stress-conditions
#14
Suparna Ghosh, Chetna Dureja, Indu Khatri, Srikrishna Subramanian, Saumya Raychaudhuri, Sagarmoy Ghosh
Small RNA (sRNA)-mediated regulation of gene expression is a major tool to understand bacterial responses to environmental changes. In particular, pathogenic bacteria employ sRNAs to adapt to the host environment and establish infection. Members of the Burkholderia cepacia complex, normally present in soil microbiota, cause nosocomial lung infection especially in hospitalized cystic fibrosis patients. We sequenced the draft genome of Burkholderia cenocepacia KC-01, isolated from the coastal saline soil, and identified several potential sRNAs in silico...
November 3, 2017: Microbiology
https://www.readbyqxmd.com/read/29082233/macrophages-as-drivers-of-an-opportunistic-infection
#15
COMMENT
Annette C Vergunst, Nazareth Lopez Carranza, Lili Zhang, Margarida C Gomes, Yara Tasrini, Annemarie H Meijer, David O'Callaghan
Opportunistic pathogens are a worldwide cause of mortality and morbidity, and infections with intrinsically antibiotic-resistant pathogens have a large clinical, social and economic impact. Bacteria belonging to the Burkholderia cepacia complex (Bcc), ubiquitous in natural and industrial environments, are notorious pathogens for individuals with cystic fibrosis (CF). In addition, Burkholderia cenocepacia is emerging as the culprit of non-CF related, sometimes fatal infections. Knowledge of the underlying infection mechanism of these pathogens is important for efficient treatment, however, to date not much is known about the lifestyle of Bcc bacteria during infection...
September 13, 2017: Microbial Cell
https://www.readbyqxmd.com/read/29055510/prevalence-of-burkholderia-cepacia-complex-species-in-cystic-fibrosis-patients-in-argentina-during-the-period-2011-2015
#16
Lucía Cipolla, Florencia Rocca, Claudia Martinez, Lorena Aguerre, Rubén Barrios, Mónica Prieto
INTRODUCTION: Burkholderia cepacia (B. cepacia) complex is composed of 20 phylogenetically closely related bacterial species. Some species have emerged as opportunistic pathogens in immunocompromised patients and are responsible for nosocomial outbreaks. The B. cepacia complex is a recognized respiratory pathogen in patients with cystic fibrosis. Burkholderia cenocepacia and Burkholderia multivorans (B. multivorans) are the most prevalent species in the world, according to the literature...
October 18, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29032643/effects-of-extended-storage-of-chlorhexidine-gluconate-and-benzalkonium-chloride-solutions-on-the-viability-of-burkholderia-cenocepacia
#17
Youngbeom Ahn, Jeong Myeong Kim, Yong-Jin Lee, John J LiPuma, David Hussong, Bernard S Marasa, Carl E Cerniglia
Chlorhexidine gluconate (CHX) and benzalkonium chloride (BZK) formulations are frequently used as antiseptics in healthcare and consumer products. Burkholderia cepacia complex (BCC) contamination of pharmaceutical products could be due to the use of contaminated water in the manufacturing process, over-diluted antiseptic solutions in the product, and the use of outdated products, which in turn, reduces the antimicrobial activity of CHX and BZK. To establish a "safe use" period following opening containers of CHX and BZK, we measured the antimicrobial effects of CHX (2 ~ 10 µg/ml) and BZK (10 ~ 50 µg/ml) at sub-lethal concentrations on six strains of Burkholderia cenocepacia using chemical and microbiological assays...
October 14, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29022160/a-catalyst-from-burkholderia-cenocepacia-for-efficient-anti-prelog-s-bioreduction-of-3-5-bis-trifluoromethyl-acetophenone
#18
Songzhu Yu, Hongxia Li, Yan Lu, Guojun Zheng
(R)-3, 5-Bis(trifluoromethyl)phenyl ethanol is a key chiral intermediate for the synthesis of aprepitant. Through a genome mining approach, an NADPH-dependent short-chain dehydrogenases derived from Burkholderia cenocepacia (Bc-SDR) was discovered with excellent anti-Prelog's stereoselectivity of reducing 3, 5-bis(trifluoromethyl) acetophenone. The enzyme with 247 amino acids was successfully expressed in Escherichia coli and the molecular weight was about 26 kDa. Optimization of reaction conditions showed that the optimum temperature and pH of the enzyme was 25 °C and pH 7...
October 12, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28982193/cysteamine-mediated-clearance-of-antibiotic-resistant-pathogens-in-human-cystic-fibrosis-macrophages
#19
Chandra L Shrestha, Kaivon D Assani, Hannah Rinehardt, Florentina Albastroiu, Shuzhong Zhang, Richard Shell, Amal O Amer, Larry S Schlesinger, Benjamin T Kopp
Members of the Burkholderia cepacia complex are virulent, multi-drug resistant pathogens that survive and replicate intracellularly in patients with cystic fibrosis (CF). We have discovered that B. cenocepacia cannot be cleared from CF macrophages due to defective autophagy, causing continued systemic inflammation and infection. Defective autophagy in CF is mediated through constitutive reactive oxygen species (ROS) activation of transglutaminase-2 (TG2), which causes the sequestration (accumulation) of essential autophagy initiating proteins...
2017: PloS One
https://www.readbyqxmd.com/read/28965239/burkholderia-cepacia-complex-in-cystic-fibrosis-in-a-brazilian-reference-center
#20
Priscila Dentini, Fernando Augusto Lima Marson, Luciana Cardoso Bonadia, Carmen Sílvia Bertuzzo, Antônio Fernando Ribeiro, Carlos Emílio Levy, José Dirceu Ribeiro
The Burkholderia cepacia complex (BCC) can cause a severe decline in lung function in cystic fibrosis (CF). Our objective was to determine the BCC prevalence and to evaluate its clinical impact on CF. Clinical and laboratory variables were determined for CF patients with BCC (Group-A = 50 patients) and without BCC (Group-B = 134 patients). The microorganisms were identified by biochemical tests, the Vitek2(®)Compact test, recA-PCR and recA-nested-PCR with species-specific primers and DNA sequencing. The patients were evaluated by the Shwachman-Kulczycki score (SKCS), Bhalla score (BS), spirometry and body mass index (BMI)...
December 2017: Medical Microbiology and Immunology
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