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Burkholderia cenocepacia

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https://www.readbyqxmd.com/read/29602945/biosynthesis-of-fragin-is-controlled-by-a-novel-quorum-sensing-signal
#1
Christian Jenul, Simon Sieber, Christophe Daeppen, Anugraha Mathew, Martina Lardi, Gabriella Pessi, Dominic Hoepfner, Markus Neuburger, Anthony Linden, Karl Gademann, Leo Eberl
Members of the diazeniumdiolate class of natural compounds show potential for drug development because of their antifungal, antibacterial, antiviral, and antitumor activities. Yet, their biosynthesis has remained elusive to date. Here, we identify a gene cluster directing the biosynthesis of the diazeniumdiolate compound fragin in Burkholderia cenocepacia H111. We provide evidence that fragin is a metallophore and that metal chelation is the molecular basis of its antifungal activity. A subset of the fragin biosynthetic genes is involved in the synthesis of a previously undescribed cell-to-cell signal molecule, valdiazen...
March 30, 2018: Nature Communications
https://www.readbyqxmd.com/read/29559964/comparative-genomics-of-environmental-and-clinical-burkholderia-cenocepacia-strains-closely-related-to-the-highly-transmissible-epidemic-et12-lineage
#2
Josselin Bodilis, Elodie Denet, Elisabeth Brothier, Arnault Graindorge, Sabine Favre-Bonté, Sylvie Nazaret
The Burkholderia cenocepacia epidemic ET12 lineage belongs to the genomovar IIIA including the reference strain J2315, a highly transmissible epidemic B. cenocepacia lineage. Members of this lineage are able to cause lung infections in immunocompromised and cystic fibrosis patients. In this study, we describe the genome of F01, an environmental B. cenocepacia strain isolated from soil in Burkina Faso that is, to our knowledge, the most closely related strain to this epidemic lineage. A comparative genomic analysis was performed on this new isolate, in association with five clinical and one environmental B...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29559421/in-vitro-antimicrobial-activity-of-ceftolozane-tazobactam-against-p-aeruginosa-and-other-non-fermenting-gram-negative-organisms-in-adults-with-cystic-fibrosis
#3
A Gramegna, B C Millar, F Blasi, J S Elborn, D G Downey, J E Moore
INTRODUCTION: Pulmonary exacerbations in people with Cystic Fibrosis (CF), with chronic Gram-negative pathogens, are associated with reduced survival. These pathogens are usually treated with repeated courses of systemic antibiotics. However there is a linked emergence of multidrug resistant (MDR) pathogens. Ceftolozane/tazobactam is a new cephalosporin/beta-lactamase inhibitor combination that has been demonstrated to have good activity against MDR Pseudomonas aeruginosa. MATERIAL & METHODS: In this study ceftolozane/tazobactam was compared to other commonly used intravenous antibiotics against 193 non-fermenting Gram-negative bacteria isolated from CF sputum specimens, including P...
March 17, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29541628/a-c-di-gmp-modulating-protein-regulates-swimming-motility-of-burkholderia-cenocepacia-in-response-to-arginine-and-glutamate
#4
Brijesh Kumar, John L Sorensen, Silvia T Cardona
Burkholderia cenocepacia is an opportunistic bacterium that can thrive in different environments, including the amino acid-rich mucus of the cystic fibrosis (CF) lung. B. cenocepacia responds to the nutritional conditions that mimic the CF sputum by increasing flagellin expression and swimming motility. Individual amino acids also induce swimming but not flagellin expression. Here, we show that modulation of the second messenger cyclic dimeric guanosine monophosphate (c-di-GMP) levels by the PAS-containing c-di-GMP phosphodiesterase, BCAL1069 (CdpA), regulates the swimming motility of B...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29538493/burkholderia-cepacia-cystic-fibrosis-and-outcomes-following-lung-transplantation-experiences-from-a-single-center-in-brazil
#5
Danila de Souza Carraro, Rafael Medeiros Carraro, Silvia Vidal Campos, Leandro Ryuchi Iuamoto, Karina Andrighetti de Oliveira Braga, Lea Campos de Oliveira, Ester Cerdeira Sabino, Flavia Rossi, Paulo Manuel Pêgo-Fernandes
OBJECTIVES: To evaluate the impact of Burkholderia cepacia complex colonization in cystic fibrosis patients undergoing lung transplantation. METHODS: We prospectively analyzed clinical data and respiratory tract samples (sputum and bronchoalveolar lavage) collected from suppurative lung disease patients between January 2008 and November 2013. We also subtyped different Burkholderia cepacia complex genotypes via DNA sequencing using primers against the recA gene in samples collected between January 2012 and November 2013...
March 12, 2018: Clinics
https://www.readbyqxmd.com/read/29528522/survival-after-lung-transplantation-of-cystic-fibrosis-patients-infected-with-burkholderia-dolosa-genomovar-vi
#6
Ruobing Wang, Sebastian K Welsh, Marie Budev, Hilary Goldberg, Peadar G Noone, David Zaas, Debra Boyer
Cystic fibrosis (CF) with severe lung disease is a well-recognized indication for lung transplantation. Colonization with various organisms in CF patients may impact post-transplant morbidity and mortality. Burkholderia cepacia complex (BCC) is made up of distinct genomovars with significant morbidity and mortality associated with B. cenocepacia (genomovar III) following lung transplant. The outcomes of patients infected with genomovar B. dolosa (genomovar VI) have yet to be described in the literature. We performed a retrospective chart review of all cystic fibrosis patients colonized with B...
March 12, 2018: Clinical Transplantation
https://www.readbyqxmd.com/read/29513134/belfast-agar-a-simple-laboratory-medium-to-separate-pseudomonas-aeruginosa-from-pan-resistant-burkholderia-cenocepacia-isolated-from-the-sputum-of-patients-with-cystic-fibrosis-cf
#7
S Caskey, J E Moore, J McCaughan, J C Rendall
No abstract text is available yet for this article.
March 7, 2018: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/29504898/on-burkholderiales-order-microorganisms-and-cystic-fibrosis-in-russia
#8
Olga L Voronina, Marina S Kunda, Natalia N Ryzhova, Ekaterina I Aksenova, Natalia E Sharapova, Andrey N Semenov, Elena L Amelina, Alexandr G Chuchalin, Alexandr L Gintsburg
BACKGROUND: Microbes infecting cystic fibrosis patients' respiratory tract are important in determining patients' functional status. Representatives of Burkholderiales order are the most dangerous. The goal of our investigation was to reveal the diversity of Burkholderiales, define of their proportion in the microbiome of various parts of respiratory tract and determine the pathogenicity of the main representatives. RESULTS: In more than 500 cystic fibrosis patients, representing all Federal Regions of Russia, 34...
February 9, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29498745/molecular-typing-of-burkholderia-cepacia-complex-isolated-from-patients-attending-an-italian-cystic-fibrosis-centre
#9
Antonio Teri, Samantha Sottotetti, Arianna Biffi, Daniela Girelli, Monica D'Accico, Milena Arghittu, Carla Colombo, Fabiola Corti, Giovanna Pizzamiglio, Lisa Cariani
Bacteria from the Burkholderia cepacia complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). Bcc infection is often extremely difficult to treat due to its intrinsic resistance to multiple antibiotics. In addition, it seems to speed up the decline of lung function and is considered a contraindication for lung transplantation in CF. This study investigates the species of the Bcc strains recovered from chronically infected CF subjects by means of: isolation, identification methods and complete recA nucleotide sequences of 151 samples...
March 2, 2018: New Microbiologica
https://www.readbyqxmd.com/read/29363827/a-novel-two-component-system-modulates-quorum-sensing-and-pathogenicity-in-burkholderia-cenocepacia
#10
Chaoyu Cui, Chunxi Yang, Shihao Song, Shuna Fu, Xiuyun Sun, Liang Yang, Fei He, Lian-Hui Zhang, Yongliang Zhang, Yinyue Deng
Quorum sensing (QS) is widely utilized by bacterial pathogens to regulate biological functions and pathogenicity. Recent evidence has shown that QS is subject to regulatory cascades, especially two-component systems that often respond to environmental stimulation. At least two different types of QS systems regulate pathogenesis in Burkholderia cenocepacia. However, it remains unclear how this bacterial pathogen controls these QS systems. Here, we demonstrate a novel two-component system RqpSR (Regulating Quorum sensing and Pathogenicity), which plays an important role in modulating QS and pathogenesis in B...
January 24, 2018: Molecular Microbiology
https://www.readbyqxmd.com/read/29360487/development-of-an-sce-i-mutagenesis-system-for-burkholderia-cepacia-complex-strains
#11
Ashraf Abdu, Gerardo Lara-Juarez, Jonathan J Dennis
The Burkholderia cepacia complex (Bcc) consists of at least 20 phenotypically similar but genotypically distinct Gram-negative bacteria that are ubiquitous in nature, are capable of promoting plant growth and biodegradation of pollutants, but that also are highly antibiotic resistant and produce damaging effects towards plants, fungi, and humans. To study these genetically recalcitrant bacteria in detail, molecular tools are required that work efficiently with the many strains and species of the Bcc. One mutagenesis strategy that has been used effectively to analyze the genes of Burkholderia cenocepacia is based upon the activity of the Sce-I restriction enzyme...
January 19, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29359187/the-small-rna-ncs35-regulates-growth-in-burkholderia-cenocepacia-j2315
#12
Sanne Kiekens, Andrea Sass, Filip Van Nieuwerburgh, Dieter Deforce, Tom Coenye
Burkholderia cenocepacia J2315 is a member of the B. cepacia complex. It has a large genome with three replicons and one plasmid; 7,261 genes code for annotated proteins, while 113 code for functional RNAs. Small regulatory RNAs of B. cenocepacia have not yet been functionally characterized. We investigated a small regulatory RNA, designated ncS35, that was discovered by differential RNA sequencing. Its expression under various conditions was quantified, and a deletion mutant, ΔncS35, was constructed. Compared to planktonic growth in a rich medium, the expression of ncS35 was elevated when B...
January 2018: MSphere
https://www.readbyqxmd.com/read/29322563/microfluidics-based-lc-ms-mrm-approach-for-the-relative-quantification-of-burkholderia-cenocepacia-secreted-virulence-factors
#13
Sofie Depluverez, Simon Daled, Stijn De Waele, Sören Planckaert, Jolien Schoovaerts, Dieter Deforce, Bart Devreese
Burkholderia cenocepacia is an opportunistic pathogen that is commonly isolated from patients with cystic fibrosis (CF). Several virulence factors have been identified, including extracellular enzymes that are secreted by type II and type VI secretion systems. The activity of these secretion systems is modulated by quorum sensing. Apart from the classical acylhomoserine lactone quorum sensing, B. cenocepacia also uses the diffusible signal factor system (DSF) i.e. 2-undecenoic acid derivatives that are recognized by specific receptors resulting in changes in biofilm formation, motility and virulence...
January 11, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29261811/correction-macrophages-but-not-neutrophils-are-critical-for-proliferation-of-burkholderia-cenocepacia-and-ensuing-host-damaging-inflammation
#14
Jennifer Mesureur, Joana R Feliciano, Nelly Wagner, Margarida C Gomes, Lili Zhang, Monica Blanco-Gonzalez, Michiel van der Vaart, David O'Callaghan, Annemarie H Meijer, Annette C Vergunst
[This corrects the article DOI: 10.1371/journal.ppat.1006437.].
December 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29228063/what-matters-in-chronic-burkholderia-cenocepacia-infection-in-cystic-fibrosis-insights-from-comparative-genomics
#15
Jaroslav Nunvar, Vaclav Capek, Karel Fiser, Libor Fila, Pavel Drevinek
Burkholderia cenocepacia causes severe pulmonary infections in cystic fibrosis (CF) patients. Since the bacterium is virtually untreatable by antibiotics, chronic infections persist for years and might develop into fatal septic pneumonia (cepacia syndrome, CS). To devise new strategies to combat chronic B. cenocepacia infections, it is essential to obtain comprehensive knowledge about their pathogenesis. We conducted a comparative genomic analysis of 32 Czech isolates of epidemic clone B. cenocepacia ST32 isolated from various stages of chronic infection in 8 CF patients...
December 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29208119/comparative-analysis-of-the-burkholderia-cenocepacia-k56-2-essential-genome-reveals-cell-envelope-functions-that-are-uniquely-required-for-survival-in-species-of-the-genus-burkholderia
#16
April S Gislason, Keith Turner, Mike Domaratzki, Silvia T Cardona
Burkholderia cenocepacia K56-2 belongs to the Burkholderia cepacia complex, a group of Gram-negative opportunistic pathogens that have large and dynamic genomes. In this work, we identified the essential genome of B. cenocepacia K56-2 using high-density transposon mutagenesis and insertion site sequencing (Tn-seq circle). We constructed a library of one million transposon mutants and identified the transposon insertions at an average of one insertion per 27 bp. The probability of gene essentiality was determined by comparing of the insertion density per gene with the variance of neutral datasets generated by Monte Carlo simulations...
November 2017: Microbial Genomics
https://www.readbyqxmd.com/read/29199856/antimicrobial-activity-of-six-essential-oils-against-burkholderia-cepacia-complex-insights-into-mechanism-s-of-action
#17
Elena Perrin, Valentina Maggini, Isabel Maida, Eugenia Gallo, Katia Lombardo, Maria Pia Madarena, Silvia Buroni, Viola Camilla Scoffone, Fabio Firenzuoli, Alessio Mengoni, Renato Fani
AIM: To investigate the activity and mechanisms of action of six essential oils (EOs) against Burkholderia cepacia complex, opportunistic human pathogens highly resistant to antibiotics. MATERIALS & METHODS: Minimal inhibitory concentration of EOs alone, plus antibiotics or efflux pump inhibitors was determined. RESULTS: Origanum vulgare, Thymus vulgaris and Eugenia caryophyllata EOs resulted to be more active than the other EOs. EOs did not enhance antibiotic activity against the model strain B...
January 2018: Future Microbiology
https://www.readbyqxmd.com/read/29164069/iron-acquisition-mechanisms-and-their-role-in-the-virulence-of-burkholderia-species
#18
REVIEW
Aaron T Butt, Mark S Thomas
Burkholderia is a genus within the β -Proteobacteriaceae that contains at least 90 validly named species which can be found in a diverse range of environments. A number of pathogenic species occur within the genus. These include Burkholderia cenocepacia and Burkholderia multivorans , opportunistic pathogens that can infect the lungs of patients with cystic fibrosis, and are members of the Burkholderia cepacia complex (Bcc). Burkholderia pseudomallei is also an opportunistic pathogen, but in contrast to Bcc species it causes the tropical human disease melioidosis, while its close relative Burkholderia mallei is the causative agent of glanders in horses...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29158389/-burkholderia-cenocepacia-integrates-cis-2-dodecenoic-acid-and-cyclic-dimeric-guanosine-monophosphate-signals-to-control-virulence
#19
Chunxi Yang, Chaoyu Cui, Qiumian Ye, Jinhong Kan, Shuna Fu, Shihao Song, Yutong Huang, Fei He, Lian-Hui Zhang, Yantao Jia, Yong-Gui Gao, Caroline S Harwood, Yinyue Deng
Quorum sensing (QS) signals are used by bacteria to regulate biological functions in response to cell population densities. Cyclic diguanosine monophosphate (c-di-GMP) regulates cell functions in response to diverse environmental chemical and physical signals that bacteria perceive. In Burkholderia cenocepacia , the QS signal receptor RpfR degrades intracellular c-di-GMP when it senses the QS signal cis -2-dodecenoic acid, also called Burkholderia diffusible signal factor (BDSF), as a proxy for high cell density...
December 5, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29142288/identification-of-small-rnas-abundant-in-burkholderia-cenocepacia-biofilms-reveal-putative-regulators-with-a-potential-role-in-carbon-and-iron-metabolism
#20
Andrea Sass, Sanne Kiekens, Tom Coenye
Small RNAs play a regulatory role in many central metabolic processes of bacteria, as well as in developmental processes such as biofilm formation. Small RNAs of Burkholderia cenocepacia, an opportunistic pathogenic beta-proteobacterium, are to date not well characterised. To address that, we performed genome-wide transcriptome structure analysis of biofilm grown B. cenocepacia J2315. 41 unannotated short transcripts were identified in intergenic regions of the B. cenocepacia genome. 15 of these short transcripts, highly abundant in biofilms, widely conserved in Burkholderia sp...
November 15, 2017: Scientific Reports
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