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Burkholderia cenocepacia

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https://www.readbyqxmd.com/read/28325850/phenotypic-diversity-and-genotypic-flexibility-of-burkholderia-cenocepacia-during-long-term-chronic-infection-of-cystic-fibrosis-lungs
#1
Amy Huei-Yi Lee, Stephane Flibotte, Sunita Sinha, Adrianna Paiero, Rachel L Ehrlich, Sergey Balashov, Garth D Ehrlich, James E A Zlosnik, Joshua Chang Mell, Corey Nislow
Chronic bacterial infections of the lung are the leading cause of morbidity and mortality in cystic fibrosis patients. Tracking bacterial evolution during chronic infections can provide insights into how host selection pressures-including immune responses and therapeutic interventions-shape bacterial genomes. We carried out genomic and phenotypic analyses of 215 serially collected Burkholderia cenocepacia isolates from 16 cystic fibrosis patients, spanning a period of 2-20 yr and a broad range of epidemic lineages...
March 21, 2017: Genome Research
https://www.readbyqxmd.com/read/28296935/peptide-modification-results-in-the-formation-of-a-dimer-with-a-60-fold-enhanced-antimicrobial-activity
#2
Amal Thamri, Myriam Létourneau, Alex Djoboulian, David Chatenet, Eric Déziel, Annie Castonguay, Jonathan Perreault
Cationic antimicrobial peptides (CAMPs) occur naturally in numerous organisms and are considered as a class of antibiotics with promising potential against multi-resistant bacteria. Herein, we report a strategy that can lead to the discovery of novel small CAMPs with greatly enhanced antimicrobial activity and retained antibiofilm potential. We geared our efforts towards i) the N-terminal cysteine functionalization of a previously reported small synthetic cationic peptide (peptide 1037, KRFRIRVRV-NH2), ii) its dimerization through a disulfide bond, and iii) a preliminary antimicrobial activity assessment of the newly prepared dimer against Pseudomonas aeruginosa and Burkholderia cenocepacia, pathogens responsible for the formation of biofilms in lungs of individuals with cystic fibrosis...
2017: PloS One
https://www.readbyqxmd.com/read/28294652/sequential-burkholderia-cenocepacia-isolates-from-siblings-with-cystic-fibrosis-show-increased-lung-cell-attachment
#3
Louise Cullen, Andrew O'Connor, Pavel Drevinek, Kirsten Schaffer, Siobhán McClean
No abstract text is available yet for this article.
March 15, 2017: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/28292982/antibiotic-capture-by-bacterial-lipocalins-uncovers-an-extracellular-mechanism-of-intrinsic-antibiotic-resistance
#4
Omar M El-Halfawy, Javier Klett, Rebecca J Ingram, Slade A Loutet, Michael E P Murphy, Sonsoles Martín-Santamaría, Miguel A Valvano
The potential for microbes to overcome antibiotics of different classes before they reach bacterial cells is largely unexplored. Here we show that a soluble bacterial lipocalin produced by Burkholderia cenocepacia upon exposure to sublethal antibiotic concentrations increases resistance to diverse antibiotics in vitro and in vivo These phenotypes were recapitulated by heterologous expression in B. cenocepacia of lipocalin genes from Pseudomonas aeruginosa, Mycobacterium tuberculosis, and methicillin-resistant Staphylococcus aureus Purified lipocalin bound different classes of bactericidal antibiotics and contributed to bacterial survival in vivo Experimental and X-ray crystal structure-guided computational studies revealed that lipocalins counteract antibiotic action by capturing antibiotics in the extracellular space...
March 14, 2017: MBio
https://www.readbyqxmd.com/read/28258146/a-broad-host-range-tailocin-from-burkholderia-cenocepacia
#5
Guichun W Yao, Iris Duarte, Tram T Le, Lisa Carmody, John J LiPuma, Ry Young, Carlos F Gonzalez
The Burkholderia cepacia complex (Bcc) consists of 20 closely related gram-negative bacterial species that are significant pathogens for persons with cystic fibrosis (CF). Some Bcc strains are highly transmissible and resistant to multiple antibiotics, making infection difficult to treat. A tailocin (phage-tail-like bacteriocin), designated BceTMilo, with broad host range against members of the Bcc was identified in Burkholderia cenocepacia strain BC0425. Sixty-eight percent of Bcc representing 10 species and 90% of non-Bcc Burkholderia strains tested were sensitive to BceTMilo...
March 3, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28248999/evaluation-of-combination-therapy-for-burkholderia-cenocepacia-lung-infection-in-different-in-vitro-and-in-vivo-models
#6
Freija Van den Driessche, Bieke Vanhoutte, Gilles Brackman, Aurélie Crabbé, Petra Rigole, Jurgen Vercruysse, Glenn Verstraete, Davie Cappoen, Chris Vervaet, Paul Cos, Tom Coenye
Burkholderia cenocepacia is an opportunistic pathogen responsible for life-threatening infections in cystic fibrosis patients. B. cenocepacia is extremely resistant towards antibiotics and therapy is complicated by its ability to form biofilms. We investigated the efficacy of an alternative antimicrobial strategy for B. cenocepacia lung infections using in vitro and in vivo models. A screening of the NIH Clinical Collection 1&2 was performed against B. cenocepacia biofilms formed in 96-well microtiter plates in the presence of tobramycin to identify repurposing candidates with potentiator activity...
2017: PloS One
https://www.readbyqxmd.com/read/28178425/synthetic-cystic-fibrosis-sputum-medium-diminishes-i-burkholderia-cenocepacia-i-antifungal-activity-against-i-aspergillus-fumigatus-i-independently-of-phenylacetic-acid-production
#7
Tasia Joy Lightly, Ryan R Phung, John L Sorensen, Silvia T Cardona
Phenylacetic acid (PAA), an intermediate of phenylalanine degradation, is emerging as a signal molecule in microbial interactions with the host. In this work, we explore the presence of phenylalanine and PAA catabolism in three microbial pathogens of the cystic fibrosis (CF) lung microbiome: <i>Pseudomonas aeruginosa</i>, <i>Burkholderia cenocepacia</i> and <i>Aspergillus fumigatus</i>. While <i>in silico</i> analysis of <i>B. cenocepacia</i> J2315 and <i>A...
February 8, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28177793/biocontrol-of-burkholderia-cepacia-complex-bacteria-and-bacterial-phytopathogens-by-bdellovibrio-bacteriovorus
#8
Damian McNeely, Ryan M Chanyi, James S Dooley, John E Moore, Susan F Koval
Bdellovibrio and like organisms are predatory bacteria that have the unusual property of using the cytoplasmic constituents of other Gram-negative bacteria as nutrients. These predators may thus provide an alternative approach to the biocontrol of human and plant pathogens. Predators were isolated on Burkholderia cenocepacia K56-2 and J2315 as prey cells, in enrichment cultures with soil and sewage. Three isolates (DM7C, DM8A, and DM11A) were identified as Bdellovibrio bacteriovorus on the basis of morphology, a periplasmic life cycle, and 16S rRNA gene sequencing...
December 22, 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28177462/mouse-intestinal-niche-cells-express-a-distinct-%C3%AE-1-2-fucosylated-glycan-recognized-by-a-lectin-from-burkholderia-cenocepacia
#9
Daisuke Sugahara, Yuka Kobayashi, Yoshihiro Akimoto, Hayato Kawakami
No abstract text is available yet for this article.
March 15, 2017: Glycobiology
https://www.readbyqxmd.com/read/28093527/human-cystic-fibrosis-macrophages-have-defective-calcium-dependent-protein-kinase-c-activation-of-the-nadph-oxidase-an-effect-augmented-by-burkholderia-cenocepacia
#10
Kaivon Assani, Chandra L Shrestha, Frank Robledo-Avila, Murugesan V Rajaram, Santiago Partida-Sanchez, Larry S Schlesinger, Benjamin T Kopp
Macrophage intracellular pathogen killing is defective in cystic fibrosis (CF), despite abundant production of reactive oxygen species (ROS) in lung tissue. Burkholderia species can cause serious infection in CF and themselves affect key oxidase components in murine non-CF cells. However, it is unknown whether human CF macrophages have an independent defect in the oxidative burst and whether Burkholderia contributes to this defect in terms of assembly of the NADPH oxidase complex and subsequent ROS production...
March 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28060362/cheating-fosters-species-co-existence-in-well-mixed-bacterial-communities
#11
Anne Leinweber, R Fredrik Inglis, Rolf Kümmerli
Explaining the enormous biodiversity observed in bacterial communities is challenging because ecological theory predicts that competition between species occupying the same niche should lead to the exclusion of less competitive community members. Competitive exclusion should be particularly strong when species compete for a single limiting resource or live in unstructured habitats that offer no refuge for weaker competitors. Here, we describe the 'cheating effect', a form of intra-specific competition that can counterbalance between-species competition, thereby fostering biodiversity in unstructured habitats...
January 6, 2017: ISME Journal
https://www.readbyqxmd.com/read/27987042/using-dendritic-cells-to-evaluate-how-burkholderia-cenocepacia-clonal-isolates-from-a-chronically-infected-cystic-fibrosis-patient-subvert-immune-functions
#12
M Guadalupe Cabral, Marília Pereira, Zélia Silva, Inês Iria, Carla Coutinho, Andreia Lopes, Isabel Sá-Correia, Paula A Videira
Infection with Burkholderia cepacia complex (Bcc) bacteria is a threat to cystic fibrosis (CF) patients, commonly leading to a fatal pneumonia, the cepacia syndrome. It causes a massive production of pro-inflammatory cytokines and leucocyte recruitment to airway epithelium without resolving infection and contributing to tissue lesion. To dissect how Bcc bacteria subvert the immune response, we developed a co-culture model with human dendritic cells (DCs) and B. cenocepacia clonal variants isolated from a chronically infected CF patient, who died with cepacia syndrome...
December 16, 2016: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/27979999/mouse-intestinal-niche-cells-express-a-distinct-%C3%AE-1-2-fucosylated-glycan-recognized-by-a-lectin-from-burkholderia-cenocepacia
#13
Daisuke Sugahara, Yuka Kobayashi, Yoshihiro Akimoto, Hayato Kawakami
In this study, we examined the distribution of fucosylated glycans in mouse intestines using a lectin, BC2LCN (N-terminal domain of the lectin BC2L-C from Burkholderia cenocepacia), as a probe. BC2LCN is specific for glycans with a terminal Fucα1,2Galβ1,3-motif and it is a useful marker for discriminating the undifferentiated status of human induced/embryonic stem cells. Apparent BC2LCN reactivity was detected in the secretory granules of goblet cells in the ileum but not those in the colon. We also found distinctive reactivity in the crypt bottom, which is known as the stem cell zone, of the colon and the ileum...
November 22, 2016: Glycobiology
https://www.readbyqxmd.com/read/27898711/biochemical-characterization-of-glutamate-racemase-a-new-candidate-drug-target-against-burkholderia-cenocepacia-infections
#14
Aygun Israyilova, Silvia Buroni, Federico Forneris, Viola Camilla Scoffone, Namiq Q Shixaliyev, Giovanna Riccardi, Laurent Roberto Chiarelli
The greatest obstacle for the treatment of cystic fibrosis patients infected with the Burkholderia species is their intrinsic antibiotic resistance. For this reason, there is a need to develop new effective compounds. Glutamate racemase, an essential enzyme for the biosynthesis of the bacterial cell wall, is an excellent candidate target for the design of new antibacterial drugs. To this aim, we recombinantly produced and characterized glutamate racemase from Burkholderia cenocepacia J2315. From the screening of an in-house library of compounds, two Zn (II) and Mn (III) 1,3,5-triazapentadienate complexes were found to efficiently inhibit the glutamate racemase activity with IC50 values of 35...
2016: PloS One
https://www.readbyqxmd.com/read/27886433/the-burkholderia-cenocepacia-peptidoglycan-associated-lipoprotein-is-involved-in-epithelial-cell-attachment-and-elicitation-of-inflammation
#15
Ruth Dennehy, Maria Romano, Alessia Ruggiero, Yasmine F Mohamed, Simon L Dignam, Cristóbal Mujica Troncoso, Máire Callaghan, Miguel A Valvano, Rita Berisio, Siobhán McClean
The Burkholderia cepacia complex (Bcc) is a group of Gram-negative opportunistic pathogens causing infections in people with cystic fibrosis (CF). Bcc is highly antibiotic resistant, making conventional antibiotic treatment problematic. The identification of novel targets for anti-virulence therapies should improve therapeutic options for infected CF patients. We previously identified that the peptidoglycan-associated lipoprotein (Pal) was immunogenic in Bcc infected CF patients; however, its role in Bcc pathogenesis is unknown...
November 7, 2016: Cellular Microbiology
https://www.readbyqxmd.com/read/27871013/tri-and-tetravalent-mannoclusters-cross-link-and-aggregate-bc2l-a-lectin-from-burkholderia-cenocepacia
#16
Magdolna Csávás, Lenka Malinovská, Florent Perret, Milán Gyurkó, Zita Tünde Illyés, Michaela Wimmerová, Anikó Borbás
The opportunistic Gram-negative bacterium Burkholderia cenocepacia causes lethal infections in cystic fibrosis patients. Multivalent mannoside derivatives were prepared as potential inhibitors of lectin BC2L-A, one of the virulence factors deployed by B. cenocepacia in the infection process. An (α1→2)-thio-linked mannobioside mimic bearing an azide functionalized aglycon was conjugated to different multivalent scaffolds such as propargylated calix[4]arenes, methyl gallate and pentaerythritol by azide-alkyne 1,3-dipolar cycloaddition...
January 2, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/27862204/a-comparison-of-the-immunological-potency-of-burkholderia-lipopolysaccharides-in-endotoxemic-balb-c-mice
#17
Pei-Tan Hsueh, Chiu-Lin Liu, Hsuan-Han Wang, Wei-Fen Ni, Ya-Lei Chen, Jong-Kang Liu
Lipopolysaccharide is one of the virulence factors of the soil-borne pathogens Burkholderia pseudomallei, B. thailandensis, B. cenocepacia and B. multivorans, which cause septic melioidosis (often in B. pseudomallei infections but rarely in B. thailandensis infections) or cepacia syndromes (commonly in B. cenocepacia infections but rarely in B. multivorans infections). The inflammatory responses in Burkholderia LPS-induced endotoxemia were evaluated in this study. Prior to induction, the conserved structures and functions of each purified LPS were determined using electrophoretic phenotypes, the ratios of 3-hydroxytetradecanoic to 3-hydroxyhexadecanoic acid and endotoxin units...
November 2016: Microbiology and Immunology
https://www.readbyqxmd.com/read/27830695/crystal-structure-of-bacterial-haem-importer-complex-in-the-inward-facing-conformation
#18
Youichi Naoe, Nozomi Nakamura, Akihiro Doi, Mia Sawabe, Hiro Nakamura, Yoshitsugu Shiro, Hiroshi Sugimoto
Pathogenic bacteria remove iron from the haem of host tissues and use it as a catalytic center of many enzymes. Haem uptake by pathogenic bacteria is facilitated by the membrane-integrated haem importer, which belongs to the type II ATP-binding cassette (ABC) transporter. Here we present crystal structures of Burkholderia cenocepacia haem importer BhuUV complexed with the periplasmic haem-binding protein BhuT and in the absence of BhuT. The transmembrane helices of these structures show an inward-facing conformation, in which the cytoplasmic gate of the haem translocation pathway is completely open...
November 10, 2016: Nature Communications
https://www.readbyqxmd.com/read/27825973/an-efficient-system-for-the-generation-of-marked-genetic-mutants-in-members-of-the-genus-burkholderia
#19
Sravanthi Shastri, Helena L Spiewak, Aderonke Sofoluwe, Vigdis A Eidsvaag, Atif H Asghar, Tyrone Pereira, Edward H Bull, Aaron T Butt, Mark S Thomas
To elucidate the function of a gene in bacteria it is vital that targeted gene inactivation (allelic replacement) can be achieved. Allelic replacement is often carried out by disruption of the gene of interest by insertion of an antibiotic-resistance marker followed by subsequent transfer of the mutant allele to the genome of the host organism in place of the wild-type gene. However, due to their intrinsic resistance to many antibiotics only selected antibiotic-resistance markers can be used in members of the genus Burkholderia, including the Burkholderia cepacia complex (Bcc)...
January 2017: Plasmid
https://www.readbyqxmd.com/read/27799222/competitive-growth-enhances-conditional-growth-mutant-sensitivity-to-antibiotics-and-exposes-a-two-component-system-as-an-emerging-antibacterial-target-in-burkholderia-cenocepacia
#20
April S Gislason, Matthew Choy, Ruhi A M Bloodworth, Wubin Qu, Maria S Stietz, Xuan Li, Chenggang Zhang, Silvia T Cardona
Chemogenetic approaches to profile an antibiotic mode of action are based on detecting differential sensitivities of engineered bacterial strains in which the antibacterial target (usually encoded by an essential gene) or an associated process is regulated. We previously developed an essential-gene knockdown mutant library in the multidrug-resistant Burkholderia cenocepacia by transposon delivery of a rhamnose-inducible promoter. In this work, we used Illumina sequencing of multiplex-PCR-amplified transposon junctions to track individual mutants during pooled growth in the presence of antibiotics...
January 2017: Antimicrobial Agents and Chemotherapy
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