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Burkholderia cenocepacia

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https://www.readbyqxmd.com/read/27898711/biochemical-characterization-of-glutamate-racemase-a-new-candidate-drug-target-against-burkholderia-cenocepacia-infections
#1
Aygun Israyilova, Silvia Buroni, Federico Forneris, Viola Camilla Scoffone, Namiq Q Shixaliyev, Giovanna Riccardi, Laurent Roberto Chiarelli
The greatest obstacle for the treatment of cystic fibrosis patients infected with the Burkholderia species is their intrinsic antibiotic resistance. For this reason, there is a need to develop new effective compounds. Glutamate racemase, an essential enzyme for the biosynthesis of the bacterial cell wall, is an excellent candidate target for the design of new antibacterial drugs. To this aim, we recombinantly produced and characterized glutamate racemase from Burkholderia cenocepacia J2315. From the screening of an in-house library of compounds, two Zn (II) and Mn (III) 1,3,5-triazapentadienate complexes were found to efficiently inhibit the glutamate racemase activity with IC50 values of 35...
2016: PloS One
https://www.readbyqxmd.com/read/27886433/the-burkholderia-cenocepacia-peptidoglycan-associated-lipoprotein-is-involved-in-epithelial-cell-attachment-and-elicitation-of-inflammation
#2
Ruth Dennehy, Maria Romano, Alessia Ruggiero, Yasmine F Mohamed, Simon L Dignam, Cristóbal Mujica Troncoso, Máire Callaghan, Miguel A Valvano, Rita Berisio, Siobhán McClean
The Burkholderia cepacia complex (Bcc) is a group of Gram-negative opportunistic pathogens causing infections in people with cystic fibrosis (CF). Bcc is highly antibiotic resistant, making conventional antibiotic treatment problematic. The identification of novel targets for anti-virulence therapies should improve therapeutic options for infected CF patients. We previously identified that the peptidoglycan-associated lipoprotein (Pal) was immunogenic in Bcc infected CF patients; however, its role in Bcc pathogenesis is unknown...
November 7, 2016: Cellular Microbiology
https://www.readbyqxmd.com/read/27871013/tri-and-tetravalent-mannoclusters-cross-link-and-aggregate-bc2l-a-lectin-from-burkholderia-cenocepacia
#3
Magdolna Csávás, Lenka Malinovská, Florent Perret, Milán Gyurkó, Zita Tünde Illyés, Michaela Wimmerová, Anikó Borbás
The opportunistic Gram-negative bacterium Burkholderia cenocepacia causes lethal infections in cystic fibrosis patients. Multivalent mannoside derivatives were prepared as potential inhibitors of lectin BC2L-A, one of the virulence factors deployed by B. cenocepacia in the infection process. An (α1→2)-thio-linked mannobioside mimic bearing an azide functionalized aglycon was conjugated to different multivalent scaffolds such as propargylated calix[4]arenes, methyl gallate and pentaerythritol by azide-alkyne 1,3-dipolar cycloaddition...
November 14, 2016: Carbohydrate Research
https://www.readbyqxmd.com/read/27862204/a-comparison-of-the-immunological-potency-of-burkholderia-lipopolysaccharides-in-endotoxemic-balb-c-mice
#4
Pei-Tan Hsueh, Chiu-Lin Liu, Hsuan-Han Wang, Wei-Fen Ni, Ya-Lei Chen, Jong-Kang Liu
Lipopolysaccharide is one of the virulence factors of the soil-borne pathogens Burkholderia pseudomallei, B. thailandensis, B. cenocepacia and B. multivorans, which cause septic melioidosis (often in B. pseudomallei infections but rarely in B. thailandensis infections) or cepacia syndromes (commonly in B. cenocepacia infections but rarely in B. multivorans infections). The inflammatory responses in Burkholderia LPS-induced endotoxemia were evaluated in this study. Prior to induction, the conserved structures and functions of each purified LPS were determined using electrophoretic phenotypes, the ratios of 3-hydroxytetradecanoic to 3-hydroxyhexadecanoic acid and endotoxin units...
November 2016: Microbiology and Immunology
https://www.readbyqxmd.com/read/27830695/crystal-structure-of-bacterial-haem-importer-complex-in-the-inward-facing-conformation
#5
Youichi Naoe, Nozomi Nakamura, Akihiro Doi, Mia Sawabe, Hiro Nakamura, Yoshitsugu Shiro, Hiroshi Sugimoto
Pathogenic bacteria remove iron from the haem of host tissues and use it as a catalytic center of many enzymes. Haem uptake by pathogenic bacteria is facilitated by the membrane-integrated haem importer, which belongs to the type II ATP-binding cassette (ABC) transporter. Here we present crystal structures of Burkholderia cenocepacia haem importer BhuUV complexed with the periplasmic haem-binding protein BhuT and in the absence of BhuT. The transmembrane helices of these structures show an inward-facing conformation, in which the cytoplasmic gate of the haem translocation pathway is completely open...
November 10, 2016: Nature Communications
https://www.readbyqxmd.com/read/27825973/an-efficient-system-for-the-generation-of-marked-genetic-mutants-in-members-of-the-genus-burkholderia
#6
Sravanthi Shastri, Helena L Spiewak, Aderonke Sofoluwe, Vigdis A Eidsvaag, Atif H Asghar, Tyrone Pereira, Edward H Bull, Aaron T Butt, Mark S Thomas
To elucidate the function of a gene in bacteria it is vital that targeted gene inactivation (allelic replacement) can be achieved. Allelic replacement is often carried out by disruption of the gene of interest by insertion of an antibiotic-resistance marker followed by subsequent transfer of the mutant allele to the genome of the host organism in place of the wild-type gene. However, due to their intrinsic resistance to many antibiotics only selected antibiotic-resistance markers can be used in members of the genus Burkholderia, including the Burkholderia cepacia complex (Bcc)...
November 5, 2016: Plasmid
https://www.readbyqxmd.com/read/27799222/competitive-growth-enhances-conditional-growth-mutant-sensitivity-to-antibiotics-and-exposes-a-two-component-system-as-an-emerging-antibacterial-target-in-burkholderia-cenocepacia
#7
April S Gislason, Matthew Choy, Ruhi A M Bloodworth, Wubin Qu, Maria S Stietz, Xuan Li, Chenggang Zhang, Silvia T Cardona
Chemogenetic approaches to profile antibiotic mode of action are based on detecting differential sensitivity of engineered bacterial strains in which the antibacterial target (usually encoded by an essential gene) or an associated process are regulated. We previously developed an essential gene-knockdown mutant library in the multidrug resistant Burkholderia cenocepacia, by transposon-delivery of a rhamnose-inducible promoter. In this work, we used Illumina sequencing of multiplex-PCR amplified transposon junctions to track individual mutants during pooled growth in the presence of antibiotics...
October 31, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27786605/burkholderia-cenocepacia-virulence-microevolution-in-the-cf-lung-variations-on-a-theme
#8
Jonathan J Dennis
No abstract text is available yet for this article.
October 27, 2016: Virulence
https://www.readbyqxmd.com/read/27770178/the-temperate-burkholderia-phage-ap3-of-the-peduovirinae-shows-efficient-antimicrobial-activity-against-b-cenocepacia-of-the-iiia-lineage
#9
Bartosz Roszniowski, Agnieszka Latka, Barbara Maciejewska, Dieter Vandenheuvel, Tomasz Olszak, Yves Briers, Giles S Holt, Miguel A Valvano, Rob Lavigne, Darren L Smith, Zuzanna Drulis-Kawa
Burkholderia phage AP3 (vB_BceM_AP3) is a temperate virus of the Myoviridae and the Peduovirinae subfamily (P2likevirus genus). This phage specifically infects multidrug-resistant clinical Burkholderia cenocepacia lineage IIIA strains commonly isolated from cystic fibrosis patients. AP3 exhibits high pairwise nucleotide identity (61.7 %) to Burkholderia phage KS5, specific to the same B. cenocepacia host, and has 46.7-49.5 % identity to phages infecting other species of Burkholderia. The lysis cassette of these related phages has a similar organization (putative antiholin, putative holin, endolysin, and spanins) and shows 29-98 % homology between specific lysis genes, in contrast to Enterobacteria phage P2, the hallmark phage of this genus...
October 21, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27767197/multilocus-sequence-analysis-reveals-high-genetic-diversity-in-clinical-isolates-of-burkholderia-cepacia-complex-from-india
#10
Vikas Gautam, Prashant P Patil, Sunil Kumar, Samriti Midha, Mandeep Kaur, Satinder Kaur, Meenu Singh, Swapna Mali, Jayanthi Shastri, Anita Arora, Pallab Ray, Prabhu B Patil
Burkholderia cepacia complex (Bcc) is a complex group of bacteria causing opportunistic infections in immunocompromised and cystic fibrosis (CF) patients. Herein, we report multilocus sequence typing and analysis of the 57 clinical isolates of Bcc collected over the period of seven years (2005-2012) from several hospitals across India. A total of 21 sequence types (ST) including two STs from cystic fibrosis patient's isolates and twelve novel STs were identified in the population reflecting the extent of genetic diversity...
October 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27684048/burkholderia-cenocepacia-k56-2-trimeric-autotransporter-adhesin-bcaa-binds-tnfr1-and-contributes-to-induce-airway-inflammation
#11
Dalila Mil-Homens, Sandra N Pinto, Rute G Matos, Cecília Arraiano, Arsenio M Fialho
Chronic lung disease caused by persistent bacterial infections is a major cause of morbidity and mortality in patients with cystic fibrosis (CF). CF pathogens acquire antibiotic resistance, overcame host defenses and impose uncontrolled inflammation that ultimately may cause permanent damage of lungs' airways. Among the multiple CF-associated pathogens, Burkholderia cenocepacia and other B. cepacia complex (Bcc) bacteria have become prominent contributors of disease progression. Here, we demonstrate that BcaA, a trimeric autotransporter adhesin (TAA) from the epidemic strain B...
September 29, 2016: Cellular Microbiology
https://www.readbyqxmd.com/read/27672096/the-fitness-effects-of-spontaneous-mutations-nearly-unseen-by-selection-in-a-bacterium-with-multiple-chromosomes
#12
Marcus M Dillon, Vaughn S Cooper
Mutation accumulation (MA) experiments employ the strategy of minimizing the population size of evolving lineages to greatly reduce effects of selection on newly arising mutations. Thus, most mutations fix within MA lines independently of their fitness effects. This approach, more recently combined with genome sequencing, has detailed the rates, spectra, and biases of different mutational processes. However, a quantitative understanding of the fitness effects of mutations virtually unseen by selection has remained an untapped opportunity...
September 26, 2016: Genetics
https://www.readbyqxmd.com/read/27665523/combination-antimicrobial-susceptibility-testing-of-burkholderia-cepacia-complex-significance-of-species
#13
Felicity K Abbott, Kathleen E N Milne, David A Stead, Ian M Gould
The Burkholderia cepacia complex (Bcc) is notorious for the life-threatening pulmonary infections it causes in patients with cystic fibrosis. The multidrug-resistant nature of Bcc and differing infective Bcc species make the design of appropriate treatment regimens challenging. Previous synergy studies have failed to take account of the species of Bcc isolates. Etest methodology was used to facilitate minimum inhibitory concentration (MIC) and antimicrobial combination testing on 258 isolates of Bcc, identified to species level by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS)...
September 12, 2016: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/27620956/antibiotic-resistance-in-burkholderia-species
#14
Katherine A Rhodes, Herbert P Schweizer
The genus Burkholderia comprises metabolically diverse and adaptable Gram-negative bacteria, which thrive in often adversarial environments. A few members of the genus are prominent opportunistic pathogens. These include Burkholderia mallei and Burkholderia pseudomallei of the B. pseudomallei complex, which cause glanders and melioidosis, respectively. Burkholderia cenocepacia, Burkholderia multivorans, and Burkholderia vietnamiensis belong to the Burkholderia cepacia complex and affect mostly cystic fibrosis patients...
September 2016: Drug Resistance Updates: Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy
https://www.readbyqxmd.com/read/27618991/structural-differences-in-gut-bacteria-communities-in-developmental-stages-of-natural-populations-of-lutzomyia-evansi-from-colombia-s-caribbean-coast
#15
Rafael José Vivero, Natalia Gil Jaramillo, Gloria Cadavid-Restrepo, Sandra I Uribe Soto, Claudia Ximena Moreno Herrera
BACKGROUND: Lutzomyia evansi, a phlebotomine insect endemic to Colombia's Caribbean coast, is considered to be the main vector of visceral and cutaneous leishmaniasis in the region. Although insects of this species can harbor pathogenic and non-pathogenic microorganisms in their intestinal microbiota, there is little information available about the diversity of gut bacteria present in Lutzomyia evansi. In this study, conventional microbiological methods and molecular tools were used to assess the composition of bacterial communities associated with Lutzomyia evansi guts in immature and adult stages of natural populations from the department of Sucre (Caribbean coast of Colombia)...
2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27597847/candidate-essential-genes-in-burkholderia-cenocepacia-j2315-identified-by-genome-wide-tradis
#16
Yee-Chin Wong, Moataz Abd El Ghany, Raeece Naeem, Kok-Wei Lee, Yung-Chie Tan, Arnab Pain, Sheila Nathan
Burkholderia cenocepacia infection often leads to fatal cepacia syndrome in cystic fibrosis patients. However, antibiotic therapy rarely results in complete eradication of the pathogen due to its intrinsic resistance to many clinically available antibiotics. Recent attention has turned to the identification of essential genes as the proteins encoded by these genes may serve as potential targets for development of novel antimicrobials. In this study, we utilized TraDIS (Transposon Directed Insertion-site Sequencing) as a genome-wide screening tool to facilitate the identification of B...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27586172/a-novel-method-for-investigating-burkholderia-cenocepacia-infections-in-patients-with-cystic-fibrosis-and-other-chronic-diseases-of-the-airways
#17
Christiaan D M Wijers, Ryan Vagedes, Christine Weingart
BACKGROUND: Burkholderia cenocepacia is a Gram-negative, opportunistic pathogen that is a cause of morbidity and mortality in patients with cystic fibrosis (CF). Research efforts over the past few decades contributed to our understanding of these infections by identifying virulence factors. However, little is known about how this pathogen adapts to the harsh environment found inside the CF airways, which is characterized by a unique mucus containing high concentrations of inflammatory markers...
2016: BMC Microbiology
https://www.readbyqxmd.com/read/27580679/discovery-of-new-diketopiperazines-inhibiting-burkholderia-cenocepacia-quorum-sensing-in-vitro-and-in-vivo
#18
Viola C Scoffone, Laurent R Chiarelli, Vadim Makarov, Gilles Brackman, Aygun Israyilova, Alberto Azzalin, Federico Forneris, Olga Riabova, Svetlana Savina, Tom Coenye, Giovanna Riccardi, Silvia Buroni
Burkholderia cenocepacia, an opportunistic respiratory pathogen particularly relevant for cystic fibrosis patients, is difficult to eradicate due to its high level of resistance to most clinically relevant antimicrobials. Consequently, the discovery of new antimicrobials as well as molecules capable of inhibiting its virulence is mandatory. In this regard quorum sensing (QS) represents a good target for anti-virulence therapies, as it has been linked to biofilm formation and is important for the production of several virulence factors, including proteases and siderophores...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27541364/elevated-mirc1-mir17-92-cluster-expression-negatively-regulates-autophagy-and-cftr-cystic-fibrosis-transmembrane-conductance-regulator-function-in-cf-macrophages
#19
Mia F Tazi, Duaa A Dakhlallah, Kyle Caution, Madelyn M Gerber, Sheng-Wei Chang, Hany Khalil, Benjamin T Kopp, Amr E Ahmed, Kathrin Krause, Ian Davis, Clay Marsh, Amy E Lovett-Racke, Larry S Schlesinger, Estelle Cormet-Boyaka, Amal O Amer
Cystic fibrosis (CF) is a fatal, genetic disorder that critically affects the lungs and is directly caused by mutations in the CF transmembrane conductance regulator (CFTR) gene, resulting in defective CFTR function. Macroautophagy/autophagy is a highly regulated biological process that provides energy during periods of stress and starvation. Autophagy clears pathogens and dysfunctional protein aggregates within macrophages. However, this process is impaired in CF patients and CF mice, as their macrophages exhibit limited autophagy activity...
November 2016: Autophagy
https://www.readbyqxmd.com/read/27458217/short-palate-lung-and-nasal-epithelial-clone-1-has-antimicrobial-and-antibiofilm-activities-against-the-burkholderia-cepacia-complex
#20
Saira Ahmad, Jean Tyrrell, William G Walton, Ashutosh Tripathy, Matthew R Redinbo, Robert Tarran
The opportunistic bacteria of the Burkholderia cepacia complex (Bcc) are extremely pathogenic to cystic fibrosis (CF) patients, and acquisition of Bcc bacteria is associated with a significant increase in mortality. Treatment of Bcc infections is difficult because the bacteria are multidrug resistant and able to survive in biofilms. Short palate, lung, and nasal epithelial clone 1 (SPLUNC1) is an innate defense protein that is secreted by the upper airways and pharynx. While SPLUNC1 is known to have antimicrobial functions, its effects on Bcc strains are unclear...
October 2016: Antimicrobial Agents and Chemotherapy
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