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Jumei Liu, Ye Zhang, Qianwen Zhao, Bo Situ, Jiamin Zhao, Shihua Luo, Bo Li, Xiaohui Yan, Pankaj Vadgama, Lei Su, Wen Ma, Wen Wang, Lei Zheng
The presence of cancer cells in body fluids confirms the occurrence of metastasis and guides treatment. A simple, fast, and homogeneous fluorescent method was developed to detect cancer cells based on catalytic hairpin assembly (CHA) and bifunctional aptamers. The bifunctional aptamer had a recognition domain for binding to target cancer cells and an initiator domain for triggering the CHA reaction. In the presence of target cells, the bifunctional aptamer was released from the inhibitor and initiated a cascade reaction of assembly and disassembly of the hairpins...
October 31, 2018: Analytica Chimica Acta
Chong Su, Hong Yang, Hui Sun, John Paul Fawcett, Dong Sun, Jingkai Gu
Amphotericin B (AMB) is a polyene macrolide antibiotic used for treating invasive fungal infections. Liposomal AMB (L-AMB) is a lipid dosage form which reduces the side effects and toxicity of the drug. The quantitation of free AMB (F-AMB) and L-AMB in vivo is important to monitor quality control of the liposomal formulation and to ensure its safety during clinical use. In this study, an original strategy was developed to separately determine F-AMB and L-AMB in rat plasma using LC-MS/MS. F-AMB was analyzed after separation by solid phase extraction, total AMB (T-AMB) was determined after protein precipitation and L-AMB was determined by difference...
June 8, 2018: Journal of Pharmaceutical and Biomedical Analysis
Sankeetha Nadarajah
No abstract text is available yet for this article.
June 1, 2018: Bioanalysis
Shudong Zhang, Mingzhi Li, Bin Su, Yuanhua Shao
This review summarizes progress in the fabrication, modification, characterization, and applications of nanopipettes since 2010. A brief history of nanopipettes is introduced, and the details of fabrication, modification, and characterization of nanopipettes are provided. Applications of nanopipettes in chemical analysis are the focus in several cases, including recent progress in imaging; in the study of single molecules, single nanoparticles, and single cells; in fundamental investigations of charge transfer (ion and electron) reactions at liquid/liquid interfaces; and as hyphenated techniques combined with other methods to study the mechanisms of complicated electrochemical reactions and to conduct bioanalysis...
June 12, 2018: Annual Review of Analytical Chemistry
Yifan Shi, Matthew M Rankin, Lisa D Norquay, Naidong Weng, Shefali Patel
Sulprostone is a potent prostaglandin E2 (PGE2 ) analogue and one of the first identified selective G-protein-coupled receptor 3 (EP3 ) agonists. It has been investigated as a potential antiulcer agent and frequently used in the research of EP3 antagonist. To assist pharmacokinetic and pharmacodynamic studies, a rapid and sensitive LC-MS/MS method was developed and qualified for the quantitation of sulprostone in monkey plasma. Using electrospray ionization mass spectrometry, an ammonium adduct in positive mode was chosen for analysis which had seven times of the sensitivity of the depronated ion in negative mode...
May 25, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Hanna V Bandarenka, Kseniya V Girel, Sergey A Zavatski, Andrei Panarin, Sergei N Terekhov
The present work gives an overview of the developments in surface-enhanced Raman scattering (SERS) with metal-coated porous silicon used as an active substrate. We focused this review on the research referenced to SERS-active materials based on porous silicon, beginning from the patent application in 2002 and enclosing the studies of this year. Porous silicon and metal deposition technologies are discussed. Since the earliest studies, a number of fundamentally different plasmonic nanostructures including metallic dendrites, quasi-ordered arrays of metallic nanoparticles (NPs), and metallic nanovoids have been grown on porous silicon, defined by the morphology of this host material...
May 21, 2018: Materials
Mengjie Li, Chuan Xiong, Ying-Ning Zheng, Wen-Bin Liang, Ruo Yuan, Yaqin Chai
Usually, photoelectrochemical (PEC) assays were devoted to the direct modification of photoactive materials on sensing interface, thereby producing high initial signal and unneglected background noise, which could further result in low sensitivity and restricted detection limit during the detection of targets. In this work, a PEC biosensor with near-zero background noise was established for ultrasensitive microRNA-141 (miRNA-141) detection based on DNA tetrahedron (TET) as nanocarrier for efficient immobilization of CdTe quantum dots (QDs)-Methylene Blue (MB) (TET-QDs-MB complex) as signal probe...
June 8, 2018: Analytical Chemistry
Ying Zhuo, Hai-Jun Wang, Yan-Mei Lei, Pu Zhang, Jia-Li Liu, Ya-Qin Chai, Ruo Yuan
Electrochemiluminescence (ECL) has attracted much attention in various fields of analysis owing to low background signals, high sensitivity, and excellent controllability. In recent years, to further boost the performance of biosensors, diverse output signal modes have been developed, which exhibited respective advantages. In this review, we summarize the latest sensing applications of ECL bioanalysis by generalizing different output signal modes and give future perspectives for new developments in ECL analytical technology...
June 7, 2018: Analyst
Erhu Xiong, Xiaoxia Yan, Xiaohua Zhang, Yanmei Li, Ruiying Yang, Leixia Meng, Jinhua Chen
The sensitive and specific determination of nucleic acids is very important in clinical diagnosis and biological studies. In this work, an ultrasensitive photoelectrochemical (PEC) biosensor has been developed for DNA detection based on a "signal-on" sensing strategy and a three-stage cascade signal amplification method (catalytic hairpin assembly (CHA), hybridization chain reaction (HCR) and alkaline phosphatase (ALP)-triggered in situ generation of ascorbic acid (AA)). Here, CHA hairpin 1 (CHA-HP1) is opened by the target DNA (T-DNA) owing to the hybridization between T-DNA and CHA-HP1, and then the opened CHA-HP1 hybridizes with CHA hairpin 2 (CHA-HP2) to displace the T-DNA, generating a CHA-HP1/CHA-HP2 complex...
June 4, 2018: Analyst
Huiyun Yang, Haijun Wang, Chengyi Xiong, Yaqin Chai, Ruo Yuan
In this work, poly[9,9-dioctylfluorenyl-2,7-diyl] (PFO) dots is discovered to display an appealing dual enhancement effect for the electrochemiluminescence (ECL) system of N-(aminobutyl)-N-(ethylisoluminol)/hydrogen peroxide (ABEI/H2 O2 ), which not only enhances the ECL intensity of ABEI but also catalyzes decomposition of H2 O2 to further amplify the ECL signal of ABEI. Owing to the electronegative property of PFO dots, electropositive ABEI-PEI as ECL reagent could be adsorbed on their surface and thus form a novel luminescence emitter (ABEI-PEI-PFO dots) with high ECL efficiency based on electrostatic attraction...
May 22, 2018: Biosensors & Bioelectronics
Shenglong Liao, Yi Tao, Wenbin Du, Yapei Wang
Generation of uniform droplets has been extensively investigated owing to its profound potentials both in scientific research and engineering applications. Although various methods have been put forward to expand this area, new innovations are still needed to improve the technical convenience and save instrumental cost. In this feature article, we highlight an Interfacial Emulsification technique that we developed in the past several years. This technique serves as a platform for preparing uniform droplets that are formed on the air-liquid interface of the continuous phase based on interfacial shearing...
May 24, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Yinfang Wang, Haifeng Sha, Hong Ke, Nengqin Jia
An ultrasensitive electrochemiluminescence (ECL) immunosensor was initially developed for quantitative detection of carbohydrate antigen 15-3 (CA15-3) using platinum nickel nanocubes-L-cysteine-luminol nanocomposite (PtNi NCs-L-Cys-luminol) as signal probe. Herein, the PtNi NCs-L-Cys-luminol nanocomposite was modified on the glassy carbon electrode (GCE) surface via the film-forming properties of chitosan. Then, the CA15-3 antibody was attached to the modified electrode surface by amidating reaction to construct the ECL immunosensor...
August 15, 2018: Talanta
Hongmei Yang, Yan Zhang, Lina Zhang, Kang Cui, Shenguang Ge, Jiadong Huang, Jinghua Yu
Highly conductive, robust, and multifunctional integrated paper-supported electrodes are requisite to fulfill the promise of paper-based analytical application. Herein, an all-in-one Au electrode comprising of detection zone, waterproof electronic bridge, and signal output contactor was engineered via combining the double-sided growth method with the secondary wax-printing. Benefiting from the strongly omnidirectional conductivity and desirably mechanical robustness of the as-prepared electrode, a stackable lab-on-paper cyto-device integrated with high-efficiency photoelectrochemical strategy was developed for the MCF-7 cells assay...
May 21, 2018: Analytical Chemistry
J J M Rood, P J A Dormans, M J van Haren, J H M Schellens, J H Beijnen, R W Sparidans
Ibrutinib is a targeted covalent inhibitor frequently used for the treatment of various lymphomas. In addition to oxidative metabolism, it is metabolized through glutathione coupling. The quantitative insight into this kind of metabolism is scarce, and tools for quantitation are lacking. The non-oxidative metabolism could prove a more prominent role when oxidative metabolism is impaired. Also, in-vitro studies could over-estimate the effect of CYP450-inhibition. To gain quantitative insight into this relatively unknown biotransformation pathway of the drug we have developed a validated simple, fast and sensitive bio-analytical assay for ibrutinib, dihydrodiol-ibrutinib, and the glutathione, cysteinylglycine and cysteine conjugates of ibrutinib in human plasma...
May 11, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Bangrong Lu, Qinghua He, Yonghong He, Xuejing Chen, Guangxia Feng, Siyu Liu, Yanhong Ji
To achieve the dual-channel (analog and digital) encoding, microbeads assembled with quantum dots (QDs) and element coding nanoparticles (ECNPs) have been prepared. Dual-spectra, including fluorescence generated from quantum dots (QDs) and laser induced breakdown spectrum obtained from the plasma of ECNPs, including AgO, MgO and ZnO nanoparticles, has been adopted to provide more encoding amounts and more accurate dual recognition for encoded microbeads in multiplexed utilization. The experimental results demonstrate that the single microbead can be decoded in two optical channels...
September 18, 2018: Analytica Chimica Acta
Jie Pang, Yun Zhao, Hai-Ling Liu, Kang Wang
This paper reported a new method to observe the catalytic progress of the natural horseradish peroxidase (HRP) in-situ on single gold nanoparticles (GNPs) by the combination of dark field imaging and plasmonic resonance scattering spectra. The produced single HRP-GNP exhibited localized catalytic property toward H2 O2 -Diaminobenzidine (DAB), which could be used to detect the concentration of H2 O2 in micro/nanospace. The linear range for H2 O2 sensing was from 0.01 μM to 5 μM with a detection limit of 10 nM...
August 1, 2018: Talanta
Long Yuan, Shenita Basdeo, Qin C Ji
BMS-986104 is a S1P1 R modulator drug candidate under development and has been evaluated in Phase I clinical trials. BMS-986104 functions as a prodrug and undergoes enzymatic transformations in vivo to form the pharmacologically active phosphate drug, BMS-986104-P. Here, we report approaches to overcome the stability, solubility and extraction challenges in developing a sensitive, accurate and rugged LC-MS/MS method for the simultaneous quantification of the phosphate drug and its prodrug in blood lysate. An effective stabilization strategy using a cocktail of phosphatase and kinase inhibitors was developed to ensure the stability of both analytes during sample collection, storage, and processing...
May 5, 2018: Journal of Pharmaceutical and Biomedical Analysis
Katharina Brassat, Saminathan Ramakrishnan, Julius Bürger, Marcel Hanke, Mahnaz Doostdar, Jörg K N Lindner, Guido Grundmeier, Adrian Keller
DNA origami nanostructures are versatile substrates for the controlled arrangement of molecular capture sites with nanometer precision and thus have many promising applications in single-molecule bioanalysis. Here, we investigate the adsorption of DNA origami nanostructures in nanohole arrays which represent an important class of biosensors and may benefit from the incorporation of DNA origami-based molecular probes. Nanoholes with well-defined diameter that enable the adsorption of single DNA origami triangles are fabricated in Au films on Si wafers by nanosphere lithography...
May 13, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Xiaoyan Li, Gang Peng, Feng Cui, Qianying Qiu, Xiaojun Chen, He Huang
An ultrasensitive electrochemical genosensor has been fabricated for the double determination of two different specific sequences deduced from the maternally expressed gene3 (MEG3) lncRNA (long noncoding RNA), which was demonstrated by coupling RNase A-aided target recycling with DNA supersandwich-induced signal enhancement, based on a composite interface of graphene-like tungsten disulfide/dendritic gold nanostructures (WS2 /DGN). Firstly, duple target sequences of T1 and T2 were captured by the primer probes of P1 /P2 functionalized Fe3 O4 @C magnetic nanoparticles, via the DNA/RNA hybridization between T1 /T2 and P1 /P2 ...
May 2, 2018: Biosensors & Bioelectronics
Hamza Kandoussi, Jianing Zeng, Kumar Shah, Patricia Paterson, Rasa Santockyte, Pathanjali Kadiyala, Hong Shen, Petia Shipkova, Robert Langish, Richard Burrrell, John Easter, Thomas Mariannino, Punit Marathe, Yurong Lai, Yan Zhang, Renuka Pillutla
AIM: Coproporphyrins (CP-I and CP-III) have been identified as possible biomarkers to predict human hepatic organic anion-transporting polypeptides-mediated-drug-interactions for a new drug entering clinical development. RESULTS: The method is applicable to quantify plasma CP-I and CP-III within 0.078-15.0 nM. The results identify and address a number of challenges encountered with porphyrin assays such as photodegradation and interferences. To overcome interferences from ubiquitous porphyrins, a surrogate matrix was used to prepare calibration standards...
May 11, 2018: Bioanalysis
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