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Wen-Jing Bao, Jian Li, Jin Li, Qian-Wen Zhang, Yang Liu, Cai-Feng Shi, Xing-Hua Xia
A versatile and sensitive platform for label-free bioanalysis has been proposed based on attenuated total reflection-surface enhanced infrared absorption spectroscopy (ATR-SEIRAS) using Au/ZnSe as the enhancement substrate that allows wide spectral range down to 700 cm-1. Au nanoparticles are stably deposited on the surface of ZnSe prism due to the formation of Au-Se bond via electroless deposition, and the enhancement factor of the resultant Au/ZnSe substrate is about two times larger than that of the commonly used Au/Si substrate...
February 19, 2018: Analytical Chemistry
Maria Myzithras, Tammy Bigwarfe, Erica Waltz, Hua Li, Jennifer Ahlberg, Irina Rybina, Sarah Low, Cynthia Hess Kenny, John Miglietta, Rachel Kroe-Barrett
AIM: The fully automated microfluidics-based Gyrolab is a popular instrument for the bioanalysis of protein therapeutics; requiring minimal sample and reagent volumes. Gyros offers affinity software for determining binding affinity in solution using a high-throughput method and miniaturized reactions. RESULTS: Using this affinity software, multiple CTGF-targeting reagents were characterized on the Gyrolab after <100% target coverage was seen in a cynomolgus pharmacokinetic/PD study dosed with anti-CTGF antibodies...
February 16, 2018: Bioanalysis
Yarmarly C Guerra Valero, Steven C Wallis, Jeffrey Lipman, Christophe Stove, Jason A Roberts, Suzanne L Parker
Conventional sampling techniques for clinical pharmacokinetic studies often require the removal of large blood volumes from patients. This can result in a physiological or emotional burden, particularly for neonates or pediatric patients. Antibiotic pharmacokinetic studies are typically performed on healthy adults or general ward patients. These may not account for alterations to a patient's pathophysiology and can lead to suboptimal treatment. Microsampling offers an important opportunity for clinical pharmacokinetic studies in vulnerable patient populations, where smaller sample volumes can be collected...
February 16, 2018: Bioanalysis
Jun Qu
Jun Qu speaks to Sankeetha Nadarajah, Editor of Bioanalysis: Jun Qu is the group leader of the proteomics and pharmaceutical analysis lab of SUNY-Buffalo (NY, USA) and a Professor in the Department of Pharmaceutical Sciences. His research is focused on the study of Clinical and Pharmaceutical Proteomics and Pharmaceutical Analysis using LC-MS-based strategies. His research programs include high-resolution and large-scale expression profiling of pathological proteomes, for the discovery of novel disease/therapeutics biomarkers using gel-free proteomic methods; sensitive identification, localization and quantification of post-translational modifications in tissue proteomes such as these in myocardium, using novel anti-PTM affinity capture and alternating Collision-induced dissociation/Electron transfer dissociation to obtain abundant PTM information; targeted investigation of marker proteins that are of high interests for clinical and pharmaceutical study, using highly sensitive nano-LC/SRM-based methods; and highly sensitive and accurate investigation of the PK of biotherapeutics using LC-MS...
February 16, 2018: Bioanalysis
Fengzhou Xu, Lan Luo, Hui Shi, Xiaoxiao He, Yanli Lei, Jinlu Tang, Dinggeng He, Zhenzhen Qiao, Kemin Wang
Poly(thymine)-hosted copper nanoparticles (poly T-CuNPs) have emerged as a promising label-free fluorophore for bioanalysis, but its application in RNA-related studies is still rarely explored. Herein, by utilizing duplex-specific nuclease (DSN) as a convertor to integrate target recycling mechanism into terminal deoxynucleotidyl transferase (TdT)-mediated superlong poly T-CuNPs platform, a specific and sensitive method for microRNA detection has been developed. In this strategy, a 3'-phosphorylated DNA probe can hybridize with target RNA and then be cut by DSN to produce 3'-hydroxylated fragments, which can be further tailed by TdT with superlong poly T for fluorescent CuNPs synthesis...
June 20, 2018: Analytica Chimica Acta
Kevin D Clark, María J Trujillo-Rodríguez, Jared L Anderson
Ionic liquids are a class of solvents and materials that hold great promise in bioanalytical chemistry. Task-specific ionic liquids have recently been designed for the selective extraction, separation, and detection of proteins, peptides, nucleic acids, and other physiologically relevant analytes from complex biological samples. To facilitate rapid bioanalysis, ionic liquids have been integrated in miniaturized and automated procedures. Bioanalytical separations have also benefited from the modification of nonspecific magnetic materials with ionic liquids or the implementation of ionic liquids with inherent magnetic properties...
February 12, 2018: Analytical and Bioanalytical Chemistry
Ying Zhou, Yanqing Yu, Yaqin Chai, Ruo Yuan
Herein, a novel electrochemiluminescence resonance energy transfer (ERET) strategy between in situ electrogenerated silver nanoclusters (AgNCs) as the donor and Ru(bpy)2(5-NH2-1,10-phen)Cl2 (Ru(II)) as the acceptor was reported, which was applied for the construction of a ultrasensitive immunosensor for the determination of apolipoprotein-A1 (Apo-A1). Notably, the Ag NCs were in suit electro-reducted on glassy carbon electrode (GCE) based on a simple, fast and controllable electrochemical technique, which acted as not only biocompatible ECL emitters, but also protein immobilization platform via Ag-N or Ag-S bond...
May 1, 2018: Talanta
Jianyuan Dai, Zhijuan Duan, Mengzhu Cao, Mengru Hao, Hongfei He, Dan Xiao
A rapid signal amplified DNA detection method based on self-replicating catalyzed hairpin assembly (SRCHA) has been proposed. In this SRCHA system, two split target DNA sequences were respectively integrated into hairpin auxiliary probes H1 and H2. H2 was used as fluorescent probe which containing a fluorescent nucleotide base analog pyrrolo-deoxycytidine (P-dC) at the end of the stem. Target DNA can be circularly used in this SRCHA system to form the helix DNA H1-H2 complex, the structure change of H2 will move P-dC from hairpin stem to flexible ssDNA sticky end, leading to fluorescence increase due to the less stacking interaction...
May 1, 2018: Talanta
Günter Gauglitz
No abstract text is available yet for this article.
February 8, 2018: Analytical and Bioanalytical Chemistry
Yinzhu Wang, Si-Yuan Ji, Heng-Yu Xu, Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen
A selective excitation of [Ir(df-ppy)2(pic)] and [Ru(bpy)3]2+ through tuning electrode potential is reported in this work. Bidirectional color change from blue-green to red could be observed along with increase and decrease of the potential, which was ascribed to the dual-potential excitation property of [Ir(df-ppy)2(pic)]. Similar to three-electrode system, selective excitation of ECL could be achieved at the anode of bipolar electrode (BPE). Both increase and decrease of the faradic reactions at the cathode of the BPE could induce ECL reporting color at the other pole switched from blue-green to red...
February 8, 2018: Analytical Chemistry
Hyungki Kim, Tiffany Jeen, Michael V Tran, W Russ Algar
Colloidal semiconductor quantum dot (QD) nanocrystals have ideal fluorescence properties for bioanalysis and bioimaging, but these materials must be functionalized with an inorganic shell, organic ligand or polymer coating, and conjugated with biomolecules to be useful in such applications. Several different analytical techniques are used to characterize QDs and their multiple layers of functionalization. Here, we revisit poly(acrylamide) gel electrophoresis (PAGE), which has been scarcely used for the characterization of QDs and their bioconjugates in deference to the routine use of agarose gel electrophoresis...
February 1, 2018: Analyst
Lea Wagmann, Hans H Maurer
Bioanalysis of new psychoactive substances (NPS) is very challenging due to the growing number of compounds with new chemical structures found on the drugs of abuse market. Screening, identification, and quantification in biosamples are needed in clinical and forensic toxicology settings, and these procedures are more challenging than the analysis of seized drug material because of extremely low concentrations encountered in biofluids but also due to diverse metabolic alterations of the parent compounds. This article focuses on bioanalytical single- and multi-analyte procedures applicable to a broad variety of NPS in various biomatrices, such as blood, urine, oral fluid, or hair...
January 28, 2018: Handbook of Experimental Pharmacology
Li-Ping Mei, Xin-Yuan Jiang, Xiao-Dong Yu, Wei-Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen
Herein we report the strategy of liposome-mediated Cu2+-induced exciton trapping upon CdS quantum dots (QDs) for amplified photoelectrochemical (PEC) bioanalysis application. Specifically, the Cu nanoclusters (NCs)-encapsulated liposomes were firstly fabricated and then processed with antibodies bound to their external surfaces. After the sandwich immunocomplexing, the confined liposomal labels were subjected to sequential lysis treatments for the release of Cu NCs and numerous Cu2+ ions, which were then directed to interact with the CdS QDs electrode...
January 23, 2018: Analytical Chemistry
Tianming Ren, Runzhi Li, Xiangjun Meng, J Paul Fawcett, Dong Sun, Jingkai Gu
Eptifibatide is a therapeutic cyclic peptide with poor collision-induced dissociation (CID) efficiency for multiple reaction monitoring (MRM), which limits the development of a traditional liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioassay with MRM. In this study, a method combining differential mobility spectrometry (DMS) with liquid chromatography-multiple ion monitoring (LC-DMS-MIM) was developed for the quantitation of eptifibatide in rat plasma. After solid phase extraction (SPE) of 100 μL plasma on an Oasis® HLB cartridge, the analyte and I...
January 11, 2018: Journal of Pharmaceutical and Biomedical Analysis
Yang Hu, Wenwen Zhu, Yeli Guan, Sanlan Wu, Xiaoyin Zhang, Gao Li, Luqin Si, Jiangeng Huang
A rapid, robust and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for bioanalysis of TJ0711, a novel vasodilatory β-blocker in dog plasma. This assay is able to chromatographically separate TJ0711 from its isobaric metabolite as well as glucuronide conjugates. Chromatographic separation was achieved on a Welch Ultimate-XB C18 column (2.1 × 100 mm, 3 μm). The analyte and internal standard (IS, propranolol) were extracted from plasma by liquid-liquid extraction using ethyl acetate...
January 22, 2018: Biomedical Chromatography: BMC
Bang Lin Li, Li Yu Peng, Hao Lin Zou, Ling Jie Li, Hong Qun Luo, Nian Bing Li
Liquid-phase exfoliation routes unavoidably generate 2D nanostructures with inhomogeneous morphologies. Herein, thickness-dependent sorting of exfoliated nanostructures is achieved via a treatment of differential-zone centrifugation in the surfactant aqueous phase. With this approach, homogeneous MoS2 nanosheets are obtained, and due to the intrinsic semiconducting characteristics, those 2D nanosheets are endowed with desired optical properties, rivaling classic gold nanoparticles in sensing applications. Furthermore, MoS2 nanosheets with high uniformity and chemical inertness are coupled with proteins, exhibiting high performance in stability and anti-interferences for bioanalysis...
January 22, 2018: Small
Armelle Hebert, Cedric Feliers, Caroline Lecarpentier, Peta A Neale, Rita Schlichting, Sylvie Thibert, Beate I Escher
Drinking water can contain low levels of micropollutants, as well as disinfection by-products (DBPs) that form from the reaction of disinfectants with organic and inorganic matter in water. Due to the complex mixture of trace chemicals in drinking water, targeted chemical analysis alone is not sufficient for monitoring. The current study aimed to apply in vitro bioassays indicative of adaptive stress responses to monitor the toxicological profiles and the formation of DBPs in three drinking water distribution systems in France...
January 3, 2018: Water Research
Mingsha Zhao, Shanshan Zhang, Zhiqiang Chen, Changzhi Zhao, Li Wang, Shufeng Liu
Herein, an allosteric kissing complex-based electrochemical biosensor was ingeniously proposed for the simple, sensitive, regenerative and versatile detection of proteins. Two hairpins (Hp1 and Hp2) were designed and the Hp1 was immobilized on the electrode surface, which could form a kissing complex with Hp2 through the apical loop-loop or kissing interaction of the RNA-RNA base sequences. The Hp2 possesses the appended single-stranded tails on each end, which hybridize with the recognition element-conjugated DNA strands to construct a protein responsive switch of Hp2 scaffold...
January 9, 2018: Biosensors & Bioelectronics
Lucyna Konieczna, Anna Roszkowska, Teresa Stachowicz-Stencel, Anna Synakiewicz, Tomasz Bączek
This paper details the quantitative analysis of neurotransmitters, including dopamine (DA), norepinephrine (NE), epinephrine (E), and serotonin (5-HT), along with their respective precursors and metabolites in children with solid tumors: Wilms' tumor (WT) and neuroblastoma (NB). A panel of neurotransmitters was determined with the use of dispersive liquid-liquid microextraction (DLLME) technique combined with liquid-chromatography mass spectrometry (LC-MS/MS) in plasma samples obtained from a group of pediatric subjects with solid tumors and a control group of healthy children...
December 28, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Dan Li, Gang Zhao, Weipeng Ai, Gao Li, Luqin Si, Jiangeng Huang, Yunzhou Chen, Sanlan Wu
In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously determine the anticancer drugs etoposide and paclitaxel in mouse plasma and tissues including liver, kidney, lung, heart, spleen and brain. The analytes were extracted from the matrices of interest by liquid-liquid extraction using DCM: MTBE (1:1, v/v). Chromatographic separation was achieved on an Ultimate XB-C18 column (100 mm × 2.1 mm, 3 μm) at 40 °C and the total run time was 4 min under a gradient elution...
January 19, 2018: Biomedical Chromatography: BMC
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