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https://www.readbyqxmd.com/read/28445850/simultaneous-quantification-of-busulfan-clofarabine-and-f-ara-a-using-isotope-labelled-standards-and-standard-addition-in-plasma-by-lc-ms-ms-for-exposure-monitoring-in-hematopoietic-cell-transplantation-conditioning
#1
Arjen M Punt, Jurgen B Langenhorst, Annelies C Egas, Jaap Jan Boelens, Charlotte van Kesteren, Erik M van Maarseveen
In allogeneic hematopoietic cell transplantation (HCT) it has been shown that over- or underexposure to conditioning agents have an impact on patient outcomes. Conditioning regimens combining busulfan (Bu) and fludarabine (Flu) with or without clofarabine (Clo) are gaining interest worldwide in HCT. To evaluate and possibly adjust full conditioning exposure a simultaneous analysis of Bu, F-ARA-A (active metabolite of Flu) and Clo in one analytical run would be of great interest. However, this is a chromatographical challenge due to the large structural differences of Bu compared to F-ARA-A and Clo...
April 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28432695/recent-strategies-towards-microfluidic-based-surface-enhanced-raman-spectroscopy
#2
REVIEW
Anna Tycova, Jan Prikryl, Frantisek Foret
Surface enhanced Raman spectroscopy (SERS) is an extremely powerful analytical tool, which not only yields information about the molecular structure of the analyte in the form of characteristic vibrational spectrum but also gives sensitivities approaching those in fluorescence spectroscopy. The SERS measurement on the microfluidic platform provides possibility to manufacture the device with design perfectly fulfilling the needs of the application with minimal sample consumption. This review aims at describing basic strategies for SERS measurement in microfluidic devices published in the last decade and covers current trends in microfluidics with SERS detection in the field of bioanalysis and approaches towards on-line coupling of liquid-based separation techniques with SERS detection...
April 22, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28426136/differentiating-signals-to-make-biological-sense-a-guide-through-databases-for-ms-based-non-targeted-metabolomics
#3
REVIEW
Alberto Gil de la Fuente, Emily Grace Armitage, Abraham Otero, Coral Barbas, Joanna Godzien
Metabolite identification is one of the most challenging steps in metabolomics studies and reflects one of the greatest bottlenecks in the entire workflow. The success of this step determines the success of the entire research, therefore the quality at which annotations are given requires special attention. A variety of tools and resources are available to aid metabolite identification or annotation, offering different and often complementary functionalities. In preparation for this article, almost 50 databases were reviewed, from which 17 were selected for discussion, chosen for their on-line ESI-MS functionality...
April 20, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28406432/a-new-strategy-for-silver-deposition-on-au-nanoparticles-with-the-use-of-peroxidase-mimicking-dnazyme-monitored-via-a-localized-surface-plasmon-resonance-technique
#4
Joanna Kosman, Jacqueline Jatschka, Andrea Csaki, Wolfgang Fritzsche, Bernard Juskowiak, Ondrej Stranik
Peroxidase-mimicking DNAzyme was applied as a catalyst of silver deposition on gold nanoparticles. This DNAzyme is formed when hemin binds to the G-quadruplex-forming DNA sequence. Such a system is able to catalyze a redox reaction with a one- or two-electron transfer. The process of silver deposition was monitored via a localized surface plasmon resonance technique (LSPR), which allows one to record scattering spectrum of a single nanoparticle. Our study showed that DNAzyme is able to catalyze silver deposition...
April 13, 2017: Sensors
https://www.readbyqxmd.com/read/28405940/assessing-the-interplay-between-the-physicochemical-parameters-of-ion-pairing-reagents-and-the-analyte-sequence-on-the-electrospray-desorption-process-for-oligonucleotides
#5
Babak Basiri, Mandi M Murph, Michael G Bartlett
Alkylamines are widely used as ion-pairing agents during LC-MS of oligonucleotides. In addition to a better chromatographic separation, they also assist with the desorption of oligonucleotide ions into the gas phase, cause charge state reduction, and decrease cation adduction. However, the choice of such ion-pairing agents has considerable influence on the MS signal intensity of oligonucleotides as they can also cause significant ion suppression. Interestingly, optimal ion-pairing agents should be selected on a case by case basis as their choice is strongly influenced by the sequence of the oligonucleotide under investigation...
April 12, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28402627/strategy-for-the-quantitation-of-a-protein-conjugate-via-hybrid-immunocapture-liquid-chromatography-with-sequential-hrms-and-srm-based-lc-ms-ms-analyses
#6
Yue Zhao, Guowen Liu, Xiling Yuan, Jinping Gan, Jon E Peterson, Jim X Shen
With the development of modern instrumentation and technologies, mass spectrometry based assays have played an important role in protein bioanalysis. We have developed a novel strategy by combining the "bottom-up" and "top-down" approaches using both high-resolution (HRMS) and selected reaction monitoring (SRM) based mass spectrometric detection to quantify a positron emission tomography (PET) detection tracer for an oncology marker. Monkey plasma samples were processed by immunocapture purification, followed by liquid chromatography (LC) with HRMS full scan analysis...
April 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28395255/universal-ratiometric-electrochemical-biosensing-platform-based-on-mesoporous-platinum-nanocomposite-and-nicking-endonuclease-assisted-dna-walking-strategy
#7
Qinli Pu, Junlong Li, Juhui Qiu, Xuanhua Yang, Yi Li, Dan Yin, Xinyuan Zhang, Yiyi Tao, Shangchun Sheng, Guoming Xie
The occurrence and development of many complex diseases are associated with various molecules, whose contents are rarely in the early stage of the disease. Thus a universal platform for the ultrasensitive detection of multilevel biomarkers should be developed. In this study, we introduced an electrochemical biosensing system based on nicking endonuclease (Nt.BbvCI) assisted DNA walking strategy. We successfully constructed a universal signal-off-on ratiometric electrochemical biosensor for various biomolecules, including small molecules, nucleic acids, and proteins, by progressively optimizing the schematics (schemes 1, 2, and 3)...
April 1, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28384408/polymer-dots-for-photoelectrochemical-bioanalysis
#8
Yu Li, Nan Zhang, Wei-Wei Zhao, De-Chen Jiang, Jing-Juan Xu, Hong-Yuan Chen
Different from the most extensively used inorganic quantum dots (Qdots) for the current state-of-the-art photoelectrochemical (PEC) bioanalysis, this work reports the first demonstration of polymer dots (Pdots) for novel PEC bioanalysis. The semiconducting Pdots were prepared via the reprecipitation method and then immobilized onto the transparent indium tin oxide glass electrode for PEC biodetection of the model molecule l-cysteine. The experimental results revealed that the as-fabricated Pdots exhibited excellent and interesting PEC activity and good analytical performance of rapid response, high stability, wide linear range, and excellent selectivity...
April 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28375702/beyond-antibodies-as-binding-partners-the-role-of-antibody-mimetics-in-bioanalysis
#9
Xiaowen Yu, Yu-Ping Yang, Emre Dikici, Sapna K Deo, Sylvia Daunert
The emergence of novel binding proteins or antibody mimetics capable of binding to ligand analytes in a manner analogous to that of the antigen-antibody interaction has spurred increased interest in the biotechnology and bioanalytical communities. The goal is to produce antibody mimetics designed to outperform antibodies with regard to binding affinities, cellular and tumor penetration, large-scale production, and temperature and pH stability. The generation of antibody mimetics with tailored characteristics involves the identification of a naturally occurring protein scaffold as a template that binds to a desired ligand...
March 24, 2017: Annual Review of Analytical Chemistry
https://www.readbyqxmd.com/read/28374801/few-photon-color-imaging-using-energy-dispersive-superconducting-transition-edge-sensor-spectrometry
#10
Kazuki Niwa, Takayuki Numata, Kaori Hattori, Daiji Fukuda
Highly sensitive spectral imaging is increasingly being demanded in bioanalysis research and industry to obtain the maximum information possible from molecules of different colors. We introduce an application of the superconducting transition-edge sensor (TES) technique to highly sensitive spectral imaging. A TES is an energy-dispersive photodetector that can distinguish the wavelength of each incident photon. Its effective spectral range is from the visible to the infrared (IR), up to 2800 nm, which is beyond the capabilities of other photodetectors...
April 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28371720/whole-blood-microsampling-for-the-quantitation-of-estetrol-without-derivatization-by-liquid-chromatography-tandem-mass-spectrometry
#11
Gwenaël Nys, Anne Gallez, Miranda G M Kok, Gaël Cobraiville, Anne-Catherine Servais, Géraldine Piel, Christel Pequeux, Marianne Fillet
Quantitative bioanalysis and especially pharmacokinetic studies are challenging since only low volumes of biological material are available and low concentrations (ng/ml) are often expected. In this context, volumetric absorptive microsampling (VAMS) devices were developed to accurately collect 10 or 20μl of whole blood from tested subjects. In this study, we present the development and validation of ultra-high performance liquid chromatography coupled to tandem mass spectrometry method after VAMS sampling for the quantitation of estetrol (E4), a potentially new medicine for hormone replacement, contraception and osteoporosis therapies...
June 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28362102/systems-toxicology-real-world-applications-and-opportunities
#12
Thomas Hartung, Rex E FitzGerald, Paul Jennings, Gary R Mirams, Manuel C Peitsch, Amin Rostami-Hodjegan, Imran Shah, Martin F Wilks, Shana J Sturla
Systems Toxicology aims to change the basis of how adverse biological effects of xenobiotics are characterized from empirical end points to describing modes of action as adverse outcome pathways and perturbed networks. Toward this aim, Systems Toxicology entails the integration of in vitro and in vivo toxicity data with computational modeling. This evolving approach depends critically on data reliability and relevance, which in turn depends on the quality of experimental models and bioanalysis techniques used to generate toxicological data...
April 17, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28350034/spectrophotometric-analysis-at-the-single-cell-level-elucidating-dispersity-within-melanic-immortalized-cell-populations
#13
Luis Polo-Parada, Gerardo Gutiérrez-Juárez, David Cywiak, Rafael Pérez-Solano, Gary A Baker
It is widely held that the melanosome is an exemplar of the absorption features of melanin-containing cells, which are assumed to be uniform in both size and optical characteristics. In recent years, however, it has become increasingly apparent that this is a strikingly poor assumption. Indeed, melanin extracted from natural sources and synthetic melanin both show wide variability in their degree of polymerization (molecular weight) and spectroscopic characteristics. In the current study, imaging spectrophotometry performed on individual cells of immortalized melanin-producing cell lines revealed broad distributions in their sizes: 9...
March 28, 2017: Analyst
https://www.readbyqxmd.com/read/28346882/determination-of-prodrug-treosulfan-and-its-biologically-active-monoepoxide-in-rat-plasma-liver-lungs-kidneys-muscle-and-brain-by-hplc-esi-ms-ms-method
#14
Michał Romański, Anna Kasprzyk, Artur Teżyk, Agnieszka Widerowska, Czesław Żaba, Franciszek Główka
A prodrug treosulfan (TREO) is currently investigated in clinical trials for conditioning prior to hematopoietic stem cell transplantation. Bioanalysis of TREO and its active derivatives, monoepoxide (S,S-EBDM) and diepoxide, in plasma and urine underlay the pharmacokinetic studies of these compounds but cannot explain an organ pharmacological action or toxicity. Recently, distribution of TREO and S,S-EBDM into brain, cerebrospinal fluid, and aqueous humor of the eye has been investigated in animal models and the obtained results presented clinical relevance...
June 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28343420/portable-devices-and-mobile-instruments-for-infectious-diseases-point-of-care-testing
#15
Luc Bissonnette, Michel G Bergeron
Rapidity, simplicity, and portability are highly desirable characteristics of tests and devices designed for performing diagnostics at the point of care (POC), either near patients managed in healthcare facilities or to offer bioanalytical alternatives in external settings. By reducing the turnaround time of the diagnostic cycle, POC diagnostics can reduce the dissemination, morbidity, and mortality of infectious diseases and provide tools to control the global threat of antimicrobial resistance. Areas covered: A literature search of PubMed and Google Scholar, and extensive mining of specialized publications, Internet resources, and manufacturers' websites have been used to organize and write this overview of the challenges and requirements associated with the development of portable sample-to-answer diagnostics, and showcase relevant examples of handheld devices, portable instruments, and less mobile systems which may or could be operated at POC...
April 11, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28343347/bioanalysis-of-farnesyl-pyrophosphate-in-human-plasma-by-high-performance-liquid-chromatography-coupled-to-triple-quadrupole-tandem-mass-spectrometry-and-hybrid-quadrupole-orbitrap-high-resolution-mass-spectrometry
#16
Hiroshi Sugimoto, Mie Iguchi, Fumihiro Jinno
The isoprenoids farnesyl pyrophosphate (FPP) and geranylgeranyl pyrophosphate (GGPP) are pivotal intermediates for cholesterol homeostasis and cell signaling in the mevalonate pathway. We developed a sensitive and selective high-performance liquid chromatography tandem triple quadrupole mass spectrometry (LC-QQQ-MS) method for FPP in human plasma without the need for a derivatization process. We optimized the sample preparation procedure to extract FPP and (13)C5-FPP (as internal standard) from sample fluids using methanol...
March 25, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28340771/a-dnazyme-sensor-based-on-target-catalyzed-hairpin-assembly-for-enzyme-free-and-non-label-single-nucleotide-polymorphism-genotyping
#17
Huiyan Xu, Qiwang Wu, Hong Shen
Single nucleotide polymorphisms (SNPs) are widely existed in human genome and associated with many diseases. Traditional PCR-based methods for SNP genotyping require protein enzyme, precise control of temperature and removal of resultant products, making the whole process labor intensive and time consuming. Although G-quadruplex DNAzyme-based assays provide many advantages over traditional approaches, the relatively low catalytic activity of DNAzyme becomes an unfavorable factor in its application process. Therefore, amplification of DNAzyme for further determination is of great desire in bioanalysis...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28340309/analytical-techniques-in-lipidomics-state-of-the-art
#18
Kamil Jurowski, Kamila Kochan, Justyna Walczak, Małgorzata Barańska, Wojciech Piekoszewski, Bogusław Buszewski
Current studies related to lipid identification and determination, or lipidomics in biological samples, are one of the most important issues in modern bioanalytical chemistry. There are many articles dedicated to specific analytical strategies used in lipidomics in various kinds of biological samples. However, in such literature, there is a lack of articles dedicated to a comprehensive review of the actual analytical methodologies used in lipidomics. The aim of this article is to characterize the lipidomics methods used in modern bioanalysis according to the methodological point of view: (1) chromatography/separation methods, (2) spectroscopic methods and (3) mass spectrometry and also hyphenated methods...
March 24, 2017: Critical Reviews in Analytical Chemistry
https://www.readbyqxmd.com/read/28339299/the-10th-gcc-closed-forum-rejected-data-gcp-in-bioanalysis-extract-stability-bav-processed-batch-acceptance-matrix-stability-critical-reagents-eln-and-data-integrity-and-counteracting-fraud
#19
Stephanie Cape, Rafiq Islam, Corey Nehls, John Allinson, Afshin Safavi, Patrick Bennett, James Hulse, Chris Beaver, Masood Khan, Shane Karnik, Maria Cruz Caturla, Steve Lowes, Adriana Iordachescu, Luigi Silvestro, Rabab Tayyem, Ron Shoup, Stephanie Mowery, Anahita Keyhani, Andrea Wakefield, Yinghe Li, Jennifer Zimmer, Javier Torres, Philippe Couerbe, Ardeshir Khadang, James Bourdage, Nicola Hughes, Kayode Awaiye, Brent Matthews, Saadya Fatmi, Rhonda Johnson, Christina Satterwhite, Mathilde Yu, Jenny Lin, Laura Cojocaru, Michele Fiscella, Eric Thomas, Kai Kurylak, John Kamerud, Zhongping John Lin, Wei Garofolo, Natasha Savoie, Mike Buonarati, Nadine Boudreau, Clark Williard, Yansheng Liu, Dominic Warrino, Prashant Kale, Neil Adcock, Radha Shekar, Edward O'Connor, Hanna Ritzen, Christina Sanchez, Roger Hayes, Mohammed Bouhajib, Simona Rizea Savu, Bruce Stouffer, Edward Tabler, Jing Tu, Chad Briscoe, Barry van der Strate, Paul Rhyne, Phyllis Conliffe, Ira DuBey, Jim Yamashita, Daniel Tang, Elizabeth Groeber, Jenifer Vija, Michele Malone, Mohamed Osman
The 10th Global CRO Council (GCC) Closed Forum was held in Orlando, FL, USA on 18 April 2016. In attendance were decision makers from international CRO member companies offering bioanalytical services. The objective of this meeting was for GCC members to meet and discuss scientific and regulatory issues specific to bioanalysis. The issues discussed at this closed forum included reporting data from failed method validation runs, GCP for clinical sample bioanalysis, extracted sample stability, biomarker assay validation, processed batch acceptance criteria, electronic laboratory notebooks and data integrity, Health Canada's Notice regarding replicates in matrix stability evaluations, critical reagents and regulatory approaches to counteract fraud...
March 24, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28335973/papain-functionalized-gold-nanoparticles-as-heterogeneous-biocatalyst-for-bioanalysis-and-biopharmaceuticals-analysis
#20
Siyao Liu, Markus Höldrich, Adrian Sievers-Engler, Jeannie Horak, Michael Lämmerhofer
Surface-modified gold nanoparticles (GNPs) were synthesized via layer-by-layer process with alternating cationic polyallylamine and anionic poly(acrylic acid) polyelectrolyte layers leading to a highly hydrophilic biocompatible shell supporting colloidal stability. Afterwards, papain was covalently immobilized on the modified GNPs via amide coupling between the amino groups on papain and the terminal carboxylic groups of the modified GNPs by using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide and N-hydroxysulfosuccinimide sodium as coupling agents...
April 22, 2017: Analytica Chimica Acta
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