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A P Dabkowska, C Hirst, M Valldeperas, L A Clifton, C Montis, S Nöjd, L Gentile, M Wang, G K Pálsson, S Lages, D Berti, J Barauskas, T Nylander
Polymer nanogels are embedded within layers consisting of a nonlamellar liquid crystalline lipid phase to act as thermoresponsive controllers of layer compactness and hydration. As the nanogels change from the swollen to the collapsed state via a temperature trigger, they enable on-demand release of water from the mixed polymer-lipid layer while the lipid matrix remains intact. Combining stimuli-responsive polymers with responsive lipid-based mesophase systems opens up new routes in biomedical applications such as functional biomaterials, bioanalysis and drug delivery...
January 12, 2017: Chemical Communications: Chem Comm
Tatian Kirresh, Sony Tuteja, D Russo, P D Brophy, D J Murry
A sensitive and robust LC-MS/MS method has been developed and validated to determine the concentrations of tacrolimus and its major metabolite 13-O-desmethyl tacrolimus (13-ODMT) in kidney tissue from rats who received tacrolimus intra-peritoneally at doses of 0.5mg/kg and 2mg/kg. The samples were prepared by a liquid-liquid extraction procedure using ethyl ether as the extraction solvent and ascomycin as the internal standard. Chromatographic separation was achieved using Phenomenex Kinetex column (2.6μm C18 100Å, 100×2...
December 29, 2016: Journal of Pharmaceutical and Biomedical Analysis
Tobias Schulze, Marijan Ahel, Jörg Ahlheim, Selim Aït-Aïssa, François Brion, Carolina Di Paolo, Jean Froment, Anita O Hidasi, Juliane Hollender, Henner Hollert, Meng Hu, Anett Kloß, Sanja Koprivica, Martin Krauss, Melis Muz, Peter Oswald, Margit Petre, Jennifer E Schollée, Thomas-Benjamin Seiler, Ying Shao, Jaroslav Slobodnik, Manoj Sonavane, Marc J-F Suter, Knut Erik Tollefsen, Zuzana Tousova, Karl-Heinz Walz, Werner Brack
The implementation of targeted and nontargeted chemical screening analysis in combination with in vitro and organism-level bioassays is a prerequisite for a more holistic monitoring of water quality in the future. For chemical analysis, little or no sample enrichment is often sufficient, while bioanalysis often requires larger sample volumes at a certain enrichment factor for conducting comprehensive bioassays on different endpoints or further effect-directed analysis (EDA). To avoid logistic and technical issues related to the storage and transport of large volumes of water, sampling would benefit greatly from onsite extraction...
January 3, 2017: Science of the Total Environment
Qiongdi Zhang, Ming Zhang, Lyas Deghlaf, Jeanne Bataille, Jean Gamby, Anne-Marie Haghiri-Gosnet, Antoine Pallandre
Microfluidics has emerged following the quest for scale reduction inherent to micro and nanotechnologies. By definition, microfluidics manipulates fluids in small channels with dimensions of tens to hundreds of micrometers. Recently, microfluidics has been greatly developed and its influence extends not only the domains of chemical synthesis, bioanalysis and medical researches but also optics and information technology. In this review article, we will shortly discuss an enlightening analogy between electrons transport in electronics and fluids transport in microfluidic channels...
January 6, 2017: Electrophoresis
Weiwei He, Xiangna Han, Huimin Jia, Junhui Cai, Yunlong Zhou, Zhi Zheng
Tuning the enzyme-like activity and studying the interaction between biologically relevant species and nano-enzymes may facilitate the applications of nanostructures in mimicking natural enzymes. In this work, AuPt alloy nanoparticles (NPs) with varying compositions were prepared through a facile method by co-reduction of Au(3+) and Pt(2+) in aqueous solutions. The composition could be tuned easily by adjusting the molar ratios of added Pt(2+) to Au(3+). It was found that both peroxidase-like and oxidase-like activity of AuPt alloy NPs were highly dependent on the alloy compositions, which thus suggesting an effective way to tailor their catalytic properties...
January 4, 2017: Scientific Reports
Chang Feng, Bing Bo, Xiaoxia Mao, Hai Shi, Xiaoli Zhu, Genxi Li
Both the 3D solution and the 2D interface play important roles in bioanalysis. For the former, reactions can be carried out adequately; while for the latter, interfering substance can be eliminated simply through wash. It is a challenge to integrate the advantages of solution-based assays and the interface-based assays. Here, we report an immuno-NRCA (netlike rolling circle amplification) strategy, which integrates immunoassay with NRCA for the ultrasensitive detection of tumor biomarker, by taking the assay of a tumour marker as an example...
2017: Theranostics
Yuan-Cheng Zhu, Fei Xu, Nan Zhang, Wei-Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen
This work reports the use of compositionally heterogeneous asymmetric Ag@Au core-satellite nanoassembly functionalized with DNA sequence as unique signaling nanoprobes for the realization of new energy-transfer-based photoelectrochemical (PEC) immunoassay of prostate- specific antigen (PSA). Specifically, the Ag@Au asymmetric core-satellite nanoassemblies (Ag@Au ACS) were fabricated on a two-dimensional glass substrate by a modified controlled assembly technique, and then functionalized with DNA sequences containing PSA aptamers as signaling nanoprobes...
December 20, 2016: Biosensors & Bioelectronics
W Russ Algar, Ani Khachatrian, Joseph S Melinger, Alan L Huston, Michael H Stewart, Kimihiro Susumu, Juan B Blanco-Canosa, Eunkeu Oh, Philip E Dawson, Igor L Medintz
An emerging trend with semiconductor quantum dots (QDs) is their use as scaffolds to assemble multiple energy transfer pathways. Examples to date have combined various competitive and sequential Förster resonance energy transfer (FRET) pathways between QDs and fluorescent dyes, luminescent lanthanide complexes, and bioluminescent proteins. Here, we show that the photoluminescence (PL) of QD bioconjugates can also be modulated by a combination of FRET and charge transfer (CT), and characterize the concurrent effects of these mechanistically different pathways using PL measurements at both the ensemble and the single particle level...
December 23, 2016: Journal of the American Chemical Society
Samir Messaoudi, Eric Defrancq
New applications of Pd-catalyzed coupling reactions for post-conjugation of nucleic acids have been developed recently. Breakthroughs in this area might now pave the way for the development of sophisticated DNA-probes, which can find a great interest in chemical biology, nanotechnology, bioanalysis as well as diagnostic domains.
December 21, 2016: Chembiochem: a European Journal of Chemical Biology
Hauh-Jyun Candy Chen, Chih-Huang Fan, Ya-Fen Yang
Dried blood spot (DBS) is an emerging microsampling technique for the bioanalysis of small molecules, including fatty acids, metabolites, drugs, and toxicants. DBS offers many advantages as a sample format including easy sample collection and cheap sample shipment. Hemoglobin adducts have been recognized as a suitable biomarker for monitoring chemical exposure. We previously reported that certain modified peptides in hemoglobin derived from reactive chlorine, nitrogen, and oxygen species are associated with factors including smoking, diabetes mellitus, and aging...
December 19, 2016: Chemical Research in Toxicology
Long Yuan, Li Ma, Lisa Dillon, R Marcus Fancher, Huadong Sun, Mingshe Zhu, Lois Lehman-McKeeman, Anne-Françoise Aubry, Qin C Ji
LC-MS/MS has been widely applied to the quantitative analysis of tissue samples. However, one key remaining issue is that the extraction recovery of analyte from spiked tissue calibration standard and quality control samples (QCs) may not accurately represent the "true" recovery of analyte from incurred tissue samples. This may affect the accuracy of LC-MS/MS tissue bioanalysis. Here, we investigated whether the recovery determined using tissue QCs by LC-MS/MS can accurately represent the "true" recovery from incurred tissue samples using two model compounds: BMS-986104, a S1P1 receptor modulator drug candidate, and its phosphate metabolite, BMS-986104-P...
November 16, 2016: Analytica Chimica Acta
Xinyu Wang, Jing-Lin Gao, Chaohui Du, Jing An, MengJiao Li, Haiyan Ma, Lina Zhang, Ye Jiang
AIM: People today have a stronger interest in the risk of biosafety in clinical bioanalysis. A safe, simple, effective method of preparation is needed urgently. METHODOLOGY/RESULTS: To improve biosafety of clinical analysis, we used antiviral drugs of adefovir and tenofovir as model drugs and developed a safe pretreatment method combining sealing technique with direct injection technique. The inter- and intraday precision (RSD %) of the method were <4%, and the extraction recoveries ranged from 99...
January 2017: Bioanalysis
Daniel Tang, Dafang Zhong, Kelly Dong
The fourth China Bioanalysis Forum annual conference, co-organized with the Nanjing International Drug Metabolism Conference, was successfully held in Nanjing, China, between 24-26 June 2016. The theme of the conference was 'how to conduct regulated bioanalysis under China Food and Drug Administration (CFDA) regulations'. In addition, several hot topics including bioanalytical challenges, solutions for biomarkers, antidrug antibody (antibody-drug conjugates) and biologics were discussed. This Conference Report summarizes the major discussion topics from the conference...
January 2017: Bioanalysis
Yuan-Qing Xia, Eugene Ciccimaro, Naiyu Zheng, Mingshe Zhu
AIM: It is challenging to develop a multiple reaction monitoring (MRM) method for some disulfide-bonded peptides with inefficient collision-induced dissociation fragmentation. This study describes a new methodology using differential mobility spectrometry (DMS) combined with multiple ion monitoring (MIM) to enhance bioanalytical sensitivity for sunflower trypsin inhibitor. RESULTS: By combining DMS with MIM to monitor the intact precursor ion in Q1 and Q3 MS analyzers, a lower limit of quantitation at 0...
January 2017: Bioanalysis
Sankeetha Nadarajah
Welcome to volume 9 of Bioanalysis and Happy New Year to all of our readers. We would like to take the opportunity to look back 2016, which was another great year for us. We thank all our authors, readers and reviewers, as well as our Editorial Board members for their continued support. We very much look forward to working with everyone in 2017.
January 2017: Bioanalysis
Giovanni Valenti, Enrico Rampazzo, Sara Bonacchi, Luca Petrizza, Massimo Marcaccio, Marco Montalti, Luca Prodi, Francesco Paolucci
The impact of nanotechnology on analytical science is hardly overlooked. In the search for ever-increasing sensitivity in biomedical sensors, nanoparticles have been playing a unique role as, for instance, ultrabright labels, and unravelling the intimate mechanisms which govern their functioning is mandatory for the design of ultrasentitive devices. Herein, we investigated the mechanism of electrogenerated chemiluminescence (ECL) in a family of core-shell silica-PEG nanoparticles (DDSNs), variously doped with a Ru(bpy)3(2+) triethoxysilane derivative, and displaying homogeneous morphological, hydrodynamic, and photophysical properties...
December 14, 2016: Journal of the American Chemical Society
Kanumuri Siva Rama Raju, Manoj Gundeti, Mohd Yaseen Malik, Naveen Kadian, Mamunur Rashid, Isha Taneja, Sheelendra Pratap Singh, Muhammad Wahajuddin
Tuberculosis is a life threatening disease and second to HIV in terms of deaths due to infectious diseases. Drug resistance development of the first-line drugs is a major concern in the treatment of this disease. There is no comprehensive and critical review in the literature of the bioanalytical methods for the determination of anti-tubercular agents from last two decades. This work offers a detailed account on the liquid chromatographic methods reported in the literature for the estimation of various anti-tubercular drugs...
February 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
Alessia Stornetta, Maike Zimmermann, George D Cimino, Paul T Henderson, Shana J Sturla
Biomarker-driven drug selection plays a central role in cancer drug discovery and development, and in diagnostic strategies to improve the use of traditional chemotherapeutic drugs. DNA-modifying anticancer drugs are still used as first line medication, but drawbacks such as resistance and side effects remain an issue. Monitoring the formation and level of DNA modifications induced by anticancer drugs is a potential strategy for stratifying patients and predicting drug efficacy. In this perspective, preclinical and clinical data concerning the relationship between drug-induced DNA adducts and biological response for platinum drugs and combination therapies, nitrogen mustards and half-mustards, hypoxia-activated drugs, reductase-activated drugs, and minor groove binding agents are presented and discussed...
January 3, 2017: Chemical Research in Toxicology
Heidi Toft Hansen, Christian Janfelt
Internal standards have been introduced in quantitative mass spectrometry imaging in order to compensate for differences in intensities throughout an image caused by, for example, difference in ion suppression or analyte extraction efficiency. To test how well the internal standards compensate for differences in tissue types in, for example, whole-body imaging, a set of tissue homogenates of different tissue types (lung, liver, kidney, heart, and brain) from rabbit was spiked to the same concentration with the drug amitriptyline and imaged in the same experiment using isotope labeled amitriptyline as internal standard...
December 6, 2016: Analytical Chemistry
Jiali Liu, Min Zhao, Ying Zhuo, Yaqin Chai, Ruo Yuan
The intermolecular electrochemiluminescence resonance energy transfer (ECL-RET) between luminol and Ru(bpy)32+ was studied extensively to achieve the sensitive bio-analysis owing to the perfect spectral overlap of the donor and acceptor, but it still suffers from the challenging issue of low energy transfer efficiency. Here, the intramolecular ECL-RET towards the novel ECL compound containing the donor of luminol and the acceptor of Ru(bpy)2(mcbpy)2+ was designed and investigated for the first time (The compound was abbreviated as Lum-Ru)...
December 8, 2016: Chemistry: a European Journal
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