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https://www.readbyqxmd.com/read/28622470/patterned-photonic-nitrocellulose-for-pseudo-paper-elisa
#1
Junjie Chi, Bingbing Gao, Mi Sun, Fengling Zhang, Enben Su, Hong Liu, Zhongze Gu
We report an enzyme-link immunosorbent assay (ELISA) based on patterned pseudo-paper that is made of photonic nitrocellulose for highly sensitive fluorescent bioanalysis. The pseudo-paper is fabricated using self-assembled monodisperse SiO2 nanoparticles that are patterned on a polypropylene substrate as template. The self-assembled nanoparticles have a close-packed hexagonal (opal) structure, so the resulting nitrocellulose has a complementary (inverse opal) photonic structure. Owing to the slow-photon effect of the photonic structure, fluorescent emission for ELISA is enhanced by up to 57-fold without increasing the assay time or complexity...
June 16, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28621518/a-photoelectrochemical-bioanalysis-platform-for-cells-monitoring-based-on-dual-signal-amplification-using-in-situ-generation-of-electron-acceptor-coupled-with-heterojunction
#2
Ruyan Li, Yue Zhang, Wenwen Tu, Zhihui Dai
Using in suit generation of electron acceptor coupled with heterojunction as dual signal amplification, a simple photoelectrochemical (PEC) bioanalysis platform was designed. The synergic effect between the PEC activities of carbon nitride (C3N4) nanosheets and PbS quantum dots (QDs) achieved almost 9 fold photocurrent intensity increment, compared with the C3N4 alone. After the G-quadruplex/hemin/Pt NPs with catalase-like activity towards H2O2 was introduced, oxygen was in situ generated and acted as electron donor, improving charge separation efficiency and further enhancing photocurrent response...
June 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28618051/quantitative-bioanalysis-of-bavachalcone-in-rat-plasma-by-lc-ms-ms-and-its-application-in-a-pharmacokinetics-study
#3
Dan Zhou, Lianhua An, Yan Xia, Yuanyi Wang, Xingliang Li
This study aims to develop and validate a simple and sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for investigating the pharmacokinetic characteristics of bavachalcone. Liquid-liquid extraction was used to prepare plasma sample. Chromatographic separation of bavachalcone and IS was achieved by using a Venusil ASB C18 (2.1 × 50 mm, 5 μm) column with a mobile phase of methanol (A)-water (B) (70:30, v/v). The detection and quantification of analytes was performed in selected-reaction monitoring mode using precursor→product ion combinations of m/z 323...
June 15, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28617495/thiol-ene-micropillar-array-electrospray-ionization-platform-for-zeptomole-level-bioanalysis
#4
K Lipponen, S Tähkä, T Sikanen, V Jokinen, A Tatikonda, S Franssila, R Kostiainen, T Kotiaho
A micropillar array electrospray ionization (μPESI) platform fabricated from thiol-enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample. The thiol-ene polyethylene glycol (PEG) coated μPESI chip allows the use of a wide range of aqueous-organic solvent compositions and provides a detection limit at 60 zeptomole level (6 × 10(-20) mol) for a peptide standard...
June 15, 2017: Analyst
https://www.readbyqxmd.com/read/28612085/moving-from-millifluidic-to-truly-microfluidic-sub-100-%C3%AE-m-cross-section-3d-printed-devices
#5
Michael J Beauchamp, Gregory P Nordin, Adam T Woolley
Three-dimensional (3D) printing has generated considerable excitement in recent years regarding the extensive possibilities of this enabling technology. One area in which 3D printing has potential, not only for positive impact but also for substantial improvement, is microfluidics. To date many researchers have used 3D printers to make fluidic channels directed at point-of-care or lab-on-a-chip applications. Here, we look critically at the cross-sectional sizes of these 3D printed fluidic structures, classifying them as millifluidic (larger than 1 mm), sub-millifluidic (0...
July 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28602189/one-step-electro-oxidative-preparation-of-graphene-quantum-dots-from-wood-charcoal-as-a-peroxidase-mimetic
#6
Narsingh R Nirala, Gaurav Khandelwal, Brijesh Kumar, Vinita, Rajiv Prakash, Vinod Kumar
In present study, we highlight one-step electrochemical synthesis of nearly uniform size (~ 5nm) of graphene quantum dots (E-GQDs) from wood charcoal and their further application as a peroxidase enzyme mimetic. The structural and optical properties of as-synthesized E-GQDs were probed by TEM, AFM, and spectroscopic techniques. Peroxidase enzyme mimetic potential of E-GQDs were examined for colorimetric detection of H2O2 and glucose. E-GQDs allowed a rapid and sensitive detection of glucose with a detection limit of 0...
October 1, 2017: Talanta
https://www.readbyqxmd.com/read/28601765/application-of-volumetric-absorptive-microsampling-device-for-quantification-of-tacrolimus-in-human-blood-as-a-model-drug-of-high-blood-cell-partition
#7
Kenji Kita, Yuji Mano
Volumetric absorptive microsampling device (VAMS) was evaluated for bioanalysis of tacrolimus, which was used as a model drug with high blood cell partition. Aliquots of blood (ca. 10μL) with different hematocrits and fortified with tacrolimus were wicked up by VAMS and tacrolimus was extracted with a methanol-water mixture (1:1, v/v) via sonication. After chromatography on an AQUITY UPLC HSS T3 column (100×2.1 i.d., mm, 1.8μm), tacrolimus and the internal standard ascomycin, were detected in the positive ion mode with electrospray ionization by monitoring of transitions m/z 826...
June 3, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28593761/enediol-ligands-encapsulated-liposomes-enables-sensitive-immunoassay-a-proof-of-concept-for-general-liposomes-based-photoelectrochemical-bioanalysis
#8
Li-Ping Mei, Fei Liu, Jian-Bin Pan, Wei-Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen
Sensitive photoelectrochemical (PEC) bioanalysis usually relies on enzyme-assisted signal amplification. This work describes the first proof-of-concept study for liposome-based PEC bioanalysis. Specifically, unilamellar liposomes were prepared and then utilized to carry the enediol-ligands and antibodies within their internal cavities and upon their external surfaces, respectively. On the other hand, the 96-well plate was used for accommodating the sandwich immunocomplexing, and then the confined liposomes were directed to release the encapsulated enediol-ligands into an individual well...
June 9, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28576492/a-dna-sequence-obtained-by-replacement-of-the-dopamine-rna-aptamer-bases-is-not-an-aptamer
#9
Isabel Álvarez-Martos, Elena E Ferapontova
A unique specificity of the aptamer-ligand biorecognition and binding facilitates bioanalysis and biosensor development, contributing to discrimination of structurally related molecules, such as dopamine and other catecholamine neurotransmitters. The aptamer sequence capable of specific binding of dopamine is a 57 nucleotides long RNA sequence reported in 1997 (Biochemistry, 1997, 36, 9726). Later, it was suggested that the DNA homologue of the RNA aptamer retains the specificity of dopamine binding (Biochem...
May 31, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28573275/hybridization-chain-reaction-a-versatile-molecular-tool-for-biosensing-bioimaging-and-biomedicine
#10
REVIEW
Sai Bi, Shuzhen Yue, Shusheng Zhang
Developing powerful, simple and low-cost DNA amplification techniques is of great significance to bioanalysis and biomedical research. Thus far, many signal amplification strategies have been developed, such as polymerase chain reaction (PCR), rolling circle amplification (RCA), and DNA strand displacement amplification (SDA). In particular, hybridization chain reaction (HCR), a type of toehold-mediated strand displacement (TMSD) reaction, has attracted great interest because of its enzyme-free nature, isothermal conditions, simple protocols, and excellent amplification efficiency...
June 2, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28560668/aptamer-based-methods-for-detection-of-circulating-tumor-cells-and-their-potential-for-personalized-diagnostics
#11
Anna S Zamay, Galina S Zamay, Olga S Kolovskaya, Tatiana N Zamay, Maxim V Berezovski
Cancer diagnostics and treatment monitoring rely on sensing and counting of rare cells such as cancer circulating tumor cells (CTCs) in blood. Many analytical techniques have been developed to reliably detect and quantify CTCs using unique physical shape and size of tumor cells and/or distinctive patterns of cell surface biomarkers. Main problems of CTC bioanalysis are in the small number of cells that are present in the circulation and heterogeneity of CTCs. In this chapter, we describe recent progress towards the selection and application of synthetic DNA or RNA aptamers to capture and detect CTCs in blood...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28528664/enhanced-lc-ms-ms-analysis-of-alogliptin-and-pioglitazone-in-human-plasma-applied-to-a-preliminary-pharmacokinetic-study
#12
Maha F Abdel-Ghany, Miriam F Ayad, Mariam M Tadros
A new fast LC-MS/MS method was developed for determination of alogliptin and pioglitazone in human plasma. Linearity ranges of 10-400ngmL(-1) for alogliptin and 25-2000ngmL(-1) for pioglitazone, were found to be suitable for their bioanalysis covering the Cmin and Cmax values of the drugs. Direct precipitation technique was used for simultaneous extraction of the drugs successfully from human plasma samples. Chromatographic separation was achieved on a BEH C18 column (50mm×2.1mm, 1.7μm) with 0.1% aqueous formic acid: acetonitrile (40:60, v/v) at a flow rate of 0...
July 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28525851/a-fully-integrated-distance-readout-elisa-chip-for-point-of-care-testing-with-sample-in-answer-out-capability
#13
Dan Liu, Xingrui Li, Junkai Zhou, Shibo Liu, Tian Tian, Yanling Song, Zhi Zhu, Leiji Zhou, Tianhai Ji, Chaoyong Yang
Enzyme-linked immunosorbent assay (ELISA) is a popular laboratory technique for detection of disease-specific protein biomarkers with high specificity and sensitivity. However, ELISA requires labor-intensive and time-consuming procedures with skilled operators and spectroscopic instrumentation. Simplification of the procedures and miniaturization of the devices are crucial for ELISA-based point-of-care (POC) testing in resource-limited settings. Here, we present a fully integrated, instrument-free, low-cost and portable POC platform which integrates the process of ELISA and the distance readout into a single microfluidic chip...
May 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28524734/capillary-microsampling-in-nonclinical-safety-assessment-practical-sampling-and-bioanalysis-from-a-cro-perspective
#14
David Coleman, Graeme Smith, Rachel Lawrence, Deborah McManus, Sunetha Diaram, Joanna Edwards
Microsampling has the 3R benefits of refining blood collection techniques while reducing the number of animals required for rodent safety assessment studies. There are significant scientific benefits of correlating study findings with systemic exposure and consequently, there is an industry drive to utilize microsampling in regulatory toxicology studies. This article will focus on capillary microsampling and will discuss the initial considerations before using capillary microsampling, study design and some practicalities of sample collection...
May 19, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28521276/an-atmospheric-pressure-ionization-source-using-a-high-voltage-target-compared-to-electrospray-ionization-for-the-lc-ms-analysis-of-pharmaceutical-compounds
#15
Arnaud Lubin, Ronald De Vries, Deirdre Cabooter, Patrick Augustijns, Filip Cuyckens
The type and design of an ionization source can have a significant influence on the performances of a bioanalytical method. It is, therefore, of high interest to evaluate the performances of newly introduced sources to highlight their benefits and limitations in comparison to other well established sources. In this paper, liquid chromatography - mass spectrometry (LC/MS) performances of a new atmospheric pressure ionization (API) source, commercialized as UniSpray, is evaluated. The dynamic range of 24 pharmaceutical and biological compounds is compared between the new API source and electrospray ionization (ESI) for 3 different mobile phase conditions...
May 4, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28520462/activity-based-proteomics-in-bioanalysis-past-present-and-future
#16
Rituraj Dubey, Ravi Bhushan
No abstract text is available yet for this article.
May 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28520459/feedback-from-the-ebf-focus-workshop-bringing-assay-validation-and-analysis-of-biomarkers-into-practice
#17
Philip Timmerman, Marianne Scheel Fjording, John Allinson, Cecilia Arfvidsson, Begoña Barroso, Ulf Diczfalusy, Adrian Freeman, Elizabeth Hickford, Hamza Kandousi, Sidath Katugampola, Ulrich Kunz, Robert Nelson, John Smeraglia
European Bioanalysis Forum, Lisbon, Portugal, 9-10 June 2016 At the recent European Bioanalysis Forum's Focus Workshop 'Bringing Assay Validation and Analysis of Biomarkers into Practice', the discussion on best practice for biomarker assay validation continued. Both the presentations and the adjacent panel discussions yielded valuable food for thought for the broader bioanalytical community. The present conference report summarizes the essence from these discussions and from the proposals or conclusions made by all delegates on how to increase the necessary connectivity of the stakeholders involved in the bioanalysis of biomarkers...
May 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28520455/quantitative-determination-of-avi-7100-radavirsen-a-phosphorodiamidate-morpholino-oligomer-pmoplus-%C3%A2-in-human-plasma-using-lc-ms-ms
#18
Min Meng, Jianbo Zhang, Aihua Liu, Scott Reuschel, Pete Sazani, Michael Wong
AIM: AVI-7100 (Radavirsen) is a 20-mer phosphorodiamidate morpholino oligomer (PMOplus®) for the treatment of influenza. Results/methodology: An automated solid-phase extraction method was used to extract plasma samples (200 μl). The extracts were analyzed using liquid chromatography coupled with tandem mass spectrometry under the positive ionization mode. This method was fully validated over the calibration curve range of 5.00-1000 ng/ml. The between-run precision and accuracy ranged from 0...
May 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28511118/simultaneous-determination-of-selective-serotonin-reuptake-inhibitors-and-their-main-metabolites-in-human-breast-milk-by-liquid-chromatography-electrospray-mass-spectrometry
#19
Etienne Weisskopf, Alice Panchaud, Kim An Nguyen, Delphine Grosjean, Jean-Michel Hascoët, Chantal Csajka, Chin B Eap, Nicolas Ansermot
A bioanalytical method by high performance liquid chromatography coupled to electrospray mass spectrometry (HPLC-ESI-MS), adapted from a previously published method in plasma, was validated in breast milk for the simultaneous quantification of all antidepressants belonging to the class of selective serotonin reuptake inhibitors (citalopram, fluoxetine, fluvoxamine, paroxetine and sertraline) and their major metabolites (desmethylcitalopram and norfluoxetine). Milk samples (250μl) first underwent protein precipitation followed by solid-phase extraction on a reversed phase/cation exchange sorbent...
July 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28508542/dynamic-self-assembly-of-homogenous-microcyclic-structures-controlled-by-a-silver-coated-nanopore
#20
Rui Gao, Yao Lin, Yi-Lun Ying, Xiao-Yuan Liu, Xin Shi, Yong-Xu Hu, Yi-Tao Long, He Tian
The self-assembly of nanoparticles is a challenging process for organizing precise structures with complicated and ingenious structures. In the past decades, a simple, high-efficiency, and reproducible self-assembly method from nanoscale to microscale has been pursued because of the promising and extensive application prospects in bioanalysis, catalysis, photonics, and energy storage. However, microscale self-assembly still faces big challenges including improving the stability and homogeneity as well as pursuing new assembly methods and templates for the uniform self-assembly...
May 16, 2017: Small
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