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https://www.readbyqxmd.com/read/27908259/a-novel-liquid-chromatography-tandem-mass-spectrometry-method-for-the-estimation-of-bilirubin-glucuronides-and-its-application-to-in-vitro-enzyme-assays
#1
Siva Prasad Putluru, Murali Krishna Matta, Deepak Ahire, Murali Subramanian, Michael Sinz, Sandhya Mandlekar
Bilirubin is a toxic waste product of metabolism, eliminated mainly through UGT1A1 mediated conjugation to mono- and di-glucuronides. Due to the potentially low Km value of bilirubin glucuronidation, the quantitative sensitivity obtained with most UV/visible light detection methods are not sufficient to accurately calculate UGT1A1 enzyme kinetics and inhibition properties of new chemical entities at low bilirubin concentrations. In addition, bilirubin, as well as its metabolites, are unstable during sample preparation and bioanalysis...
November 24, 2016: Drug Metabolism Letters
https://www.readbyqxmd.com/read/27907869/is-a-deuterated-internal-standard-appropriate-for-the-reliable-determination-of-olmesartan-in-human-plasma
#2
Edyta Piórkowska, Jacek Musijowski, Katarzyna Buś-Kwaśnik, Piotr J Rudzki
A right choice of the internal standard is one of the most challenging tasks during bioanalytical method development. Surprisingly, among the HPLC-MS methods for the determination of a cardiovascular drug olmesartan in plasma only structural analogues or similar compounds were used as internal standards. We have tried to answer the question whether the stable isotope labelled (deuterated) internal standard, as recommended by regulatory agencies, can be used for the reliable determination of olmesartan in human plasma...
November 16, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27904944/isothermal-nucleic-acid-amplification-in-bioanalysis
#3
EDITORIAL
María Jesús Lobo-Castañón
No abstract text is available yet for this article.
December 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27900235/technical-challenges-in-mass-spectrometry-based-metabolomics
#4
Fumio Matsuda
Metabolomics is a strategy for analysis, and quantification of the complete collection of metabolites present in biological samples. Metabolomics is an emerging area of scientific research because there are many application areas including clinical, agricultural, and medical researches for the biomarker discovery and the metabolic system analysis by employing widely targeted analysis of a few hundred preselected metabolites from 10-100 biological samples. Further improvement in technologies of mass spectrometry in terms of experimental design for larger scale analysis, computational methods for tandem mass spectrometry-based elucidation of metabolites, and specific instrumentation for advanced bioanalysis will enable more comprehensive metabolome analysis for exploring the hidden secrets of metabolism...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/27884077/from-patient-to-tube-the-importance-of-physiologically-relevant-quantitative-bioanalytical-assays
#5
Scott Summerfield, Matthew Barfield, Neil Spooner, Steve White
Circulating drug concentrations (clinical or preclinical) underly many interactions between industry and regulators; expressing safety coverage, pharmacokinetic-pharmacodynamic relationships or defining bioequivalence and dosing regimens. Accurate and precise measurement of these circulating concentrations is pivotal to the evolution and validation of any bioanalytical method that supports regulatory interactions. Since the bioanalyst is presented with a sub-aliquot of sampled biological matrix, how do they ensure this aliquot reflects the concentration in the subject at the time of collection? Here we share experiences from project support (internal and at CROs) that suggests we need to be ever vigilant translating the needs of bioanalysis with those of project teams...
December 2016: Bioanalysis
https://www.readbyqxmd.com/read/27884073/liquid-chromatography-high-resolution-mass-spectrometry-for-regulated-bioanalysis-bile-acid-and-oligonucleotide-quantitation-as-a-reference
#6
https://www.readbyqxmd.com/read/27872920/ultrafast-chiral-separations-for-high-throughput-enantiopurity-analysis
#7
Chandan L Barhate, Leo A Joyce, Alexey A Makarov, Kerstin Zawatzky, Frank Bernardoni, Wes A Schafer, Daniel W Armstrong, Christopher J Welch, Erik L Regalado
Recent developments in fast chromatographic enantioseparations now make high throughput analysis of enantiopurity on the order of a few seconds achievable. Nevertheless, routine chromatographic determinations of enantiopurity to support stereochemical investigations in pharmaceutical research and development, synthetic chemistry and bioanalysis are still typically performed on the 5-20 min timescale, with many practitioners believing that sub-minute enantioseparations are not representative of the molecules encountered in day to day research...
November 22, 2016: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/27866844/new-materials-for-sample-preparation-techniques-in-bioanalysis
#8
REVIEW
Carlos Eduardo Domingues Nazario, Bruno Henrique Fumes, Meire Ribeiro da Silva, Fernando Mauro Lanças
The analysis of biological samples is a complex and difficult task owing to two basic and complementary issues: the high complexity of most biological matrices and the need to determine minute quantities of active substances and contaminants in such complex sample. To succeed in this endeavor samples are usually subject to three steps of a comprehensive analytical methodological approach: sample preparation, analytes isolation (usually utilizing a chromatographic technique) and qualitative/quantitative analysis (usually with the aid of mass spectrometric tools)...
November 3, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27862751/label-free-molecular-imaging-of-biological-cells-and-tissues-by-linear-and-non-linear-raman-spectroscopic-approaches
#9
Jürgen Popp, Christoph Krafft, Michael Schmitt, Iwan Schie, Dana Cialla-May, Christian Matthaeus, Thomas Bocklitz
Raman spectroscopy is an emerging technique in bioanalysis and imaging of biomaterials due to its unique spectroscopic fingerprint capabilities. Imaging of cells and tissues by Raman microspectroscopy represents a non-destructive and label-free approach. All components of cells or tissues contribute to the Raman signals giving complex spectral signatures. Long acquisitions times are often required due to the relatively small Raman scattering cross sections. To overcome these limitations, Raman signal enhancing methods like resonance Raman scattering and surface enhanced Raman scattering can be applied that also reduce the spectral complexity because the enhancement is often restricted to selected bands...
November 15, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27855512/2016-white-paper-on-recent-issues-in-bioanalysis-focus-on-biomarker-assay-validation-bav-part-3-lba-biomarkers-and-immunogenicity
#10
Susan Richards, Lakshmi Amaravadi, Renuka Pillutla, Herbert Birnboeck, Albert Torri, Kyra J Cowan, Apollon Papadimitriou, Fabio Garofolo, Christina Satterwhite, Steven Piccoli, Bonnie Wu, Corinna Krinos-Fiorotti, John Allinson, Flora Berisha, Laurent Cocea, Stephanie Croft, Stephanie Fraser, Fabrizio Galliccia, Boris Gorovits, Swati Gupta, Vinita Gupta, Sam Haidar, Charles Hottenstein, Akiko Ishii-Watabe, Darshana Jani, John Kadavil, John Kamerud, Daniel Kramer, Virginia Litwin, Gustavo Mendes Lima Santos, Robert Nelson, Yan Ni, João Pedras-Vasconcelos, Yongchang Qiu, Paul Rhyne, Afshin Safavi, Yoshiro Saito, Natasha Savoie, Kara Scheibner, Eginhard Schick, Patricia Y Siguenza, John Smeraglia, Roland F Staack, Meena Subramanyam, Giane Sumner, Theingi Thway, David Uhlinger, Martin Ullmann, Alessandra Vitaliti, Jan Welink, Chan C Whiting, Li Xue, Rong Zeng
The 2016 10th Workshop on Recent Issues in Bioanalysis (10th WRIB) took place in Orlando, Florida with participation of close to 700 professionals from pharmaceutical/biopharmaceutical companies, biotechnology companies, contract research organizations, and regulatory agencies worldwide. WRIB was once again a weeklong event - A Full Immersion Week of Bioanalysis for PK, Biomarkers and Immunogenicity. As usual, it is specifically designed to facilitate sharing, reviewing, discussing and agreeing on approaches to address the most current issues of interest including both small and large molecules involving LCMS, hybrid LBA/LCMS, and LBA approaches, with the focus on PK, biomarkers and immunogenicity...
December 2016: Bioanalysis
https://www.readbyqxmd.com/read/27855511/accuracy-a-potential-quandary-in-regulated-bioanalysis-of-endogenous-analytes
#11
Rand G Jenkins
No abstract text is available yet for this article.
December 2016: Bioanalysis
https://www.readbyqxmd.com/read/27855510/uhplc-ms-ms-bioanalysis-of-urinary-dhea-cortisone-and-their-hydroxylated-metabolites-as-potential-biomarkers-for-cyp3a-mediated-drug-drug-interactions
#12
Naiyu Zheng, Lisa J Christopher, Xuewen Ma, Qin C Ji, Adela Buzescu, Hamza Kandoussi, Samira M Garonzik, Frank LaCreta, Anne-Francoise Aubry, Mark E Arnold, Jianing Zeng
AIM: A UHPLC-MS/MS assay was developed to quantify urinary dehydroepiandrosterone (DHEA), 7β-hydroxy-DHEA, cortisone and 6β-hydroxycortisone as potential biomarkers to predict CYP3A activity. RESULTS: A sensitive assay at LLOQ of 0.500 ng/ml with good accuracy and precision was developed for the four analytes in human urine. This UHPLC-MS/MS assay was optimized by eliminating nonspecific loss of the analytes in urine, ensuring complete hydrolysis of the conjugates to unconjugated forms and use of the product ions of [M+H-H2O](+) for multiple reaction monitoring detection of DHEA and 7β-hydroxy-DHEA...
December 2016: Bioanalysis
https://www.readbyqxmd.com/read/27855509/2016-white-paper-on-recent-issues-in-bioanalysis-focus-on-biomarker-assay-validation-bav-part-2-hybrid-lba-lcms-and-input-from-regulatory-agencies
#13
An Song, Anita Lee, Fabio Garofolo, Surinder Kaur, Jeff Duggan, Christopher Evans, Joe Palandra, Lorella Di Donato, Keyang Xu, Ronald Bauer, Mark Bustard, Linzhi Chen, Laurent Cocea, Stephanie Croft, Fabrizio Galliccia, Sam Haidar, Nicola Hughes, Akiko Ishii-Watabe, Rafiqul Islam, Barry Jones, John Kadavil, Carsten Krantz, Gustavo Mendes Lima Santos, Timothy Olah, João Pedras-Vasconcelos, Ludovicus Staelens, Yoshiro Saito, Natasha Savoie, Kara Scheibner, Susan Spitz, Nilufer Tampal, Eric Thomas, Stephen Vinter, Jason Wakelin-Smith, Jan Welink, Jianing Zeng, Shaolian Zhou
The 2016 10th Workshop on Recent Issues in Bioanalysis (10(th) WRIB) took place in Orlando, Florida with participation of close to 700 professionals from pharmaceutical/biopharmaceutical companies, biotechnology companies, contract research organizations, and regulatory agencies worldwide. WRIB was once again a 5-day, weeklong event - A Full Immersion Week of Bioanalysis including Biomarkers and Immunogenicity. As usual, it is specifically designed to facilitate sharing, reviewing, discussing and agreeing on approaches to address the most current issues of interest including both small and large molecules involving LCMS, hybrid LBA/LCMS, and LBA approaches, with the focus on biomarkers and immunogenicity...
December 2016: Bioanalysis
https://www.readbyqxmd.com/read/27855505/a-simple-fast-sensitive-and-robust-lc-ms-ms-bioanalytical-assay-for-evaluating-7%C3%AE-hydroxy-4-cholesten-3-one-biomarker-in-a-clinical-program
#14
Long Yuan, Yi Luo, Hamza Kandoussi, Qin C Ji
AIM: Serum 7α-hydroxy-cholesten-3-one (C4) has been reported as a biomarker to assess CYP7A1 enzyme activity and bile acid synthesis. To support a clinical program, a sensitive and reliable assay without derivatization was required for the analysis of C4 in human serum. Methodology & results: A systematic approach was used to optimize mass spectrometry, LC and sample extraction conditions, therefore, significantly improved assay sensitivity, and achieved the required quantification limit without derivatization...
December 2016: Bioanalysis
https://www.readbyqxmd.com/read/27836609/target-triggered-dna-nanoassembly-on-quantum-dots-and-dnazyme-modulated-double-quenching-for-ultrasensitive-microrna-biosensing
#15
Rui Yuan, Xiaolin Yu, Yuhong Zhang, Lulu Xu, Wei Cheng, Zhiguang Tu, Shijia Ding
Herein, a simple and novel fluorescence biosensing strategy has been developed for ultrasensitive determination of microRNA (miRNA) by combining target-triggered DNA nanoassembly on quantum dots (QDs) with DNAzyme-modulated double quenching of QDs. In presence of miRNA target, the target triggered catalytic hairpin assembly (CHA) amplification and powered highly efficient DNA nanoassembly on the surface of QDs, leading to exhibition of numerous G-quadruplexes close to the QDs. The G-quadruplex folded properly and bound hemin to form a stable G-quadruplex/hemin complex...
November 5, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27836594/photoelectrochemical-enzymatic-biosensors
#16
REVIEW
Wei-Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen
Enzymatic biosensors have been valuable bioanalytical devices for analysis of diverse targets in disease diagnosis, biological and biomedical research, etc. Photoelectrochemical (PEC) bioanalysis is a recently emerged method that promptly becoming a subject of new research interests due to its attractive potential for future bioanalysis with high sensitivity and specificity. PEC enzymatic biosensors integrate the inherent sensitivities of PEC bioanalysis and the selectivity of enzymes and thus share their both advantages...
November 7, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27836592/a-novel-signal-on-off-sensing-platform-for-selective-detection-of-thrombin-based-on-target-induced-ratiometric-electrochemical-biosensing-and-bio-bar-coded-nanoprobe-amplification-strategy
#17
Lanlan Wang, Rongna Ma, Liushan Jiang, Liping Jia, Wenli Jia, Huaisheng Wang
A novel dual-signal ratiometric electrochemical aptasensor for highly sensitive and selective detection of thrombin has been designed on the basis of signal-on and signal-off strategy. Ferrocene labeled hairpin probe (Fc-HP), thrombin aptamer and methyl blue labeled bio-bar-coded AuNPs (MB-P3-AuNPs) were rationally introduced for the construction of the assay platform, which combined the advantages of the recognition of aptamer, the amplification of bio-bar-coded nanoprobe, and the ratiometric signaling readout...
November 1, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27810763/integrating-chemical-analysis-and-bioanalysis-to-evaluate-the-contribution-of-wastewater-effluent-on-the-micropollutant-burden-in-small-streams
#18
Peta A Neale, Nicole A Munz, Selim Aїt-Aїssa, Rolf Altenburger, François Brion, Wibke Busch, Beate I Escher, Klára Hilscherová, Cornelia Kienle, Jiří Novák, Thomas-Benjamin Seiler, Ying Shao, Christian Stamm, Juliane Hollender
Surface waters can contain a range of micropollutants from point sources, such as wastewater effluent, and diffuse sources, such as agriculture. Characterizing the source of micropollutants is important for reducing their burden and thus mitigating adverse effects on aquatic ecosystems. In this study, chemical analysis and bioanalysis were applied to assess the micropollutant burden during low flow conditions upstream and downstream of three wastewater treatment plants (WWTPs) discharging into small streams in the Swiss Plateau...
October 28, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27809492/cu-nanoclusters-novel-electrochemiluminescence-emitters-for-bioanalysis
#19
Min Zhao, Anyi Chen, Dan Huang, Ying Zhuo, Yaqin Chai, Ruo Yuan
Cu nanoclusters (Cu NCs), emerged as a new class of non-toxic, economic, and excellent phosphor and catalyst, have attracted increasing interest for a wide variety of promising applications in bio-labeling and bio-catalysis. However, the electrochemiluminescence (ECL) behavior of Cu NCs has never reported in previous works. Here, anodic and blue ECL emission of Cu NCs is observed for the first time with the efficient coreactant of hydrazine (HZ), and the possible luminescence mechanism of Cu NCs/HZ ECL system is studied in detail...
November 4, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27796911/accelerating-regulated-bioanalysis-for-biotherapeutics-case-examples-using-a-microfluidic-ligand-binding-assay-platform
#20
Rong Liu, Brian Hoffpauir, Shannon D Chilewski, Janice Gamberdella, Uma Kavita, Jia Duo, Carol Gleason, Yan Zhang, Renuka Pillutla, Binodh DeSilva, Lora Hamuro
The Gyrolab™ xP is a microfluidic platform for conducting ligand binding assays (LBAs) and is recognized for its utility in discovery bioanalysis. However, few reports have focused on the technology for regulated bioanalysis. This technology has the advantage of low reagent consumption, low sample volume, and automated ligand binding methods. To improve bioanalysis testing timelines and increase the speed at which biotherapeutics are delivered to patients, we evaluated the technology for its potential to deliver high-quality data at reduced testing timelines for regulated bioanalysis...
October 27, 2016: AAPS Journal
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