keyword
MENU ▼
Read by QxMD icon Read
search

Bioanalysis

keyword
https://www.readbyqxmd.com/read/28528664/enhanced-lc-ms-ms-analysis-of-alogliptin-and-pioglitazone-in-human-plasma-applied-to-a-preliminary-pharmacokinetic-study
#1
Maha F Abdel-Ghany, Miriam F Ayad, Mariam M Tadros
A new fast LC-MS/MS method was developed for determination of alogliptin and pioglitazone in human plasma. Linearity ranges of 10-400ngmL(-1) for alogliptin and 25-2000ngmL(-1) for pioglitazone, were found to be suitable for their bioanalysis covering the Cmin and Cmax values of the drugs. Direct precipitation technique was used for simultaneous extraction of the drugs successfully from human plasma samples. Chromatographic separation was achieved on a BEH C18 column (50mm×2.1mm, 1.7μm) with 0.1% aqueous formic acid: acetonitrile (40:60, v/v) at a flow rate of 0...
April 26, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28525851/a-fully-integrated-distance-readout-elisa-chip-for-point-of-care-testing-with-sample-in-answer-out-capability
#2
Dan Liu, Xingrui Li, Junkai Zhou, Shibo Liu, Tian Tian, Yanling Song, Zhi Zhu, Leiji Zhou, Tianhai Ji, Chaoyong Yang
Enzyme-linked immunosorbent assay (ELISA) is a popular laboratory technique for detection of disease-specific protein biomarkers with high specificity and sensitivity. However, ELISA requires labor-intensive and time-consuming procedures with skilled operators and spectroscopic instrumentation. Simplification of the procedures and miniaturization of the devices are crucial for ELISA-based point-of-care (POC) testing in resource-limited settings. Here, we present a fully integrated, instrument-free, low-cost and portable POC platform which integrates the process of ELISA and the distance readout into a single microfluidic chip...
May 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28524734/capillary-microsampling-in-nonclinical-safety-assessment-practical-sampling-and-bioanalysis-from-a-cro-perspective
#3
David Coleman, Graeme Smith, Rachel Lawrence, Deborah McManus, Sunetha Diaram, Joanna Edwards
Microsampling has the 3R benefits of refining blood collection techniques while reducing the number of animals required for rodent safety assessment studies. There are significant scientific benefits of correlating study findings with systemic exposure and consequently, there is an industry drive to utilize microsampling in regulatory toxicology studies. This article will focus on capillary microsampling and will discuss the initial considerations before using capillary microsampling, study design and some practicalities of sample collection...
May 19, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28521276/an-atmospheric-pressure-ionization-source-using-a-high-voltage-target-compared-to-electrospray-ionization-for-the-lc-ms-analysis-of-pharmaceutical-compounds
#4
Arnaud Lubin, Ronald De Vries, Deirdre Cabooter, Patrick Augustijns, Filip Cuyckens
The type and design of an ionization source can have a significant influence on the performances of a bioanalytical method. It is, therefore, of high interest to evaluate the performances of newly introduced sources to highlight their benefits and limitations in comparison to other well established sources. In this paper, liquid chromatography - mass spectrometry (LC/MS) performances of a new atmospheric pressure ionization (API) source, commercialized as UniSpray, is evaluated. The dynamic range of 24 pharmaceutical and biological compounds is compared between the new API source and electrospray ionization (ESI) for 3 different mobile phase conditions...
May 4, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28520462/activity-based-proteomics-in-bioanalysis-past-present-and-future
#5
Rituraj Dubey, Ravi Bhushan
No abstract text is available yet for this article.
May 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28520459/feedback-from-the-ebf-focus-workshop-bringing-assay-validation-and-analysis-of-biomarkers-into-practice
#6
Philip Timmerman, Marianne Scheel Fjording, John Allinson, Cecilia Arfvidsson, Begoña Barroso, Ulf Diczfalusy, Adrian Freeman, Elizabeth Hickford, Hamza Kandousi, Sidath Katugampola, Ulrich Kunz, Robert Nelson, John Smeraglia
European Bioanalysis Forum, Lisbon, Portugal, 9-10 June 2016 At the recent European Bioanalysis Forum's Focus Workshop 'Bringing Assay Validation and Analysis of Biomarkers into Practice', the discussion on best practice for biomarker assay validation continued. Both the presentations and the adjacent panel discussions yielded valuable food for thought for the broader bioanalytical community. The present conference report summarizes the essence from these discussions and from the proposals or conclusions made by all delegates on how to increase the necessary connectivity of the stakeholders involved in the bioanalysis of biomarkers...
May 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28520455/quantitative-determination-of-avi-7100-radavirsen-a-phosphorodiamidate-morpholino-oligomer-pmoplus-%C3%A2-in-human-plasma-using-lc-ms-ms
#7
Min Meng, Jianbo Zhang, Aihua Liu, Scott Reuschel, Pete Sazani, Michael Wong
AIM: AVI-7100 (Radavirsen) is a 20-mer phosphorodiamidate morpholino oligomer (PMOplus®) for the treatment of influenza. Results/methodology: An automated solid-phase extraction method was used to extract plasma samples (200 μl). The extracts were analyzed using liquid chromatography coupled with tandem mass spectrometry under the positive ionization mode. This method was fully validated over the calibration curve range of 5.00-1000 ng/ml. The between-run precision and accuracy ranged from 0...
May 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28511118/simultaneous-determination-of-selective-serotonin-reuptake-inhibitors-and-their-main-metabolites-in-human-breast-milk-by-liquid-chromatography-electrospray-mass-spectrometry
#8
Etienne Weisskopf, Alice Panchaud, Kim An Nguyen, Delphine Grosjean, Jean-Michel Hascoët, Chantal Csajka, Chin B Eap, Nicolas Ansermot
A bioanalytical method by high performance liquid chromatography coupled to electrospray mass spectrometry (HPLC-ESI-MS), adapted from a previously published method in plasma, was validated in breast milk for the simultaneous quantification of all antidepressants belonging to the class of selective serotonin reuptake inhibitors (citalopram, fluoxetine, fluvoxamine, paroxetine and sertraline) and their major metabolites (desmethylcitalopram and norfluoxetine). Milk samples (250μl) first underwent protein precipitation followed by solid-phase extraction on a reversed phase/cation exchange sorbent...
July 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28508542/dynamic-self-assembly-of-homogenous-microcyclic-structures-controlled-by-a-silver-coated-nanopore
#9
Rui Gao, Yao Lin, Yi-Lun Ying, Xiao-Yuan Liu, Xin Shi, Yong-Xu Hu, Yi-Tao Long, He Tian
The self-assembly of nanoparticles is a challenging process for organizing precise structures with complicated and ingenious structures. In the past decades, a simple, high-efficiency, and reproducible self-assembly method from nanoscale to microscale has been pursued because of the promising and extensive application prospects in bioanalysis, catalysis, photonics, and energy storage. However, microscale self-assembly still faces big challenges including improving the stability and homogeneity as well as pursuing new assembly methods and templates for the uniform self-assembly...
May 16, 2017: Small
https://www.readbyqxmd.com/read/28496058/derivatization-based-high-throughput-bioanalysis-by-lc-ms
#10
Toshimasa Toyo'oka
Liquid chromatography coupled with mass spectrometry (LC-MS) is one of the most prominent analytical techniques due to its inherent selectivity and sensitivity. LC-MS is currently the first choice for high-throughput bioanalysis due to the advancements in MS instruments and the analytical software. Based on this situation, we are developing various types of derivatization reagents, including chiral reagents for MS and/or MS/MS detection. These developed reagents are adopted for the detection of biomarker candidates related to diseases...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28491487/biomolecular-engineering-for-nanobio-bionanotechnology
#11
REVIEW
Teruyuki Nagamune
Biomolecular engineering can be used to purposefully manipulate biomolecules, such as peptides, proteins, nucleic acids and lipids, within the framework of the relations among their structures, functions and properties, as well as their applicability to such areas as developing novel biomaterials, biosensing, bioimaging, and clinical diagnostics and therapeutics. Nanotechnology can also be used to design and tune the sizes, shapes, properties and functionality of nanomaterials. As such, there are considerable overlaps between nanotechnology and biomolecular engineering, in that both are concerned with the structure and behavior of materials on the nanometer scale or smaller...
2017: Nano Convergence
https://www.readbyqxmd.com/read/28489408/thermodynamics-and-mechanisms-of-the-interactions-between-ultra-small-fluorescent-gold-nanoclusters-and-human-serum-albumin-%C3%AE-globulins-and-transferrin-a-spectroscopic-approach
#12
Miao-Miao Yin, Ping Dong, Wen-Qi Chen, Shi-Ping Xu, Liyun Yang, Feng-Lei Jiang, Yi Liu
Noble metal nanoclusters (NCs) show great promise as nanoprobes for bioanalysis and cellular imaging in biological applications due to ultra-small size, good photophysical properties and excellent biocompatibility. In order to achieve a comprehensive understanding of possible biological implications, a series of spectroscopic measurements were conducted under different temperatures to investigate the interactions of Au NCs (~1.7 nm) with three model plasmatic proteins (human serum albumin (HSA), γ-globulins and transferrin)...
May 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28488883/ultrasensitive-bioanalysis-current-status-and-future-trends
#13
Stéphanie Simon, Eric Ezan
Ligand-binding techniques such as immunoassays, the reference for clinical diagnosis, offer a wide range of innovative approaches based on signal DNA amplification, nanotechnologies or digital assays, which result in technologies with sensitivities more than 1000-times that of formats used 20 years ago. Providing that these technologies gain acceptance and translate into robust commercial platforms, we expect that several fields will be impacted in the near future, including the clinical diagnosis of cancer markers, the early detection of infectious diseases and the safety of biotherapeutics...
May 10, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28472287/optimized-and-validated-hplc-analysis-of-st-john-s-wort-extract-and-final-products-by-simultaneous-determination-of-major-ingredients
#14
Konstantina Zeliou, Nikos I Kontaxis, Evangelia Margianni, Christos Petrou, Fotini N Lamari
Aim of this work was to develop a validated high performance liquid chromatography method for the analysis of extracts and final products of St. John's wort, according to international guidelines for bioanalytical method validation. Chromatographic separation was performed on a C18 column with a combination of gradient and isocratic steps; the mobile phase composed of ammonium acetate solution (pH 4.5; 10 mM), acetonitrile and methanol. Quantification and method validation was performed using extract spiked with external reference standards of chlorogenic acid, rutin, hyperoside, isoquercitrin, quercetin and hypericin...
May 3, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28471159/self-assembled-dna-tetrahedral-scaffolds-for-the-construction-of-electrochemiluminescence-biosensor-with-programmable-dna-cyclic-amplification
#15
Qiu-Mei Feng, Yue-Hua Guo, Jing-Juan Xu, Hong-Yuan Chen
A novel DNA tetrahedron-structured electrochemiluminescence (ECL) platform for bioanalysis with programmable DNA cyclic amplification was developed. In this work, glucose oxidase (GOD) was labeled to a DNA sequence (S) as functional conjugation (GOD-S), which could hybridize with other DNA sequences (L and P) to form GOD-S:L:P probe. In the presence of target DNA and a help DNA (A), the programmable DNA cyclic amplification was activated and released GOD-S via toehold-mediated strand displacement. Then, the obtained GOD-S was further immobilized on the DNA tetrahedral scaffolds with a pendant capture DNA and Ru(bpy)3(2+)-conjugated silica nanoparticles (RuSi NPs) decorated on the electrode surface...
May 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28461740/pharmacokinetic-interactions-between-glimepiride-and-rosuvastatin-in-healthy-korean-subjects-does-the-slco1b1-or-cyp2c9-genetic-polymorphism-affect-these-drug-interactions-observations-and-introspection-of-the-bioanalysis
#16
https://www.readbyqxmd.com/read/28458688/long-term-safety-of-pegylated-coagulation-factor-viii-in-the-immune-deficient-rowett-nude-rat
#17
Caroline E Rasmussen, Jette Nowak, Julie M Larsen, Emma Moore, David Bell, Kai Chiu Liu, Nanna Skall Sorensen, Wendela A Kappers, Thomas Krogh-Meibom, Hanne Offenberg
Turoctocog alfa pegol (N8-GP) is a glycoPEGylated human recombinant factor VIII for the treatment of hemophilia A. The safety profile of rFVIII, and polyethylene glycols (PEG) technology, is well-established. Conducting long-term toxicity studies in animals using human proteins can be complicated by anti-drug antibody (ADA) development. To evaluate long-term safety of N8-GP, 26- and 52-week toxicity studies were conducted in immune-deficient rats dosed intravenously every fourth day with 0, 50, 150, 500, or 1200 IU/kg N8-GP...
2017: Journal of Toxicology
https://www.readbyqxmd.com/read/28454735/biosynthesis-inspired-deracemizative-production-of-d-luciferin-by-combining-luciferase-and-thioesterase
#18
Juri Maeda, Dai-Ichiro Kato, Masatoshi Okuda, Masahiro Takeo, Seiji Negoro, Kazunari Arima, Yuji Ito, Kazuki Niwa
Due to the strict enantioselectivity of firefly luciferase, only d-luciferin can be used as a substrate for bioluminescence reactions. Unfortunately, luciferin racemizes easily and accumulation of nonluminous l-luciferin has negative influences on the light emitting reaction. Thus, maintaining the enantiopurity of luciferin in the reaction mixture is one of the most important demands in bioluminescence applications using firefly luciferase. In fireflies, however, l-luciferin is the biosynthetic precursor of d-luciferin, which is produced from the L-form undergoing deracemization...
April 25, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28452007/lps-oleuropein-and-blueberry-extracts-affect-the-survival-morphology-and-phosphoinositide-signalling-in-stimulated-human-endothelial-cells
#19
Vincenza Rita Lo Vasco, Martina Leopizzi, Valeria Di Maio, Tania Di Raimo, Stefania Cesa, Alessandra Masci, Carlo Della Rocca
Endothelial cells (EC) act as leading actors in angiogenesis. Understanding the complex network of signal transduction pathways which regulate angiogenesis might offer insights in the regulation of normal and pathological events, including tumours, vascular, inflammatory and immune diseases. The effects of olive oil and of Blueberry extracts upon the phosphoinositide (PI)-specific phospholipase C (PLC) enzymes were evaluated both in quiescent and inflammatory stimulated human umbilical vein EC (HUVEC) using molecular biology (multiliquid bioanalysis) and immunofluorescence techniques...
April 27, 2017: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/28445850/simultaneous-quantification-of-busulfan-clofarabine-and-f-ara-a-using-isotope-labelled-standards-and-standard-addition-in-plasma-by-lc-ms-ms-for-exposure-monitoring-in-hematopoietic-cell-transplantation-conditioning
#20
Arjen M Punt, Jurgen B Langenhorst, Annelies C Egas, Jaap Jan Boelens, Charlotte van Kesteren, Erik M van Maarseveen
In allogeneic hematopoietic cell transplantation (HCT) it has been shown that over- or underexposure to conditioning agents have an impact on patient outcomes. Conditioning regimens combining busulfan (Bu) and fludarabine (Flu) with or without clofarabine (Clo) are gaining interest worldwide in HCT. To evaluate and possibly adjust full conditioning exposure a simultaneous analysis of Bu, F-ARA-A (active metabolite of Flu) and Clo in one analytical run would be of great interest. However, this is a chromatographical challenge due to the large structural differences of Bu compared to F-ARA-A and Clo...
April 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
keyword
keyword
62825
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"