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Lucie Nováková, Jakub Pavlík, Lucia Chrenková, Ondřej Martinec, Lukáš Červený
This review is a Part II of the series aiming to provide comprehensive overview of currently used antiviral drugs and to show modern approaches to their analysis. While in the Part I antivirals against herpes viruses and antivirals against respiratory viruses were addressed, this part concerns antivirals against hepatitis viruses (B and C) and human immunodeficiency virus (HIV). Many novel antivirals against hepatitis C virus (HCV) and HIV have been introduced into the clinical practice over the last decade...
July 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
Xiangyang Miao, Huizhen Yu, Zhun Gu, Lili Yang, Jiahuan Teng, Ya Cao, Jing Zhao
Peptide self-assembly holds tremendous promise for a range of applications in chemistry and biology. In the work reported here, we explored the potential functions of peptide self-assembly in electrochemical bioanalysis by developing a peptide self-assembly assisted signal labeling strategy for assaying protease activity. The fundamental principle of this assay is that target-protease-catalyzed specific proteolytic cleavage blocks self-assembly between the probe peptide and signal peptide, thus preventing the signal labeling of electroactive silver nanoparticles on the electrode surface, which in turn causes the electrochemical signal to decrease...
October 12, 2017: Analytical and Bioanalytical Chemistry
Tingting Zhang, Yue Gu, Cong Li, Xiaoyi Yan, Nannan Lu, He Liu, Zhiquan Zhang, Hong Zhang
In this paper, a new class of metal-free nanocarbon catalyst - nitrogen (N) and sulfur (S) co-doped graphene quantum dot/graphene (NS-GQD/G) hybrid nanosheets were designed and synthesized for sensitive detection of hydrogen peroxide (H2O2). NS-GQD/G was prepared through two steps. Firstly, graphene quantum dots (GQDs) were self-assembled on graphene nanoplatelets via hydrothermal treatment to constitute hybrid nanosheets, followed by a thermal annealing procedure using the hybrid nanosheets and thiourea to form the NS-GQD/G hybrid nanosheets...
October 12, 2017: ACS Applied Materials & Interfaces
Feifei Lan, Linlin Liang, Yan Zhang, Li Li, Na Ren, Mei Yan, Shenguang Ge, Jinghua Yu
In this work, a chemiluminescence-driven collapsible greeting card-like photoelectrochemical lab-on-paper device (GPECD) with hollow channel was demonstrated, in which target-triggering cascade DNA amplification strategy was ingeniously introduced. The GPECD had the functions of reagents storage and signal collection, and the change of configuration could control fluidic path, reaction time and alterations in electrical connectivity. In addition, three-dimentional reduced graphene oxide affixed Au flower was in situ grown on paper cellulose fiber for achieving excellent conductivity and biocompatibility...
October 17, 2017: ACS Applied Materials & Interfaces
Zhihao Li, Xiangdong Yang, Yanbing Yang, Yaning Tan, Yue He, Meng Liu, Xinwen Liu, Quan Yuan
Peroxidase-mimicking nanozyme offers unique advantages of high stability and low cost over natural peroxidase for applications in bioanalysis, biomedicine and pollution treatment. However, the design of high-efficiency peroxidase-mimicking nanozymes remains a grand challenge. In this work, we adopted a structural design approach through hybridization of cube-CeO2 and Pt nanoparticles to create a novel peroxidase-mimicking nanozyme with high efficiency and excellent stability. Compared to pure cube-CeO2 and Pt nanoparticles, the as-hybridized Pt/cube-CeO2 nanocomposites display much improved activity because of the strong metal-support interaction...
October 9, 2017: Chemistry: a European Journal
Aysu Yarman, Sevinc Kurbanoglu, Katharina J Jetzschmann, Sibel A Ozkan, Ulla Wollenberger, Frieder Scheller
In order to replace bio-macromolecules by stable synthetic materials in separation techniques and bioanalysis biomimetic receptors and catalysts have been developed: Functional monomers are polymerized together with the target analyte and after template removal cavities are formed in the "molecularly imprinted polymer" (MIP) which resemble the active sites of antibodies and enzymes. Staring almost 80 years ago, around 1,100 papers on MIPs were published in 2016. Electropolymerization allows to deposit MIPs directly on voltammetric electrodes or chips for quartz crystal microbalance (QCM) and surface plasmon resonance (SPR)...
October 5, 2017: Current Medicinal Chemistry
Yan J Zhang, Hyun Joo An
No abstract text is available yet for this article.
September 2017: Bioanalysis
Shaolian Zhou, Faye Vazvaei, Luca Ferrari, Jun Qu
No abstract text is available yet for this article.
September 2017: Bioanalysis
Seyyed Mehdi Khoshfetrat, Hasan Bagheri, Masoud A Mehrgardi
A sensitive electrochemiluminescence (ECL) aptasensor for aflatoxin M1 (AFM1) detection by a closed bipolar electrode (BPE) array has been introduced. The thiolated AFM1 aptamer was immobilized on gold nanoparticle-coated magnetic Fe3O4 nanoparticles (Apt-GMNPs). Luminol-functionalized silver nanoparticle-decorated graphene oxide (GO-L-AgNPs) participates in π-π interactions with the unpaired bases of the immobilized aptamer (Apt-GMNPs-GO-L-AgNPs). After the Apt-GMNPs-GO-L-AgNPs were introduced to a gold anodic BPE array, the individual electrodes were subjected to different concentrations of AFM1...
September 21, 2017: Biosensors & Bioelectronics
Chunxiang Li, Weisen Lu, Ming Zhu, Bo Tang
The performance of the photoelectrochemical (PEC) bioanalysis relies closely on the properties of the used photoactive species. In this study, a visible-light induced PEC active species, [(C6)2Ir(dcbpy)](+)PF6(-) (C6 = coumarin 6, dcbpy = 2,2'-bipyridine-4,4'-dicarboxylic acid) was prepared based on C6 with the stronger absorbance in the visible region. The as-prepared complex was characterized by (1)H NMR, UV-visible absorption and cyclic voltammetry. It exhibits intense absorption in visible region at 480 nm with a molar extinction coefficient (ε) of more 40000 M(-1) cm(-1), which is approximately twice that of Ru(bpy)3(2+)...
October 17, 2017: Analytical Chemistry
Herbert F Birnboeck, Eginhard Schick, Nicole Justies
No abstract text is available yet for this article.
September 18, 2017: Bioanalysis
Chaoyan Lou, Yan Zhu
No abstract text is available yet for this article.
September 13, 2017: Bioanalysis
Omnia A Ismaiel, William R Mylott, Rand G Jenkins
No abstract text is available yet for this article.
September 13, 2017: Bioanalysis
Imam Pasha S, Murali Balaram Varanasi, Ibrahim Mohammed
Dimethyl fumarate (DMF) is the methyl ester of fumaric acid, after oral administration completely converts to its active metabolite monomethyl fumarate (MMF). A simple, rapid and sensitive LC-MS/MS method was developed and validated for the quantification of MMF in human plasma. Monomethyl fumarate d3 was used as an internal standard (IS). The analyte and the IS were extracted from plasma using a selective solid phase extraction technique. The clean samples were chromatographed on a C18 column using formic acid and acetonitrile (25:75, v/v) as mobile phase...
August 23, 2017: Journal of Pharmaceutical and Biomedical Analysis
Yu Gu, Juan Song, Mei-Xing Li, Ting-Ting Zhang, Wei Zhao, Jing-Juan Xu, Maili Liu, Hong-Yuan Chen
Quantification of trace serum circulate microRNAs is extremely important in clinical diagnosis but remains a great challenge. Herein we developed an ultrasensitive platform for microRNA 141 (miR-141) detection based on a silver coated gold nanorods (Au@Ag NRs) etching process accompanied by surface plasmon resonance (SPR) shift. Both SPR absorption and scattering responses were monitored. Combined amplification cascades of catalyzed hairpin assembly (CHA) and hybridization chain reaction (HCR) with the sensitive SPR responses of plasmonic Au@Ag NRs, the proposed bioassay exhibited ultrahigh sensitivity toward miRNA-141 with dynamic range from 5...
September 19, 2017: Analytical Chemistry
Nagayoshi Asano, Justin Michael Wiseman, Fumio Tsuji, Kouichi Kawazu
Meibomian gland dysfunction (MGD) is the leading cause of dry eye, and although it affects approximately 4% of the population, treatment options remain limited. Topical azithromycin is one of the most promising pharmacological agents because of its multiple mechanisms of action and long sustainability. Azithromycin is frequently used as an off-label medication in the U.S. However, although azithromycin is presumed to act directly on meibomian gland cells, the mechanisms of action that contribute to its clinical efficacy remain unclear because no studies using a pharmacokinetic approach have been performed...
2017: Biological & Pharmaceutical Bulletin
Ling Xu, Laura E Packer, Chao Li, Kojo Abdul-Hadi, Petter Veiby
The current industry practice for antibody-drug conjugate (ADC) bioanalysis includes quantification of total antibody and antibody-conjugated drug. Here, we report a novel 2-in-1 approach for measuring total antibody and protease-cleavable conjugated drug Monomethyl Auristatin E (MMAE) concurrently. This allows for the determination of the DAR (Drug Antibody Ratio) for in vivo samples, with a 3-orders linear range based on total antibody concentration from 0.1 to 100 μg/mL. Our generic, concurrent method has been cross-validated with the previously established methods in an animal study...
September 1, 2017: Analytical Biochemistry
Xiwei Huang, Umar Farooq, Jin Chen, Yakun Ge, Haijun Gao, Jiangtao Su, Xiang Wang, Shurong Dong, Ji-Kui Luo
The future point-of-care diagnostics requires miniaturizing the existing bulky and expensive bioanalysis instruments, where lab-on-CMOS-chip-based technology can provide a promising solution. In this paper, we presented a surface acoustic wave (SAW) pumped lensless microfluidic imaging system for flowing cell detection and counting. Different from the previous lensless systems, which employ external bulky syringe pump for cell driven, the developed system directly integrates the SAW pump on the CMOS image sensor chip to drive the cell-containing microfluid...
August 29, 2017: IEEE Transactions on Biomedical Circuits and Systems
Jumah Masoud Mohammad Salmani, Xue Wu, Joe Antony Jacob, Rong Fu, Baoan Chen
In the current paper, an HPLC/UV method was developed and validated for determination of wogonin in plasma. Considerable attention was paid to the preparation of standard samples and factors affecting drug distribution. A preparation procedure was devised to simulate the conditions the drug is expected to experience in vivo while pointing to the shortcomings of previously published methods. The method was validated according to the FDA regulations and showed to be highly efficient and capable of extracting the drug and IS from the plasma accurately and precisely within the specified range of 50-500 ng mL-1...
September 1, 2017: Acta Pharmaceutica
Frederic D C Martin, Amanda Benjamin, Ruth MacLean, David M Hollinshead, Claire Landqvist
In 2012, AstraZeneca entered into a strategic relationship with Charles River Laboratories whereby preclinical safety packages comprising safety pharmacology, toxicology, formulation analysis, in vivo ADME, bioanalysis and pharmacokinetics studies are outsourced. New processes were put in place to ensure seamless workflows with the aim of accelerating the delivery of new medicines to patients. Here, we describe in more detail the AstraZeneca preclinical safety outsourcing model and the way in which a collaborative tool has helped to translate the processes in AstraZeneca and Charles River Laboratories into simpler integrated workflows that are efficient and visible across the two companies...
August 26, 2017: Drug Discovery Today
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