Cláudio Pinheiro, Niké Guilbert, Lien Lippens, Quentin Roux, Robin Boiy, Suzanne Fischer, Sofie Van Dorpe, Bram De Craene, Geert Berx, Tom Boterberg, Gwen Sys, Hannelore Denys, Ilkka Miinalainen, Pieter Mestdagh, Jo Vandesompele, Olivier De Wever, An Hendrix
Extracellular vesicles (EVs) contain a plethora of biomolecules, including nucleic acids, with diverse diagnostic and therapeutic application potential. Although reverse transcription-quantitative PCR (RT-qPCR) is the most widely applied laboratory technique to evaluate gene expression, its applicability in EV research is challenged by the lack of universal and stably present reference genes (RGs). In this study, we identify, validate and establish SNRPG, OST4, TOMM7 and NOP10 as RGs for the normalization of EV-associated genes by RT-qPCR...
April 2024: Journal of Extracellular Vesicles