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Tsubasa Okano, Yuki Tsujita, Hirokazu Kanegane, Kanako Mitsui-Sekinaka, Kay Tanita, Satoshi Miyamoto, Tzu-Wen Yeh, Motoi Yamashita, Naomi Terada, Yumi Ogura, Masatoshi Takagi, Kohsuke Imai, Shigeaki Nonoyama, Tomohiro Morio
OBJECTIVE: In the current study, we aimed to accurately evaluate donor/recipient or male/female chimerism in samples from patients who underwent hematopoietic stem cell transplantation (HSCT). METHODS: We designed the droplet digital polymerase chain reaction (ddPCR) for SRY and RPP30 to detect the male/female chimerism. We also developed mutation-specific ddPCR for four primary immunodeficiency diseases. RESULTS: The accuracy of the male/female chimerism analysis using ddPCR was confirmed by comparing the results with those of conventional methods (fluorescence in situ hybridization and short tandem repeat-PCR) and evaluating dilution assays...
April 18, 2018: Journal of Clinical Immunology
Qing Xiao, Taishan Min, Shuangping Ma, Lingna Hu, Hongyan Chen, Daru Lu
Targeted integration of transgenes facilitates functional genomic research and holds prospect for gene therapy. The established microhomology-mediated end-joining (MMEJ)-based strategy leads to the precise gene knock-in with easily constructed donor, yet the limited efficiency remains to be further improved. Here, we show that single-strand DNA (ssDNA) donor contributes to efficient increase of knock-in efficiency and establishes a method to achieve the intracellular linearization of long ssDNA donor. We identified that the CRISPR/Cas9 system is responsible for breaking double-strand DNA (dsDNA) of palindromic structure in inverted terminal repeats (ITRs) region of recombinant adeno-associated virus (AAV), leading to the inhibition of viral second-strand DNA synthesis...
April 18, 2018: Molecular Genetics and Genomics: MGG
Bo-Yeon Lee, Qiang Li, Woo-Jin Song, Hyung-Kyu Chae, Kyeong Kweon, Jin-Ok Ahn, Hwa-Young Youn
Cytotherapy with mesenchymal stem cells (MSCs) has been studied in many species, and often requires in vitro cell expansion to obtain therapeutic doses of stem cells. Because the characteristics of MSCs, such as self-renewal and multi-lineage differentiation, can be altered by long-term culture, it is important to maintain stemness during cultivation. This study assessed the changes in the characteristics of feline adipose tissue-derived (fAT)-MSCs during in vitro passaging. Stem cells isolated from the adipose tissue of donor cats were cultured for seven sub-passages...
April 18, 2018: Journal of Veterinary Medical Science
Savneet Kaur Bains, Kim Chapman, Scott Bright, Anish Senan, Munira Kadhim, Predrag Slijepcevic
PURPOSE: To investigate the effects of ionising radiation on telomere length and telomerase activity in Human Lens Epithelial cells. There are studies suggesting evidence of telomere length in association with opacity of the lens, however, these studies have been conducted on Canine Lens cells. Our study was designed to understand further the effects of different doses of ionising radiation on telomere length and telomerase activity in cultured Human Lens Epithelium cells from three Donors...
April 18, 2018: International Journal of Radiation Biology
Yan Zhou, Li-Lan Li, Zhou-Lin Zhong, Xue-Jun Liu, Jin-Lian Liu, Wei-Dong Shen, Guo-Guang Wu
OBJECTIVE: To analyse the cases of platelet transfusion refractoriness after received HLA-matched unrelated donor hematopoietic stem cell transplantation, to analyze and identify the phenotype and genotype of CD36 in both the patient and stem cell donor, as well as the characteristic of antibody induced platelet transfusion refractoriness, and to analyse the efficacy of matched CD36-deficiency platelets transfusions. METHODS: The CD36 expression on platelet and monocyte was analyzed by flow cytometry (FCM) in both patient and donor...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Leila Pirayeshfard, Zohreh Sharifi, Sedigheh Amini-Kafiabad, Nasrin Haghnazari Sadaghiani
BACKGROUND: Human T-cell leukemia virus (HTLV) has been associated with various disease types. Since the discovery of the virus in 1980, seven subtypes of the virus have been identified. HTLV is widespread and endemic in some regions, such as Japan, Africa, South America, and northeast Iran. This study aimed to identify HTLV-1 genotype and also to analyze the nucleotide sequence of the LTR region in three groups, including blood donors, HIV-1+ patients, and β-thalassemia patients. MATERIALS AND METHODS: In this cross-sectional study, 2200 samples were collected from blood donors in Tehran (2000 samples), HIV-1+ patients (100 samples) and β-thalassemia patients (100 samples)...
April 16, 2018: Journal of Medical Virology
Cuicui Lyu, Jun Shen, Rui Wang, Haihui Gu, Jianping Zhang, Feng Xue, Xiaofan Liu, Wei Liu, Rongfeng Fu, Liyan Zhang, Huiyuan Li, Xiaobing Zhang, Tao Cheng, Renchi Yang, Lei Zhang
BACKGROUND: Replacement therapy for hemophilia remains a lifelong treatment. Only gene therapy can cure hemophilia at a fundamental level. The clustered regularly interspaced short palindromic repeats-CRISPR associated nuclease 9 (CRISPR-Cas9) system is a versatile and convenient genome editing tool which can be applied to gene therapy for hemophilia. METHODS: A patient's induced pluripotent stem cells (iPSCs) were generated from their peripheral blood mononuclear cells (PBMNCs) using episomal vectors...
April 6, 2018: Stem Cell Research & Therapy
Xi Tang, Jean-Pierre Allain, Hao Wang, Xia Rong, Jinyan Chen, Ke Huang, Ru Xu, Min Wang, Jieting Huang, Qiao Liao, Zhengang Shan, Shengxue Luo, Tingting Li, Chengyao Li, Yongshui Fu
This study was carried out to determine the incidence of HBV infection in the young generation born after mandatory implementation of hepatitis B vaccination since 1992. Repeat blood donors born between 1992 and 1997 were enrolled, who gave blood at least twice during the past three years. Donors were tested for HBV infection markers of HBsAg, anti-HBc, anti-HBs and viral DNA by immunoassays (EIAs) and nucleic acid tests (NAT). A total of 14937 pre-donation screening qualified young repeat donors aged 18-23 were tested with 9 (0...
April 6, 2018: Journal of Viral Hepatitis
Jameson Clover, Amy Ansin, Christopher G Stoeger, Mark A Terry, Khoa D Tran
PURPOSE: To compare tissue evaluation times and specular image quality before and after the implementation of rapid tissue warming at a single eye bank. METHODS: This retrospective study compares the evaluation of 494 donor corneas. All corneas were stored in Optisol-GS. Group 1 contained 247 corneas evaluated immediately before implementation of a tissue incubator and tissue warming protocol. Group 2 contained 247 corneas evaluated immediately after implementation...
April 4, 2018: Cornea
Agne Laucyte-Cibulskiene, Evelina Boreikaite, Gediminas Aucina, Migle Gudynaite, Ilona Rudminiene, Sigita Anisko, Loreta Vareikiene, Liutauras Gumbys, Dileta Valanciene, Ligita Ryliskyte, Kestutis Strupas, Laurynas Rimsevicius, Marius Miglinas
Vascular calcification (VC) is linked to post-transplant cardiovascular events and hypercalcemia which may influence kidney graft function in the long term. We aimed to evaluate whether pretransplant aortic arch calcification (AoAC) can predict post-transplant cardiovascular or cerebrovascular events (CVEs), and to assess its association with post-transplant plasma calcium levels and renal function in one-year follow-up. Our single-center observational prospective study enrolled 37 kidney transplant recipients (KTR) without previous history of vascular events...
November 2018: Renal Failure
Evan M Bloch, Christi E Marshall, Joan S Boyd, Lisa Shifflett, Aaron A R Tobian, Eric A Gehrie, Paul M Ness
BACKGROUND: Bacterial contamination of platelets remains a major transfusion-associated risk despite long-standing safety measures in the United States. We evaluated an approach using secondary bacterial culture (SBC) to contend with residual risk of bacterial contamination. STUDY DESIGN AND METHODS: Phased implementation of SBC was initiated in October 2016 for platelets (all apheresis collected) received at our institution from the blood donor center (Day 3 post collection)...
April 1, 2018: Transfusion
Yun Qiu, Yichen Wu, Min Meng, Man Luo, Hongmei Zhao, Hong Sun, Sumin Gao
BACKGROUND: This study explores the protective effects of a hydrogen sulfide donor, morpholin-4-ium 4-methoxyphenyl-morpholino-phosphinodithioate (GYY4137), in the hearts of diabetic mice that had been subjected to myocardial ischemia/reperfusion injury. Diabetes impairs the Akt pathway, in which the Akt protein is dephosphorylated and inactivated by PH domain leucine-rich repeat protein phosphatase-1 (PHLPP-1). However, the function of PHLPP-1 and molecular mechanism that underlies the cardiac protection exerted by GYY4137 remains unknown...
May 2018: Journal of Surgical Research
Ho Joong Choi, Dong Goo Kim, Yumi Kim, Bong Jun Kwak, Jae Hyun Han, Tae Ho Hong, Young Kyoung You
Background Hepatic artery thrombosis (HAT) can result in biliary tree necrosis and graft loss necessitating re-transplantation. The most effective treatment approach is still controversial. This study was performed to review the outcomes of HAT after living donor liver transplantation (LDLT), and to clarify the feasibility of different strategies. Methods From May 1996 to August 2017, LDLT using the right lobe was performed in 827 adult patients in our center. Our technique of hepatic artery (HA) reconstruction is end-to-end anastomosis under a microscope (× 10)...
March 31, 2018: Liver Transplantation
Yanru Cui, Wenying Zhang, Xiuyun Lin, Shizhong Xu, Jianlong Xu, Zhikang Li
Drought is the most important factor limiting rice yield in most rainfed areas of Asia and Africa. Four large BC2 F2 populations consisted of 3,200 individuals, which were derived from crosses between an elite Geng variety, Jigeng88, and four donors from three different countries, were screened and progeny tested under severe drought stress, resulting in the development of 72 introgression lines (ILs) with significantly improved yield compared to the recurrent parent Jigeng88. These DT ILs plus four random populations (without drought selection population) from the same crosses were evaluated in replicated trials under both drought stress and non-stress conditions in two environments, and characterized with simple sequence repeat (SSR) markers to understand how directional selection was operating on the genetic variation of DT of rice...
2018: Frontiers in Plant Science
Ivana Grabundzija, Alison B Hickman, Fred Dyda
Helitrons are eukaryotic DNA transposons that have profoundly affected genome variability via capture and mobilization of host genomic sequences. Defining their mode of action is therefore important for understanding how genome landscapes evolve. Sequence similarities with certain prokaryotic mobile elements suggest a "rolling circle" mode of transposition, involving only a single transposon strand. Using the reconstituted Helraiser transposon to study Helitron transposition in cells and in vitro, we show that the donor site must be double-stranded and that single-stranded donors will not suffice...
March 29, 2018: Nature Communications
Bernadett Palyi, Nora Magyar, Judit Henczko, Balint Szalai, Agnes Farkas, Thomas Strecker, Maria Takacs, Zoltan Kis
In 2013-2016, West Africa experienced the largest and longest Ebola virus disease outbreak ever documented. The wide geographic spread and magnitude of the outbreak often limited the timely and rapid testing of diagnostic samples from patients with suspected Ebola virus disease, raising questions regarding the optimal storage and shipping conditions of clinically relevant specimens, including EDTA-whole blood, plasma, capillary blood, urine and seminal fluid (associated with sexual transmission of the Ebola virus after recovery from the disease)...
March 29, 2018: Emerging Microbes & Infections
Juhwan Park, Je-Kyun Park
A blood cross-matching test should be carried out to prevent a hemolytic transfusion reaction as the final verification step. To simplify complicated procedures of a conventional blood cross-matching test requiring bulky systems and skilled people, we present a finger-actuated microfluidic device for the blood cross-matching test. Although finger actuation is a simple action that anyone can easily accomplish, there would be a variation in the individual finger actuation that may induce the user-dependent errors of the device...
March 28, 2018: Lab on a Chip
E M Bloch, Y Yang, M He, L Tonnetti, Y Liu, J Wang, Y Guo, H Li, D A Leiby, H Shan
BACKGROUND AND OBJECTIVES: Babesia spp. are tick-borne, intraerythrocytic protozoan parasites, several of which are transfusion-transmissible. Transfusion-transmitted babesiosis poses serious risk to a diverse patient population, including neonates, patients aged >50 years, the asplenic and the immunocompromised that are over-represented among transfusion recipients. Despite reports of B. microti and B. venatorum in People's Republic of China (PRC), no surveillance of blood donors for Babesia has previously been undertaken...
March 26, 2018: Vox Sanguinis
Edith Acquaye-Seedah, Elizabeth E Reczek, Hugh H Russell, Andrea M DiVenere, Sara O Sandman, Joseph H Collins, Caitlin A Stein, Timothy A Whitehead, Jennifer A Maynard
Despite high vaccination rates, the incidence of whooping cough has been steadily increasing in developing countries for several decades. The current acellular pertussis (aP) vaccines all include the major protective antigen pertussis toxin (PTx) and are safer, but appear less protective than infection or older whole cell vaccines. To better understand the attributes of individual antibodies stimulated by aP, we isolated plasmablast clones recognizing PTx after booster immunization of two donors. Five unique antibody sequences recognizing native PTx were recovered and expressed as recombinant human IgG1 antibodies...
March 26, 2018: Infection and Immunity
Ahmed Mohamed Sultan, Ahmed Shehta, Tarek Salah, Mohamed Elshoubary, Mohamed Abdel Wahab
INTRODUCTION: Spontaneous migration of synthetic grafts had been described after abdominal vascular procedures into the duodenum and small bowel. In the setting of liver transplantation, synthetic grafts are commonly used for overcoming shortened or poor-quality vasculatures. We describe a case of spontaneous migration of thrombosed synthetic vascular graft after living-donor liver transplantation (LDLT). PRESENTATION: A 59 years male patient with end stage liver disease underwent LDLT utilizing a right hemi-liver graft...
March 15, 2018: International Journal of Surgery Case Reports
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