keyword
https://read.qxmd.com/read/38547092/oxidative-study-a-pilot-prospective-observational-cohort-study-protocol-examining-the-influence-of-peri-reperfusion-hyperoxemia-and-immune-dysregulation-on-early-allograft-dysfunction-after-orthotopic-liver-transplantation
#1
JOURNAL ARTICLE
Elizabeth A Wilson, Anna Woodbury, Kirsten M Williams, Craig M Coopersmith
Early allograft dysfunction (EAD) is a functional hepatic insufficiency within a week of orthotopic liver transplantation (OLT) and is associated with morbidity and mortality. The etiology of EAD is multifactorial and largely driven by ischemia reperfusion injury (IRI), a phenomenon characterized by oxygen scarcity followed by paradoxical oxidative stress and inflammation. With the expanded use of marginal allografts more susceptible to IRI, the incidence of EAD may be increasing. This necessitates an in-depth understanding of the innate molecular mechanisms underlying EAD and interventions to mitigate its impact...
2024: PloS One
https://read.qxmd.com/read/38544668/intrauterine-autologous-platelet-rich-plasma-treatment-in-women-with-at-least-two-implantation-failures-a-retrospective-cohort-study
#2
JOURNAL ARTICLE
Neda Fattahi Meybodi, Maryam Eftekhar, Behnaz Gandom
BACKGROUND: Finding the most effective way to improve implantation rate in women who are receiving assisted reproductive technology treatment is still a challenge. OBJECTIVE: This study aimed to assess the pregnancy outcomes of intrauterine platelet-rich plasma (PRP) therapy in women with a history of at least 2 implantation failures. MATERIALS AND METHODS: In this retrospective cohort study, data of 852 women who were candidates for frozen-thawed embryo transfer was extracted from their medical records from April 2017 to September 2021 at Yazd Reproductive Sciences Institute, Yazd, Iran...
January 2024: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://read.qxmd.com/read/38543918/anti-sars-cov-2-igg-seroprevalence-in-tyrol-austria-among-28-768-blood-donors-between-may-2022-and-march-2023
#3
JOURNAL ARTICLE
Anita Siller, Lisa Seekircher, Manfred Astl, Lena Tschiderer, Gregor A Wachter, Julia Penz, Bernhard Pfeifer, Andreas Huber, Manfred Gaber, Harald Schennach, Peter Willeit
BACKGROUND: To provide updated estimates on SARS-CoV-2 antibody seroprevalence and average antibody titres for Central Europe. METHODS: In repeat cross-sectional investigations (1 May 2022 to 9 March 2023) involving 28,768 blood donors in the Federal State of Tyrol, Austria (participation rate: 87.0%), we measured Spike receptor-binding domain (RBD) and Nucleocapsid IgG antibodies (37,065 and 12,645 samples), and estimated monthly seroprevalences and geometric mean titres...
March 8, 2024: Vaccines
https://read.qxmd.com/read/38539134/attitudes-of-oncology-patients-towards-biospecimen-donation-for-biobank-research
#4
JOURNAL ARTICLE
Jan Domaradzki, Justyna Czekajewska, Dariusz Walkowiak
BACKGROUND: Since the biological material that remains after diagnostic and therapeutic procedures plays crucial role in biobank research, this study aims to explore cancer patients' views on the donation of biospecimens for research purposes. METHODS: 548 oncology patients from two hospitals with oncology treatment units in Poznan, Poland, completed an anonymous, self-administered pen-and-paper questionnaire. RESULTS: Although only 43.4% of patients had heard of biobanks, 93...
March 27, 2024: BMC Cancer
https://read.qxmd.com/read/38537318/effects-of-donor-age-and-reproductive-history-on-developmental-potential-of-ovum-pickup-oocytes-in-japanese-black-cattle-wagyu
#5
JOURNAL ARTICLE
Yinyan Du, Yuhan Xia, Jiahui Xu, Zhihui Liu, Zhentian Liu, Meijie Zhang, Guangyong Xu, Xuesong Xing, Fuliang Du
The objectives of this study were to analyze the (1) effects of donor age and multiparity on development of in vitro fertilization (IVF) embryos after ovum pickup (OPU), (2) effects of repeated and consecutive OPU-IVF procedures on embryo development, and (3) embryo production from OPU-IVF in donors with differing embryo yields after multiple ovulation and embryo transfer technology (MOET) in Japanese Black cattle (Wagyu). Donors were pre-treated with low-dosage follicle-stimulating hormone (FSH; 200 IU total), and oocytes were collected via OPU and fertilized by IVF to generate blastocysts...
March 22, 2024: Theriogenology
https://read.qxmd.com/read/38521227/fecal-microbiota-transplantation-stimulates-type-2-and-tolerogenic-immune-responses-in-a-mouse-model
#6
JOURNAL ARTICLE
G Brett Moreau, Farha Naz, William A Petri
OBJECTIVES: Clostridioides difficile infection (CDI) is the leading hospital-acquired infection in North America. While previous work on fecal microbiota transplantation (FMT), a highly effective treatment for CDI, has focused on colonization resistance mounted against C. difficile by FMT-delivered commensals, the effects of FMT on host gene expression are relatively unexplored. This study aims to identify transcriptional changes associated with FMT, particularly changes associated with protective immune responses...
March 21, 2024: Anaerobe
https://read.qxmd.com/read/38489061/targeted-gene-insertion-the-cutting-edge-of-crispr-drug-development-with-hemophilia-as-a-highlight
#7
REVIEW
Zhenjie Zhang, Siqi Zhang, Hoi Ting Wong, Dali Li, Bo Feng
The remarkable advance in gene editing technology presents unparalleled opportunities for transforming medicine and finding cures for hereditary diseases. Human trials of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9)-based therapeutics have demonstrated promising results in disrupting or deleting target sequences to treat specific diseases. However, the potential of targeted gene insertion approaches, which offer distinct advantages over disruption/deletion methods, remains largely unexplored in human trials due to intricate technical obstacles and safety concerns...
March 15, 2024: BioDrugs: Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy
https://read.qxmd.com/read/38478469/a-nanopore-based-cucumber-genome-assembly-reveals-structural-variations-at-two-qtls-controlling-hypocotyl-elongation
#8
JOURNAL ARTICLE
Bin Liu, Cheng-Cheng Shen, Shi-Wei Xia, Shan-Shan Song, Li-Hong Su, Yu Li, Qian Hao, Yan-Jun Liu, Dai-Lu Guan, Ning Wang, Wen-Jiao Wang, Xiang Zhao, Huan-Xiu Li, Xi-Xiang Li, Yun-Song Lai
The Xishuangbanna (XIS) cucumber (Cucumis sativus var. xishuangbannanesis) is a semiwild variety that has many distinct agronomic traits. Here, long -reads generated by Nanopore sequencing technology helped assembling a high-quality genome (contig N50 = 8.7 Mb) of landrace XIS49. A total of 10,036 structural/sequence variations (SVs) were identified when comparing with Chinese Long (CL), and known SVs controlling spines, tubercles, and carpel number were confirmed in XIS49 genome. Two QTLs of hypocotyl elongation under low light, SH3...
March 13, 2024: Plant Physiology
https://read.qxmd.com/read/38477177/the-impact-of-active-cytomegalovirus-infection-on-donor-derived-cell-free-dna-testing-in-heart-transplant-recipients
#9
JOURNAL ARTICLE
Amit H Alam, Johanna Van Zyl, Hira I Shakoor, Dana Farsakh, Ahmad B Abdelrehim, Neville Maliakkal, Aayla K Jamil, Raksha Patel, Joost Felius, Staci McKean, Shelley A Hall
BACKGROUND: Little is known about the relationship between cytomegalovirus (CMV) infections and donor-derived cell-free DNA (dd-cfDNA) in heart transplant recipients. METHODS: In our study, CMV and dd-cfDNA results were prospectively collected on single-organ heart transplant recipients. If the CMV study was positive, a CMV study with dd-cfDNA was repeated 1-3 months later. The primary aim was to compare dd-cfDNA between patients with positive and negative CMV results...
March 2024: Clinical Transplantation
https://read.qxmd.com/read/38474058/utility-of-next-generation-sequencing-based-chimerism-analysis-for-early-relapse-prediction-following-allogenic-hematopoietic-cell-transplantation
#10
JOURNAL ARTICLE
Heerah Lee, Seung-Won Chae, Sung Im Cho, Jee-Soo Lee, Man Jin Kim, Moon-Woo Seong
Chimerism monitoring following allogeneic hematopoietic cell transplantation (HCT) plays a pivotal role in evaluating engraftment status and identifying early indicators of relapse. Recent advancements in next-generation sequencing (NGS) technology have introduced AlloSeq HCT as a more sensitive alternative to short tandem repeat (STR) analysis. This study aimed to compare AlloSeq HCT with STR, focusing on the prediction of early relapse post-allogeneic HCT. Chimerism levels in 29 HCT recipients were assessed using both STR and NGS, employing a total of 125 whole blood or bone marrow aspirate samples (68 post-HCT and 57 pre-HCT samples from recipients or donors)...
February 28, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473423/transcriptomic-proteomic-and-genomic-mutational-fraction-differences-based-on-hpv-status-observed-in-patient-derived-xenograft-models-of-penile-squamous-cell-carcinoma
#11
JOURNAL ARTICLE
Niki M Zacharias, Luis Segarra, Keiko Akagi, Natalie Wall Fowlkes, Huiqin Chen, Angelita Alaniz, Carolyn de la Cerda, Pedro Pesquera, Yuanxin Xi, Jing Wang, Jad Chahoud, Xin Lu, Priya Rao, Magaly Martinez-Ferrer, Curtis A Pettaway
Metastatic penile squamous cell carcinoma (PSCC) has only a 50% response rate to first-line combination chemotherapies and there are currently no targeted-therapy approaches. Therefore, we have an urgent need in advanced-PSCC treatment to find novel therapies. Approximately half of all PSCC cases are positive for high-risk human papillomavirus (HR-HPV). Our objective was to generate HPV-positive (HPV+) and HPV-negative (HPV-) patient-derived xenograft (PDX) models and to determine the biological differences between HPV+ and HPV- disease...
March 6, 2024: Cancers
https://read.qxmd.com/read/38457465/network-analysis-of-spread-of-sars-cov-2-within-dialysis-clinics-a-multi-center-network-analysis
#12
JOURNAL ARTICLE
Sunpeng Duan, Yuedong Wang, Peter Kotanko, Hanjie Zhang
BACKGROUND: In-center hemodialysis entails repeated interactions between patients and clinic staff, potentially facilitating the spread of COVID-19. We examined if in-center hemodialysis is associated with the spread of SARS-CoV-2 between patients. METHODS: Our retrospective analysis comprised all patients receiving hemodialysis in four New York City clinics between March 12th, 2020, and August 31st, 2022. Treatment-level clinic ID, dialysis shift, dialysis machine station, and date of COVID-19 diagnosis by RT-PCR were documented...
2024: PloS One
https://read.qxmd.com/read/38455485/using-extracorporeal-membrane-oxygenation-in-donations-after-cardiac-death-or-brain-death-a-single-center-experience-and-long-term-outcome
#13
JOURNAL ARTICLE
Chia-En Hsieh, Ya-Lan Hsu, Yao-Li Chen, Hsin-Rou Liang, Kuo-Hua Lin, Wen-Yuan Chen, Hsiu-Man Wu, Sin-Bao Hunang, Yu-Ju Hung
AIMS: The use of extended criteria donors is a routine practice that sometimes involves extracorporeal membrane oxygenation (ECMO) in donations after cardiac death or brain death. METHODS: We performed a retrospective study in a single center from January 2006 to December 2019. The study included 90 deceased donor liver transplants. The patients were divided into three groups: the donation after brain death (DBD) group ( n  = 58, 64.4%), the DBD with ECMO group ( n  = 11, 12...
March 2024: Annals of Gastroenterological Surgery
https://read.qxmd.com/read/38450982/expansion-and-improvement-of-chinamu-by-mut-seq-and-chromosome-level-assembly-of-the-mu-starter-genome
#14
JOURNAL ARTICLE
Lei Liang, Yuancong Wang, Yanbin Han, Yicong Chen, Mengfei Li, Yibo Wu, Zeyang Ma, Han Zhao, Rentao Song
ChinaMu is the largest sequence-indexed Mutator (Mu) transposon insertional library in maize (Zea mays). In this study, we made significant improvements to the size and quality of the ChinaMu library. We developed a new Mu-tag isolation method Mu-Tn5-seq (MuT-seq). Compared to the previous method used by ChinaMu, MuT-seq recovered 1/3 more germinal insertions, while requiring only about 1/14 of the sequencing volume and 1/5 of the experimental time. Using MuT-seq, we identified 113,879 germinal insertions from 3,168 Mu-active F1 families...
March 7, 2024: Journal of Integrative Plant Biology
https://read.qxmd.com/read/38450465/-outcomes-of-hemi-descemet-membrane-endothelial-keratoplasty-and-phacoemulsification-for-the-treatment-of-primary-fuchs-endothelial-corneal-dystrophy-combined-with-cataract
#15
JOURNAL ARTICLE
B E Malyugin, A M Geliastanov, O P Antonova, E A Andreeva, M V Poletaeva, R S Isabekov
PURPOSE: This study evaluates the long-term results of surgical treatment of patients with Fuchs' endothelial corneal dystrophy and cataract. MATERIAL AND METHODS: The study included 24 patients (24 eyes) with primary Fuchs' endothelial corneal dystrophy and cataract, who underwent cataract phacoemulsification with IOL implantation and of Descemet's membrane endothelial keratoplasty with a semicircular graft (hemi-DMEK). The effect of treatment was assessed by best corrected visual acuity (BCVA), central corneal thickness (CCT) and endothelial cell density (ECD)...
2024: Vestnik Oftalmologii
https://read.qxmd.com/read/38449375/interviews-with-lay-caregivers-about-their-experiences-supporting-patients-throughout-kidney-transplantation
#16
JOURNAL ARTICLE
Anne Solbu, Renee B Cadzow, Teresa Pullano, Sherry Brinser-Day, Laurene Tumiel-Berhalter, Liise K Kayler
Introduction: Lay caregivers provide essential support to patients throughout the kidney transplant process, pretransplant through discharge. Sparse data exists about kidney transplant caregiver experience and facilitators of caregiver engagement. The aim of this study was to explore and describe lay caregivers' accounts of supporting a patient before and early after kidney transplantation. Methods: Caregivers of recent kidney transplant recipients were individually interviewed about their experiences, coping strategies, and perspectives of center-specific support approaches for patients and caregivers in a single transplant center...
March 6, 2024: Progress in Transplantation
https://read.qxmd.com/read/38442682/circumventing-aglycon-transfer-en-route-to-the-synthesis-of-pentasaccharide-thioglycoside-donor-for-the-chain-extension-of-plesiomonas-shigelloides-strain-302-73-serotype-o1-repeating-unit
#17
JOURNAL ARTICLE
Balasaheb K Ghotekar, Sayantan Biswas, Suvarn S Kulkarni
Herein we report a chemical synthesis of a pentasaccharide thioglycoside repeating unit of Plesiomonas shigelloides Strain 302-73 (Serotype O1), as a chain extension unit. In our synthetic endeavor we encountered multiple aglycon transfer reactions during glycosylations. This problem was obviated by employing a PMP group as a transient protecting group.
March 1, 2024: Carbohydrate Research
https://read.qxmd.com/read/38434420/effects-of-temperature-anomaly-on-sperm-quality-a-multi-center-study-of-33-234-men
#18
JOURNAL ARTICLE
Lina Xiao, Qiling Wang, Haobo Ni, Ting Xu, Xiaoyan Cai, Tingting Dai, Lingxi Wang, Chunying Song, Yushan Li, Fuping Li, Tianqing Meng, Huiqiang Sheng, Xiaolin Yu, Qinghui Zeng, Pi Guo, Xinzong Zhang
BACKGROUNDS: Global fertility rates continue to decline and sperm quality is a prime factor affecting male fertility. Both extreme cold and heat have been demonstrated to be associated with decreased sperm quality, but no epidemiological studies have considered human adaptation to long-term temperature. Our aim was to conduct a multi-center retrospective cohort study to investigate exposure-response relationship between temperature anomaly (TA) that deviate from long-term climate patterns and sperm quality...
March 15, 2024: Heliyon
https://read.qxmd.com/read/38427270/dosing-of-convalescent-plasma-and-hyperimmune-anti-sars-cov-2-immunoglobulins-a-phase-i-ii-dose-finding-study
#19
JOURNAL ARTICLE
Sammy Huygens, Tim Preijers, Francis H Swaneveld, Ilona Kleine Budde, Corine H GeurtsvanKessel, Birgit C P Koch, Bart J A Rijnders
BACKGROUND AND OBJECTIVE: During the COVID-19 pandemic, trials on convalescent plasma (ConvP) were performed without preceding dose-finding studies. This study aimed to assess potential protective dosing regimens by constructing a population pharmacokinetic (popPK) model describing anti-SARS-CoV-2 antibody titers following the administration of ConvP or hyperimmune globulins (COVIg). METHODS: Immunocompromised patients, testing negative for anti-SARS-CoV-2 spike antibodies despite vaccination, received a range of anti-SARS-CoV-2 antibodies in the form of COVIg or ConvP infusion...
March 1, 2024: Clinical Pharmacokinetics
https://read.qxmd.com/read/38425896/asymmetrical-biantennary-glycans-prepared-by-a-stop-and-go-strategy-reveal-receptor-binding-evolution-of-human-influenza-a-viruses
#20
JOURNAL ARTICLE
Shengzhou Ma, Lin Liu, Dirk Eggink, Sander Herfst, Ron A M Fouchier, Robert P de Vries, Geert-Jan Boons
Glycan binding properties of respiratory viruses have been difficult to probe due to a lack of biologically relevant glycans for binding studies. Here, a stop-and-go chemoenzymatic methodology is presented that gave access to a panel of 32 asymmetrical biantennary N -glycans having various numbers of N -acetyl lactosamine (LacNAc) repeating units capped by α2,3- or α2,6-sialosides resembling structures found in airway tissues. It exploits that the branching enzymes MGAT1 and MGAT2 can utilize unnatural UDP-2-deoxy-2-trifluoro- N -acetamido-glucose (UDP-GlcNTFA) as donor...
February 26, 2024: JACS Au
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