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https://www.readbyqxmd.com/read/29663448/seroprevalence-molecular-epidemiology-and-quantitation-of-parvovirus-b19-dna-levels-in-iranian-blood-donors
#1
Maryam Zadsar, Arezoo Aghakhani, Mohammad Banifazl, Monireh Kazemimanesh, Seyed Morteza Tabatabaei Yazdi, Setareh Mamishi, Anahita Bavand, Mona Sadat Larijani, Amitis Ramezani
Human parvovirus B19 (B19) infection is common among blood donors, and healthy blood donors can transmit virus via transfusion. Due to resistance of B19 to viral inactivation methods, there is a potential concern regarding transfusion safety in blood products. We aimed to determine the seroprevalence, molecular epidemiology and quantitation of B19 DNA levels in blood donors in Tehran, Iran. 500 blood donors from Blood Transfusion Research Center were studied. ELISA was used for detection of B19 IgG and IgM and nested PCR was carried out for detection of B19 DNA...
April 16, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29660911/the-effects-of-different-remote-ischemic-conditioning-on-ischemia-induced-failure-of-microvascular-circulation-in-humans
#2
Muzaffer Akkoca, Suzan Emel Usanmaz, Serhat Tokgöz, Cüneyt Köksoy, Emine Demirel-Yilmaz
BACKGROUND: Intermittent ischemia in remote tissues can be applied before ischemic injury, during ischemic injury or at the beginning of reperfusion of an index organ ischemia. The aim of this study was to investigate the effect of Remote Ischemic Conditioning (RIC) of the leg on changes in ischemia-induced the microvascular functions of the arm. MATERIAL AND METHODS: Ischemic microvascular injury was induced by arm ischemia (20 min) and reperfusion in healthy, nonsmoker, male volunteers (ischemia group-ISC, n: 9)...
April 13, 2018: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/29659727/nitrite-circumvents-platelet-resistance-to-nitric-oxide-in-patients-with-heart-failure-preserved-ejection-fraction-and-chronic-atrial-fibrillation
#3
Alessandra Borgognone, Eduard Shantsila, Sophie M Worrall, Eakkapote Prompunt, Thomas Loka, Brodie L Loudon, Myriam Chimen, G Ed Rainger, Janet M Lord, Ashley Turner, Peter Nightingale, Martin Feelisch, Paulus Kirchhof, Gregory Y H Lip, Steve P Watson, Michael P Frenneaux, Melanie Madhani
Aims: Heart failure (HF) is a pro-thrombotic state. Both platelet and vascular responses to nitric oxide (NO) donors are impaired in HF patients with reduced ejection fraction (HFrEF) compared to healthy volunteers (HV) due to scavenging of NO, and possibly also reduced activity of the principal NO sensor, soluble guanylate cyclase (sGC), limiting the therapeutic potential of NO donors as anti-aggregatory agents. Previous studies have shown that nitrite inhibits platelet activation presumptively after its reduction to NO, but the mechanism(s) involved remain poorly characterized...
April 12, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29651422/-aspergillus-induced-superoxide-production-by-cystic-fibrosis-phagocytes-is-associated-with-disease-severity
#4
Shan F Brunel, Janet A Willment, Gordon D Brown, Graham Devereux, Adilia Warris
Aspergillus fumigatus infects up to 50% of cystic fibrosis (CF) patients and may play a role in progressive lung disease. As cystic fibrosis transmembrane conductance regulator is expressed in cells of the innate immune system, we hypothesised that impaired antifungal immune responses play a role in CF-related Aspergillus lung disease. Peripheral blood mononuclear cells, polymorphonuclear cells (PMN) and monocytes were isolated from blood samples taken from CF patients and healthy volunteers. Live-cell imaging and colorimetric assays were used to assess antifungal activity in vitro ...
April 2018: ERJ Open Research
https://www.readbyqxmd.com/read/29645287/seroprevalence-molecular-epidemiology-and-quantitation-of-parvovirus-b19-dna-levels-in-iranian-blood-donors
#5
Maryam Zadsar, Arezoo Aghakhani, Mohammad Banifazl, Monireh Kazemimanesh, Seyed Morteza Tabatabaei Yazdi, Setareh Mamishi, Anahita Bavand, Mona Sadat Larijani, Amitis Ramezani
Human parvovirus B19 (B19) infection is common among blood donors, and healthy blood donors can transmit virus via transfusion. Due to resistance of B19 to viral inactivation methods, there is a potential concern regarding transfusion safety in blood products. We aimed to determine the seroprevalence, molecular epidemiology and quantitation of B19 DNA levels in blood donors in Tehran, Iran. 500 blood donors from Blood Transfusion Research Center were studied. ELISA was used for detection of B19 IgG and IgM and nested PCR was carried out for detection of B19 DNA...
April 12, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29555414/medical-and-ethical-considerations-on-hematopoietic-stem-cells-mobilization-for-healthy-donors
#6
V Moalic-Allain
Hematopoietic stem cell transplantation is a common procedure potentially beneficial to many individuals with cancer, hematological, or inherited disorders, and has highlighted the need of related or unrelated donors to perform allograft. Donation of hematopoietic stem cells, either through bone marrow harvest or peripheral blood stem cell collection, is well-established and widespread. Over the past two decades, the peripheral blood stem cell collection by aphaeresis has become the main source of hematopoietic stem cells for transplantation, due to faster engraftment and practicability and lower risk of relapse for high-risk patients...
March 16, 2018: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
https://www.readbyqxmd.com/read/29544084/long-term-donors-versus-non-donor-men-iron-metabolism-and-the-atherosclerotic-process
#7
Peter Riško, Jan Pláteník, Richard Buchal, Jana Potočková, Pavel J Kraml
BACKGROUND AND AIMS: The increased iron level and the labile iron pool (LIP) in circulating monocytes are connected to a higher frequency of cardiovascular events. METHODS: The study investigates the relationship between LIP in circulating monocytes and markers of iron metabolism and atherosclerosis (inflammation, oxidative stress, endothelial dysfunction and arterial elasticity) in long-term blood donors and non-donor volunteers. RESULTS: We found that donors had significantly higher LIP values than the control group (1...
March 3, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29543828/analytical-validation-of-a-next-generation-sequencing-liquid-biopsy-assay-for-high-sensitivity-broad-molecular-profiling
#8
Vincent Plagnol, Samuel Woodhouse, Karen Howarth, Stefanie Lensing, Matt Smith, Michael Epstein, Mikidache Madi, Sarah Smalley, Catherine Leroy, Jonathan Hinton, Frank de Kievit, Esther Musgrave-Brown, Colin Herd, Katherine Baker-Neblett, Will Brennan, Peter Dimitrov, Nathan Campbell, Clive Morris, Nitzan Rosenfeld, James Clark, Davina Gale, Jamie Platt, John Calaway, Greg Jones, Tim Forshew
Circulating tumor DNA (ctDNA) analysis is being incorporated into cancer care; notably in profiling patients to guide treatment decisions. Responses to targeted therapies have been observed in patients with actionable mutations detected in plasma DNA at variant allele fractions (VAFs) below 0.5%. Highly sensitive methods are therefore required for optimal clinical use. To enable objective assessment of assay performance, detailed analytical validation is required. We developed the InVisionFirst™ assay, an assay based on enhanced tagged amplicon sequencing (eTAm-Seq™) technology to profile 36 genes commonly mutated in non-small cell lung cancer (NSCLC) and other cancer types for actionable genomic alterations in cell-free DNA...
2018: PloS One
https://www.readbyqxmd.com/read/29515997/hepatitis-b-virus-blood-screening-need-for-reappraisal-of-blood-safety-measures
#9
REVIEW
Daniel Candotti, Syria Laperche
Over the past decades, the risk of HBV transfusion-transmission has been steadily reduced through the recruitment of volunteer donors, the selection of donors based on risk-behavior evaluation, the development of increasingly more sensitive hepatitis B antigen (HBsAg) assays, the use of hepatitis B core antibody (anti-HBc) screening in some low-endemic countries, and the recent implementation of HBV nucleic acid testing (NAT). Despite this accumulation of blood safety measures, the desirable zero risk goal has yet to be achieved...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/29513667/zika-virus-epidemiology-in-bolivia-a-seroprevalence-study-in-volunteer-blood-donors
#10
Paola Mariela Saba Villarroel, Elif Nurtop, Boris Pastorino, Yelin Roca, Jan Felix Drexler, Pierre Gallian, Thomas Jaenisch, Isabelle Leparc-Goffart, Stéphane Priet, Laetitia Ninove, Xavier de Lamballerie
BACKGROUND: Zika virus (ZIKV), was widely reported in Latin America and has been associated with neuropathologies, as microcephaly, but only few seroprevalence studies have been published to date. Our objective was to determine the seroprevalence amongst Bolivian blood donors and estimate the future potential circulation of the virus. METHODOLOGY: A ZIKV seroprevalence study was conducted between December 2016 and April 2017 in 814 asymptomatic Bolivian volunteer blood donors residing in various eco-environments corresponding to contrasting entomological activities...
March 7, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29497381/delipidation-of-plasma-has-minimal-effects-on-human-butyrylcholinesterase
#11
Seda Onder, Ozden Tacal, Oksana Lockridge
Human butyrylcholinesterase (BChE) is purified in large quantities from Cohn fraction IV-4 to use for protection against the toxicity of chemical warfare agents. Small scale preliminary experiments use outdated plasma from the American Red Cross as the starting material for purifying BChE (P06276). Many of the volunteer donor plasma samples are turbid with fat, the donor having eaten fatty food before the blood draw. The turbid fat interferes with enzyme assays performed in the spectrophotometer and with column chromatography...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29475179/comprehensive-characteristics-of-the-anticoagulant-activity-of-dabigatran-in-relation-to-its-plasma-concentration
#12
Willemijn J Comuth, Linda Ø Henriksen, Daan van de Kerkhof, Steen E Husted, Steen D Kristensen, Moniek P M de Maat, Anna-Marie B Münster
BACKGROUND: Issues with laboratory measurement of dabigatran include: 1. Do coagulation assays reflect dabigatran plasma concentrations? 2. Do samples from patients treated with dabigatran have the same coagulability as dabigatran-spiked samples from healthy volunteers? 3. What is the long-term stability of dabigatran after storage at -80 °C? This study aims to evaluate these questions. MATERIALS AND METHODS: Ecarin chromogenic assay (ECA), a laboratory-developed diluted thrombin time (LD-dTT), prothrombin time (PT) and activated partial thromboplastin time (APTT) and ROTEM® were used to measure dabigatran anticoagulant activity and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to measure dabigatran plasma concentrations...
February 17, 2018: Thrombosis Research
https://www.readbyqxmd.com/read/29472138/understanding-the-underlying-motives-and-intention-among-indian-blood-donors-towards-voluntary-blood-donation-a-cross-sectional-study
#13
S Saha, B Chandra
OBJECTIVES: The present study aims to fill the gap in the literature by conducting a comprehensive research on Indian donor's intention towards voluntary blood donation in India. The study attempts to conceptualize and validate an integrative framework incorporating voluntary function inventory (VFI) in the theory of planned behaviour (TPB) model with the purpose tomeasure the voluntary blood donation intention. MATERIAL AND METHODS: Structural equation modeling (SEM) has been used to rigorously test the hypothesized interrelationships among the underlying motives influencing voluntary blood donation intention...
February 19, 2018: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
https://www.readbyqxmd.com/read/29443378/hepatitis-c-virus-e2-envelope-glycoprotein-induces-an-immunoregulatory-phenotype-in-macrophages
#14
Young-Chan Kwon, Keith Meyer, Guangyong Peng, Soumya Chatterjee, Daniel F Hoft, Ranjit Ray
A comprehensive strategy to control HCV infection needs a vaccine. Our phase I study with recombinant HCV E1/E2 EnvGPs as a candidate vaccine did not induce a strong immune response in volunteers. We analyzed the interactions of HCV EnvGPs with human monocyte derived macrophages as antigen presenting cells. HCV E2 induced immune regulatory cytokine IL-10 and sCD163 protein expression in macrophages from 7 of 9 blood donors tested. Further, HCV E2 enhanced Stat3 and suppressed Stat1 activation, reflecting macrophage polarization towards M2 phenotype...
February 14, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29434980/augmentation-of-antibody-dependent-cellular-cytotoxicity-with-defucosylated-monoclonal-antibodies-in-patients-with-gi-tract-cancer
#15
Takahiro Nakajima, Hirokazu Okayama, Mai Ashizawa, Masaru Noda, Keita Aoto, Motonobu Saito, Tomoyuki Monma, Shinji Ohki, Masahiko Shibata, Seiichi Takenoshita, Koji Kono
Enhancement of antibody-dependent cellular cytotoxicity (ADCC) with some modalities may be a promising approach to enhance the efficacy of therapeutic monoclonal antibodies (mAbs). It has previously been demonstrated that the removal of fucose from antibody oligosaccharides (defucosylation) leads to augmentation of ADCC activity. To establish clinically relevant evidence of this procedure, the present study evaluated trastuzumab- and cetuximab-mediated ADCC by comparing defucosylated mAbs with conventional mAbs using peripheral blood mononuclear cells (PBMCs)...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29424063/it-is-only-fair-blood-donors-are-more-sensitive-to-violations-of-fairness-norms-than-nondonors-converging-psychometric-and-ultimatum-game-evidence
#16
E Ferguson, C Lawrence
BACKGROUND AND OBJECTIVES: The design of effective donor recruitment campaigns requires an accurate understanding of donor motivations. This requires cross-validation of theoretically derived, psychometrically assessed motivations with behavioural preferences. Theoretical models suggest that blood donors should be more sensitive than nondonors to violations of fairness norms. Specifically, active blood donors, compared to nondonors, should endorse beliefs of reciprocal fairness, norms of both positive and negative reciprocity and reject more unfair offers in a behavioural economic game (the ultimatum game)...
February 9, 2018: Vox Sanguinis
https://www.readbyqxmd.com/read/29419875/-association-of-polymorphisms-of-kir2dl4-gene-with-leukemia-among-ethnic-hans-from-southern-china
#17
Rui Chen, Zhihui Deng
OBJECTIVE To assess the association of polymorphisms of KIR2DL4 gene with leukemia among ethnic Hans from southern China. METHODS A total of 590 leukemia patients were recruited, which included 283 cases of acute lymphoblastic leukemia (ALL), 277 cases of acute myeloid leukemia (AML), and 80 cases of chronic myeloid leukemia (CML). Meanwhile, 306 healthy controls were randomly selected from volunteer blood donors. Both groups were subjected to sequence-based typing of the entire coding sequence of the KIR2DL4 gene using an in-house assay...
February 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/29410372/addressing-ethical-and-procedural-principles-for-unrelated-allogeneic-hematopoietic-progenitor-cell-donation-in-a-changing-medical-environmentbscomment
#18
Suzanna M van Walraven, Torstein Egeland, Veronica Borrill, Grazia Nicoloso-de Faveri, Gabi Rall, Jeff Szer
No abstract text is available yet for this article.
February 2, 2018: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/29377063/measurement-of-typhim-vi-antibodies-can-be-used-to-assess-adaptive-immunity-in-patients-with-immunodeficiency
#19
Caroline Evans, Elizabeth Bateman, Rachael Steven, Mark Ponsford, Alice Cullinane, Claire Shenton, Gillian Duthie, Chris Conlon, Stephen Jolles, Aarnoud P Huissoon, Hilary J Longhurst, Tasneem Rahman, Chris Scott, Gregg Wallis, Stephen Harding, Antony R Parker, Berne L Ferry
Vaccine specific antibody responses are essential in the diagnosis of antibody deficiencies. Responses to Pneumovax II are used to assess the response to polysaccharide antigens but interpretation may be complicated. Typhim Vi®, a polysaccharide vaccine for Salmonella typhoid fever, maybe an additional option for assessing humoral responses in patients suspected of having an immunodeficiency. Here we report a UK multicentre study describing the analytical and clinical performance of a Typhi Vi IgG ELISA calibrated to an affinity purified Typhi Vi IgG preparation...
January 29, 2018: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/29370542/capacity-of-ceruloplasmin-to-scavenge-products-of-the-respiratory-burst-of-neutrophils-is-not-altered-by-the-products-of-reactions-catalyzed-by-myeloperoxidase
#20
Alexey Sokolov, Valeria A Kostevich, Elena Yu Varfolomeeva, Daria V Grigorieva, Irina V Gorudko, Stanislav O Kozlov, Igor V Kudryavtsev, Elena V Mikhalchik, Michael V Filatov, Sergey N Cherenkevich, Oleg M Panasenko, Juergen Arnhold, Vadim B Vasilyev
Ceruloplasmin (CP) is a copper-containing ferroxidase of blood plasma, acting as an acute phase reactant during inflammation. The effect of oxidative modification of CP induced by oxidants produced by myeloperoxidase, such as HOCl, HOBr and HOSCN, on its spectral, enzymatic and anti-inflammatory properties was studied. We monitored chemiluminescence of lucigenin and luminal along with fluorescence of hydroethidine and scopoletin to assay the inhibition by CP of the neutrophilic respiratory burst induced by phorbol 12-myristate 13-acetate (PMA) or formyl-methionyl-leucyl-phenylalanine (fMLP)...
January 25, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
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