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volunteer blood donor

Young-Chan Kwon, Keith Meyer, Guangyong Peng, Soumya Chatterjee, Daniel F Hoft, Ranjit Ray
A comprehensive strategy to control HCV infection needs a vaccine. Our phase I study with recombinant HCV E1/E2 EnvGPs as a candidate vaccine did not induce a strong immune response in volunteers. We analyzed the interactions of HCV EnvGPs with human monocyte derived macrophages as antigen presenting cells. HCV E2 induced immune regulatory cytokine IL-10 and sCD163 protein expression in macrophages from 7 of 9 blood donors tested. Further, HCV E2 enhanced Stat3 and suppressed Stat1 activation, reflecting macrophage polarization towards M2 phenotype...
February 14, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Takahiro Nakajima, Hirokazu Okayama, Mai Ashizawa, Masaru Noda, Keita Aoto, Motonobu Saito, Tomoyuki Monma, Shinji Ohki, Masahiko Shibata, Seiichi Takenoshita, Koji Kono
Enhancement of antibody-dependent cellular cytotoxicity (ADCC) with some modalities may be a promising approach to enhance the efficacy of therapeutic monoclonal antibodies (mAbs). It has previously been demonstrated that the removal of fucose from antibody oligosaccharides (defucosylation) leads to augmentation of ADCC activity. To establish clinically relevant evidence of this procedure, the present study evaluated trastuzumab- and cetuximab-mediated ADCC by comparing defucosylated mAbs with conventional mAbs using peripheral blood mononuclear cells (PBMCs)...
February 2018: Oncology Letters
E Ferguson, C Lawrence
BACKGROUND AND OBJECTIVES: The design of effective donor recruitment campaigns requires an accurate understanding of donor motivations. This requires cross-validation of theoretically derived, psychometrically assessed motivations with behavioural preferences. Theoretical models suggest that blood donors should be more sensitive than nondonors to violations of fairness norms. Specifically, active blood donors, compared to nondonors, should endorse beliefs of reciprocal fairness, norms of both positive and negative reciprocity and reject more unfair offers in a behavioural economic game (the ultimatum game)...
February 9, 2018: Vox Sanguinis
Rui Chen, Zhihui Deng
OBJECTIVE To assess the association of polymorphisms of KIR2DL4 gene with leukemia among ethnic Hans from southern China. METHODS A total of 590 leukemia patients were recruited, which included 283 cases of acute lymphoblastic leukemia (ALL), 277 cases of acute myeloid leukemia (AML), and 80 cases of chronic myeloid leukemia (CML). Meanwhile, 306 healthy controls were randomly selected from volunteer blood donors. Both groups were subjected to sequence-based typing of the entire coding sequence of the KIR2DL4 gene using an in-house assay...
February 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
S M van Walraven, T Egeland, V Borrill, G Nicoloso-de Faveri, G Rall, J Szer
Transplantation of allogeneic haematopoietic progenitor cells (HPC), as part of a potential life-saving treatment for haematological disorders, is a well-established medical procedure. Due to the efforts of the World Marrow Donor Association (WMDA) and other professional medical and scientific organisations, donation and collection of HPC from adult volunteer donors has become suitably regulated and standardized. Ongoing changes in the treatment of haematological disorders however, have led to a practice where organisations providing HPC from volunteer donors are confronted with requests for multiple or other than standard bone marrow or peripheral blood stem cell donations...
February 1, 2018: Biology of Blood and Marrow Transplantation
Caroline Evans, Elizabeth Bateman, Rachael Steven, Mark Ponsford, Alice Cullinane, Claire Shenton, Gillian Duthie, Chris Conlon, Stephen Jolles, Aarnoud P Huissoon, Hilary J Longhurst, Tasneem Rahman, Chris Scott, Gregg Wallis, Stephen Harding, Antony R Parker, Berne L Ferry
Vaccine specific antibody responses are essential in the diagnosis of antibody deficiencies. Responses to Pneumovax II are used to assess the response to polysaccharide antigens but interpretation may be complicated. Typhim Vi®, a polysaccharide vaccine for Salmonella typhoid fever, maybe an additional option for assessing humoral responses in patients suspected of having an immunodeficiency. Here we report a UK multicentre study describing the analytical and clinical performance of a Typhi Vi IgG ELISA calibrated to an affinity purified Typhi Vi IgG preparation...
January 29, 2018: Clinical and Experimental Immunology
Alexey Sokolov, Valeria A Kostevich, Elena Yu Varfolomeeva, Daria V Grigorieva, Irina V Gorudko, Stanislav O Kozlov, Igor V Kudryavtsev, Elena V Mikhalchik, Michael V Filatov, Sergey N Cherenkevich, Oleg M Panasenko, Juergen Arnhold, Vadim B Vasilyev
Ceruloplasmin (CP) is a copper-containing ferroxidase of blood plasma, acting as an acute phase reactant during inflammation. The effect of oxidative modification of CP induced by oxidants produced by myeloperoxidase, such as HOCl, HOBr and HOSCN, on its spectral, enzymatic and anti-inflammatory properties was studied. We monitored chemiluminescence of lucigenin and luminal along with fluorescence of hydroethidine and scopoletin to assay the inhibition by CP of the neutrophilic respiratory burst induced by phorbol 12-myristate 13-acetate (PMA) or formyl-methionyl-leucyl-phenylalanine (fMLP)...
January 25, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Noor Rehman, M Bilal Orakzai, Azam Hayat, Sadiq Azam, Bashir Ahmad, Ibrar Khan, Zermina Zeb
The current study was designed in order to elucidate the most sensitive method for daily practice as well as to evaluate the risk factors for HCV infection associated with blood transfusion in District Peshawar. A total of 1400 healthy volunteer blood donors were tested for Anti-HCV. A questionnaire was used to evaluate the risk factors. Initial testing of all blood samples was done by Immuno Chromatographic Technique (ICT) and confirmed by micro particle enzyme immunoassay (MEIA) and Enzyme Linked Immunosorbent Assay (ELISA)...
January 2018: Pakistan Journal of Pharmaceutical Sciences
Yushe Dang, Zina J Rutnam, Gregory Dietsch, Hailing Lu, Yi Yang, Robert Hershberg, Mary L Disis
Myeloid-derived suppressor cells (MDSCs) accumulate in tumors and the peripheral blood of cancer patients and demonstrate cancer-promoting activity across multiple tumor types. A limited number of agents are known to impact MDSC activity. TLR8 is expressed in myeloid cells. We investigated expression of TLR8 on MDSC and the effect of a TLR8 agonist, motolimod, on MDSC survival and function. TLR8 was highly expressed in monocytic MDSC (mMDSC) but absent in granulocytic MDSC (gMDSC). Treatment of human PBMC with motolimod reduced the levels of mMDSC in volunteers and cancer donors versus control (P < 0...
January 2018: Journal of Leukocyte Biology
Mahboubeh Rasouli, Mojgan Pourmokhtar, Shaghayegh Sarkardeh
Background: Development of locally-derived hematological reference intervals is necessary for improving the quality of health care and clinical trials. However hematological reference intervals are affected by several variables including age, gender and environmental factors. Therefore this study was conducted to determine the gender and age-specific hematological reference intervals of healthy Iranian blood donors. Materials and Methods: Selected hematological indices of 394 healthy blood donor volunteers, donating blood at Tehran Blood Transfusion Center were analyzed...
October 1, 2017: International Journal of Hematology-oncology and Stem Cell Research
Anne F Kristensen, Søren R Kristensen, Ursula Falkmer, Anna-Marie B Münster, Shona Pedersen
BACKGROUND: The Calibrated Automated Thrombography (CAT) is an in vitro thrombin generation (TG) assay that holds promise as a valuable tool within clinical diagnostics. However, the technique has a considerable analytical variation, and we therefore, investigated the analytical and between-subject variation of CAT systematically. Moreover, we assess the application of an internal standard for normalization to diminish variation. METHODS: 20 healthy volunteers donated one blood sample which was subsequently centrifuged, aliquoted and stored at -80 °C prior to analysis...
January 16, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Rachel Culp-Hill, Connie Zheng, Julie A Reisz, Keith Smith, Angela Rachubinski, Travis Nemkov, Eric Butcher, Ross Granrath, Kirk C Hansen, Joaquín M Espinosa, Angelo D'Alessandro
Red blood cells (RBCs) are the most abundant cell in the human body. During their ∼120-day life span in the circulatory system, RBCs release oxygen to all human tissues while being exposed to tissue metabolic activity. Owing to the relative simplicity of their intrinsic metabolism and the abundance of metabolite transporters in RBC membranes, the metabolism of mature erythrocytes indirectly mirrors systemic metabolic homeostasis and its alterations as a function of physiological factors, such as aging. Trisomy 21 (T21), the etiological factor of Down syndrome (DS), has been shown to cause chronic autoinflammation, promoting alterations in RBC life span, size (macrocytosis), and redox homeostasis...
December 26, 2017: Blood Advances
Abdulrahman Alshalani, Anita Howell, Jason P Acker
Several factors have been proposed to influence the red blood cells storage lesion including storage duration, blood component manufacturing methodology, and donor characteristics [1,18]. The objectives of this study were to determine the impact of manufacturing method and donor characteristics on water permeability and membrane quality parameters. Red blood cell units were obtained from volunteer blood donors and grouped according to the manufacturing method and donor characteristics of sex and age. Membrane water permeability and membrane quality parameters, including deformability, hemolysis, osmotic fragility, hematologic indices, supernatant potassium, and supernatant sodium, were determined at three testing points of day 5 ± 2, day 21, and day 42...
December 22, 2017: Cryobiology
X X Liu, D D Li, H L Li, L A Hou, Z J Liu, H Y Yang, L Qiu
Objective: To explore the distribution of ABO blood group in the healthy population in the Ali area of Tibet, and to analyze the difference of blood group distribution between the Tibetan population in Ali and the Tibet Tibetan population. Methods: The blood distribution of 509 apparent healthy volunteers of Tueti County and Gal County, Tibet, which were randomly selected from September to November in 2016; 137 Tibetan blood donors, from 2016 September to2017 July and 84 Tibetan blood donors from 2015 August to 2017 July was analyzed retrospectively...
December 12, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
V V Masljakov, O A Suhanova, V G Barsukov, K G Kurkin, S A Suhanov
The purpose: research objective: to study influence of electromagnetic oscillations of millimetric range on rheological properties of blood at patients with chipped and cut wounds of a breast for the purpose of their correction. Methods. For the solution of a research objective we have carried out studying of changes of rheological properties of blood at the 22nd patient with the getting chipped and cut wounds of a breast without internal injury during the next postoperative period. All patient has executed primary surgical processing and drainage of a pleural cavity...
April 2017: Patologicheskaia Fiziologiia i èksperimental'naia Terapiia
Janusz Klatka, Ewelina Grywalska, Anna Hymos, Ewelina Krasowska, Michał Mielnik, Dorota Siwicka-Gieroba, Justyna Markowicz, Piotr Trojanowski, Witold Olszański, Jacek Roliński
Introduction: Tumours connected with head and neck comprise about 5% of all tumours. The most frequent histological type of laryngeal carcinoma is squamous cell carcinoma. Different research projects suggest that the role of T lymphocytes might be significant in tumour development. iNKT cells are a new subpopulation of T cells and show cytotoxic activity against tumours. iNKT cells participate in modulating the function of other cells which have anti-tumour properties and secrete cytokines, which have pro-inflammatory and anti-inflammatory effects...
2017: Central-European Journal of Immunology
Eva-Maria Merz, Katja van den Hurk, Wim L A M de Kort
INTRODUCTION: In the Netherlands, there is a constant shortage in donor organs, resulting in long waiting lists. The decision to register as organ donor is associated with several demographic, cultural, and personal factors. Previous research on attitudes and motivations toward blood and organ donations provided similar results. RESEARCH QUESTION: The current study investigated demographic, cultural, and personal determinants of organ donation registration among current Dutch blood donors...
September 2017: Progress in Transplantation
S Bakrim, A Ouarour, K Jaidann, M Benajiba, A Masrar
BACKGROUND: Blood donation in Morocco and more particularly in the northwest region is carried out without prior determination of the pre-donation hemoglobin. In addition, we note the lack of scientific research that reports data on the red blood cells, leukocytes and platelet lines in donated blood at the regional or even national level. AIMS: To study hemogram profile in blood donors taken from the Northwest region of Morocco in order to provide decision makers of the National Center of Blood Transfusion and Hematology with valid scientific arguments to complete the criteria to donate whole blood, by the hemogram...
November 13, 2017: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
Li-Xin Jiao, Jing-Ying Zhang, Lin Chen, Xiao-Li Yu, Yu Han, Fan Yang, Jiang-Hong Yu, Ling-Ling Liu, Yue Lang
OBJECTIVE: To study serologic and gene characteristics of the B(A) blood group of blood donation volunteers in Jilin Province, China. METHODS: ABO subgroups were identified by standard serologic techniques in ABO typing discrepancy samples from all donors at the Jilin Blood Center (410,354 non-repeat donors). DNA (deoxyribonucleic acid) was collected from each sample and PCR (polymerase chain reaction) was used to sequence exons 6 and 7 and intron 6, part 5 from the ABO subgroup samples...
September 11, 2017: Transfusion and Apheresis Science
Pansakorn Tanratana, Paul Ellery, Pamela Westmark, Alan E Mast, John P Sheehan
OBJECTIVE: Combined oral contraceptives induce a reversible hypercoagulable state with an enhanced risk of venous thromboembolism, but the underlying mechanism(s) remain unclear. Subjects on combined oral contraceptives also demonstrate a characteristic resistance to APC (activated protein C) in the thrombin generation assay. Here, we report the potential role of plasma factor IXa (FIXa) as a mechanism for hormone-induced systemic hypercoagulability. APPROACH AND RESULTS: A novel assay was used to determine FIXa activity in plasma samples from volunteer blood donors...
November 2, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
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