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https://www.readbyqxmd.com/read/29053932/the-patterns-of-in-vitro-cell-death-and-inflammatory-cytokines-induced-by-distinct-bcg-vaccine-strains-are-differentially-induced-in-human-mononuclear-cells
#1
C Ponte, M Hacker, M Moraes, L Castello-Branco, F Silva, P Antas
Tuberculosis (TB) remains among the world's leading cause of mortality. For its control, studies of TB vaccines are needed. Since live-attenuated Mycobacterium bovis bacillus Calmette-Guérin (BCG) is the only TB vaccine currently in use, studies on the protective role of BCG are required. In this study, we analyzed host cells purified directly from whole blood of human immunodeficiency virus (HIV)-negative volunteers, comprising adult healthy donors (HD) and neonates (umbilical cord bloods, UCB), with the aim to directly compare in vitro immune responses with distinct BCG strains in human mononuclear cells...
October 20, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29039348/the-impact-of-agonists-and-antagonists-of-tlr3-and-tlr9-on-concentrations-of-il-6-il10-and-sil-2r-in-culture-supernatants-of-peripheral-blood-mononuclear-cells-derived-from-patients-with-systemic-lupus-erythematosus
#2
Agnieszka Klonowska-Szymczyk, Liliana Kulczycka-Siennicka, Tadeusz Robak, Piotr Smolewski, Barbara Cebula-Obrzut, Ewa Robak
Toll-like receptors (TLR), especially TLR3, 7 and 9, play an important role in the pathogenesis of systemic lupus erythematosus (SLE). In our study blood was collected from 16 patients with SLE and from 8 healthy volunteers. Concentrations of IL-6, IL-10 and sIL-2R were measured by ELISA in mononuclear cell culture supernatant after 24 hours of stimulation by agonists and antagonists of TLR3 and 9 (for TLR3-poli I/C, resveratrol and for TLR9-ODN2006, IRS 945). Stimulation of TLR9 by ODN2006 led to an increase of IL-6 concentration in cell culture supernatants from the cells of healthy volunteers compared with unstimulated cells from controls...
October 13, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/29039102/analysis-of-pro-inflammatory-cytokine-and-type-ii-interferon-induction-by-nanoparticles
#3
Timothy M Potter, Barry W Neun, Jamie C Rodriguez, Anna N Ilinskaya, Marina A Dobrovolskaia
Cytokines, chemokines, and interferons are released by the immune cells in response to cellular stress, damage and/or pathogens, and are widely used as biomarkers of inflammation. Certain levels of cytokines are needed to stimulate an immune response in applications such as vaccines or immunotherapy where immune stimulation is desired. However, undesirable elevation of cytokine levels, as may occur in response to a drug or a device, may lead to severe side effects such as systemic inflammatory response syndrome or cytokine storm...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29036415/transfusion-transmissible-infections-among-walk-in-blood-donors-at-kisumu-regional-blood-transfusion-centre-kisumu-county-kenya-2015
#4
Dominic Wamamba, Dickens Onyango, Elvis Oyugi, Evalyne Kanyina, Mark Obonyo, Jane Githuku, James Ransom
Background: Transfusion transmissible infections (TTIs) are threats to blood safety. This study investigated TTIs among volunteer blood donors at the Kisumu Regional Blood Transfusion Centre (KRBTC) in Kenya. Methods: We performed a retrospective record review of blood donor registers at KRBTC, 2015. Walk-in donors accepted for donation were analyzed to determine prevalence of human immunodeficiency virus (HIV), hepatitis B (HBV), hepatitis C (HCV), and syphilis...
September 23, 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/29029634/is-mmtv-associated-with-human-breast-cancer-maybe-but-probably-not
#5
Raisa Perzova, Lynn Abbott, Patricia Benz, Steve Landas, Seema Khan, Jordan Glaser, Coleen K Cunningham, Bernard Poiesz
BACKGROUND: Conflicting results regarding the association of MMTV with human breast cancer have been reported. Published sequence data have indicated unique MMTV strains in some human samples. However, concerns regarding contamination as a cause of false positive results have persisted. METHODS: We performed PCR assays for MMTV on human breast cancer cell lines and fresh frozen and formalin fixed normal and malignant human breast epithelial samples. Assays were also performed on peripheral blood mononuclear cells from volunteer blood donors and subjects at risk for human retroviral infections...
October 13, 2017: Virology Journal
https://www.readbyqxmd.com/read/29028283/implementation-of-buffy-coat-derived-pooled-platelet-concentrates-for-internal-quality-control-of-light-transmission-aggregometry-a-proof-of-concept-study
#6
F Prüller, K Rosskopf, H Mangge, E Mahla, D von Lewinski, E C Weiss, A Riegler, D Enko
BACKGROUND: Standardized commercially available control material for internal quality control (IQC) of light transmission aggregometry (LTA) is still lacking. Moreover, the provision of normal blood donors to provide fresh platelets is difficult in small laboratories, where "volunteers" may be of short supply. OBJECTIVES: To evaluate the implementation of buffy-coat-derived pooled platelet concentrates (PCs) for IQC material of LTA. METHODS: We used buffy-coat-derived pooled PCs from the blood bank as IQC material for LTA...
October 13, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28992132/dna-strand-breaks-in-peripheral-blood-leucocytes-of-polish-blood-donors
#7
Malgorzata M Dobrzynska, Krzysztof A Pachocki, Katarzyna Owczarska
Knowledge about the basal level of DNA damage in leucocytes of healthy control populations is essential before estimation of the effects of exposure to external agents in biomonitoring studies. The aim of this study was to analyse the effects of some lifestyle factors on baseline DNA damage in leucocytes of humans. The material consisted of the peripheral blood from 276 healthy volunteer blood donors. In addition to the standard blood donation questionnaire, they were asked about age, gender, occupation, radiological history, smoking habit, alcohol consumption, medicine use and pet ownership...
September 13, 2017: Mutagenesis
https://www.readbyqxmd.com/read/28969566/herbal-mixture-adsorbed-to-polyethylene-glycol-microspheres-induces-apoptotic-effects-on-breast-cancer-cells
#8
Aliny Aparecida Lopes Ribeiro, Fabiana Helen da Silva, Aron Carlos de Melo Cotrim, Alessandra Lima Deluque, Patrícia Gelli Feres de Marchi, Danny Laura Gomes Fagundes, Claudia de Sousa Pereira, Eduardo Luzía França, Adenilda Cristina Honorio-França
The combination of medicinal plants and polymeric matrices raised the possibility of obtaining new drugs to treat a number of diseases, including cancer. Breast cancer is one of the most frequent diseases among women, but the effective treatments is still a challenge. Thus, this study investigated the effect of herbal mixture adsorbed to PEG microspheres on MCF-7 human breast cancer cells and these cells in co-culture of blood MN cells. The MCF-7 cells and the blood mononuclear [MN] Cells from volunteer donors...
October 2, 2017: Current Drug Delivery
https://www.readbyqxmd.com/read/28967462/myophosphorylase-pygm-mutations-determined-by-next-generation-sequencing-in-a-cohort-from-turkey-with-mcardle-disease
#9
Güldal Inal-Gültekin, Bahar Toptaş-Hekimoğlu, Zeliha Görmez, Özlem Gelişin, Hacer Durmuş, Bekir Ergüner, Hüseyin Demirci, Mahmut Ş Sağıroğlu, Yeşim Parman, Feza Deymeer, Hülya Yılmaz-Aydoğan, Sadrettin Pençe, Can Ebru Bekircan-Kurt, Ersin Tan, Sevim Erdem-Özdamar, Duran Üstek, Urs Giger, Oğuz Öztürk, Piraye Serdaroğlu-Oflazer
This study aimed to identify PYGM mutations in patients with McArdle disease from Turkey by next generation sequencing (NGS). Genomic DNA was extracted from the blood of the McArdle patients (n = 67) and unrelated healthy volunteers (n = 53). The PYGM gene was sequenced with NGS and the observed mutations were validated by direct Sanger sequencing. A diagnostic algorithm was developed for patients with suspected McArdle disease. A total of 16 deleterious PYGM mutations were identified, of which 5 were novel, including 1 splice-site donor, 1 frame-shift, and 3 non-synonymous variants...
June 16, 2017: Neuromuscular Disorders: NMD
https://www.readbyqxmd.com/read/28962103/erythrocyte-sedimentation-rate-and-fibrinogen-concentration-of-whole-blood-influences-the-cellular-composition-of-platelet-rich-plasma-obtained-from-centrifugation-methods
#10
Wenjing Yin, Zhengliang Xu, Jiagen Sheng, Xuetao Xie, Changqing Zhang
Erythrocyte sedimentation rate (ESR), which reflects the sedimentation rate of platelets, leukocytes and erythrocytes in response to centrifugal force, may influence the cellular composition of platelet-rich plasma (PRP) obtained via centrifugation methods. However, no relevant studies have substantiated this. In the present study, blood was collected from 40 healthy volunteers and used to prepare PRP with two plasma-based preparation systems [YinPRP and Plasma Rich in Growth Factor (PRGF) systems] and two buffy coat-based systems (RegenPRP and WEGOPRP systems) in a single-donor model...
September 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28960897/a-uniform-shear-rate-microfluidic-bioreactor-for-real-time-study-of-proplatelet-formation-and-rapidly-released-platelets
#11
Andres F Martinez, Richard D McMahon, Marc Horner, William M Miller
Platelet transfusions, with profound clinical importance in blood clotting and wound healing, are entirely derived from human volunteer donors. Hospitals rely on a steady supply of donations, but these methods are limited by a 5-day shelf life, the potential risk of contamination, and differences in donor/recipient histocompatibility. These challenges invite the opportunity to generate platelets ex vivo. Although much progress has been made in generating large numbers of culture-derived megakaryocytes (Mks, the precursor cells to platelets), stimulating a high percentage of Mks to undergo platelet release remains a major challenge...
September 27, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28952291/high-resolution-hla-a-typing-in-normal-iranian-population
#12
Azadeh Hadadianpour, Mohammad Hasan Samiee Aref, Sirous Zeinali
Background: Human leukocyte antigen (HLA) gene is a highly polymorphic region. HLA typing is required to match patients and donors for transplantation; therefore, development of HLA registries is necessary for finding HLA match donors. HLA system is highly informative, and numerous studies have been conducted on HLA allele distribution in different populations. Methods: In this study, 100 unrelated Iranian individuals were typed for HLA-A locus using sequence-based typing method...
September 27, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28928957/update-on-umbilical-cord-blood-transplantation
#13
REVIEW
Karen Ballen
Allogeneic hematopoietic cell transplant is a curative procedure for many patients with leukemia, lymphoma, myelodysplasia, myeloproliferative neoplasms, and genetic disorders. Umbilical cord blood transplantation is a graft source for patients who do not have a matched donor in their family or in the unrelated registry. It is particularly difficult for Black, Hispanic, and White patients of non-Western European background to find fully matched adult volunteer donors. An estimated 700,000 umbilical cord blood units have been donated for public use, and over 40,000 umbilical cord blood transplantations have been performed...
2017: F1000Research
https://www.readbyqxmd.com/read/28921551/zika-virus-rna-detection-in-asymptomatic-blood-donors-during-an-outbreak-in-the-northeast-region-of-s%C3%A3-o-paulo-state-brazil-2016
#14
Svetoslav N Slavov, Marta R Hespanhol, Evandra S Rodrigues, José E Levi, Eugênia M A Ubiali, Dimas T Covas, Simone Kashima
BACKGROUND: In 2015, there was a large Zika virus (ZIKV) outbreak in Brazil. The proportion of asymptomatic infections is very high, and it is possible for transfusion-transmitted ZIKV (TT-ZIKV) infection to occur. The prevalence of asymptomatic ZIKV infection among Brazilian blood donors during this epidemic outbreak is unknown. STUDY DESIGN AND METHODS: Plasma samples obtained between October 2015 and May 2016 from 1393 volunteer blood donors were tested for ZIKV RNA...
September 16, 2017: Transfusion
https://www.readbyqxmd.com/read/28905197/systemic-glyceryl-trinitrate-reduces-anal-sphincter-tone-is-there-a-therapeutic-indication
#15
C Connolly, S Tierney, P Grace
BACKGROUND: Nitric oxide (NO) has diverse roles as a biological messenger. [1] Topically applied nitrate donors cause relaxation of the internal anal sphincter (IAS) and facilitate healing of anal fissures [2,3]. Systemic nitrates are commonly used for the treatment of ischaemic heart disease, yet the effects of systemically administered nitrates on the smooth muscle of the IAS are unknown. AIM: Our aim was to test the hypothesis that systemically administered nitrates at a normal dose, cause inhibition of anal sphincter activity...
September 13, 2017: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/28883026/group-o-low-titre-only-emergency-donor-panels-for-small-combat-teams
#16
Heidi Doughty, A Maguire, G Fitchett, P Parker
INTRODUCTION: Military elements increasingly operate in small teams in remote areas with no immediate blood product support. Planners and operators may endorse collection of fresh whole blood from pretested donors in emergency situations. The biggest risk of transfusion is the accidental use of ABO incompatible blood which can be fatal. The risk may be mitigated by using only group O LOw (OLO) titre donors with plasma containing low levels of the naturally occurring antibody to group A and B red cells...
September 6, 2017: Journal of the Royal Army Medical Corps
https://www.readbyqxmd.com/read/28805478/production-of-platelet-rich-plasma-gel-from-elderly-patients-under-antithrombotic-drugs-perspectives-in-chronic-wounds-care
#17
M Velier, J Magalon, A Daumas, M Cassar, P Francois, A Ghazouane, C Philandrianos, B Bertrand, C Frere, D Bernot, P Villani, F Dignat George, F Sabatier
Platelet-Rich Plasma (PRP) is an autologous biological therapy obtained by centrifuging the patient's own blood to concentrate platelets. The addition of autologous thrombin and calcium chloride to PRP allows the production of a semi-solid form called PRP gel. PRP gel is increasingly used in a variety of tissue defects and predominantly in the management of non-healing chronic wounds. The topical application of PRP gel seems promising due to the capability of platelets to store and secrete growth factors (GF), fibrin and cytokines, which are essentials for wound healing...
August 14, 2017: Platelets
https://www.readbyqxmd.com/read/28803004/-just-blood-donors-a-study-on-the-multi-affiliations-of-blood-donors
#18
Sara Alfieri, Maura Pozzi, Elena Marta, Vincenzo Saturni, Giovanni Aresi, Paolo Guiddi
BACKGROUND: The present work proposes to explore a phenomenon well known in the world of blood donation, but little explored by literature: multi-affiliations. By that term, in this paper we mean blood donors' engagement in multiple associations of various natures (donation, recreation, sports, etc.) simultaneously. The first objective proposes to explore the phenomenon of multi-affiliations in descriptive terms; the second is to look into the differences-in terms of motivations, family-work-volunteerism reconciliation, life satisfaction, and membership satisfaction-between those who "only" carry out blood donation activity and those who instead participate in multiple associative realities concurrently...
July 25, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28802068/dietary-citrate-and-plasma-ionized-calcium-implications-for-platelet-donors
#19
Stefanie Haynes, Elda Hickson, Jeanne Linden, Patricia St Pierre, Paula Ducharme, Paula Sulmasy, Linda Welch, Yong Zhao, Mindy Greene, Michelle Vauthrin, Robert Weinstein
BACKGROUND: Platelet donors receive 40 mmol or more of IV citrate anion during donation. When plasma ionized calcium ([Ca(2+) ]) falls by ∼20%, half of the donors report symptoms of hypocalcemic toxicity. Citrus juices contain clinically relevant amounts of citrate anion. We asked whether citrus juice can lower [Ca(2+) ] thus potentially contributing to hypocalcemic toxicity. METHOD: Six volunteers were given 20.4 mmol of citrate anion as grapefruit juice or orange juice...
August 12, 2017: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/28801752/biosimilar-g-csf-versus-filgrastim-and-lenograstim-in-healthy-unrelated-volunteer-hematopoietic-stem-cell-donors
#20
Roiya Farhan, Elżbieta Urbanowska, Hanna Zborowska, Małgorzata Król, Maria Król, Tigran Torosian, Iwona Piotrowska, Krzysztof Bogusz, Kamila Skwierawska, Wiesław Wiktor-Jędrzejczak, Emilian Snarski
The World Marrow Donor Organization recommends original granulocyte-colony stimulating factor (G-CSF) for the mobilization of stem cells in healthy unrelated hematopoietic stem cell donors. We report the comparison of a biosimilar G-CSF (Zarzio) with two original G-CSFs (filgrastim and lenograstim) in mobilization in unrelated donors. We included data of 313 consecutive donors who were mobilized during the period from October 2014 to March 2016 at the Medical University of Warsaw. The primary endpoints of this study were the efficiency of CD34+ cell mobilization to the circulation and results of the first apheresis...
August 11, 2017: Annals of Hematology
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