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https://www.readbyqxmd.com/read/28577982/purification-characterization-and-biological-activities-of-a-polysaccharide-from-lepidium-meyenii-leaves
#1
Shufang Li, Limin Hao, Qiaozhen Kang, Yinxin Cui, Hui Jiang, Xin Liu, Jike Lu
The characteristics and biological activities of a novel polysaccharide from Lepidium meyenii leaves (LMLP) were investigated. LMLP was purified using DEAE-52 cellulose chromatography followed by SephadexG-100 chromatography. The average molecule weight of LMLP was 58.43kDa and it was composed of galactose, arabinose, rhamnose, glucose and mannose with a relative molar ratio of 5.51:4.05:1.15:0.77:0.01. Antioxidant activity results showed that LMLP presented an EC50 of 3.72mg/mL in scavenging 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, and it also had reducing power (OD700nm being 0...
May 31, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28560642/identification-and-characterization-of-micrornas-and-their-targets-in-high-altitude-stress-adaptive-plant-maca-lepidium-meyenii-walp
#2
Sujay Paul
MicroRNAs (miRNAs) are endogenous, short (~21-nucleotide), non-coding RNA molecules that play pivotal roles in plant growth, development, and stress response signaling. In this study using recently published draft genome sequence of a high-altitude plant maca (Lepidium meyenii Walp) and applying genome-wide computational-based approaches, a total of 62 potentially conserved miRNAs belonging to 28 families were identified and four (lme-miR160a, lme-miR164c, lme-miR 166a, and lme-miR 319a) of them further validated by RT-PCR...
June 2017: 3 Biotech
https://www.readbyqxmd.com/read/28480222/the-influence-of-ph-of-extracting-water-on-the-composition-of-seaweed-extracts-and-their-beneficial-properties-on-lepidium-sativum
#3
Katarzyna Godlewska, Izabela Michalak, Łukasz Tuhy, Katarzyna Chojnacka
Baltic seaweeds were used to obtain aqueous extracts (E) through changing initial pH of deionised water added to algal biomass (EpH3·H2O, EpH7·H2O, and EpH10·H2O) and through changing pH of the mixture of algae and deionised water (EpH3, EpH7, and EpH10). Algal extracts were characterized in terms of the concentration of polyphenols and micro- and macroelements. The highest concentration of polyphenols was determined in extract EpH3 and the lowest in extract EpH10·H2O. It was found that the obtained extracts had similar concentrations of elements (except EpH3)...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28472063/occurrence-of-urea-based-soluble-epoxide-hydrolase-inhibitors-from-the-plants-in-the-order-brassicales
#4
Seiya Kitamura, Christophe Morisseau, Todd R Harris, Bora Inceoglu, Bruce D Hammock
Recently, dibenzylurea-based potent soluble epoxide hydrolase (sEH) inhibitors were identified in Pentadiplandra brazzeana, a plant in the order Brassicales. In an effort to generalize the concept, we hypothesized that plants that produce benzyl glucosinolates and corresponding isothiocyanates also produce these dibenzylurea derivatives. Our overall aim here was to examine the occurrence of urea derivatives in Brassicales, hoping to find biologically active urea derivatives from plants. First, plants in the order Brassicales were analyzed for the presence of 1, 3-dibenzylurea (compound 1), showing that three additional plants in the order Brassicales produce the urea derivatives...
2017: PloS One
https://www.readbyqxmd.com/read/28448157/protein-changes-in-lepidium-sativum-l-exposed-to-hg-during-soil-phytoremediation
#5
Beata Smolinska, Agnieszka Szczodrowska, Joanna Leszczynska
Some investigations have been carried out in this study to find the best technique of soil reclamation in mercurypolluted soil. In this study, we examined Lepidium sativum L. as a plant useful for Hg phytoextraction. The simultaneous application of compost and thiosulfate was explored as a possible method of enhancing the process of phytoextraction. The results of the investigations of plant protein changes during assisted Hg phytoextraction were also provided. The results of the study show that combined use of compost and thiosulfate significantly increased both the total Hg accumulation and its translocation to aerial plant tissues...
August 3, 2017: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/28429108/phenetic-and-phylogenetic-relationships-among-aceria-spp-acari-eriophyoidea-inhabiting-species-within-the-family-brassicaceae-in-serbia
#6
Zlata Živković, Biljana Vidović, Vida Jojić, Tatjana Cvrković, Radmila Petanović
We examined morphological and genetic differences among Aceria spp. inhabiting six Brassicaceae species in Serbia. Five of them have been already mentioned in the literature as original or alternate hosts of Aceria spp. (Berteroa incana (L.) DC., Capsella bursa-pastoris (L.) Medik., Cardamine hirsuta L., Lepidium draba L. and Sisymbrium orientale L.), whereas Aurinia petraea (Ard.) Schur was registered here for the first time as a host for Aceria sp. Results of morphometric analyses indicated clear differentiation of Aceria spp...
April 20, 2017: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/28386894/photosynthetic-pigments-and-peroxidase-activity-of-lepidium-sativum-l-during-assisted-hg-phytoextraction
#7
Beata Smolinska, Joanna Leszczynska
The study was conducted to evaluate metabolic answer of Lepidium sativum L. on Hg, compost, and citric acid during assisted phytoextraction. The chlorophyll a and b contents, total carotenoids, and activity of peroxidase were determined in plants exposed to Hg and soil amendments. Hg accumulation in plant shoots was also investigated. The pot experiments were provided in soil artificially contaminated by Hg and/or supplemented with compost and citric acid. Hg concentration in plant shoots and soil substrates was determined by cold vapor atomic absorption spectroscopy (CV-AAS) method after acid mineralization...
May 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28350346/bioactive-constituents-obtained-from-the-seeds-of-lepidium-apetalum-willd
#8
Sijian Wang, Pingping Shi, Lu Qu, Jingya Ruan, Shengcai Yang, Haiyang Yu, Yi Zhang, Tao Wang
Three new compounds, apetalumosides C₁ (1), D (2), and 1-thio--d-glucopyranosyl(1→1)-1-thio-α-d-glucopyranoside (3), together with twenty-two known ones (4-25) were obtained from the seeds of Lepidium apetalum Willd. Among the known isolates, 5-8, 10-13, 16-20, and 25 were obtained from the genus for the first time; 4, 14, 15, and 21-24 were isolated from the species for the first time. Meanwhile, the NMR data of 16 was first reported here. Their structures were determined by means of chemical and spectroscopic methods...
March 28, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28343387/hydroxyl-and-methoxyl-derivatives-of-benzylglucosinolate-in-lepidium-densiflorum-with-hydrolysis-to-isothiocyanates-and-non-isothiocyanate-products-substitution-governs-product-type-and-mass-spectral-fragmentation
#9
Eleonora Pagnotta, Niels Agerbirk, Carl E Olsen, Luisa Ugolini, Susanna Cinti, Luca Lazzeri
A system of benzylic glucosinolates was found and characterized in common pepperweed, Lepidium densiflorum Schrad. The major glucosinolate was the novel 4-hydroxy-3,5-dimethoxybenzylglucosinolate (3,5-dimethoxysinalbin), present at high levels in seeds, leaves, and roots. Medium-level glucosinolates were 3,4-dimethoxybenzylglucosinolate and 3,4,5-trimethoxybenzylglucosinolate. Minor glucosinolates included benzylglucosinolate, 3-hydroxy- and 3-methoxybenzylglucosinolate, 4-hydroxybenzylglucosinolate (sinalbin), the novel 4-hydroxy-3-methoxybenzylglucosinolate (3-methoxysinalbin), and indole-type glucosinolates...
April 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28304399/chemical-profiling-analysis-of-maca-using-uhplc-esi-orbitrap-ms-coupled-with-uhplc-esi-qqq-ms-and-the-neuroprotective-study-on-its-active-ingredients
#10
Yanyan Zhou, Peng Li, Adelheid Brantner, Hongjie Wang, Xinbin Shu, Jian Yang, Nan Si, Lingyu Han, Haiyu Zhao, Baolin Bian
Lepidium meyenii (Maca), originated from Peru, has been cultivated widely in China as a popular health care food. However, the chemical and effective studies of Maca were less in-depth, which restricted its application seriously. To ensure the quality of Maca, a feasible and accurate strategy was established. One hundred and sixty compounds including 30 reference standards were identified in 6 fractions of methanol extract of Maca by UHPLC-ESI-Orbitrap MS. Among them, 15 representative active compounds were simultaneously determined in 17 samples by UHPLC-ESI-QqQ MS...
March 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28295471/long-term-feeding-of-hydroalcoholic-extract-powder-of-lepidium-meyenii-maca-enhances-the-steroidogenic-ability-of-leydig-cells-to-alleviate-its-decline-with-ageing-in-male-rats
#11
K Yoshida, Y Ohta, N Kawate, M Takahashi, T Inaba, S Hatoya, H Morii, K Takahashi, M Ito, H Tamada
This study examined whether feeding hydroalcoholic extract of Lepidium meyenii (maca) to 8-week-old (sexually maturing) or 18-week-old (mature) male rats for more than a half year affects serum testosterone concentration and testosterone production by Leydig cells cultured with hCG, 22R-hydroxycholesterol or pregnenolone. Testosterone concentration was determined in the serum samples obtained before and 6, 12, 18 and 24 weeks after the feeding, and it was significantly increased only at the 6 weeks in the group fed with the maca extract to maturing rats when it was compared with controls...
March 10, 2017: Andrologia
https://www.readbyqxmd.com/read/28273569/toxicity-of-combined-mixtures-of-nanoparticles-to-plants
#12
Izabela Jośko, Patryk Oleszczuk, Ewa Skwarek
An increasing production and using of nanoproducts results in releasing and dispersing nanoparticles (NPs) in the environment. Being released into various environment components, NPs may interact with numerous pollutants, including other NPs. This research aimed at assessing toxicity of combined binary mixtures of NPs. The study focused on assessing mixtures of NPs believed to be toxic (nano-ZnO+nano-CuO) and nano-ZnO/nano-CuO with the ones that are insignificantly toxic or non-toxic NPs (nano-TiO2/nano-Cr2O3/nano-Fe2O3)...
February 24, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28262313/high-performance-liquid-chromatography-mass-spectrometry-analysis-of-the-parent-drugs-and-their-metabolites-in-extracts-from-cress-lepidium-sativum-grown-hydroponically-in-water-containing-four-non-steroidal-anti-inflammatory-drugs
#13
Lisa Emhofer, Markus Himmelsbach, Wolfgang Buchberger, Christian W Klampfl
In this paper the metabolism of four non-steroidal anti-inflammatory drugs, (ketoprofen, mefenamic acid, naproxen, and diclofenac) by cress (Lepidium sativum) is described. Cress was cultivated hydroponically in water spiked with the parent drugs at levels ranging from 0.01mgL(-1) to 1mgL(-1). Employing an approach based on the analysis of the plant extracts by HPLC coupled either with quadrupole-time-of-flight mass spectrometry, or Orbitrap MS or triple quadrupole (QqQ) MS allowed the identification of twenty substances (sixteen metabolites and four parent drugs)...
March 31, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28261840/feeding-hydroalcoholic-extract-powder-of-lepidium-meyenii-maca-enhances-testicular-gene-expression-of-3%C3%AE-hydroxysteroid-dehydrogenase-in-rats
#14
Y Ohta, N Kawate, T Inaba, H Morii, K Takahashi, H Tamada
Although feeding diets containing the extract powder of Lepidium meyenii (maca), a plant growing in Peru's Central Andes, increases serum testosterone concentration associated with enhanced ability of testosterone production by Leydig cells in male rats, changes in testicular steroidogenesis-related factors by the maca treatment are not known. This study examined the effects of maca on testicular gene expressions for luteinizing hormone receptor, steroidogenic acute regulatory protein and steroidogenic enzymes...
March 6, 2017: Andrologia
https://www.readbyqxmd.com/read/28242917/optimization-of-the-new-formulation-of-ice-cream-with-native-iranian-seed-gums-lepidium-perfoliatum-and-lepidium-sativum-using-response-surface-methodology-rsm
#15
M Azari-Anpar, N Soltani Tehrani, N Aghajani, M Khomeiri
In this study, effect of Qodume shahri (Lepidium perfoliatum) and cress (Lepidium sativum) on rheological properties of ice cream were investigated. The gums were added to the ice cream formulation and different quality attributes including pH, acidity, melting characteristics, viscosity, overrun, texture analysis and sensory evaluation were determined. Results showed that ice cream formulations containing both the gums had improved overrun, melting rate, first dripping time, viscosity, hardness and adhesiveness...
January 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28238907/carboxymethylation-of-lepidium-sativum-polyuronide-its-characterization-and-evaluation-as-a-nanometric-carrier
#16
Deepika Rani, Munish Ahuja
Carboxymethylation of Lepidium sativum polyuronide was carried out to improve its functionality. Carboxymethyl modification was accomplished by reacting it with monochloroacetic acid under alkaline conditions, which yielded a product with degree of carboxymethyl substitution of 1.75. The results of characterization studies revealed that carboxymethylation of L. sativum polyuronide improves its flow property, changes compression behavior from elastic to plastic, diminishes its viscosity and swelling, and increases its crystallinity...
June 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28231388/structural-characterization-and-antifatigue-effect-in-vivo-of-maca-lepidium-meyenii-walp-polysaccharide
#17
Weimin Tang, Lu Jin, Lianghua Xie, Juqing Huang, Nan Wang, Bingquan Chu, Xulin Dai, Yu Liu, Rui Wang, Ying Zhang
Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo. The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses. The antifatigue effect of MP was evaluated by using a mouse weight-loaded swimming model. MP is an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793...
March 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28223399/centromere-location-in-arabidopsis-is-unaltered-by-extreme-divergence-in-cenh3-protein-sequence
#18
Shamoni Maheshwari, Takayoshi Ishii, C Titus Brown, Andreas Houben, Luca Comai
During cell division, spindle fibers attach to chromosomes at centromeres. The DNA sequence at regional centromeres is fast evolving with no conserved genetic signature for centromere identity. Instead CENH3, a centromere-specific histone H3 variant, is the epigenetic signature that specifies centromere location across both plant and animal kingdoms. Paradoxically, CENH3 is also adaptively evolving. An ongoing question is whether CENH3 evolution is driven by a functional relationship with the underlying DNA sequence...
March 2017: Genome Research
https://www.readbyqxmd.com/read/28212012/-meyeniins-a-c-novel-hexahydroimidazo-1-5-c-thiazole-derivatives-from-the-tubers-of-lepidium-meyenii-complete-structural-elucidation-by-biomimetic-synthesis-and-racemic-crystallization
#19
Min Zhou, Hang-Ying Ma, Zhi-Hua Liu, Guang-Yu Yang, Gang Du, Yan-Qing Ye, Gan-Peng Li, Qiu-Fen Hu
(+)-Meyeniins A-C (1-3), a novel class of sulfur-containing hexahydroimidazo[1,5-c]thiazole derivatives, were isolated from the tubers of Lepidium meyenii (maca) cultivated in Lijiang, Yunnan province, China. Guided by their biosynthetic hypothesis, a stereocontrolled biomimetic synthesis of meyeniins A-C and their individual enantiomers was efficiently accomplished by a combination of a condensation reaction and Edman degradation. The formation of high-quality crystals for X-ray crystallography occurred much more readily from a racemic mixture of (±)-meyeniin A than with the single enantiomer alone in this case...
March 8, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28199876/the-effect-of-sewage-sludge-application-on-soil-properties-and-willow-salix-sp-cultivation
#20
Magdalena Urbaniak, Anna Wyrwicka, Wojciech Tołoczko, Liliana Serwecińska, Marek Zieliński
The aim of the study was to determine the impact of sewage sludge from three wastewater treatment plants of different sizes (small, medium and large) applied in two doses (3 and 9 tons per hectare) on soil properties, determined as the content of organic carbon and humus fractions, bacterial abundance, phytotoxicity and PCDD/PCDF TEQ concentrations. The study also evaluated the impact of this sewage sludge on the biometric and physiological parameters and detoxification reaction of willow (Salix sp.) as a typical crop used for the remediation of soil following sludge application...
May 15, 2017: Science of the Total Environment
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