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https://www.readbyqxmd.com/read/28231388/structural-characterization-and-antifatigue-effect-in-vivo-of-maca-lepidium-meyenii-walp-polysaccharide
#1
Weimin Tang, Lu Jin, Lianghua Xie, Juqing Huang, Nan Wang, Bingquan Chu, Xulin Dai, Yu Liu, Rui Wang, Ying Zhang
Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo. The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses. The antifatigue effect of MP was evaluated by using a mouse weight-loaded swimming model. MP is an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793...
February 23, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28223399/centromere-location-in-arabidopsis-is-unaltered-by-extreme-divergence-in-cenh3-protein-sequence
#2
Shamoni Maheshwari, Takayoshi Ishii, C Titus Brown, Andreas Houben, Luca Comai
During cell division, spindle fibers attach to chromosomes at centromeres. The DNA sequence at regional centromeres is fast evolving with no conserved genetic signature for centromere identity. Instead CENH3, a centromere-specific histone H3 variant, is the epigenetic signature that specifies centromere location across both plant and animal kingdoms. Paradoxically, CENH3 is also adaptively evolving. An ongoing question is whether CENH3 evolution is driven by a functional relationship with the underlying DNA sequence...
February 21, 2017: Genome Research
https://www.readbyqxmd.com/read/28212012/-meyeniins-a-c-novel-hexahydroimidazo-1-5-c-thiazole-derivatives-from-the-tubers-of-lepidium-meyenii-complete-structural-elucidation-by-biomimetic-synthesis-and-racemic-crystallization
#3
Min Zhou, Hang-Ying Ma, Zhi-Hua Liu, Guang-Yu Yang, Gang Du, Yan-Qing Ye, Gan-Peng Li, Qiu-Fen Hu
(+)-Meyeniins A-C (1-3), a novel class of sulfur-containing hexahydroimidazo[1,5-c]thiazole derivatives, were isolated from the tubers of Lepidium meyenii (maca) cultivated in Lijiang, Yunnan province, China. Guided by their biosynthetic hypothesis, a stereocontrolled biomimetic synthesis of meyeniins A-C and their individual enantiomers was efficiently accomplished by a combination of a condensation reaction and Edman degradation. The formation of high-quality crystals for X-ray crystallography occurred much more readily from a racemic mixture of (±)-meyeniin A than with the single enantiomer alone in this case...
February 23, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28199876/the-effect-of-sewage-sludge-application-on-soil-properties-and-willow-salix-sp-cultivation
#4
Magdalena Urbaniak, Anna Wyrwicka, Wojciech Tołoczko, Liliana Serwecińska, Marek Zieliński
The aim of the study was to determine the impact of sewage sludge from three wastewater treatment plants of different sizes (small, medium and large) applied in two doses (3 and 9 tons per hectare) on soil properties, determined as the content of organic carbon and humus fractions, bacterial abundance, phytotoxicity and PCDD/PCDF TEQ concentrations. The study also evaluated the impact of this sewage sludge on the biometric and physiological parameters and detoxification reaction of willow (Salix sp.) as a typical crop used for the remediation of soil following sludge application...
February 12, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28174084/protective-effect-of-polysaccharide-from-maca-lepidium-meyenii-on-hep-g2-cells-and-alcoholic-liver-oxidative-injury-in-mice
#5
Lijun Zhang, Qingsheng Zhao, Liwei Wang, Mingxia Zhao, Bing Zhao
To study the characterization and hepatoprotective activity of polysaccharide from maca (Lepidium meyenii), the main polysaccharide from maca (MP-1) was obtained by DEAE-52 cellulose column. The average molecular weight of MP-1 was 1067.3kDa and the polysaccharide purity was 91.63%. In order to assess the antioxidant activities of MP-1, four kinds of methods were used, including scavenging hydroxyl radical, DPPH, superoxide anion radical, and FRAP, and the results indicated high antioxidant activities. Furthermore, hepatoprotective activity of MP-1 was studied both in vitro and vivo...
February 4, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28157176/the-essential-oil-of-monarda-didyma-l-lamiaceae-exerts-phytotoxic-activity-in-vitro-against-various-weed-seed
#6
Donata Ricci, Francesco Epifano, Daniele Fraternale
The chemical composition of the essential oil of the flowering aerial parts of Monarda didyma L. cultivated in central Italy was analyzed by Gas Chromatography/Mass Spectrometry (GC/MS). The major compounds of the oil were thymol (59.3%), p-cymene (10.3%), terpinolene (9.2%), δ-3-carene (4.4%), myrcene (3.7%), and camphene (3.4%). The essential oil was tested in vitro for its anti-germination activity against Papaver rhoeas L., Taraxacum officinale F. H. Wigg., Avena fatua L., Raphanus sativus L. and Lepidium sativum L...
February 2, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28117590/structural-characterization-of-a-novel-polysaccharide-from-lepidium-meyenii-maca-and-analysis-of-its-regulatory-function-in-macrophage-polarization-in-vitro
#7
Mengmeng Zhang, Wenjia Wu, Yao Ren, Xiaofeng Li, Yuqian Tang, Tian Min, Furao Lai, Hui Wu
In our previous study, three novel polysaccharides, named MC-1, MC-2, and MC-3, were separated from the roots of maca (Lepidium meyenii), which is a food source from the Andes region. The structural information and immunomodulatory activity of MC-1 were then investigated. The structure and activity of MC-2 are still unknown. In this study, structural characterization revealed that MC-2 has an average molecular weight of 9.83 kDa and is composed of arabinose (20.9%), mannose (4.5%), glucose (71.9%), and galactose (2...
February 2, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28115285/luhong-formula-inhibits-myocardial-fibrosis-in-a-paracrine-manner-by-activating-the-gp130-jak2-stat3-pathway-in-cardiomyocytes
#8
Huiyan Qu, Yong Wang, Yingjie Wang, Tao Yang, Zhou Feng, Yang Qu, Hua Zhou
ETHNOPHARMACOLOGICAL RELEVANCE: Luhong formula (LHF)-a traditional Chinese medicine containing Cervus nippon Temminck,Carthamus tinctorius L., Cinnamomum cassia Presl,Codonopisis pilosula( Franch.) Nannf., Astragalus membranaceus ( Fisch.) Bge. var. mongholicus ( Bge.) Hsiao, Lepidium apetalum Willd-is used in the treatment of heart failure. AIM OF THE STUDY: To investigate the antifibrotic efficacy of LHF in a myocardial infarction-induced rat model of heart failure and to determine its mechanism of action...
January 20, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28094157/stabilization-of-tannery-sludge-by-co-treatment-with-aluminum-anodizing-sludge-and-phytotoxicity-of-end-products
#9
E Pantazopoulou, O Zebiliadou, M Mitrakas, A Zouboulis
A global demand for efficient re-utilization of produced solid wastes, which is based on the principles of re-use and recycling, results to a circular economy, where one industry's waste becomes another's raw material and it can be used in a more efficient and sustainable way. In this study, the influence of a by-product addition, such as aluminum anodizing sludge, on tannery waste (air-dried sludge) stabilization was examined. The chemical characterization of tannery waste leachate, using the EN 12457-2 standard leaching test, reveals that tannery waste cannot be accepted even in landfills for hazardous wastes, according to the EU Decision 2003/33/EC...
January 13, 2017: Waste Management
https://www.readbyqxmd.com/read/28078724/application-of-multi-block-analysis-and-mixture-design-with-process-variable-for-development-of-chocolate-cake-containing-yacon-smallanthus-sonchifolius-and-maca-lepidium-meyenii
#10
Marcela Marta Lazaretti Tormena, Luana Tabalipa de Medeiros, Patrícia Casarin de Lima, Gabriela Possebon, Renata Hernandez Barros Fuchs, Evandro Bona
BACKGROUND: In this study, a chocolate cake formulation was developed with partial substitution of wheat flour by yacon and maca flour. A simplex-centroid design was applied to determine the proportions of the three flours and the amount of water was included as a process variable at three distinct levels. According to the overall acceptability of the cakes, the tasters were separated into two groups using the k-means. RESULTS: After segmentation, regression models were constructed for overall acceptability of each group, it is obtained R(2)ajusted values of 92...
January 12, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28007001/antioxidant-and-neuroprotector-effect-of-lepidium-meyenii-maca-methanol-leaf-extract-against-6-hydroxy-dopamine-6-ohda-induced-toxicity-in-pc12-cells
#11
Ángel Rodríguez-Huamán, Sandra Casimiro-Gonzales, Jorge Antonio Chávez-Pérez, Carla Gonzales-Arimborgo, Richard Cisneros-Fernández, Luis Ángel Aguilar-Mendoza, Gustavo F Gonzales
Reactive oxygen species (ROS) are normally produced during cell metabolism, there is strong evidence to suggest that ROS produced in excess impair the cell and may be etiologically related to various neurodegenerative diseases. This study was undertaken to examine the effects of Lepidium meyenii (MACA) methanol leaf extract on neurotoxicity in PC12 cell exposed to 6-hydroxydopamine (6-OHDA). Fresh samples of "maca" leaves were processed in order to obtain foliar extracts and to evaluate the neurobiological activity on PC12 cells, subjected to the cytotoxic effect of 6-OHDA through the determination of the capacity antioxidant, cell viability and cytotoxicity assays on PC12 cells...
January 8, 2017: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/27912738/hepatoprotective-activity-of-lepidium-sativum-seeds-against-d-galactosamine-lipopolysaccharide-induced-hepatotoxicity-in-animal-model
#12
Mohammad Raish, Ajaz Ahmad, Khalid M Alkharfy, Syed Rizwan Ahamad, Kazi Mohsin, Fahad I Al-Jenoobi, Abdullah M Al-Mohizea, Mushtaq Ahmad Ansari
BACKGROUND: Fulminant hepatic failure (FHF) is clinical syndrome with very poor prognosis and high mortality there is urgent need for the development of safe and non-toxic hepatoprotective agents for the adequate management of hepatitis. Hepatoprotective effect of the Lepidium sativum ethanolic extract (LSEE) was assessed by D-galactosamine-induced/lipopolysaccharide (400 mg/kg and 30 μg/kg) liver damage model in rats. METHODS: Hepatoprotective activity of LSEE (150 and 300 mg/kg) and silymarin on D-GalN/LPS induced FHF in rat was assessed using several liver function enzyme parameters...
December 3, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27871147/shared-and-host-specific-microbiome-diversity-and-functioning-of-grapevine-and-accompanying-weed-plants
#13
Abdul Samad, Friederike Trognitz, Stéphane Compant, Livio Antonielli, Angela Sessitsch
Weeds and crop plants select their microbiota from the same pool of soil microorganisms, however, the ecology of weed microbiomes is poorly understood. We analyzed the microbiomes associated with roots and rhizospheres of grapevine and four weed species (Lamium amplexicaule L., Veronica arvensis L., Lepidium draba L. and Stellaria media L.) growing in proximity in the same vineyard using 16S rRNA gene sequencing. We also isolated and characterized 500 rhizobacteria and root endophytes from L. draba and grapevine...
November 21, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27795982/profile-of-trace-elements-in-selected-medicinal-plants-used-for-the-treatment-of-diabetes-in-eritrea
#14
Mussie Sium, Patrick Kareru, Joseph Keriko, Berhane Girmay, Ghebrehiwet Medhanie, Semere Debretsion
This study was designed to investigate the profile of certain trace elements having therapeutic properties related to diabetes mellitus. The investigated plants were Aloe camperi, Meriandra dianthera, Lepidium sativum, Brassica nigra, and Nigella sativa. These plants are traditionally used in the management of diabetes in Eritrea. The elemental analysis was conducted using inductively coupled plasma optical emission spectrometry (ICP-OES) and flame atomic absorption spectroscopy (FAAS) techniques. The accuracy of the methods was verified using in-house reference materials (CRMs) and no significant differences were observed between the measured and certified values...
2016: TheScientificWorldJournal
https://www.readbyqxmd.com/read/27795282/high-quality-draft-genome-sequence-of-an-endophytic-pseudomonas-viridiflava-strain-with-herbicidal-properties-against-its-host-the-weed-lepidium-draba-l
#15
Abdul Samad, Friederike Trognitz, Livio Antonielli, Stéphane Compant, Angela Sessitsch
Here, we report the draft genome sequence of Pseudomonas viridiflava strain CDRTc14 a pectinolytic bacterium showing herbicidal activity, isolated from the root of Lepidium draba L. growing as a weed in an Austrian vineyard. The availability of this genome sequence allows us to investigate the genetic basis of plant-microbe interactions.
October 20, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27719949/physicochemical-properties-of-maca-starch
#16
Ling Zhang, Guantian Li, Sunan Wang, Weirong Yao, Fan Zhu
Maca (Lepidium meyenii Walpers) is gaining research attention due to its unique bioactive properties. Starch is a major component of maca roots, thus representing a novel starch source. In this study, the properties of three maca starches (yellow, purple and black) were compared with commercially maize, cassava, and potato starches. The starch granule sizes ranged from 9.0 to 9.6μm, and the granules were irregularly oval. All the maca starches presented B-type X-ray diffraction patterns, with the relative degree of crystallinity ranging from 22...
March 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27718531/living-in-the-city-urban-environments-shape-the-evolution-of-a-native-annual-plant
#17
Mohamed Yakub, Peter Tiffin
Urban environments are warmer, have higher levels of atmospheric CO2 and have altered patterns of disturbance and precipitation than nearby rural areas. These differences can be important for plant growth and are likely to create distinct selective environments. We planted a common garden experiment with seeds collected from natural populations of the native annual plant Lepidium virginicum, growing in five urban and nearby rural areas in the northern United States to determine whether and how urban populations differ from those from surrounding rural areas...
October 8, 2016: Global Change Biology
https://www.readbyqxmd.com/read/27708692/in-silico-profiling-for-secondary-metabolites-from-lepidium-meyenii-maca-by-the-pharmacophore-and-ligand-shape-based-joint-approach
#18
Fan Yi, Xiao-Lei Tan, Xin Yan, Hai-Bo Liu
BACKGROUND: Lepidium meyenii Walpers (maca) is an herb known as a traditional nutritional supplement and widely used in Peru, North America, and Europe to enhance human fertility and treat osteoporosis. The secondary metabolites of maca, namely, maca alkaloids, macaenes, and macamides, are bioactive compounds, but their targets are undefined. METHODS: The pharmacophore-based PharmaDB targets database screening joint the ligand shape similarity-based WEGA validation approach is proposed to predict the targets of these unique constituents and was performed using Discovery Studio 4...
2016: Chinese Medicine
https://www.readbyqxmd.com/read/27704185/emerging-concern-from-short-term-textile-leaching-a-preliminary-ecotoxicological-survey
#19
G Lofrano, G Libralato, M Carotenuto, M Guida, M Inglese, A Siciliano, S Meriç
Textile dyes and their residues gained growing attention worldwide. Textile industry is a strong water consumer potentially releasing xenobiotics from washing and rinsing procedures during finishing processes. On a decentralised basis, also final consumers generate textile waste streams. Thus, a procedure simulating home washing with tap water screened cotton textiles leachates (n = 28) considering physico-chemical (COD, BOD5, and UV absorbance) and ecotoxicological data (Daphnia magna, Pseudokirchneriella subcapitata and Lepidium sativum)...
November 2016: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/27681650/effect-of-lepidium-meyenii-maca-on-testicular-function-of-mice-with-chemically-and-physically-induced-subfertility
#20
M Valdivia Cuya, K Yarasca De La Vega, G Lévano Sánchez, J Vásquez Cavero, H Temoche García, L Torres Torres, V Cruz Ornetta
The aim of this study was to evaluate the effect of Lepidium meyenii (maca) in chemically and physically subfertile mice. After 35 days, the following groups of mice were evaluated: control, sham, chemical subfertility, chemical subfertility-maca-supplemented, physical subfertility, physical subfertility-maca-supplemented and maca-supplemented only. Motility (32.36% ± 5.34%) and sperm count (44.4 ± 5.37 × 10(6) /ml) in the chemically and physically subfertile mice (11.81% ± 4.06%, 17.34 ± 13...
October 2016: Andrologia
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