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https://www.readbyqxmd.com/read/29781619/optically-detected-magnetic-resonance-of-the-chlorophyll-triplet-state-in-the-water-soluble-chlorophyll-protein-from-lepidium-virginicum-evidence-for-excitonic-interaction-among-the-four-pigments
#1
Alessandro Agostini, Daniel M Palm, Harald Paulsen, Donatella Carbonera
Optically detected magnetic resonance (ODMR) of triplet states populated by photoexcitation in the Water-Soluble Chlorophyll Protein (WSCP) from Lepidium virginicum has been performed, using both absorption and fluorescence detection. Well resolved Triplet minus Singlet (T-S) spectra have been obtained, which have been interpreted in terms of electronic interactions among the four chlorophylls (Chls) forming two dimers in WSCP tetramer. Localization of the triplet state on a single Chl leads to a redistribution of the oscillator strenght in the remaining three Chls of the complex...
May 21, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29779954/construction-of-fluorescent-analogs-to-follow-the-uptake-and-distribution-of-cobalamin-vitamin-b-12-in-bacteria-worms-and-plants
#2
Andrew D Lawrence, Emi Nemoto-Smith, Evelyne Deery, Joseph A Baker, Susanne Schroeder, David G Brown, Jennifer M A Tullet, Mark J Howard, Ian R Brown, Alison G Smith, Helena I Boshoff, Clifton E Barry, Martin J Warren
Vitamin B12 is made by only certain prokaryotes yet is required by a number of eukaryotes such as mammals, fish, birds, worms, and Protista, including algae. There is still much to learn about how this nutrient is trafficked across the domains of life. Herein, we describe ways to make a number of different corrin analogs with fluorescent groups attached to the main tetrapyrrole-derived ring. A further range of analogs were also constructed by attaching similar fluorescent groups to the ribose ring of cobalamin, thereby generating a range of complete and incomplete corrinoids to follow uptake in bacteria, worms, and plants...
April 28, 2018: Cell Chemical Biology
https://www.readbyqxmd.com/read/29744941/carvacrol-and-human-health-a-comprehensive-review
#3
REVIEW
Mehdi Sharifi-Rad, Elena Maria Varoni, Marcello Iriti, Miquel Martorell, William N Setzer, María Del Mar Contreras, Bahare Salehi, Azam Soltani-Nejad, Sadegh Rajabi, Mercedeh Tajbakhsh, Javad Sharifi-Rad
Carvacrol (CV) is a phenolic monoterpenoid found in essential oils of oregano (Origanum vulgare), thyme (Thymus vulgaris), pepperwort (Lepidium flavum), wild bergamot (Citrus aurantium bergamia), and other plants. Carvacrol possesses a wide range of bioactivities putatively useful for clinical applications such antimicrobial, antioxidant, and anticancer activities. Carvacrol antimicrobial activity is higher than that of other volatile compounds present in essential oils due to the presence of the free hydroxyl group, hydrophobicity, and the phenol moiety...
May 9, 2018: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29735098/determination-of-isothiocyanate-protein-conjugates-in-milk-and-curd-after-adding-garden-cress-lepidium-sativum-l
#4
Carla Kühn, Tobias von Oesen, Franziska S Hanschen, Sascha Rohn
Isothiocyanates (ITC) play an important role in health promotion and cancer prevention due to their anti-bacterial, anti-inflammatory, and anti-cancerogenic properties. However, ITC are highly reactive so that a reaction with further food components is very likely. For example, a reaction of ITC with nucleophilic amino acid side chains of proteins such as cysteine and lysine can occur, reducing the bioavailability of indispensable amino acids and protein functions may be altered. Therefore, it is of great interest to investigate the fate of ITC in the food matrix...
June 2018: Food Research International
https://www.readbyqxmd.com/read/29681858/aqueous-extract-of-black-maca-prevents-metabolism-disorder-via-regulating-the-glycolysis-gluconeogenesis-tca-cycle-and-ppar%C3%AE-signaling-activation-in-golden-hamsters-fed-a-high-fat-high-fructose-diet
#5
Wenting Wan, Hongxiang Li, Jiamei Xiang, Fan Yi, Lijia Xu, Baoping Jiang, Peigen Xiao
Maca ( Lepidium meyenii Walpers) has been used as a dietary supplement and ethnomedicine for centuries. Recently, maca has become a high profile functional food worldwide because of its multiple biological activities. This study is the first explorative research to investigate the prevention and amelioration capacity of the aqueous extract of black maca (AEM) on high-fat, high-fructose diet (HFD)-induced metabolism disorder in golden hamsters and to identify the potential mechanisms involved in these effects...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29627468/cross-linking-effect-of-polyphenolic-extracts-of-lepidium-sativum-seedcake-on-physicochemical-properties-of-chitosan-films
#6
Deepak Kadam, S S Lele
Lepidium sativum seedcake phenolic extract (LSE), as compared to tannic acid (TA), was evaluated for its effect on film forming ability of chitosan. The films were investigated for their structural, mechanical, optical, thermal and in vitro antioxidant activity release profile. At 5% (v/v of film-forming solution), LSE led to improved 32.2% of tensile strength and 109% elongation, compared to the effect of TA in chitosan films. Moisture content, WVP, and crystallinity decreased with the increasing LSE concentration...
April 5, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29568754/monoamine-oxidase-a-inhibition-and-associated-antioxidant-activity-in-plant-extracts-with-potential-antidepressant-actions
#7
Tomás Herraiz, Hugo Guillén
Monoamine oxidase (MAO) catalyzes the oxidative deamination of amines and neurotransmitters and is involved in mood disorders, depression, oxidative stress, and adverse pharmacological reactions. This work studies the inhibition of human MAO-A by Hypericum perforatum , Peganum harmala, and Lepidium meyenii, which are reported to improve and affect mood and mental conditions. Subsequently, the antioxidant activity associated with the inhibition of MAO is determined in plant extracts for the first time. H. perforatum inhibited human MAO-A, and extracts from flowers gave the highest inhibition (IC50 of 63...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29544368/structure-analysis-and-anti-fatigue-activity-of-a-polysaccharide-from-lepidium-meyenii-walp
#8
Yun Tang, Zhen-Yuan Zhu, Li-Chao Pan, Huiqing Sun, Qiao-Ying Song, Yongmin Zhang
A polysaccharide was obtained from Lepidium meyenii Walp by hot water extraction and purification by Millipore (100 kD) and Sephadex G-200. The content of polysaccharide was examined to be 89.9% with phenol-sulfuric acid method. Its average molecular weight was estimated to be 2.213 × 106  Da by High Performance Gel Permeation Chromatography (HPGPC). Monosaccharide analysis showed that the polysaccharide was composed of arabinose, mannose, glucose and galactose with the molar ratio of 2.134: 1: 2.78: 2...
March 16, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29524221/postharvest-ultrasound-assisted-freeze-thaw-pretreatment-improves-the-drying-efficiency-physicochemical-properties-and-macamide-biosynthesis-of-maca-lepidium-meyenii
#9
Jin-Jin Chen, Peng-Fei Gong, Yi-Lan Liu, Bo-Yan Liu, Dawn Eggert, Yuan-Heng Guo, Ming-Xia Zhao, Qing-Sheng Zhao, Bing Zhao
A novel technique of ultrasound-assisted freeze-thaw pretreatment (UFP) was developed to improve the drying efficiency of maca and bioactive amide synthesis in maca. The optimal UFP conditions are ultrasonic processing 90 min at 30 °C with 6 freeze-thaw cycles. Samples with freeze-thaw pretreatment (FP), ultrasound pretreatment (UP), and UFP were prepared for further comparative study. A no pretreatment (NP) sample was included as a control. The results showed that UFP improved the drying efficiency of maca slices, showing the highest effective moisture diffusivity (1...
April 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29506633/the-evaluation-of-antioxidant-and-anticancer-effects-of-lepidium-sativum-subsp-spinescens-l-methanol-extract-on-cancer-cells
#10
Sahabettin Selek, Ismail Koyuncu, Hifa Gulru Caglar, Ibrahim Bektas, Mustafa Abdullah Yilmaz, Ataman Gonel, Enes Akyuz
In recent years, there is an increased research interest for plants which are natural sources of antioxidants. Lepidium sativum Subsp spinescens L., commonly found in South West Asia, is a plant known as a healthy nutritional source containing bio-molecules that carry anti-hypertensive, hypoglycemic, anti-asthmatic, antispasmodic, hepato-protective, chemoprotective, anti-inflammatory and anti-oxidant effects. In this study, we aimed to investigate the antioxidant content and activity of Lepidium sativum Subsp spinescens L...
February 28, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29487958/use-of-chemical-indicators-and-bioassays-in-bottom-sediment-ecological-risk-assessment
#11
Marek Tarnawski, Agnieszka Baran
This study is was designed to assess the ecological risk associated with chemical pollution caused by heavy metals and PAHs on the basis of their ecotoxicological properties in sediments collected from the Rzeszów dam reservoir (Poland). The sediment samples were collected from three sampling stations: S1-inlet, backwater station, S2-middle of reservoir, S3 outlet, near the dam. The sediments' toxicity was evaluated using a battery of bioassays (Phytotoxkit, Phytotestkit, Ostracodtoxkit F, and Microtox). The highest content of metals (120...
April 2018: Archives of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/29487458/lc-esi-q-tof-ms-ms-profiling-and-antioxidant-activity-of-phenolics-from-l-sativum-seedcake
#12
Deepak Kadam, Shanooba Palamthodi, S S Lele
Lepidium sativum is widely used as a culinary and medicinal herb and is claimed to cure many diseases. In this study, an attempt was made to investigate the biochemical composition and functional properties of L. sativum ethanolic extract. The extract contained a total phenolic content of 11.03 ± 0.75 (mg GAE/g dw plant material) and a flavonoid content of 4.79 ± 0.24 (mg QE/100 g dw plant material). Further, the extract was characterized by LC-ESI-Q-TOF-MS/MS profiling and the results showed that the ethanolic fraction contains many important phenolics such as Kaempferol, Coumaroylquinic acid, p -Coumaroyl glycolic acid, Caffeic acid...
March 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29474988/changes-of-total-and-freely-dissolved-polycyclic-aromatic-hydrocarbons-and-toxicity-of-biochars-treated-with-various-aging-processes
#13
Patryk Oleszczuk, Michał Kołtowski
The aim of this study was to determine the effect of biochar aging on the content of polycyclic aromatic hydrocarbons (PAHs) (the total content - Ctot , and the freely dissolved - Cfree ) in biochar and its ecotoxicity. Two biochars (BCS and BCM) with varying properties were aged for 420 days at different temperatures (-20 °C, 4 °C, 20 °C, 70 °C), at a variable temperature (-20/20 °C), in the presence of nutrients, and in the presence of inoculum and nutrients. After the aging process, Ctot and Cfree PAHs were determined in samples obtained and an ecotoxicological analysis was performed, which involved tests with bacteria (Vibrio fischeri), invertebrates (Folsomia candida) and plants (Lepidium sativum)...
June 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29448811/production-of-a-new-mucilage-compound-in-lepidium-sativum-callus-by-optimizing-in-vitro-growth-conditions
#14
Pooran Golkar, Fatemeh Hadian, Mehrana Koohi Dehkordi
The mucilage in Lepidium sativum L. is considered a biologically active compound with diverse medicinal properties. Different explants (hypocotyls and leaf) were transferred to Murashige and Skoog (MS) medium supplemented with twelve different plant growth regulator combinations under two different incubations (light and dark). The best mucilage production from callus (36.76% g g-1 dry weight) was obtained in the MS medium supplemented with 1 mg L-1 of 2, 4-D and 2 mg L-1 of BAP under the light condition...
February 15, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29427324/uptake-and-metabolism-of-the-antidepressants-sertraline-clomipramine-and-trazodone-in-a-garden-cress-lepidium-sativum-model
#15
Bernd Reichl, Markus Himmelsbach, Lisa Emhofer, Christian W Klampfl, Wolfgang Buchberger
Environmental contamination with pharmaceuticals has received growing attention in recent years. Several studies describe the presence of traces of drugs in water bodies and soils and their impacts on nontarget organisms including plants. Due to these facts investigations of the uptake and metabolism of pharmaceuticals in organisms is an emerging research area. The present study demonstrates the analysis of three selected antidepressants (sertraline, clomipramine, and trazodone) as well as metabolites and transformation products in a cress model (Lepidium sativum)...
February 10, 2018: Electrophoresis
https://www.readbyqxmd.com/read/29426208/tannery-mixed-liquors-from-an-ecotoxicological-and-mycological-point-of-view-risks-vs-potential-biodegradation-application
#16
Valeria Tigini, Federico Bevione, Valeria Prigione, Anna Poli, Lucrezia Ranieri, Francesco Spennati, Giulio Munz, Giovanna Cristina Varese
Fungi are known to be present in the activated sludge of wastewater treatment plants (WWTP). Their study should be at the base of an overall vision of the plant effectiveness and of effluents sanitary impact. Moreover, it could be fundamental for the implementation of successful bioaugmentation strategies aimed at the removal of recalcitrant or toxic compounds. This is one of the first studies on the cultivable autochthonous mycoflora present in the mixed liquors of two WWTP treating either vegetable or chromium tannery effluents...
June 15, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29359412/influences-of-dietary-supplementation-with-lepidium-meyenii-maca-on-stallion-sperm-production-and-on-preservation-of-sperm-quality-during-storage-at-5-%C3%A2-c
#17
C Del Prete, S Tafuri, F Ciani, M P Pasolini, F Ciotola, S Albarella, D Carotenuto, V Peretti, N Cocchia
Stallion semen is damaged by oxidative stress during cooling and transport. Semen processing and extenders have been tested to improve the fertilizing capacity of semen and to preserve semen during transport. Dietary supplementation with natural antioxidants has been proposed to prevent oxidative damages. In this study, for the first time, the effect of dietary supplementation with Lepidium meyenii (Maca) on the characteristics of fresh and chilled stallion semen was evaluated. Maca is a traditional Andean crop used as a nutraceutical for the fertility-enhancing properties that are linked with antioxidant activity...
March 2018: Andrology
https://www.readbyqxmd.com/read/29353155/isolation-purification-structural-characterization-and-immunostimulatory-activity-of-water-soluble-polysaccharides-from-lepidium-meyenii
#18
Zhengqi Zha, Su-Yan Wang, Weihua Chu, Yang Lv, Hongjin Kan, Qiuli Chen, Lili Zhong, Long Yue, Jinna Xiao, Ying Wang, Hongping Yin
A water-soluble polysaccharide LMP-1 was isolated and purified by ion-exchange chromatography from maca (Lepidium meyenii Walp.). LMP-1 has a molecular weight of 1.01 × 104  Da, and is composed of glucose and arabinose with a molar ratio of 7.03:1.08. Methylation and the 1D and 2D NMR spectroscopy of LMP-1 revealed that it is mainly composed of →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3)-α-D-Glcp-(1→, and β-D-Araf-(1→, with branching at O-6 of →4,6)-α-D-Glcp-(1 → . LMP-1 showed up-regulation of Toll-like receptor 4 (TLR4) and Toll-like receptor 2 (TLR2)...
March 2018: Phytochemistry
https://www.readbyqxmd.com/read/29341924/maca-polysaccharides-a-review-of-compositions-isolation-therapeutics-and-prospects
#19
REVIEW
Yujuan Li, Fangxue Xu, Mengmeng Zheng, Xiaozhi Xi, Xiaowei Cui, Chunchao Han
Maca polysaccharides, some of the major bioactive substances in Lepidium meyenii (Walp.) (Maca), have various biological properties, including anti-oxidant, anti-fatigue, anti-tumor, and immunomodulatory effects, as well as hepatoprotective activity and regulation function. Although many therapeutics depend on multiple structures of maca polysaccharides in addition to providing sufficient foundations for maca polysaccharide products in industrial applications, the relationships between the pharmacological effects and structures have not been established...
January 13, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29313251/unintentional-and-sequential-lead-exposure-from-a-ceramic-mug-and-maca-lepidium-meyenii
#20
Kelly Johnson-Arbor, Kathy Vo, Flavia Wong, Ryszard Gajek
Although the incidence of lead poisoning has decreased in the USA over the last 30 years, human exposures to lead-containing products are still reported. We present a case of unintentional lead exposure from a store-bought ceramic mug and a nutritional supplement. A 32-year-old female was found to have a whole blood lead concentration of 44 μg/dL. Evaluation of her home, occupation, and hobbies initially did not identify a source of lead exposure. Further investigation revealed that the likely etiology of the exposure was lead leaching from a ceramic mug used by the patient to drink hot lemon water while she was pregnant...
January 8, 2018: Journal of Medical Toxicology: Official Journal of the American College of Medical Toxicology
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