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J Manuel Bello-López, Cristina Castañeda-García, Celerino Muñoz-Estrada, Antonio José Machorro-Peréz
INTRODUCTION: Quality control for the detection of infectious markers in blood banks is a necessary activity to ensure the accuracy of donor screening results. Considering that in Mexico blood safety is one of the goals of the National Action Programs, it is essential to evaluate banks through an External Quality Control Program. OBJECTIVE: To analyze one of the evaluations that showed the greatest participation (2014-2/lot46) of banks in the Mexican Republic in the detection of transfusion-transmitted diseases...
January 11, 2018: Transfusion and Apheresis Science
Zhengang Shan, Jieting Huang, Qiao Liao, Ke Huang, Min Wang, Ru Xu, Xi Tang, Weiyun Zhang, Kenrad Nelson, Yongshui Fu, Chengyao Li, Xia Rong
BACKGROUND: Natural killer (NK) cells are critical components in innate immune response to viral infection. Killer cell immunoglobulin-like receptors (KIRs) are involved in regulating the balance of activation or inhibitory function of NK cells. However, the association of KIRs with the spontaneous clearance of hepatitis C virus (HCV) remains unclear in the Chinese population. STUDY DESIGN AND METHODS: A total of 407 HCV-seropositive voluntary blood donors were recruited, including 203 with spontaneous viral clearance and 204 with chronic infection...
February 15, 2018: Transfusion
Young-Chan Kwon, Keith Meyer, Guangyong Peng, Soumya Chatterjee, Daniel F Hoft, Ranjit Ray
A comprehensive strategy to control HCV infection needs a vaccine. Our phase I study with recombinant HCV E1/E2 EnvGPs as a candidate vaccine did not induce a strong immune response in volunteers. We analyzed the interactions of HCV EnvGPs with human monocyte derived macrophages as antigen presenting cells. HCV E2 induced immune regulatory cytokine IL-10 and sCD163 protein expression in macrophages from 7 of 9 blood donors tested. Further, HCV E2 enhanced Stat3 and suppressed Stat1 activation, reflecting macrophage polarization towards M2 phenotype...
February 14, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Masoud Behzadifar, Hassan Abolghasem Gorji, Nicola Luigi Bragazzi
One of the major infections transmitted through the bloodstream is hepatitis C virus (HCV) infection. Patients with thalassemia requiring frequent blood transfusions are at risk for HCV. Therefore, this study was conducted to determine the incidence of HCV in thalassemia patients in Iran. The following databases were searched: ISI/Web of Science, Embase, Pubmed/MEDLINE via Ovid, Scopus, as well as Iranian databases (Magiran, Irandoc and SID) from January 2000 to July 2017. The quality of the studies was evaluated using the 22-item STROBE checklist...
February 6, 2018: Archives of Virology
Elissa K Butler, Jeffrey McCullough
BACKGROUND: Blood safety and transfusion-transmitted infections (TTIs) are a major concern in low-resource areas. Laboratory screening of donors, a key contributor to blood safety, is usually done by enzyme-linked immunosorbent assay (ELISA) methods, which use expensive reagents and necessitate complex instruments and sophisticated laboratory staff. Rapid diagnostic tests (RDTs) are less expensive and easier to perform but have less sensitivity. Pathogen reduction technology (PRT) reduces transfusion transmission of malaria and may be effective in decreasing other TTIs...
February 5, 2018: Transfusion
Salman Khazaei, Manoochehr Karami, Erfan Ayubi, Abolfazl Mohammadbeigi, Azim Hasanbeigi, Kamyar Mansori, Mohammad Reza Mir-Shekar
Background: Hepatitis B virus (HBV) and hepatitis C virus (HCV) infections are the main causes of severe liver failure worldwide. This study was conducted to evaluate the incidence rate and trend of these infections over six successive years from 2008 to 2013 in Ilam Province, western Iran. Methods: We studied the registered data of HBV and HCV based on the National Notifiable Diseases Surveillance System in Ilam Province from 2008 to 2013. Incidence rate per 100,000 populations was estimated for HBV and HCV infections through location, years and age groups of patients...
2018: Caspian Journal of Internal Medicine
Daniele Rocha, Elisabete Andrade, Daniela T Godoy, Marcela Fontana-Maurell, Elaine Costa, Marisa Ribeiro, Antônio G P Ferreira, Rodrigo Brindeiro, Amilcar Tanuri, Patrícia Alvarez
BACKGROUND: The history of the development and implementation of the Brazilian nucleic acid testing (NAT) platform to detect and discriminate among human immunodeficiency virus (HIV), hepatitis C virus (HCV), and hepatitis B virus (HBV) infections in blood donors is described here. The results for the sensitivity, reproducibility, and NAT yield of the platform since program implementation are provided. STUDY DESIGN AND METHODS: The Brazilian NAT HIV, HCV, and HBV kit was developed and evaluated with regard to analytical sensitivity, specificity, intralot and interlot reproducibility, interfering substances, and genotype and diagnostic sensitivity...
January 30, 2018: Transfusion
Laura Santangelo, Veronica Bordoni, Claudia Montaldo, Eleonora Cimini, Alessandra Zingoni, Cecilia Battistelli, Gianpiero D'Offizi, Maria Rosaria Capobianchi, Angela Santoni, Marco Tripodi, Chiara Agrati
BACKGROUND AND AIMS: Chronic Hepatitis C virus (HCV) infection is known to cause major alterations in the cross-talk between hepatic and immune cells thus contributing to the liver disease pathogenesis. Extracellular vesicles have been proved to act as major players in cell-cell communication, and their cargo changes in relation to pathophysiological states. The aim of this study was to evaluate the effects of chronic HCV infection and Direct-Acting Antivirals (DAA) on exosome-delivered microRNAs and on their ability to modulate the innate immune response...
January 22, 2018: Liver International: Official Journal of the International Association for the Study of the Liver
Ting Xu, Zhong-Mei Yi, Jun-Mei Luo, He-Lian Yu, Ya-Han Fan, Hua Lu, Shu-Ming Zhao, Tian-Lun Jiang
Background: The high prevalence of transfusion-transmitted infections (TTIs) is causing serious harm to human health worldwide. The aim of this research was to assess the prevalence and influencing factors of TTIs in Southwest China. Methods: A retrospective study of blood donor records from January 2008 to December 2015 was conducted. All samples were screened for HBV, HCV, HIV and syphilis. The donor's data was recorded and analyzed statistically using SPSS software...
January 17, 2018: Journal of Public Health
Noor Rehman, M Bilal Orakzai, Azam Hayat, Sadiq Azam, Bashir Ahmad, Ibrar Khan, Zermina Zeb
The current study was designed in order to elucidate the most sensitive method for daily practice as well as to evaluate the risk factors for HCV infection associated with blood transfusion in District Peshawar. A total of 1400 healthy volunteer blood donors were tested for Anti-HCV. A questionnaire was used to evaluate the risk factors. Initial testing of all blood samples was done by Immuno Chromatographic Technique (ICT) and confirmed by micro particle enzyme immunoassay (MEIA) and Enzyme Linked Immunosorbent Assay (ELISA)...
January 2018: Pakistan Journal of Pharmaceutical Sciences
Hana Safic Stanic, Ivana Babic, Margareta Maslovic, Vesna Dogic, Jasna Bingulac-Popovic, Manuela Miletic, Nina Jurakovic-Loncar, Tomislav Vuk, Maja Strauss-Patko, Irena Jukic
Background: Croatia implemented individual donation (ID)-NAT testing of blood donors in 2013 for three viruses HBV, HCV, and HIV-1 as a mandatory test for all blood donors. This study assessed the impact of NAT screening 3 years after its implementation. Methods: A total of 545,463 donations were collected and screened for HBV, HCV, and HIV-1 using the Procleix Ultrio Plus Assay. All initially reactive (IR) NAT samples were retested in triplicate and, if repeatedly reactive (RR), NAT discriminatory assay (dNAT) was performed...
November 2017: Transfusion Medicine and Hemotherapy
Douglas F Taylor, Ryan S Cho, Jason F Okulicz, Bryant J Webber, John G Gancayco
Hepatitis B virus (HBV) and hepatitis C virus (HCV) can cause significant morbidity in military service members. Prevalences of HBV and HCV infections among military recruits accessioning into the U.S. Air Force have not previously been described. The Joint Base San Antonio-Lackland Blood Donor Center was queried for the results of HBV and HCV screening tests among all basic military trainees who donated blood between 25 November 2013 and 16 April 2016. Other active and reserve component members were excluded...
December 2017: MSMR
Majid Mohsenizadeh, Hamid Reza Mollaei, Motahareh Ghaziizadeh
Background: Infections transmitted through blood transfusions are the most important issue associated with blood donation. We aimed to provide an assessment of the prevalence of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV) among blood donors in Kerman province of Iran. Methods: Between 2014-2016, 99,187 samples were examined in a retrospective study in five blood transfusion centers in Kerman province. Serologic screening for HBsAg, anti-HCV and anti-HIV1/2 was conducted for all samples and positive cases were confirmed...
December 29, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
Anuradha Raj, Garima Mittal, Harsh Bahadur
PURPOSE: The purpose of this study was to evaluate the serological profile of the eye donors and to study the influence of various factors on serological test results. METHODS: A cross-sectional, observational study was conducted, and data of 509 donors were reviewed from the records of eye bank from December 2012 to June 2017. Various details of donors analyzed included the age, sex of the donor, cause of death, source of tissue, time since blood collection after death, macroscopic appearance of blood sample, and details of discarded tissues...
January 2018: Indian Journal of Ophthalmology
Susan A Galel, Toby L Simon, Phillip C Williamson, James P AuBuchon, Dan A Waxman, Yasuko Erickson, Rasa Bertuzis, John R Duncan, Khushbeer Malhotra, Jeffrey Vaks, Nancy Huynh, Lisa Lee Pate
BACKGROUND: Use of nucleic acid testing (NAT) in donor infectious disease screening improves transfusion safety. Advances in NAT technology include improvements in assay sensitivity and system automation, and real-time viral target discrimination in multiplex assays. This article describes the sensitivity and specificity of cobas MPX, a multiplex assay for detection of human immunodeficiency virus (HIV)-1 Group M, HIV-2 and HIV-1 Group O RNA, HCV RNA, and HBV DNA, for use on the cobas 6800/8800 Systems...
December 17, 2017: Transfusion
Sylvester Yao Lokpo, James Osei-Yeboah, Gameli Kwame Norgbe, Patrick Kwasi Owiafe, Felix Ayroe, Francis Abeku Ussher, Mavis Popuelle Dakorah, John Gameli Deku, Nana Yaw Barimah Manaphraim, Emmanuel Akomanin Asiamah, Tibemponi Ntoni, Prince Senyo Kwasi Nyamadi, Edward Yiadom Boakye, Roseline Avorkliyah
Background: Using prospective blood donors as a proxy, this study was aimed at estimating the burden and five-year (2012-2016) trend of viral hepatitis (HBV and HCV) infection among asymptomatic adult population in Ho. Materials and Methods: A retrospective analysis was done on secondary data extracted from the hospital archives comprising 4,180 prospective blood donors from January 2012 to December 2016. Demographic variables included age and sex, as well as place of residence...
2017: Hepatitis Research and Treatment
Laure Stella Ghoma Linguissi, Celine Nguefeu Nkenfou
OBJECTIVE: Considered an endemic zone, Republic of Congo has a high seroprevalence rate of hepatitis B and C virus. To know the extent of hepatitis infection as a public health problem, we reviewed published literature and other sources for reports of these viral infections in the country. RESULTS: High seroprevalence of HBV and HCV carriage in blood donors were observed in studies confirming Congo's place in the hyperendemic area of HBV and HCV infection. These prevalence were compared by Chi square test...
December 2, 2017: BMC Research Notes
A D Ware, C Jacquot, A A R Tobian, E A Gehrie, P M Ness, E M Bloch
Transfusion-transmitted infection risk remains an enduring challenge to blood safety in Africa. A high background incidence and prevalence of the major transfusion-transmitted infections (TTIs), dependence on high-risk donors to meet demand, suboptimal testing and quality assurance collectively contribute to the increased risk. With few exceptions, donor testing is confined to serological evaluation of human immunodeficiency virus (HIV), hepatitis B and C (HBV and HCV) and syphilis. Barriers to implementation of broader molecular methods include cost, limited infrastructure and lack of technical expertise...
November 30, 2017: Vox Sanguinis
Sylvester Yao Lokpo, Mavis Popuelle Dakorah, Gameli Kwame Norgbe, James Osei-Yeboah, Godwin Adzakpah, Isaac Sarsah, John Gameli Deku, Innocent Afeke, Emmanuel Akomanin Asiamah, Nana Yaw Barimah Manaphraim, Isaac Asare, Bright Justice Ayidzoe, Emmanuel Alote Allotey, Emmanuel Agbeko Nani, Paul Amoah
Background: This study was aimed at evaluating the seroprevalence and trend of blood-borne pathogens (HIV, HCV, HBV, and Syphilis) among asymptomatic adults at Akwatia during a four-year period (2013-2016). Materials and Methods: The study was a retrospective analysis of secondary data of blood donors who visited the hospital from January 2013 to December 2016. Archival data from 11,436 prospective donors was extracted. Data included age, sex, and place of residence as well as results of infectious markers (HIV, HBV, HCV, and Syphilis)...
2017: Journal of Tropical Medicine
Melissa A Greenwald, Stephen Kerby, Kori Francis, Anna C Noller, William T Gormley, Robin Biswas, Richard A Forshee
BACKGROUND: Evaluation of assay performance on post-mortem blood specimens (obtained after cessation of the heartbeat) presents unique scientific and regulatory challenges. In the United States, assay performance is evaluated in part by spiking postmortem specimens. METHODS: Fifty-four specimens obtained from decedents known to be infected with human immunodeficiency virus (HIV), hepatitis C virus (HCV), or hepatitis B virus (HBV), including some coinfections, were tested for each virus using Food and Drug Administration (FDA)-licensed donor screening tests for nucleic acid, antibody, and antigen...
November 27, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
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