keyword
MENU ▼
Read by QxMD icon Read
search

donors capture

keyword
https://www.readbyqxmd.com/read/28819840/microfluidic-based-enrichment-and-retrieval-of-circulating-tumor-cells-for-rt-pcr-analysis
#1
Priya Gogoi, Saedeh Sepehri, Will Chow, Kalyan Handique, Yixin Wang
Molecular analysis of circulating tumor cells (CTCs) is hindered by low sensitivity and high level of background leukocytes of currently available CTC enrichment technologies. We have developed a novel device to enrich and retrieve CTCs from blood samples by using a microfluidic chip. The Celsee PREP100 device captures CTCs with high sensitivity and allows the captured CTCs to be retrieved for molecular analysis. It uses the microfluidic chip which has approximately 56,320 capture chambers. Based on differences in cell size and deformability, each chamber ensures that small blood escape while larger CTCs of varying sizes are trapped and isolated in the chambers...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28762264/chemically-edited-exosomes-with-dual-ligand-purified-by-microfluidic-device-for-active-targeted-drug-delivery-to-tumor-cells
#2
Jie Wang, Wei Li, Leicheng Zhang, Lin Ban, Peng Chen, Wei Du, Xiaojun Feng, Bi-Feng Liu
Exosomes, which are lipid membrane-bound nanovesicles (50-150 nm in diameter), have aroused extensive attention for their potential applications in invasive molecular and stand for a new therapeutic delivery system. However, they are limited by poor targeting ability and a lack of efficient isolation techniques. Here, we present a three-dimensional nanostructured microfluidic chip, in which arrays of micropillars were functionalized with crisscrossed multiwall carbon nanotubes by chemical deposition, to capture exosomes with high efficiency through a combination of a specific recognition molecule (CD63) and the unique topography of the nanomaterials...
August 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28761338/a-review-of-fibrin-and-fibrin-composites-for-bone-tissue-engineering
#3
REVIEW
Alireza Noori, Seyed Jamal Ashrafi, Roza Vaez-Ghaemi, Ashraf Hatamian-Zaremi, Thomas J Webster
Tissue engineering has emerged as a new treatment approach for bone repair and regeneration seeking to address limitations associated with current therapies, such as autologous bone grafting. While many bone tissue engineering approaches have traditionally focused on synthetic materials (such as polymers or hydrogels), there has been a lot of excitement surrounding the use of natural materials due to their biologically inspired properties. Fibrin is a natural scaffold formed following tissue injury that initiates hemostasis and provides the initial matrix useful for cell adhesion, migration, proliferation, and differentiation...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28758700/dengue-virus-infections-among-blood-donors-in-guangxi-of-china-2013-2014
#4
Z Gao, Y Zhang, Y Yang, M Xu, P Liao, W He, J Xu, Y Liu, M He
BACKGROUND: An unexpected, large dengue outbreak, spreading to 20 cities of Guangdong province, occurred in July 2014. Considering the overwhelming influence of this seasonal epidemic, whether this dengue virus outbreak has an significant impact on the blood donors of the ajacent areas in Guangdong requires at least a preliminary investigation. Liuzhou City of Guangxi was chosen as a good representative of the neighbouring geographical location of the dengue virus (DENV) outbreak areas in this research...
July 31, 2017: Transfusion Medicine
https://www.readbyqxmd.com/read/28741109/multi-analyte-method-development-for-analysis-of-brominated-flame-retardants-bfrs-and-pbde-metabolites-in-human-serum
#5
Dasheng Lu, Yu'e Jin, Chao Feng, Dongli Wang, Yuanjie Lin, Xinlei Qiu, Qian Xu, Yimin Wen, Jianwen She, Guoquan Wang, Zhijun Zhou
Commonly, analytical methods measuring brominated flame retardants (BFRs) of different chemical polarities in human serum are labor consuming and tedious. Our study used acidified diatomaceous earth as solid-phase extraction (SPE) adsorbent and defatting material to simultaneously determine the most abundant BFRs and their metabolites with different polarities in human serum samples. The analytes include three types of commercial BFRs, tetrabromobisphenol A (TBBPA), hexabromocyclododecane (HBCD) isomers, and polybrominated biphenyl ethers (PBDEs), and dominant hydroxylated BDE (OH-PBDE) and methoxylated BDE (MeO-PBDE) metabolites of PBDEs...
July 24, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28729350/structures-of-the-crispr-genome-integration-complex
#6
Addison V Wright, Jun-Jie Liu, Gavin J Knott, Kevin W Doxzen, Eva Nogales, Jennifer A Doudna
CRISPR-Cas systems depend on the Cas1-Cas2 integrase to capture and integrate short foreign DNA fragments into the CRISPR locus, enabling adaptation to new viruses. We present crystal structures of Cas1-Cas2 bound to both donor and target DNA in intermediate and product integration complexes, as well as a cryo-electron microscopy structure of the full CRISPR locus integration complex including the accessory protein Integration Host Factor (IHF). The structures show unexpectedly that indirect sequence recognition dictates integration site selection by favoring deformation of the repeat and the flanking sequences...
July 20, 2017: Science
https://www.readbyqxmd.com/read/28726800/role-of-nicotinamide-n-methyltransferase-in-dorsal-striatum-in-cocaine-place-preference
#7
Li Luo, Fei-Fei Shang, Hailei Long, Linhong Jiang, Ruiming Zhu, Qian Zhao, Hui Gu, Jueying Kong, Wei Xu, Yinglan Zhao, Xiaobo Cen
Nicotinamide N-methyltransferase (NNMT) transfers the methyl from S-adenosyl-L-methionine (SAM) to nicotinamide (NA) to produce S-adenosyl-L-homocysteine (SAH) and 1-methylnicotinamide (MeN). NNMT has been implicated in a variety of diseases; however, the role of NNMT in drug addiction is largely unknown. Here, we found that the expression of Nnmt was significantly upregulated in the dorsal striatum (DS) of cocaine-conditioned mice. Cocaine significantly decreased SAM/SAH ratio levels in the DS, which was accompanied with the decreased activities of Rac1 and RhoA...
July 20, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28700723/position-effects-influencing-intrachromosomal-repair-of-a-double-strand-break-in-budding-yeast
#8
Ruoxi W Wang, Cheng-Sheng Lee, James E Haber
Repair of a double-strand break (DSB) by an ectopic homologous donor sequence is subject to the three-dimensional arrangement of chromosomes in the nucleus of haploid budding yeast. The data for interchromosomal recombination suggest that searching for homology is accomplished by a random collision process, strongly influenced by the contact probability of the donor and recipient sequences. Here we explore how recombination occurs on the same chromosome and whether there are additional constraints imposed on repair...
2017: PloS One
https://www.readbyqxmd.com/read/28685370/organochlorine-pesticides-levels-and-associated-factors-in-a-group-of-blood-donors-in-s%C3%A3-o-paulo-brazil
#9
Felipe Parra do Nascimento, Rúbia Kuno, Vera Regina Rossi Lemes, Tereza Atsuko Kussumi, Viviane Emi Nakano, Sonia Bio Rocha, Maria Celeste Cardeal de Oliveira, Iracema de Albuquerque Kimura, Nelson Gouveia
The use of organochlorine pesticides (OCPs) is a worldwide public health concern given that high levels of these compounds in humans and animals can lead to serious health problems. This study aimed to evaluate the levels of 15 organochlorine pesticides in the serum of 547 blood donors in the São Paulo metropolitan region (SPMR) in 2009 and to investigate factors associated with higher levels of these compounds. The OCPs were determined by gas chromatography with micro electron capture detection. Multiple ordinal logistic regression models were employed to determine the factors associated with higher levels...
August 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28675134/overview-of-the-infant-and-young-child-feeding-policy-environment-in-pakistan-federal-sindh-and-punjab-context
#10
Hana Mahmood, Yasmeen Suleman, Tabish Hazir, Durre Samin Akram, Shahadat Uddin, Michael J Dibley, Saleem Abassi, Amara Shakeel, Narjis Kazmi, Anne Marie Thow
BACKGROUND: Appropriate infant and young child feeding (IYCF) practices have been identified as important for appropriate child growth and development. (Ministry of Planning and Development, Ministry of National Health Services, Regulations and Coordination (2012)) Children in Pakistan still experience high rates of malnutrition, indicating a likely need for stronger IYCF policy. The purpose of this study was to identify major stakeholders who shape the IYCF policy environment and analyze which policies protect, promote and support IYCF practices, either directly or indirectly...
June 13, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28672190/a-novel-signal-on-photoelectrochemical-immunosensor-for-detection-of-alpha-fetoprotein-by-in-situ-releasing-electron-donor
#11
Jiexia Chen, Guang-Chao Zhao
A signal-on photoelectrochemical (PEC) immunosensor was constructed for detecting tumor marker in this work. α-fetoprotein (AFP) was chosen as a model analyte to investigate the prepared procedure and the analytical performance of the exploited sensor. In order to construct the sensor, CdSe QDs were used as photoactive material, biotin conjugated AFP antibody (Bio-anti-AFP) as detecting probe, streptavidin (SA) as signal capturing unit, biotin functionalized apoferritin encapsulated ascorbic acid (Bio-APOAA) as amplification unit, which were assembled onto the electrodes...
June 24, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28666085/effect-of-solid-state-polarization-on-charge-transfer-excitations-and-transport-levels-at-organic-interfaces-from-a-screened-range-separated-hybrid-functional
#12
Zilong Zheng, David A Egger, Jean-Luc Brédas, Leeor Kronik, Veaceslav Coropceanu
We develop a robust approach for the description of the energetics of charge-transfer (CT) excitations and transport levels at organic interfaces based on a screened range-separated hybrid (SRSH) functional. We find that SRSH functionals correctly capture the effect of solid-state electronic polarization on transport gap renormalization and on screening of the electrostatic electron-hole interaction. With respect to calculations based on nonscreened optimally tuned RSH (long-range corrected) functionals, the SRSH-based calculations can be performed for both isolated molecular complexes and systems embedded in a dielectric medium with the same range-separation parameter, which allows a clear physical interpretation of the results in terms of solid-state polarization without any perturbation of the molecular electronic structure...
July 6, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28651044/atmospheric-fate-of-monoethanolamine-enhancing-new-particle-formation-of-sulfuric-acid-as-an-important-removal-process
#13
Hong-Bin Xie, Jonas Elm, Roope Halonen, Nanna Myllys, Theo Kurtén, Markku Kulmala, Hanna Vehkamäki
Monoethanolamine (MEA), a potential atmospheric pollutant from the capture unit of a leading CO2 capture technology, could be removed by participating H2SO4-based new particle formation (NPF) as simple amines. Here we evaluated the enhancing potential of MEA on H2SO4-based NPF by examining the formation of molecular clusters of MEA and H2SO4 using combined quantum chemistry calculations and kinetics modeling. The results indicate that MEA at the parts per trillion (ppt) level can enhance H2SO4-based NPF. The enhancing potential of MEA is less than that of dimethylamine (DMA), one of the strongest enhancing agents, and much greater than methylamine (MA), in contrast to the order suggested solely by their basicity (MEA < MA < DMA)...
July 11, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28644817/graphene-based-humidity-sensors-the-origin-of-alternating-resistance-change
#14
V I Popov, D V Nikolaev, V B Timofeev, S A Smagulova, I V Antonova
The response of a graphene-based humidity sensor is considered as a function of film structures. Analysis of the resistance changes due to water molecule adsorption on the graphene or multi-layer graphene (MLG) surface is performed for films with different structures and resistivities from hundreds of ohms/sq to hundreds of kilo-ohms/sq. The results revealed possible increase, decrease and non-monotonous behavior of resistance with changes in film structure. Adsorption of water molecules at grain boundary defects is assumed to lead to an increase in film resistivity due to the donor property of water and the p-type conductivity of graphene...
June 23, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28641647/-clinical-significance-of-serum-thrombopoietin-levels-in-patients-with-aplastic-anaemia
#15
Hai Cheng, Jiang Cao, Wei Chen, Kun-Ming Qi, Zhi-Ling Yan, Kai-Lin Xu
OBJECTIVE: To evaluate the clinical significance of thrombopoietin (TPO) level in diagnosis of pateints with aplastic anaemia (AA). METHODS: The TPO levels in sera from 54 AA patients and 119 healthy controls were examined. A total of 92 samples were collected from AA patients including 43 samples harvested at diagnosis, 23 samples in the cytopenic period after treatment, and 26 samples of patients in partial (n=10) or complete remission (n=16) following immunosuppressive treatment...
June 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28637792/reactive-sulfur-species-inactivate-ca-2-calmodulin-dependent-protein-kinase-iv-via-s-polysulfidation-of-its-active-site-cysteine-residue
#16
Tsuyoshi Takata, Hideshi Ihara, Naoya Hatano, Yukihiro Tsuchiya, Takaaki Akaike, Yasuo Watanabe
Reactive sulfur species (RSS) modulate protein functions via S-polysulfidation of reactive Cys residues. Here, we report that Ca(2+)/calmodulin (CaM)-dependent protein kinase IV (CaMKIV) was reversibly inactivated by RSS via polysulfidation of the active-site Cys residue. CaMKIV is phosphorylated at Thr(196) by its upstream CaMK kinase (CaMKK), resulting in the induction of its full activity. In vitro incubation of CaMKIV with the exogenous RSS donors Na2S n (n = 2-4) resulted in dose-dependent inhibition of the CaMKK-induced phospho-Thr(196) and consequent inactivation of the enzyme activity...
July 17, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28634955/human-lung-small-airway-on-a-chip-protocol
#17
Kambez H Benam, Marc Mazur, Youngjae Choe, Thomas C Ferrante, Richard Novak, Donald E Ingber
Organs-on-chips are microfluidic cell culture devices created using microchip manufacturing techniques that contain hollow microchannels lined by living cells, which recreate specialized tissue-tissue interfaces, physical microenvironments, and vascular perfusion necessary to recapitulate organ-level physiology in vitro. Here we describe a protocol for fabrication, culture, and operation of a human lung "small airway-on-a-chip," which contains a differentiated, mucociliary bronchiolar epithelium exposed to air and an underlying microvascular endothelium that experiences fluid flow...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28614535/should-azoospermic-patients-with-varicocele-disease-undergo-surgery-to-recover-fertility
#18
Leonardo de Souza Alves, Francisco Batista de Oliveira
Introduction: Varicocele disease is well-known cause of infertility in men. The presence of spermatic varices veins create a hostile environment to spermatogenesis. It results in reduced quality of the sperm production and in some cases can determine a total absence of sperm. The varicocelectomy procedure in patients with non-obstructive azoospermia (NOA) can raise the rates of sperm in the semen analysis. A positive rate for sperm, even if very low, may be sufficient to enable the capture of sperm intended for in-vitro fertilization without the use of donor sperm...
April 2017: Revista da Associação Médica Brasileira
https://www.readbyqxmd.com/read/28600230/cytokine-and-chemokine-profile-changes-in-patients-with-lower-segment-lumbar-degenerative-spondylolisthesis
#19
Juraj Sutovsky, Martin Benco, Martina Sutovska, Michaela Kocmalova, Lenka Pappova, Juraj Miklusica, Andrej Frano, Egon Kurca
BACKGROUND: Lumbar degenerative spondylolisthesis (DS) develops as a result of inflammatory and remodeling processes in facet joints (FJs). Several inflammatory cytokines are involved in the osteoarthritic and remodeling changes that occur and in low-back and/or radicular pain, the most prevalent clinical symptom of disease. This study improves knowledge related to the roles that 27 cytokines, chemokines and growth factors play in the pathophysiology of lumbar DS. MATERIAL AND METHODS: Cytokine levels were examined using capture sandwich immunoassay using the Bio-Plex(®) 200 System and the Bio-PlexTM Human Cytokine Standard 27-Plex, Group I (Bio-Rad, Hercules, California, USA) separately in intervertebral discs (IVDs) and FJ bone tissue...
June 6, 2017: International Journal of Surgery
https://www.readbyqxmd.com/read/28589096/identification-of-s-nitrosylated-sno-proteins-in-entamoeba-histolytica-adapted-to-nitrosative-stress-insights-into-the-role-of-sno-actin-and-in-vitro-virulence
#20
Meirav Trebicz-Geffen, Preeti Shahi, Shruti Nagaraja, Shai Vanunu, Shiran Manor, Amit Avrahami, Serge Ankri
We have recently reported that Entamoeba histolytica trophozoites can adapt to toxic levels of the nitric oxide (NO) donor, S-nitrosoglutathione (GSNO). Even if the consequences of this adaptation on the modulation of gene expression in NO-adapted trophozoites (NAT) have been previously explored, insight on S-nitrosylated (SNO) proteins in NAT is missing. Our study aims to fill this knowledge gap by performing a screening of SNO proteins in NAT. Employing SNO resin-assisted capture (RAC), we identified 242 putative SNO proteins with key functions in calcium binding, enzyme modulation, redox homeostasis, and actin cytoskeleton...
2017: Frontiers in Cellular and Infection Microbiology
keyword
keyword
62781
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"