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https://www.readbyqxmd.com/read/28930983/personalized-peptide-arrays-for-detection-of-hla-alloantibodies-in-organ-transplantation
#1
Pan Liu, Tomokazu Souma, Andrew Zu-Sern Wei, Xueying Xie, Xunrong Luo, Jing Jin
In organ transplantation, the function and longevity of the graft critically rely on the success of controlling immunological rejection reactivity against human leukocyte antigens (HLA). Histocompatibility guidelines are based on laboratory tests of anti-HLA immunity, which presents either as pre-existing or de novo generated HLA antibodies that constitute a major transplantation barrier. Current tests are built on a single-antigen beads (SAB) platform using a fixed set of ~100 preselected recombinant HLA antigens to probe transplant sera...
September 6, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28881932/health-facility-management-and-access-a-qualitative-analysis-of-challenges-to-seeking-healthcare-for-children-under-five-in-uganda
#2
Elizabeth Palchik Allen, Wilson Winstons Muhwezi, Dorcus Kiwanuka Henriksson, Anthony Kabanza Mbonye
While several studies have documented the various barriers that caretakers of children under five routinely confront when seeking healthcare in Uganda, few have sought to capture the ways in which caretakers themselves prioritize their own barriers to seeking services. To that end, we asked focus groups of caretakers to list their five greatest challenges to seeking care on behalf of children under five. Using qualitative content analysis, we grouped responses according to four categories: (1) geographical access barriers; (2) facility supplies, staffing, and infrastructural barriers; (3) facility management and administration barriers (e...
September 1, 2017: Health Policy and Planning
https://www.readbyqxmd.com/read/28877242/comparative-quantitative-proteomic-analysis-of-disease-stratified-laser-captured-microdissected-human-islets-identifies-proteins-and-pathways-potentially-related-to-type-1-diabetes
#3
Julius O Nyalwidhe, Wojciech J Grzesik, Tanya C Burch, Michele L Semeraro, Tayab Waseem, Ivan C Gerling, Raghavendra G Mirmira, Margaret A Morris, Jerry L Nadler
Type 1 diabetes (T1D) is a chronic inflammatory disease that is characterized by autoimmune destruction of insulin-producing pancreatic beta cells. The goal of this study was to identify novel protein signatures that distinguish Islets from patients with T1D, patients who are autoantibody positive without symptoms of diabetes, and from individuals with no evidence of disease. High resolution high mass accuracy label free quantitative mass spectrometry analysis was applied to islets isolated by laser capture microdissection from disease stratified human pancreata from the Network for Pancreatic Organ Donors with Diabetes (nPOD), these included donors without diabetes, donors with T1D-associated autoantibodies in the absence of diabetes, and donors with T1D...
2017: PloS One
https://www.readbyqxmd.com/read/28873423/an-immunosignature-test-distinguishes-trypanosoma-cruzi-hepatitis-b-hepatitis-c-and-west-nile-virus-seropositivity-among-asymptomatic-blood-donors
#4
Michael Rowe, Jonathan Melnick, Robert Gerwien, Joseph B Legutki, Jessica Pfeilsticker, Theodore M Tarasow, Kathryn F Sykes
BACKGROUND: The complexity of the eukaryotic parasite Trypanosoma (T.) cruzi manifests in its highly dynamic genome, multi-host life cycle, progressive morphologies and immune-evasion mechanisms. Accurate determination of infection or Chagas' disease activity and prognosis continues to challenge researchers. We hypothesized that a diagnostic platform with higher ligand complexity than previously employed may hold value. METHODOLOGY: We applied the ImmunoSignature Technology (IST) for the detection of T...
September 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28862274/high-purity-capture-and-release-of-circulating-exosomes-using-an-exosome-specific-dual-patterned-immunofiltration-exodif-device
#5
Yoon-Tae Kang, Young Jun Kim, Jiyoon Bu, Young-Ho Cho, Sae-Won Han, Byung-In Moon
We present a microfluidic device for the capture and release of circulating exosomes from human blood. The exosome-specific dual-patterned immunofiltration (ExoDIF) device is composed of two distinct immuno-patterned layers, and is capable of enhancing the chance of binding between the antibody and exosomes by generating mechanical whirling, thus achieving high-throughput exosome isolation with high specificity. Moreover, follow-up recovery after the immuno-affinity based isolation, via cleavage of a linker, enables further downstream analysis...
September 1, 2017: Nanoscale
https://www.readbyqxmd.com/read/28853609/in%C3%A2-vitro-development-of-personalized-cartilage-microtissues-uncovers-an-individualized-differentiation-capacity-of-human-chondrocytes
#6
Frank Martin, Mario Lehmann, Ulrich Sack, Ursula Anderer
Personalized features in the treatment of knee injuries and articular replacement therapies play an important role in modern life with increasing demand. Therefore, cell-based therapeutic approaches for the regeneration of traumatic defects of cartilage tissue were developed. However, great variations in the quality of repair tissue or therapeutic outcome were observed. The aim of the study was to capture and visualize individual differentiation capacities of chondrocytes derived from different donors with regard to a possible personal regeneration capacity using a cell-based therapy...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28837583/single-cell-profiling-of-dynamic-cytokine-secretion-and-the-phenotype-of-immune-cells
#7
Xingyue An, Victor G Sendra, Ivan Liadi, Balakrishnan Ramesh, Gabrielle Romain, Cara Haymaker, Melisa Martinez-Paniagua, Yanbin Lu, Laszlo G Radvanyi, Badrinath Roysam, Navin Varadarajan
Natural killer (NK) cells are a highly heterogeneous population of innate lymphocytes that constitute our first line of defense against several types of tumors and microbial infections. Understanding the heterogeneity of these lymphocytes requires the ability to integrate their underlying phenotype with dynamic functional behaviors. We have developed and validated a single-cell methodology that integrates cellular phenotyping and dynamic cytokine secretion based on nanowell arrays and bead-based molecular biosensors...
2017: PloS One
https://www.readbyqxmd.com/read/28821604/development-of-a-multiplexed-assay-for-oral-cancer-candidate-biomarkers-using-peptide-immunoaffinity-enrichment-and-targeted-mass-spectrometry
#8
Yung-Chin Hsiao, Lang-Ming Chi, Kun-Yi Chien, Wei-Fan Chiang, Szu-Fan Chen, Yao-Ning Chuang, Shih-Yu Lin, Chia-Chun Wu, Ya-Ting Chang, Lichieh Julie Chu, Yi-Ting Chen, Shu-Li Chia, Chih-Yen Chien, Kai-Ping Chang, Yu-Sun Chang, Jau-Song Yu
Oral cancer is one of the most common cancers worldwide, and there are currently no biomarkers approved for aiding its management. Although many potential oral cancer biomarkers have been discovered, very few have been verified in body fluid specimens in parallel to evaluate their clinical utility. The lack of appropriate multiplexed assays for chosen targets represents one of the bottlenecks to achieving this goal. In the present study, we develop a peptide immunoaffinity enrichment-coupled multiple reaction monitoring-mass spectrometry (SISCAPA-MRM) assay for verifying multiple reported oral cancer biomarkers in saliva...
August 18, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28819840/microfluidic-based-enrichment-and-retrieval-of-circulating-tumor-cells-for-rt-pcr-analysis
#9
Priya Gogoi, Saedeh Sepehri, Will Chow, Kalyan Handique, Yixin Wang
Molecular analysis of circulating tumor cells (CTCs) is hindered by low sensitivity and high level of background leukocytes of currently available CTC enrichment technologies. We have developed a novel device to enrich and retrieve CTCs from blood samples by using a microfluidic chip. The Celsee PREP100 device captures CTCs with high sensitivity and allows the captured CTCs to be retrieved for molecular analysis. It uses the microfluidic chip which has approximately 56,320 capture chambers. Based on differences in cell size and deformability, each chamber ensures that small blood escape while larger CTCs of varying sizes are trapped and isolated in the chambers...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28762264/chemically-edited-exosomes-with-dual-ligand-purified-by-microfluidic-device-for-active-targeted-drug-delivery-to-tumor-cells
#10
Jie Wang, Wei Li, Leicheng Zhang, Lin Ban, Peng Chen, Wei Du, Xiaojun Feng, Bi-Feng Liu
Exosomes, which are lipid membrane-bound nanovesicles (50-150 nm in diameter), have aroused extensive attention for their potential applications in invasive molecular and stand for a new therapeutic delivery system. However, they are limited by poor targeting ability and a lack of efficient isolation techniques. Here, we present a three-dimensional nanostructured microfluidic chip, in which arrays of micropillars were functionalized with crisscrossed multiwall carbon nanotubes by chemical deposition, to capture exosomes with high efficiency through a combination of a specific recognition molecule (CD63) and the unique topography of the nanomaterials...
August 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28761338/a-review-of-fibrin-and-fibrin-composites-for-bone-tissue-engineering
#11
REVIEW
Alireza Noori, Seyed Jamal Ashrafi, Roza Vaez-Ghaemi, Ashraf Hatamian-Zaremi, Thomas J Webster
Tissue engineering has emerged as a new treatment approach for bone repair and regeneration seeking to address limitations associated with current therapies, such as autologous bone grafting. While many bone tissue engineering approaches have traditionally focused on synthetic materials (such as polymers or hydrogels), there has been a lot of excitement surrounding the use of natural materials due to their biologically inspired properties. Fibrin is a natural scaffold formed following tissue injury that initiates hemostasis and provides the initial matrix useful for cell adhesion, migration, proliferation, and differentiation...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28758700/dengue-virus-infections-among-blood-donors-in-guangxi-of-china-2013-2014
#12
Z Gao, Y Zhang, Y Yang, M Xu, P Liao, W He, J Xu, Y Liu, M He
BACKGROUND: An unexpected, large dengue outbreak, spreading to 20 cities of Guangdong province, occurred in July 2014. Considering the overwhelming influence of this seasonal epidemic, whether this dengue virus outbreak has an significant impact on the blood donors of the ajacent areas in Guangdong requires at least a preliminary investigation. Liuzhou City of Guangxi was chosen as a good representative of the neighbouring geographical location of the dengue virus (DENV) outbreak areas in this research...
July 31, 2017: Transfusion Medicine
https://www.readbyqxmd.com/read/28741109/multi-analyte-method-development-for-analysis-of-brominated-flame-retardants-bfrs-and-pbde-metabolites-in-human-serum
#13
Dasheng Lu, Yu'e Jin, Chao Feng, Dongli Wang, Yuanjie Lin, Xinlei Qiu, Qian Xu, Yimin Wen, Jianwen She, Guoquan Wang, Zhijun Zhou
Commonly, analytical methods measuring brominated flame retardants (BFRs) of different chemical polarities in human serum are labor consuming and tedious. Our study used acidified diatomaceous earth as solid-phase extraction (SPE) adsorbent and defatting material to simultaneously determine the most abundant BFRs and their metabolites with different polarities in human serum samples. The analytes include three types of commercial BFRs, tetrabromobisphenol A (TBBPA), hexabromocyclododecane (HBCD) isomers, and polybrominated biphenyl ethers (PBDEs), and dominant hydroxylated BDE (OH-PBDE) and methoxylated BDE (MeO-PBDE) metabolites of PBDEs...
July 24, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28729350/structures-of-the-crispr-genome-integration-complex
#14
Addison V Wright, Jun-Jie Liu, Gavin J Knott, Kevin W Doxzen, Eva Nogales, Jennifer A Doudna
CRISPR-Cas systems depend on the Cas1-Cas2 integrase to capture and integrate short foreign DNA fragments into the CRISPR locus, enabling adaptation to new viruses. We present crystal structures of Cas1-Cas2 bound to both donor and target DNA in intermediate and product integration complexes, as well as a cryo-electron microscopy structure of the full CRISPR locus integration complex, including the accessory protein IHF (integration host factor). The structures show unexpectedly that indirect sequence recognition dictates integration site selection by favoring deformation of the repeat and the flanking sequences...
September 15, 2017: Science
https://www.readbyqxmd.com/read/28726800/role-of-nicotinamide-n-methyltransferase-in-dorsal-striatum-in-cocaine-place-preference
#15
Li Luo, Fei-Fei Shang, Hailei Long, Linhong Jiang, Ruiming Zhu, Qian Zhao, Hui Gu, Jueying Kong, Wei Xu, Yinglan Zhao, Xiaobo Cen
Nicotinamide N-methyltransferase (NNMT) transfers the methyl from S-adenosyl-L-methionine (SAM) to nicotinamide (NA) to produce S-adenosyl-L-homocysteine (SAH) and 1-methylnicotinamide (MeN). NNMT has been implicated in a variety of diseases; however, the role of NNMT in drug addiction is largely unknown. Here, we found that the expression of Nnmt was significantly upregulated in the dorsal striatum (DS) of cocaine-conditioned mice. Cocaine significantly decreased SAM/SAH ratio levels in the DS, which was accompanied with the decreased activities of Rac1 and RhoA...
July 20, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28700723/position-effects-influencing-intrachromosomal-repair-of-a-double-strand-break-in-budding-yeast
#16
Ruoxi W Wang, Cheng-Sheng Lee, James E Haber
Repair of a double-strand break (DSB) by an ectopic homologous donor sequence is subject to the three-dimensional arrangement of chromosomes in the nucleus of haploid budding yeast. The data for interchromosomal recombination suggest that searching for homology is accomplished by a random collision process, strongly influenced by the contact probability of the donor and recipient sequences. Here we explore how recombination occurs on the same chromosome and whether there are additional constraints imposed on repair...
2017: PloS One
https://www.readbyqxmd.com/read/28685370/organochlorine-pesticides-levels-and-associated-factors-in-a-group-of-blood-donors-in-s%C3%A3-o-paulo-brazil
#17
Felipe Parra do Nascimento, Rúbia Kuno, Vera Regina Rossi Lemes, Tereza Atsuko Kussumi, Viviane Emi Nakano, Sonia Bio Rocha, Maria Celeste Cardeal de Oliveira, Iracema de Albuquerque Kimura, Nelson Gouveia
The use of organochlorine pesticides (OCPs) is a worldwide public health concern given that high levels of these compounds in humans and animals can lead to serious health problems. This study aimed to evaluate the levels of 15 organochlorine pesticides in the serum of 547 blood donors in the São Paulo metropolitan region (SPMR) in 2009 and to investigate factors associated with higher levels of these compounds. The OCPs were determined by gas chromatography with micro electron capture detection. Multiple ordinal logistic regression models were employed to determine the factors associated with higher levels...
August 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28675134/overview-of-the-infant-and-young-child-feeding-policy-environment-in-pakistan-federal-sindh-and-punjab-context
#18
Hana Mahmood, Yasmeen Suleman, Tabish Hazir, Durre Samin Akram, Shahadat Uddin, Michael J Dibley, Saleem Abassi, Amara Shakeel, Narjis Kazmi, Anne Marie Thow
BACKGROUND: Appropriate infant and young child feeding (IYCF) practices have been identified as important for appropriate child growth and development. (Ministry of Planning and Development, Ministry of National Health Services, Regulations and Coordination (2012)) Children in Pakistan still experience high rates of malnutrition, indicating a likely need for stronger IYCF policy. The purpose of this study was to identify major stakeholders who shape the IYCF policy environment and analyze which policies protect, promote and support IYCF practices, either directly or indirectly...
June 13, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28672190/a-novel-signal-on-photoelectrochemical-immunosensor-for-detection-of-alpha-fetoprotein-by-in-situ-releasing-electron-donor
#19
Jiexia Chen, Guang-Chao Zhao
A signal-on photoelectrochemical (PEC) immunosensor was constructed for detecting tumor marker in this work. α-fetoprotein (AFP) was chosen as a model analyte to investigate the prepared procedure and the analytical performance of the exploited sensor. In order to construct the sensor, CdSe QDs were used as photoactive material, biotin conjugated AFP antibody (Bio-anti-AFP) as detecting probe, streptavidin (SA) as signal capturing unit, biotin functionalized apoferritin encapsulated ascorbic acid (Bio-APOAA) as amplification unit, which were assembled onto the electrodes...
June 24, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28666085/effect-of-solid-state-polarization-on-charge-transfer-excitations-and-transport-levels-at-organic-interfaces-from-a-screened-range-separated-hybrid-functional
#20
Zilong Zheng, David A Egger, Jean-Luc Brédas, Leeor Kronik, Veaceslav Coropceanu
We develop a robust approach for the description of the energetics of charge-transfer (CT) excitations and transport levels at organic interfaces based on a screened range-separated hybrid (SRSH) functional. We find that SRSH functionals correctly capture the effect of solid-state electronic polarization on transport gap renormalization and on screening of the electrostatic electron-hole interaction. With respect to calculations based on nonscreened optimally tuned RSH (long-range corrected) functionals, the SRSH-based calculations can be performed for both isolated molecular complexes and systems embedded in a dielectric medium with the same range-separation parameter, which allows a clear physical interpretation of the results in terms of solid-state polarization without any perturbation of the molecular electronic structure...
July 6, 2017: Journal of Physical Chemistry Letters
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