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https://www.readbyqxmd.com/read/28439930/detection-and-identification-of-platelet-antibodies-using-a-sensitive-multiplex-assay-system-platelet-antibody-bead-array
#1
Krista Metzner, Julie Bauer, Heather Ponzi, Allison Ujcich, Brian R Curtis
BACKGROUND: Tests for platelet-specific antibodies are important in the diagnosis of immune platelet disorders. Monoclonal antibody glycoprotein capture assays have been the gold standards in platelet antibody detection for almost 30 years. However, such assays are complex and cumbersome to perform, which limits their routine use. We report the performance of a newly developed, easy to perform platelet antibody bead array (PABA) for the detection of platelet-specific antibodies. STUDY DESIGN AND METHODS: PABA is the equivalent of the monoclonal antigen capture enzyme-linked immunosorbent assay (ELISA) (MACE) on a bead and instead with fluorescent detection of immunoglobulin (Ig)G platelet antibodies by Luminex...
April 25, 2017: Transfusion
https://www.readbyqxmd.com/read/28421672/a-transplant-specific-quality-initiative-introducing-transqip-a-joint-effort-of-the-asts-and-acs
#2
Justin Parekh, Clifford Ko, Jacqueline Lappin, Stuart Greenstein, Ryutaro Hirose
In an attempt to improve surgical quality in the field of transplantation, the American College of Surgeons (ACS) and American Society and Transplant Surgeons have initiated a national quality improvement program in transplantation. This transplant specific quality improvement program, called TransQIP, has been built from the ground up by transplant surgeons and captures detailed information on donor and recipient factors as well as transplant specific outcomes. It is built upon the existing ACS/NSQIP infrastructure and is designed to capture 100% of liver and kidney transplants performed at participating sites...
April 19, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28412553/native-and-solubilized-decellularized-extracellular-matrix-a-critical-assessment-of-their-potential-for-improving-the-expansion-of-mesenchymal-stem-cells
#3
REVIEW
Aida Shakouri-Motlagh, Andrea J O'Connor, Shaun P Brennecke, Bill Kalionis, Daniel E Heath
Capturing the promise of mesenchymal stem cell (MSC)-based treatments is currently limited by inefficient production of cells needed for clinical therapies. During conventional ex vivo expansion, a large portion of MSCs lose the properties that make them attractive for use in cell therapies. Decellularized extracellular matrix (dECM) has recently emerged as a promising substrate for the improved expansion of MSCs. MSCs cultured on these surfaces exhibit improved proliferation capacity, maintenance of phenotype, and increased differentiation potential...
April 12, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28391866/nano-encapsulant-of-ascorbic-acid-loaded-apoferritin-assisted-photoelectrochemical-sensor-for-protease-detection
#4
Jiexia Chen, Guang-Chao Zhao
A signal-on photoelectrochemical (PEC) biosensor based on nano-encapsulant of ascorbic acid-loaded apoferritin-assisted for protease detection is described. The loaded ascorbic acid could be released from the central cavity of apoferritin into the buffer solution as sacrificial electron donor to capture the photo-generated holes of CdTe quantum dots when light is turned on. In this system, the biosensor relied on monitoring the photocurrent intensity as a result of enzymatic substrate proteolysis in the homogeneous aqueous solution...
June 1, 2017: Talanta
https://www.readbyqxmd.com/read/28388032/a-microporous-amic-acid-polymer-for-enhanced-ammonia-capture
#5
Ji-Woong Lee, Gokhan Barin, Gregory W Peterson, Jun Xu, Kristen A Colwell, Jeffrey R Long
Amic acids, consisting of carboxylic acids and amides, are often utilized as intermediates that can further undergo a dehydration-cyclization step to yield polymeric cyclic imides. Compared with imide-based materials, the presence of Brønsted acidic groups and multiple hydrogen-bond donors and acceptors in materials incorporating amic acids opens up the possibility for a variety of host-guest interactions. Here we report a facile and catalyst-free synthesis of a Brønsted acidic porous poly(amic acid) (PAA) and present its NH3 uptake properties using gas adsorption and breakthrough measurements...
April 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28381242/the-150-most-important-questions-in-cancer-research-and-clinical-oncology-series-questions-15-24-edited-by-chinese-journal-of-cancer
#6
EDITORIAL
(no author information available yet)
To accelerate our endeavors to overcome cancer, Chinese Journal of Cancer has launched a program of publishing 150 most important questions in cancer research and clinical oncology. In this article, 10 more questions are presented as follows. Question 15: Can tumor-induced erythrogenesis provide qualified red blood cells for carrying oxygen to distant organs? Question 16: Can we overcome tumor resistance to platinum-containing antineoplastic drugs by activating the sensitivity factors in the tumor? Question 17: How can a cancer cell stay dormant for years? Question 18: Why do cancer cells use distinct transcriptomic and proteomic programs to reach the same metastatic phenotype? Question 19: Why do some cancers regress spontaneously? Question 20: What are the regulatory mechanisms occurring in donor cells that determine selective sorting of biological content into vesicles and their biological consequences in recipient cells? Are the genetic transfer and exchange of biological messages between cells transient? Is the phenotypic manipulation of recipient cells temporary or prolonged and persistent? If extracellular vesicles possess immune-modulatory potential, how could they be exploited for immune interventions and cancer immunotherapy? Presumably the cargo of extracellular vesicles reflects the cells of their origin and can be used for cancer diagnosis, how could the uniform/stringent capture criteria be met universally for applying EVs in point-of-care diagnostics for cancer patients? Question 21: Can we use self-sampling technologies to monitor the tumor genetic alterations for more precise targeted therapy? Can we cure a heterogeneous tumor by sequentially targeting the driver molecules? Question 22: Can we postpone the onset of non-infection-related cancers? Question 23: How many types of cells can jointly form the tumor vasculature to provide blood supply for tumor progression? Question 24: How tumor cells transmit their epigenetic features to daughter cells and maintain the malignant phenotype?...
April 5, 2017: Chinese Journal of Cancer
https://www.readbyqxmd.com/read/28374831/isolation-of-circulating-plasma-cells-in-multiple-myeloma-using-cd138-antibody-based-capture-in-a-microfluidic-device
#7
Mohammad A Qasaimeh, Yichao C Wu, Suman Bose, Anoop Menachery, Srikanth Talluri, Gabriel Gonzalez, Mariateresa Fulciniti, Jeffrey M Karp, Rao H Prabhala, Rohit Karnik
The necessity for bone marrow aspiration and the lack of highly sensitive assays to detect residual disease present challenges for effective management of multiple myeloma (MM), a plasma cell cancer. We show that a microfluidic cell capture based on CD138 antigen, which is highly expressed on plasma cells, permits quantitation of rare circulating plasma cells (CPCs) in blood and subsequent fluorescence-based assays. The microfluidic device is based on a herringbone channel design, and exhibits an estimated cell capture efficiency of ~40-70%, permitting detection of <10 CPCs/mL using 1-mL sample volumes, which is difficult using existing techniques...
April 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28364926/transforming-vaccines-supply-chains-in-nigeria
#8
David Sarley, Mustafa Mahmud, Jide Idris, Modele Osunkiyesi, Onome Dibosa-Osadolor, Peter Okebukola, Owens Wiwa
Nigeria is the most populous country in Africa and in 2012 was suffering some of the lowest vaccination rates in the World. A combination of factors had resulted in a dysfunctional immunization cold chain and supply chain. Recognizing that the number of unimmunized children contributed to high levels of under-5-mortality, and that health MDGs would not be attained, Minister of State for Health Mohammed Pate launched a vaccines transformation project in 2013. In partnership with BMGF, GAVI, UNICEF, WHO, other donors and implementing partners the transformation journey has so far taken three years and achieved impressive results...
April 19, 2017: Vaccine
https://www.readbyqxmd.com/read/28362669/retrospective-assessment-of-follicular-unit-density-in-asian-men-with-androgenetic-alopecia
#9
Dell Kristie A Ortega-Castillejos, Damkerng Pathomvanich
BACKGROUND: Hair density, together with hair diameter has a tremendous impact on the cosmetic outcome of hair transplantation surgery. OBJECTIVE: This retrospective study aims to assess the average number of follicular unit (FU) grafts in the donor area of Asian men with androgenetic alopecia. METHODS: Seventy patients (ages 24-65 years old) with virgin scalp who had their first hair transplantation were included. During the harvesting procedure, a 2- to 3-cm-wide area of the donor site was shaved from ear to ear with 5 specific areas assigned as reference points for the study namely the central occipital area, left and right parietal and temporal areas...
May 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28351338/dbm-as-a-carrier-for-bmp-2-burst-release-combined-with-long-term-binding-and-osteoinductive-activity-evaluated-in-vitro-and-in-vivo
#10
Elisabeth Huber, Anne-Marie Pobloth, Nicole Bormann, Nicolai Kolarczik, Katharina Schmidt-Bleek, Hanna Schell, Philipp Schwabe, Georg Duda, Britt Wildemann
To allow bone defect regeneration, autologous bone grafting still represents the gold standard. However, autograft harvesting has limitations including an additional surgery, donor site morbidity and limited availability. Demineralized bone matrix (DBM) would represent an alternative, yet lacks sufficient osteoinductive properties. Combining DBM with a potent agent such as bone morphogenetic protein-2 (BMP-2) might be a feasible alternative approach, optimizing an established grafting material with strong osteoinductive properties...
March 29, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28351221/understanding-the-spatiotemporal-degradation-behavior-of-aggrecanase-sensitive-poly-ethylene-glycol-hydrogels-for-use-in-cartilage-tissue-engineering
#11
Stanley Chu, Shankar Lalitha Sridhar, Stacey C Skaalure, Franck J Vernerey, Stephanie J Bryant
Enzyme-sensitive hydrogels are promising cell delivery vehicles for cartilage tissue engineering. However, a better understanding of their spatiotemporal degradation behavior and its impact on tissue growth is needed. The goal of this study is to combine experimental and computational approaches to provide new insights into spatiotemporal changes in hydrogel crosslink density and extracellular matrix (ECM) growth and how these changes influence the evolving macroscopic properties as a function of time. Hydrogels were designed from aggrecanase-sensitive peptide crosslinks using a simple and robust thiol:norbornene photoclick reaction...
March 29, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28345006/genome-wide-specificity-of-highly-efficient-talens-and-crispr-cas9-for-t-cell-receptor-modification
#12
Friederike Knipping, Mark J Osborn, Karl Petri, Jakub Tolar, Hanno Glimm, Christof von Kalle, Manfred Schmidt, Richard Gabriel
In T cells with transgenic high-avidity T cell receptors (TCRs), endogenous and transferred TCR chains compete for surface expression and may pair inappropriately, potentially causing autoimmunity. To knock out endogenous TCR expression, we assembled 12 transcription activator-like effector nucleases (TALENs) and five guide RNAs (gRNAs) from the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas9) system. Using TALEN mRNA, TCR knockout was successful in up to 81% of T cells...
March 17, 2017: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/28314239/likelihood-ratio-and-posterior-odds-in-forensic-genetics-two-sides-of-the-same-coin
#13
Amke Caliebe, Susan Walsh, Fan Liu, Manfred Kayser, Michael Krawczak
It has become widely accepted in forensics that, owing to a lack of sensible priors, the evidential value of matching DNA profiles in trace donor identification or kinship analysis is most sensibly communicated in the form of a likelihood ratio (LR). This restraint does not abate the fact that the posterior odds (PO) would be the preferred basis for returning a verdict. A completely different situation holds for Forensic DNA Phenotyping (FDP), which is aimed at predicting externally visible characteristics (EVCs) of a trace donor from DNA left behind at the crime scene...
March 6, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28256086/males-without-apparent-alloimmunization-could-have-hla-antibodies-that-recognize-target-hla-specificities-expressed-on-cells
#14
J Nakamura, F Nakajima, H Kamada, K Tadokoro, T Nagai, M Satake
BACKGROUND AND OBJECTIVES: Human leukocyte antigen (HLA) antibodies, which are involved in the development of transfusion-related side effects such as transfusion-related lung injury, are sometimes found in males without a history of alloimmunization (eg, transplantation and transfusion). Whether HLA antibodies in male donors can interact with their target HLA specificities expressed on cells have not been completely investigated. MATERIALS AND METHODS: The HLA antibodies detected in 7 male donors were characterized...
May 2017: HLA
https://www.readbyqxmd.com/read/28252969/in-vitro-and-in-silico-tools-to-assess-extent-of-cellular-uptake-and-lysosomal-sequestration-of-respiratory-drugs-in-human-alveolar-macrophages
#15
Ayşe Ufuk, Frauke Assmus, Laura Francis, Jonathan Plumb, Valeriu Damian, Michael Gertz, J Brian Houston, Aleksandra Galetin
Accumulation of respiratory drugs in human alveolar macrophages (AMs) has not been extensively studied in vitro and in silico despite its potential impact on therapeutic efficacy and/or occurrence of phospholipidosis. The current study aims to characterize the accumulation and subcellular distribution of drugs with respiratory indication in human AMs and to develop an in silico mechanistic AM model to predict lysosomal accumulation of investigated drugs. The data set included 9 drugs previously investigated in rat AM cell line NR8383...
April 3, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28252345/evidence-of-stress-in-%C3%AE-cells-obtained-with-laser-capture-microdissection-from-pancreases-of-brain-dead-donors
#16
Aref Ebrahimi, Min-Ho Jung, Jonathan M Dreyfuss, Hui Pan, Dennis Sgroi, Susan Bonner-Weir, Gordon C Weir
Isolated islets used for transplantation are known to be stressed, which can result from the circumstances of death, in particular brain death, the preservation of the pancreas with its warm and cold ischemia, from the trauma of the isolation process, and the complex events that occur during tissue culture. The current study focused upon the events that occur before the islet isolation procedure. Pancreases were obtained from brain dead donors (n = 7) with mean age 50 (11) and normal pancreatic tissue obtained at surgery done for pancreatic neoplasms (n = 7), mean age 69 (9)...
March 4, 2017: Islets
https://www.readbyqxmd.com/read/28249957/a-case-based-analysis-of-whether-living-related-donors-listed-for-transplant-share-esrd-causes-with-their-recipients
#17
Arthur J Matas, Rebecca E Hays, Hassan N Ibrahim
BACKGROUND AND OBJECTIVES: Two recent studies reported increased risk of ESRD after kidney donation. In both, the majority of ESRD was seen in those donating to a relative. Confounding this observation is that, in the absence of donation, relatives of those with ESRD are at increased risk for ESRD. Understanding the pathogenesis and risk factors for postdonation ESRD is critical for both donor selection and counseling. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: We hypothesized that if familial relationship was an important consideration in pathogenesis, the donor and linked recipient would share ESRD etiology...
April 3, 2017: Clinical Journal of the American Society of Nephrology: CJASN
https://www.readbyqxmd.com/read/28248094/a-multidimensional-view-of-charge-transfer-excitons-at-organic-donor-acceptor-interfaces
#18
Ti Wang, Tika R Kafle, Bhupal Kattel, Wai-Lun Chan
How tightly bound charge transfer (CT) excitons dissociate at organic donor-acceptor interfaces has been a long-standing question in the organic photovoltaics community. Recently, it has been proposed that exciton delocalization reduces the exciton binding energy and promotes exciton dissociation. In order to understand this mechanism, it is critical to resolve the evolution of the exciton's binding energy and coherent size with femtosecond time resolution. However, because the coherent size is just a few nanometers, it presents a major experimental challenge to capture the CT process simultaneously in the energy, spatial, and temporal domains...
March 10, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28238510/piperazines-as-nootropic-agents-new-derivatives-of-the-potent-cognition-enhancer-dm235-carrying-hydrophilic-substituents
#19
Maria Vittoria Martino, Luca Guandalini, Lorenzo Di Cesare Mannelli, Marta Menicatti, Gianluca Bartolucci, Silvia Dei, Dina Manetti, Elisabetta Teodori, Carla Ghelardini, Maria Novella Romanelli
The piperazine ring of the potent nootropic drug DM235 has been decorated with H-bond donor and acceptor groups (CH2OH, CH2OMe, CH2OCOMe, COOEt); the aim was to insert new functional groups, suitable for further chemical manipulation. The influence of these modifications on nootropic activity was assessed by means of the mouse passive avoidance test; some of the newly synthesized molecules (alcohol 7b, acetate 8b and ester 10d) showed interesting in vivo potency. This makes it possible to use these functional groups for adding other residues, in order to increase molecular diversity, or for anchoring a biotin group, to obtain compounds useful to capture the biological target...
March 15, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28223278/impact-of-genetic-alterations-in-stem-cell-transplantation-for-myelodysplasia-and-secondary-acute-myeloid-leukemia
#20
Tetsuichi Yoshizato, Yasuhito Nannya, Yoshiko Atsuta, Yusuke Shiozawa, Yuka Iijima-Yamashita, Kenichi Yoshida, Yuichi Shiraishi, Hiromichi Suzuki, Yasunobu Nagata, Yusuke Sato, Nobuyuki Kakiuchi, Keitaro Matsuo, Makoto Onizuka, Keisuke Kataoka, Kenichi Chiba, Hiroko Tanaka, Hiroo Ueno, Masahiro M Nakagawa, Bartlomiej Przychodzen, Claudia Haferlach, Wolfgang Kern, Kosuke Aoki, Hidehiro Itonaga, Yoshinobu Kanda, Mikkael A Sekeres, Jaroslaw P Maciejewski, Torsten Haferlach, Yasushi Miyazaki, Keizo Horibe, Masashi Sanada, Satoru Miyano, Hideki Makishima, Seishi Ogawa
Genetic alterations, including mutations and copy number alterations, are central to the pathogenesis of myelodysplastic syndromes and related diseases (myelodysplasia), but their roles in allogeneic stem-cell transplantation have not fully been studied in a large cohort of patients. We enrolled 797 patients who had been diagnosed with myelodysplasia at initial presentation and received transplantation via the Japan Marrow Donor Program. Targeted-capture sequencing was performed to identify mutations in 69 genes, together with copy number alterations, whose effects on transplantation outcomes were investigated...
February 21, 2017: Blood
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