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https://www.readbyqxmd.com/read/29780556/integration-of-isothermal-amplification-with-quantum-dot-based-fluorescence-resonance-energy-transfer-for-simultaneous-detection-of-multiple-micrornas
#1
Juan Hu, Ming-Hao Liu, Chun-Yang Zhang
MicroRNAs (miRNAs) are small non-coding RNAs that regulate important physiological processes, and their dysregulation is associated with various human diseases. Simultaneous sensitive detection of multiple miRNAs may facilitate early clinical diagnosis. In this research, we demonstrate for the first time the integration of hyperbranched rolling circle amplification (HRCA) with quantum dot (QD)-based fluorescence resonance energy transfer (FRET) for the simultaneous detection of multiple microRNAs with a single-color QD as the donor and two fluorescent dyes as the acceptors...
May 14, 2018: Chemical Science
https://www.readbyqxmd.com/read/29779371/detection-of-nucleic-acids-in-complex-samples-via-magnetic-microbead-assisted-catalyzed-hairpin-assembly-and-dd-a-fret
#2
Hongmei Fang, Nuli Xie, Min Ou, Jin Huang, Wenshan Li, Qing Wang, Jianbo Liu, Xiaohai Yang, Kemin Wang
Nucleic acids, as one kind of significant biomarkers, have attracted tremendous attention and exhibited immense value in fundamental studies and clinical applications. In this work, we developed a fluorescent assay for detecting nucleic acids in complex samples based on magnetic microbead (MMB)-assisted catalyzed hairpin assembly (CHA) and donor donor-acceptor fluorescence resonance energy transfer ("DD-A" FRET) signaling mechanism. Three types of DNA hairpin probes were employed in this system, including Capture, H1 (double FAM-labelled probe as FRET donor) and H2 (TAMRA-labelled probe as FRET acceptor)...
May 21, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29775134/csf-inflammatory-biomarkers-responsive-to-treatment-in-progressive-multiple-sclerosis-capture-residual-inflammation-associated-with-axonal-damage
#3
Jeppe Romme Christensen, Mika Komori, Marina Rode von Essen, Rikke Ratzer, Lars Börnsen, Bibi Bielekova, Finn Sellebjerg
BACKGROUND: Development of treatments for progressive multiple sclerosis (MS) is challenged by the lack of sensitive and treatment-responsive biomarkers of intrathecal inflammation. OBJECTIVE: To validate the responsiveness of cerebrospinal fluid (CSF) inflammatory biomarkers to treatment with natalizumab and methylprednisolone in progressive MS and to examine the relationship between CSF inflammatory and tissue damage biomarkers. METHODS: CSF samples from two open-label phase II trials of natalizumab and methylprednisolone in primary and secondary progressive MS...
May 1, 2018: Multiple Sclerosis: Clinical and Laboratory Research
https://www.readbyqxmd.com/read/29774071/molecular-detection-and-analysis-of-exosomes-using-surface-enhanced-raman-scattering-gold-nanorods-and-a-miniaturized-device
#4
Elyahb Allie Kwizera, Ryan O'Connor, Vojtech Vinduska, Melody Williams, Elizabeth R Butch, Scott E Snyder, Xiang Chen, Xiaohua Huang
Exosomes are a potential source of cancer biomarkers. Probing tumor-derived exosomes can offer a potential non-invasive way to diagnose cancer, assess cancer progression, and monitor treatment responses. Novel molecular methods would facilitate exosome analysis and accelerate basic and clinical exosome research. Methods: A standard gold-coated glass microscopy slide was used to develop a miniaturized affinity-based device to capture exosomes in a target-specific manner with the assistance of low-cost 3-D printing technology...
2018: Theranostics
https://www.readbyqxmd.com/read/29770457/abo-genotyping-with-next-generation-sequencing-to-resolve-heterogeneity-in-donors-with-serology-discrepancies
#5
Ping Chun Wu, Yin-Hung Lin, Lei Fang Tsai, Ming Hung Chen, Pei-Lung Chen, Shun-Chung Pai
BACKGROUND: ABO subtypes are characterized by the alteration of antigens present and their expression levels on red blood cells and many are linked to genetic changes in the ABO gene. Weakened expression of antigens should be identified to prevent transfusion reactions or ABO-incompatible transplantations. Genotyping can be applied to identify subtypes to complement serologic testing. Next-generation sequencing (NGS) has shown to provide sensitive and accurate genotyping results as well as valuable cis/trans information...
May 16, 2018: Transfusion
https://www.readbyqxmd.com/read/29765587/variation-in-the-degree-of-reciprocal-herkogamy-affects-the-degree-of-legitimate-pollination-in-a-distylous-species
#6
Xian-Feng Jiang, Xing-Fu Zhu, Qing-Jun Li
Distyly is a widespread floral polymorphism characterized by the flowers within a population showing reciprocal placement of the anthers and stigma. Darwin hypothesizes that distyly evolves to promote precise pollen transfer between morphs. Primula chungensis exhibits two types of anther heights, and these two types of anthers show pollen of two different size classes. To understand whether the stigma could capture more pollen grains from the anthers of the pollen donor as the separation between the stigma of pollen receiver and the anther of pollen donor decreased, the present research assessed the source of the pollen load in a series of open-pollinated flowers with continuous variation of style lengths...
June 2018: AoB Plants
https://www.readbyqxmd.com/read/29765294/which-infectious-blood-donors-could-be-identified-by-the-donor-history-questionnaire-comparison-of-blood-donors-infected-with-hiv-or-hcv-with-notified-cases-from-general-population-in-germany
#7
Karina Preußel, Ruth Offergeld
Background: Potential risks for transfusion-transmissible infections are identified by donor history questionnaires (DHQs), and donors with higher risks are deferred from donation. We assessed to which extent the currently used DHQs support the identification of infections among blood donors. Methods: Between 2006 and 2013, we analyzed data from notified HIV and HCV cases in the general population and positive blood donors in Germany. Logistic regressions were used to identify relevant infection risks...
April 2018: Transfusion Medicine and Hemotherapy
https://www.readbyqxmd.com/read/29750504/c1q-mediated-complement-activation-and-c3-opsonization-trigger-recognition-of-stealth-poly-2-methyl-2-oxazoline-coated-silica-nanoparticles-by-human-phagocytes
#8
Regina Tavano, Luca Gabrielli, Elisa Lubian, Chiara Fedeli, Silvia Visentin, Patrizia Polverino de Laureto, Giorgio Arrigoni, Alessandra Geffner-Smith, Fangfang Chen, Dmitri Simberg, Giulia Morgese, Edmondo Maria Benetti, Linping Wu, Seyed Moein Moghimi, Fabrizio Mancin, Emanuele Papini
Poly(2-methyl-2-oxazoline) (PMOXA) is an alternative promising polymer to poly(ethylene glycol), (PEG), for design and engineering of macrophage-evading nanoparticles (NPs). Although PMOXA engineered NPs have shown comparable pharmacokinetics, and in vivo performance to PEGylated stealth NPs in the murine model, its interaction with elements of the human innate immune system has not been studied. From a translational angle, we studied the interaction of fully characterized PMOXA-coated vinyl-triethoxysilane-derived organically modified silica NPs (PMOXA-coated NPs) of approximately 100 nm in diameter with human complement system, blood leukocytes and macrophages, and compared their performance with PEGylated and uncoated NP counterparts...
May 11, 2018: ACS Nano
https://www.readbyqxmd.com/read/29731068/comparison-of-sensitivity-of-immunocomplex-capture-fluorescence-analysis-for-detecting-donor-specific-anti-hla-class-ii-antibodies-in-kidney-transplant-patients
#9
N Ichimaru, T Takayama, H Hirase, Y Hisayama, M Kawamura, S Nakazawa, T Kato, T Abe, J-Y Kaimori, R Imamura, N Nonomura, S Takahara
BACKGROUND: Immunocomplex capture fluorescence analysis (ICFA) detects donor-specific antihuman leukocyte antigen (HLA) antibodies (DSA), but the detection sensitivity of HLA class II antibodies using conventional ICFA is as low as 57%. The aim of the study was to improve the detection sensitivity of HLA class II antibodies by ICFA, and compare the ICFA results with the Luminex single-antigen bead test. METHODS: Six DSA-negative kidney transplant donors and recipient pairs and 10 HLA class II DSA-positive pairs were included in the study...
May 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29721368/cell-surface-vimentin-positive-macrophage-like-circulating-tumor-cells-as-a-novel-biomarker-of-metastatic-gastrointestinal-stromal-tumors
#10
Heming Li, Qing H Meng, Hyangsoon Noh, Neeta Somaiah, Keila E Torres, Xueqing Xia, Izhar S Batth, Cissimol P Joseph, Mengyuan Liu, Ruoyu Wang, Shulin Li
The clinical utility of circulating tumor cells (CTCs) has been investigated in numerous publications, but CTCs that express very typical immune cell markers have not been reported. Here we report a novel class of CTCs-CSV-positive macrophage-like CTCs (ML-CTCs). This nomenclature was based on the fact that this class of CTCs can be captured from blood samples of gastrointestinal stromal tumors (GISTs) patients using either the macrophage marker CD68 or our proprietary tumor-specific cell-surface vimentin (CSV) antibody 84-1; likewise, the captured ML-CTCs can be co-stained with both typical macrophage markers (CD14, CD68) and tumor cell markers (DOG-1, C-kit) but not CD45...
2018: Oncoimmunology
https://www.readbyqxmd.com/read/29714029/a-data-driven-patient-blood-management-strategy-in-liver-transplantation
#11
R A Metcalf, M B Pagano, J R Hess, J Reyes, J D Perkins, M I Montenovo
BACKGROUND AND OBJECTIVES: Blood utilization during liver transplant has decreased, but remains highly variable due to many complex surgical and physiologic factors. Previous models attempted to predict utilization using preoperative variables to stratify cases into two usage groups, usually using entire blood units for measurement. We sought to develop a practical predictive model using specific transfusion volumes (in ml) to develop a data-driven patient blood management strategy. MATERIALS AND METHODS: This is a retrospective evaluation of primary liver transplants at a single institution from 2013 to 2015...
May 1, 2018: Vox Sanguinis
https://www.readbyqxmd.com/read/29694028/noncompetitive-homogeneous-detection-of-small-molecules-using-synthetic-nanopeptamer-based-luminescent-oxygen-channeling
#12
Gabriel Lassabe, Karl Kramer, Bruce D Hammock, Gualberto G Gonzalez-Sapienza, Andrés González-Techera
Our group has previously developed immunoassays for noncompetitive detection of small molecules based on the use of phage borne anti-immunocomplex peptides. Recently, we substituted the phage particles by biotinylated synthetic anti-immunocomplex peptides complexed with streptavidin, and named these constructs nanopeptamers. In this work, we report the results of combining AlphaLisa, a commercial luminescent oxygen channeling bead system, with nanopeptamers for the development of a noncompetitive homogeneous assay for the detection of small molecules...
April 25, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29686997/identification-of-il-28b-genotype-modification-in-hepatocytes-after-living-donor-liver-transplantation-by-laser-capture-microdissection-and-pyrosequencing-analysis
#13
King-Wah Chiu, Toshiaki Nakano, Kuang-Den Chen, Tsung-Hui Hu, Chih-Che Lin, Li-Wen Hsu, Chao-Long Chen, Shigeru Goto
The aim of this study is to elucidate the biogenetic modification of donor and recipient interleukin-28B (IL-28B) genotypes in liver graft biopsies after living donor liver transplantation (LDLT) for chronic hepatitis C virus- (HCV-) related, end-stage liver disease. Fifty liver graft biopsies were collected from recipients during LDLT treatment for HCV-related, end-stage liver disease. DNA was extracted from all 50 liver tissues, and the IL-28B single-nucleotide polymorphisms (SNPs) rs8099917 and rs12979860 were studied for allelic discrimination by real-time PCR analysis...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29684059/supervised-machine-learning-reveals-introgressed-loci-in-the-genomes-of-drosophila-simulans-and-d-sechellia
#14
Daniel R Schrider, Julien Ayroles, Daniel R Matute, Andrew D Kern
Hybridization and gene flow between species appears to be common. Even though it is clear that hybridization is widespread across all surveyed taxonomic groups, the magnitude and consequences of introgression are still largely unknown. Thus it is crucial to develop the statistical machinery required to uncover which genomic regions have recently acquired haplotypes via introgression from a sister population. We developed a novel machine learning framework, called FILET (Finding Introgressed Loci via Extra-Trees) capable of revealing genomic introgression with far greater power than competing methods...
April 23, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29671734/instrumental
#15
Ryoko Hamaguchi
Incorporating classmates' unique one-word reflections on human anatomy and the experience of engaging with the bodies of our anatomical donors, this series captures a diverse spectrum of human emotions surrounding anatomical dissection. Grappling with the humanity of our varied reactions, we related ourselves-ethically and clinically, individually and collectively-to our first "patients" and their critical role in our education and growth as physicians.
April 1, 2018: AMA Journal of Ethics
https://www.readbyqxmd.com/read/29644251/twenty-year-evolution-of-hepatitis-b-virus-and-human-immunodeficiency-virus-prevalence-and-incidence-in-voluntary-blood-donors-in-c%C3%A3-te-d-ivoire
#16
Benjamin Seri, Albert Minga, Delphine Gabillard, Bamori Dembele, Seidou Konate, Jérôme Le Carrou, Lambert Dohoun, Yao Abo, Sophie Karcher, Patrick Coffie, Thérèse N'Dri-Yoman, Alain Attia, Serge P Eholié, Christine Danel, Karine Lacombe, Xavier Anglaret, Anders Boyd
Background: Hepatitis B virus (HBV) and human immunodeficiency virus (HIV) share common risk factors. The parallel description of their frequency over time may help capture their similarities and differences. Methods: Using data from the National Transfusion Center of Abidjan, we estimated the following over a 20-year period: (1) the prevalence of HIV and hepatitis B surface antigen (HBsAg) positivity at first contact; and (2) the incidence of HIV and HBsAg seroconversion in negative first-time blood donors...
April 2018: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/29643468/a-standardized-flow-cytometry-network-study-for-the-assessment-of-circulating-endothelial-cell-physiological-ranges
#17
Paola Lanuti, Pasquale Simeone, Gianluca Rotta, Camillo Almici, Giuseppe Avvisati, Rosa Azzaro, Giuseppina Bologna, Alfredo Budillon, Melania Di Cerbo, Elena Di Gennaro, Maria Luisa Di Martino, Annamaria Diodato, Paolo Doretto, Eva Ercolino, Alessandra Falda, Chiara Gregorj, Alessandra Leone, Francesca Losa, Natalia Malara, Mirella Marini, Pasquale Mastroroberto, Vincenzo Mollace, Michele Morelli, Emma Muggianu, Giuseppe Musolino, Arabella Neva, Laura Pierdomenico, Silvia Pinna, Giovanna Piovani, Maria Serena Roca, Domenico Russo, Lorenza Scotti, Maria Cristina Tirindelli, Valentina Trunzo, Roberta Venturella, Carlo Vitagliano, Fulvio Zullo, Marco Marchisio, Sebastiano Miscia
Circulating endothelial cells (CEC) represent a restricted peripheral blood (PB) cell subpopulation with high potential diagnostic value in many endothelium-involving diseases. However, whereas the interest in CEC studies has grown, the standardization level of their detection has not. Here, we undertook the task to align CEC phenotypes and counts, by standardizing a novel flow cytometry approach, within a network of six laboratories. CEC were identified as alive/nucleated/CD45negative/CD34bright/CD146positive events and enumerated in 269 healthy PB samples...
April 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29643204/l1-retrotransposition-is-a-common-feature-of-mammalian-hepatocarcinogenesis
#18
Stephanie N Schauer, Patricia E Carreira, Ruchi Shukla, Daniel J Gerhardt, Patricia Gerdes, Francisco J Sanchez-Luque, Paola Nicoli, Michaela Kindlova, Serena Ghisletti, Alexandre Dos Santos, Delphine Rapoud, Didier Samuel, Jamila Faivre, Adam D Ewing, Sandra R Richardson, Geoffrey J Faulkner
The retrotransposon Long Interspersed Element 1 (LINE-1 or L1) is a continuing source of germline and somatic mutagenesis in mammals. Deregulated L1 activity is a hallmark of cancer, and L1 mutagenesis has been described in numerous human malignancies. We previously employed retrotransposon capture sequencing (RC-seq) to analyze hepatocellular carcinoma (HCC) samples from patients infected with hepatitis B or hepatitis C virus and identified L1 variants responsible for activating oncogenic pathways. Here, we have applied RC-seq and whole-genome sequencing (WGS) to an Abcb4 (Mdr2) -/- mouse model of hepatic carcinogenesis and demonstrated for the first time that L1 mobilization occurs in murine tumors...
April 11, 2018: Genome Research
https://www.readbyqxmd.com/read/29625577/gene-therapy-in-rare-diseases-the-benefits-and-challenges-of-developing-a-patient-centric-registry-for-strimvelis-in-ada-scid
#19
Heide Stirnadel-Farrant, Mahesh Kudari, Nadia Garman, Jessica Imrie, Bikramjit Chopra, Stefania Giannelli, Michela Gabaldo, Ambra Corti, Stefano Zancan, Alessandro Aiuti, Maria Pia Cicalese, Rohit Batta, Jonathan Appleby, Mario Davinelli, Pauline Ng
BACKGROUND: Strimvelis (autologous CD34+ cells transduced to express adenosine deaminase [ADA]) is the first ex vivo stem cell gene therapy approved by the European Medicines Agency (EMA), indicated as a single treatment for patients with ADA-severe combined immunodeficiency (ADA-SCID) who lack a suitable matched related bone marrow donor. Existing primary immunodeficiency registries are tailored to transplantation outcomes and do not capture the breadth of safety and efficacy endpoints required by the EMA for the long-term monitoring of gene therapies...
April 6, 2018: Orphanet Journal of Rare Diseases
https://www.readbyqxmd.com/read/29620284/enrichment-and-mutation-detection-of-circulating-tumor-cells-from-blood-samples
#20
Ruqin Kou, Jian Zhao, Priya Gogoi, Shannon Carskadon, Will Chow, Clara Hwang, Nallasivam Palanisamy, Conrad Leung, Yixin Wang
The potential of circulating tumor cells (CTCs) in the diagnosis and prognosis of cancer patients has become increasingly attractive. However, molecular analysis of CTCs is hindered by low sensitivity and a high level of background leukocytes in CTC enrichment technologies. We have developed a novel protocol using a microfluidic device, which enriches and retrieves CTCs from blood samples. The principle of CTC capturing is that tumor cells are larger and less deformable than normal blood cells. To evaluate the potential of utilizing Celsee PREP100 in CTC molecular analysis, we prepared prostate cancer cell lines PC3 and LNCaP, retrieved the captured cells and analyzed them using PCR amplicon sequencing...
March 30, 2018: Oncology Reports
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