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https://www.readbyqxmd.com/read/28396528/human-enterocytes-as-an-in-vitro-model-for-the-evaluation-of-intestinal-drug-metabolism-characterization-of-drug-metabolizing-enzyme-activities-of-cryopreserved-human-enterocytes-from-twenty-four-donors
#1
Ming-Chih David Ho, Nick Ring, Kirsten Amaral, Utkarsh Doshi, Albert Li
We report here successful isolation and cryopreservation of enterocytes from human small intestine. The enterocytes were isolated by enzyme digestion of the intestinal lumen followed by partial purification via differential centrifugation. The enterocytes were cryopreserved directly after isolation without culturing to maximize retention of in vivo drug metabolizing enzyme activities. Post-thaw viability of the cryopreserved enterocytes was consistently over 80% based on trypan blue exclusion. Cryopreserved enterocytes pooled from 8 donors (4 male and 4 female) were evaluated for their metabolism of 14 pathway-selective substrates: CYP1A2 (phenacetin hydroxylation), CYP2A6 (coumarin 7-hydroxylation), CYP2B6 (bupropion hydroxylation), CYP2C8 (paclitaxel 6α-hydroxylation), CYP2C9 (diclofenac 4-hydroxylation), CYP2C19 (s-mephenytoin 4-hydroxylation), CYP2D6 (dextromethorphan hydroxylation), CYP2E1 (chlorzoxazone 6-hydroxylation), CYP3A4 (midazolam 1'-hydroxylation and testosterone 6β-hydroxylation), CYP2J2 (astemizole O-demethylation), UDP-glucuronosyltransferase (UGT; 7-hydroxycoumarin glucuronidation), sulfotransferase (SULT; 7-hydroxycoumarin sulfation), and carboxylesterase 2 (CES2; irinotecan hydrolysis) activities...
April 10, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/28394128/water-soluble-pd8l4-self-assembled-molecular-barrel-as-an-aqueous-carrier-for-hydrophobic-curcumin
#2
Imtiyaz Ahmad Bhat, Ruchi Jain, Mujahuddin M Siddiqui, Deepak K Saini, Partha Sarathi Mukherjee
A tetrafacial water-soluble molecular barrel (1) was synthesized by coordination driven self-assembly of a symmetrical tetrapyridyl donor (L) with a cis-blocked 90° acceptor [cis-(en)Pd(NO3)2] (en = ethane-1,2-diamine). The open barrel structure of (1) was confirmed by single crystal X-ray diffraction. The presence of a hydrophobic cavity with large windows makes it an ideal candidate for encapsulation and carrying hydrophobic drug like curcumin in an aqueous medium. The barrel (1) encapsulates curcumin inside its molecular cavity and protects highly photosensitive curcumin from photodegradation...
April 10, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28394024/a-brief-motivational-interview-promotes-internal-motivation-to-donate-blood-among-young-adults-with-and-without-a-prior-donation-history
#3
Irina E Livitz, Kristen R Fox, Lina K Himawan, Christopher R France
BACKGROUND: Recruitment and retention of first-time and repeat donors is essential to maintain a stable blood supply. Recent evidence has shown that promoting internal motivation may be an effective strategy to enhance donation behavior. We tested the efficacy of an in-person motivational interview at increasing internal motivation and intention to donate. STUDY DESIGN AND METHODS: A sample of 219 donors and nondonors (69.4% female; mean ± SD age, 19.2 ± 1...
April 10, 2017: Transfusion
https://www.readbyqxmd.com/read/28389623/neoplastic-plasma-cells-generate-an-inflammatory-environment-within-bone-marrow-and-markedly-alter-the-distribution-of-t-cells-between-lymphoid-compartments
#4
Oliver C Goodyear, Sarah Essex, Anandram Seetharam, Supratik Basu, Paul Moss, Guy Pratt
Monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM) are characterised by the accumulation of malignant plasma cells within bone marrow and lead to a range of abnormalities in the peripheral blood T cell repertoire. We investigated the level of inflammatory chemokines within the bone marrow and blood of patients with MGUS and MM and related this to the pattern of chemokine receptor expression on T cells in both compartments.The expression of a wide range of chemokine ligands for CXCR3 and CCR4 was markedly increased within the bone marrow of patients with MGUS and MM compared to healthy donors...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28389600/intravascular-donor-monocytes-play-a-central-role-in-lung-transplant-ischaemia-reperfusion-injury
#5
Kate Colette Tatham, Kieran Patrick O'Dea, Rosalba Romano, Hannah Elizabeth Donaldson, Kenji Wakabayashi, Brijesh Vipin Patel, Louit Thakuria, Andre Rudiger Simon, Padmini Sarathchandra, Nandor Marczin, Masao Takata
RATIONALE: Primary graft dysfunction in lung transplant recipients derives from the initial, largely leukocyte-dependent, ischaemia-reperfusion injury. Intravascular lung-marginated monocytes have been shown to play key roles in experimental acute lung injury, but their contribution to lung ischaemia-reperfusion injury post transplantation is unknown. OBJECTIVE: To define the role of donor intravascular monocytes in lung transplant-related acute lung injury and primary graft dysfunction...
April 7, 2017: Thorax
https://www.readbyqxmd.com/read/28381264/hiv-policy-implementation-in-two-health-and-demographic-surveillance-sites-in-uganda-findings-from-a-national-policy-review-health-facility-surveys-and-key-informant-interviews
#6
Ellen McRobie, Alison Wringe, Jessica Nakiyingi-Miiro, Francis Kiweewa, Tom Lutalo, Gertrude Nakigozi, Jim Todd, Jeffrey William Eaton, Basia Zaba, Kathryn Church
BACKGROUND: Successful HIV testing, care and treatment policy implementation is essential for realising the reductions in morbidity and mortality those policies are designed to target. While adoption of new HIV policies is rapid, less is known about the facility-level implementation of new policies and the factors influencing this. METHODS: We assessed implementation of national policies about HIV testing, treatment and retention at health facilities serving two health and demographic surveillance sites (HDSS) (10 in Kyamulibwa, 14 in Rakai)...
April 5, 2017: Implementation Science: IS
https://www.readbyqxmd.com/read/28361127/longterm-maintenance-of-human-naive-t-cells-through-in-situ-homeostasis-in-lymphoid-tissue-sites
#7
Joseph J C Thome, Boris Grinshpun, Brahma V Kumar, Masa Kubota, Yoshiaki Ohmura, Harvey Lerner, Gregory D Sempowski, Yufeng Shen, Donna L Farber
Naïve T cells develop in the thymus and coordinate immune responses to new antigens; however, mechanisms for their long-term persistence over the human lifespan remain undefined. Here, we investigated human naïve T cell development and maintenance in primary and secondary lymphoid tissues obtained from individual organ donors aged 3 months-73 years. In the thymus, the frequency of double-positive thymocytes declined sharply in donors over age 40 coincident with reduced recent thymic emigrants (RTE) in lymphoid tissues, while naïve T cells were functionally maintained predominantly in lymph nodes (LN)...
December 2016: Science Immunology
https://www.readbyqxmd.com/read/28360110/the-cftr-trafficking-mutation-f508del-inhibits-the-constitutive-activity-of-slc26a9
#8
Carol A Bertrand, Shalini Mitra, Sanjay K Mishra, Xiaohui Wang, Yu Zhao, Joseph M Pilewski, Dean R Madden, Raymond A Frizzell
Several members of the SLC26A family of anion transporters associate with CFTR, forming complexes in which CFTR and SLC26A functions are reciprocally regulated. This association is thought to be facilitated by PDZ scaffolding interactions. CFTR has been shown to be positively regulated by NHERF-1, and negatively regulated by CAL in airway epithelia. However, it's unclear which PDZ-domain protein(s) interact with SLC26A9, a SLC26A family member found in airway epithelia. We have previously shown that primary, human bronchial epithelia (HBE) from non-CF donors exhibit constitutive anion secretion attributable to SLC26A9...
March 30, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28336244/-commitment-in-plasmapheresis-donation-a-study-of-determinants-among-regular-donors-in-plasmapheresis
#9
S Delépine-Farvacques, Florence Terrade, B Danic, J-P Lebaudy
The purpose of this study, based on the Theory of planned behavior, is to identify and ultimately better understand what determines the fidelity and the regularity of donors committed in plasmapheresis donation. This qualitative study is a primary stage to establish a classification of donors in a French socio-cultural context. For this reason, we have carried out a survey among 16 regular plasmapheresis donors, by way of semi-structured individual interviews at the Établissement français du sang - Brittany...
March 20, 2017: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
https://www.readbyqxmd.com/read/28324826/nitrogen-removal-by-the-enhanced-floating-treatment-wetlands-from-the-secondary-effluent
#10
Lei Gao, Weili Zhou, Jungchen Huang, Shengbing He, Yijia Yan, Wenying Zhu, Suqing Wu, Xu Zhang
Three novel floating treatment wetlands, including autotrophic enhanced floating treatment wetland (AEFTW), heterotrophic enhanced floating treatment wetland (HEFTW) and enhanced floating treatment wetland (EFTW) were developed to remove nitrogen from the secondary effluent. Results showed that the analogously excellent nitrogen removal performance was achieved in AEFTW and HEFTW. About 89.4% of the total nitrogen (TN) was removed from AEFTW at a low S/N of 0.9 and 88.5% from HEFTW at a low C/N of 3.5 when the hydraulic retention time (HRT) was 1d in summer...
March 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28319197/the-hiv-care-cascade-japanese-perspectives
#11
Aikichi Iwamoto, Rikizo Taira, Yoshiyuki Yokomaku, Tomohiko Koibuchi, Mahbubur Rahman, Yoko Izumi, Kenji Tadokoro
Japan has been known as a low HIV-prevalence country with a concentrated epidemic among high-risk groups. However, it has not been determined whether Japan meets the 90-90-90 goals set by the Joint United Nations Programme on HIV/AIDS (UNAIDS)/World Health Organization (WHO). Moreover, to date, the HIV care cascade has not been examined. We estimated the total number of diagnosed people living with HIV/AIDS (PLWHA) (n = 22,840) based on legal reports to the Ministry of Health, Labour and Welfare by subtracting the number of foreigners who left Japan (n = 2,273) and deaths (n = 2,321) from the cumulative diagnosis report (n = 27,434)...
2017: PloS One
https://www.readbyqxmd.com/read/28297575/selective-inhibition-of-adam17-efficiently-mediates-glycoprotein-ib%C3%AE-retention-during-ex-vivo-generation-of-human-induced-pluripotent-stem-cell-derived-platelets
#12
Shinji Hirata, Takahiko Murata, Daisuke Suzuki, Sou Nakamura, Ryoko Jono-Ohnishi, Hidenori Hirose, Akira Sawaguchi, Satoshi Nishimura, Naoshi Sugimoto, Koji Eto
Donor-independent platelet concentrates for transfusion can be produced in vitro from induced pluripotent stem cells (iPSCs). However, culture at 37°C induces ectodomain shedding on platelets of glycoprotein Ibα (GPIbα), the von Willebrand factor receptor critical for adhesive function and platelet lifetime in vivo, through temperature-dependent activation of a disintegrin and metalloproteinase 17 (ADAM17). The shedding can be suppressed by using inhibitors of panmetalloproteinases and possibly of the upstream regulator p38 mitogen-activated protein kinase (p38 MAPK), but residues of these inhibitors in the final platelet products may be accompanied by harmful risks that prevent clinical application...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28284766/multi-mode-application-of-graphene-quantum-dots-bonded-silica-stationary-phase-for-high-performance-liquid-chromatography
#13
Qi Wu, Yaming Sun, Xiaoli Zhang, Xia Zhang, Shuqing Dong, Hongdeng Qiu, Litao Wang, Liang Zhao
Graphene quantum dots (GQDs), which possess hydrophobic, hydrophilic, π-π stacking and hydrogen bonding properties, have great prospect in HPLC. In this study, a novel GQDs bonded silica stationary phase was prepared and applied in multiple separation modes including normal phase, reversed phase and hydrophilic chromatography mode. Alkaloids, nucleosides and nucleobases were chosen as test compounds to evaluate the separation performance of this column in hydrophilic chromatographic mode. The tested polar compounds achieved baseline separation and the resolutions reached 2...
February 28, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28265591/tubulovillous-adenoma-in-the-bladder-in-a-dual-pancreas-kidney-transplant-patient
#14
Taylor Remondini, Stephan Van Zyl, Tarek A Bismar, Serdar Yilmaz, M Eric Hyndman
Background: A rare report of a tubulovillous adenoma arising in the setting of a dual pancreas-kidney transplant patient. Case Presentation: This adenoma was discovered in a 60-year-old male with a dual pancreas-kidney transplant that presented with urinary retention and gross hematuria. Management of this patient required both transurethral resection of the tumor as well as a laparotomy after recurrence. Follow-up with cystoscopy has shown no further recurrence of the tumor. Conclusion: This case adds to the few cases documented of adenomas arising in bladders augmented with gastrointestinal tract tissue...
2017: Journal of Endourology Case Reports
https://www.readbyqxmd.com/read/28258369/the-alternative-splicing-of-eam8-contributes-to-early-flowering-and-short-season-adaptation-in-a-landrace-barley-from-the-qinghai-tibetan-plateau
#15
Tengfei Xia, Lianquan Zhang, Jinqing Xu, Lei Wang, Baolong Liu, Ming Hao, Xi Chang, Tangwei Zhang, Shiming Li, Huaigang Zhang, Dengcai Liu, Yuhu Shen
The early flowering of Lalu was determined to be due to a novel spontaneous eam8 mutation, which resulted in intron retention and the formation of a putative truncated protein. Barley is a staple crop grown over an extensive area in the Qinghai-Tibetan Plateau. Understanding the genetic mechanism for its success in a high altitude is important for crop improvement in marginal environments. Early flowering is a critical adaptive trait that strongly influences reproductive fitness in a short growing season. Loss-of-function mutations at the circadian clock gene EARLY MATURITY 8 (EAM8) promote rapid flowering...
April 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28250536/adapting-nepal-s-polio-eradication-programme
#16
Krishna P Paudel, Lee M Hampton, Santosh Gurung, Rajendra Bohara, Indra K Rai, Sameer Anaokar, Rachel D Swift, Stephen Cochi
PROBLEM: Many countries have weak disease surveillance and immunization systems. The elimination of polio creates an opportunity to use staff and assets from the polio eradication programme to control other vaccine-preventable diseases and improve disease surveillance and immunization systems. APPROACH: In 2003, the active surveillance system of Nepal's polio eradication programme began to report on measles and neonatal tetanus cases. Japanese encephalitis and rubella cases were added to the surveillance system in 2004...
March 1, 2017: Bulletin of the World Health Organization
https://www.readbyqxmd.com/read/28241882/systematic-review-of-patient-factors-affecting-adipose-stem-cell-viability-and-function-implications-for-regenerative-therapy
#17
REVIEW
Jajini Varghese, Michelle Griffin, Afshin Mosahebi, Peter Butler
BACKGROUND: The applications for fat grafting have increased recently, within both regenerative and reconstructive surgery. Although fat harvesting, processing and injection techniques have been extensively studied and standardised, this has not had a big impact on the variability of outcome following fat grafting. This suggests a possible larger role of patient characteristics on adipocyte and adipose-derived stem cell (ADSC) viability and function. This systematic review aims to collate current evidence on the effect of patient factors on adipocyte and ADSC behaviour...
February 28, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28241418/the-effect-of-simulated-flash-heat-pasteurization-on-immune-components-of-human-milk
#18
Brodie Daniels, Stefan Schmidt, Tracy King, Kiersten Israel-Ballard, Kimberly Amundson Mansen, Anna Coutsoudis
A pasteurization temperature monitoring system has been designed using FoneAstra, a cellphone-based networked sensing system, to monitor simulated flash-heat (FH) pasteurization. This study compared the effect of the FoneAstra FH (F-FH) method with the Sterifeed Holder method currently used by human milk banks on human milk immune components (immunoglobulin A (IgA), lactoferrin activity, lysozyme activity, interleukin (IL)-8 and IL-10). Donor milk samples (N = 50) were obtained from a human milk bank, and pasteurized...
February 22, 2017: Nutrients
https://www.readbyqxmd.com/read/28217338/5-amino-1-2-3-o-iso-propyl-idene-d-ribit-yl-1h-imidazole-4-carboxamide-a-crystal-structure-with-z-4
#19
Vincenzo Piccialli, Nicola Borbone, Giorgia Oliviero, Gennaro Piccialli, Stefano D'Errico, Roberto Centore
The title compound, C12H20N4O5, crystallizes in the monoclinic space group P21, with four crystallographically independent mol-ecules in the asymmetric unit. The four mol-ecules have a very similar conformation that is basically determined by the formation of two intra-molecular hydrogen bonds between the amino NH2 donors and the carbonyl and ring O-atom acceptors, forming, respectively, R(6) and R(7) ring motifs.. In the crystal, inter-molecular hydrogen bonding leads to the formation of R2(2)(10) ring patterns, involving one amide CONH2 donor and an imidazole N-atom acceptor...
February 1, 2017: Acta Crystallographica. Section E, Crystallographic Communications
https://www.readbyqxmd.com/read/28208057/vertical-alveolar-distraction-osteogenesis-of-the-atrophic-posterior-mandible-before-dental-implant-insertion
#20
Adi Rachmiel, Dekel Shilo, Dror Aizenbud, Omri Emodi
PURPOSE: Augmentation of deficient mandibular posterior alveolar ridges poses a great challenge because of extensive bone deficiency and the presence of the inferior alveolar nerve. This study sets its focus on vertical alveolar distraction osteogenesis (ADO) at the posterior mandible before dental implant placement. MATERIALS AND METHODS: The study included 21 sites in 18 patients with severe mandibular posterior alveolar ridge deficiency. A trapezoidal osteotomy was performed, and an extraosseous alveolar distraction device was fixed and activated after a 4-day latency period at a rate of 0...
January 21, 2017: Journal of Oral and Maxillofacial Surgery
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