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https://www.readbyqxmd.com/read/28646801/the-effects-of-exposure-to-low-frequency-electromagnetic-fields-on-male-fertility
#1
Mahsa Darbandi, Sara Darbandi, Ashok Agarwal, Ralf Henkle, Mohammad Reza Sadeghi
Context • People are increasingly exposed to low frequency (LF) electromagnetic fields (EMFs), mainly from electricity distribution networks and electronic devices. Critics of this widespread exposure believe that it can have detrimental effects on the human body. On the other hand, many in vivo and in vitro studies have claimed that low frequency electromagnetic therapy can function as a form of alternative medicine and that therapists can treat disease by applying electromagnetic radiation or pulsed EMFs to the body or cells...
June 23, 2017: Alternative Therapies in Health and Medicine
https://www.readbyqxmd.com/read/28595604/functionality-and-acceptability-of-a-wireless-fetal-heart-rate-monitoring-device-in-term-pregnant-women-in-rural-southwestern-uganda
#2
Godfrey R Mugyenyi, Esther C Atukunda, Joseph Ngonzi, Adeline Boatin, Blair J Wylie, Jessica E Haberer
BACKGROUND: Over 3 million stillbirths occur annually in sub Saharan Africa; most occur intrapartum and are largely preventable. The standard of care for fetal heart rate (FHR) assessment in most sub-Saharan African settings is a Pinard Stethoscope, limiting observation to one person, at one point in time. We aimed to test the functionality and acceptability of a wireless FHR monitor that could allow for expanded monitoring capacity in rural Southwestern Uganda. METHODS: In a mixed method prospective study, we enrolled 1) non-laboring healthy term pregnant women to wear the device for 30 min and 2) non-study clinicians to observe its use...
June 8, 2017: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/28591488/salvage-of-fetal-karyotype-information-from-snp-array-data-obtained-from-products-of-conception-with-maternal-cell-contamination
#3
Karin Sasaki, Kosei Abe, Takahide Mori, Kazunori Hashimoto, Kazuhiko Nakabayashi
OBJECTIVE: Maternal cell contamination (MCC) is known to increase the risk of misdiagnosis in prenatal diagnosis as well as in diagnostic tests for the products of conception (POC) from miscarriages. We aimed to develop a data correction method to salvage fetal karyotype information from SNP array data for POC with MCC when parental genotype data are available. METHODS: We obtained SNP array data from mixed genomic DNAs of a mother-child pair and used the dataset to assess the accuracy of data correction...
June 7, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/28553771/the-first-trimester-combined-test-for-aneuploidies-a-single-center-experience
#4
Alina Veduta, Ana Maria Vayna, Simona Duta, Anca Panaitescu, Florin Popescu, Maria Bari, Gheorghe Peltecu, Florina Nedelea
PURPOSE: We present the results of the systematic application of the first trimester combined test for aneuploidies, in a Romanian center. METHODS: Since October 2009, in Filantropia Hospital in Bucharest, we have systematically been using the FMF (Fetal Medicine Foundation) combined first trimester test to screen for common aneuploidies at 11 to 13 + 6 weeks of gestation. We assessed the crown - rump length (CRL), nuchal translucency, fetal heart rate as well as PAPP-A and free β-hCG in maternal serum...
May 28, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28544538/cornelia-de-lange-syndrome-to-diagnose-or-not-to-diagnose-in-utero
#5
REVIEW
Laura Avagliano, Gaetano Pietro Bulfamante, Valentina Massa
Cornelia de Lange syndrome (CdLS) is an inherited condition with a wide spectrum of phenotypic anomalies, consisting mainly of growth impairment, multi-organ abnormalities, and neurocognitive delay. Clinical diagnostic criteria after birth are well defined, whereas when to suspect the syndrome during intrauterine life still remains undefined. This review summarizes the main possible prenatal findings in CdLS, suggesting that a skilled ultrasound scan in cases of intrauterine growth restriction associated with other fetal abnormalities may improve the chance of prenatal diagnosis of CdLS, especially in families known to be at high risk...
May 22, 2017: Birth defects research
https://www.readbyqxmd.com/read/28539859/human-induced-pluripotent-stem-cells-generated-neural-cells-behaving-like-brain-and-spinal-cord-cells-an-insight-into-the-involvement-of-retinoic-acid-and-sonic-hedgehog-proteins
#6
Akinlolu Abdulazeez Adelaja
OBJECTIVES: The previous studies generated neural progenitor cells (NPCs) from human induced pluripotent stem cells (hiPSCs) using different protocols. However, the nature of the temporal or regional specificity of NPCs derived using these protocols is not well defined. Therefore, this study aimed to generate age- and region-specific NPCs from hiPSCs, which mimic in vivo fetal brain (FNPC-B) or spinal cord (FNPC-SC) tissues, in the absence or presence of retinoic acid (RA) and sonic hedgehog (SHH)...
April 2017: International Journal of Health Sciences
https://www.readbyqxmd.com/read/28539259/congenital-anomalies-in-tunisia-frequency-and-risk-factors
#7
M Aloui, K Nasri, A M Ben Hamida, R Marrakchi, S S Gaïgi
BACKGROUND: Despite the high risk of recurrence of congenital malformations, there are no well-accepted preventive measures in developing countries like Tunisia. It is recommended that thorough epidemiological studies of congenital anomalies in this country are needed. The aim of this study is to assess the frequency and types of congenital anomalies in Tunisia and research some risk factors associated with occurrence of these anomalies. METHODS: In this retrospective study, all the fetuses who were autopsied during 21years period from February 1991 to December 2011 (n=9678) at Service of embryofetopathology in the Center of Maternity and Neonatology of Tunis (CMNT) were studied...
May 21, 2017: Journal of gynecology obstetrics and human reproduction
https://www.readbyqxmd.com/read/28536927/involvement-of-the-dna-mismatch-repair-system-in-cisplatin-sensitivity-of-testicular-germ-cell-tumours
#8
Christiane Rudolph, Cecilie Melau, John E Nielsen, Kristina Vile Jensen, Dekang Liu, Javier Pena-Diaz, Ewa Rajpert-De Meyts, Lene Juel Rasmussen, Anne Jørgensen
BACKGROUND: Testicular germ cell tumours (TGCT) are highly sensitive to cisplatin-based chemotherapy, but patients with tumours containing differentiated teratoma components are less responsive to this treatment. The cisplatin sensitivity in TGCT has previously been linked to the embryonic phenotype in the majority of tumours, although the underlying mechanism largely remains to be elucidated. The aim of this study was to investigate the role of the DNA mismatch repair (MMR) system in the cisplatin sensitivity of TGCT...
May 23, 2017: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/28535307/intergenerational-impact-of-paternal-lifetime-exposures-to-both-folic-acid-deficiency-and-supplementation-on-reproductive-outcomes-and-imprinted-gene-methylation
#9
Lundi Ly, Donovan Chan, Mahmoud Aarabi, Mylène Landry, Nathalie A Behan, Amanda J MacFarlane, Jacquetta Trasler
STUDY QUESTION: Do paternal exposures to folic acid deficient (FD), and/or folic acid supplemented (FS) diets, throughout germ cell development adversely affect male germ cells and consequently offspring health outcomes? SUMMARY ANSWER: Male mice exposed over their lifetimes to both FD and FS diets showed decreased sperm counts and altered imprinted gene methylation with evidence of transmission of adverse effects to the offspring, including increased postnatal-preweaning mortality and variability in imprinted gene methylation...
May 23, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28492745/cytotoxicity-evaluation-of-root-canal-sealers-using-an-in-vitro-experimental-model-with-roots
#10
Ligia Teixeira, Fernanda Gonçalves Basso, Josimeri Hebling, Carlos Alberto de Souza Costa, Graziela Garrido Mori, Yara Teresinha Corrêa Silva-Sousa, Camila Fávero de Oliveira
The aim of the present study was to evaluate the cytotoxicity of root canal sealers under conditions closely resembling a clinical reality. A primary human fibroblast cell line was seeded in 24-well acrylic plates with Dulbecco's modified Eagle's medium supplemented with 10% serum fetal bovine (SFB) and incubated for 24 h. Root canals from premolars were filled and individually attached to nylon devices to be stabilized in the wells with the already seeded cells. Specimens were divided into groups as follows: Control: gutta-percha cones (GPC); AH Plus+GPC; Sealapex+GPC; MTA Fillapex+GPC and Endofill+GPC...
March 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/28478816/effects-of-hypnosis-during-pregnancy-a-psychophysiological-study-on-maternal-stress
#11
Fabien Legrand, Corinne Grévin-Laroche, Elisabeth Josse, Guillaume Polidori, Hervé Quinart, Redha Taïar
Because it induces a state of reduced awareness and deep relaxation, hypnosis is thought to be efficient at relieving stress and anxiety. This study examined whether hypnosis may alter the pattern and time evolution of maternal and fetal stress. Here we report a 23-yrs-old primigravida woman at 31-weeks' gestation who underwent daily sessions of hypnosis during one week. An A (baseline)-B (intervention) - A' (return to baseline) design was used. Each study phase lasted one week. The State Anxiety Inventory (SAI) was completed daily...
May 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28475393/fetal-scalp-stimulation-fss-versus-fetal-blood-sampling-fbs-for-women-with-abnormal-fetal-heart-rate-monitoring-in-labor-a-prospective-cohort-study
#12
U Tahir Mahmood, C O'Gorman, Z Marchocki, Y M O'Brien, D J Murphy
OBJECTIVE: To evaluate the performance of fetal scalp stimulation (FSS) compared to fetal blood sampling (FBS) as a second line test of fetal wellbeing in labor. STUDY DESIGN: A prospective cohort study was conducted including 298 fetal blood sampling procedures performed due to abnormal fetal cardiotocography (CTG). Two independent observers interpreted the CTG following stimulation. The FSS test was classified as normal when an elicited acceleration and/or provoked fetal heart rate variability was recorded...
May 5, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28472705/measurements-of-red-cell-deformability-and-hydration-reflect-hbf-and-hba2-in-blood-from-patients-with-sickle-cell-anemia
#13
Nermi L Parrow, Hongbin Tu, James Nichols, Pierre-Christian Violet, Corinne A Pittman, Courtney Fitzhugh, Robert E Fleming, Narla Mohandas, John F Tisdale, Mark Levine
Decreased erythrocyte deformability, as measured by ektacytometry, may be associated with disease severity in sickle cell anemia (SCA). Heterogeneous populations of rigid and deformable cells in SCA blood result in distortions of diffraction pattern measurements that correlate with the concentration of hemoglobin S (HbS) and the percentage of irreversibly sickled cells. We hypothesize that red cell heterogeneity, as well as deformability, will also be influenced by the concentration of alternative hemoglobins such as fetal hemoglobin (HbF) and the adult variant, HbA2...
June 2017: Blood Cells, Molecules & Diseases
https://www.readbyqxmd.com/read/28472297/sexual-transmission-of-zikv-causes-fetal-growth-restriction-in-mice
#14
Ryuta Uraki, Kellie Ann Jurado, Jesse Hwang, Klara Szigeti-Buck, Tamas Horvath, Akiko Iwasaki, Erol Fikrig
Zika virus (ZIKV) can be transmitted via mosquito bite or sexual contact. Using mice that lack the type I interferon receptor we examine sexual transmission of ZIKV. Electron microscopy analyses showed association of virions with developing sperm within testes as well as with mature sperm within epididymis. When ZIKV-infected male mice were mated with naïve female mice, the weight of fetuses at embryonic day 18.5 (E18.5) was significantly reduced compared with the control group. Additionally, we found ocular deformities in a minority of the fetuses...
May 2, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28463580/tissue-specific-extracellular-matrix-enhances-skeletal-muscle-precursor-cell-expansion-and-differentiation-for-potential-application-in-cell-therapy
#15
Deying Zhang, Yong Zhang, Yuanyuan Zhang, Hualin Yi, Zhan Wang, Rongpei Wu, Dawei He, Guanghui Wei, Shicheng Wei, Yun Hu, Junhong Deng, Tracy Criswell, James J Yoo, Yu Zhou, Anthony Atala
Skeletal muscle precursor cells (MPCs) are considered a key candidate for cell therapy in the treatment of skeletal muscle dysfunction due to injury, disease or age. However, expansion of a sufficient number of functional skeletal muscle cells in vitro from a small tissue biopsy has been challenging due to changes in phenotypic expression of these cells under traditional culture conditions. Thus, the aim of the study was to develop a better culture system for the expansion and myo-differentiation of MPCs that could further be used for therapy...
May 2, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28437855/mild-fetal-cerebral-ventriculomegaly-prevalence-characteristics-and-utility-of-ancillary-testing-in-cases-presenting-to-a-tertiary-referral-center
#16
Alison J Mehlhorn, Cara E Morin, Jade J Wong-You-Cheong, Stephen A Contag
OBJECTIVE: Ventriculomegaly is the most common fetal brain anomaly identified during prenatal anatomy ultrasound. The aim of our study was to characterize cases of mild ventriculomegaly and investigate the utility of ancillary tests. METHOD: We reviewed 121 cases of mild ventriculomegaly, defined as lateral ventricle diameter of 10-15 mm. Characteristics of the ventricular dilation as well as each pregnancy were investigated. Ancillary tests performed included follow-up MRI, chromosomal abnormality testing, and maternal serologic infection screening...
April 24, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/28430034/um-chor1-establishment-and-characterization-of-the-first-validated-clival-chordoma-cell-line
#17
John Henry Owen, Christine M Komarck, Anthony C Wang, Waleed M Abuzeid, Richard F Keep, Erin L McKean, Stephen Sullivan, Xing Fan, Mark E P Prince
OBJECTIVE Chordomas are rare malignant tumors thought to arise from remnants of the notochord. They can be located anywhere along the axial skeleton but are most commonly found in the clival and sacrococcygeal regions, where the notochord regresses during fetal development. Chordomas are resistant to many current therapies, leaving surgery as the primary method of treatment. Cancer cell lines have been useful for developing new cancer treatments in a laboratory setting that can then be transferred to the clinic, but there are only 4 validated chordoma cell lines available...
April 21, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/28428049/stem-cells-for-retinal-disease-a-perspective-on-the-promise-and-perils
#18
Rajesh C Rao, Vaidehi S Dedania, Mark W Johnson
PURPOSE: To summarize key concepts, and early safety and efficacy signals from clinical trials for stem/progenitor cell-based interventions for retinal disease. DESIGN: Interpretive essay. METHODS: Review and synthesis of selected recent reports of stem/progenitor cell-based approaches for retinal disease, with interpretation and perspective. RESULTS: Stem/progenitor cell-based interventions represent a novel class of potential therapies for retinal diseases, such as age-related macular degeneration, inherited retinal dystrophies, and others...
April 17, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/28412995/development-and-validation-of-prediction-models-for-risks-of-complications-in-early-onset-pre-eclampsia-prep-a-prospective-cohort-study
#19
Shakila Thangaratinam, John Allotey, Nadine Marlin, Ben W Mol, Peter Von Dadelszen, Wessel Ganzevoort, Joost Akkermans, Asif Ahmed, Jane Daniels, Jon Deeks, Khaled Ismail, Ann Marie Barnard, Julie Dodds, Sally Kerry, Carl Moons, Richard D Riley, Khalid S Khan
BACKGROUND: The prognosis of early-onset pre-eclampsia (before 34 weeks' gestation) is variable. Accurate prediction of complications is required to plan appropriate management in high-risk women. OBJECTIVE: To develop and validate prediction models for outcomes in early-onset pre-eclampsia. DESIGN: Prospective cohort for model development, with validation in two external data sets. SETTING: Model development: 53 obstetric units in the UK...
April 2017: Health Technology Assessment: HTA
https://www.readbyqxmd.com/read/28412674/analysis-of-relevant-risk-factors-for-intrauterine-death-of-fetuses-in-the-third-trimester-of-pregnancy
#20
Yang Qiong, Yuan Yichong
Our study aimed to explore the relevant risk factors for intrauterine death of fetuses in the third trimester of pregnancy via a retrospective analysis. Then, 98 pregnant women with intrauterine death of fetuses in the third trimester of pregnancy were enrolled, who had undergone the induced labor of dead fetuses in our hospital from January, 2013 to January, 2015. By taking their disease conditions into considerations, methods of induced labor as softening of cervix with dinoprostone suppositories and amniotic infusion of ethacridine or oxytocin were performed, and the timely cesarean section for termination of pregnancy was performed...
November 2016: Pakistan Journal of Pharmaceutical Sciences
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