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https://www.readbyqxmd.com/read/28823122/genetic-analysis-of-milk-production-traits-of-tunisian-holsteins-using-random-regression-test-day-model-with-legendre-polynomials
#1
Hafedh Ben Zaabza, Abderrahmen Ben Gara, Boulbaba Rekik
Objective: The objective of this study was to estimate genetic parameters of milk, fat, and protein yields within and across lactations in Tunisian Holsteins using a random regression test-day model. Methods: A random regression multiple trait multiple lactation test-day (TD) model was used to estimate genetic parameters in the Tunisian dairy cattle population. Data were TD yields of milk, fat, and protein from the first three lactations. Random regressions were modeled with third-order Legendre polynomials for the additive genetic, and permanent environment effects...
August 16, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28823096/sub-chronic-exposure-to-low-concentration-of-dibutyl-phthalate-affects-anthropometric-parameters-and-markers-of-obesity-in-rats
#2
Khalid Abdul Mjaeed, Habib Ur Rehman, Muhammad Shahbaz Yousaf, Hafsa Zaneb, Imtiaz Rabbani, Sajid Khan Tahir, Muhammad Afzal Rashid
Dibutyl phthalate is an important phthalate ester extensively used in various products like plastics, adhesives, inks, pharmaceuticals, lacquers, varnishes, paper coatings, safety glasses, and cosmetics. The exposure of DBP to "one's health" is therefore inevitable. The present study focuses on elucidating the effect of low doses of DBP on anthropometric parameters and markers of obesity in rats in a 13-week study. A total of 48 rats were divided into three treatment groups as mg DBP/kg body weight per day: (a) 0 mg/kg (control), (b) 10 mg/kg (DBP-10), and (c) 50 mg/kg (DBP-50)...
August 19, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28823069/current-status-of-cattle-production-system-in-nyagatare-district-rwanda
#3
Eugene Mazimpaka, Francis Mbuza, Tukei Michael, Eugene N Gatari, E M Bukenya, Okwee-Acai James
A study was conducted to characterize the cattle production systems in Nyagatare District, Eastern Province of Rwanda using pre-tested questionnaires, interviews with key informants as well as focus group discussions in a period of 2 months. The respondents were selected by multi-stage sampling at sector and cell levels. Based on the procedure of Krejcie and Morgan (Educational and Psychological Measurement 30:607-610, 1970) to determine the overall sample size, the result indicated that the majority (98.3%) of farms were privately owned by large families of five to seven members, and most farmers (53...
August 19, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28822945/development-of-an-accurate-and-sensitive-method-for-lactate-analysis-in-exhaled-breath-condensate-by-lc-ms-ms
#4
Thomas C Jackson, Yan Victoria Zhang, Patricia J Sime, Richard P Phipps, R Matthew Kottmann
Exhaled breath condensate (EBC) is easily obtained for clinical diagnosis and prognosis for pulmonary diseases and has gained much interest in biomarker discovery research and studies. Lactate, a physiological material, is found in EBC and has been demonstrated to be a potential indicator of chronic obstructive pulmonary disease and other lung diseases. Several assays are available to detect lactate in human body fluids, and yet none is suitable for detecting lactate in EBC. Due to the very low concentration of lactate in EBC and low volume of EBC, it is very important to develop an assay to measure lactate with high sensitivity, accuracy and easy sample processing...
July 24, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28822855/tetrandrine-cardioprotection-in-ischemia-reperfusion-i-r-injury-via-jak3-stat3-hexokinase-ii
#5
Tie-Jun Zhang, Rui-Xia Guo, Xia Li, Yan-Wei Wang, Yong-Jun Li
Tetrandrine(TET), a bisbenzylisoquinoline alkaloid has been used for the treatment of cardiovascular diseases and hypertension. This study was to investigate whether tetrandrine exerts cardioprotection in ischemia-reperfusion (I/R) injury and the mechanisms involved. The cardioprotection effect and mechanisms of tetrandrine was evaluated by I/R injury cardiac cell model. Hexokinase II (HKII) is the critical regulators of mitochondrial dysfunction in cardiac I/R injury and it participate in the regulation of glycolysis and energy metabolism...
August 16, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28822853/intrauterine-and-lactation-exposure-to-fluoxetine-blunted-in-the-offspring-the-aortic-adaptive-response-induced-by-acute-restraint-stress
#6
Bruno V D Marques, Carolina M Higashi, Daniella R B da S Novi, Nagela G Zanluqui, Thais F Gregório, Phileno Pinge-Filho, Daniela C C Gerardin, Gislaine P Gomes, Estefânia G Moreira, Graziela S Ceravolo
Selective serotonin reuptake inhibitors are the most widely prescribed antidepressants to women during pregnancy. Maternal treatment with fluoxetine can expose fetuses and neonates to higher levels of serotonin that plays a role in stress response. Thus, the aim of the study was to evaluate whether maternal treatment with fluoxetine interferes with aorta reactivity of adult male offspring after acute restraint stress. Wistar rats were gavaged with fluoxetine (5mg/kg/day) or water (control) during pregnancy and lactation...
August 16, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28822783/the-expression-induction-and-pharmacological-activity-of-cyp1a2-are-post-transcriptionally-regulated-by-microrna-hsa-mir-132-5p
#7
Yinting Chen, Linjuan Zeng, Yong Wang, William H Tolleson, Bridgett Knox, Si Chen, Zhen Ren, Lei Guo, Nan Mei, Feng Qian, Kaihong Huang, David Liu, Weida Tong, Dianke Yu, Baitang Ning
Cytochrome P450 1A2 (CYP1A2) is one of the most abundant and important drug metabolizing enzymes in human liver. However, little is known about the post-transcriptional regulation of CYP1A2, especially the mechanisms involving microRNAs (miRNAs). This study applied a systematic approach to investigate the post-transcriptional regulation of CYP1A2 by miRNAs. Candidate miRNAs targeting the 3'-untranslated region (3'-UTR) of CYP1A2 were screened in silico, resulting in the selection of sixty-two potential miRNAs for further analysis...
August 16, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28822552/consequences-of-dietary-energy-source-and-energy-level-on-energy-balance-lactogenic-hormones-and-lactation-curve-characteristics-of-cows-after-a-short-or-omitted-dry-period
#8
R J van Hoeij, J Dijkstra, R M Bruckmaier, J J Gross, T J G M Lam, G J Remmelink, B Kemp, A T M van Knegsel
Omitting the dry period (DP) generally reduces milk production in the subsequent lactation. The aim of this study was to evaluate the effect of dietary energy source-glucogenic (G) or lipogenic (L)-and energy level-standard (std) or low-on milk production; energy balance (EB); lactogenic hormones insulin, insulin-like growth factor 1 (IGF-1), and growth hormone (GH); and lactation curve characteristics between wk 1 and 44 postpartum in cows after a 0-d or 30-d DP. Cows (n = 110) were assigned randomly to 3 transition treatments: a 30-d DP with a standard energy level required for expected milk yield [30-d DP(std)], a 0-d DP with the same energy level as cows with a 30-d DP [0-d DP(std)], and a 0-d DP with a low energy level [0-d DP(low)]...
August 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28822545/effects-of-the-dose-and-viability-of-saccharomyces-cerevisiae-2-ruminal-fermentation-performance-of-lactating-dairy-cows-and-correlations-between-ruminal-bacteria-abundance-and-performance-measures
#9
Y Jiang, I M Ogunade, K G Arriola, M Qi, D Vyas, C R Staples, A T Adesogan
This study examined effects of the dose and viability of supplemented Saccharomyces cerevisiae yeast strain YE1496 on ruminal fermentation and performance of lactating dairy cows. A second objective was to examine correlations between ruminal bacteria abundance and performance measures. Four ruminally cannulated lactating cows (284 ± 18 days in milk) were assigned randomly to 1 of 4 treatment sequences in a 4 × 4 Latin square experimental design using four 21-d experimental periods. Cows were fed a nonacidotic total mixed ration comprising 22...
August 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28822229/vasculogenesis-and-angiogenesis-initiation-under-normoxic-conditions-through-wnt-%C3%AE-catenin-pathway-in-gliomas
#10
Alexandre Vallée, Rémy Guillevin, Jean-Noël Vallée
The canonical Wnt/β-catenin pathway is up-regulated in gliomas and involved in proliferation, invasion, apoptosis, vasculogenesis and angiogenesis. Nuclear β-catenin accumulation correlates with malignancy. Hypoxia activates hypoxia-inducible factor (HIF)-1α by inhibiting HIF-1α prolyl hydroxylation, which promotes glycolytic energy metabolism, vasculogenesis and angiogenesis, whereas HIF-1α is degraded by the HIF prolyl hydroxylase under normoxic conditions. We focus this review on the links between the activated Wnt/β-catenin pathway and the mechanisms underlying vasculogenesis and angiogenesis through HIF-1α under normoxic conditions in gliomas...
August 19, 2017: Reviews in the Neurosciences
https://www.readbyqxmd.com/read/28821380/unique-transcriptional-profile-of-native-persisters-in-escherichia-coli
#11
Shinya Matsumoto, Yuto Kawai, Satoshi Miyagawa, Yuka Iwamoto, Shujiro Okuda, Alicia Sánchez-Gorostiaga, Miguel Vicente, Satoshi Tsuneda
Non-dividing persisters, bacteria that can survive in the presence of antibiotics by pausing their metabolic activity, are among the many causes of the refractory nature of bacterial infections. Here we constructed a recombinant Escherichia coli strain that enables to distinguish non-dividing from dividing cell based on Z-ring during cell division. Then, non-dividing cells and dividing cells were successfully separated using a fluorescence activated cell sorter. The sorted non-dividing cells showed significantly higher tolerance toward ofloxacin than dividing cells, which indicates that persisters were concentrated with the methodology...
August 15, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28821352/towards-optimisation-of-induced-pluripotent-cell-culture-extracellular-acidification-results-in-growth-arrest-of-ipsc-prior-to-nutrient-exhaustion
#12
Anja Wilmes, Caroline Rauch, Giada Carta, Georg Kern, Florian Meier, Wilfried Posch, Doris Wilflingseder, Lyle Armstrong, Majlinda Lako, Mario Beilmann, Gerhard Gstraunthaler, Paul Jennings
Human induced pluripotent stem cells (iPSC) have the potential to radically reduce the number of animals used in both toxicological science and disease elucidation. One initial obstacle culturing iPSC is that they require daily medium exchange. This study attempts to clarify why and propose some practical solutions. Two iPSC lineages were fed at different intervals in a full growth area (FGA) or a restricted growth area (RGA). The FGA consisted of a well coated with Matrigel™ and the RGA consisted of a coated coverslip placed in a well...
August 15, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28821318/consumption-of-an-acid-protease-derived-from-aspergillus-oryzae-causes-bifidogenic-effect-in-rats
#13
Yongshou Yang, Akane Iwamoto, Thanutchaporn Kumrungsee, Yukako Okazaki, Manabu Kuroda, Shotaro Yamaguchi, Norihisa Kato
A marked elevation in the abundance of Bifidobacterium was found in the cecum of rats that were fed a high-fat diet supplemented with an Amano protease preparation (derived from Aspergillus oryzae). The protease preparation contains several digestive enzymes, including acid protease (AcP), alkaline protease, and amylase. We hypothesized that the elevation in the abundance of Bifidobacterium by Amano protease preparation is associated with the digestive enzymes involved in the protease preparation. To test this hypothesis, this study was conducted to investigate if such bifidogenic effect is because of the AcP...
August 2017: Nutrition Research
https://www.readbyqxmd.com/read/28820965/systematic-identification-of-mcu-modulators-by-orthogonal-interspecies-chemical-screening
#14
Daniela M Arduino, Jennifer Wettmarshausen, Horia Vais, Paloma Navas-Navarro, Yiming Cheng, Anja Leimpek, Zhongming Ma, Alba Delrio-Lorenzo, Andrea Giordano, Cecilia Garcia-Perez, Guillaume Médard, Bernhard Kuster, Javier García-Sancho, Dejana Mokranjac, J Kevin Foskett, M Teresa Alonso, Fabiana Perocchi
The mitochondrial calcium uniporter complex is essential for calcium (Ca(2+)) uptake into mitochondria of all mammalian tissues, where it regulates bioenergetics, cell death, and Ca(2+) signal transduction. Despite its involvement in several human diseases, we currently lack pharmacological agents for targeting uniporter activity. Here we introduce a high-throughput assay that selects for human MCU-specific small-molecule modulators in primary drug screens. Using isolated yeast mitochondria, reconstituted with human MCU, its essential regulator EMRE, and aequorin, and exploiting a D-lactate- and mannitol/sucrose-based bioenergetic shunt that greatly minimizes false-positive hits, we identify mitoxantrone out of more than 600 clinically approved drugs as a direct selective inhibitor of human MCU...
August 17, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28820957/the-association-between-pregnancy-intention-and-breastfeeding
#15
Shimrit Keddem, Rosemary Frasso, Melissa Dichter, Alexandra Hanlon
BACKGROUND: Although breastfeeding is associated with proven benefits to both mother and child, there are many factors that influence a mother's decision to breastfeed. Pregnancy intentionality at the time of conception is associated with postpartum maternal behavior including breastfeeding. Research aim: We sought to understand how maternal and paternal pregnancy intentions were associated with breastfeeding initiation and duration in a nationally representative sample. METHODS: We used a cross-sectional, retrospective study of the CDC National Survey of Family Growth data to examine the link between pregnancy intentionality and breastfeeding initiation and duration among women ages 15 to 44 years...
August 1, 2017: Journal of Human Lactation: Official Journal of International Lactation Consultant Association
https://www.readbyqxmd.com/read/28820956/breastfeeding-support-for-criminalized-women-in-canada
#16
Martha Jane Paynter, Erna Snelgrove-Clarke
No abstract text is available yet for this article.
August 1, 2017: Journal of Human Lactation: Official Journal of International Lactation Consultant Association
https://www.readbyqxmd.com/read/28820954/lactation-newsmakers-an-interview-with-felicity-m-a-savage-ma-bm-bch-frcp-frcpch-ffph-fiap-fabm
#17
Michele K Griswold, Amal Omer Salim
No abstract text is available yet for this article.
August 1, 2017: Journal of Human Lactation: Official Journal of International Lactation Consultant Association
https://www.readbyqxmd.com/read/28820951/lactation-consultants-perceived-barriers-to-providing-professional-breastfeeding-support
#18
Erica H Anstey, Martha Coulter, Cecilia M Jevitt, Kay M Perrin, Sharon Dabrow, Lynne B Klasko-Foster, Ellen M Daley
BACKGROUND: Addressing suboptimal breastfeeding initiation and duration rates is a priority in the United States. To address challenges to improving these rates, the voices of the providers who work with breastfeeding mothers should be heard. Research aim: The purpose of this study was to explore lactation consultants' perceived barriers to managing early breastfeeding problems. METHODS: This qualitative study was conducted with a grounded theory methodological approach...
August 1, 2017: Journal of Human Lactation: Official Journal of International Lactation Consultant Association
https://www.readbyqxmd.com/read/28820737/when-one-disease-is-not-enough-succinyl-coa-3-oxoacid-coenzyme-a-transferase-scot-deficiency-due-to-a-novel-mutation-in-oxct1-in-an-infant-with-known-phenylketonuria
#19
Jan-Niclas Schwade, Matthias Endmann, Thomas Hofmann, Stephan Rust, Jörn Oliver Sass, Frank Rutsch
A 9-month-old Turkish girl was admitted several times within 3 months to the hospital in reduced general condition and with extreme tachypnea. The patient had been diagnosed with phenylketonuria (PKU) in newborn screening and has been treated with a low phenylalanine diet and amino acid supplements. Each time an unexplained pronounced metabolic acidosis was noted, and the child was treated with sodium-bicarbonate and glucose-electrolyte infusions. The acidosis with only slightly abnormal glucose, normal lactate levels and pronounced ketonuria suggested a defect in ketone body utilization...
August 18, 2017: Journal of Pediatric Endocrinology & Metabolism: JPEM
https://www.readbyqxmd.com/read/28820632/media-presentation-of-breastfeeding-beliefs-in-newspapers
#20
Rose Hitt, Jie Zhuang, Jennifer Anderson
Despite numerous health benefits for babies and mothers, many women do not either initiate or continue breastfeeding for the recommended duration, and increasing breastfeeding is a national priority. It is important to understand media messages on the topic, given that breastfeeding is influenced by many environmental factors and that perceived norms, social support, and perceptions of difficulties predict breastfeeding. The current study analyzes how media covers (1) breastfeeding in general, (2) public breastfeeding, and (3) extended breastfeeding (past 1 year)...
August 18, 2017: Health Communication
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