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https://www.readbyqxmd.com/read/28647649/recovery-after-pilp-remineralization-of-dentin-lesions-created-with-two-cariogenic-acids
#1
K Saeki, Y-C Chien, G Nonomura, A F Chin, S Habelitz, L B Gower, S J Marshall, G W Marshall
OBJECTIVES: Acetate and lactate are important cariogenic acids produced by oral bacteria. They produced different residual dentin structures in artificial lesions of similar depth. We evaluated if such lesions responded in the same way to a polymer-induced-liquid-precursor (PILP) remineralization. DESIGN: Dentin blocks obtained from human third molars, divided into 6 groups (n=3). Blocks were demineralized with acetate (66h) or lactate (168h) buffer at pH 5.0 to create 140μm target lesion depths...
June 15, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28647528/the-linkage-between-nutrient-supply-intracellular-enzyme-abundances-and-bacterial-growth-new-evidences-from-the-central-carbon-metabolism-of-corynebacterium-glutamicum
#2
Stephan Noack, Raphael Voges, Jochem Gätgens, Wolfgang Wiechert
Corynebacterium glutamicum serves as important production host for small molecular compounds that are derived from precursor molecules of the central carbon metabolism. It is therefore a well-studied model organism of industrial biotechnology. However, a deeper understanding of the regulatory principles underlying the synthesis of central metabolic enzymes under different environmental conditions as well as its impact on cell growth is still missing. We studied enzyme abundances in C. glutamicum in response to growth on: i) one limiting carbon source by sampling chemostat and fed-batch cultivations and ii) changing carbon sources provided in excess by sampling batch cultivations...
June 21, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28647476/substance-p-promotes-hepatic-stellate-cell-proliferation-and-activation-via-the-tgf-%C3%AE-1-smad-3-signaling-pathway
#3
Lei Peng, Xiaoqing Jia, Jianjian Zhao, Ruibing Cui, Ming Yan
Prolonged activation and proliferation of hepatic stellate cells (HSCs) usually results in the initiation and progression of liver fibrosis following injury. Recent studies have shown that Substance P (SP) participates in the development of fibrosis. However, whether SP is involved in liver fibrosis, especially in the activation and proliferation of HSCs, is largely unknown. In the present study, we measured the effects of a series of concentrations of SP on the cell viability and activation of HSC-T6 cells and LX2 cells...
June 21, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28647407/external-validation-of-the-simple-null-please-clinical-score-in-predicting-outcome-of-out-of-hospital-cardiac-arrest
#4
Tatjana S Potpara, Miroslav Mihajlovic, Sanja Stankovic, Tanja Jozic, Irena Jozic, Milika R Asanin, Rajai Ahmad, Gregory Y H Lip
BACKGROUND: Rapid clinical decision-making on further management of patients with out-of-hospital cardiac arrest may be challenging. Recently, a 'futility' score (NULL-PLEASE) incorporating multiple adverse resuscitation features (Non-shockable rhythm, Unwitnessed arrest, Long no-flow or Long low-flow period, blood pH <7.2, Lactate >7.0 mmol/l, End-stage chronic kidney disease on dialysis, Age ≥85 years, Still resuscitation and Extra-cardiac cause) has been proposed to help identify out-of-hospital cardiac arrest patients unlikely to survive; however, external independent score validation is lacking...
June 21, 2017: American Journal of Medicine
https://www.readbyqxmd.com/read/28647336/milk-composition-and-health-status-from-mammary-gland-quarters-adjacent-to-glands-affected-with-naturally-occurring-clinical-mastitis
#5
Marcel G Paixão, Luiz R Abreu, Roxann Richert, Pamela L Ruegg
Mammary gland quarters have usually been considered to be anatomically and physiologically independent, but some recent research has indicated more interdependence than previously reported. The objective of this study was to compare milk composition (fat, total protein, lactose, solids-not-fat, and chloride) and health status (somatic cell count, differential leukocyte count, and lactate dehydrogenase) of milk samples from unaffected mammary glands of an udder with a single clinically inflamed quarter to results of milk samples from healthy mammary glands of healthy cows...
June 21, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28647335/incidence-and-duration-of-increased-somatic-cell-count-in-swedish-dairy-cows-and-associations-with-milking-system-type
#6
J Frössling, A Ohlson, C Hallén-Sandgren
Mastitis is one of the most costly diseases in dairy cows worldwide. Increased somatic cell count (SCC) is an indication of mastitis, often subclinical, which implies bacterial infection without clinical signs of inflammation. The aim was to investigate the occurrence of elevated udder SCC (defined as ≥200,000 cells/mL) over the lactation period, and before and after the dry period, for cows of different parity. The aim was also to analyze the association between prevalence and incidence of increased udder SCC and information on cow and herd level, such as breed and milking system type...
June 21, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28647333/effects-of-bedding-with-recycled-sand-on-lying-behaviors-udder-hygiene-and-preference-of-lactating-holstein-dairy-cows
#7
J A Kull, H D Ingle, R A Black, N L Eberhart, P D Krawczel
Effects of bedding with recycled sand and season on lying behaviors, hygiene, and preferences of late-lactation Holstein cows were studied. It was hypothesized that recycled sand will decrease lying time and increase hygiene scores due to increased moisture content and organic matter, and thus a preference for the control sand will be evident. Cows (n = 64) were divided into 4 groups (n = 8 per group) per season. In summer (August to September), cows were balanced by days in milk (268.1 ± 11.9 d) and parity (2...
June 21, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28647080/are-capillary-and-arterial-lactates-interchangeable
#8
EDITORIAL
François Dépret, Matthieu Legrand
No abstract text is available yet for this article.
June 2017: Anaesthesia, Critical Care & Pain Medicine
https://www.readbyqxmd.com/read/28646721/biochemical-immunological-hybrid-biosensor-based-on-two-dimensional-chromatography-for-on-site-sepsis-diagnosis
#9
Seung-Wan Kim, Il-Hoon Cho, Guei-Sam Lim, Gi-Na Park, Se-Hwan Paek
A hybrid-biosensor system that can simultaneously fulfill the immunoassay for protein markers (e.g., C-reactive protein (CRP) and procalcitonin (PCT)) and the enzyme assay for metabolic substances (e.g., lactate) in the same sepsis-based sample has been devised. Such a challenge was pursued through the installation of an enzyme-reaction zone on the signal pad of the typical immuno-strip for the rapid two-dimensional (2-D)-chromatography test. To minimize the mutual interference in the hybrid assays, a pre-determined membrane site was etched in a pattern and mounted with a biochemical-reaction pad, thereby allowing a loaded sample to enter and then stay in the pad for a colored-signal production over the course of an immunoassay...
June 16, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28646647/successful-maintenance-of-key-physiological-parameters-in-preterm-lambs-treated-with-ex%C3%A2-vivo-uterine-environment-therapy-for-a-period-of-1-week
#10
Haruo Usuda, Shimpei Watanabe, Yuichiro Miura, Masatoshi Saito, Gabrielle C Musk, Judith Rittenschober-Böhm, Hideyuki Ikeda, Shinichi Sato, Takushi Hanita, Tadashi Matsuda, Alan H Jobe, John P Newnham, Sarah J Stock, Matthew W Kemp
BACKGROUND: Extremely preterm infants born at the border of viability (22-24 weeks' gestation) have high rates of death and lasting disability. Ex vivo uterine environment therapy is an experimental neonatal intensive care strategy that provides gas exchange using parallel membranous oxygenators connected to the umbilical vessels, sparing the extremely preterm cardiopulmonary system from ventilation-derived injury. OBJECTIVE: In this study, we aimed to refine our ex vivo uterine environment therapy platform to eliminate fetal infection and inflammation, while simultaneously extending the duration of hemodynamically stable ex vivo uterine environment therapy to 1 week...
June 20, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28646537/explaining-infant-feeding-the-role-of-previous-personal-and-vicarious-experience-on-attitudes-subjective-norms-self-efficacy-and-breastfeeding-outcomes
#11
Naomi C Bartle, Kate Harvey
OBJECTIVES: Breastfeeding confers important health benefits to both infants and their mothers, but rates are low in the United Kingdom and other developed countries despite widespread promotion. This study examined the relationships between personal and vicarious experience of infant feeding, self-efficacy, the theory of planned behaviour variables of attitudes and subjective norm, and the likelihood of breastfeeding at 6-8 weeks post-natally. DESIGN: A prospective questionnaire study of both first-time mothers (n = 77) and experienced breastfeeders (n = 72) recruited at an antenatal clinic in South East England...
June 23, 2017: British Journal of Health Psychology
https://www.readbyqxmd.com/read/28646020/mcam-mediates-chemoresistance-in-small-cell-lung-cancer-via-the-pi3k-akt-sox2-signaling-pathway
#12
Satyendra C Tripathi, Johannes F Fahrmann, Muge Celiktas, Mitzi Aguilar, Kieren D Marini, Mohit Kumar Jolly, Hiroyuki Katayama, Hong Wang, Eunice N Murage, Jennifer B Dennison, D Neil Watkins, Herbert Levine, Edwin J Ostrin, Ayumu Taguchi, Samir M Hanash
Despite favorable responses to initial therapy, small cell lung cancer (SCLC) relapse occurs within a year and exhibits resistance to multiple drugs. Due to limited accessibility of patient tissues for research purposes, SCLC-patient derived xenografts (PDX) have provided the best opportunity to address this limitation. Here we sought to identify novel mechanisms involved in SCLC chemoresistance. Through in-depth proteomic profiling, we identified MCAM as a markedly upregulated surface receptor in chemoresistant SCLC cell lines and in chemoresistant PDX compared to matched treatment-naïve tumors...
June 23, 2017: Cancer Research
https://www.readbyqxmd.com/read/28645949/double-blind-randomised-controlled-trial-of-the-independent-and-synergistic-effect-of-spirulina-maxima-with-exercise-isese-on-general-fitness-lipid-profile-and-redox-status-in-overweight-and-obese-subjects-study-protocol
#13
Marco Antonio Hernández-Lepe, José Alberto López-Díaz, Laura Alejandra de la Rosa, Rosa Patricia Hernández-Torres, Abraham Wall-Medrano, Marco Antonio Juarez-Oropeza, José Pedraza-Chaverri, Rene Urquidez-Romero, Arnulfo Ramos-Jiménez
INTRODUCTION: In order to reduce cardiovascular disease risk factors, a healthy diet must include dietary antioxidants from different sources (eg, Spirulina maxima) and regular practice of exercise should be promoted. There is some evidence from animal studies that S. maxima and exercise decrease cardiovascular disease risks factors. However, very few studies have proved the independent or synergistic effect of S. maxima plus exercise in humans. This study attempts to address the independent and synergistic effects in overweight and obese subjects participating in a systematic physical exercise programme at moderate intensity on general fitness, plasma lipid profile and antioxidant capacity...
June 23, 2017: BMJ Open
https://www.readbyqxmd.com/read/28645882/cardiopreventive-effect-of-ethanolic-extract-of-date-palm-pollen-against-isoproterenol-induced-myocardial-infarction-in-rats-through-the-inhibition-of-the-angiotensin-converting-enzyme
#14
Amal Daoud, Fedia Ben Mefteh, Kais Mnafgui, Mouna Turki, Salwa Jmal, Rawdha Ben Amar, Fatma Ayadi, Abdelfattah ElFeki, Leila Abid, Mostafa E Rateb, Lassaad Belbahri, Adel Kadri, Neji Gharsallah
The present study aimed to examine the putative preventive effect of the ethanolic extract Date Palm Pollen (DPP, Phoenix dactylifera L., family Arecaceae) on isoproterenol-induced myocardial infarction (MI) in rats. Twenty four rats were randomly divided into four groups including control. They were treated with DPP extract (400mg/kg) and clopidogrel (0.2mg/kg) for 7days followed by myocardial injury induction using subcutaneous isoproterenol (100mg/kg) with an interval of 24h for two days (6th and 7th day)...
June 20, 2017: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
https://www.readbyqxmd.com/read/28645691/maternal-exposure-to-a-mixture-of-persistent-organic-pollutants-pops-affects-testis-histology-epididymal-sperm-count-and-induces-sperm-dna-fragmentation-in-mice
#15
Abdolrahman Khezri, Birgitte Lindeman, Anette K Krogenæs, Hanne F Berntsen, Karin E Zimmer, Erik Ropstad
Persistent organic pollutants (POPs) are widespread throughout the environment and some are suspected to induce reproductive toxicity. As animals and humans are exposed to complex mixtures of POPs, it is reasonable to assess how such mixtures could interact with the reproductive system. Our aim is to investigate how maternal exposure to a mixture of 29 different persistent organic pollutants, formulated to mimic the relative POP levels in the food basket of the Scandinavian population, could alter reproductive endpoints...
June 20, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28645576/mechanisms-of-hepatocellular-toxicity-associated-with-new-psychoactive-synthetic-cathinones
#16
Dino Luethi, Matthias E Liechti, Stephan Krähenbühl
Synthetic cathinones are a new class of psychostimulant substances. Rarely, they can cause liver injury but associated mechanisms are not completely elucidated. In order to increase our knowledge about mechanisms of hepatotoxicity, we investigated the effect of five frequently used cathinones on two human cell lines. Bupropion was included as structurally related drug used therapeutically. In HepG2 cells, bupropion, MDPV, mephedrone and naphyrone depleted the cellular ATP content at lower concentrations (0...
June 20, 2017: Toxicology
https://www.readbyqxmd.com/read/28645525/placenta-as-alternative-specimen-to-detect-in-utero-cannabis-exposure-a-systematic-review-of-the-literature
#17
REVIEW
Daniela Marchetti, Gerardo Di Masi, Francesca Cittadini, Giuseppe La Monaca, Nadia De Giovanni
The use of cannabis during pregnancy and lactation is widely recognized as a health concern. The placenta is one of the several biological matrices that can be used to detect in utero drug exposure with the distinct advantage of easy and noninvasive collection. We conducted a PubMed search of selected literature published to June 30, 2016 to investigate the pharmacokinetics of cannabinoids, the active constituent of cannabis, in the placenta. The majority of the selected twelve papers was before 1990 and concerned animals at term gestation with great variability of experimental conditions...
June 20, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28645299/programming-of-mouse-obesity-by-maternal-exposure-to-concentrated-ambient-fine-particles
#18
Minjie Chen, Xiaoke Wang, Ziying Hu, Huifen Zhou, Yanyi Xu, Lianglin Qiu, Xiaobo Qin, Yuhao Zhang, Zhekang Ying
BACKGROUND: Many diseases including obesity may originate through alterations in the early-life environment that interrupts fetal development. Increasing evidence has shown that exposure to ambient fine particles (PM2.5) is associated with abnormal fetal development. However, its long-term metabolic effects on offspring have not been systematically investigated. RESULTS: To determine if maternal exposure to PM2.5 programs offspring obesity, female C57Bl/6j mice were exposed to filtered air (FA) or concentrated ambient PM2...
June 23, 2017: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/28645294/3-5-4-trihydroxy-6-7-3-trimethoxyflavone-protects-against-beta-amyloid-induced-neurotoxicity-through-antioxidative-activity-and-interference-with-cell-signaling
#19
Alona Telerman, Rivka Ofir, Yoel Kashman, Anat Elmann
BACKGROUND: Alzheimer's disease is a neurodegenerative disease, characterized by progressive decline in memory and cognitive functions, that results from loss of neurons in the brain. Amyloid beta (Aβ) protein and oxidative stress are major contributors to Alzheimer's disease, therefore, protecting neuronal cells against Aβ-induced toxicity and oxidative stress might form an effective approach for treatment of this disease. 3,5,4'-trihydroxy-6,7,3'-trimethoxyflavone (TTF) is a flavonoid we have purified from the plant Achillea fragrantissima; and the present study examined, for the first time, the effects of this compound on Aβ-toxicity to neuronal cells...
June 23, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28645283/oocyte-polarized-light-microscopy-assay-of-specific-follicular-fluid-metabolites-and-gene-expression-in-cumulus-cells-as-different-approaches-to-predict-fertilization-efficiency-after-icsi
#20
Alberto Revelli, Stefano Canosa, Loredana Bergandi, Oleksii A Skorokhod, Valentina Biasoni, Andrea Carosso, Angela Bertagna, Milena Maule, Elisabetta Aldieri, Maria Diletta D'Eufemia, Francesca Evangelista, Nicola Colacurci, Chiara Benedetto
BACKGROUND: The complex relationship between oocyte morphology, specific follicular fluid metabolites, gene expression in cumulus granulosa cells, and oocyte competence toward fertilization and embryo development still needs further clarification. METHODS: Forty-six oocytes retrieved from the largest pre-ovulatory follicle of patients undergoing intra-cytoplasmic sperm injection (ICSI) were considered assessing: (a) oocyte morphological characteristics at polarized light microscopy (PLM), (b) specific follicular fluid (FF) metabolites previously suggested to influence oocyte competence (AMH, markers of redox status and of cytotoxicity), (c) transcription of AMH and AMH type II receptor genes in cumulus cells...
June 23, 2017: Reproductive Biology and Endocrinology: RB&E
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