keyword
MENU ▼
Read by QxMD icon Read
search

Bacteriophage diagnostic

keyword
https://www.readbyqxmd.com/read/29299830/review-of-the-nature-diversity-and-structure-of-bacteriophage-receptor-binding-proteins-that-target-gram-positive-bacteria
#1
REVIEW
Ahmed S A Dowah, Martha R J Clokie
As the importance of bacteriophages as novel antimicrobials and potential diagnostics comes increasingly into focus, there is a heightened interest in understanding the mechanisms of how they interact with their bacterial hosts. The first step of a bacteriophage (phage) infection is the recognition of specific moieties on the bacterial cell surface as determined by their phage receptor binding proteins (RBPs). Knowledge of RBPs and how they interact with bacteria has been driven by studies of model phages and of industrially important phages, such as those that impact the dairy industry...
January 3, 2018: Biophysical Reviews
https://www.readbyqxmd.com/read/29298913/cross-genus-rebooting-of-custom-made-synthetic-bacteriophage-genomes-in-l-form-bacteria
#2
Samuel Kilcher, Patrick Studer, Christina Muessner, Jochen Klumpp, Martin J Loessner
Engineered bacteriophages provide powerful tools for biotechnology, diagnostics, pathogen control, and therapy. However, current techniques for phage editing are experimentally challenging and limited to few phages and host organisms. Viruses that target Gram-positive bacteria are particularly difficult to modify. Here, we present a platform technology that enables rapid, accurate, and selection-free construction of synthetic, tailor-made phages that infect Gram-positive bacteria. To this end, custom-designed, synthetic phage genomes were assembled in vitro from smaller DNA fragments...
January 3, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29298884/genomic-and-biochemical-characterization-of-acinetobacter-podophage-petty-reveals-a-novel-lysis-mechanism-and-tail-associated-depolymerase-activity
#3
A C Hernandez-Morales, L L Lessor, T L Wood, D Migl, E M Mijalis, W K Russell, R F Young, J J Gill
The increased prevalence of drug-resistant, nosocomial Acinetobacter infections, particularly from pathogenic members of the Acinetobacter calcoaceticus-baumannii complex, necessitates the exploration of novel treatments such as phage therapy. In the present study, we characterize phage Petty, a novel podophage that infects multidrug-resistant Acinetobacter nosocomialis and Acinetobacter baumannii Genome analysis reveals that phage Petty is a 40,431bp ϕKMV-like phage, with a coding density of 92.2% and a G+C content of 42...
January 3, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29259289/resolution-of-habitat-associated-ecogenomic-signatures-in-bacteriophage-genomes-and-application-to-microbial-source-tracking
#4
Lesley A Ogilvie, Jonathan Nzakizwanayo, Fergus M Guppy, Cinzia Dedi, David Diston, Huw Taylor, James Ebdon, Brian V Jones
Just as the expansion in genome sequencing has revealed and permitted the exploitation of phylogenetic signals embedded in bacterial genomes, the application of metagenomics has begun to provide similar insights at the ecosystem level for microbial communities. However, little is known regarding this aspect of bacteriophage associated with microbial ecosystems, and if phage encode discernible habitat-associated signals diagnostic of underlying microbiomes. Here we demonstrate that individual phage can encode clear habitat-related 'ecogenomic signatures', based on relative representation of phage-encoded gene homologues in metagenomic data sets...
December 19, 2017: ISME Journal
https://www.readbyqxmd.com/read/29232619/engineering-of-protein-nanopores-for-sequencing-chemical-or-protein-sensing-and-disease-diagnosis
#5
REVIEW
Shaoying Wang, Zhengyi Zhao, Farzin Haque, Peixuan Guo
Biological systems contain highly-ordered structures performing diverse functions. The elegant structures of biomachines have inspired the development of nanopores as single molecule sensors. Over the years, the utility of nanopores for detecting a wide variety of analytes have rapidly emerged for sensing, sequencing and diagnostic applications. Several protein channels with diverse shapes and sizes, such as motor channels from bacteriophage Phi29, SPP1, T3, and T4, as well as α-hemolysin, MspA, aerolysin, FluA, OmpF/G, CsgG, ClyA, have been continually investigated and developed as nanopores...
December 9, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/29163915/field-deployable-quantitative-rapid-identification-of-active-ebola-virus-infection-in-unprocessed-blood
#6
Kavit Shah, Emma Bentley, Adam Tyler, Kevin S R Richards, Edward Wright, Linda Easterbrook, Diane Lee, Claire Cleaver, Louise Usher, Jane E Burton, James K Pitman, Christine B Bruce, David Edge, Martin Lee, Nelson Nazareth, David A Norwood, Sterghios A Moschos
The West African Ebola virus outbreak underlined the importance of delivering mass diagnostic capability outside the clinical or primary care setting in effectively containing public health emergencies caused by infectious disease. Yet, to date, there is no solution for reliably deploying at the point of need the gold standard diagnostic method, real time quantitative reverse transcription polymerase chain reaction (RT-qPCR), in a laboratory infrastructure-free manner. In this proof of principle work, we demonstrate direct performance of RT-qPCR on fresh blood using far-red fluorophores to resolve fluorogenic signal inhibition and controlled, rapid freeze/thawing to achieve viral genome extraction in a single reaction chamber assay...
November 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28985502/the-revolution-continues-newly-discovered-systems-expand-the-crispr-cas-toolkit
#7
REVIEW
Karthik Murugan, Kesavan Babu, Ramya Sundaresan, Rakhi Rajan, Dipali G Sashital
CRISPR-Cas systems defend prokaryotes against bacteriophages and mobile genetic elements and serve as the basis for revolutionary tools for genetic engineering. Class 2 CRISPR-Cas systems use single Cas endonucleases paired with guide RNAs to cleave complementary nucleic acid targets, enabling programmable sequence-specific targeting with minimal machinery. Recent discoveries of previously unidentified CRISPR-Cas systems have uncovered a deep reservoir of potential biotechnological tools beyond the well-characterized Type II Cas9 systems...
October 5, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28819114/isothermal-amplification-of-long-discrete-dna-fragments-facilitated-by-single-stranded-binding-protein
#8
Yinhua Zhang, Nathan A Tanner
Isothermal amplification methods for detection of DNA and RNA targets have expanded significantly in recent years, promising a new wave of simple and rapid molecular diagnostics. Current isothermal methods result in the generation of short fragments (<150 base pairs) or highly branched long DNA products. Here we report the amplification of discrete target fragments of several kilobases at 37 °C from both double- and single-stranded circular template DNA using specific primer pairs. In contrast to existing methods, this amplification requires only the single-stranded DNA-binding protein gp32 from bacteriophage T4 and a strand-displacing DNA polymerase...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28757387/a-fast-pcr-based-method-for-the-characterization-of-prophage-profiles-in-strains-of-the-lactobacillus-casei-group
#9
Delfina Zaburlin, Diego J Mercanti, Andrea Quiberoni
Lysogeny is widespread among Lactobacillus strains of the casei group (L. casei, L. paracasei and L. rhamnosus), and prophages account for most strain-specific DNA. Numerous PCR based methods have been developed to detect free phages of lactic acid bacteria, but they do not take in consideration prophages. In this study, a new PCR method for the detection of lysogeny was developed using genome sequences of L. casei group strains (including BL23) and bacteriophages. Nine pairs of primers were designed to selectively amplify the highly conserved prophage iA2 (pairs #1-#3) and fragments of two groups phages of temperate origin: CL1/CL2/iLp1308/iLp84 (pairs #4 and #5) and Lrm1/J-1/PL-1/A2/AT3/Lc-Nu (pairs #6 to #9)...
October 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28750539/octupolar-metastructures-for-a-highly-sensitive-rapid-and-reproducible-phage-based-detection-of-bacterial-pathogens-by-surface-enhanced-raman-scattering
#10
Massimo Rippa, Riccardo Castagna, Marianna Pannico, Pellegrino Musto, Giorgia Borriello, Rubina Paradiso, Giorgio Galiero, Sergio Bolletti Censi, Jun Zhou, Joseph Zyss, Lucia Petti
The development of fast and ultrasensitive methods to detect bacterial pathogens at low concentrations is of high relevance for human and animal health care and diagnostics. In this context, surface-enhanced Raman scattering (SERS) offers the promise of a simplified, rapid, and high-sensitive detection of biomolecular interactions with several advantages over previous assay methodologies. In this work, we have conceived reproducible SERS nanosensors based on tailored multilayer octupolar nanostructures which can combine high enhancement factor and remarkable molecular selectivity...
July 28, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28675248/quick-bacteriophage-mediated-bioluminescence-assay-for-detecting-staphylococcus-spp-in-sonicate-fluid-of-orthopaedic-artificial-joints
#11
Katja Šuster, Aleš Podgornik, Andrej Cör
Staphylococcus spp. accounts for up to two thirds of all microorganisms causing prosthetic joint infections, with Staphylococcus aureus and Staphylococcus epidermidis being the major cause. The present study describes a diagnostic model to detect staphylococci using a specific bacteriophage and bioluminescence detection, exploring the possibility of its use on sonicate fluid of orthopaedic artificial joints. Intracellular adenosine-5'-triphosphate release by bacteriophage mediated lysis of staphylococci was assessed to determine optimal parameters for detection...
July 2017: New Microbiologica
https://www.readbyqxmd.com/read/28643099/-beyond-antibiotic-therapy-future-antiinfective-strategies-update-2017
#12
REVIEW
D Vogt, S Sperling, T Tkhilaishvili, A Trampuz, J-P Pirnay, C Willy
BACKGROUND: The key elements in the therapy of surgical site infections (SSI) are surgical debridement and local and systemic antibiotic therapy; however, due to increasing antibiotic resistance, the development of additional therapeutic measures is of great interest for future trauma and orthopedic surgery. METHOD: Against the background of our own experimental and clinical experiences and on the basis of the current literature, possible future anti-infective strategies were elaborated...
July 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/28532790/a-safe-and-molecular-tagged-brucella-canis-ghosts-confers-protection-against-virulent-challenge-in-mice
#13
Jing Qian, Zhaoyang Bu, Xulong Lang, Guangmou Yan, Yanling Yang, Xiuran Wang, Xinglong Wang
Canine brucellosis, caused by Brucella canis, is a persistent infectious reproductive disease in dogs. The absence of effective treatment to the intracellular pathogen and the irreversible consequence of infection makes the need of a specific vaccine urgent. Bacterial ghosts (BGs) are the empty envelopes of bacteria with no genome content inside, which emerge as a proper vaccine candidate due to its intact outer antigen. It is generally derived from a genetically engineered strain, through the expression of Bacteriophage phiX174 lysis E gene upon induction...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28414631/rathayibacter-toxicus-other-rathayibacter-species-inducing-bacterial-head-blight-of-grasses-and-the-potential-for-livestock-poisonings
#14
REVIEW
Timothy D Murray, Brenda K Schroeder, William L Schneider, Douglas G Luster, Aaron Sechler, Elizabeth E Rogers, Sergei A Subbotin
Rathayibacter toxicus, a Select Agent in the United States, is one of six recognized species in the genus Rathayibacter and the best known due to its association with annual ryegrass toxicity, which occurs only in parts of Australia. The Rathayibacter species are unusual among phytopathogenic bacteria in that they are transmitted by anguinid seed gall nematodes and produce extracellular polysaccharides in infected plants resulting in bacteriosis diseases with common names such as yellow slime and bacterial head blight...
July 2017: Phytopathology
https://www.readbyqxmd.com/read/28411223/modified-bacteriophage-s16-long-tail-fiber-proteins-for-rapid-and-specific-immobilization-and-detection-of-salmonella-cells
#15
Jenna M Denyes, Matthew Dunne, Stanislava Steiner, Maximilian Mittelviefhaus, Agnes Weiss, Herbert Schmidt, Jochen Klumpp, Martin J Loessner
Bacteriophage-based assays and biosensors rival traditional antibody-based immunoassays for detection of low-level Salmonella contaminations. In this study, we harnessed the binding specificity of the long tail fiber (LTF) from bacteriophage S16 as an affinity molecule for the immobilization, enrichment, and detection of Salmonella We demonstrate that paramagnetic beads (MBs) coated with recombinant gp37-gp38 LTF complexes (LTF-MBs) are highly effective tools for rapid affinity magnetic separation and enrichment of Salmonella Within 45 min, the LTF-MBs consistently captured over 95% of Salmonella enterica serovar Typhimurium cells from suspensions containing from 10 to 10(5) CFU · ml(-1), and they yielded equivalent recovery rates (93% ± 5%, n = 10) for other Salmonella strains tested...
June 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28403327/internal-control-for-real-time-polymerase-chain-reaction-based-on-ms2-bacteriophage-for-rna-viruses-diagnostics
#16
Miriam Ribas Zambenedetti, Daniela Parada Pavoni, Andreia Cristine Dallabona, Alejandro Correa Dominguez, Celina de Oliveira Poersch, Stenio Perdigão Fragoso, Marco Aurélio Krieger
BACKGROUND: Real-time reverse transcription polymerase chain reaction (RT-PCR) is routinely used to detect viral infections. In Brazil, it is mandatory the use of nucleic acid tests to detect hepatitis C virus (HCV), hepatitis B virus and human immunodeficiency virus in blood banks because of the immunological window. The use of an internal control (IC) is necessary to differentiate the true negative results from those consequent from a failure in some step of the nucleic acid test. OBJECTIVES: The aim of this study was the construction of virus-modified particles, based on MS2 bacteriophage, to be used as IC for the diagnosis of RNA viruses...
May 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28389547/engineering-of-bacteriophages-y2-dpol1-c-and-y2-luxab-for-efficient-control-and-rapid-detection-of-the-fire-blight-pathogen-erwinia-amylovora
#17
Yannick Born, Lars Fieseler, Valentin Thöny, Nadja Leimer, Brion Duffy, Martin J Loessner
Erwinia amylovora is the causative agent of fire blight, a devastating plant disease affecting members of the Rosaceae Alternatives to antibiotics for control of fire blight symptoms and outbreaks are highly desirable, due to increasing drug resistance and tight regulatory restrictions. Moreover, the available diagnostic methods either lack sensitivity, lack speed, or are unable to discriminate between live and dead bacteria. Owing to their extreme biological specificity, bacteriophages are promising alternatives for both aims...
June 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28349164/lateral-flow-assay-for-rapid-detection-of-white-spot-syndrome-virus-wssv-using-a-phage-displayed-peptide-as-bio-recognition-probe
#18
Prabir Kumar Kulabhusan, Jyutika M Rajwade, A S Sahul Hameed, Kishore M Paknikar
White spot disease caused by the white spot syndrome virus (WSSV) has a major socio-economic impact on shrimp farming in India. It has been realized that a field-usable diagnostic capable of rapid detection of WSSV can prevent huge economic losses in disease outbreaks. In this work, we explored the possibility of using a peptide as bio-recognition probe in a field-usable device for the detection of WSSV from infected shrimps and prawns. A commercially available random phage-display library was screened against rVP28 (a major structural protein of WSSV, expressed as a recombinant protein in Escherichia coli)...
March 27, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28337580/bacteriophage-encoded-virion-associated-enzymes-to-overcome-the-carbohydrate-barriers-during-the-infection-process
#19
REVIEW
Agnieszka Latka, Barbara Maciejewska, Grazyna Majkowska-Skrobek, Yves Briers, Zuzanna Drulis-Kawa
Bacteriophages are bacterial viruses that infect the host after successful receptor recognition and adsorption to the cell surface. The irreversible adherence followed by genome material ejection into host cell cytoplasm must be preceded by the passage of diverse carbohydrate barriers such as capsule polysaccharides (CPSs), O-polysaccharide chains of lipopolysaccharide (LPS) molecules, extracellular polysaccharides (EPSs) forming biofilm matrix, and peptidoglycan (PG) layers. For that purpose, bacteriophages are equipped with various virion-associated carbohydrate active enzymes, termed polysaccharide depolymerases and lysins, that recognize, bind, and degrade the polysaccharide compounds...
April 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28331958/nucleic-acid-sensing-with-enzyme-dna-binding-protein-conjugates-cascade-and-simple-dna-nanostructures
#20
Gülsen Betül Aktas, Vasso Skouridou, Lluis Masip
A versatile and universal DNA sensing platform is presented based on enzyme-DNA binding protein tags conjugates and simple DNA nanostructures. Two enzyme conjugates were thus prepared, with horseradish peroxidase linked to the dimeric single-chain bacteriophage Cro repressor protein (HRP-scCro) and glucose oxidase linked to the dimeric headpiece domain of Escherichia coli LacI repressor protein (GOx-dHP), and used in conjunction with a hybrid ssDNA-dsDNA detection probe. This probe served as a simple DNA nanostructure allowing first for target recognition through its target-complementary single-stranded DNA (ssDNA) part and then for signal generation after conjugate binding on the double-stranded DNA (dsDNA) containing the specific binding sites for the dHP and scCro DNA binding proteins...
March 22, 2017: Analytical and Bioanalytical Chemistry
keyword
keyword
6271
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"