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https://www.readbyqxmd.com/read/28719657/isolation-and-in-vitro-evaluation-of-bacteriophages-against-mdr-bacterial-isolates-from-septic-wound-infections
#1
Roja Rani Pallavali, Vijaya Lakshmi Degati, Dakshayani Lomada, Madhava C Reddy, Vijaya Raghava Prasad Durbaka
Multi-drug resistance has become a major problem for the treatment of pathogenic bacterial infections. The use of bacteriophages is an attractive approach to overcome the problem of drug resistance in several pathogens that cause fatal diseases. Our study aimed to isolate multi drug resistant bacteria from patients with septic wounds and then isolate and apply bacteriophages in vitro as alternative therapeutic agents. Pus samples were aseptically collected from Rajiv Gandhi Institute of Medical Science (RIMS), Kadapa, A...
2017: PloS One
https://www.readbyqxmd.com/read/28719102/plant-viral-and-bacteriophage-delivery-of-nucleic-acid-therapeutics
#2
REVIEW
Patricia Lam, Nicole F Steinmetz
Nucleic acid therapeutics have emerged as a powerful method for treatment of many diseases. However, the challenge lies in safe and efficient delivery of nucleic acids to their target site, as they need to cross various extracellular and intracellular barriers. Mammalian viruses have initially been favored for delivery of nucleic acid therapeutics, but safety concerns regarding their immunogenicity and potential of integration have fueled the search for alternative delivery strategies. For example, chemistry and bioengineering have led to advances in the use of nonviral vectors composed of lipids and other polymers; nevertheless, the synthetic systems often do not match the efficiency achieved using the biological systems...
July 18, 2017: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
https://www.readbyqxmd.com/read/28717637/phage-therapy-in-a-16-year-old-boy-with-netherton-syndrome
#3
Pikria Zhvania, Naomi Sulinger Hoyle, Lia Nadareishvili, Dea Nizharadze, Mzia Kutateladze
Netherton syndrome (NS) is a rare autosomal recessive disorder, characterized by a classical triad of clinical features, including congenital ichthyosiform erythroderma, trichorrhexis invaginata, and atopic diathesis coupled with frequent bacterial infections (1). The genetic basis for the disease has been recently identified with mutations in gene SPINK5, which is involved in the regulation of formation of skin barriers. We report on a 16-year-old male with all the typical manifestations of NS, including atopic diathesis and ongoing serious staphylococcal infections and allergy to multiple antibiotics whose family sought help at the Eliava Phage Therapy Center when all other treatment options were failing...
2017: Frontiers in Medicine
https://www.readbyqxmd.com/read/28716906/virus-found-in-a-boreal-lake-links-ssdna-and-dsdna-viruses
#4
Elina Laanto, Sari Mäntynen, Luigi De Colibus, Jenni Marjakangas, Ashley Gillum, David I Stuart, Janne J Ravantti, Juha T Huiskonen, Lotta-Riina Sundberg
Viruses have impacted the biosphere in numerous ways since the dawn of life. However, the evolution, genetic, structural, and taxonomic diversity of viruses remain poorly understood, in part because sparse sampling of the virosphere has concentrated mostly on exploring the abundance and diversity of dsDNA viruses. Furthermore, viral genomes are highly diverse, and using only the current sequence-based methods for classifying viruses and studying their phylogeny is complicated. Here we describe a virus, FLiP (Flavobacterium-infecting, lipid-containing phage), with a circular ssDNA genome and an internal lipid membrane enclosed in the icosahedral capsid...
July 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28715480/tales-of-diversity-genomic-and-morphological-characteristics-of-forty-six-arthrobacter-phages
#5
Karen K Klyczek, J Alfred Bonilla, Deborah Jacobs-Sera, Tamarah L Adair, Patricia Afram, Katherine G Allen, Megan L Archambault, Rahat M Aziz, Filippa G Bagnasco, Sarah L Ball, Natalie A Barrett, Robert C Benjamin, Christopher J Blasi, Katherine Borst, Mary A Braun, Haley Broomell, Conner B Brown, Zachary S Brynell, Ashley B Bue, Sydney O Burke, William Casazza, Julia A Cautela, Kevin Chen, Nitish S Chimalakonda, Dylan Chudoff, Jade A Connor, Trevor S Cross, Kyra N Curtis, Jessica A Dahlke, Bethany M Deaton, Sarah J Degroote, Danielle M DeNigris, Katherine C DeRuff, Milan Dolan, David Dunbar, Marisa S Egan, Daniel R Evans, Abby K Fahnestock, Amal Farooq, Garrett Finn, Christopher R Fratus, Bobby L Gaffney, Rebecca A Garlena, Kelly E Garrigan, Bryan C Gibbon, Michael A Goedde, Carlos A Guerrero Bustamante, Melinda Harrison, Megan C Hartwell, Emily L Heckman, Jennifer Huang, Lee E Hughes, Kathryn M Hyduchak, Aswathi E Jacob, Machika Kaku, Allen W Karstens, Margaret A Kenna, Susheel Khetarpal, Rodney A King, Amanda L Kobokovich, Hannah Kolev, Sai A Konde, Elizabeth Kriese, Morgan E Lamey, Carter N Lantz, Jonathan S Lapin, Temiloluwa O Lawson, In Young Lee, Scott M Lee, Julia Y Lee-Soety, Emily M Lehmann, Shawn C London, A Javier Lopez, Kelly C Lynch, Catherine M Mageeney, Tetyana Martynyuk, Kevin J Mathew, Travis N Mavrich, Christopher M McDaniel, Hannah McDonald, C Joel McManus, Jessica E Medrano, Francis E Mele, Jennifer E Menninger, Sierra N Miller, Josephine E Minick, Courtney T Nabua, Caroline K Napoli, Martha Nkangabwa, Elizabeth A Oates, Cassandra T Ott, Sarah K Pellerino, William J Pinamont, Ross T Pirnie, Marie C Pizzorno, Emilee J Plautz, Welkin H Pope, Katelyn M Pruett, Gabbi Rickstrew, Patrick A Rimple, Claire A Rinehart, Kayla M Robinson, Victoria A Rose, Daniel A Russell, Amelia M Schick, Julia Schlossman, Victoria M Schneider, Chloe A Sells, Jeremy W Sieker, Morgan P Silva, Marissa M Silvi, Stephanie E Simon, Amanda K Staples, Isabelle L Steed, Emily L Stowe, Noah A Stueven, Porter T Swartz, Emma A Sweet, Abigail T Sweetman, Corrina Tender, Katrina Terry, Chrystal Thomas, Daniel S Thomas, Allison R Thompson, Lorianna Vanderveen, Rohan Varma, Hannah L Vaught, Quynh D Vo, Zachary T Vonberg, Vassie C Ware, Yasmene M Warrad, Kaitlyn E Wathen, Jonathan L Weinstein, Jacqueline F Wyper, Jakob R Yankauskas, Christine Zhang, Graham F Hatfull
The vast bacteriophage population harbors an immense reservoir of genetic information. Almost 2000 phage genomes have been sequenced from phages infecting hosts in the phylum Actinobacteria, and analysis of these genomes reveals substantial diversity, pervasive mosaicism, and novel mechanisms for phage replication and lysogeny. Here, we describe the isolation and genomic characterization of 46 phages from environmental samples at various geographic locations in the U.S. infecting a single Arthrobacter sp. strain...
2017: PloS One
https://www.readbyqxmd.com/read/28713777/a-novel-chimeric-endolysin-with-antibacterial-activity-against-methicillin-resistant-staphylococcus-aureus
#6
Hamed Haddad Kashani, Hossein Fahimi, Yasaman Dasteh Goli, Rezvan Moniri
Cysteine/histidine-dependent amidohydrolase/peptidase (CHAP) and amidase are known as catalytic domains of the bacteriophage-derived endolysin LysK and were previously reported to show lytic activity against methicillin-resistant Staphylococcus aureus (MRSA). In the current study, the in silico design and analysis of chimeric CHAP-amidase model was applied to enhance the stability and solubility of protein, which was achieved through improving the properties of primary, secondary and tertiary structures. The coding gene sequence of the chimeric CHAP-amidase was synthesized and subcloned into the pET-22(+) expression vector, and the recombinant protein was expressed in E...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28713356/a-genotypic-analysis-of-five-p-aeruginosa-strains-after-biofilm-infection-by-phages-targeting-different-cell-surface-receptors
#7
Diana P Pires, Andreas Dötsch, Erin M Anderson, Youai Hao, Cezar M Khursigara, Joseph S Lam, Sanna Sillankorva, Joana Azeredo
Antibiotic resistance constitutes one of the most serious threats to the global public health and urgently requires new and effective solutions. Bacteriophages are bacterial viruses increasingly recognized as being good alternatives to traditional antibiotic therapies. In this study, the efficacy of phages, targeting different cell receptors, against Pseudomonas aeruginosa PAO1 biofilm and planktonic cell cultures was evaluated over the course of 48 h. Although significant reductions in the number of viable cells were achieved for both cases, the high level of adaptability of the bacteria in response to the selective pressure caused by phage treatment resulted in the emergence of phage-resistant variants...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28711712/ex-vivo-and-in-vivo-evaluation-of-microemulsion-based-transdermal-delivery-of-e-coli-specific-t4-bacteriophage-a-rationale-approach-to-treat-bacterial-infection
#8
Vaibhav Rastogi, Pragya Yadav, Anurag Verma, Jayanta K Pandit
This study is focused on the development and evaluation of transdermal delivery of E. coli-specific T4 bacteriophages both ex-vivo and in-vivo using microemulsion as delivery carrier in eradicating the infection caused by E. coli. Microemulsions were prepared by mixing selected oil, surfactants and aqueous phase containing bacteriophages. The formulations were subjected to physicochemical characterization, ex-vivo and in-vivo permeation, stability studies, histological and immunofluorescence examination. The colloidal system exhibits a uniform size distribution, of finite size (150-320nm)...
July 12, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28709106/microcosms-for-evaluating-microbial-indicator-persistence-and-mobilization-in-fluvial-sediments-during-rainfall-events
#9
Julia Martín-Díaz, Cristina García-Aljaro, Míriam Pascual-Benito, Belén Galofré, Anicet R Blanch, Francisco Lucena
Mediterranean rivers, which are subject to long, dry periods and heavy rainfall events, could be particularly useful for understanding future climate scenarios. This study generated microcosms that mimicked riverbank sediment resuspension into the water of a typical Mediterranean river as a consequence of heavy rainfall. The mobilization and inactivation of six fecal pollution indicators and microbial source tracking markers were evaluated. The T90 values in the sediments were: 4 days for sorbitol-fermenting Bifidobacterium, 11 days for culturable E...
July 9, 2017: Water Research
https://www.readbyqxmd.com/read/28707382/neutron-crystallographic-studies-of-t4-lysozyme-at-cryogenic-temperature
#10
Le Li, Shantanu Shukla, Flora Meilleur, Robert F Standaert, Josh Pierce, Dean A A Myles, Matthew J Cuneo
Bacteriophage T4 lysozyme (T4L) has been used as a paradigm for seminal biophysical studies on protein structure, dynamics and stability. Approximately 700 mutants of this protein and their complexes have been characterized by X-ray crystallography; however, despite the high resolution diffraction limits attained in several studies, no hydrogen atoms were reported being visualized in the electron density maps. To address this, a 2.2 Å-resolution neutron data set was collected at 80 K from a crystal of perdeuterated T4L pseudo-wild type...
July 13, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28706995/disabling-cas9-by-an-anti-crispr-dna-mimic
#11
Jiyung Shin, Fuguo Jiang, Jun-Jie Liu, Nicolas L Bray, Benjamin J Rauch, Seung Hyun Baik, Eva Nogales, Joseph Bondy-Denomy, Jacob E Corn, Jennifer A Doudna
CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 gene editing technology is derived from a microbial adaptive immune system, where bacteriophages are often the intended target. Natural inhibitors of CRISPR-Cas9 enable phages to evade immunity and show promise in controlling Cas9-mediated gene editing in human cells. However, the mechanism of CRISPR-Cas9 inhibition is not known, and the potential applications for Cas9 inhibitor proteins in mammalian cells have not been fully established...
July 2017: Science Advances
https://www.readbyqxmd.com/read/28705962/genome-sequences-of-five-streptomyces-bacteriophages-forming-cluster-bg
#12
Richard Donegan-Quick, Zane A Gibbs, Patricia O Amaku, Joshua T Bernal, Dana A M Boyd, Angela R Burr, Rainna E Coelho, Akpedje S Dossou, Ryan M Henry, Mai Huynh, Thalia A Kanani-Hendijani, Guillermina Martinez, Tianeaka O McClendon-Moss, Sergio Orozco, Johnny M San Martin, Kaitlyn E Stoddart, Madison M Stringer, Rachel L Villegas, Subhayu Nayek, Nikita Suri, Rebecca A Garlena, Daniel A Russell, Lee E Hughes
Cluster BG of the actinobacteriophage was formed upon discovery of five novel bacteriophages isolated by enrichment from their host, Streptomyces griseus subsp. griseus strain ATCC 10137. Four members of this cluster (BabyGotBac, Maih, TP1605, and YDN12) share over 89% average nucleotide identity, while the other (Xkcd426) has only 72% similarity to other cluster members.
July 13, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28705961/draft-genome-sequence-of-bacteriophage-vb_eco_swan01
#13
Slawomir Michniewski, Tamsin Redgwell, David J Scanlan, Andrew D Millard
Bacteriophage vB_Eco_swan01 was isolated from an ornamental pool using Escherichia coli MG1655 as the host. Bacteriophage vB_Eco_swan01 has limited similarity with other known phages at the nucleotide level and likely represents a new bacteriophage species within the Tunavirinae.
July 13, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28705796/tracking-adventitial-fibroblast-contribution-to-disease-a-review-of-current-methods-to-identify-resident-fibroblasts
#14
REVIEW
Jill T Kuwabara, Michelle D Tallquist
Cells present in the adventitia, or outermost layer of the blood vessel, contribute to the progression of vascular diseases, such as atherosclerosis, hypertension, and aortic dissection. The adventitial fibroblast of the aorta is the prototypic perivascular fibroblast, but the adventitia is composed of multiple distinct cell populations. Therefore, methods for uniquely identifying the fibroblast are critical for a better understanding of how these cells contribute to disease processes. A popular method for distinguishing adventitial cell types relies on the use of genetic tools in the mouse to trace and manipulate these cells...
July 13, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28705762/flexible-connectors-between-capsomer-subunits-that-regulate-capsid-assembly
#15
Mary L Hasek, Joshua B Maurer, Roger W Hendrix, Robert L Duda
Viruses build icosahedral capsids of specific size and shape by regulating the spatial arrangement of the hexameric and pentameric protein capsomers in the growing shell during assembly. In the T=7 capsids of E. coli bacteriophage HK97 and other phages, sixty capsomers are hexons, while the rest are pentons that are correctly positioned during assembly. Assembly of the HK97 capsid to the correct size and shape has been shown to depend on specific ionic contacts between capsomers. We now describe additional ionic interactions within capsomers that also regulate assembly...
July 10, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28705159/an-att-site-based-recombination-reporter-system-for-genome-engineering-and-synthetic-dna-assembly
#16
Michael J Bland, Magaly Ducos-Galand, Marie-Eve Val, Didier Mazel
BACKGROUND: Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable directional exchange and relocation of DNA sequences, making them valuable tools in these contexts. We have developed site-specific recombination tools that allow the direct selection of recombination events by embedding the attB site from each system within the β-lactamase resistance coding sequence (bla)...
July 14, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28704651/synergy-between-the-host-immune-system-and-bacteriophage-is-essential-for-successful-phage-therapy-against-an-acute-respiratory-pathogen
#17
Dwayne R Roach, Chung Yin Leung, Marine Henry, Eric Morello, Devika Singh, James P Di Santo, Joshua S Weitz, Laurent Debarbieux
The rise of multi-drug-resistant (MDR) bacteria has spurred renewed interest in the use of bacteriophages in therapy. However, mechanisms contributing to phage-mediated bacterial clearance in an animal host remain unclear. We investigated the effects of host immunity on the efficacy of phage therapy for acute pneumonia caused by MDR Pseudomonas aeruginosa in a mouse model. Comparing efficacies of phage-curative and prophylactic treatments in healthy immunocompetent, MyD88-deficient, lymphocyte-deficient, and neutrophil-depleted murine hosts revealed that neutrophil-phage synergy is essential for the resolution of pneumonia...
July 12, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28704569/a-virulence-associated-filamentous-bacteriophage-of-neisseria-meningitidis-increases-host-cell-colonisation
#18
Emmanuelle Bille, Julie Meyer, Anne Jamet, Daniel Euphrasie, Jean-Philippe Barnier, Terry Brissac, Anna Larsen, Philippe Pelissier, Xavier Nassif
Neisseria meningitidis is a commensal of human nasopharynx. In some circumstances, this bacteria can invade the bloodstream and, after crossing the blood brain barrier, the meninges. A filamentous phage, designated MDAΦ for Meningococcal Disease Associated, has been associated with invasive disease. In this work we show that the prophage is not associated with a higher virulence during the bloodstream phase of the disease. However, looking at the interaction of N. meningitidis with epithelial cells, a step essential for colonization of the nasopharynx, we demonstrate that the presence of the prophage, via the production of viruses, increases colonization of encapsulated meningococci onto monolayers of epithelial cells...
July 13, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28702648/modular-interior-loading-and-exterior-decoration-of-a-virus-like-particle
#19
Jhanvi Sharma, Masaki Uchida, Heini M Miettinen, Trevor Douglas
Virus-like particles (VLPs) derived from the bacteriophage P22 offer an interesting and malleable platform for encapsulation and multivalent presentation of cargo molecules. The packaging of cargo in P22 VLP is typically achieved through genetically enabled directed in vivo encapsulation. However, this approach does not allow control over the packing density and composition of the encapsulated cargos. Here, we have adopted an in vitro assembly approach to gain control over cargo packaging in P22. The packaging was controlled by closely regulating the stoichiometric ratio of cargo-fused-scaffold protein and wild-type scaffold protein during the in vitro assembly...
July 12, 2017: Nanoscale
https://www.readbyqxmd.com/read/28702137/phage-therapy-assessment-of-the-efficacy-of-a-bacteriophage-isolated-in-the-treatment-of-salmonellosis-induced-by-salmonella-enteritidis-in-mice
#20
Farhad Nikkhahi, Mohammad Mehdi Soltan Dallal, Mahmood Alimohammadi, Abbas Rahimi Foroushani, Zahra Rajabi, Fatemeh Fardsanei, Seyed Mostafa Imeni, Parisa Torabi Bonab
AIM: This work aims to isolate and perform comparative studies of a phages active against a Salmonella enteritidis strain from Iran. Also, suitable phage candidates for therapy of mice will be selected. BACKGROUND: Bacteriophage is of particular interest as a biocontrol agent in the prevention of food-borne illnesses. In recent years tend to use bacteriophages to control pathogenic bacteria has increased. A bacteriophage is considered to be a potent antibiotic alternative for treating bacterial infections...
2017: Gastroenterology and Hepatology From Bed to Bench
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