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https://www.readbyqxmd.com/read/29656182/co-culture-of-ovarian-cancer-stem-like-cells-with-macrophages-induced-skov3-cells-stemness-via-il-8-stat3-signaling
#1
Yingxia Ning, Yinghong Cui, Xiang Li, Xiaocheng Cao, A Chen, Chang Xu, Jianguo Cao, Xin Luo
Among recent concepts in the cancer biology field, the tumor microenvironment is highly associated with cancer stem cells, and plays a key role in tumor progression. This study aimed to explore the mechanism that the stemness induction of SKOV3 cell line by macrophages derived from THP-1 cells, which was co-cultured with SKOV3-derived ovarian cancer stem-like cells (OCSLCs). Sphere formation, soft agar colony formation, and expression levels of CD133 and CD44 were assessed to reflect OCSLC properties. ELISA was used to evaluate secretion profile changes in macrophages co-cultured with or without SKOV3-derived OCSLCs...
April 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29655787/secretory-expression-and-scale-up-production-of-recombinant-human-thyroid-peroxidase-via-baculovirus-insect-cell-system-in-a-wave-type-bioreactor
#2
Ying Lou, Guorong Ji, Qin Liu, Pengbo Wang, Ruilin Zhang, Yuanxing Zhang, Xiaohong Liu
The human thyroid peroxidase (hTPO) is an essential enzyme for thyroid hormone biosynthesis and is expressed in thyroid cells. It is an autoantigen against which antibodies are found in the sera of patients with a number of autoimmune thyroid disorders. Overexpression of hTPO has been achieved using the baculovirus expression vector system (BEVS). However, it is produced largely in an aggregated form in the cell lysate fraction, which increases the complexity of protein extraction. In this study, to achieve improved secretory expression of hTPO via BEVS, a truncated recombinant hTPO protein (hTPOt) was engineered by replacing its original signal peptide (SP) in the N-terminal with five heterologous SPs...
April 12, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29649688/recombinant-outer-membrane-protein-25c-from-brucella-abortus-induces-th1-and-th2-mediated-protection-against-brucella-abortus-infection-in-mouse-model
#3
Soumya Paul, Bhavani V Peddayelachagiri, Sowmya Nagaraj, Joseph J Kingston, Harsh V Batra
Development of a safe and efficacious vaccine for brucellosis is a long standing challenge for scientists. Recognizing potential antigens towards developing vaccine candidate is crucial. Omp25c, a porin protein of Brucella, is a paralog of two previously identified promising vaccine candidates namely, Omp25 and Omp31, with notable sequence identity. Also, Omp25c is conserved in all major Brucella species. This highlights the possibility of employing this protein in multivalent subunit vaccine based approach of Brucella management...
April 9, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29648589/analysis-of-the-3h8-antigen-of-candidaalbicans-reveals-new-aspects-of-the-organization-of-fungal-cell-wall-proteins
#4
Rafael Sentandreu, Antonio Caminero, Itzel Rentería, Claudia León-Ramirez, Luis González-de-la-Vara, Eulogio Valentin-Gomez, José Ruiz-Herrera
The walls of both, yeast and mycelial cells of Candida albicans possess a species specific antigen that is recognized by a monoclonal antibody (MAb 3H8). This antigen can be extracted in the form of a very high Mr complex, close or over 106 Da, by treatment, with β-1,3 glucanase, β mercaptoethanol or dithothreitol, or mild alkali, but not by saturated HF in pyridine, suggesting that the complex is bound to wall β-1,3 glucans, and to proteins by disulfide bonds, but not to β-1,6 glucans. Through its sensitivity to trypsin and different deglycosylation procedures, it was concluded that the epitope is associated to a glycoprotein containing N-glycosidic, but not O-glycosidic mannan moieties...
April 10, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29643819/hmgb1-increases-il-1%C3%AE-production-in-vascular-smooth-muscle-cells-via-nlrp3-inflammasome
#5
Eun Jung Kim, So Youn Park, Seung Eun Baek, Min A Jang, Won Suk Lee, Sun Sik Bae, Koanhoi Kim, Chi Dae Kim
Vascular smooth muscle cells (VSMCs) are the major cell type in the blood vessel walls, and their phenotypic modulation is a key cellular event driving vascular remodeling. Although high mobility group box-1 (HMGB1) plays a pivotal role in inflammatory processes after vascular injuries, the importance of the links between VSMCs, HMGB1 and vascular inflammation has not been clarified. To prove the hypothesis that VSMCs might be active players in vascular inflammation by secreting inflammatory cytokines, we investigated the proinflammatory effects of HMGB1 and its intermediary signaling pathways in VSMCs...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29643357/release-of-staphylococcus-aureus-extracellular-vesicles-and-their-application-as-a-vaccine-platform
#6
Xiaogang Wang, Christopher D Thompson, Christopher Weidenmaier, Jean C Lee
Secretion of extracellular vesicles (EVs), a process common to eukaryotes, archae, and bacteria, represents a secretory pathway that allows cell-free intercellular communication. Microbial EVs package diverse proteins and influence the host-pathogen interaction, but the mechanisms underlying EV production in Gram-positive bacteria are poorly understood. Here we show that EVs purified from community-associated methicillin-resistant Staphylococcus aureus package cytosolic, surface, and secreted proteins, including cytolysins...
April 11, 2018: Nature Communications
https://www.readbyqxmd.com/read/29629941/b-cells-in-multiple-sclerosis
#7
Mireia Sospedra
PURPOSE OF REVIEW: Although it is becoming increasingly clear that B cells play important roles in multiple sclerosis (MS) pathogenesis, it is incompletely understood how they contribute. The purpose of this review is to provide an overview of the current knowledge about B cells in MS taking into account MS heterogeneity. RECENT FINDINGS: The efficacy of B cell-depleting therapies has provided strong evidence for the involvement of these cells in MS pathogenesis...
April 7, 2018: Current Opinion in Neurology
https://www.readbyqxmd.com/read/29627497/osteoblast-secreted-wisp-1-promotes-adherence-of-prostate-cancer-cells-to-bone-via-the-vcam-1-integrin-%C3%AE-4%C3%AE-1-system
#8
An-Chen Chang, Po-Chun Chen, Yu-Feng Lin, Chen-Ming Su, Ju-Fang Liu, Tien-Huang Lin, Show-Mei Chuang, Chih-Hsin Tang
Bone metastasis is a frequent occurrence in prostate cancer (PCa) that is associated with severe complications such as fracture, bone pain and hypercalcemia. The cross-talk between metastatic cancer cells and bone is critical to the development and progression of bone metastases. In our previous data, we have described how the involvement of the Wnt-induced secreted protein-1/vascular cell adhesion molecule-1 (WISP-1/VCAM-1) system in this tumor-bone interaction contributes to human PCa cell motility. In this study, we found that WISP-1 regulates bone mineralization by inducing bone morphogenetic protein-2 (BMP2), BMP4 and osteopontin (OPN) expression in osteoblasts...
April 5, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29626211/targeting-cytokines-to-treat-asthma-and-chronic-obstructive-pulmonary-disease
#9
REVIEW
Peter J Barnes
Cytokines play a key role in orchestrating and perpetuating the chronic airway inflammation in asthma and chronic obstructive pulmonary disease (COPD), making them attractive targets for treating these disorders. Asthma and some cases of COPD are mainly driven by type 2 immune responses, which comprise increased airway eosinophils, T helper 2 (TH 2) cells and group 2 innate lymphoid cells (ILC2s) and the secretion of IL-4, IL-5 and IL-13. Clinical trials of antibodies that block these interleukins have shown reduced acute exacerbations and oral corticosteroid use and improvements in lung function and symptoms in selected patients...
April 6, 2018: Nature Reviews. Immunology
https://www.readbyqxmd.com/read/29616672/erythrocyte-phenotype-in-a-pregnant-woman-of-sri-lanka-description-of-the-case-and-complications-related-to-communication-problems
#10
Alvaro Ringressi, Veronica Cunsolo, Francesca Malentacchi, Simone Pozzessere
BACKGROUND: The Bombay phenotype is a rare genetic trait which is characterized by the absence of A, B and H antigens on red cells as well as in body secretions. The serum shows the presence of antibodies against antigen H. Patients with this rare blood type are not easily transfusable. We had observed a woman aged 18, at the 20th week of pregnancy, native of Sri Lanka, with an IgG and IgM class anti-H. We report the case and the clinical issues arisen. MATERIALS AND METHODS: The determination of ABO, Rh[D] group, the indirect antiglobulin test (IAT) were performed in tube techniques and in neutral gel microcolumn...
January 2018: Annali Dell'Istituto Superiore di Sanità
https://www.readbyqxmd.com/read/29616019/the-syk-coupled-c-type-lectin-receptors-dectin-2-and-dectin-3-are-involved-in-paracoccidioides-brasiliensis-recognition-by-human-plasmacytoid-dendritic-cells
#11
Nycolas Willian Preite, Claudia Feriotti, Dhêmerson Souza de Lima, Bruno Borges da Silva, Antônio Condino-Neto, Alessandra Pontillo, Vera Lúcia Garcia Calich, Flávio Vieira Loures
Plasmacytoid dendritic cells (pDCs), which have been extensively studied in the context of the immune response to viruses, have recently been implicated in host defense mechanisms against fungal infections. Nevertheless, the involvement of human pDCs during paracoccidioidomycosis (PCM), a fungal infection endemic to Latin America, has been scarcely studied. However, pDCs were found in the cutaneous lesions of PCM patients, and in pulmonary model of murine PCM these cells were shown to control disease severity...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29615881/age-dependent-hypothalamic-and-pituitary-responses-to-novel-environment-stress-or-lipopolysaccharide-in-rats
#12
Sandy Koenig, Janne Bredehöft, Alexander Perniss, Franziska Fuchs, Joachim Roth, Christoph Rummel
Previously, we have shown that the transcription factor nuclear factor interleukin (NF-IL)6 can be used as an activation marker for inflammatory lipopolysaccharide (LPS)-induced and psychological novel environment stress (NES) in the rat brain. Here, we aimed to investigate age dependent changes of hypothalamic and pituitary responses to NES (cage switch) or LPS (100 μg/kg) in 2 and 24 months old rats. Animals were sacrificed at specific time points, blood and brains withdrawn and analyzed using immunohistochemistry, RT-PCR and bioassays...
2018: Frontiers in Behavioral Neuroscience
https://www.readbyqxmd.com/read/29615689/involvement-of-rsk1-activation-in-malformin-enhanced-cellular-fibrinolytic-activity
#13
Yukio Koizumi, Kenichiro Nagai, Lina Gao, Souichi Koyota, Tomokazu Yamaguchi, Miyuki Natsui, Yumiko Imai, Keiji Hasumi, Toshihiro Sugiyama, Keiji Kuba
Pharmacological interventions to enhance fibrinolysis are effective for treating thrombotic disorders. Utilizing the in vitro U937 cell line-based fibrin degradation assay, we had previously found a cyclic pentapeptide malformin A1 (MA1 ) as a novel activating compound for cellular fibrinolytic activity. The mechanism by which MA1 enhances cellular fibrinolytic activity remains unknown. In the present study, we show that RSK1 is a crucial mediator of MA1 -induced cellular fibrinolysis. Treatment with rhodamine-conjugated MA1 showed that MA1 localizes mainly in the cytoplasm of U937 cells...
April 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29614511/tumor-derived-exosomal-long-noncoding-rnas-as-promising-diagnostic-biomarkers-for-prostate-cancer
#14
Yu-Hui Wang, Jia Ji, Bi-Cheng Wang, Hao Chen, Zhong-Hua Yang, Kun Wang, Chang-Liang Luo, Wu-Wen Zhang, Fu-Bing Wang, Xiao-Lian Zhang
BACKGROUND/AIMS: Exosomal circulating long non-coding RNAs (lncRNAs) in blood are emerging as clinically useful and non-invasive biomarkers for tumor diagnosis. However, normal cells can also secrete exosomes, so it is a prerequisite to obtain tumor-derived exosomes for better understanding of their diagnostic impacts in cancer. In this study, a dual-antibody-functionalized immunoaffinity system was established to isolate exosomes and investigate their lncRNAs expression pattern and clinical significance in prostate cancer (PCa)...
March 26, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29613835/therapeutic-exploitation-of-ipse-a-urogenital-parasite-derived-host-modulatory-protein-for-chemotherapy-induced-hemorrhagic-cystitis
#15
Evaristus C Mbanefo, Loc Le, Luke F Pennington, Justin I Odegaard, Theodore S Jardetzky, Abdulaziz Alouffi, Franco H Falcone, Michael H Hsieh
Chemotherapy-induced hemorrhagic cystitis (CHC) can be difficult to manage. Prior work suggests that IL-4 alleviates ifosfamide-induced hemorrhagic cystitis (IHC), but systemically administered IL-4 causes significant side effects. We hypothesized that the Schistosoma hematobium homolog of IL-4-inducing principle from Schistosoma mansoni eggs (H-IPSE), would reduce IHC and associated bladder pathology. IPSE binds IgE on basophils and mast cells, triggering IL-4 secretion by these cells. IPSE is also an "infiltrin," translocating into the host nucleus to modulate gene transcription...
April 3, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29610847/the-imbalance-of-lymphocyte-subsets-and-cytokines-potential-immunologic-insights-into-the-pathogenesis-of-chronic-dacryocystitis
#16
Xiaoxuan Yang, Lizhen Wang, Lunhao Li, Zhang Yu, Caiwen Xiao
Purpose: To explore the quantitative distributions of different lymphocyte subsets in the lacrimal sac mucosa and identify changes of Th1- and Th2-associated cytokines in the tears of patients with chronic dacryocystitis. Methods: Lacrimal sac mucosal specimens from patients with chronic dacryocystitis were analyzed. Hematoxylin-eosin staining and Masson staining were performed for pathologic analysis, and immunohistochemical staining was performed for the detection of CD3+, CD4+, CD8+, CD20+, Th1, and Th2 lymphocytes...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29610041/fumarate-hydratase-specific-t-cell-response-in-chinese-patients-with-autoimmune-hepatitis
#17
Yan Zhao, Yanli Li, Dantong Zhao, Haiping Zhang, Yanmin Liu, Huiyu Liao, Yonghong Zhang, Huiping Yan
PURPOSE: Fumarate hydratase (FH) is expressed in the serum of patients with autoimmune hepatitis (AIH). The specific involvement of FH-specific T cell response is currently unknown. The aim of the study was to assess the frequency and clinical significance of FH-specific T cell response in AIH. METHODS: This was a prospective study of 42 consecutive patients admitted to the clinical study center of autoimmune liver disease of our Hospital, Capital Medical University (China) between January 2011 and December 2014...
March 30, 2018: Clinics and Research in Hepatology and Gastroenterology
https://www.readbyqxmd.com/read/29608951/aspergillus-specific-antibodies-targets-and-applications
#18
REVIEW
Max Schubert, Holger Spiegel, Stefan Schillberg, Greta Nölke
Aspergillus is a fungal genus comprising several hundred species, many of which can damage the health of plants, animals and humans by direct infection and/or due to the production of toxic secondary metabolites known as mycotoxins. Aspergillus-specific antibodies have been generated against polypeptides, polysaccharides and secondary metabolites found in the cell wall or secretions, and these can be used to detect and monitor infections or to quantify mycotoxin contamination in food and feed. However, most Aspergillus-specific antibodies are generated against heterogeneous antigen preparations and the specific target remains unknown...
March 30, 2018: Biotechnology Advances
https://www.readbyqxmd.com/read/29606863/cigarette-smoke-exposure-promotes-differentiation-of-cd4-t-cells-toward-th17-cells-by-cd40-cd40l-costimulatory-pathway-in-mice
#19
Yi Liang, Ying Shen, Liangjian Kuang, Guang Zhou, Longju Zhang, Xiaoning Zhong, Jianquan Zhang, Jifeng Liu
Purpose: This study aimed to investigate the impact of cigarette smoke exposure upon CD40-CD40L ligation between bone marrow-derived dendritic cells (BMDCs)and CD4+ T cells, and to examine the effects of cigarette smoke exposure upon differentiation of CD4+ T cells toward Th17 cells through blockade of CD40-CD40L pathway in mice. Methods: The study was processed in vivo and in vitro. In vivo, Th17 cells, CD40, interleukin (IL)-17A, and IL-27 in the lung tissues were quantified and compared between mice with and without cigarette smoke exposure...
2018: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/29604293/immune-cell-production-of-interleukin-17-induces-stem-cell-features-of-pancreatic-intraepithelial-neoplasia-cells
#20
Yu Zhang, Michelle Zoltan, Erick Riquelme, Hanwen Xu, Ismet Sahin, Susana Castro-Pando, Maria Fernanda Montiel, Kyle Chang, Zhengyu Jiang, Jianhua Ling, Sonal Gupta, William Horne, Melissa Pruski, Huamin Wang, Shao-Cong Sun, Guillermina Lozano, Paul Chiao, Anirban Maitra, Steven D Leach, Jay K Kolls, Eduardo Vilar Sanchez, Timothy C Wang, Jennifer M Bailey, Florencia McAllister
BACKGROUND & AIMS: Little is known about how the immune system affects stem cell features of pancreatic cancer cells. Immune cells that produce interleukin 17 A (IL17A) in the chronically inflamed pancreas (chronic pancreatitis) contribute to PanIN initiation and progression. We investigated the effects of IL17A signaling exerts on pancreatic cancer progenitor cells and the clinical relevance of this phenomena. METHODS: We performed studies with Mist1Cre;LSLKras;Rosa26mTmG (KCiMist ;G) and Kras(G12D); Trp53(R172H); Pdx1-Cre (KPC) mice (which upon tamoxifen induction spontaneously develop pancreatic intraepithelial neoplasias, PanINs) and control littermates...
March 28, 2018: Gastroenterology
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