Xinting Yang, Jun Yan, Yu Xue, Qing Sun, Yun Zhang, Ru Guo, Chaohong Wang, Xuelian Li, Qingtao Liang, Hangyu Wu, Chong Wang, Xinlei Liao, Sibo Long, Maike Zheng, Rongrong Wei, Haoran Zhang, Yi Liu, Nanying Che, Laurence Don Wai Luu, Junhua Pan, Guirong Wang, Yi Wang
BACKGROUND: Tuberculosis (TB) is caused by Mycobacterium tuberculosis ( Mtb ) and remains a major health threat worldwide. However, a detailed understanding of the immune cells and inflammatory mediators in Mtb -infected tissues is still lacking. Tuberculous pleural effusion (TPE), which is characterized by an influx of immune cells to the pleural space, is thus a suitable platform for dissecting complex tissue responses to Mtb infection. METHODS: We employed singe-cell RNA sequencing to 10 pleural fluid (PF) samples from 6 patients with TPE and 4 non-TPEs including 2 samples from patients with TSPE (transudative pleural effusion) and 2 samples with MPE (malignant pleural effusion)...
2023: Frontiers in Immunology