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https://www.readbyqxmd.com/read/29288041/global-quality-assessment-of-liver-allograft-c4d-staining-during-acute-antibody-mediated-rejection-in-formalin-fixed-paraffin-embedded-tissue
#1
Neil Dah, Bellamy Co, M Smith, H Haga, Y Zen, M Sebagh, K Ruppert, J Lunz, S G Hübscher, A J Demetris
Discussion of liver antibody mediated rejection during the 2011, 2013 and 2015 Banff liver sessions raised concerns over reliability of complement fragment 4d (C4d) staining, precipitating a global survey followed by a tissue microarray staining quality assessment study among centers on formalin-fixed, paraffin-embedded tissue. Tissue microarray sections containing tissue plugs of resected native and allograft (with acute antibody mediated rejection) liver, heart and kidney (n=33 total cores) were sent to 31 centers for C4d staining using local method (s) and pathologist scoring...
December 26, 2017: Human Pathology
https://www.readbyqxmd.com/read/29235086/endothelial-expression-of-c-kit-and-cd68-in-dental-follicles-of-human-impacted-third-molars
#2
Mugurel Constantin Rusu, Aliona Rașcu, Mihai Dragomir Stoenescu
Periodontal tissue remnants of odontogenesis constitute the dental follicle (DF) which is actually considered a stem niche in adults. However, potentialities of local endothelia within this niche seem overlooked. We thus aimed at testing the endothelial cells expression of c-kit, the progenitor cells marker, and CD68, commonly regarded as a monocyte/macrophage marker, in human DFs. We performed an immunohistochemical study using these two markers which were applied on samples collected from ten adult patients...
December 13, 2017: Folia Morphologica (Warsz)
https://www.readbyqxmd.com/read/29220293/pathologic-angiogenesis-of-malignant-vascular-sarcomas-implications-for-treatment
#3
Jalal A Khan, Robert G Maki, Vinod Ravi
Angiosarcoma, epithelioid hemangioendothelioma, and Kaposi sarcoma are classified according to the line of differentiation that these neoplastic cells most closely resemble: the endothelial cell. Although these malignant vascular sarcomas demonstrate immunohistochemical and ultrastructural features typical of this lineage, they vary dramatically in presentation and behavior, reflecting oncologic mechanisms unique to each. Antineoplastic therapies offer significant benefit, but because of the rarity of these cancers, novel therapies are slow to develop, and treatment options for these cancers remain limited...
December 8, 2017: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/29196060/mir-365-promotes-diabetic-retinopathy-through-inhibiting-timp3-and-increasing-oxidative-stress
#4
Juan Wang, Jieping Zhang, Xin Chen, Yiting Yang, Fang Wang, Weiye Li, Maihemuti Awuti, Yaping Sun, Chunpin Lian, Zongyi Li, Min Wang, Jing-Ying Xu, Caixia Jin, Haibin Tian, Furong Gao, Jingfa Zhang, Debasish Sinha, Lixia Lu, Guo-Tong Xu
miRs play critical roles in oxidative stress-related retinopathy pathogenesis. miR-365 was identified in a previously constructed library from glyoxal-treated rat Müller cell. This report explores epigenetic alterations in Müller cells under oxidative stress to develop a novel therapeutic strategy. To examine the miR-365 expression pattern, in situ hybridization and quantitative RT-PCR were performed. Bioinformatical analysis and dual luciferase report assay were applied to identify and confirm target genes...
November 28, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29192323/significant-inhibition-of-infantile-hemangioma-growth-by-sustained-delivery-of-urea-from-liposomes-in-microspheres
#5
Xiaoshuang Zhu, Xiaonan Guo, Dakan Liu, Yubin Gong, Jin Sun, Changxian Dong
Infantile hemangioma (IH) is a benign pediatric tumor, and rapid growth of IH can result in serious morbidity and even mortality. Only one drug Hemangeol™ (propranolol hydrochloride oral solution) has been approved for the treatment of IH, whereas patients suffer from its adverse effects and high frequency of administration. We have used urea, an organic compound and a normal body metabolite, in the treatment of IH for 20 years, and demonstrated that urea is an effective and well-tolerated treatment for IH...
January 2018: Oncology Reports
https://www.readbyqxmd.com/read/29190776/large-scale-identification-of-human-cerebrovascular-proteins-inter-tissue-and-intracerebral-vascular-protein-diversity
#6
Soo Jung Lee, Soonhyung Kwon, John R Gatti, Ejona Korcari, Ty E Gresser, Princess C Felix, Simon G Keep, Kevin C Pasquale, Tongxu Bai, Sabrina A Blanchett-Anderson, Nancy W Wu, Charissa Obeng-Nyarko, Kossi M Senagbe, Kathy C Young, Snehaa Maripudi, Bharath C Yalavarthi, Dajana Korcari, Andre Y Liu, Benjamin C Schaffler, Richard F Keep, Michael M Wang
The human cerebrovascular system is responsible for regulating demand-dependent perfusion and maintaining the blood-brain barrier (BBB). In addition, defects in the human cerebrovasculature lead to stroke, intracerebral hemorrhage, vascular malformations, and vascular cognitive impairment. The objective of this study was to discover new proteins of the human cerebrovascular system using expression data from the Human Protein Atlas, a large-scale project which allows public access to immunohistochemical analysis of human tissues...
2017: PloS One
https://www.readbyqxmd.com/read/29183683/cellular-diversity-of-the-somatosensory-cortical-map-plasticity
#7
REVIEW
Koen Kole, Wim Scheenen, Paul Tiesinga, Tansu Celikel
Sensory maps are representations of the sensory epithelia in the brain. Despite the intuitive explanatory power behind sensory maps as being neuronal precursors to sensory perception, and sensory cortical plasticity as a neural correlate of perceptual learning, molecular mechanisms that regulate map plasticity are not well understood. Here we perform a meta-analysis of transcriptional and translational changes during altered whisker use to nominate the major molecular correlates of experience-dependent map plasticity in the barrel cortex...
November 25, 2017: Neuroscience and Biobehavioral Reviews
https://www.readbyqxmd.com/read/29180874/expression-and-significance-of-b7-h3-and-tie-2-in-the-tumor-vasculature-of-clear-cell-renal-carcinoma
#8
Xianyun Zhang, Jindong Ji, Guangbo Zhang, Chuntao Fang, Fujin Jiang, Song Ma, Jianquan Hou
Tumor angiogenesis is required for tumor growth and metastasis, and the Ang/Tie-2 axis plays a pivotal role in angiogenesis. B7-H3, a new member of the B7 family of costimulatory molecules, has a critical function in the T-cell-mediated antitumor immune response, and abnormal tumor B7-H3 expression is frequently associated with a poor prognosis. However, the relationship between B7-H3 and angiogenesis in clear cell renal carcinoma (ccRCC) remains unclear. In this study, we used immunohistochemical methods to detect tumor vascular expression of B7-H3 and Tie-2 in tissue microarrays of 82 ccRCC patient samples...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29129665/intravasation-of-sw620-colon-cancer-cell-spheroids-through-the-blood-endothelial-barrier-is-inhibited-by-clinical-drugs-and-flavonoids-in-vitro
#9
Silvio Holzner, Stefan Brenner, Atanas Georgiev Atanasov, Daniel Senfter, Serena Stadler, Chi Huu Nguyen, Adryan Fristiohady, Daniela Milovanovic, Nicole Huttary, Sigurd Krieger, Zsuzsanna Bago-Horvath, Oliver de Wever, Ioannis Tentes, Ali Özmen, Walter Jäger, Helmut Dolznig, Verena Maria Dirsch, Robert Michael Mader, Liselotte Krenn, Georg Krupitza
Mechanisms how colorectal cancer (CRC) cells penetrate blood micro-vessel endothelia and metastasise is poorly understood. To study blood endothelial cell (BEC) barrier breaching by CRC emboli, an in vitro assay measuring BEC-free areas underneath SW620 cell spheroids, so called "circular chemorepellent induced defects" (CCIDs, appearing in consequence of endothelial retraction), was adapted and supported by Western blotting, EIA-, EROD- and luciferase reporter assays. Inhibition of ALOX12 or NF-κB in SW620 cells or BECs, respectively, caused attenuation of CCIDs...
November 10, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29119310/on-the-regional-variability-of-averaged-cell-area-estimates-for-the-human-corneal-endothelium-in-relation-to-the-extent-of-polymegethism
#10
M J Doughty
PURPOSE: To assess variability in the coefficient of variation (COV) in cell area estimates when using different numbers of cells for endothelial morphometry. METHODS: Using non-contact specular microscopy images of the corneal endothelium, 4 sets of 20 cases were selected that included 200 cells and had overall (global) COV values of less than 30 (group 1), 31-40 (group 2), 41-50 (group 3) and over 50% (group 4). Subjects could be normal, or had ophthalmic disease (such as diabetes), a history of rigid or soft contact lens wear or were assessed after cataract surgery...
November 7, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/29112590/effect-of-andrographolide-on-gene-expression-profile-and-intracellular-calcium-in-primary-rat-myocardium-microvascular-endothelial-cells
#11
Bo Feng, Qian Zhang, Xin Wang, Xiangwan Sun, Xiang Mu, Hong Dong
Andrographolide (ANDRO) is a diterpene lactone compound with extensive biological effects, such as antibacterial, antitumor and treatment of cardiovascular diseases. Until now, studies on the pharmacological functions of ANDRO are still in progress. However, little is known about the gene expression profile and calcium response of endothelial cells to ANDRO. In this study, we used a microarray technology to investigate the gene expression responses in primary rat myocardium microvascular endothelial cells (RMMECs) treated with 10μg/mL ANDRO...
October 31, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/29111993/enhanced-osteogenic-differentiation-potential-of-stem-cell-spheroids-created-from-a-coculture-of-stem-cells-and-endothelial-cells
#12
Jae-Yong Tae, Sung-Il Lee, Youngkyung Ko, Jun-Beom Park
PURPOSE: This study was performed to fabricate stem-cell spheroids formed with human gingiva-derived stem cells and endothelial cells and to evaluate their viability and osteogenic differentiation potential. MATERIALS AND METHODS: Gingiva-derived stem cells were isolated, and stem cells and endothelial cells with a total of 6 × 10 cells were seeded into concave micromolds with different ratios of 6:0 (group 1), 4:2 (group 2), 3:3 (group 3), and 2:4 (group 4). RESULTS: Gingiva-derived stem cells and/or endothelia cells formed spheroids in concave microwells...
November 2, 2017: Implant Dentistry
https://www.readbyqxmd.com/read/29111372/transmural-capillary-ingrowth-is-essential-for-confluent-vascular-graft-healing
#13
Timothy Pennel, Deon Bezuidenhout, Josepha Koehne, Neil Davies, Peter Zilla
Spontaneous endothelialization of synthetic vascular grafts may occur via three independent or concurrent modalities: transanastomotic (TA) outgrowth, transmural (TM) ingrowth or fallout (FO) from the blood. The limited TA and FO endothelialization, which occurs in humans, results in poor long-term patency in the small diameter position, where TM ingrowth may offer a clinically relevant alternative. To achieve sequential analysis of each mode of healing, loop grafts comprising anastomotically isolated angiopermissive polyurethane control grafts were abluminally sealed using either ePTFE wraps or solid polyurethane skins and implanted in the rat infrerenal aortic loop model for twelve weeks...
October 27, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29069222/effect-of-a-rosmarinic-acid-supplemented-hemodialysis-fluid-on-inflammation-of-human-vascular-endothelial-cells
#14
W-J Wang, M-H Cheng, J-H Lin, C-S Weng
Chronic systemic inflammation and repetitive damage of vascular endothelia by incompatible dialysis system are probable causes of cardiovascular disease in patients on dialysis. The present study aimed to assess in vitro biocompatibility and anti-inflammatory effect of hemodialysis fluid supplemented with rosmarinic acid (RA) using human umbilical vein endothelial cells (HUVEC). HUVECs (5×106 cells/mL) were pre-exposed to 1 μg/mL of lipopolysaccharides (LPS) and incubated with RA-supplemented hemodialysis fluid (HDF)...
October 19, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29067312/a-multicenter-randomized-placebo-controlled-trial-for-cilostazol-in-patients-with-mild-cognitive-impairment-the-comcid-study-protocol
#15
Satoshi Saito, Shinsuke Kojima, Naoya Oishi, Ryosuke Kakuta, Takakuni Maki, Fumihiko Yasuno, Kazuyuki Nagatsuka, Haruko Yamamoto, Hidenao Fukuyama, Masanori Fukushima, Masafumi Ihara
INTRODUCTION: There are currently no effective treatments preventing conversion from mild cognitive impairment (MCI) to Alzheimer's disease. Cilostazol is a selective type-3 phosphodiesterase inhibitor that ameliorates accumulation of amyloid-β and has prevented cognitive decline in rodent models. Furthermore, cilostazol is known to suppress platelet aggregation, protect vascular endothelia, dilate vessels, and increase cerebral blood flow. Beneficial effects have also been shown in observational cohort studies, demonstrating the need for a prospective clinical trial...
November 2016: Alzheimer's & Dementia: Translational Research & Clinical Interventions
https://www.readbyqxmd.com/read/29053877/trpv4-regulates-vascular-endothelial-permeability-during-colonic-inflammation-in-dextran-sulphate-sodium-induced-murine-colitis
#16
Kenjiro Matsumoto, Riho Yamaba, Ken Inoue, Daichi Utsumi, Takuya Tsukahara, Kikuko Amagase, Makoto Tominaga, Shinichi Kato
BACKGROUND AND PURPOSE: The transient receptor potential vanilloid 4 (TRPV4) is a nonselective cation channel involved in physical sensing in various tissue types. The present study aimed to elucidate the function and expression of TRPV4 in colonic vascular endothelial cells during dextran sulphate sodium (DSS)-induced colitis. EXPERIMENTAL APPROACH: The role of TRPV4 in the progression of colonic inflammation was examined in the 2 % DSS-induced murine colitis model using immunohistochemical analysis, western blotting, and Evans blue dye extrusion assay...
October 20, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29043853/microvascular-network-and-its-endothelial-cells-in-the-human-iris
#17
Hongfang Yang, Paula K Yu, Stephen J Cringle, Xinghuai Sun, Dao-Yi Yu
PURPOSE: The iris allows effective delivery of nutrients into the aqueous humor supplying the surrounded avascular tissues. However, possible underlying mechanisms of the iris vasculature have not been well established. This study aims to quantitatively assess the human iris vascular network, endothelial cell morphometries, and characterize endothelial junctions to better understand the properties of the iris vasculature. MATERIALS AND METHODS: The irises from human donor eyes were dissected and short fixed before float staining for VE-cadherin and claudin-5, f-actin and nuclei and flat-mounted for confocal imaging...
October 18, 2017: Current Eye Research
https://www.readbyqxmd.com/read/29042072/mir-200b-downregulates-kruppel-like-factor-2-klf2-during-acute-hypoxia-in-human-endothelial-cells
#18
Rafal Bartoszewski, Marcin Serocki, Anna Janaszak-Jasiecka, Sylwia Bartoszewska, Kinga Kochan-Jamrozy, Arkadiusz Piotrowski, Jarosław Króliczewski, James F Collawn
The role of microRNAs in controlling angiogenesis is recognized as a promising therapeutic target in both cancer and cardiovascular disorders. However, understanding a miRNA's pleiotropic effects on angiogenesis is a limiting factor for these types of therapeutic approaches. Using genome-wide next-generation sequencing, we examined the role of an antiangiogenic miRNA, miR-200b, in primary human endothelial cells. The results indicate that miR-200b has complex effects on hypoxia-induced angiogenesis in human endothelia and importantly, that many of the reported miR-200b effects using miRNA overexpression may not be representative of the physiological role of this miRNA...
December 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/29017464/respective-contribution-of-the-cephalic-neural-crest-and-mesoderm-to-six1-expressing-head-territories-in-the-avian-embryo
#19
Barbara F Fonseca, Gérard Couly, Elisabeth Dupin
BACKGROUND: Vertebrate head development depends on a series of interactions between many cell populations of distinct embryological origins. Cranial mesenchymal tissues have a dual embryonic source: - the neural crest (NC), which generates most of craniofacial skeleton, dermis, pericytes, fat cells, and tenocytes; and - the mesoderm, which yields muscles, blood vessel endothelia and some posterior cranial bones. The molecular players that orchestrate co-development of cephalic NC and mesodermal cells to properly construct the head of vertebrates remain poorly understood...
October 10, 2017: BMC Developmental Biology
https://www.readbyqxmd.com/read/28988496/6-gingerol-prevents-mehp-induced-dna-damage-in-human-umbilical-vein-endothelia-cells
#20
G Yang, X Gao, L Jiang, X Sun, X Liu, M Chen, X Yao, Q Sun, S Wang
Mono (2-ethylhexyl) phthalate (MEHP) is the principal metabolite of di (2-etylhexyl) phthalate, which is widely used as a plasticizer, especially in medical devices. MEHP has toxic effects on cardiovascular system. The aim of this study was to investigate the possibility that 6-gingerol may inhibit the oxidative DNA damage of MEHP in human umbilical vein endothelial cells (HUVECs) and the potential mechanism. The comet assay was used to monitor DNA strand breaks. We have shown that 6-gingerol significantly reduced the DNA strand breaks caused by MEHP...
November 2017: Human & Experimental Toxicology
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