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https://www.readbyqxmd.com/read/29031110/determination-of-l-glutamic-acid-and-%C3%AE-aminobutyric-acid-in-mouse-brain-tissue-utilizing-gc-ms-ms
#1
Christine A Farthing, Don E Farthing, Ronald E Gress, Douglas H Sweet
A rapid and selective method for the quantitation of neurotransmitters, l-Glutamic acid (GA) and γ-Aminobutyric acid (GABA), was developed and validated using gas chromatography-tandem mass spectrometry (GC-MS/MS). The novel method utilized a rapid online hot GC inlet gas phase sample derivatization and fast GC low thermal mass technology. The method calibration was linear from 0.5 to 100μg/mL, with limits of detections of 100ng/mL and 250ng/mL for GA and GABA, respectively. The method was used to investigate the effects of deletion of organic anion transporter 1 (Oat1) or Oat3 on murine CNS levels of GA and GABA at 3 and 18 mo of age, as compared to age matched wild-type (WT) animals...
October 9, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29028269/acceptance-and-adoption-of-biofortified-crops-in-low-and-middle-income-countries-a-systematic-review
#2
Elise F Talsma, Alida Melse-Boonstra, Inge D Brouwer
Biofortification of staple crops is a promising strategy for increasing the nutrient density of diets in order to improve human health. The willingness of consumers and producers to accept new crop varieties will determine whether biofortification can be successfully implemented. This review assessed sensory acceptance and adoption of biofortified crops and the determining factors for acceptance and adoption among consumers and producers in low- and middle-income countries. PubMed, Scopus, and Web of Science were searched for published reports...
October 1, 2017: Nutrition Reviews
https://www.readbyqxmd.com/read/29027330/poor-nutrition-from-first-foods-a-cross-sectional-study-of-complementary-feeding-of-infants-and-young-children-in-six-remote-aboriginal-communities-across-northern-australia
#3
Dympna Leonard, Danielle Aquino, Nyssa Hadgraft, Fintan Thompson, Julia V Marley
AIM: To describe the first foods of Aboriginal and Torres Strait Islander infants and young children who were recruited to a nutrition promotion and anaemia prevention program conducted from 2010 to 2012, in six remote communities across northern Australia. METHODS: Food records (24-hour diet history, food variety checklist) were completed on recruitment by interview with a parent or carer. Cross-sectional analysis assessed the proportion of participants consuming recommended and not-recommended foods and drinks and meeting recommendations for meal frequency and dietary diversity...
October 13, 2017: Nutrition & Dietetics: the Journal of the Dietitians Association of Australia
https://www.readbyqxmd.com/read/29025695/exploring-the-potential-of-a-wearable-camera-to-examine-the-early-obesogenic-home-environment-comparison-of-sensecam-images-to-the-home-environment-interview
#4
Stephanie Schrempft, Cornelia Hm van Jaarsveld, Abigail Fisher
BACKGROUND: The obesogenic home environment is usually examined via self-report, and objective measures are required. OBJECTIVE: This study explored whether the wearable camera SenseCam can be used to examine the early obesogenic home environment and whether it is useful for validation of self-report measures. METHODS: A total of 15 primary caregivers of young children (mean age of child 4 years) completed the Home Environment Interview (HEI)...
October 12, 2017: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/29024882/quantitative-mapping-of-elements-in-basil-leaves-ocimum-basilicum-based-on-cesium-concentration-and-growth-period-using-laser-ablation-icp-ms
#5
Jung Aa Ko, Naoki Furuta, Heung Bin Lim
Quantitative elemental mapping of metallic pollutants in sweet basil was studied by laser ablation (LA)-ICP-MS. For this, the sweet basil was cultivated in Hoagland nutrient solution spiked with 100 and 1000 ng mL(-1) of Cs for 10-60 days. Then, the Cs distribution in collected leaves was determined by LA-ICP-MS using lab-synthesized standard pellets based on NIST 1573a tomato leaves. For comparison, S, Ca, and K were also simultaneously determined in this measurement with a(13)C(+) signal from the leaves as an internal standard...
October 3, 2017: Chemosphere
https://www.readbyqxmd.com/read/29023543/a-mannitol-sorbitol-receptor-stimulates-dietary-intake-in-tribolium-castaneum
#6
Tomoyuki Takada, Ryoichi Sato, Shingo Kikuta
In insects, perception of chemical stimuli is involved in the acceptance or rejection of food. Gustatory receptors (Grs) that regulate external signals in chemosensory organs have been found in many insects. Tribolium castaneum, a major pest of stored products, possesses over 200 Gr genes. An expanded repertoire of Gr genes appears to be required for diet recognition in species that are generalist feeders; however, it remains unclear whether T. castaneum recognizes a suite of chemicals common to many products or whether its feeding is activated by specific chemicals, and whether its Grs are involved in feeding behavior...
2017: PloS One
https://www.readbyqxmd.com/read/29022681/body-image-dissatisfaction-and-weight-control-behaviour-in-slovak-adolescents
#7
Martina Bašková, Jana Holubčíková, Tibor Baška
OBJECTIVES: The aim of the study was to examine body-image dissatisfaction in adolescents aged 13-15 years reporting to be on a diet and to assess gender and age differences in weight-reduction behaviour in Slovak adolescents. METHODS: Data on a representative sample of 2,765 adolescents (mean age 14.37 years) from the Slovak part of Health Behaviour in School Aged Children Study carried out in 2014 were analysed. Self-reported body-image dissatisfaction and engagement in weight-reduction behaviour of 13 to 15-year-old adolescents was assessed using multiple logistic regression models...
September 2017: Central European Journal of Public Health
https://www.readbyqxmd.com/read/29022100/sea-buckthorn-decreases-and-delays-insulin-response-and-improves-glycaemic-profile-following-a-sucrose-containing-berry-meal-a-randomised-controlled-crossover-study-of-danish-sea-buckthorn-and-strawberries-in-overweight-and-obese-male-subjects
#8
Maria Wichmann Mortensen, Camilla Spagner, Cătălina Cuparencu, Arne Astrup, Anne Raben, Lars Ove Dragsted
PURPOSE: Berries and mixed berry products exert acute effects on postprandial glycaemia and insulinemia, but very few berries have been studied, and primarily in normal weight subjects. Sea buckthorn and strawberry are compositionally widely different berries and may likely produce different responses. The effects of strawberry and sea buckthorn on postprandial glycaemia and insulinemia were examined in overweight or obese male subjects. Subjective appetite sensations and ad libitum intake were also examined...
October 11, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/29021807/c4-protein-of-sweet-potato-leaf-curl-virus-regulates-brassinosteroid-signaling-pathway-through-interaction-with-atbin2-and-affects-male-fertility-in-arabidopsis
#9
Huiping Bi, Weijuan Fan, Peng Zhang
Sweepoviruses have been identified globally and cause substantial yield losses and cultivar decline in sweet potato. This study aimed to investigate the interaction between sweepovirus and plant host by analyzing the function of the viral protein C4 of Sweet potato leaf curl virus-Jiangsu (SPLCV-JS), a sweepovirus cloned from diseased sweet potato plants in East China. Ectopic expression of the C4 in Arabidopsis altered plant development drastically with phenotypic changes including leaf curling, seedling twisting, deformation of floral tissues and reduction of pollen fertility, and seed number...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29020414/separate-and-combined-associations-of-obesity-and-metabolic-health-with-coronary-heart-disease-a-pan-european-case-cohort-analysis
#10
Camille Lassale, Ioanna Tzoulaki, Karel G M Moons, Michael Sweeting, Jolanda Boer, Laura Johnson, José María Huerta, Claudia Agnoli, Heinz Freisling, Elisabete Weiderpass, Patrik Wennberg, Daphne L van der A, Larraitz Arriola, Vassiliki Benetou, Heiner Boeing, Fabrice Bonnet, Sandra M Colorado-Yohar, Gunnar Engström, Anne K Eriksen, Pietro Ferrari, Sara Grioni, Matthias Johansson, Rudolf Kaaks, Michail Katsoulis, Verena Katzke, Timothy J Key, Giuseppe Matullo, Olle Melander, Elena Molina-Portillo, Concepción Moreno-Iribas, Margareta Norberg, Kim Overvad, Salvatore Panico, J Ramón Quirós, Calogero Saieva, Guri Skeie, Annika Steffen, Magdalena Stepien, Anne Tjønneland, Antonia Trichopoulou, Rosario Tumino, Yvonne T van der Schouw, W M Monique Verschuren, Claudia Langenberg, Emanuele Di Angelantonio, Elio Riboli, Nicholas J Wareham, John Danesh, Adam S Butterworth
Aims: The hypothesis of 'metabolically healthy obesity' implies that, in the absence of metabolic dysfunction, individuals with excess adiposity are not at greater cardiovascular risk. We tested this hypothesis in a large pan-European prospective study. Methods and results: We conducted a case-cohort analysis in the 520 000-person European Prospective Investigation into Cancer and Nutrition study ('EPIC-CVD'). During a median follow-up of 12.2 years, we recorded 7637 incident coronary heart disease (CHD) cases...
August 14, 2017: European Heart Journal
https://www.readbyqxmd.com/read/29020092/construction-of-a-genetic-map-using-est-ssr-markers-and-qtl-analysis-of-major-agronomic-characters-in-hexaploid-sweet-potato-ipomoea-batatas-l-lam
#11
Jin-Hee Kim, Il Kyung Chung, Kyung-Min Kim
The Sweet potato, Ipomoea batatas (L.) Lam, is difficult to study in genetics and genomics because it is a hexaploid. The sweet potato study not have been performed domestically or internationally. In this study was performed to construct genetic map and quantitative trait loci (QTL) analysis. A total of 245 EST-SSR markers were developed, and the map was constructed by using 210 of those markers. The total map length was 1508.1 cM, and the mean distance between markers was 7.2 cM. Fifteen characteristics were investigated for QTLs analysis...
2017: PloS One
https://www.readbyqxmd.com/read/29019082/loss-of-the-nutrient-sensor-tas1r3-leads-to-reduced-bone-resorption
#12
Michael S Eaton, Nicholas Weinstein, Jordan B Newby, Maggie M Plattes, Hanna E Foster, Jon W Arthur, Taylor D Ward, Stephen R Shively, Ryann Shor, Justin Nathan, Hannah M Davis, Lilian I Plotkin, Eric M Wauson, Brian J Dewar, Aaron Broege, Jonathan W Lowery
The taste receptor type 1 (TAS1R) family of heterotrimeric G protein-coupled receptors participates in monitoring energy and nutrient status. TAS1R member 3 (TAS1R3) is a bi-functional protein that recognizes amino acids such as L-glycine and L-glutamate or sweet molecules such as sucrose and fructose when dimerized with TAS1R member 1 (TAS1R1) or TAS1R member 2 (TAS1R2), respectively. It was recently reported that deletion of TAS1R3 expression in Tas1R3 mutant mice leads to increased cortical bone mass but the underlying cellular mechanism leading to this phenotype remains unclear...
October 10, 2017: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/29018778/cancer-preventive-properties-of-an-anthocyanin-enriched-sweet-potato-in-the-apc-min-mouse-model
#13
Khalid Asadi, Lynnette R Ferguson, Martin Philpott, Nishi Karunasinghe
BACKGROUND: Anthocyanin-rich foods and preparations have been reported to reduce the risk of life-style related diseases, including cancer. The SL222 sweet potato, a purple-fleshed cultivar developed in New Zealand, accumulates high levels of anthocyanins in its storage root. METHODS: We examined the chemopreventative properties of the SL222 sweet potato in the C57BL/6J-APC (MIN/+) (APC(MIN)) mouse, a genetic model of colorectal cancer. APC(MIN) and C57BL/6J wild-type mice (n=160) were divided into four feeding groups consuming diets containing 10% SL222 sweet potato flesh, 10% SL222 sweet potato skin, or 0...
September 2017: Journal of Cancer Prevention
https://www.readbyqxmd.com/read/29018460/the-first-genetic-map-in-sweet-osmanthus-osmanthus-fragrans-lour-using-specific-locus-amplified-fragment-sequencing
#14
Yanxia He, Wangjun Yuan, Meifang Dong, Yuanji Han, Fude Shang
Osmanthus fragrans is an ornamental plant of substantial commercial value, and no genetic linkage maps of this species have previously been reported. Specific-locus amplified fragment sequencing (SLAF-seq) is a recently developed technology that allows massive single nucleotide polymorphisms (SNPs) to be identified and high-resolution genotyping. In our current research, we generated the first genetic map of O. fragrans using SLAF-seq, which is composed with 206.92 M paired-end reads and 173,537 SLAF markers...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29018310/virus-incidence-of-sweet-potato-in-korea-from-2011-to-2014
#15
Jaedeok Kim, Jung Wook Yang, Hae-Ryun Kwak, Mi-Kyeong Kim, Jang-Kyun Seo, Mi-Nam Chung, Hyeong-Un Lee, Kyeong-Bo Lee, Sang Sik Nam, Chang-Seok Kim, Gwan-Seok Lee, Jeong-Soo Kim, Sukchan Lee, Hong-Soo Choi
A nationwide survey was performed to investigate the current incidence of viral diseases in Korean sweet potatoes for germplasm and growing fields from 2011 to 2014. A total of 83.8% of the germplasm in Korea was infected with viruses in 2011. Commercial cultivars that were used to supply growing fields were infected at a rate of 62.1% in 2012. Among surveyed viruses, the incidence of five Potyvirus species that infect sweet potato decreased between 2012 and 2013, and then increased again in 2014. Representatively, the incidence of Sweet potato feathery mottle virus (SPFMV) was 87...
October 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/29016251/addressing-the-problem-of-obesity-and-associated-cardiometabolic-risk-in-black-south-african-women-time-for-action
#16
Julia H Goedecke
The PhD thesis of Gradidge, entitled 'Factors associated with obesity and metabolic syndrome in an ageing cohort of black women living in Soweto, Johannesburg (Study of Women in and Entering Endocrine Transition [SWEET])', attempts to understand the determinants of obesity and metabolic syndrome (MetS) in a population of urban-dwelling black South African women. A conceptual framework is presented, which positions obesity as the central risk factor for MetS, and includes the possible influence of socioeconomic status, lifestyle behaviours and body size perceptions, as key determinants of obesity...
2017: Global Health Action
https://www.readbyqxmd.com/read/28994341/-a-sweet-smile-the-modulatory-role-of-emotion-in-how-extrinsic-factors-influence-taste-evaluation
#17
Qian Janice Wang, Charles Spence
It has recently been demonstrated that the reported tastes/flavours of food/beverages can be modulated by means of external visual and auditory stimuli such as typeface, shapes, and music. The present study was designed to assess the role of the emotional valence of the product-extrinsic stimuli in such crossmodal modulations of taste. Participants evaluated samples of mixed fruit juice whilst simultaneously being presented with auditory or visual stimuli having either positive or negative valence. The soundtracks had either been harmonised with consonant (positive valence) or dissonant (negative valence) musical intervals...
October 10, 2017: Cognition & Emotion
https://www.readbyqxmd.com/read/28994214/effect-of-replacing-alfalfa-hay-with-a-mixture-of-cassava-foliage-silage-and-sweet-potato-vine-silage-on-ruminal-and-intestinal-digestion-in-sheep
#18
Hoang Lam Dang, Renlong Lv, Taketo Obitsu, Toshihisa Sugino
This study aimed to investigate the effects of replacing alfalfa hay (AH) with a mixture of cassava foliage silage and sweet potato vine silage (CSP) (1:1 on a dry matter (DM) basis) on ruminal and intestinal nutrient digestion in sheep. Four wethers were fed a control diet containing 35% of AH and two treatment diets containing 15% and 30% of the CSP as substitute for AH at 1.5 times the metabolizable energy required for maintenance. Replacing AH with the CSP silage did not affect DM intake, whereas it linearly increased (P < 0...
October 9, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28993555/complete-chloroplast-genome-and-45s-nrdna-sequences-of-the-medicinal-plant-species-glycyrrhiza-glabra-and-glycyrrhiza-uralensis
#19
Sang-Ho Kang, Jeong-Hoon Lee, Hyun Oh Lee, Byoung Ohg Ahn, So Youn Won, Seong-Han Sohn, Jung Sun Kim
Glycyrrhiza uralensis and G. glabra, members of the Fabaceae, are medicinally important species that are native to Asia and Europe. Extracts from these plants are widely used as natural sweeteners because of their much greater sweetness than sucrose. In this study, the three complete chloroplast genomes and five 45S nuclear ribosomal (nr)DNA sequences of these two licorice species and an interspecific hybrid are presented. The chloroplast genomes of G. glabra, G. uralensis and G. glabra × G. uralensis were 127,895 bp, 127,716 bp and 127,939 bp, respectively...
October 6, 2017: Genes & Genetic Systems
https://www.readbyqxmd.com/read/28992329/transcription-factor-citerf71-activates-the-terpene-synthase-gene-cittps16-involved-in-the-synthesis-of-e-geraniol-in-sweet-orange-fruit
#20
Xiang Li, Yaying Xu, Shuling Shen, Xueren Yin, Harry Klee, Bo Zhang, Kunsong Chen
The unique flavor of Citrus fruit depends on complex combinations of soluble sugars, organic acids, and volatile compounds. The monoterpene E-geraniol is an important volatile, contributing to flavor in sweet orange (Citrus sinensis Osbeck). Moreover, antifungal activity of E-geraniol has also been observed. However, the terpene synthase (TPS) responsible for its synthesis has not been identified in sweet orange. Terpene synthase 16 (CitTPS16) was shown to catalyze synthesis of E-geraniol in vitro, and transient overexpression of CitTPS16 in fruits and leaves of Newhall sweet orange resulted in E-geraniol accumulation in vivo...
September 7, 2017: Journal of Experimental Botany
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