Read by QxMD icon Read

Urine PCA 3

Bo Sun, Xubin Wang, Ruili Cao, Qi Zhang, Qiao Liu, Meifeng Xu, Ming Zhang, Xiangbo Du, Fangting Dong, Xianzhong Yan
ETHNOPHARMACOLOGICAL RELEVANCE: Fuzi, the processed lateral root of Aconitum carmichaelii Debeaux, is a traditional Chinese medicine used for its analgesic, antipyretic, anti-rheumatoid arthritis and anti-inflammation effects; however, it is also well known for its toxicity. Gancao, the root of Glycyrrhiza uralensis Fisch., is often used concurrently with Fuzi to alleviate its toxicity. However, the mechanism of detoxication is still not well clear. AIM OF THE STUDY: In this study, the effect of Gancao on the metabolic changes induced by Fuzi was investigated by NMR-based metabonomic approaches...
October 13, 2016: Journal of Ethnopharmacology
Amalia Katafigioti, Ioannis Katafigiotis, Stavros Sfoungaristos, Christos Alamanis, Konstantinos Stravodimos, Ioannis Anastasiou, Eleni Roumelioti, Mordechai Duvdevani, Constantinos Constantinides
OBJECTIVE: To search which category of proteins can be detected in urine in order to examine subsequently its ability to improve our accuracy for the diagnosis of Prostate Cancer (PCa) as biomarkers in clinical useful fluids like urine and serum. Material and method(s): Urine samples of 127 patients were obtained after a vigorous transrectal prostatic massage to both lobes. The patients were considered to have a high risk for PCa according to their PSA (> 4 ng/ml), their digital rectal examination (DRE) (positive for suspicious prostatic lesions) or to their abnormal PSA kinetics (PSA velocity (PSAV > 0...
October 5, 2016: Archivio Italiano di Urologia, Andrologia
Matthew J Roberts, Renee S Richards, Clement W K Chow, Suhail A R Doi, Horst Joachim Schirra, Marion Buck, Hemamali Samaratunga, Joanna Perry-Keene, Diane Payton, John Yaxley, Martin F Lavin, Robert A Gardiner
BACKGROUND: Prostate cancer (PCa) diagnosis requires improvement with the aid of more accurate biomarkers. Postejaculate urethral washings (PEUW) could be a physiological equivalent to urine obtained following rectal prostatic massage, the current basis for the prostate cancer antigen 3 (PCA3) test. The aim of this study was to investigate if PEUW contained prostate-based material, evidenced by the presence of prostate specific antigen (PSA), and to evaluate the diagnostic performance of PEUW-based biomarkers...
September 2016: Prostate International
Taoguang Huo, Ying Fang, Longshan Zhao, Zhili Xiong, Yinghua Zhang, Yanlei Wang, Cong Feng, Mingmei Yuan, Shouyun Wang, Mo Chen, Hong Jiang
ETHNOPHARMACOLOGICAL RELEVANCE: Realgar has been used as a traditional Chinese medicine (TCM) for thousands of years. Recently, a number of realgar or realgar-containing medicines poisoning cases have been reported. However, the toxicological mechanism of realgar has not been clearly clarified. In present study, the subchronic hepatotoxicity of realgar on mice was investigated using (1)HNMR-based metabonomic approaches. MATERIALS AND METHODS: Twenty-eight male mice were divided into control group and low (0...
July 1, 2016: Journal of Ethnopharmacology
Yue Huang, Yunhai Bo, Xiao Wu, Qiuyi Wang, Feng Qin, Longshan Zhao, Zhili Xiong
Gushudan, a Chinese compound formulation based on the theory of traditional Chinese medicine and desgined to treat osteoporosis. However, its intergated intervention effective mechanism in vivo is not well understood. In this study, an intergated serum and urinary metabonomic strategy based on UPLC-MS technique have been developed to increase the understanding of the metabolism characters of osteoporosis and to investigate the holistic therapeutic efficacy of Gushudan on prednisolone-induced osteoporosis rat model...
August 1, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Mihaela Stoia, Zelimir Kurtanjek, Simona Oancea
OBJECTIVE: This study aimed to provide the toxicological profile of some lead-exposed workers and obtain a predictive model for lead poisoning. METHODS: Data regarding external and absorbed exposure were collected from 585 subjects employed in ten metallurgical production departments. Airborne lead concentration, blood lead level (BLL), cumulative blood lead index (CBLI), urine delta-aminolevulinic acid (DALA), age, workplace/section, exposure period, and whether reported lead poisoning as occupational disease were examined using ANOVA, and, post-ANOVA, Pearson correlation matrix, PCA (principal component analysis), decision-tree modeling, and logistic modeling...
July 2016: American Journal of Industrial Medicine
Clint M Alfaro, Alan K Jarmusch, Valentina Pirro, Kevin S Kerian, Timothy A Masterson, Liang Cheng, R Graham Cooks
Touch spray-mass spectrometry (TS-MS) is an ambient ionization technique (ionization of unprocessed samples in the open air) that may find intraoperative applications in quickly identifying the disease state of cancerous tissues and in defining surgical margins. In this study, TS-MS was performed on fresh kidney tissue (∼1-5 cm(3)), within 1 h of resection, from 21 human subjects afflicted by renal cell carcinoma (RCC). The preliminary diagnostic value of TS-MS data taken from freshly resected tissue was evaluated...
August 2016: Analytical and Bioanalytical Chemistry
Eugenio Alladio, Roberto Caruso, Enrico Gerace, Eleonora Amante, Alberto Salomone, Marco Vincenti
The Technical Document TD2014EAAS was drafted by the World Anti-Doping Agency (WADA) in order to fight the spread of endogenous anabolic androgenic steroids (EAAS) misuse in several sport disciplines. In particular, adoption of the so-called Athlete Biological Passport (ABP) - Steroidal Module allowed control laboratories to identify anomalous EAAS concentrations within the athletes' physiological urinary steroidal profile. Gas chromatography (GC) combined with mass spectrometry (MS), indicated by WADA as an appropriate technique to detect urinary EAAS, was utilized in the present study to develop and fully-validate an analytical method for the determination of all EAAS markers specified in TD2014EAAS, plus two further markers hypothetically useful to reveal microbial degradation of the sample...
May 30, 2016: Analytica Chimica Acta
Allison H Feibus, Oliver Sartor, Krishnarao Moparty, Kevin Chagin, Michael W Kattan, Elisa Ledet, Justin Levy, Benjamin Lee, Raju Thomas, Jonathan L Silberstein
PURPOSE: Prostate specific antigen has decreased performance characteristics for the detection of prostate cancer in African-American men. We evaluated urinary PCA3 and TMPRSS2:ERG in a racially diverse group of men. MATERIALS AND METHODS: After institutional review board approval, post-examination urine was prospectively collected before prostate biopsy. PCA3 and TMPRSS2:ERG RNA copies were quantified using transcription mediated amplification assays (Hologic, San Diego, California)...
October 2016: Journal of Urology
James McKiernan, Michael J Donovan, Vince O'Neill, Stefan Bentink, Mikkel Noerholm, Susan Belzer, Johan Skog, Michael W Kattan, Alan Partin, Gerald Andriole, Gordon Brown, John T Wei, Ian M Thompson, Peter Carroll
IMPORTANCE: Overdiagnosis and overtreatment of indolent prostate cancer (PCA) is a serious health issue in most developed countries. There is an unmet clinical need for noninvasive, easy to administer, diagnostic assays to help assess whether a prostate biopsy is warranted. OBJECTIVE: To determine the performance of a novel urine exosome gene expression assay (the ExoDx Prostate IntelliScore urine exosome assay) plus standard of care (SOC) (ie, prostate-specific antigen [PSA] level, age, race, and family history) vs SOC alone for discriminating between Gleason score (GS)7 and GS6 and benign disease on initial biopsy...
July 1, 2016: JAMA Oncology
Run Guo, Qunxing Tang, Yi Ye, Xiang Lu, Fan Chen, Xinhua Dai, Youyi Yan, Linchuan Liao
OBJECTIVE: The aim of this study was to examine whether or not there was a gender difference in CPP (conditioned placed preference) induced by ketamine and to further explore the effect of sex on metabolic responses to ketamine inducing in SD rats. METHODS: We measured ketamine-induced conditioned place preference and ketamine-induced metabolic changes in urine by using (1)H nuclear magnetic resonance (NMR) coupled with principal component analysis (PCA), partial least squares (PLS) and orthogonal signal correction (OSC) analysis...
June 2016: Regulatory Toxicology and Pharmacology: RTP
Prosper Kanyong, Sean Rawlinson, James Davis
Prostate cancer (PCa) is a significant cause of morbidity and mortality and the most common cancer in men in Europe, North America, and some parts of Africa. The established methods for detecting PCa are normally based on tests using Prostate Specific Antigen (PSA) in blood, Prostate cancer antigen 3 (PCA3) in urine and tissue Alpha-methylacyl-CoA racemase (AMACR) as tumour markers in patient samples. Prior to the introduction of PSA in clinics, prostatic acid phosphatase (PAP) was the most widely used biomarker...
2016: Journal of Cancer
Ali Muhsen Ali, Mia Burleigh, Evangelia Daskalaki, Tong Zhang, Chris Easton, David G Watson
Ten physically active subjects underwent two cycling exercise trials. In the first, aerobic capacity (VO2max) was determined and the second was a 45 min submaximal exercise test. Urine samples were collected separately the day before (day 1) , the day of (day 2), and the day after (day 3) the submaximal exercise test (12 samples per subject). Metabolomic profiling of the samples was carried out using hydrophilic interaction chromatography (HILIC) coupled to an Orbitrap Exactive mass spectrometer. Data were extracted, database searched and then subjected to principle components (PCA) and orthogonal partial least squares (OPLSDA) modelling...
2016: Metabolites
Chih-Yung Chiu, Kuo-Wei Yeh, Gigin Lin, Meng-Han Chiang, Shu-Chen Yang, Wei-Ju Chao, Tsung-Chieh Yao, Ming-Han Tsai, Man-Chin Hua, Sui-Ling Liao, Shen-Hao Lai, Mei-Ling Cheng, Jing-Long Huang
OBJECTIVES: A detailed understanding of the metabolic processes governing rapid growth in early life is still lacking. The aim of this study was to investigate the age-related metabolic changes in healthy children throughout early childhood. METHODS: Healthy children from a birth cohort were enrolled in this study from birth through 4 years of age. Urinary metabolites were assessed at 6 months, and 1, 2, 3, and 4 yr of age by using 1H-nuclear magnetic resonance (NMR) spectroscopy coupled with multivariate statistical analysis including principal components analysis (PCA) and partial least-squares discriminant analysis (PLS-DA)...
2016: PloS One
Bruce C Wainman, James S Kesner, Ian D Martin, Juliana W Meadows, Edward F Krieg, Evert Nieboer, Leonard J Tsuji
Persistent organohalogens (POHs) and metals have been linked to alterations in menstrual cycle function and fertility in humans. The Cree First Nations people living near James Bay in Ontario and Quebec, Canada, have elevated levels of POHs, mercury and lead compared to other Canadians. The present study examines the interrelationships between selected POHs and elements on menstrual cycle function in these Cree women. Menstrual cycle characteristics were derived from structured daily diaries and endocrine measurements from daily urine samples collected during one cycle for 42 women age 19-42...
April 2016: Chemosphere
Colleen N Biggs, Khurram M Siddiqui, Ali A Al-Zahrani, Siddika Pardhan, Sabine I Brett, Qiu Q Guo, Jun Yang, Philipp Wolf, Nicholas E Power, Paul N Durfee, Connor D MacMillan, Jason L Townson, Jeffrey C Brinker, Neil E Fleshner, Jonathan I Izawa, Ann F Chambers, Joseph L Chin, Hon S Leong
BACKGROUND: Extracellular vesicles released by prostate cancer present in seminal fluid, urine, and blood may represent a non-invasive means to identify and prioritize patients with intermediate risk and high risk of prostate cancer. We hypothesize that enumeration of circulating prostate microparticles (PMPs), a type of extracellular vesicle (EV), can identify patients with Gleason Score≥4+4 prostate cancer (PCa) in a manner independent of PSA. PATIENTS AND METHODS: Plasmas from healthy volunteers, benign prostatic hyperplasia patients, and PCa patients with various Gleason score patterns were analyzed for PMPs...
February 23, 2016: Oncotarget
Francesca Sanguedolce, Antonella Cormio, Matteo Brunelli, Alessandro D'Amuri, Giuseppe Carrieri, Pantaleo Bufo, Luigi Cormio
Prostate cancer (PCa) is one of the most common male malignancies. Serum prostate-specific antigen (PSA) is one of the most valuable biomarkers in tumor biology and remains the standard marker in detecting and monitoring PCa. However, the high number of serum PSA false positive and false negative results make the identification of novel biomarkers extremely welcome to improve our diagnostic accuracy in detecting PCa and distinguishing the aggressive from the indolent ones. In this study, we analyzed the current role of urinary gene fusion transcripts involving v-ets erythroblastosis virus E26 oncogene homolog, commonly known as ERG, and the androgen-regulated gene transmembrane protease, serine 2 (TMPRSS2), as a biomarker for PCa...
April 2016: Clinical Genitourinary Cancer
George Ho Man Chan, Emmie Ngai Man Ho, David Kwan Kon Leung, Kin Sing Wong, Terence See Ming Wan
The use of anabolic androgenic steroids (AAS) is prohibited in both human and equine sports. The conventional approach in doping control testing for AAS (as well as other prohibited substances) is accomplished by the direct detection of target AAS or their characteristic metabolites in biological samples using hyphenated techniques such as gas chromatography or liquid chromatography coupled with mass spectrometry. Such an approach, however, falls short when dealing with unknown designer steroids where reference materials and their pharmacokinetics are not available...
January 5, 2016: Analytical Chemistry
Wei Wei, Jiangyong Leng, Hongxiang Shao, Weidong Wang
BACKGROUND: To improve the prediction of prostate cancer (PCA) risk and pathological type of PCA by non-invasive approaches before performing prostatic biopsy are the current challenges for the management of PCA. The aim of this present study was evaluate the clinical validity of prostate cancer associated 3 (PCA3) gene in the prediction of PCA and the correlations between the PCA3 level and prognostic factors. METHODS: A total of 207 patients with suspected prostate cancer in Ningbo No...
2015: International Journal of Clinical and Experimental Medicine
Bertrand Neveu, Pallavi Jain, Bernard Têtu, Lily Wu, Yves Fradet, Frédéric Pouliot
Targeting specifically primary prostate cancer (PCa) cells for immune therapy, gene therapy or molecular imaging is of high importance. The PCA3 long non-coding RNA is a unique PCa biomarker and oncogene that has been widely studied. This gene has been mainly exploited as an accurate diagnostic urine biomarker for PCa detection. In this study, the PCA3 promoter was introduced into a new transcriptional amplification system named the 3-Step Transcriptional Amplification System (PCA3-3STA) and cloned into type 5 adenovirus...
January 12, 2016: Oncotarget
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"