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Urine PCA 3

A I Neimark, M A Tachalov, B A Neimark, D V Torbik, D D Arzamastsev
AIM: To evaluate the efficacy and safety of superselective embolization and chemoembolization of the prostatic artery as an independent method of treating prostate cancer and BPH. MATERIALS AND METHODS: From 2004 to 2015, 116 patients with BPH (mean age 73.2+/-5.2 years) underwent prostatic artery embolization. Mean prostate volume ranged from 55 to 296 cc3. From 2011 to 2015, 37 patients with prostate cancer (mean age 72.3+/-2.06 years) were treated with X-ray-guided endovascular chemoembolization...
April 2017: Urologii︠a︡
Karl H Pang, Derek J Rosario, Susan L Morgan, James W F Catto
BACKGROUND: Prostate Cancer 3 (PCA3) is a long non-coding RNA (ncRNA) upregulated in prostate cancer (PCa). We recently identified a short ncRNA expressed from intron 1 of PCA3. Here we test the ability of this ncRNA to predict the presence of cancer in men with a biopsy without PCa. METHODS: We selected men whose initial biopsy did not identify PCa and selected matched cohorts whose subsequent biopsies revealed PCa or benign tissue. We extracted RNA from the initial biopsy and measured PCA3-shRNA2, PCA3 and PSA (qRT-PCR)...
2017: PloS One
Fan Feng, Jianwu Zheng, Peng Qin, Tao Han, Dayun Zhao
For the rapid and robust detection of both parent clenbuterol (CLB) and its metabolites in swine urine samples, a novel quartz crystal microbalance (QCM) sensor array for CLB detection based on molecularly imprinted polymers (MIPs) was developed in this investigation. At first, clenbuterol and the structural analogs of its metabolites, 4-Aminohippuric acid (AHA) and 4-hydroxymandelic acid (HMA), were chosen as molecular templates. Through computational molecular modeling, the optimum ratio between the functional monomer and molecular template was selected...
May 15, 2017: Talanta
Hasan Dani, Stacy Loeb
PURPOSE OF REVIEW: This article intends to review biomarkers derived from blood, urine, and tissue that can aid in the diagnosis of prostate cancer (PCa). RECENT FINDINGS: PCa screening requires tools that complement prostate-specific antigen (PSA) with a higher specificity for clinically significant disease. Novel blood biomarkers, such as the Prostate Health Index (phi) and 4Kscore, utilize isoforms of PSA to more accurately predict high-grade PCa than traditional tools such as PSA and the percentage free-to-total PSA...
May 2017: Current Opinion in Urology
Mo Zhang, Lizhu Chen, Zhengwei Yuan, Zeyu Yang, Yue Li, Liping Shan, Bo Yin, Xiang Fei, Jianing Miao, Yongsheng Song
Prostate cancer (PCa) is one of the most common malignant tumors and a major cause of cancer-related death for men worldwide. The aim of our study was to identify potential non-invasive serum and expressed prostatic secretion (EPS)-urine biomarkers for accurate diagnosis of PCa. Here, we performed a combined isobaric tags for relative and absolute quantification (iTRAQ) proteomic analysis to compare protein profiles using pooled serum and EPS-urine samples from 4 groups of patients: benign prostate hyperplasia (BPH), high grade prostatic intraepithelial neoplasia (HGPIN), localized PCa and metastatic PCa...
November 2016: Discovery Medicine
Raghunath Pariyani, Intan Safinar Ismail, Amalina Azam, Alfi Khatib, Faridah Abas, Khozirah Shaari, Hazilawati Hamza
Orthosiphon stamineus (OS) is a popular medicinal herb used in traditional Chinese medicine as a diuretic agent and for renal system disorders. This study employed (1)H NMR based metabolomics approach to investigate the possible protective activity of OS in cisplatin induced nephrotoxicity owing to its diuretic and antioxidant activities. Aqueous (OSAE) and 50% aqueous ethanolic (OSFE) extracts of OS leaves were orally administered at 400mg/kg BW doses to rats which were then intraperitoneally injected with cisplatin at 5mg/kg BW dose...
December 8, 2016: Journal of Pharmaceutical and Biomedical Analysis
Henna Kekki, Mari Peltola, Sandra van Vliet, Chris Bangma, Yvette van Kooyk, Kim Pettersson
OBJECTIVES: The objective was to study the differences in PSA fucosylation obtained from LNCaP and PC-3 prostate cancer cell lines, seminal plasma PSA and recombinant precursor form of PSA expressed in baculovirus, using Aleuria aurantia lectin (AAL). The aim was to assess whether differences in fucosylation (Fucα1-6/3GlcNAc carbohydrates) of PSA either in urine, blood or tissue enable the discrimination of patients with prostate cancer (PCa) from benign prostatic hyperplasia (BPH) and young males...
January 2017: Clinical Biochemistry
Antonia M Calafat, Xiaoyun Ye, Liza Valentin-Blasini, Zheng Li, Mary E Mortensen, Lee-Yang Wong
BACKGROUND: General population human biomonitoring programs such as the National Health and Nutrition Examination Survey (NHANES) in the United States suggest that chemical exposures are common. Exposures during childhood may affect health later in life, but biomonitoring data in NHANES among pre-school aged children are limited. METHODS: A convenience group of 122 3-5year old American boys and girls were recruited in 2013 for a pilot study to assess the feasibility of collecting urine from young children and analyzing it for select chemical exposure biomarkers for future NHANES...
March 2017: International Journal of Hygiene and Environmental Health
Bo Sun, Xubin Wang, Ruili Cao, Qi Zhang, Qiao Liu, Meifeng Xu, Ming Zhang, Xiangbo Du, Fangting Dong, Xianzhong Yan
ETHNOPHARMACOLOGICAL RELEVANCE: Fuzi, the processed lateral root of Aconitum carmichaelii Debeaux, is a traditional Chinese medicine used for its analgesic, antipyretic, anti-rheumatoid arthritis and anti-inflammation effects; however, it is also well known for its toxicity. Gancao, the root of Glycyrrhiza uralensis Fisch., is often used concurrently with Fuzi to alleviate its toxicity. However, the mechanism of detoxication is still not well clear. AIM OF THE STUDY: In this study, the effect of Gancao on the metabolic changes induced by Fuzi was investigated by NMR-based metabonomic approaches...
December 4, 2016: Journal of Ethnopharmacology
Amalia Katafigioti, Ioannis Katafigiotis, Stavros Sfoungaristos, Christos Alamanis, Konstantinos Stravodimos, Ioannis Anastasiou, Eleni Roumelioti, Mordechai Duvdevani, Constantinos Constantinides
OBJECTIVE: To search which category of proteins can be detected in urine in order to examine subsequently its ability to improve our accuracy for the diagnosis of Prostate Cancer (PCa) as biomarkers in clinical useful fluids like urine and serum. Material and method(s): Urine samples of 127 patients were obtained after a vigorous transrectal prostatic massage to both lobes. The patients were considered to have a high risk for PCa according to their PSA (> 4 ng/ml), their digital rectal examination (DRE) (positive for suspicious prostatic lesions) or to their abnormal PSA kinetics (PSA velocity (PSAV > 0...
October 5, 2016: Archivio Italiano di Urologia, Andrologia
Matthew J Roberts, Renee S Richards, Clement W K Chow, Suhail A R Doi, Horst Joachim Schirra, Marion Buck, Hemamali Samaratunga, Joanna Perry-Keene, Diane Payton, John Yaxley, Martin F Lavin, Robert A Gardiner
BACKGROUND: Prostate cancer (PCa) diagnosis requires improvement with the aid of more accurate biomarkers. Postejaculate urethral washings (PEUW) could be a physiological equivalent to urine obtained following rectal prostatic massage, the current basis for the prostate cancer antigen 3 (PCA3) test. The aim of this study was to investigate if PEUW contained prostate-based material, evidenced by the presence of prostate specific antigen (PSA), and to evaluate the diagnostic performance of PEUW-based biomarkers...
September 2016: Prostate International
Taoguang Huo, Ying Fang, Longshan Zhao, Zhili Xiong, Yinghua Zhang, Yanlei Wang, Cong Feng, Mingmei Yuan, Shouyun Wang, Mo Chen, Hong Jiang
ETHNOPHARMACOLOGICAL RELEVANCE: Realgar has been used as a traditional Chinese medicine (TCM) for thousands of years. Recently, a number of realgar or realgar-containing medicines poisoning cases have been reported. However, the toxicological mechanism of realgar has not been clearly clarified. In present study, the subchronic hepatotoxicity of realgar on mice was investigated using (1)HNMR-based metabonomic approaches. MATERIALS AND METHODS: Twenty-eight male mice were divided into control group and low (0...
November 4, 2016: Journal of Ethnopharmacology
Yue Huang, Yunhai Bo, Xiao Wu, Qiuyi Wang, Feng Qin, Longshan Zhao, Zhili Xiong
Gushudan, a Chinese compound formulation based on the theory of traditional Chinese medicine and desgined to treat osteoporosis. However, its intergated intervention effective mechanism in vivo is not well understood. In this study, an intergated serum and urinary metabonomic strategy based on UPLC-MS technique have been developed to increase the understanding of the metabolism characters of osteoporosis and to investigate the holistic therapeutic efficacy of Gushudan on prednisolone-induced osteoporosis rat model...
August 1, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Mihaela Stoia, Zelimir Kurtanjek, Simona Oancea
OBJECTIVE: This study aimed to provide the toxicological profile of some lead-exposed workers and obtain a predictive model for lead poisoning. METHODS: Data regarding external and absorbed exposure were collected from 585 subjects employed in ten metallurgical production departments. Airborne lead concentration, blood lead level (BLL), cumulative blood lead index (CBLI), urine delta-aminolevulinic acid (DALA), age, workplace/section, exposure period, and whether reported lead poisoning as occupational disease were examined using ANOVA, and, post-ANOVA, Pearson correlation matrix, PCA (principal component analysis), decision-tree modeling, and logistic modeling...
July 2016: American Journal of Industrial Medicine
Clint M Alfaro, Alan K Jarmusch, Valentina Pirro, Kevin S Kerian, Timothy A Masterson, Liang Cheng, R Graham Cooks
Touch spray-mass spectrometry (TS-MS) is an ambient ionization technique (ionization of unprocessed samples in the open air) that may find intraoperative applications in quickly identifying the disease state of cancerous tissues and in defining surgical margins. In this study, TS-MS was performed on fresh kidney tissue (∼1-5 cm(3)), within 1 h of resection, from 21 human subjects afflicted by renal cell carcinoma (RCC). The preliminary diagnostic value of TS-MS data taken from freshly resected tissue was evaluated...
August 2016: Analytical and Bioanalytical Chemistry
Eugenio Alladio, Roberto Caruso, Enrico Gerace, Eleonora Amante, Alberto Salomone, Marco Vincenti
The Technical Document TD2014EAAS was drafted by the World Anti-Doping Agency (WADA) in order to fight the spread of endogenous anabolic androgenic steroids (EAAS) misuse in several sport disciplines. In particular, adoption of the so-called Athlete Biological Passport (ABP) - Steroidal Module allowed control laboratories to identify anomalous EAAS concentrations within the athletes' physiological urinary steroidal profile. Gas chromatography (GC) combined with mass spectrometry (MS), indicated by WADA as an appropriate technique to detect urinary EAAS, was utilized in the present study to develop and fully-validate an analytical method for the determination of all EAAS markers specified in TD2014EAAS, plus two further markers hypothetically useful to reveal microbial degradation of the sample...
May 30, 2016: Analytica Chimica Acta
Allison H Feibus, Oliver Sartor, Krishnarao Moparty, Kevin Chagin, Michael W Kattan, Elisa Ledet, Justin Levy, Benjamin Lee, Raju Thomas, Jonathan L Silberstein
PURPOSE: Prostate specific antigen has decreased performance characteristics for the detection of prostate cancer in African-American men. We evaluated urinary PCA3 and TMPRSS2:ERG in a racially diverse group of men. MATERIALS AND METHODS: After institutional review board approval, post-examination urine was prospectively collected before prostate biopsy. PCA3 and TMPRSS2:ERG RNA copies were quantified using transcription mediated amplification assays (Hologic, San Diego, California)...
October 2016: Journal of Urology
James McKiernan, Michael J Donovan, Vince O'Neill, Stefan Bentink, Mikkel Noerholm, Susan Belzer, Johan Skog, Michael W Kattan, Alan Partin, Gerald Andriole, Gordon Brown, John T Wei, Ian M Thompson, Peter Carroll
IMPORTANCE: Overdiagnosis and overtreatment of indolent prostate cancer (PCA) is a serious health issue in most developed countries. There is an unmet clinical need for noninvasive, easy to administer, diagnostic assays to help assess whether a prostate biopsy is warranted. OBJECTIVE: To determine the performance of a novel urine exosome gene expression assay (the ExoDx Prostate IntelliScore urine exosome assay) plus standard of care (SOC) (ie, prostate-specific antigen [PSA] level, age, race, and family history) vs SOC alone for discriminating between Gleason score (GS)7 and GS6 and benign disease on initial biopsy...
July 1, 2016: JAMA Oncology
Run Guo, Qunxing Tang, Yi Ye, Xiang Lu, Fan Chen, Xinhua Dai, Youyi Yan, Linchuan Liao
OBJECTIVE: The aim of this study was to examine whether or not there was a gender difference in CPP (conditioned placed preference) induced by ketamine and to further explore the effect of sex on metabolic responses to ketamine inducing in SD rats. METHODS: We measured ketamine-induced conditioned place preference and ketamine-induced metabolic changes in urine by using (1)H nuclear magnetic resonance (NMR) coupled with principal component analysis (PCA), partial least squares (PLS) and orthogonal signal correction (OSC) analysis...
June 2016: Regulatory Toxicology and Pharmacology: RTP
Prosper Kanyong, Sean Rawlinson, James Davis
Prostate cancer (PCa) is a significant cause of morbidity and mortality and the most common cancer in men in Europe, North America, and some parts of Africa. The established methods for detecting PCa are normally based on tests using Prostate Specific Antigen (PSA) in blood, Prostate cancer antigen 3 (PCA3) in urine and tissue Alpha-methylacyl-CoA racemase (AMACR) as tumour markers in patient samples. Prior to the introduction of PSA in clinics, prostatic acid phosphatase (PAP) was the most widely used biomarker...
2016: Journal of Cancer
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