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Gualtiero Alvisi, Marta Trevisan, Giulia Masi, Vanessa Canel, Luciana Caenazzo, Patrizia Nespeca, Luisa Barzon, Enzo Di Iorio, Vanessa Barbaro, Giorgio Palù
Human oral mucosa epithelial stem cells (hOMESCs) were obtained from a fresh oral biopsy collected from a healthy subject at the Fondazione Banca degli Occhi del Veneto (FBOV). An integration-free reprogramming protocol was applied exploiting episomal plasmids transfected into cells using a Nucleofector device. Around day 20 post transfection, several human induced pluripotent stem cell (hiPSC) colonies were manually picked and expanded. One of these (UNIPDi001-A-hiPSCs) expressed undifferentiated state marker alkaline phosphatase along with a panel of pluripotency state markers and was able to differentiate into the derivatives of all the three germ layers...
April 2018: Stem Cell Research
Alessandro Maloberti, Simone Maggioni, Lucia Occhi, Nicola Triglione, Francesco Panzeri, Stefano Nava, Stefano Signorini, Rosanna Falbo, Marco Casati, Guido Grassi, Cristina Giannattasio
Heterogeneous results have been obtained in the relationship between serum uric acid (SUA) and target organ damage (TOD) in patients with hypertension. Clinic blood pressure, SUA, and cardiac, arterial (carotid and aortic), and renal TOD were assessed in 762 consecutive patients with hypertension. Hyperuricemia was defined as an SUA >7.0 in men and >6.0 mg/dL in women. Men with hyperuricemia compared with those with normal SUA showed lower estimated glomerular filtration rates and E/A ratios and a higher prevalence of carotid plaques...
January 2018: Journal of Clinical Hypertension
Ingrid Gomes Perez Occhi-Alexandre, Mauro Luciano Baesso, Francielle Sato, Lidiane Vizioli de Castro-Hoshino, Pedro Luiz Rosalen, Raquel Sano Suga Terada, Antonio Medina Neto, Mitsue Fujimaki
BACKGROUND: Photodynamic therapy has a topical action, so it is important for the photosensitizer to penetrate into the tissue to which it has been applied to induce inactivation of the microorganisms. This study aimed to compare the penetration of two different concentrations of erythrosine into sound and in vitro decayed dentin samples. METHODS: This in vitro study evaluated erythrosine (0.3 and 5%) penetration into sound and decayed dentin. A total of 11 dentin discs were prepared and divided into two equal halves, in order to keep one half sound while the other half was submitted to sterilization and an in vitro demineralization model for 5 days...
November 20, 2017: Photodiagnosis and Photodynamic Therapy
Kalliopi Pilichou, Elisabetta Lazzarini, Ilaria Rigato, Rudy Celeghin, Marzia De Bortoli, Marina Perazzolo Marra, Marco Cason, Jan Jongbloed, Martina Calore, Stefania Rizzo, Daniela Regazzo, Giulia Poloni, Sabino Iliceto, Luciano Daliento, Pietro Delise, Domenico Corrado, J Peter Van Tintelen, Gaetano Thiene, Alessandra Rampazzo, Cristina Basso, Barbara Bauce, Alessandra Lorenzon, Gianluca Occhi
BACKGROUND: Arrhythmogenic cardiomyopathy (AC) is an inherited heart muscle disease associated with point mutations in genes encoding for cardiac desmosome proteins. Conventional mutation screening is positive in ≈50% of probands. Copy number variations (CNVs) have recently been linked to AC pointing to the need to determine the prevalence of CNVs in desmosomal genes and to evaluate disease penetrance by cosegregation analysis in family members. METHODS AND RESULTS: A total of 160 AC genotype-negative probands for 5 AC desmosomal genes by conventional mutation screening underwent multiplex ligation-dependent probe amplification...
October 2017: Circulation. Arrhythmia and Electrophysiology
D Regazzo, M Losa, N M Albiger, M R Terreni, G Vazza, F Ceccato, E Emanuelli, L Denaro, C Scaroni, G Occhi
OBJECTIVE: Glucose-dependent insulinotropic polypeptide receptor (GIPR) overexpression has been recently described in a proportion of gsp(-) somatotropinomas and suggested to be associated with the paradoxical increase of GH (GH-PI) during an oral glucose load. DESIGN AND METHODS: This study was aimed at linking the GIP/GIPR pathway to GH secretion in 25 somatotropinomas-derived primary cultures and correlating molecular with clinical features in acromegalic patients...
May 2017: European Journal of Endocrinology
J R S Vitule, A P L da Costa, F A Frehse, L A V Bezerra, T V T Occhi, V S Daga, A A Padial
No abstract text is available yet for this article.
April 2017: Journal of Fish Biology
N M Albiger, D Regazzo, B Rubin, A M Ferrara, S Rizzati, E Taschin, F Ceccato, G Arnaldi, F Pecori Giraldi, A Stigliano, L Cerquetti, F Grimaldi, E De Menis, M Boscaro, M Iacobone, G Occhi, C Scaroni
ARMC5 mutations have recently been identified as a common genetic cause of primary bilateral macronodular adrenal hyperplasia (PBMAH). We aimed to assess the prevalence of ARMC5 germline mutations and correlate genotype with phenotype in a large cohort of PBMAH patients. A multicenter study was performed, collecting patients from different endocrinology units in Italy. Seventy-one PBMAH patients were screened for small mutations and large rearrangements in the ARMC5 gene: 53 were cortisol-secreting (two with a family history of adrenal hyperplasia) and 18 were non-secreting cases of PBMAH...
March 2017: Endocrine
S Cannavo, M Ragonese, S Puglisi, P D Romeo, M L Torre, A Alibrandi, C Scaroni, G Occhi, F Ceccato, D Regazzo, E De Menis, P Sartorato, G Arnaldi, L Trementino, F Trimarchi, F Ferrau
In this multicentric study, we aimed to correlate the occurrence of AHR and/or AIP. genes variants in acromegalic patients with the disease severity and/or with the response to somatostatin analogs (SSa) treatment, according to pollution exposition.
April 2016: Journal of Clinical Endocrinology and Metabolism
Lei Guo, Xi Chen, Li-Na Li, Wei Tang, Yi-Ting Pan, Jian-Qiang Kong
BACKGROUND: (2S)-Pinocembrin is a chiral flavanone with versatile pharmacological and biological activities. Its health-promoting effects have spurred on research effects on the microbial production of (2S)-pinocembrin. However, an often-overlooked salient feature in the analysis of microbial (2S)-pinocembrin is its chirality. RESULTS: Here, we presented a full characterization of absolute configuration of microbial (2S)-pinocembrin from engineered Escherichia coli...
2016: Microbial Cell Factories
Francesco Ferraù, P D Romeo, S Puglisi, M Ragonese, M L Torre, C Scaroni, G Occhi, E De Menis, G Arnaldi, F Trimarchi, S Cannavò
This multicentric study aimed to investigate the prevalence of the G protein-coupled receptor 101 (GPR101) p.E308D variant and aryl hydrocarbon receptor interacting protein (AIP) gene mutations in a representative cohort of Italian patients with acromegaly. 215 patients with GH-secreting pituitary adenomas, referred to 4 Italian referral centres for pituitary diseases, have been included. Three cases of gigantism were present. Five cases were classified as FIPA. All the patients have been screened for germline AIP gene mutations and GPR101 gene p...
December 2016: Endocrine
Alessandra Lorenzon, Kalliopi Pilichou, Ilaria Rigato, Giovanni Vazza, Marzia De Bortoli, Martina Calore, Gianluca Occhi, Elisa Carturan, Elisabetta Lazzarini, Marco Cason, Elisa Mazzotti, Giulia Poloni, Maria Luisa Mostacciuolo, Luciano Daliento, Gaetano Thiene, Domenico Corrado, Cristina Basso, Barbara Bauce, Alessandra Rampazzo
Dominant mutations in desmocollin-2 (DSC2) gene cause arrhythmogenic cardiomyopathy (ACM), a progressive heart muscle disease characterized by ventricular tachyarrhythmias, heart failure, and risk of juvenile sudden death. Recessive mutations are rare and are associated with a cardiac or cardiocutaneous phenotype. Here, we evaluated the impact of a homozygous founder DSC2 mutation on clinical expression of ACM. An exon-by-exon analysis of the DSC2 coding region was performed in 94 ACM index patients. The c...
October 15, 2015: American Journal of Cardiology
N M Albiger, F Ceccato, M Zilio, M Barbot, G Occhi, S Rizzati, A Fassina, F Mantero, M Boscaro, M Iacobone, C Scaroni
CONTEXT: Bilateral macronodular adrenal hyperplasia (BMAH) is a rare form of Cushing's syndrome (CS). A variety of in vivo tests to identify aberrant receptor expression have been proposed to guide medical treatment. Unilateral adrenalectomy (UA) may be effective in selected patients, but little is known about recurrence during follow-up. OBJECTIVE: To describe a series of patients with BMAH and CS treated by different approaches, with a particular focus on the benefit of UA...
June 2015: Clinical Endocrinology
Filippo Ceccato, Giuseppe Lombardi, Renzo Manara, Enzo Emanuelli, Luca Denaro, Laura Milanese, Marina Paola Gardiman, Roberta Bertorelle, Massimo Scanarini, Domenico D'Avella, Gianluca Occhi, Marco Boscaro, Vittorina Zagonel, Carla Scaroni
Aggressive pituitary adenomas (PAs) are clinically challenging for endocrinologists and neurosurgeons due to their locally invasive nature and resistance to standard treatment (surgery, medical or radiotherapy). Two pituitary-directed drugs have recently been proposed: temozolomide (TMZ) for aggressive PA, and pasireotide for ACTH-secreting PA. We describe the experience of our multidisciplinary team of endocrinologists, neurosurgeons, neuroradiologists, oncologists, otolaryngologists and pathologists with TMZ and pasireotide treatment for aggressive PAs in terms of their radiological shrinkage and genetic features...
March 2015: Journal of Neuro-oncology
Caterina Mian, Filippo Ceccato, Susi Barollo, Sara Watutantrige-Fernando, Nora Albiger, Daniela Regazzo, Paola de Lazzari, Gianmaria Pennelli, Sandra Rotondi, Davide Nacamulli, Maria Rosa Pelizzo, Marie-Lise Jaffrain-Rea, Franco Grimaldi, Gianluca Occhi, Carla Scaroni
AIM: Acromegaly reportedly carries an increased risk of malignant and benign thyroid tumors, with a prevalence of thyroid cancer of around 3-7%. Germline mutations in the aryl-hydrocarbon receptor (AHR) interacting protein (AIP) have been identified in familial forms of acromegaly. The molecular and endocrine relationships between follicular thyroid growth and GH-secreting pituitary adenoma have yet to be fully established. Our aim was to study the prevalence of differentiated thyroid cancer (DTC) in acromegaly, focusing on the role of genetic events responsible for the onset of thyroid cancer...
2014: PloS One
Gianluca Occhi, Daniela Regazzo, Nora Maria Albiger, Filippo Ceccato, Sergio Ferasin, Massimo Scanarini, Luca Denaro, Chiara Cosma, Mario Plebani, Maria Francesca Cassarino, Giovanna Mantovani, Günter K Stalla, Francesca Pecori Giraldi, Marcelo Paez-Pareda, Carla Scaroni
Cushing's disease (CD) is a rare condition in which hypercortisolemia is secondary to excessive ACTH release from a pituitary corticotroph adenoma. CD is associated with significant morbidity and mortality, and a safe therapy that effectively targets the pituitary tumor is still lacking. Retinoic acid (RA) and dopamine agonists (DAs) have recently been considered as monotherapy in CD patients, and satisfactory results have been reported, albeit in a limited number of patients. Given the permissive role of RA on the dopamine receptor type-2 (DRD2), the aim of the present study was to see whether a combination of 9-cis RA and the DA bromocriptine (Br) might represent a possible treatment for CD...
September 2014: Endocrinology
Filippo Ceccato, Gianluca Occhi, Daniela Regazzo, Maria Luigia Randi, Diego Cecchin, Marina Paola Gardiman, Renzo Manara, Giuseppe Lombardi, Luca Denaro, Franco Mantero, Carla Scaroni
BACKGROUND: Most pituitary adenomas with FSH- or LH-positive immunohistochemistry are endocrinologically silent, and neurological symptoms due to their large volume are the first clinical signs; they are rarely reported to be secreting gonadotropins, this usually occurring in cases with clinical endocrine findings. Gonadotropinomas are often treated surgically because they are unresponsive to conventional medical therapies. Temozolomide was recently recommended for non-responder aggressive pituitary adenoma management...
January 2014: Hormones: International Journal of Endocrinology and Metabolism
No abstract text is available yet for this article.
October 1951: Revista Odontologica
No abstract text is available yet for this article.
April 1950: Revista Odontologica
Rune Thorsen, Davide Dalla Costa, Sara Chiaramonte, Luca Binda, Ettore Beghi, Tiziana Redaelli, Eugenio Occhi, Maurizio Ferrarin
OBJECTIVES: The primary purpose of this study was to evaluate myoelectrically controlled functional electrical stimulation (MeCFES) for enhancing the tenodesis grip in people with tetraplegia. The second aim was to estimate the potential number of candidates for the MeCFES device. The application of MeCFES provides the user with direct control of the grasp force as opposed to triggered FES systems. METHODS: Screening 253 medical records of C5 to C7 spinal cord injury resulted in 27 participants who trained activities of daily living for 12 × 2 hours, using the MeCFES...
2013: TheScientificWorldJournal
Marie-Lise Jaffrain-Rea, Sandra Rotondi, Annarita Turchi, Gianluca Occhi, Anne Barlier, Erika Peverelli, Lilya Rostomyan, Céline Defilles, Mariolina Angelini, Maria-Antonietta Oliva, Filippo Ceccato, Orlando Maiorani, Adrian F Daly, Vincenzo Esposito, Francesca Buttarelli, Dominique Figarella-Branger, Felice Giangaspero, Anna Spada, Carla Scaroni, Edoardo Alesse, Albert Beckers
Germline aryl hydrocarbon receptor interacting protein (AIP) gene mutations confer a predisposition to pituitary adenoma (PA), predominantly GH-secreting (GH-PA). As recent data suggest a role for AIP in the pathogenesis of sporadic GH-PA and their response to somatostatin analogues (SSA), the expression of AIP and its partner, aryl hydrocarbon receptor (AHR), was determined by semiquantitative immunohistochemistry scoring in 62 sporadic GH-PA (37 treated with SSA preoperatively). The influence of Gsp status was studied in a subset of tumours (n=39, 14 Gsp(+)) and six GH-PA were available for primary cultures...
October 2013: Endocrine-related Cancer
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