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https://www.readbyqxmd.com/read/28068152/effect-of-exposure-type-and-timing-of-injuries-in-division-i-college-football-a-4-year-single-program-analysis
#1
Michael K Krill, James R Borchers, Joshua T Hoffman, Rachel L Tatarski, Timothy E Hewett
BACKGROUND: Football players compete with a high risk of injury due to the sport. With the recent efforts to improve safety, the National Collegiate Athletic Association (NCAA) established new terminology to clearly define exposure types and reduce the number of high contact exposures. OBJECTIVES: To compare football injury rates (IR) with a focus on game versus practice, time in season of injury, mechanism of injury and utilizing recent exposure types defined by the NCAA (live contact, full-pads and non-contact)...
January 9, 2017: Physician and Sportsmedicine
https://www.readbyqxmd.com/read/28027326/haemophilus-ducreyi-cutaneous-ulcer-strains-diverged-from-both-class-i-and-class-ii-genital-ulcer-strains-implications-for-epidemiological-studies
#2
Dharanesh Gangaiah, Stanley M Spinola
BACKGROUND: Haemophilus ducreyi has emerged as a major cause of cutaneous ulcers (CU) in yaws-endemic regions of the tropics in the South Pacific, South East Asia and Africa. H. ducreyi was once thought only to cause the genital ulcer (GU) disease chancroid; GU strains belong to 2 distinct classes, class I and class II. Using whole-genome sequencing of 4 CU strains from Samoa, 1 from Vanuatu and 1 from Papua New Guinea, we showed that CU strains diverged from the class I strain 35000HP and that one CU strain expressed β-lactamase...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28008993/expanding-the-genetic-code-of-salmonella-with-non-canonical-amino-acids
#3
Qinglei Gan, Brent P Lehman, Thomas A Bobik, Chenguang Fan
The diversity of non-canonical amino acids (ncAAs) endows proteins with new features for a variety of biological studies and biotechnological applications. The genetic code expansion strategy, which co-translationally incorporates ncAAs into specific sites of target proteins, has been applied in many organisms. However, there have been only few studies on pathogens using genetic code expansion. Here, we introduce this technique into the human pathogen Salmonella by incorporating p-azido-phenylalanine, benzoyl-phenylalanine, acetyl-lysine, and phosphoserine into selected Salmonella proteins including a microcompartment shell protein (PduA), a type III secretion effector protein (SteA), and a metabolic enzyme (malate dehydrogenase), and demonstrate practical applications of genetic code expansion in protein labeling, photocrosslinking, and post-translational modification studies in Salmonella...
December 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28005635/longitudinal-body-composition-changes-in-ncaa-division-i-college-football-players
#4
Eric T Trexler, Abbie E Smith-Ryan, J Bryan Mann, Pat A Ivey, Katie R Hirsch, Meredith G Mock
Trexler, ET, Smith-Ryan, AE, Mann, JB, Ivey, PA, Hirsch, KR, and Mock, MG. Longitudinal body composition changes in NCAA Division I college football players. J Strength Cond Res 31(1): 1-8, 2017-Many athletes seek to optimize body composition to fit the physical demands of their sport. American football requires a unique combination of size, speed, and power. The purpose of the current study was to evaluate longitudinal changes in body composition in Division I collegiate football players. For 57 players (mean ± SD, age = 19...
January 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/27999720/changes-in-dynamic-balance-and-hip-strength-after-an-eight-week-conditioning-program-in-ncaa-division-i-female-soccer-football-athletes
#5
Brandon M Ness, Brett A Comstock, William E Schweinle
BACKGROUND: Lower extremity injury commonly affects female soccer athletes. Decreased dynamic balance and hip strength are identified risk factors for lower extremity injury. Little is known about how these factors adapt to a training stimulus in this population. PURPOSE: To retrospectively investigate changes in lower extremity dynamic balance and isometric hip strength in Division I collegiate female soccer athletes after participating in an eight-week strength and conditioning program...
December 2016: International Journal of Sports Physical Therapy
https://www.readbyqxmd.com/read/27996092/maxillofacial-injuries-among-national-collegiate-athletic-association-ncaa-athletes-2004-to-2014
#6
Stephen R Chorney, Lindsay Sobin, Parul Goyal, Amar C Suryadevara
OBJECTIVE: Participation in National Collegiate Athletic Association (NCAA) sports increases annually, yet the risk of maxillofacial injuries among these athletes is unknown. We report the incidence and trends in maxillofacial injuries among NCAA athletes. STUDY DESIGN: Retrospective study of the NCAA Injury Surveillance System (ISS) representing athletes from seven men's and eight women's sports across Divisions 1, 2, and 3. Incidence of maxillofacial injuries by sport, gender, anatomic location, and injuries requiring surgery were measured...
December 20, 2016: Laryngoscope
https://www.readbyqxmd.com/read/27987323/translation-system-engineering-in-escherichia-coli-enhances-non-canonical-amino-acid-incorporation-into-proteins
#7
Rui Gan, Jessica G Perez, Erik D Carlson, Ioanna Ntai, Farren J Isaacs, Neil L Kelleher, Michael C Jewett
The ability to site-specifically incorporate noncanonical amino acids (ncAAs) into proteins has made possible the study of protein structure and function in fundamentally new ways, as well as the synthesis of unnatural polymers. However, the task of site-specifically incorporating multiple ncAAs into proteins with high purity and yield continues to present a challenge. At the heart of this challenge lies the lower efficiency of engineered orthogonal translation system components compared to their natural counterparts (e...
December 17, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27938668/cellular-encoding-of-cy-dyes-for-single-molecule-imaging
#8
Lilia Leisle, Rahul Chadda, John D Lueck, Daniel T Infield, Jason D Galpin, Venkatramanan Krishnamani, Janice L Robertson, Christopher A Ahern
A general method is described for the site-specific genetic encoding of cyanine dyes as non-canonical amino acids (Cy-ncAAs) into proteins. The approach relies on an improved technique for nonsense suppression with in vitro misacylated orthogonal tRNA. The data show that Cy-ncAAs (based on Cy3 and Cy5) are tolerated by the eukaryotic ribosome in cell-free and whole-cell environments and can be incorporated into soluble and membrane proteins. In the context of the Xenopus laevis oocyte expression system, this technique yields ion channels with encoded Cy-ncAAs that are trafficked to the plasma membrane where they display robust function and distinct fluorescent signals as detected by TIRF microscopy...
December 12, 2016: ELife
https://www.readbyqxmd.com/read/27932242/elevated-salivary-iga-decreased-anxiety-and-an-altered-oral-microbiota-are-associated-with-active-participation-on-an-undergraduate-athletic-team
#9
Ashley L Lamb, Debra E Hess, Sherie Edenborn, Elizabeth Ubinger, Andres E Carrillo, Pierette M Appasamy
BACKGROUND: Previous reports indicate that regular, but not excessive, exercise can moderate the response to anxiety and alter the immune response, therefore we hypothesized that college student athletes who were actively participating on an NCAA Division III athletics team ("in-season") would have lower levels of anxiety and higher salivary IgA levels than similar college athletes who were in their "off-season". NCAA Division III athletes participate in athletics at a level of intensity that is more moderate compared to other NCAA divisions...
February 1, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/27930454/fat-free-mass-index-in-ncaa-division-i-and-ii-collegiate-american-football-players
#10
Eric T Trexler, Abbie E Smith-Ryan, Malia N M Blue, Richard M Schumacher, Jerry L Mayhew, J Bryan Mann, Pat A Ivey, Katie R Hirsch, Meredith G Mock
Fat-free mass index (FFMI) is a height-adjusted assessment of fat-free mass, with previous research suggesting a natural upper limit of 25 kg[BULLET OPERATOR]m in resistance-trained males. The current study evaluated upper limits for FFMI in collegiate American football players (n=235), and evaluated differences between positions, divisions, and age groups. The sample consisted of two NCAA Division I teams (n=78, n=69), and one Division II team (n=88). Body composition was assessed via dual-energy x-ray absorptiometry and used to calculate FFMI; linear regression was used to normalize values to a height of 180 cm...
November 19, 2016: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/27926615/coronary-artery-evaluation-by-screening-echocardiogram-in-intercollegiate-athletes
#11
Walter J Hoyt, Peter N Dean, Daniel S Schneider, Mark R Conaway, Christopher M Kramer, Robert W Battle
INTRODUCTION: Coronary artery anomalies are the second most common congenital cause of sudden cardiac death (SCD) in young athletes. Some centers have advocated for limited transthoracic echocardiogram (TTE) protocols in the screening of college athletes, which may miss important causes of SCD. PURPOSE: The purpose of this study is to evaluate the ability of screening TTE to determine origin and proximal course of the coronary arteries in intercollegiate athletes...
December 6, 2016: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/27919800/increasing-the-fidelity-of-noncanonical-amino-acid-incorporation-in-cell-free-protein-synthesis
#12
Qinglei Gan, Chenguang Fan
BACKGROUND: Cell-free protein synthesis provides a robust platform for co-translational incorporation of noncanonical amino acid (ncAA) into proteins to facilitate biological studies and biotechnological applications. Recently, eliminating the activity of release factor 1 has been shown to increase ncAA incorporation in response to amber codons. However, this approach could promote mis-incorporation of canonical amino acids by near cognate suppression. METHODS: We performed a facile protocol to remove near cognate tRNA isoacceptors of the amber codon from total tRNAs, and used the phosphoserine (Sep) incorporation system as validation...
December 2, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27893262/reducing-barriers-to-mental-health-care-for-student-athletes-an-integrated-care-model
#13
Laura E Sudano, Greg Collins, Christopher M Miles
Research suggests that National Collegiate Athletic Association (NCAA) Division I student-athletes have higher levels of stress and other behavioral health issues, including substance use, than nonathletes. For several reasons, student-athletes may be less likely to admit to behavioral health issues and seek mental health care. Integrated care is a model of care that integrates behavioral health into a medical practice. This article explores the newly released NCAA Best Mental Health Practice guidelines and the application of integrated care to a Division I athletic training room setting using the three-worldview framework for successful integration, incorporating clinical outcomes, operational reliability, and financial stability...
November 28, 2016: Families, Systems & Health: the Journal of Collaborative Family Healthcare
https://www.readbyqxmd.com/read/27879169/goal-orientation-and-well-being-in-college-athletes-the-importance-of-athletic-social-connectedness
#14
Heidi A Wayment, Andrew Schrack Walters
The present study examined the ability of an interpersonal construct called athletic connectedness to mediate the relationship between task and ego goal orientations and well-being. We operationalised athletic social connectedness as a sense of social belonging and sense of connection with teammates. We hypothesised that athletic social connectedness would be positively associated with task goals, negatively associated with ego goals, and would at least partially mediate the relationship between achievement goals and well-being...
November 23, 2016: Journal of Sports Sciences
https://www.readbyqxmd.com/read/27873542/a-6-year-surveillance-study-of-stingers-in-ncaa-american-football
#15
James Green, Scott L Zuckerman, Sara L Dalton, Aristarque Djoko, Dustin Folger, Zachary Y Kerr
This study describes the epidemiology of "stinger" injuries in National Collegiate Athletic Association (NCAA) Men's Football. About 57 NCAA Men's Football programmes provided 153 team-seasons of injury data to the NCAA Injury Surveillance Programme (NCAA-ISP) during the 2009/2010-2014/2015 academic years. In the study period, 229 "stingers" were reported for an injury rate of 2.04/10,000 athlete-exposures (AE). Most "stingers" were reported during competitions (55.5%) and the preseason (80.3%) and resulted in time loss less than 24 hours (63...
January 2017: Research in Sports Medicine
https://www.readbyqxmd.com/read/27805363/nitrilase-activatable-noncanonical-amino-acid-precursors-for-cell-selective-metabolic-labeling-of-proteomes
#16
Zefan Li, Yuntao Zhu, Yuting Sun, Ke Qin, Weibing Liu, Wen Zhou, Xing Chen
Cell-selective protein metabolic labeling is of great interest for studying cell-cell communications and tissue homeostasis. We herein describe a nitrilase-activatable noncanonical amino acid tagging (NANCAT) strategy that exploits an exogenous nitrilase to enzymatically convert the nitrile-substituted precursors to their corresponding noncanonical amino acids (ncAAs), l-azidohomoalanine (Aha) or homopropargylglycine (Hpg), in living cells. Only cells expressing the nitrilase can generate Aha or Hpg in cellulo and metabolically incorporate them into the nascent proteins...
December 16, 2016: ACS Chemical Biology
https://www.readbyqxmd.com/read/27804198/debugging-eukaryotic-genetic-code-expansion-for-site-specific-click-paint-super-resolution-microscopy
#17
Ivana Nikić, Gemma Estrada Girona, Jun Hee Kang, Giulia Paci, Sofya Mikhaleva, Christine Koehler, Nataliia V Shymanska, Camilla Ventura Santos, Daniel Spitz, Edward A Lemke
Super-resolution microscopy (SRM) greatly benefits from the ability to install small photostable fluorescent labels into proteins. Genetic code expansion (GCE) technology addresses this demand, allowing the introduction of small labeling sites, in the form of uniquely reactive noncanonical amino acids (ncAAs), at any residue in a target protein. However, low incorporation efficiency of ncAAs and high background fluorescence limit its current SRM applications. Redirecting the subcellular localization of the pyrrolysine-based GCE system for click chemistry, combined with DNA-PAINT microscopy, enables the visualization of even low-abundance proteins inside mammalian cells...
December 23, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27802962/incidence-and-epidemiology-of-foot-and-ankle-injuries-in-elite-collegiate-athletes
#18
Kenneth J Hunt, Daniel Hurwit, Kevin Robell, Corey Gatewood, Itamar B Botser, Gordon Matheson
BACKGROUND: Foot and ankle injuries are increasing in competitive professional and collegiate athletics. Many of these injuries result in considerable missed time from sports and often require surgical intervention. To develop and implement effective practice participation strategies, return-to-play protocols, and injury prevention programs, an understanding of injury trends and epidemiology is vital. PURPOSE: This study aimed to evaluate the incidence of foot and ankle injuries in elite athletes participating in 37 sports at a single National Collegiate Athletics Association (NCAA) Division 1 institution...
November 1, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27787466/relationship-between-running-performance-and-recovery-stress-state-in-collegiate-soccer-players
#19
Nicholas A Coker, Klarie M Ake, David L Griffin, Stephen J Rossi, Jim L McMillan, Adam J Wells
The purpose of this study was to evaluate the relationship between changes in running performance and the stress-recovery state in collegiate soccer players. Running performance was evaluated in seven Division I NCAA male soccer players (179.39±5.24 cm; 75.46±5.98 kg; 20.37±1.41 yrs.) via global positioning system over the course of 12 competitive games in a single season. The regular season was divided into four competitive blocks: B1 (n=3), B2 (n=3), B3 (n=3) and B4 (n=3). Total distance and distance covered while engaging in walking, jogging, low-speed running, high-speed running, sprinting, low-intensity running and high-intensity running were assessed during each block...
October 17, 2016: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/27783132/orthogonal-protein-translation-using-pyrrolysyl-trna-synthetases-for-single-and-multiple-noncanonical-amino-acid-mutagenesis
#20
Tobias Baumann, Matthias Exner, Nediljko Budisa
To date, the two systems most extensively used for noncanonical amino acid (ncAA) incorporation via orthogonal translation are based on the Methanococcus jannaschii TyrRS/tRNA CUA(Tyr) and the Methanosarcina barkeri/Methanosarcina mazei PylRS/tRNA CUA(Pyl) pairs. Here, we summarize the development and usage of the pyrrolysine-based system for orthogonal translation, a process that allows for the recombinant production of site-specifically labeled proteins and peptides. Via stop codon suppression in Escherichia coli and mammalian cells, genetically encoded biomolecules can be equipped with a great diversity of chemical functionalities including click chemistry handles, post-translational modifications, and photocaged sidechains...
October 26, 2016: Advances in Biochemical Engineering/biotechnology
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