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Yahya Homsi, Thorsten Lang
Tetraspanins are cell membrane-scaffolding proteins interacting with one another and a repertoire of interaction partners. Through these interactions, they form extended molecular networks as tetraspanin webs or tetraspanin-enriched microdomains. Microscopic data suggest that these networks contain tetraspanin clusters, with poor overlap between clusters formed by different tetraspanins. Here, we investigate the possibility of targeting tetraspanins CD9 or CD151 to clusters formed by the tetraspanin CD81. We find that the δ-loop from the large extracellular domain of CD81 is sufficient for targeting of CD9/CD151 to CD81 clusters...
February 2017: FEBS Open Bio
Jessica Yu, Chia-Ying Lee, Chun Austin Changou, Dora M Cedano-Prieto, Yoko K Takada, Yoshikazu Takada
Tetraspanins play important roles in normal (e.g., cell adhesion, motility, activation, and proliferation), and pathological conditions (e.g., metastasis and viral infection). Tetraspanins interact with integrins and regulate integrin functions, but the specifics of tetraspanin-integrin interaction are unclear. Using co-immunoprecipitation with integrins as a sole method to detect interaction between integrins and full-length tetraspanins, it has been proposed that the variable region (helices D and E) of the extracellular-2 (EC2) of tetraspanins laterally associate with non-ligand-binding site of integrins...
December 19, 2016: Biochemical Journal
Rosaliana Libro, Domenico Scionti, Francesca Diomede, Marco Marchisio, Gianpaolo Grassi, Federica Pollastro, Adriano Piattelli, Placido Bramanti, Emanuela Mazzon, Oriana Trubiani
Human Gingival Mesenchymal Stem Cells (hGMSCs) are multipotential cells that can expand and differentiate in culture under specific and standardized conditions. In the present study, we have investigated whether in vitro pre-treatment of hGMSCs with Cannabidiol (CBD) can influence their expression profile, improving the therapeutic potential of this cell culture. Following CBD treatment (5 μM) for 24 h, gene expression analysis through Next Generation Sequencing (NGS) has revealed several genes differentially expressed between CBD-treated hGMSCs (CBD-hGMSCs) and control cells (CTR-hGMSCs) that were linked to inflammation and apoptosis...
2016: Frontiers in Physiology
Ping Zeng, Yin-Hua Wang, Meng Si, Jin-Hua Gu, Ping Li, Pei-Hua Lu, Min-Bin Chen
Tetraspanin CD151, also known as PETA-3 or SFA-1, has been reported to predict prognosis in various solid tumors. Yet, the results of these studies remained inconclusive. Here, we performed this meta-analysis of relevant studies published on the topic to quantitatively evaluate the clinicopathological significance of CD151 in solid tumors. The relevant articles were identified via searching the PubMed, Web of Science and Embase database. The pooled hazard ratios (HRs) and corresponding 95% confidence intervals (CI) of overall survival (OS) and disease-free survival (DFS) were calculated to evaluate the prognostic value of CD151 expression in patients with solid tumors...
November 23, 2016: Oncotarget
Ji Hye Kim, Mohamed Badawi, Jong-Kook Park, Jinmai Jiang, Xiaokui Mo, Lewis R Roberts, Thomas D Schmittgen
Several studies have reported reduced miR-199a-3p expression in hepatocellular carcinoma (HCC). In an effort to discover important target genes for miR-199a-3p that may be related to HCC development or progression, we identified the tetraspanin, transmembrane glycoprotein CD151. Luciferase reporter assays and western blotting identified CD151 as a bona fide miR-199a-3p target gene. While CD151 protein was increased in the mesenchymal but not the epithelial HCC cell lines, CD151 knockdown with siRNA did not reduce HCC cell proliferation in either group of cells...
November 2016: International Journal of Oncology
Oliver M Fisher, Angelique J Levert-Mignon, Christopher W Lehane, Natalia K Botelho, Jesper L V Maag, Melissa L Thomas, Melanie Edwards, Sarah J Lord, Yuri V Bobryshev, David C Whiteman, Reginald V Lord
BACKGROUND: Esophageal and gastroesophageal junctional (GEJ) adenocarcinoma is one of the most fatal cancers and has the fastest rising incidence rate of all cancers. Identification of biomarkers is needed to tailor treatments to each patient's tumor biology and prognosis. METHODS: Gene expression profiling was performed in a test cohort of 80 chemoradiotherapy (CRTx)-naïve patients with external validation in a separate cohort of 62 CRTx-naïve patients and 169 patients with advanced-stage disease treated with CRTx...
December 2016: Annals of Surgical Oncology
Xiaosheng Yang, Shiting Li, Jun Zhong, Wenchuan Zhang, Xuming Hua, Bin Li, Hui Sun
Crosstalk between the nervous and vascular systems is important during development and in response to injury, and the laminin-like axonal guidance protein netrin-1 has been studied for its involvement in angiogenesis and vascular remodelling. In this study, we examined the role of netrin-1 in angiogenesis and explored the underlying mechanisms. The effect of netrin-1 on brain tissues and endothelial cells was examined by immunohistochemistry and western blotting in a middle cerebral artery occlusion model and in human umbilical vein endothelial cells...
January 2017: Journal of Cellular and Molecular Medicine
Zhuoying Wang, Chongren Wang, Zifei Zhou, Mengxiong Sun, Chenghao Zhou, Jian Chen, Fei Yin, Hongsheng Wang, Binhui Lin, Dongqing Zuo, Suoyuan Li, Lijin Feng, Zhenfeng Duan, Zhengdong Cai, Yingqi Hua
CD151, a tetraspanin family protein involved in cell-cell and cell-extracellular matrix interaction, is differentially expressed in osteosarcoma cell membranes. Thus, this study aimed to investigate the role of CD151 in osteosarcoma metastasis. We analyzed CD151 expression in patient tissue samples using immunohistochemistry. CD151 expression was also silenced with shRNA in osteosarcoma cells of high metastatic potential, and cell adhesion, migration and invasion were evaluated in vitro and pulmonary metastasis was investigated in vivo...
September 13, 2016: Oncotarget
Shao-Ling Yang, Ke-Qiang Tang, Jun-Jia Tao, Ai-Hong Wan, Yan-Duan Lin, Shu-Liang Nan, Qing-Kui Guo, Zhi Yong Shen, Bin Hu
OBJECTIVES: We aimed to evaluate whether ultrasound (US) and microbubble-mediated delivery of Cluster of Differentiation 151 (CD151) could enhance the therapeutic effects of CD151 on myocardial infarction (MI). METHODS: A rabbit model of MI was established by a modified Fujita method. Then, 50 MI rabbits were randomly divided into 5 groups, including G1 (CD151 plasmid and physiological saline in the presence of US); G2 (CD151 and Sonovue in the presence of US); G3 (CD151 and Sonovue in the absence of US); G4 (Sonovue in the absence of US), and a control group (physiological saline in the absence of US)...
August 13, 2016: Cardiology
Julie C Nienstedt, Alexander Gröbe, Patrick Lebok, Franziska Büscheck, Till Clauditz, Ronald Simon, Asmus Heumann, Guido Sauter, Christoph Moebius, Adrian Münscher, Rainald Knecht, Marco Blessmann, Max Heiland, Christina Pflug
OBJECTIVES: CD151 is a plasma membrane protein belonging to the tetraspanin family. CD151 represents a putative therapeutic target and has been suggested as a prognostic marker in several cancer types. The present study aims to investigate the prognostic relevance of immunohistochemical CD151 expression in head and neck squamous cell carcinoma (HNSCC). MATERIALS AND METHODS: Tissue microarray (TMA) sections containing samples from 667 cancers of oral cavity, oro- and hypopharynx and larynx, for which follow-up data were available, were analyzed for CD151 expression by immunohistochemistry...
July 21, 2016: Clinical Oral Investigations
Thale Kristin Olsen, Ioannis Panagopoulos, Ludmila Gorunova, Francesca Micci, Kristin Andersen, Hege Kilen Andersen, Torstein R Meling, Bernt Due-Tønnessen, David Scheie, Sverre Heim, Petter Brandal
We have previously identified two ALK rearrangements in a subset of ependymal tumors using a combination of cytogenetic data and RNA sequencing. The aim of the present study was to perform an unbiased search for fusion transcripts in our entire series of ependymal tumors. Fusion analysis was performed using the FusionCatcher algorithm on 12 RNA-sequenced ependymal tumors. Candidate transcripts were prioritized based on the software's filtering and manual visualization using the BLAST (Basic Local Alignment Search Tool) and BLAT (BLAST-like alignment tool) tools...
July 12, 2016: Genes, Chromosomes & Cancer
Birgitte Sandfeld-Paulsen, Kristine Raaby Jakobsen, Rikke Bæk, Birgitte Holst Folkersen, Torben Riis Rasmussen, Peter Meldgaard, Kim Varming, Malene Møller Jørgensen, Boe Sandahl Sorensen
INTRODUCTION: Exosomes have been suggested as promising biomarkers in NSCLC because they contain proteins from their originating cells and are readily available in plasma. In this study, we explored the potential of exosome protein profiling in diagnosing lung cancers of all stages and various histological subtypes in patients. METHODS: Plasma was isolated from 581 patients (431 with lung cancer and 150 controls). The extracellular vesicle array was used to phenotype exosomes...
October 2016: Journal of Thoracic Oncology
Tao Liu, Cai-Hua Zu, Shu-Sen Wang, Hong-Li Song, Zheng-Lu Wang, Xin-Nv Xu, Hong-Sheng Liu, Yu-Liang Wang, Zhong-Yang Shen
Competing endogenous RNAs (ceRNAs) are RNA transcripts that can crosstalk with each other by competing for shared microRNAs (miRNAs) through miRNA response elements (MREs). Involved in ceRNA networks, the RNA transcripts may be in a balance, disruption of which could lead to tumorigenesis. Here we reveal a ceRNA interaction between PIK3C2A and CD151 mRNAs in hepatocellular carcinoma (HCC) cells. PIK3C2A is a candidate ceRNA of CD151 because mRNA 3' untranslated regions (3'UTRs) of these two genes contain miR-124 binding sites...
July 12, 2016: Oncotarget
Ivana Halova, Petr Draber
The plasma membrane contains diverse and specialized membrane domains, which include tetraspanin-enriched domains (TEMs) and transmembrane adaptor protein (TRAP)-enriched domains. Recent biophysical, microscopic, and functional studies indicated that TEMs and TRAP-enriched domains are involved in compartmentalization of physicochemical events of such important processes as immunoreceptor signal transduction and chemotaxis. Moreover, there is evidence of a cross-talk between TEMs and TRAP-enriched domains. In this review we discuss the presence and function of such domains and their crosstalk using mast cells as a model...
2016: Frontiers in Cell and Developmental Biology
Yongkang Qiao, John Kit Chung Tam, Sheryl S L Tan, Yee Kit Tai, Chin Yein Chin, Alastair G Stewart, Leonie Ashman, Kiyotoshi Sekiguchi, Shenna Y Langenbach, Gerald Stelmack, Andrew J Halayko, Thai Tran
BACKGROUND: Airway smooth muscle (ASM) contraction underpins airway constriction; however, underlying mechanisms for airway hyperresponsiveness (AHR) remain incompletely defined. CD151, a 4-transmembrane glycoprotein that associates with laminin-binding integrins, is highly expressed in the human lung. The role of CD151 in ASM function and its relationship to asthma have yet to be elucidated. OBJECTIVE: We sought to ascertain whether CD151 expression is clinically relevant to asthma and whether CD151 expression affects AHR...
April 27, 2016: Journal of Allergy and Clinical Immunology
Linlin Zhang, Zhengzhi Cui, Lei Zhou, Youmin Kang, Li Li, Jinxiu Li, Yunping Dai, Shuyang Yu, Ning Li
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most devastating pathogens in the swine industry worldwide. Due to the lack of robust cell lines and small animal models, the pathogenesis of PRRSV infection and mechanism for protective vaccination are still not yet well understood. To obtain useful cell lines, several groups have attempted to construct different transgenic cell lines with three PRRSV receptors: CD163, CD169, and CD151. The results showed that CD163 is essential for PRRSV entry into target cells and replication, and both CD169 and CD151 play key roles during PRRSV infection...
2016: PloS One
Daniel Hochdorfer, Luise Florin, Christian Sinzger, Diana Lieber
UNLABELLED: Human cytomegalovirus (HCMV), a betaherpesvirus, can cause life-threatening disease in immunocompromised individuals. Viral envelope glycoproteins that mediate binding to and penetration into target cells have been identified previously. In contrast, cellular proteins supporting HCMV during entry are largely unknown. In order to systematically identify host genes affecting initial steps of HCMV infection, a targeted RNA interference screen of 96 cellular genes was performed in endothelial cells by use of a virus strain expressing the full set of known glycoprotein H and L (gH/gL) complexes...
July 15, 2016: Journal of Virology
Elena Stocco, Silvia Barbon, Paolo Radossi, Senthilkumar Rajendran, Daniele Dalzoppo, Marina Bortolami, Andrea Bagno, Francesca Grandi, Pier Giorgio Gamba, Pier Paolo Parnigotto, Giuseppe Tagariello, Claudio Grandi
Haemophilic arthropathy is the major cause of disability in patients with haemophilia and, despite prophylaxis with coagulation factor concentrates, some patients still develop articular complications. We evaluate the feasibility of a tissue engineering approach to improve current clinical strategies for cartilage regeneration in haemophiliacs by using autologous chondrocytes (haemophilic chondrocytes; HaeCs). Little is known about articular chondrocytes from haemophilic patients and no characterisation has as yet been performed...
October 2016: Cell and Tissue Research
Rachel L Stewart, Dava West, Chi Wang, Heidi L Weiss, Tamas Gal, Eric B Durbin, William O'Connor, Min Chen, Kathleen L O'Connor
Lung cancer carries a poor prognosis and is the most common cause of cancer-related death worldwide. The integrin α6β4, a laminin receptor, promotes carcinoma progression in part by cooperating with various growth factor receptors to facilitate invasion and metastasis. In carcinoma cells with mutant TP53, the integrin α6β4 promotes cell survival. TP53 mutations and integrin α6β4 overexpression co-occur in many aggressive malignancies. Because of the high frequency of TP53 mutations in lung squamous cell carcinoma (SCC), we sought to investigate the association of integrin β4 expression with clinicopathologic features and survival in non-small cell lung cancer (NSCLC)...
August 2016: Human Pathology
Jessica Tilghman, Paula Schiapparelli, Bachuchu Lal, Mingyao Ying, Alfredo Quinones-Hinojosa, Shuli Xia, John Laterra
Glioblastoma (GBM) stem cells (GSCs) represent tumor-propagating cells with stem-like characteristics (stemness) that contribute disproportionately to GBM drug resistance and tumor recurrence. Understanding the mechanisms supporting GSC stemness is important for developing therapeutic strategies for targeting GSC-dependent oncogenic mechanisms. Using GBM-derived neurospheres, we identified the cell surface tetraspanin family member CD151 as a novel regulator of glioma cell stemness, GSC self-renewal capacity, migration, and tumor growth...
March 2016: Neoplasia: An International Journal for Oncology Research
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