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https://www.readbyqxmd.com/read/28330219/effect-of-chlorpyrifos-on-soil-microbial-diversity-and-its-biotransformation-by-streptomyces-sp-hp-11
#1
M Supreeth, M A Chandrashekar, N Sachin, N S Raju
The application of pesticides in agricultural fields not only reaches the target pests but also with soil where it interacts with soil microorganisms resulting in change of microbial diversity. Chlorpyrifos (CP) is one such organophosphorous insecticide most widely used against various insects, termites, and beetles throughout the globe. In the present work, the effect of CP on soil microbial population was assessed by the cultivable method. The fertile soil which does not have a history of any pesticide application was treated with 100 and 200 µg/g of CP along with control which received only sterile water and incubated for 1, 7, and 14 days...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330177/pseudomonas-extremorientalis-bu118-a-new-salt-tolerant-laccase-secreting-bacterium-with-biotechnological-potential-in-textile-azo-dye-decolourization
#2
Mohamed Neifar, Habib Chouchane, Mouna Mahjoubi, Atef Jaouani, Ameur Cherif
The present investigation focused on screening of a new potent strain for laccase production and optimizing the process parameters to achieve the maximum enzymatic decolourization of textile azo dye Congo red. Seven hydrocarbonoclastic bacterial strains were selected as positive in laccase production in solid medium using 2,6 dimethoxyphenol as an enzyme activity indicator. The best enzyme producer Pseudomonas extremorientalis BU118 showed a maximum laccase activity of about 7000 U/L of wheat bran under solid-state conditions...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330094/characterization-of-antibiotic-resistant-and-enzyme-producing-bacterial-strains-isolated-from-the-arabian-sea
#3
Preeti N Tallur, Dayanand B Sajjan, Sikandar I Mulla, Manjunatha P Talwar, A Pragasam, Vinayak M Nayak, Harichandra Z Ninnekar, Shivanand S Bhat
Marine bacteria are known to produce many bioactive molecules and extracellular enzymes of commercial importance. We have investigated the bacterial diversity of the coastal area of Karwar, Karnataka State, India. Among these bacterial isolates, five bacterial strains were selected and identified by their morphological, biochemical characteristics and phylogenetic analysis based on 16S rRNA gene sequences. The identified bacterial isolates, Bacillus toyonensis PNTB1, Lysinibacillus sphaericus PTB, Vibrio vulnificus PMD, Shewanella MPTDBS, and Pseudomonas chlororaphis PNTB were characterized for their tolerance to salt and antibiotics...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28328973/differing-metabolic-responses-to-salt-stress-in-wheat-barley-addition-lines-containing-different-7h-chromosomal-fragments
#4
Eva Darko, Krisztián Gierczik, Orsolya Hudák, Péter Forgó, Magda Pál, Edina Türkösi, Viktória Kovács, Sándor Dulai, Imre Majláth, István Molnár, Tibor Janda, Márta Molnár-Láng
Salinity-induced osmotic, ionic and oxidative stress responses were investigated on Asakaze/Manas wheat/barley addition lines 7H, 7HL and 7HS, together with their barley (salt-tolerant) and wheat (relatively salt-sensitive) parents. Growth, photosynthetic activity, chlorophyll degradation, proline, glycine betaine accumulation, sugar metabolism, Na+ and K+ uptake and transport processes and the role of polyamines and antioxidants were studied in young plants grown in hydroponic culture with or without salt treatment...
2017: PloS One
https://www.readbyqxmd.com/read/28322088/alpha-tocopherol-dependent-salt-tolerance-is-more-related-with-auxin-synthesis-rather-than-enhancement-antioxidant-defense-in-soybean-roots
#5
Seda Sereflioglu, Burcu Seckin Dinler, Eda Tasci
In this paper, we describe the alleviated effects of alpha-tocopherol (α-T) on oxidative damage and its possible role as a signal transmitter in plants during salt stress. The results show that exogenously applied α-T under salt stress increased root length and weight, but reduced hydrogen peroxide (H2O2), superoxide anion radical (O2(.)-) and malondialdehyde (MDA) content in soybean roots. The proline content was reduced by α-T treatment. Interestingly, endogenous auxin (IAA) level was significantly increased after α-T application as compared to salt stress alone...
March 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28321231/jcdreb2-a-physic-nut-ap2-erf-gene-alters-plant-growth-and-salinity-stress-responses-in-transgenic-rice
#6
Yuehui Tang, Kun Liu, Ju Zhang, Xiaoli Li, Kedong Xu, Yi Zhang, Jing Qi, Deshui Yu, Jian Wang, Chengwei Li
Transcription factors of the AP2/ERF family play important roles in plant growth, development, and responses to biotic and abiotic stresses. In this study, a physic nut AP2/ERF gene, JcDREB2, was functionally characterized. Real-time PCR analysis revealed that JcDREB2 was expressed mainly in the leaf and could be induced by abscisic acid but suppressed by gibberellin (GA) and salt. Transient expression of a JcDREB2-YFP fusion protein in Arabidopsis protoplasts cells suggested that JcDREB2 is localized in the nucleus...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28319861/different-salt-concentrations-induce-alterations-both-in-photosynthetic-parameters-and-salt-gland-activity-in-leaves-of-the-mangrove-avicennia-schaueriana
#7
Janaina Dos Santos Garcia, Ândrea Carla Dalmolin, Marcel Giovanni Costa França, Pedro Antônio Oliveira Mangabeira
Mangrove sites are constantly under tidal regimes, where there is variation of dissolved oxygen and saline content in water. This study evaluated the effects of varying salinity in Avicennia schaueriana, an abundant species in the sea-mangrove-river interface. Seedlings of A. schaueriana were harvested at the riverbank and placed in different saline and nutrient solutions in a greenhouse in order to simulate a saline flow. After 7 acclimatization days, plants were exposed to the following saline concentrations: 0, 50, 170 and 250mM NaCl, for a period of 30 days...
March 16, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28315371/complete-genome-sequence-of-arthrobacter-sp-zxy-2-associated-with-effective-atrazine-degradation-and-salt-adaptation
#8
Xinyue Zhao, Fang Ma, Cuijie Feng, Shunwen Bai, Jixian Yang, Li Wang
An atrazine-degrading strain Arthrobacter sp. ZXY-2 was originally isolated from Jilin Pesticide Plant (China). Strain ZXY-2 demonstrated excellent atrazine degradation performance and saline tolerance. Here we report the complete genome sequence of strain ZXY-2 contained a circular chromosome and five circular plasmids encoding for the mechanism of salt adaptation and pollutant degradation.
March 14, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28315056/degradation-of-n-alkanes-and-pahs-from-the-heavy-crude-oil-using-salt-tolerant-bacterial-consortia-and-analysis-of-their-catabolic-genes
#9
Ranjit Gurav, Honghong Lyu, Jianli Ma, Jingchun Tang, Qinglong Liu, Hairong Zhang
In the present study, salt-tolerant strains, Dietzia sp. HRJ2, Corynebacterium variabile HRJ4, Dietzia cinnamea HRJ5 and Bacillus tequilensis HRJ6 were isolated from the Dagang oil field, China. These strains degraded n-alkanes and polycyclic aromatic hydrocarbons (PAHs) aerobically from heavy crude oil (HCO) in an experiment at 37 °C and 140 rpm. The GC/MS investigation for degradation of different chain lengths of n-alkanes (C8-C40) by individual strains showed the highest degradation of C8-C19 (HRJ5), C20-C30 (HRJ4) and C31-C40 (HRJ5), respectively...
March 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28303127/functional-characterization-of-endophytic-fungal-community-associated-with-oryza-sativa-l-and-zea-mays-l
#10
Momota Potshangbam, S Indira Devi, Dinabandhu Sahoo, Gary A Strobel
In a natural ecosystem, the plant is in a symbiotic relationship with beneficial endophytes contributing huge impact on its host plant. Therefore, exploring beneficial endophytes and understanding its interaction is a prospective area of research. The present work aims to characterize the fungal endophytic communities associated with healthy maize and rice plants and to study the deterministic factors influencing plant growth and biocontrol properties against phytopathogens, viz, Pythium ultimum, Sclerotium oryzae, Rhizoctonia solani, and Pyricularia oryzae...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28300477/evaluation-of-intracameral-pentablock-copolymer-thermosensitive-gel-for-sustained-drug-delivery-to-the-anterior-chamber-of-the-eye
#11
Elizabeth Schaefer, Sara M Smith, Jacklyn Salmon, Santhi Abbaraju, Rasidul Amin, Sidney Weiss, Ulrich Grau, Poonam Velagaleti, Brian Gilger
PURPOSE: To investigate PTSgels (Pentablock copolymers) as an injectable formulation technology for sustained ocular drug delivery. Drug release profile, tolerability, and polymer degradation for one of the thermosensitive, biodegradable, and biocompatible compositions were investigated through intracameral (IC) injection in rabbits. METHODS: New Zealand White rabbit eyes were injected IC (50 μL) with 100 μg near-infrared-immunoglobulin G (NIR-IgG) in balanced salt solution (BSS) or 20% PTSgel; or with PTSgel or BSS alone...
March 16, 2017: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28300216/ein3-and-sos2-synergistically-modulate-plant-salt-tolerance
#12
Ruidang Quan, Juan Wang, Dexin Yang, Haiwen Zhang, Zhijin Zhang, Rongfeng Huang
Ethylene biosynthesis and the ethylene signaling pathway regulate plant salt tolerance by activating the expression of downstream target genes such as those related to ROS and Na(+)/K(+) homeostasis. The Salt Overly Sensitive (SOS) pathway regulates Na(+)/K(+) homeostasis in Arabidopsis under salt stress. However, the connection between these two pathways is unclear. Through genetic screening, we identified two sos2 alleles as salt sensitive mutants in the ein3-1 background. Neither Ethylene-Insensitive 2 (EIN2) nor EIN3 changed the expression patterns of SOS genes including SOS1, SOS2, SOS3 and SOS3-like Calcium Binding Protein 8 (SCaBP8), but SOS2 activated the expression of one target gene of EIN3, Ethylene and Salt-inducible ERF 1 (ESE1)...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28298921/exogenous-melatonin-confers-salt-stress-tolerance-to-watermelon-by-improving-photosynthesis-and-redox-homeostasis
#13
Hao Li, Jingjing Chang, Hejie Chen, Zhongyuan Wang, Xiurong Gu, Chunhua Wei, Yong Zhang, Jianxiang Ma, Jianqiang Yang, Xian Zhang
Melatonin, a pleiotropic signal molecule, has been shown to play important roles in the regulation of plant growth, development, and responses to environmental stresses. Since a few species have been investigated to unveil the effect of exogenous melatonin on salt stress, the underlying mechanism of melatonin-mediated salt stress tolerance in other plant species still remains largely unknown. In this study, the effects of melatonin on leaf photosynthesis and redox homeostasis in watermelon were examined under salt stress (300 mM NaCl) along with different doses of melatonin (50, 150, and 500 μM) pretreatment...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28298677/uvra-expression-of-lactococcus-lactis-nz9000-improve-multiple-stresses-tolerance-and-fermentation-of-lactic-acid-against-salt-stress
#14
Taher Khakpour Moghaddam, Juan Zhang, Guocheng Du
Lactococcus lactis is subjected to several stressful conditions during industrial fermentation including oxidation, heating and cooling, acid, high osmolarity/dehydration and starvation. DNA lesion is a major cause of genetic instability in L. lactis that usually occurs at a low frequency, but it is greatly enhanced by environmental stresses. DNA damages produced by these environmental stresses are thought to induce DNA double-strand breaks, leading to illegitimate recombination. Nucleotide excision repair (NER) protein UvrA suppresses multiple stresses-induced illegitimate recombination...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28293865/the-inhibition-effect-of-lactobacilli-against-growth-and-biofilm-formation-of-pseudomonas-aeruginosa
#15
Dariush Shokri, Mohammad Rabbani Khorasgani, Milad Mohkam, Seyed Masih Fatemi, Younes Ghasemi, Asghar Taheri-Kafrani
The emergence of antibiotic-resistant and food-spoilage microorganisms has renewed efforts to identify safe and natural alternative agents of antibiotics such as probiotics. The aim of this study was the isolation of lactobacilli as potential probiotics from local dairy products with broad antibacterial and anti-biofilm activities against antibiotic-resistant strains of Pseudomonas aeruginosa and determination of their inhibition mechanism. Antibiotic susceptibility and classification of acquired resistance profiles of 80 P...
March 14, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28293246/brachypodium-distachyon-bdpp2ca6-interacts-with-bdpyls-and-bdsnrk2-and-positively-regulates-salt-tolerance-in-transgenic-arabidopsis
#16
Fan Zhang, Qiuhui Wei, Jiaochun Shi, Xia Jin, Yuan He, Yang Zhang, Qingchen Luo, Yuesheng Wang, Junli Chang, Guangxiao Yang, Guangyuan He
The phytohormone abscisic acid (ABA) is essential in plant responding to biotic and abiotic stresses. Although ABA signaling model is well established in Arabidopsis, ABA receptor PYL family and clade A PP2C subfamily are not yet characterized in monocot model plant Brachypodium distachyon. In this study, we identified 12 PYLs and 8 clade A PP2Cs from B. distachyon genome and successfully cloned 12 PYLs and 7 clade A PP2Cs. Bioinformatic and expression analyses showed that most of the identified genes respond to several signal molecules and abiotic stresses...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28291380/beneficial-rhizobacterium-bacillus-amyloliquefaciens-sqr9-induces-plant-salt-tolerance-through-spermidine-production
#17
Lin Chen, Yunpeng Liu, Gengwei Wu, Nan Zhang, Qi-Rong Shen, Ruifu Zhang
The inoculation of plants with plant growth-promoting rhizobacterium (PGPR) has been an effective strategy for enhancing plant salt tolerance to diminish the loss of agricultural productivity caused by salt stress, but the signal transmitted from bacteria to the plant under salt stress is poorly understood. In this study, the salt tolerance of Arabidopsis thaliana and Zea mays was enhanced by inoculation with Bacillus amyloliquefaciens SQR9. Using dialysis bags with different molecular weight cutoffs, we sorted through the molecules secreted by SQR9 and found that spermidine is responsible for enhancing plant salt tolerance...
March 14, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28290079/silicon-alleviates-salt-and-drought-stress-of-glycyrrhiza-uralensis-seedling-by-altering-antioxidant-metabolism-and-osmotic-adjustment
#18
Wenjin Zhang, Zhicai Xie, Lianhong Wang, Ming Li, Duoyong Lang, Xinhui Zhang
This study was conducted to determine effect and mechanism of exogenous silicon (Si) on salt and drought tolerance of Glycyrrhiza uralensis seedling by focusing on the pathways of antioxidant defense and osmotic adjustment. Seedling growth, lipid peroxidation, antioxidant metabolism, osmolytes concentration and Si content of G. uralensis seedlings were analyzed under control, salt and drought stress [100 mM NaCl with 0, 10 and 20% of PEG-6000 (Polyethylene glycol-6000)] with or without 1 mM Si. Si addition markedly affected the G...
March 13, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/28286906/selection-of-cholesterol-lowering-lactic-acid-bacteria-and-its-effects-on-rats-fed-with-high-cholesterol-diet
#19
Yufang Liu, Fengchun Zhao, Jiye Liu, Huimin Wang, Xiao Han, Yongxin Zhang, Zhengyou Yang
High cholesterol level in serum is a major factor of influence for coronary heart disease. The cholesterol-lowering ability of lactic acid bacteria (LAB) without side effects makes them more and more attractive. Seventy-nine strains of LAB isolated from fermented food were screened in vitro for their ability to assimilate cholesterol. Then, ten strains which exhibited higher ability of cholesterol assimilation were investigated with the characteristics of acidic resistance, bile salt tolerance, and cell adhesion...
March 13, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28285084/a-salt-stress-regulator-from-the-poplar-r2r3-myb-family-integrates-the-regulation-of-lateral-root-emergence-and-aba-signaling-to-mediate-salt-stress-tolerance-in-arabidopsis
#20
Qing Fang, Tianzhi Jiang, Liangxiang Xu, Hai Liu, Hui Mao, Xianqiang Wang, Bo Jiao, Yanjiao Duan, Qiong Wang, Qiannan Dong, Li Yang, Guozheng Tian, Chi Zhang, Yifeng Zhou, Xiaopeng Liu, Haiyang Wang, Di Fan, Bangjun Wang, Keming Luo
The roles of most MYB transcription factors (TFs) in the poplar remain unclear. Here, we demonstrate that PtrSSR1, a salt-stress-regulator in the Populus trichocarpa R2R3 MYB gene family, mediates the tolerance of transgenic Arabidopsis plants to salt stress. The transcripts of PtrSSR1 could be induced by salt stress rapidly in poplar. Subcellular localization and yeast assays indicated that PtrSSR1 encoded a nuclear protein with transactivation activity. The Arabidopsis transformants overexpressing PtrSSR1 clearly displayed lateral root emergence (LRE) inhibition compared with wild-type (Wt) under normal conditions; while upon NaCl treatment, the transformants showed improved tolerance, and the LRs emerged faster from salt-induced inhibition...
February 24, 2017: Plant Physiology and Biochemistry: PPB
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