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https://www.readbyqxmd.com/read/28916739/a-moso-bamboo-wrky-gene-pewrky83-confers-salinity-tolerance-in-transgenic-arabidopsis-plants
#1
Min Wu, Huanlong Liu, Guomin Han, Ronghao Cai, Feng Pan, Yan Xiang
The WRKY family are transcription factors, involved in plant development, and response to biotic and abiotic stresses. Moso bamboo is an important bamboo that has high ecological, economic and cultural value and is widely distributed in the south of China. In this study, we performed a genome-wide identification of WRKY members in moso bamboo and identified 89 members. By comparative analysis in six grass genomes, we found the WRKY gene family may have experienced or be experiencing purifying selection. Based on relative expression levels among WRKY IIc members under three abiotic stresses, PeWRKY83 functioned as a transcription factor and was selected for detailed analysis...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28913795/overview-of-methods-for-assessing-salinity-and-drought-tolerance-of-transgenic-wheat-lines
#2
Rohit Joshi, Khalid Anwar, Priyanka Das, Sneh L Singla-Pareek, Ashwani Pareek
Salinity and drought are interconnected, causing phenotypic, physiological, biochemical, and molecular changes in a cell. These stresses are the major factors adversely affecting growth and productivity in cereals. Genetic engineering methods have advanced to enable development of genotypes with improved salinity and drought tolerance. The resulting transgenic plant produces a group of progenies which includes moderate to high-stress tolerant transgenic lines. Development of reproducible screening methods to identify high-stress tolerant germplasm under laboratory, greenhouse, or field conditions is must...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28911682/relation-between-salt-tolerance-and-biochemical-changes-in-cumin-cuminum-cyminum%C3%A2-l-seeds
#3
Iness Bettaieb Rebey, Soumaya Bourgou, Fatma Zohra Rahali, Kamel Msaada, Riadh Ksouri, Brahim Marzouk
In this study, the effects of salinity on growth, fatty acid, essential oil, and phenolic composition of cumin (Cuminum cyminum L.) seeds as well as the antioxidant activities of their extracts were investigated. Plants were treated with different concentrations of NaCl treatment: 0, 50, 75, and 125 mmoL. Plant growth was significantly reduced with the severity of saline treatment. This also caused important reductions in the seed yield and yield components. Besides, NaCl treatments affected fatty acid composition...
April 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28910580/differential-responses-of-cell-wall-bound-phenolic-compounds-in-sensitive-and-tolerant-varieties-of-rice-in-response-to-salinity
#4
Poulami Gupta, Bratati De
In plants, cell wall bound phenolics change in response to stress. The aim of the study was to investigate the effect of NaCl induced stress on wall bound phenolics in four rice varieties, of which two (Bhutnath, Nonabokra) were salt tolerant and two (MTU 7029, Sujala) were salt sensitive. After germination, seedlings were grown in hydroponic solution and subjected to salinity stress (25mM, 50mM, 100mM and 150mM NaCl) on day 12. Wall bound phenolic compounds were determined by GC-MS based metabolite analysis...
September 14, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28905285/effect-of-bacteriocin-and-exopolysaccharides-isolated-from-probiotic-on-p-aeruginosa-pao1-biofilm
#5
Vivek Sharma, Kusum Harjai, Geeta Shukla
Microorganisms develop biofilms on indwelling medical devices and are associated with biofilm-related infections, resulting in substantial morbidity and mortality. Therefore, to prevent and control biofilm-associated infections, the present study was designed to assess the anti-biofilm potential of postbiotics derived from probiotic organisms against most prevalent biofilm-forming Pseudomonas aeruginosa PAO1. Eighty lactic acid bacteria isolated from eight neonatal fecal samples possessed antibacterial activity against P...
September 14, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28904958/transcriptomic-analysis-reveals-genes-mediating-salt-tolerance-through-calcineurin-ccha-independent-signaling-in-aspergillus-nidulans
#6
Sha Wang, Hongchang Zhou, Jun Wu, Jiangyu Han, Shasha Li, Shengwen Shao
Adaptation to changes in the environment is crucial for the viability of all organisms. Although the importance of calcineurin in the stress response has been highlighted in filamentous fungi, little is known about the involvement of ion-responsive genes and pathways in conferring salt tolerance without calcium signaling. In this study, high-throughput RNA-seq was used to investigate salt stress-induced genes in the parent, ΔcnaB, and ΔcnaBΔcchA strains of Aspergillus nidulans, which differ greatly in salt adaption...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28904348/transcriptome-profiling-of-genes-involved-in-induced-systemic-salt-tolerance-conferred-by-bacillus-amyloliquefaciens-fzb42-in-arabidopsis-thaliana
#7
Shaofang Liu, Haiting Hao, Xiang Lu, Xia Zhao, Yun Wang, Yubao Zhang, Zhongkui Xie, Ruoyu Wang
Plant growth-promoting Bacillus amyloliquefaciens FZB42 induces systemic salt tolerance in Arabidopsis and enhances the fresh and dry weight. However, the underlying molecular mechanism that allows plants to respond to FZB42 and exhibit salt tolerance is largely unknown. Therefore, we performed large-scale transcriptome sequencing of Arabidopsis shoot tissues grown under salt stress with or without FZB42 inoculation by using Illumina sequencing to identify the key genes and pathways with important roles during this interaction...
September 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28903734/transcriptomic-profiling-of-genes-in-matured-dimorphic-seeds-of-euhalophyte-suaeda-salsa
#8
Yange Xu, Yuanqin Zhao, Huimin Duan, Na Sui, Fang Yuan, Jie Song
BACKGROUND: Suaeda salsa (S. salsa) is a euhalophyte with high economic value. S. salsa can produce dimorphic seeds. Brown seeds are more salt tolerant, can germinate quickly and maintain the fitness of the species under high saline conditions. Black seeds are less salt tolerant, may become part of the seed bank and germinate when soil salinity is reduced. Previous reports have mainly focused on the ecophysiological traits of seed germination and production under saline conditions in this species...
September 13, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28902865/optimization-of-the-expression-purification-and-polymerase-activity-reaction-conditions-of-recombinant-human-primpol
#9
Elizaveta O Boldinova, Gorazd Stojkovič, Rafil Khairullin, Sjoerd Wanrooij, Alena V Makarova
Human PrimPol is a DNA primase/polymerase involved in DNA damage tolerance and prevents nuclear genome instability. PrimPol is also localized to the mitochondria, but its precise function in mitochondrial DNA maintenance has remained elusive. PrimPol works both as a translesion (TLS) polymerase and as the primase that restarts DNA replication after a lesion. However, the observed biochemical activities of PrimPol vary considerably between studies as a result of different reaction conditions used. To reveal the effects of reaction composition on PrimPol DNA polymerase activity, we tested the polymerase activity in the presence of various buffer agents, salt concentrations, pH values and metal cofactors...
2017: PloS One
https://www.readbyqxmd.com/read/28900441/bacillus-amyloliquefaciens-confers-tolerance-to-various-abiotic-stresses-and-modulates-plant-response-to-phytohormones-through-osmoprotection-and-gene-expression-regulation-in-rice
#10
Shalini Tiwari, Vivek Prasad, Puneet S Chauhan, Charu Lata
Being sessile in nature, plants have to withstand various adverse environmental stress conditions including both biotic and abiotic stresses. Comparatively, abiotic stresses such as drought, salinity, high temperature, and cold pose major threat to agriculture by negatively impacting plant growth and yield worldwide. Rice is one of the most widely consumed staple cereals across the globe, the production and productivity of which is also severely affected by different abiotic stresses. Therefore, several crop improvement programs are directed toward developing stress tolerant rice cultivars either through marker assisted breeding or transgenic technology...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28900229/a-class-ii-small-heat-shock-protein-oshsp18-0-plays-positive-roles-in-both-biotic-and-abiotic-defense-responses-in-rice
#11
Jie Kuang, Jianzhong Liu, Jun Mei, Changchun Wang, Haitao Hu, Yanjun Zhang, Meihao Sun, Xi Ning, Langtao Xiao, Ling Yang
Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most devastating diseases of rice. However, the molecular mechanism underpinning the Xoo resistance of rice is still not fully understood. Here, we report that a class II small heat shock protein gene, OsHsp18.0, whose expression was differentially induced between a resistant and a susceptible variety in response to Xoo infection, plays positive roles in both biotic and abiotic resistance. The molecular chaperone activity of OsHsp18...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28900209/iron-oxide-nanoparticles-can-cross-plasma-membranes
#12
Daniele Zanella, Elena Bossi, Rosalba Gornati, Carlos Bastos, Nuno Faria, Giovanni Bernardini
Iron deficiency is a major global public health problem despite decades of efforts with iron supplementation and fortification. The issue lies on the poor tolerability of the standard of care soluble iron salts, leading to non-compliance and ineffective correction of iron-deficiency anaemia. Iron nanoformulations have been proposed to fortify food and feed to address these issues. Since it was just postulated that some nanoparticles (NPs) might cross the plasma membrane also by a non-endocytotic pathway gaining direct access to the cytoplasm, we have studied iron NP uptake under this perspective...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28899514/citral-a-monoterpenoid-aldehyde-interacts-synergistically-with-norfloxacin-against-methicillin-resistant-staphylococcus-aureus
#13
Priyanka Gupta, Dinesh Kumar Patel, Vivek Kumar Gupta, Anirban Pal, Sudeep Tandon, M P Darokar
BACKGROUND: Staphylococcus aureus (SA), is a major human pathogen causing wide range of clinical infections, which has been further complicated by drug resistance like methicillin resistant S. aureus (MRSA), vancomycin intermediate S. aureus (VISA)/vancomycin resistant S. aureus (VRSA), etc. The present study was aimed at determining anti-staphylococcal potential of citral against drug resistant clinical isolates alone and in combination with antibiotics. PURPOSE: To assess the potential of citral in combination with norfloxacin in treating drug resistant infections of SA...
October 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28898746/plant-growth-promoting-endophyte-piriformospora-indica-alleviates-salinity-stress-in-medicago-truncatula
#14
Liang Li, Lei Li, Xiaoyang Wang, Pengyue Zhu, Hongqing Wu, Shuting Qi
Piriformospora indica, a cultivable root endophytic fungus, induces growth promotion as well as biotic stress resistance and tolerance to abiotic stress in a broad range of host plants. In this study, the potential protection for M Medicago truncatula plants from salinity stress by P. indica was explored. The improved plant growth under severe saline condition was exhibited in P. indica-colonized lines. Moreover, the antioxidant enzymes activities and hyphae density in roots were increased by the endophyte under high salt concentration...
September 1, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28895882/physiological-and-transcriptomic-responses-of-chinese-cabbage-brassica-rapa-l-ssp-pekinensis-to-salt-stress
#15
Nianwei Qiu, Qian Liu, Jingjuan Li, Yihui Zhang, Fengde Wang, Jianwei Gao
Salt stress is one of the major abiotic stresses that severely impact plant growth and development. In this study, we investigated the physiological and transcriptomic responses of Chinese cabbage "Qingmaye" to salt stress, a main variety in North China. Our results showed that the growth and photosynthesis of Chinese cabbage were significantly inhibited by salt treatment. However, as a glycophyte, Chinese cabbage could cope with high salinity; it could complete an entire life cycle at 100 mM NaCl. The high salt tolerance of Chinese cabbage was achieved by accumulating osmoprotectants and by maintaining higher activity of antioxidant enzymes...
September 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28895014/the-responses-of-two-native-plant-species-to-soil-petroleum-contamination-in-the-yellow-river-delta-china
#16
Wenjun Xie, Yanpeng Zhang, Rui Li, Hongjun Yang, Tao Wu, Liping Zhao, Zhaohua Lu
Petroleum contamination is a significant environmental problem in the Yellow River Delta. The responses of two native salt-tolerant plant species, alfalfa (Medicago sativa) and bristle grass (Setaria uiridis Beauv), to soil petroleum contamination were investigated at five levels between 0 and 2.0% (w/w). Results showed that the total, aboveground and underground plant biomasses of both species were significantly reduced by petroleum contamination (p < 0.05), with the inhibition enhanced with increased petroleum levels...
September 11, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28894951/structural-and-functional-characterization-of-mercuric-reductase-from-lysinibacillus-sphaericus-strain-g1
#17
Amit Bafana, Farha Khan, Kaza Suguna
In response to the widespread presence of inorganic Hg in the environment, bacteria have evolved resistance systems with mercuric reductase (MerA) as the key enzyme. MerA enzymes have still not been well characterized from gram positive bacteria. Current study reports physico-chemical, kinetic and structural characterization of MerA from a multiple heavy metal resistant strain of Lysinibacillus sphaericus, and discusses its implications in bioremediation application. The enzyme was homodimeric with subunit molecular weight of about 60 kDa...
September 11, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/28893666/physiological-and-behavioral-responses-to-salinity-in-coastal-dice-snakes
#18
François Brischoux, Yurii V Kornilev, Harvey B Lillywhite
Secondarily marine tetrapods have evolved adaptations to maintain their osmotic balance in a hyperosmotic environment. During the transition to a marine habitat, the evolution of a euryhaline physiology likely encompassed successive changes in behavior and physiology that released organisms from regular access to fresh water. Deciphering these key steps is a complicated task. In this study, we investigated a species of freshwater natricine snake in which some populations are known to use marine environments...
September 8, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28893659/a-halotolerant-enterobacter-sp-displaying-acc-deaminase-activity-promotes-rice-seedling-growth-under-salt-stress
#19
Anumita Sarkar, Pallab Kumar Ghosh, Krishnendu Pramanik, Soumik Mitra, Tithi Soren, Sanjeev Pandey, Monohar Hossain Mondal, Tushar Kanti Maiti
Agricultural productivity is proven to be hampered by the synthesis of reactive oxygen species (ROS) and production of stress-induced ethylene under salinity stress. One-aminocyclopropane-1-carboxylic acid (ACC) is the direct precursor of ethylene synthesized by plants. Bacteria possessing ACC deaminase activity can use ACC as a nitrogen source preventing ethylene production. Several salt-tolerant bacterial strains displaying ACC deaminase activity were isolated from rice fields, and their plant growth-promoting (PGP) properties were determined...
September 9, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28888960/complete-genome-sequence-of-endophyte-bacillus-flexus-klbmp-4941-reveals-its-plant-growth-promotion-mechanism-and-genetic-basis-for-salt-tolerance
#20
Tian-Tian Wang, Peng Ding, Pan Chen, Ke Xing, Juan-Luan Bai, Wen Wan, Ji-Hong Jiang, Sheng Qin
Bacillus flexus KLBMP 4941 is a halotolerant endophyte isolated from the halophyte Limonium sinense. This strain can improve host seedling growth under salt stress conditions. We here report the complete genome information of endophyte KLBMP 4941. It has a circular chromosome and two plasmids for a total genome 4,104,242 bp in size with a G+C content of 38.09%. Genes related to plant growth promotion (PGP), such as those associated with nitrogen fixation, siderophore, spermidine, and acetoin synthesis were found in the KLBMP 4941 genome...
September 6, 2017: Journal of Biotechnology
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