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https://www.readbyqxmd.com/read/28213032/applicability-of-a-colorimetric-method-for-evaluation-of-lactic-acid-bacteria-with-probiotic-properties
#1
Min Young Jung, Jina Lee, Boyeon Park, Hyelyeon Hwang, Sung-Oh Sohn, Se Hee Lee, Hyeong In Lim, Hae Woong Park, Jong-Hee Lee
Rapid colorimetric methods using various indicator reagents have been developed to monitor bacterial viability. Here, we examined the applicability of a method based on the reduction of resazurin or water-soluble tetrazolium salt-8 (WST-8) to screen lactic acid bacteria (LAB) for growth, tolerance against bile acid and low pH. The resazurin reduction test proved unsuitable for screening LAB such as Lactobacillus plantarum and Leuconostoc mesenteroides since it reacted with acid present in the cultures. LAB growth could be indirectly quantified by measuring WST-8 reduction...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28208265/effect-of-nanoemitters-on-suppressing-the-formation-of-metal-adduct-ions-in-electrospray-ionization-mass-spectrometry
#2
Jun Hu, Qi-Yuan Guan, Jiang Wang, Xiao-Xiao Jiang, Zeng-Qiang Wu, Xing-Hua Xia, Jing-Juan Xu, Hong-Yuan Chen
In the work, we showed that the use of nanoemitters (tip dimension <1 μm, typically ∼100 nm) could dramatically reduce the nonspecific metal adduction to peptide or protein ions as well as improve the matrix tolerance of electrospray ionization mass spectrometry (ESI-MS). The proton-enriched smaller initial droplets are supposed to have played a significant role in suppressing the formation of metal adduct ions in nanoemitters. The proton-enrichment effect in the nanoemitters is related to both the exclusion-enrichment effect (EEE) and the ion concentration polarization effect (ICP effect), which permit the molecular ions to be regulated to protonated ones...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28203228/encapsulation-of-antifungals-in-micelles-protects-candida-albicans-during-gall-bladder-infection
#3
Shih-Hung Hsieh, Sascha Brunke, Matthias Brock
Candida albicans is a dimorphic fungus that colonizes human mucosal surfaces with the potential to cause life-threatening invasive candidiasis. Studies on systemic candidiasis in a murine infection model using in vivo real-time bioluminescence imaging revealed persistence of C. albicans in the gall bladder under antifungal therapy. Preliminary analyses showed that bile conferred resistance against a wide variety of antifungals enabling survival in this cryptic host niche. Here, bile and its components were studied for their ability to reduce antifungal efficacy in order to elucidate the underlying mechanism of protection...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28203227/some-technological-properties-of-lactic-acid-bacteria-isolated-from-dahi-and-datshi-naturally-fermented-milk-products-of-bhutan
#4
H N J Shangpliang, Sharmila Sharma, Ranjita Rai, Jyoti P Tamang
Dahi and datshi are common naturally fermented milk (NFM) products of Bhutan. Population of lactic acid bacteria (LAB) in dahi (pH 3.7) and datshi (pH 5.2) was 1.4 × 10(7) and 3.9 × 10(8) cfu/ml, respectively. Based on 16S rRNA gene sequencing isolates of LAB from dahi and datshi were identified as Enterococcus faecalis, E. faecium, Lactococcus lactis subsp. lactis. LAB strains were tested for some technological properties. All LAB strains except E. faecalis CH2:17 caused coagulation of milk at both 30°C for 48 h...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28198821/the-microbe-secreted-isopeptide-poly-%C3%AE-glutamic-acid-induces-stress-tolerance-in-brassica-napus-l-seedlings-by-activating-crosstalk-between-h2o2-and-ca-2
#5
Peng Lei, Xiao Pang, Xiaohai Feng, Sha Li, Bo Chi, Rui Wang, Zongqi Xu, Hong Xu
Poly-γ-glutamic acid (γ-PGA) is a microbe-secreted isopeptide that has been shown to promote growth and enhance stress tolerance in crops. However, its site of action and downstream signaling pathways are still unknown. In this study, we investigated γ-PGA-induced tolerance to salt and cold stresses in Brassica napus L. seedlings. Fluorescent labeling of γ-PGA was used to locate the site of its activity in root protoplasts. The relationship between γ-PGA-induced stress tolerance and two signal molecules, H2O2 and Ca(2+), as well as the γ-PGA-elicited signaling pathway at the whole plant level, were explored...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28192458/transcriptome-analysis-of-hexaploid-hulless-oat-in-response-to-salinity-stress
#6
Bin Wu, Yani Hu, Pengjie Huo, Qian Zhang, Xin Chen, Zongwen Zhang
BACKGROUND: Oat is a cereal crop of global importance used for food, feed, and forage. Understanding salinity stress tolerance mechanisms in plants is an important step towards generating crop varieties that can cope with environmental stresses. To date, little is known about the salt tolerance of oat at the molecular level. To better understand the molecular mechanisms underlying salt tolerance in oat, we investigated the transcriptomes of control and salt-treated oat using RNA-Seq. RESULTS: Using Illumina HiSeq 4000 platform, we generated 72,291,032 and 356,891,432 reads from non-stressed control and salt-stressed oat, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28191739/itraq-based-quantitative-proteomic-analysis-of-wheat-roots-in-response-to-salt-stress
#7
Qiyan Jiang, Xiaojuan Li, Fengjuan Niu, Xianjun Sun, Zheng Hu, Hui Zhang
Salinity is a major abiotic stress that affects plant growth and development. Plant roots are the sites of salt uptake. Here, an isobaric tag for a relative and absolute quantitation (iTRAQ)-based proteomic technique was employed to identify the differentially expressed proteins (DEPs) from seedling roots of the salt-tolerant genotype Han 12 and the salt-sensitive genotype Jimai 19 in response to salt treatment. A total of 121 NaCl-responsive DEPs were observed in Han 12 and Jimai 19. The main DEPs were ubiquitination-related proteins, transcription factors, pathogen-related proteins, membrane intrinsic protein transporters and antioxidant enzymes, which may work together to obtain cellular homeostasis in roots and determine the overall salt tolerance of different wheat varieties in response to salt stress...
February 13, 2017: Proteomics
https://www.readbyqxmd.com/read/28187710/ionomic-and-metabolic-responses-to-neutral-salt-or-alkaline-salt-stresses-in-maize-zea-mays-l-seedlings
#8
Rui Guo, LianXuan Shi, Changrong Yan, Xiuli Zhong, FengXue Gu, Qi Liu, Xu Xia, Haoru Li
BACKGROUND: Soil salinity and alkalinity present a serious threat to global agriculture. However, most of the studies have focused on neutral salt stress, and the information on the metabolic responses of plants to alkaline salt stress is limited. This investigation aimed at determining the influence of neutral salt and alkaline salt stresses on the content of metal elements and metabolites in maize plant tissues, by using mixtures of various proportions of NaCl, NaHCO3, Na2SO4, and Na2CO3...
February 10, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28186764/conformational-and-colloidal-stabilities-of-human-immunoglobulin-g-fc-and-its-cyclized-variant-independent-and-compensatory-participation-of-domains-in-aggregation-of-multi-domain-proteins
#9
Seiki Yageta, Risa Shibuya, Hiroshi Imamura, Shinya Honda
Monoclonal immunoglobulin G (IgG) is a multi-domain protein. It has been reported that the conformational and colloidal stabilities of each domain are different and it is predicted that limited domains participate in IgG aggregation. In contrast, the influence of inter-domain interactions on IgG aggregation remains unclear. The fragment crystallizable (Fc) region is also a multi-domain protein consisting of two sets of CH2 and CH3 domains. Here, we have analyzed the conformational change and aggregate size of an aglycosylated Fc region induced by both acid- and salt-stresses, and have elucidated the influence of inter-domain interactions between CH2 and CH3 domains on the conformational and colloidal stabilities of the aglycosylated Fc region...
February 10, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28184229/bnsip1-1-a-trihelix-family-gene-mediates-abiotic-stress-tolerance-and-aba-signaling-in-brassica-napus
#10
Junling Luo, Shaohua Tang, Fengling Mei, Xiaojue Peng, Jun Li, Xiaofei Li, Xiaohong Yan, Xinhua Zeng, Fang Liu, Yuhua Wu, Gang Wu
The trihelix family genes have important functions in light-relevant and other developmental processes, but their roles in response to adverse environment are largely unclear. In this study, we identified a new gene, BnSIP1-1, which fell in the SIP1 (6b INTERACTING PROTEIN1) clade of the trihelix family with two trihelix DNA binding domains and a fourth amphipathic α-helix. BnSIP1-1 protein specifically targeted to the nucleus, and its expression can be induced by abscisic acid (ABA) and different stresses...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28184228/marker-aided-incorporation-of-saltol-a-major-qtl-associated-with-seedling-stage-salt-tolerance-into-oryza-sativa-pusa-basmati-1121
#11
N Naresh Babu, S Gopala Krishnan, K K Vinod, S L Krishnamurthy, Vivek K Singh, Madan P Singh, Renu Singh, Ranjith K Ellur, Vandna Rai, Haritha Bollinedi, Prolay K Bhowmick, Ashutosh K Yadav, Mariappan Nagarajan, Nagendra K Singh, Kumble V Prabhu, Ashok K Singh
Pusa Basmati 1121 (PB1121), an elite Basmati rice cultivar is vulnerable to salinity at seedling stage. A study was undertaken to impart seedling-stage salt tolerance into PB1121 by transferring a quantitative trait locus (QTL), Saltol, using FL478 as donor, through marker assisted backcrossing. Sequence tagged microsatellite site (STMS) marker RM 3412, tightly linked to Saltol was used for foreground selection. Background recovery was estimated using 90 genome-wide STMS markers. Systematic phenotypic selection helped in accelerated recovery of recurrent parent phenome (RPP)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28179101/molecular-phylogenomic-study-and-the-role-of-exogenous-spermidine-in-the-metabolic-adjustment-of-endogenous-polyamine-in-two-rice-cultivars-under-salt-stress
#12
Jayita Saha, Kalyan Giri
Compelling evidences anticipated the well acclamation of involvement of exogenous and endogenous polyamines (PAs) in conferring salt tolerance in plants. Intracellular PA's anabolism and catabolism should have contributed to maintain endogenous PAs homeostasis to induce stress signal networks. In this report, the evolutionary study has been conducted to reveal the phylogenetic relationship of genes encoding enzymes of the anabolic and catabolic pathway of PAs among the five plant lineages including green algae, moss, lycophyte, dicot and monocot along with their respective exon-intron structural patterns...
February 5, 2017: Gene
https://www.readbyqxmd.com/read/28178573/enhancement-of-cold-and-salt-tolerance-of-arabidopsis-by-transgenic-expression-of-the-s-adenosylmethionine-decarboxylase-gene-from-leymus-chinensis
#13
Zhujiang Liu, Panpan Liu, Dongmei Qi, Xianjun Peng, Gongshe Liu
Leymus chinensis is an important perennial forage grass natively distributed in the Eurasian Steppe. However, little is known about the molecular mechanism of its adaptation to extreme environmental conditions. Based on L. chinensis cold-treated sequence database, a highly expressed S-adenosylmethionine decarboxylase gene (LcSAMDC1) was isolated from L. chinensis. Gene structure analysis showed that LcSAMDC1 has two introns and three exons as well as three non-overlapping ORFs in its mRNA sequence. One hour of cold exposure caused a significant up-regulation of LcSAMDC1, while abscisic acid (ABA), salt, and osmotic stresses slightly induced its expression...
January 19, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28178571/genome-wide-identification-and-analysis-of-biotic-and-abiotic-stress-regulation-of-small-heat-shock-protein-hsp20-family-genes-in-bread-wheat
#14
Senthilkumar K Muthusamy, Monika Dalal, Viswanathan Chinnusamy, Kailash C Bansal
Small Heat Shock Proteins (sHSPs)/HSP20 are molecular chaperones that protect plants by preventing protein aggregation during abiotic stress conditions, especially heat stress. Due to global climate change, high temperature is emerging as a major threat to wheat productivity. Thus, the identification of HSP20 and analysis of HSP transcriptional regulation under different abiotic stresses in wheat would help in understanding the role of these proteins in abiotic stress tolerance. We used sequences of known rice and Arabidopsis HSP20 HMM profiles as queries against publicly available wheat genome and wheat full length cDNA databases (TriFLDB) to identify the respective orthologues from wheat...
January 22, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28177802/plant-growth-promotion-properties-of-bacterial-strains-isolated-from-the-rhizosphere-of-the-jerusalem-artichoke-i-helianthus-tuberosus-i-l-adapted-to-salt-alkaline-soils-and-their-effect-on-wheat-growth
#15
Xiaolin Liu, Xiangyue Li, Yan Li, Runzhi Li, Zhihong Xie
The Jerusalem artichoke (JA; <i>Helianthus tuberosus</i>), known to be tolerant to salt-alkaline soil conditions, has been cultivated for many years in the Yellow River delta, Shandong Province coastal zone, in China. The aim of our study was to isolate nitrogen-fixing bacteria colonizing the rhizosphere of JA and characterize other plant growth promotion properties. The ultimate goal was to identify isolates that could be used as inoculant benefiting an economic crop, in particular for improving wheat growth production in the Yellow River delta...
November 14, 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28177799/the-effects-of-pantoea-sp-y4-4-on-alfalfa-in-the-remediation-of-heavy-metal-contaminated-soil-and-auxiliary-impacts-of-plant-residues-on-the-remediation-of-saline-alkali-soils
#16
Shuhuan Li, Jie Wang, Nanxiong Gao, Lizhu Liu, Yahua Chen
The plant growth-promoting rhizobacteria (PGPR) Y4-4 was isolated from plant rhizosphere soil and identified as Pantoea sp. by 16S rRNA sequence analysis. The effects of strain Y4-4 on alfalfa grew in heavy metals contaminated soil was investigated using a pot experiment. In Cu rich environment, the dry weight from shoot and total of plant inoculated with strain Y4-4 increased by 22.6% and 21%, and Cu accumulation increased by 15%. In Pb-Zn rich environment, the dry weight from shoot and total of plant inoculated with strain Y4-4 increased by 23...
November 30, 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28169318/effect-of-exogenous-selenium-supply-on-photosynthesis-na-accumulation-and-antioxidative-capacity-of-maize-zea-mays-l-under-salinity-stress
#17
Chaoqiang Jiang, Chaolong Zu, Dianjun Lu, Qingsong Zheng, Jia Shen, Huoyan Wang, Decheng Li
The mechanism of selenium-mediated salt tolerance has not been fully clarified. This study investigated the possible role of selenium (Se) in regulating maize salt tolerance. A pot experiment was conducted to investigate the role of Se (0, 1, 5 and 25 μM Na2SeO3) in photosynthesis, antioxidative capacity and ion homeostasis in maize under salinity. The results showed that Se (1 μM) relieved the salt-induced inhibitory effects on the plant growth and development of 15-day-old maize plants. Se application (1 μM) also increased the net photosynthetic rate and alleviated the damage to chloroplast ultrastructure induced by NaCl...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28169290/knockdown-of-a-cellulose-synthase-gene-boicesa-affects-the-leaf-anatomy-cellulose-content-and-salt-tolerance-in-broccoli
#18
Shuangtao Li, Lei Zhang, Ying Wang, Fengfeng Xu, Mengyun Liu, Peng Lin, Shuxin Ren, Rui Ma, Yang-Dong Guo
Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse orientations to construct the recombinant RNAi vector. Knockdown of BoiCesA caused "dwarf" phenotype with smaller leaves and a loss of the content of cellulose. Moreover, RT-PCR analysis confirmed that the expression of the RNAi apparatus could repress expression of the CesA gene. Meanwhile, examination of the leaves from the T3 of RNAi transformants indicated reduction of cell expansion in vascular bundles, particularly on their abaxial surface...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28167028/salinity-induced-accumulation-of-endogenous-h2s-and-no-is-associated-with-modulation-of-the-antioxidant-and-redox-defense-systems-in-nicotiana-tabacum-l-cv-havana
#19
Cristiane Jovelina da Silva, Elizabeth Pacheco Batista Fontes, Luzia Valentina Modolo
Salinity is one of the abiotic factors that most affect crop growth and production. This study focused on the effect of high salinity on the endogenous levels of the signaling molecules hydrogen sulfite (H2S) and nitric oxide (NO) in Nicotiana tabacum leaves and the extent of these for the biochemically-driven plant tolerance to such abiotic stress. The NaCl treatment for 10days led to an expressive augment of H2S and NO levels. This increase was correlated with the raise of l-Cys and l-Arg and the induction of l-cysteine desulfhydrase, cyanoalanine synthase, cysteine synthase, nitrate reductase and arginase, enzymes known to be involved in the biosynthesis of H2S or NO...
March 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28163824/draft-genome-sequence-of-lactobacillus-plantarum-strains-e2c2-and-e2c5-isolated-from-human-stool-culture
#20
Mangesh V Suryavanshi, Dhiraj Paul, Swapnil P Doijad, Shrikant S Bhute, Tejashri B Hingamire, Rahul P Gune, Yogesh S Shouche
Probiotic Lactobacillus species offer various health benefits, thus have been employed in treatment and prevention of various diseases. Due to the differences in the isolation source and the site of action, most of the lactobacilli tested in-vitro for probiotics properties fail to extend similar effects in-vivo. Consequently, the search of autochthonous, efficacious and probably population specific probiotics is a high priority in the probiotics research. In this regards, whole genome sequencing of as many Lactobacillus as possible will help to deepen our understanding of biology and their health effects...
2017: Standards in Genomic Sciences
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