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https://www.readbyqxmd.com/read/27920624/neoadjuvant-chemotherapy-with-docetaxel-carboplatin-and-weekly-trastuzumab-is-active-in-her2-positive-early-breast-cancer-results-after-a-median-follow-up-of-over-4-years
#1
Hans-Christian Kolberg, Leyla Akpolat-Basci, Miltiades Stephanou, Bahriye Aktas, Carla Verena Hannig, Cornelia Liedtke
INTRODUCTION: Most patients with HER2-positive breast cancer receive chemotherapy and trastuzumab. Data from adjuvant trials have shown that the combination of docetaxel, carboplatin and weekly trastuzumab (TCH) is well tolerated and as effective as anthracycline-containing regimes. Previous investigations on neoadjuvant treatment with taxanes, platinum salts and trastuzumab showed pathological complete remission (pCR) rates between 43.3 and 76%. To date, the longest published follow-up in this indication is 3 years...
October 2016: Breast Care
https://www.readbyqxmd.com/read/27919704/global-transcriptome-analysis-of-halolamina-sp-to-decipher-the-salt-tolerance-in-extremely-halophilic-archaea
#2
Aslıhan Kurt-Kızıldoğan, Büşra Abanoz, Sezer Okay
Extremely halophilic archaea survive in the hypersaline environments such as salt lakes or salt mines. Therefore, these microorganisms are good sources to investigate the molecular mechanisms underlying the tolerance to high salt concentrations. In this study, a global transcriptome analysis was conducted in an extremely halophilic archaeon, Halolamina sp. YKT1, isolated from a salt mine in Turkey. A comparative RNA-seq analysis was performed using YKT1 isolate grown either at 2.7M NaCl or 5.5M NaCl concentrations...
December 2, 2016: Gene
https://www.readbyqxmd.com/read/27917349/effect-of-acute-salinity-stress-on-ion-homeostasis-na-k-atpase-and-histological-structure-in-sea-cucumber-apostichopus-japonicus
#3
Chenfan Geng, Yi Tian, Yanpeng Shang, Liqiang Wang, Yanan Jiang, Yaqing Chang
BACKGROUND: Sea cucumbers (Apostichopus japonicus) are an imperiled fauna exposed to a variety of environmental condition such as salinity and studies are urgently needed to assess their effects to guide aquaculture efforts. The effects of acute salinity stress on coelomic fluid osmotic pressure, ion concentrations, the activity of Na(+)/K(+)-ATPase in respiratory trees and the histological variations were measured to evaluate the salinity tolerance of sea cucumbers. RESULTS: Significant correlations in osmotic pressure were observed between coelomic fluid and ambient environmental salinity...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27909744/in-vitro-and-in-vivo-evaluation-of-the-probiotic-attributes-of-lactobacillus-kefiranofaciens-xl10-isolated-from-tibetan-kefir-grain
#4
Zhuqing Xing, Wei Tang, Weitao Geng, Yongna Zheng, Yanping Wang
Lactobacillus kefiranofaciens XL10, with a high yield of extracellular polysaccharide (EPS), is isolated from Tibetan kefir grain and benefits the health of human beings and has been considered to exhibit probiotic potential in vitro and in vivo. The probiotic function of the strain was studied extensively, viz., acid and bile salt tolerances, cell surface hydrophobicity and autoaggregation, the modulation of gut microbiota, and the distribution and colonization of XL10 in the mouse intestinal tract after oral administration...
December 1, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27909441/apple-f-box-protein-mdmax2-regulates-plant-photomorphogenesis-and-stress-response
#5
Jian-Ping An, Rui Li, Feng-Jia Qu, Chun-Xiang You, Xiao-Fei Wang, Yu-Jin Hao
MAX2 (MORE AXILLARY GROWTH2) is involved in diverse physiological processes, including photomorphogenesis, the abiotic stress response, as well as karrikin and strigolactone signaling-mediated shoot branching. In this study, MdMAX2, an F-box protein that is a homolog of Arabidopsis MAX2, was identified and characterized. Overexpression of MdMAX2 in apple calli enhanced the accumulation of anthocyanin. Ectopic expression of MdMAX2 in Arabidopsis exhibited photomorphogenesis phenotypes, including increased anthocyanin content and decreased hypocotyl length...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27909432/volatile-mediated-effects-predominate-in-paraburkholderia-phytofirmans-growth-promotion-and-salt-stress-tolerance-of-arabidopsis-thaliana
#6
Thomas Ledger, Sandy Rojas, Tania Timmermann, Ignacio Pinedo, María J Poupin, Tatiana Garrido, Pablo Richter, Javier Tamayo, Raúl Donoso
Abiotic stress has a growing impact on plant growth and agricultural activity worldwide. Specific plant growth promoting rhizobacteria have been reported to stimulate growth and tolerance to abiotic stress in plants, and molecular mechanisms like phytohormone synthesis and 1-aminocyclopropane-1-carboxylate deamination are usual candidates proposed to mediate these bacterial effects. Paraburkholderia phytofirmans PsJN is able to promote growth of several plant hosts, and improve their tolerance to chilling, drought and salinity...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27904462/overexpression-of-a-pathogenesis-related-protein-10-enhances-biotic-and-abiotic-stress-tolerance-in-rice
#7
Jingni Wu, Sang Gon Kim, Kyu Young Kang, Ju-Gon Kim, Sang-Ryeol Park, Ravi Gupta, Yong Hwan Kim, Yiming Wang, Sun Tae Kim
Pathogenesis-related proteins play multiple roles in plant development and biotic and abiotic stress tolerance. Here, we characterize a rice defense related gene named "jasmonic acid inducible pathogenesis-related class 10" (JIOsPR10) to gain an insight into its functional properties. Semi-quantitative RT-PCR analysis showed up-regulation of JIOsPR10 under salt and drought stress conditions. Constitutive over-expression JIOsPR10 in rice promoted shoot and root development in transgenic plants, however, their productivity was unaltered...
December 2016: Plant Pathology Journal
https://www.readbyqxmd.com/read/27902279/desulfonatronospira-sulfatiphila-sp-nov-and-desulfitispora-elongata-sp-nov-the-two-novel-haloalkaliphilic-sulfidogenic-bacteria-from-soda-lakes
#8
Dimitry Y Sorokin, Nikolai A Chernyh
Two novel haloalkaliphilic bacteria with dissimilatory sulfidogenic metabolism were recovered from syntrophic associations obtained from anaerobic sediments of hypersaline soda lakes in Kulunda Steppe (Altai, Russia). Strain ASO3-2T was a member of a sulfidogenic syntrophic association oxidizing acetate at extremely haloalkaline conditions, and was isolated in pure culture using formate as electron donor and sulfate as electron acceptor. It was identified as a new member of the genus Desulfonatronospira within the Deltaproteobacteria...
November 8, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902199/marinomonas-blandensis-sp-nov-a-novel-marine-gammaproteobacterium
#9
David R Arahal, Teresa Lucena, M Carmen Macián, María A Ruvira, José M González, Itziar Lekumberri, Jarone Pinhassi, María J Pujalte
A novel Gram-staining-negative, chemoorganotrophic, moderately halophilic, strictly aerobic bacterium, strain MED121T, was isolated from a seawater sample collected at the Blanes Bay Microbial Observatory in the north-western Mediterranean Sea. Analysis of its 16S rRNA gene sequence, retrieved from the whole-genome sequence, showed that this bacterium was most closely related to Marinomonas dokdonensis, and other Marinomonas spp. (96.3 % and 93.3-95.7 % sequence similarities, respectively), within the family Oceanospirillaceae...
October 9, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27900724/suppression-of-osvpe3-enhances-salt-tolerance-by-attenuating-vacuole-rupture-during-programmed-cell-death-and-affects-stomata-development-in-rice
#10
Wenyun Lu, Minjuan Deng, Fu Guo, Mingqiang Wang, Zhanghui Zeng, Ning Han, Yinong Yang, Muyuan Zhu, Hongwu Bian
BACKGROUND: Vacuolar processing enzymes (VPEs) are cysteine proteinases that act as crucial mediators of programmed cell death (PCD) in plants. In rice, however, the role of VPEs in abiotic stress-induced PCD remains largely unknown. In this study, we generated OsVPE3 overexpression and suppression transgenic lines to elucidate the function of this gene in rice. RESULTS: Survival rate and chlorophyll retention analyses showed that suppression of OsVPE3 clearly enhanced salt stress tolerance in transgenic rice compared with wild type...
December 2016: Rice
https://www.readbyqxmd.com/read/27899930/ability-to-remove-na-and-retain-k-correlates-with-salt-tolerance-in-two-maize-inbred-lines-seedlings
#11
Yong Gao, Yi Lu, Meiqin Wu, Enxing Liang, Yan Li, Dongping Zhang, Zhitong Yin, Xiaoyun Ren, Yi Dai, Dexiang Deng, Jianmin Chen
Maize is moderately sensitive to salt stress; therefore, soil salinity is a serious threat to its production worldwide. Here, excellent salt-tolerant maize inbred line TL1317 and extremely salt-sensitive maize inbred line SL1303 were screened to understand the maize response to salt stress and its tolerance mechanisms. Relative water content, membrane stability index, stomatal conductance, chlorophyll content, maximum photochemical efficiency, photochemical efficiency, shoot and root fresh/dry weight, and proline and water soluble sugar content analyses were used to identify that the physiological effects of osmotic stress of salt stress were obvious and manifested at about 3 days after salt stress in maize...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27898807/identification-and-validation-of-candidate-genes-associated-with-domesticated-and-improved-traits-in-soybean
#12
Ling Zhou, Longhai Luo, Jian-Fang Zuo, Linfeng Yang, Li Zhang, Xuanmin Guang, Yuan Niu, Jianbo Jian, Qing-Chun Geng, Liping Liang, Qijian Song, Jim M Dunwell, Zhenzhen Wu, Jia Wen, Yu-Qin Liu, Yuan-Ming Zhang
Soybean, an important source of vegetable oils and proteins for humans, has undergone significant phenotypic changes during domestication and improvement. However, there is limited knowledge about genes related to these domesticated and improved traits, such as flowering time, seed development, alkaline-salt tolerance, and seed oil content (SOC). In this study, more than 106,000 single nucleotide polymorphisms (SNPs) were identified by restriction site associated DNA sequencing of 14 wild, 153 landrace, and 119 bred soybean accessions, and 198 candidate domestication regions (CDRs) were identified via multiple genetic diversity analyses...
July 2016: Plant Genome
https://www.readbyqxmd.com/read/27891145/contrasting-proteomic-and-metabolomic-responses-of-bermudagrass-to-drought-and-salt-stresses
#13
Tiantian Ye, Haitao Shi, Yanping Wang, Fan Yang, Zhulong Chan
Bermudagrass (Cynodon dactylon) is one of tolerant grass species to drought and salt. The comparative analyses of bermudagrass in response to drought and salt stresses at the physiological, proteomic, and metabolomic levels were performed in this study. The physiological results indicated that osmolytes accumulation, ROS level and antioxidant enzyme activities were extensively changed by drought and salt stresses. Through comparative proteomic analyses, we successfully identified a total of 77 proteins involved in photosynthesis, oxidative pentose phosphate, glycolysis, and redox metabolic pathways when exposed to drought and salt stresses...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27889145/potential-probiotic-pichia-kudriavzevii-strains-and-their-ability-to-enhance-folate-content-of-traditional-cereal-based-african-fermented-food
#14
Anna Greppi, Fabien Saubade, Cristian Botta, Christèle Humblot, Jean-Pierre Guyot, Luca Cocolin
With the aim of selecting starter cultures with interesting probiotic potential and with the ability to produce folate in a food matrix, yeast strains isolated from fermented cereal-based African foods were investigated. A total of 93 yeast strains were screened for their tolerance to pH 2 and 0.3% of bile salts. Pichia kudriavzevii isolates gave the best results. Selected P. kudriavzevii strains were tested for survival to the simulated human digestion and for adhesion to Caco-2 cells. Moreover, presence of folate biosynthesis genes was verified and production of extra and intra-cellular folate determined during growth in culture medium...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27888535/chitin-receptor-cerk1-links-salt-stress-and-chitin-triggered-innate-immunity-in-arabidopsis
#15
Catherine Espinoza, Yan Liang, Gary Stacey
In nature, plants need to respond to multiple environmental stresses that require involvement and fine-tuning of different stress signaling pathways. Cross-tolerance in which plants pre-treated with chitin (a fungal microbe-associated molecular pattern) have improved salt tolerance was observed in Arabidopsis but is not well understood. Here, we show a unique link between chitin and salt signaling mediated by the chitin receptor CHITIN ELICITOR RECEPTOR KINASE 1 (CERK1). Transcriptome analysis revealed that salt stress-induced genes are highly correlated with chitin-induced genes, while this was not observed with other microbe-associated molecular patterns (MAMP) or with other abiotic stresses...
November 26, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/27883150/metal-free-radical-thiolations-mediated-by-very-weak-bases
#16
Denis Koziakov, Michal Majek, Axel Jacobi von Wangelin
Aromatic thioethers and analogous heavier chalcogenides were prepared by reaction of arene-diazonium salts with disulfides in the presence of the cheap and weak base NaOAc. The mild and practical reaction conditions (equimolar reagents, DMSO, r.t., 8 h) tolerate various functional groups (e.g. Br, Cl, NO2, CO2R, OH, SCF3, furans). Mechanistic studies indicate the operation of a radical aromatic substitution mechanism via aryl, acetyloxyl, thiyl, and dimsyl radicals.
November 24, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27872576/the-alteration-of-mrna-expression-of-sod-and-gpx-genes-and-proteins-in-tomato-lycopersicon-esculentum-mill-under-stress-of-nacl-and-or-zno-nanoparticles
#17
Hesham F Alharby, Ehab M R Metwali, Michael P Fuller, Amal Y Aldhebiani
Five cultivars of tomato having different levels of salt stress tolerance were exposed to different treatments of NaCl (0, 3 and 6 g L(-1)) and ZnO-NPs (0, 15 and 30 mg L(-1)). Treatments with NaCl at both 3 and 6 g L(-1) suppressed the mRNA levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) genes in all cultivars while plants treated with ZnO-NPs in the presence of NaCl, showed increments in the mRNA expression levels. This indicated that ZnO-NPs had a positive response on plant metabolism under salt stress...
November 2016: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/27871319/a-phase-i-dose-escalation-study-of-pep02-irinotecan-liposome-injection-in-combination-with-5-fluorouracil-and-leucovorin-in-advanced-solid-tumors
#18
Nai-Jung Chiang, Tsu-Yi Chao, Ruey-Kuen Hsieh, Cheng-Hsu Wang, Yi-Wen Wang, C Grace Yeh, Li-Tzong Chen
BACKGROUND: PEP02 (also known as MM-398, nal-IRI) is a novel nanoparticle formulation of irinotecan encapsulated in liposomes. The aims of this study were to investigate the dose-limiting toxicity (DLT), maximum tolerated dose (MTD) and pharmacokinetics (PK) of PEP02 in combination with 5-FU and LV, in patients with advanced refractory solid tumors. METHODS: Patients were enrolled in cohorts to receive PEP02 from 60 to 120 mg/m(2) (dose expressed as the irinotecan hydrochloride trihydrate salt) as a 90-min intravenous infusion on day 1, followed by 24 h infusion of 5-FU 2,000 mg/m(2) and LV 200 mg/m(2) on days 1 and 8, every 3 weeks...
November 21, 2016: BMC Cancer
https://www.readbyqxmd.com/read/27870905/mapping-qtl-for-sex-and-growth-traits-in-salt-tolerant-tilapia-oreochromis-spp-x-o-mossambicus
#19
Grace Lin, Elaine Chua, Laszlo Orban, Gen Hua Yue
In aquaculture, growth and sex are economically important traits. To accelerate genetic improvement in increasing growth in salt-tolerant tilapia, we conducted QTL mapping for growth traits and sex with an F2 family, including 522 offspring and two parents. We used 144 polymorphic microsatellites evenly covering the genome of tilapia to genotype the family. QTL analyses were carried out using interval mapping for all individuals, males and females in the family, respectively. Using all individuals, three suggestive QTL for body weight, body length and body thickness respectively were detected in LG20, LG22 and LG12 and explained 2...
2016: PloS One
https://www.readbyqxmd.com/read/27869212/the-erf-transcription-factor-family-in-cassava-genome-wide-characterization-and-expression-analyses-against-drought-stress
#20
Wei Fan, Meirong Hai, Yunling Guo, Zehong Ding, Weiwei Tie, Xupo Ding, Yan Yan, Yunxie Wei, Yang Liu, Chunlai Wu, Haitao Shi, Kaimian Li, Wei Hu
Cassava (Manihot esculenta) shows strong tolerance to drought stress; however, the mechanisms underlying this tolerance are poorly understood. Ethylene response factor (ERF) family genes play a crucial role in plants responding to abiotic stress. Currently, less information is known regarding the ERF family in cassava. Herein, 147 ERF genes were characterized from cassava based on the complete genome data, which was further supported by phylogenetic relationship, gene structure, and conserved motif analyses...
November 21, 2016: Scientific Reports
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