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https://www.readbyqxmd.com/read/29793047/transcript-profiling-of-salt-tolerant-tobacco-mutants-generated-via-mutation-breeding
#1
Özge Çelik, Aybüke Ekşioğlu, Enes Yağız Akdaş
The main aim of the study is to identify the genes differentially, predominantly or specifically expressed in salt tolerant tobacco mutants, improved from Akhisar 97 and İzmir Özbaş varieties via mutation breeding, with respect to unstressed control plants. Seven tobacco mutants which have different salt tolerance capacities were evaluated by Gene Fishing analysis. Under stress conditions differentially expressed 100 reproducible bands were identified (74 of up-regulated and 20 of down-regulated while 6 were unknown)...
May 21, 2018: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29792968/applicability-of-endochitinase-of-flavobacterium-johnsoniae-with-transglycosylation-activity-in-generating-long-chain-chitooligosaccharides
#2
Papa Rao Vaikuntapu, Mohan Krishna Mallakuntla, Subha Narayan Das, Bhoopal Bhuvanachandra, Bellamkonda Ramakrishna, Sandhya Rani Nadendla, Appa Rao Podile
Chitin and its derivatives are used for a variety of applications. Flavobacterium johnsoniae UW1 is an aerobic Gram-negative bacterium and genome analysis revealed the presence of 10 glycoside hydrolases (GHs) that may degrade or modify chitin. The gene encoding chitinase B (FjchiB), which encodes a single catalytic GH18 domain has been cloned and heterologously expressed in Escherichia coli. FjChiB was optimally active in 50 mM sodium citrate buffer (pH 6.0) at 40 °C. FjChiB was salt-tolerant and catalytically versatile, with substrate specificity towards 75% DDA (degree of de-acetylation) chitosan, followed by colloidal chitin...
May 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29791976/a-cipk-protein-kinase-targets-sucrose-transporter-mdsut2-2-at-ser-254-for-phosphorylation-to-enhance-salt-tolerance
#3
Qi-Jun Ma, Mei-Hong Sun, Hui Kang, Jing Lu, Chun-Xiang You, Yu-Jin Hao
Soil salinity is one of the major abiotic stressors that negatively affect crop growth and yield. Salt stress can regulate antioxidants and the accumulation of osmoprotectants. In the study, a sucrose transporter MdSUT2.2 was identified in apple. Overexpression of MdSUT2.2 gene increased salt tolerance in the transgenic apple, compared with the WT control 'Gala'. In addition, it was found that protein MdSUT2.2 was phosphorylated at Ser254 site in response to salt. A DUAL membrane yeast hybridization system through an apple cDNA library demonstrated that a protein kinase MdCIPK13 interacted with MdSUT2...
May 23, 2018: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29787377/the-effects-of-inulin-and-fructo-oligosaccharide-on-the-probiotic-properties-of-lactobacillus-spp-isolated-from-human-milk
#4
Şener Tulumoğlu, Belgin Erdem, Ömer Şimşek
This study aims to determine the effects of inulin and fructo-oligosaccharide (FOS) on the probiotic properties of five Lactobacillus spp. isolated from human milk. Lactobacillus spp. were isolated and identified, and the growth characteristics, acid and bile salt tolerance, antagonistic effects, and cholesterol assimilation of Lactobacillus strains were investigated in the presence of inulin and FOS. Lactobacillus casei L1 was able to utilize inulin and FOS as carbon source as well as glucose even other strains were able to use, including Lactobacillus rhamnosus GG...
May 22, 2018: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/29787281/palladium-catalyzed-ring-forming-alkene-aminoaroylation-of-unsaturated-hydrazones-and-sulfonamides
#5
Jun Chen, Meng-Nan Yang, Jia-Rong Chen, Wen-Jing Xiao
The first example of a Pd(OAc)2 -catalyzed ring-forming alkene aminoaroylation of unsaturated hydrazones and sulfonamides is described. This protocol features the use of diaryliodonium salts as both oxidants and aryl sources, thus enabling mild reaction conditions, good chemoselectivity, a broad substrate scope, and high functional group tolerance. A wide range of synthetically and biologically important functionalized dihydropyrazoles and isoxazolidines have been obtained in good yields.
May 22, 2018: Organic Letters
https://www.readbyqxmd.com/read/29785398/marker-assisted-introgression-of-saltol-qtl-enhances-seedling-stage-salt-tolerance-in-the-rice-variety-pusa-basmati-1
#6
Vivek Kumar Singh, Brahma Deo Singh, Amit Kumar, Sadhna Maurya, Subbaiyan Gopala Krishnan, Kunnummal Kurungara Vinod, Madan Pal Singh, Ranjith Kumar Ellur, Prolay Kumar Bhowmick, Ashok Kumar Singh
Marker-assisted selection is an unequivocal translational research tool for crop improvement in the genomics era. Pusa Basmati 1 (PB1) is an elite Indian Basmati rice cultivar sensitive to salinity. Here, we report enhanced seedling stage salt tolerance in improved PB1 genotypes developed through marker-assisted transfer of a major QTL, Saltol . A highly salt tolerant line, FL478, was used as the Saltol donor. Parental polymorphism survey using 456 microsatellite (SSR)/QTL-linked markers revealed 14.3% polymorphism between PB1 and FL478...
2018: International Journal of Genomics
https://www.readbyqxmd.com/read/29785071/identification-of-cis-regulatory-elements-associated-with-salinity-and-drought-stress-tolerance-in-rice-from-co-expressed-gene-interaction-networks
#7
Pragya Mishra, Nisha Singh, Ajay Jain, Neha Jain, Vagish Mishra, Pushplatha G, Kiran P Sandhya, Nagendra Kumar Singh, Vandna Rai
Rice, a staple food crop, is often subjected to drought and salinity stresses thereby limiting its yield potential. Since there is a cross talk between these abiotic stresses, identification of common and/or overlapping regulatory elements is pivotal for generating rice cultivars that showed tolerance towards them. Analysis of the gene interaction network (GIN) facilitates identifying the role of individual genes and their interactions with others that constitute important molecular determinants in sensing and signaling cascade governing drought and/or salinity stresses...
2018: Bioinformation
https://www.readbyqxmd.com/read/29778839/spermidine-mediated-hydrogen-peroxide-signaling-enhances-the-antioxidant-capacity-of-salt-stressed-cucumber-roots
#8
Jianqiang Wu, Sheng Shu, Chengcheng Li, Jin Sun, Shirong Guo
Hydrogen peroxide (H2 O2 ) is a key signaling molecule that mediates a variety of physiological processes and defense responses against abiotic stress in higher plants. In this study, our aims are to clarify the role of H2 O2 accumulation induced by the exogenous application of spermidine (Spd) to cucumber (Cucumis sativus) seedlings in regulating the antioxidant capacity of roots under salt stress. The results showed that Spd caused a significant increase in endogenous polyamines and H2 O2 levels, and peaked at 2 h after salt stress...
May 5, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29777263/folate-treatment-of-pregnant-rat-dams-abolishes-metabolic-effects-in-female-offspring-induced-by-a-paternal-pre-conception-unhealthy-diet
#9
Jian Li, Yong-Ping Lu, Oleg Tsuprykov, Ahmed A Hasan, Christoph Reichetzeder, Mei Tian, Xiao Li Zhang, Qin Zhang, Guo-Ying Sun, Jingli Guo, Mohamed M S Gaballa, Xiao-Ning Peng, Ge Lin, Berthold Hocher
AIMS/HYPOTHESIS: Paternal high-fat diet prior to mating programmes impaired glucose tolerance in female offspring. We examined whether the metabolic consequences in offspring could be abolished by folate treatment of either the male rats before mating or the corresponding female rats during pregnancy. METHODS: Male F0 rats were fed either control diet or high-fat, high-sucrose and high-salt diet (HFSSD), with or without folate, before mating. Male rats were mated with control-diet-fed dams...
May 18, 2018: Diabetologia
https://www.readbyqxmd.com/read/29775712/rheological-and-fracturing-characteristics-of-a-novel-sulfonated-hydroxypropyl-guar-gum
#10
Liewei Qiu, Yiding Shen, Tao Wang, Chen Wang
A series of sulfonated hydroxypropyl guar gum (SHG) samples with different degrees of substitution (DSs) were prepared, and the SHG solution and SHG fracturing fluid were prepared and analyzed. The SHG aqueous solutions with different DSs all exhibit shear thinning behavior, which is well correlated with the Ostwald-deWaele model. Owing to the electrostatic repulsion of SHG molecular chains, SHG solutions with a higher DS will exhibit weaker thixotropic performance and strong anti-salinity ability. In addition, the SHG fracturing fluids, which were formed by interactions between SHG and organic zirconium, exhibit good temperature- and shear-resistant properties, proppant suspension properties, and salt tolerance...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29773867/novel-haloarchaeon-natrinema-thermophila-having-the-highest-growth-temperature-among-haloarchaea-with-a-large-genome-size
#11
Yeon Bee Kim, Joon Yong Kim, Hye Seon Song, Changsu Lee, Seung Woo Ahn, Se Hee Lee, Min Young Jung, Jin-Kyu Rhee, Juseok Kim, Dong-Wook Hyun, Jin-Woo Bae, Seong Woon Roh
Environmental temperature is one of the most important factors for the growth and survival of microorganisms. Here we describe a novel extremely halophilic archaeon (haloarchaea) designated as strain CBA1119T isolated from solar salt. Strain CBA1119T had the highest maximum and optimal growth temperatures (66 °C and 55 °C, respectively) and one of the largest genome sizes among haloarchaea (5.1 Mb). It also had the largest number of strain-specific pan-genome orthologous groups and unique pathways among members of the genus Natrinema in the class Halobacteria...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29772493/tissue-physiological-metabolic-adaptability-in-young-and-old-leaves-of-reed-phragmites-communis-in-songnen-grassland
#12
Rui Guo, Zhenzi Bai, Ji Zhou, XiuLi Zhong, FengXue Gu, Qi Liu, HaoRu Li
Common reed (Phragmites communis) is widely distributed as the dominant plant species in the Songnen Plain of China. The aim of this study was to investigate different physiological adaptive mechanisms to salinity tolerance between young and old leaves. The profiles of 68 metabolites were measured and studied in reed leaves by gas chromatography-mass spectrometer. The nitrogen, carbon, and pigment contents showed stronger growth inhibition for older leaves with salinity stress. In young leaves, high K+ contents not only promoted cell growth, but also prevented influx of superfluous Na+ ions in cells; the Ca2+ accumulation in old leaves implied that Ca2+ triggered the SOS-Na+ exclusion system and reduced Na+ toxicity...
May 9, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29766351/comparative-whole-genome-re-sequencing-analysis-in-upland-new-rice-for-africa-insights-into-the-breeding-history-and-respective-genome-compositions
#13
Naoki Yamamoto, Richard Garcia, Tomohiro Suzuki, Celymar Angela Solis, Yuichi Tada, Ramaiah Venuprasad, Ajay Kohli
BACKGROUND: Increasing rice demand is one of the consequences of the steadily improving socio-economic status of the African countries. New Rice for Africa (NERICA), which are interspecific hybrids between Asian and African rice varieties, are one of successful breeding products utilizing biodiversity across the two different rice crop species. Upland NERICA varieties (NU) exhibit agronomic traits of value for the harsh eco-geography, including shorter duration, higher yield and stress tolerance, compared to local African varieties...
May 15, 2018: Rice
https://www.readbyqxmd.com/read/29763329/fullerene-cation-mediated-noble-metal-free-direct-introduction-of-functionalized-aryl-groups-onto-60-fullerene
#14
Xiao-Yu Yang, Hao-Sheng Lin, Il Jeon, Yutaka Matsuo
Aryl[60]fullerenyl cations (ArC60 + ), which were generated by heating aryl[60]fullerenyl dimers (ArC60 -C60 Ar) to generate aryl[60]fullerenyl radicals (ArC60 • ) followed by oxidation using Cu(II) salts (Cu(BF4 )2 (aq)), were reacted with various functionalized aryl boronic acids to produce functionalized 1,4-diaryl[60]fullerenes. This protocol tolerated various functional groups, such as OH, NH2 , COCH3 , and Cl substituents, with yields reaching 93%. C60 Ar1 Ar2 (Ar2 = p-NH2 C6 H4 ) was used as a dopant in a photoactive CH3 NH3 PbI3 layer of a perovskite solar cell...
May 15, 2018: Organic Letters
https://www.readbyqxmd.com/read/29762476/function-of-the-erfl1a-transcription-factor-in-wheat-responses-to-water-deficiency
#15
Tian Gao, Ge-Zi Li, Chuan-Ren Wang, Jie Dong, Sha-Sha Yuan, Yong-Hua Wang, Guo-Zhang Kang
The APETALA2/ethylene response factor (AP2/ERF) superfamily is involved in the responses of plants to biotic and abiotic stresses; however, the functions and mechanisms of some members of this family in plants are unclear. In our previous study, expression of TaERFL1a , a member of the AP2/ERF family, was remarkably induced in wheat seedlings suffering freezing stress. In this study, we show that its expression was rapidly upregulated in response to salt, cold, and water deficiency, suggesting roles in the responses to abiotic stresses...
May 15, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29761494/control-of-xylem-na-loading-and-transport-to-the-shoot-in-rice-and-barley-as-a-determinant-of-differential-salinity-stress-tolerance
#16
Tetsuya Ishikawa, Sergey Shabala
Control of xylem Na+ loading has often been named as the essential components of salinity tolerance mechanism. However, it is less clear to what extent the difference in this trait may determine differential salinity tolerance between species. In this study barley (Hordeum vulgare L. cv. CM72) and rice (Oryza sativa L. cv Dongjin) plants were grown under two levels of salinity. Na+ and K+ concentrations in the xylem sap, and shoot and root tissues were measured at different time points after stress onset. Salt-exposed rice plants prevented xylem Na+ loading for several days, but failed to control this process in the longer term, ultimately resulting in a massive Na+ shoot loading...
May 15, 2018: Physiologia Plantarum
https://www.readbyqxmd.com/read/29760074/atpep3-is-a-hormone-like-peptide-that-plays-a-role-in-the-salinity-stress-tolerance-of-plants
#17
Kentaro Nakaminami, Masanori Okamoto, Mieko Higuchi-Takeuchi, Takeshi Yoshizumi, Yube Yamaguchi, Yoichiro Fukao, Minami Shimizu, Chihiro Ohashi, Maho Tanaka, Minami Matsui, Kazuo Shinozaki, Motoaki Seki, Kousuke Hanada
Peptides encoded by small coding genes play an important role in plant development, acting in a similar manner as phytohormones. Few hormone-like peptides, however, have been shown to play a role in abiotic stress tolerance. In the current study, 17 Arabidopsis genes coding for small peptides were found to be up-regulated in response to salinity stress. To identify peptides leading salinity stress tolerance, we generated transgenic Arabidopsis plants overexpressing these small coding genes and assessed survivability and root growth under salinity stress conditions...
May 14, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29758530/arbuscular-mycorrhizal-fungi-alleviate-boron-toxicity-in-puccinellia-tenuiflora-under-the-combined-stresses-of-salt-and-drought
#18
Chunguang Liu, Zheng Dai, Mengying Cui, Wenkai Lu, Hongwen Sun
To investigate the effect of arbuscular mycorrhizal fungi (AMF) on boron (B) toxicity in plants under the combined stresses of salt and drought, Puccinellia tenuiflora was grown in the soil with the inoculation of Funneliformis mosseae and Claroideoglomus etunicatum. After three weeks of treatment, the plants were harvested to determine mycorrhizal colonization rates, plant biomass, as well as tissue B, phosphorus, sodium, and potassium concentrations. The results show that the combined stresses reduced mycorrhizal colonization...
May 11, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29758379/a-differential-tolerance-to-mild-salt-stress-conditions-among-six-italian-rice-genotypes-does-not-rely-on-na-exclusion-from-shoots
#19
Michele Bertazzini, Gian Attilio Sacchi, Giuseppe Forlani
Rice is very sensitive to salt stress at the seedling level, with consequent poor crop establishment. A natural variability in susceptibility to moderate saline environments was found in a group of six Italian temperate japonica rice cultivars, and the physiological determinants for salt tolerance were investigated. Cation (Na+ , K+ and Mg++ ) levels were determined in shoots from individual rice plantlets grown in the absence or in the presence of inhibitory, yet sublethal salt levels, and at increasing time after salt treatments...
April 27, 2018: Journal of Plant Physiology
https://www.readbyqxmd.com/read/29758378/enhanced-oxidative-stress-in-the-jasmonic-acid-deficient-tomato-mutant-def-1-exposed-to-nacl-stress
#20
Ibrahim Abouelsaad, Sylvie Renault
Jasmonic acid (JA) has been mostly studied in responses to biotic stresses, such as herbivore attack and pathogenic infection. More recently, the involvement of JA in abiotic stresses including salinity was highlighted; yet, its role in salt stress remained unclear. In the current study, we compared the physiological and biochemical responses of wild-type (WT) tomato (Solanum lycopersicum) cv Castlemart and its JA-deficient mutant defenseless-1 (def-1) under salt stress to investigate the role of JA. Plant growth, photosynthetic pigment content, ion accumulation, oxidative stress-related parameters, proline accumulation and total phenolic compounds, in addition to both enzymatic and non-enzymatic antioxidant activities, were measured in both genotypes after 14 days of 100 mM NaCl treatment...
April 21, 2018: Journal of Plant Physiology
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