Ruti Sella, Yamit Cohen-Tayar, Takako Noguchi, Emma N Finburgh, Rebecca R Lian, Anser A Abbas, Dominic F Hakim, Jennifer J Bu, Jiagang Zhao, Peter Shaw, Irit Bahar, Natalie A Afshari
Purpose: To compare the effect of three commonly prescribed anti-inflammatory eye drops on corneal epithelial cells in vitro. Methods: Three different lines of human corneal epithelial cells were tested: primary cells cultured from donor tissue, commercially available primary cells, and immortalized cells. Cells were seeded on 96-well plates and treated with the following eye drops: cyclosporine 0.05%, lifitegrast 5%, and tacrolimus 0.03% or 0.1%. Exposure times tested were 30 seconds, 1 minute, 2 minutes, 1 hour, 2 hours, 4 hours, and 24 hours...
September 1, 2022: Translational Vision Science & Technology