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https://www.readbyqxmd.com/read/29762031/thermostability-improvement-of-the-d-allulose-3-epimerase-from-dorea-sp-cag-317-by-site-directed-mutagenesis-at-the-interface-regions
#1
Wenli Zhang, Yanmin Zhang, Jiawei Huang, Ziwei Chen, Tao Zhang, Cuie Guang, Wanmeng Mu
D-Allulose is a low-calorie sweetener and has broad applications in the food, cosmetics and pharmaceutical industries. Recently, most studies focuse on D-allulose production from D-fructose by D-allulose 3-epimerase (DAEase). However, the major blocker of industrial production of D-allulose is the poor thermostability. In this study, site-directed mutagenesis at the interface regions of Dorea sp. DAEase was carried out, and the F154Y/E191D/I193F mutation was obtained. The mutant protein displayed much higher thermostability, with a t1/2 value of 20...
May 15, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29760482/glycemic-impact-of-non-nutritive-sweeteners-a-systematic-review-and-meta-analysis-of-randomized-controlled-trials
#2
Alexander D Nichol, Maxwell J Holle, Ruopeng An
BACKGROUND/OBJECTIVES: Nonnutritive sweeteners (NNSs) are zero- or low-calorie alternatives to nutritive sweeteners, such as table sugars. A systematic review and meta-analysis of randomized controlled trials was conducted to quantitatively synthesize existing scientific evidence on the glycemic impact of NNSs. SUBJECTS/METHODS: PubMed and Web of Science databases were searched. Two authors screened the titles and abstracts of candidate publications. The third author was consulted to resolve discrepancies...
May 15, 2018: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29755932/family-food-purchases-of-high-and-low-calorie-foods-in-full-service-supermarkets-and-other-food-retailers-by-black-women-in-an-urban-us-setting
#3
Benjamin W Chrisinger, Katherine Isselmann DiSantis, Amy E Hillier, Shiriki K Kumanyika
Public health interventions to increase supermarket access assume that shopping in supermarkets is associated with healthier food purchases compared to other store types. To test this assumption, we compared purchasing patterns by store-type for certain higher-calorie, less healthy foods (HCF) and lower-calorie, healthier foods (LCF) in a sample of 35 black women household shoppers in Philadelphia, PA. Data analyzed were from 450 food shopping receipts collected by these shoppers over four-week periods in 2012...
June 2018: Preventive Medicine Reports
https://www.readbyqxmd.com/read/29687583/regular-low-calorie-sweetener-consumption-is-associated-with-increased-secretion-of-glucose-dependent-insulinotropic-polypeptide
#4
Chee W Chia, Michelle Shardell, Kristofer S Gravenstein, Olga D Carlson, Eleanor M Simonsick, Luigi Ferrucci, Josephine M Egan
Low-calorie sweeteners (LCSs) are widely used for weight control despite limited evidence of their effectiveness and studies linking LCS consumption with incident obesity. We tested the hypothesis that regular LCS consumption is associated with higher postprandial glucose-dependent insulinotropic polypeptide (GIP) secretion, which has been linked to obesity. We used data from participants in the Baltimore Longitudinal Study of Aging who had completed a diet diary, had at least one visit during which they underwent an oral glucose tolerance test (OGTT), and had no diabetes...
April 23, 2018: Diabetes, Obesity & Metabolism
https://www.readbyqxmd.com/read/29684280/discovery-of-a-kojibiose-phosphorylase-in-escherichia-coli-k-12
#5
Keya W Mukherjee, Tamari Narindoshvili, Frank M Raushel
The substrate profiles for three uncharacterized enzymes (YcjM, YcjT, and YcjU) that are expressed from a cluster of twelve genes (ycjM-W and ompG) of unknown function in Escherichia coli K-12 have been determined. Through a comprehensive bioinformatic and steady-state kinetic analysis, the catalytic function of YcjT was determined to be kojibiose phosphorylase. In the presence of saturating phosphate and kojibiose (α-(1,2)-D-glucose-D-glucose) this enzyme catalyzes the formation of D-glucose and β-D-glucose-1-phosphate (kcat = 1...
April 23, 2018: Biochemistry
https://www.readbyqxmd.com/read/29680056/synthesis-of-rebaudioside-d-using-glycosyltransferase-ugtsl2-and-in-situ-udp-glucose-regeneration
#6
Liangliang Chen, Ping Sun, Fangfang Zhou, Yan Li, Kequan Chen, Honghua Jia, Ming Yan, Dachun Gong, Pingkai Ouyang
Steviol glycosides from Stevia rebaudiana leaves are used in stevia-based sweeteners for their intense sweetness and low calories. Rebaudioside D is present in leaves in minute quantities (∼0.4-0.5% w/w total dry weight), but it is ∼350 times sweeter than sucrose, and sweeter than the more abundant rebaudioside A and stevioside. In the present study, pathways for rebaudioside D synthesis and UDP-glucose recycling were developed by coupling recombinant UDP-glucosyltransferase UGTSL2 from Solanum lycopersicum and sucrose synthase StSUS1 from Solanum tuberosum...
September 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29660810/sensory-profile-drivers-of-liking-and-influence-of-information-on-the-acceptance-of-low-calorie-synbiotic-and-probiotic-chocolate-ice-cream
#7
Juliana Peres, Erick Esmerino, Alessandra Lins da Silva, Ilana Racowski, Helena Bolini
The objective of this study was to evaluate the sensory profile and the influence of the information on the acceptance of the symbiotic chocolate ice cream made with sucrose and different sweeteners (aspartame, sucralose, neotame, Stevia with 60%, 85%, 95%, and 97% of rebaudioside A) through analysis of variance (ANOVA), Tukey's test, and partial least of square (PLS) regression. Quantitative descriptive analysis (QDA) was carried out by 18 assessors, who evaluated the samples in relation to the raised descriptors...
April 16, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29659969/aspartame-consumption-for-12-weeks-does-not-affect-glycemia-appetite-or-body-weight-of-healthy-lean-adults-in-a-randomized-controlled-trial
#8
Kelly A Higgins, Robert V Considine, Richard D Mattes
Background: Low-calorie sweeteners are often used to moderate energy intake and postprandial glycemia, but some evidence indicates that they may exacerbate these aims. Objective: The trial's primary aim was to assess the effect of daily aspartame ingestion for 12 wk on glycemia. Effects on appetite and body weight were secondary aims. Methods: One hundred lean [body mass index (kg/m2): 18-25] adults aged 18-60 y were randomly assigned to consume 0, 350, or 1050 mg aspartame/d (ASP groups) in a beverage for 12 wk in a parallel-arm design...
April 1, 2018: Journal of Nutrition
https://www.readbyqxmd.com/read/29652579/assessment-of-the-contribution-of-dietary-and-beverage-intake-quality-to-obesity-development
#9
Saniye Bilici, Hande Mortaş, Betül Kocaadam, Eda Köksal
OBJECTIVE: Low dietary quality is an important indicator of unhealthy eating patterns that can lead to some consequences such as obesity, so policy is a very powerful tool that can affect the consumption of both healthy and unhealthy foods. Indices that assess whether nutritional policies are applied contribute to the assessment of the quality of the population's diet. This study was conducted to investigate the quality of diets and beverages consumed by Turkish adults, and the factors affecting them...
April 13, 2018: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/29543782/low-no-calorie-sweeteners-a-review-of-global-intakes
#10
REVIEW
Danika Martyn, Maryse Darch, Ashley Roberts, Han Youl Lee, Tina Yaqiong Tian, Naoko Kaburagi, Pablo Belmar
The current review examined published data on the intake of all major low-/no-calorie sweeteners-aspartame, acesulfame-K, saccharin, sucralose, cyclamate, thaumatin and steviol glycosides-globally over the last decade. The most detailed and complex exposure assessments were conducted in Europe, following a standardized approach. Japan and Korea similarly had up-to-date and regular intake data available. The data for other Asian countries, Latin America, Australia/New Zealand and global estimates, evaluated by the Joint FAO/WHO Expert Committee on Food Additives (JECFA), while available, were shown to be more limited in terms of design...
March 15, 2018: Nutrients
https://www.readbyqxmd.com/read/29476623/the-influence-of-adding-spices-to-reduced-sugar-foods-on-overall-liking
#11
John C Peters, Ryan Marker, Zhaoxing Pan, Jeanne Anne Breen, James O Hill
Reducing sugar intake is a major public health goal but many consumers are reluctant to use low calorie sweeteners. Two studies were conducted in healthy adults aged 18 to 65 to investigate whether addition of culinary spices to foods reduced in sugar could preserve hedonic liking. Test foods, black tea, oatmeal, and apple crisp, were prepared in full sugar (FS), reduced sugar (RS), and reduced sugar with spice (RSS) versions. Sugar reductions were 100%, 35%, and 37% for tea, oatmeal, and apple crisp, respectively...
March 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29458115/effect-of-acek-acesulfame-potassium-on-brain-function-under-dietary-restriction-in-mice
#12
Daisuke Ibi, Fumika Suzuki, Masayuki Hiramatsu
People preferably take zero or low-calorie beverages and foods with artificial sweeteners even though it has been recently suggested that long-term artificial sweetener use affects physiological functions. In addition, a lower body weight was considered to be more healthful, but an abnormally low body weight caused by an excessive diet has been reported to cause health problems. Acesulfame potassium (AceK) is one of the most commonly used for foods and beverages because of its resistance to thermal degradation and marked sweetness...
May 1, 2018: Physiology & Behavior
https://www.readbyqxmd.com/read/29455781/the-truth-about-artificial-sweeteners-are-they-good-for-diabetics
#13
EDITORIAL
Vikas Purohit, Sundeep Mishra
Artificial sweeteners are thought to be beneficial for diabetics or obese where refined sugar can be a problem. These low-calorie sweeteners are seemingly safe to use, provide sweetness without calories, and provide a choice of sweet foods to those who otherwise cannot partake them (refined sugars). However, while artificial sweeteners may indeed restrict calories most of them have no beneficial effects on control of diabetes mellitus; rather possibly increase its risk. Additionally, there could be some other safety concerns possibly risk of cancer...
January 2018: Indian Heart Journal
https://www.readbyqxmd.com/read/29455757/federal-nutrition-program-revisions-impact-low-income-households-food-purchases
#14
Shu Wen Ng, Bridget A Hollingsworth, Emily A Busey, Julie L Wandell, Donna R Miles, Jennifer M Poti
INTRODUCTION: The Special Supplemental Nutrition Program for Women, Infants and Children (WIC) required major revisions to food packages in 2009; effects on nationwide low-income household purchases remain unexamined. METHODS: This study examines associations between WIC revisions and nutritional profiles of packaged food purchases from 2008 to 2014 among 4,537 low-income households with preschoolers in the U.S. (WIC participating versus nonparticipating) utilizing Nielsen Homescan Consumer Panel data...
March 2018: American Journal of Preventive Medicine
https://www.readbyqxmd.com/read/29438844/top-down-expectation-effects-of-food-labels-on-motivation
#15
Joost Wegman, Ilke van Loon, Paul A M Smeets, Roshan Cools, Esther Aarts
Labels on food packages inform our beliefs, shaping our expectations of food properties, such as its expected taste and healthiness. These beliefs can influence the processing of caloric rewards beyond objective sensory properties and have the potential to impact decision making. However, no studies, within or beyond the food domain, have assessed how written information, such as food labels, affect implicit motivation to obtain rewards, even though choices in daily life might be strongly driven by implicit motivational biases...
June 2018: NeuroImage
https://www.readbyqxmd.com/read/29433623/effects-of-life-cycle-and-leaves-location-on-gene-expression-and-glycoside-biosynthesis-pathway-in-stevia-rebaudiana-bertoni
#16
Matin Ghaheri, Elaheh Adibrad, Seyed Mehdi Safavi, Danial Kahrizi, Ali Soroush, Saare Muhammadi, Tayebeh Ghorbani, Ali Sabzevari, Zahra Ansarypour, Elham Rahmanian
Stevia rebaudiana Bertoni is One of the most important biologically sourced and low-calorie sweeteners that known as "Sweet Weed". It contains steviol glycosides that they are about 200-300 times sweeter than sucrose. Tissue culture is the best method with high efficiency that can overcome to problems of traditional methods, and it is the most useful tools for studying stress tolerance mechanisms under in vitro conditions to obtain drought tolerance. In the present research, we investigated the impact of life cycle, leaves location and the harvesting time on expression of UGT74G1 and UGT76G1 as well as steviol glycosides accumulation...
February 10, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29433621/effect-of-stevia-rebaudiana-bertoni-extract-on-sexual-dysfunction-in-streptozotocin-induced-diabetic-male-rats
#17
Matin Ghaheri, Shahram Miraghaee, Atefeh Babaei, Bahareh Mohammadi, Danial Kahrizi, Zahra Minoosh Saivosh Haghighi, Gholamreza Bahrami
Stevia rebaudiana Bertoni has been used locally as a non-calorie sweetener in medicine and diabetic diet which claimed to have aphrodisiac properties, although no scientific data of this function have been reported. The aim of this study was to investigate the effect of S. rebaudiana extract on sexual dysfunction, testosterone levels and number of Leydig cells in Streptozotocin (STZ)-induced diabetic male rats. A total of 28 diabetic male rats were randomly divided into 4 groups: diabetic group without any extract and 3 extract groups (5, 50 and 100 mg/kg)...
February 10, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29433620/effects-of-various-glutamine-concentrations-on-gene-expression-and-steviol-glycosides-accumulation-in-stevia-rebaudiana-bertoni
#18
Fatemeh Esmaeili, Matin Ghaheri, Danial Kahrizi, Mohsen Mansouri, Seyed Mehdi Safavi, Tayebeh Ghorbani, Sarre Muhammadi, Elham Rahmanian, Siavash Vaziri
Stevia rebaudiana Bertoni is one of the most important biologically sourced and low-calorie sweeteners that contains a lots of Steviol glycosides. Tissue culture is the best method for propagation of stevia and micro nutrients can affect both morphological traits and steviol glycosides production. In the present study, we investigated the effect of different concentrations of glutamine (10, 20, 30 and 40 g/l) on expression of UGT74G1 and UGT76G1 genes and stevioside and rebaudioside A accumulation in the leaves of stevia under in vitro conditions...
February 10, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29425973/metabolic-and-cognitive-improvement-from-switching-to-saccharin-or-water-following-chronic-consumption-by-female-rats-of-10-sucrose-solution
#19
Michael D Kendig, Michelle X Fu, Simone Rehn, Sarah I Martire, Robert A Boakes, Kieron B Rooney
High consumption of sugar-sweetened beverages (SSBs) is a risk factor for weight gain and metabolic disease. Whether this risk is reduced by switching to 'diet' beverages containing low-calorie sweeteners (LCS) is controversial. Two experiments modeled whether a switch from SSB to LCS beverages produced positive outcomes on behavioral and metabolic measures. Both experiments consisted of a Stage 1, in which adult female rats received unrestricted access to 10% sucrose solution in addition to chow and water for 4 (Experiment 1) or 8 weeks (Experiment 2)...
May 1, 2018: Physiology & Behavior
https://www.readbyqxmd.com/read/29389508/nutrition-facts-panels-who-uses-them-what-do-they-use-and-how-does-use-relate-to-dietary-intake
#20
Mary J Christoph, Nicole Larson, Melissa N Laska, Dianne Neumark-Sztainer
BACKGROUND: Nutrition labels are a low-cost tool with the potential to encourage healthy eating habits. OBJECTIVE: To investigate correlates of frequent Nutrition Facts label use, describe the types of label information most often used, and measure how label use relates to dietary intake in young adults. DESIGN: Cross-sectional population-based study of young adults participating in Project Eating and Activity in Teens and Young Adults-IV...
February 2018: Journal of the Academy of Nutrition and Dietetics
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