keyword
MENU ▼
Read by QxMD icon Read
search

gliadine

keyword
https://www.readbyqxmd.com/read/28737923/co-silencing-intestinal-transglutaminase-2-and-interleukin-15-using-gelatin-based-nanoparticles-in-an-in-vitro-model-of-celiac-disease
#1
Husain Attarwala, Valerie Clausen, Prasoon Chaturvedi, Mansoor M Amiji
In this study, we have developed type B gelatin-based nanoparticle based siRNA delivery system for silencing of intestinal transglutaminase-2 (TG2) and interleukin-15 (IL-15) genes in cultured human intestinal epithelial cells (Caco-2) and murine alveolar macrophage cells (J774A.1). Small interfering RNA (siRNA) targeting the TG2 or IL-15 gene was encapsulated within gelatin nanoparticles using ethanol-water solvent displacement method. Size, charge and morphology of gelatin nanoparticles were evaluated using Zetasizer instrument and transmission electron microscopy...
July 24, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28722361/-food-dependent-exercise-induced-anaphylaxis-a-stepwise-diagnosis
#2
Julien Gaillard, Amélie Borgeat-Kaeser, Guillaume Buss, François Spertini
Food-dependent exercise-induced anaphylaxis (FDEIA) is a potentially severe food allergy. Physical exercise, NSAID, alcohol, infectious diseases and estrogens are recognized cofactors, able to reduce the amount of allergen needed to achieve a threshold for the induction of anaphylaxis. Various kinds of causative food but only a few responsible proteins have been identified. The best known is wheat ω5-gliadine. An oral food challenge remains the gold standard to prove the diagnosis. Its clinical application remains difficult and includes an allergen challenge, a cofactor challenge and a third step which integrates both of them in a single test...
April 5, 2017: Revue Médicale Suisse
https://www.readbyqxmd.com/read/28721717/microbial-transglutaminase-used-in-bread-preparation-at-standard-bakery-concentrations-does-not-increase-immuno-detectable-amounts-of-deamidated-gliadin
#3
Andreas Heil, Jürgen Ohsam, Bernard van Genugten, Oscar Diez, Keiichi Yokoyama, Yoshiyuki Kumazawa, Ralf Pasternack, Martin Hils
The effect of standard bakery concentrations of microbial transglutaminase (MTG) in wheat bread preparation on the immunoreactivity of celiac disease (CD) patients' sera was investigated. Immunoblotting using monoclonal antibodies specific to unmodified and/or deamidated gliadin showed no differences between control bread and MTG-bread. Deamidation of gliadin could not be detected at standard MTG concentrations. CD patients' sera were characterized using anti-gliadin and anti-DGP ELISA and grouped into DGP high and low titer pools...
July 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28710588/identification-and-molecular-characterization-of-a-trans-acting-small-interfering-rna-producing-locus-regulating-leaf-rust-responsive-gene-expression-in-wheat-triticum-aestivum-l
#4
Summi Dutta, Dhananjay Kumar, Shailendra Jha, Kumble Vinod Prabhu, Manish Kumar, Kunal Mukhopadhyay
A novel leaf rust responsive ta-siRNA-producing locus was identified in wheat showing similarity to 28S rRNA and generated four differentially expressing ta-siRNAs by phasing which targeted stress responsive genes. Trans-acting-small interfering RNAs (Ta-siRNAs) are plant specific molecules generally involved in development and are also stress responsive. Ta-siRNAs identified in wheat till date are all responsive to abiotic stress only. Wheat cultivation is severely affected by rusts and leaf rust particularly affects grain filling...
July 14, 2017: Planta
https://www.readbyqxmd.com/read/28708086/celiac-disease-presenting-with-peripheral-neuropathy-in-children-a-case-report
#5
Alessandra Pacitto, Alessandra Paglino, Lorenza Di Genova, Alberto Leonardi, Edoardo Farinelli, Nicola Principi, Giuseppe di Cara, Susanna Esposito
Background: Clinically relevant neurological manifestations in children with celiac disease (CD) are unusual, especially when they are considered as signs of the onset of the disease. In this paper, a case of Guillain-Barrè syndrome (GBS) as the first manifestation of CD in a 23-month-old child is reported. Case presentation: We describe a case of CD onset with peripheral neuropathy in a 23-month-old Bulgarian boy presenting with a sudden refusal to walk and absence of deep tendon reflexes in both lower limbs...
July 14, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28702325/the-human-digestive-tract-has-proteases-capable-of-gluten-hydrolysis
#6
Sergio Gutiérrez, Jenifer Pérez-Andrés, Honorina Martínez-Blanco, Miguel Angel Ferrero, Luis Vaquero, Santiago Vivas, Javier Casqueiro, Leandro B Rodríguez-Aparicio
OBJECTIVE: To identify, purify, and characterize the proteins responsible for glutenase activity in the feces of healthy subjects and patients with celiac disease (CD). METHODS: Sixteen subjects were included in this study; 8 were healthy with no known food intolerances, and 8 were treated CD patients on a gluten-free diet. Fecal samples were homogenized, and precipitated proteins were purified by chromatography. Glutenase activity was evaluated by bioassays, zymography, and high-performance liquid chromatography with immunogenic 33-mer, 19-mer, and 13-mer gliadin peptides...
July 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/28700675/occupation-with-grain-crops-is-associated-with-lower-type-1-diabetes-incidence-registry-based-case-control-study
#7
Martin Haupt-Jorgensen, Erik Nielsen, Kåre Engkilde, Mia Lerche, Jesper Larsen, Karsten Buschard
Intranasal administration of gliadin prevents autoimmune diabetes in non-obese diabetic mice. The current study was designed to investigate if bakers are intranasally exposed to gluten during work and whether occupation as baker is inversely associated with type 1 diabetes. Gliadin was measured in nasal swabs from eight bakers and butchers. The odds ratio of type 1 diabetes in selected profession groups was analysed in a registry-based case-control study with data from 1980 to 2010 derived from Statistics Denmark...
2017: PloS One
https://www.readbyqxmd.com/read/28687275/avoidance-of-cow-s-milk-based-formula-for-at-risk-infants-does-not-reduce-development-of-celiac-disease-a-randomized-controlled-trial
#8
Mila Hyytinen, Erkki Savilahti, Suvi M Virtanen, Taina Härkönen, Jorma Ilonen, Kristiina Luopajärvi, Raivo Uibo, Outi Vaarala, Hans K Åkerblom, Mikael Knip
BACKGROUND & AIMS: Feeding during first months of life might affect risk of celiac disease. Individuals with celiac disease or type 1 diabetes have been reported to have high titers of antibodies against cow´s milk proteins. Avoidance of cow's milk-based formula for infants with genetic susceptibility for type 1 diabetes reduced the cumulative incidence of diabetes-associated autoantibodies. We performed a randomized controlled trial, in the same population, to study whether weaning to an extensively hydrolyzed formula reduced the risk of celiac disease autoimmunity or celiac disease...
July 4, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28675098/production-of-a-novel-wheat-gluten-hydrolysate-containing-dipeptidyl-peptidase-iv-inhibitory-tripeptides-using-ginger-protease
#9
Yuki Taga, Osamu Hayashida, Masashi Kusubata, Kiyoko Ogawa-Goto, Shunji Hattori
Wheat gluten is a Pro-rich protein complex comprising glutenins and gliadins. Previous studies have reported that oral intake of enzymatic hydrolysates of gluten has beneficial effects, such as suppression of muscle injury and improvement of hepatitis. Here, we utilized ginger protease that preferentially cleaves peptide bonds with Pro at the P2 position to produce a novel type of wheat gluten hydrolysate. Ginger protease efficiently hydrolyzed gluten, particularly under weak acidic conditions, to peptides with an average molecular weight of <600 Da...
July 4, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28672109/effect-of-barley-%C3%AE-glucan-on-the-gluten-polymerization-process-in-dough-during-heat-treatment
#10
Ze-Hua Huang, Yang Zhao, Ke-Xue Zhu, Xiao-Na Guo, Wei Peng, Hui-Ming Zhou
Barley (Hordeum vulgare L.) β-glucan (BBG) is of interest as a result of its health benefits, but BBG presents significant disruptions on the gluten network, with a negative impact on food texture. To clarify the interaction between BBG and gluten in dough, the dynamic rheological, thermochemical process of gluten and microstructure of dough with BBG during heating were detected. The results showed that BBG delayed the gluten thermopolymerization reaction during heating and affected polymerization of specific molecular weight protein subunits...
July 13, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28664699/antibody-reactivity-in-patients-with-ige-mediated-wheat-allergy-to-various-subunits-and-fractions-of-gluten-and-non-gluten-proteins-from-%C3%AF-gliadin-free-wheat-genotypes
#11
Andrzej Skoczowski, Krystyna Obtułowicz, Ewa Czarnobilska, Wojciech Dyga, Marcel Mazur, Iwona Stawoska, Jacek Waga
[b]Abstract Introduction and objective[/b]. Gluten proteins (gliadins and glutenins) are polymorphic wheat storage proteins of allergenic properties. Significant differences in chemical composition between both protein groups allow to expect highly specific immunological response of individual subunits and fractions in reactions with IgE sera of people allergic to wheat. The aim of these studies was to identify and characterize the most allergenic gluten proteins (GP) and nongluten proteins (NGP) occurred in two closely related wheat hybrid genotypes...
May 11, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28660745/prolidase-is-a-critical-enzyme-for-complete-gliadin-digestion-in-tenebrio-molitor-larvae
#12
Valeriia F Tereshchenkova, Irina A Goptar, Dmitry P Zhuzhikov, Mikhail A Belozersky, Yakov E Dunaevsky, Brenda Oppert, Irina Yu Filippova, Elena N Elpidina
Prolidase is a proline-specific metallopeptidase that cleaves imidodipeptides with C-terminal Pro residue. Prolidase was purified and characterized from the Tenebrio molitor larval midgut. The enzyme was localized in the soluble fraction of posterior midgut tissues, corresponding to a predicted cytoplasmic localization of prolidase according to the structure of the mRNA transcript. Expression of genes encoding prolidase and the major digestive proline-specific peptidase (PSP)-dipeptidyl peptidase 4-were similar...
June 29, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28651807/safety-evaluation-of-transgenic-low-gliadin-wheat-in-sprague-dawley-rats-an-alternative-to-the-gluten-free-diet-with-no-subchronic-adverse-effects
#13
Carmen Victoria Ozuna, Francisco Barro
Gluten-associated pathologies have increased in recent years and there is a greater demand for low or gluten-free products. Transgenic low-gliadin wheat lines showed low T-cell response, good bread-making properties, and excellent sensory assets. The aim of this study was to evaluate the safety of the whole-wheat flour from one transgenic low-gliadin line (named E82) in a 90-day feeding study. In this study males (n = 50) and females (n = 50) SD rats were used. They were fed with doses of 1.42, 2.83 and 5...
June 23, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28631048/celiac-disease-from-etiological-factors-to-evolving-diagnostic-approaches
#14
REVIEW
Anantdeep Kaur, Olga Shimoni, Michael Wallach
Celiac disease has advanced from a medical rarity to a highly prevalent disorder. Patients with the disease show varying degrees of chronic inflammation within the small intestine due to an aberrant immune response to the digestion of gliadin found in wheat. As a result, cytokines and antibodies are produced in celiac patients that can be used as specific biomarkers for developing diagnostic tests. This review paper describes celiac disease in terms of its etiological cause, pathological effects, current diagnostic tests based on mucosal biopsy, and the genetic basis for the disease...
June 19, 2017: Journal of Gastroenterology
https://www.readbyqxmd.com/read/28628250/grain-subproteome-responses-to-nitrogen-and-sulfur-supply-in-diploid-wheat-triticum-monococcum-ssp-monococcum
#15
Titouan Bonnot, Emmanuelle Bancel, David Alvarez, Marlène Davanture, Julie Boudet, Marie Pailloux, Michel Zivy, Catherine Ravel, Pierre Martre
Wheat grain storage proteins (GSPs) make up most of the protein content of grain and determine flour end-use value. The synthesis and accumulation of GSPs depend highly on nitrogen (N) and sulfur (S) availability and it is important to understand the underlying control mechanisms. Here we studied how the einkorn (Triticum monococcum ssp. monococcum) grain proteome responds to different amounts of N and S supply during grain development. GSP composition at grain maturity was clearly impacted by nutrition treatments, due to early changes in the rate of GSP accumulation during grain filling...
June 19, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28627070/lack-of-immunogenicity-of-hydrolysed-wheat-flour-in-patients-with-coeliac-disease-after-a-short-term-oral-challenge
#16
R Mandile, S Picascia, C Parrella, A Camarca, M Gobbetti, L Greco, R Troncone, C Gianfrani, R Auricchio
BACKGROUND: A gluten-free diet is currently the only reliable therapeutic strategy that is approved for coeliac disease (CD). For many patients, however, compliance remains inadequate. AIM: To investigate the immunogenicity of wheat flour that was pre-treated with selected lactobacilli and fungal proteases (hydrolysed wheat gluten) in coeliac patients. METHODS: The immunogenicity of hydrolysed wheat gluten was evaluated both in vitro in intestinal T cell lines (TCLs) and in vivo in treated CD patients after a short-term gluten challenge...
August 2017: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28625740/comparative-proteomic-analysis-of-two-transgenic-low-gliadin-wheat-lines-and-non-transgenic-wheat-control
#17
María Dolores García-Molina, Vera Muccilli, Rosaria Saletti, Salvatore Foti, Stefania Masci, Francisco Barro
Gluten proteins are major determinants of the bread making quality of wheat, but also of important wheat-related disorders, including coeliac disease (CD), and allergies. We carried out a proteomic study using the total grain proteins from two low-gliadin wheat lines, obtained by RNAi, and the untransformed wild type as reference. The impact of silencing on both target and on non-target proteins was evaluated. Because of the great protein complexity, we performed separate analyses of four kernel protein fractions: gliadins and glutenin subunits, and metabolic and CM-like proteins, by using a classical 2D electrophoresis gel based approach followed by RP-HPLC/nESI-MS/MS...
June 15, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28624578/accuracy-in-diagnosis-of-celiac-disease-without-biopsies-in-clinical-practice
#18
K J Werkstetter, I R Korponay-Szabó, A Popp, V Villanacci, M Salemme, G Heilig, S T Lillevang, M L Mearin, C Ribes-Koninckx, A Thomas, R Troncone, B Filipiak, M Mäki, J Gyimesi, M Najafi, J Dolinšek, S Dydensborg Sander, R Auricchio, A Papadopoulou, A Vécsei, P Szitanyi, E Donat, R Nenna, Ph Alliet, F Penagini, H Garnier-Lengliné, G Castillejo, K Kurppa, R Shamir, A C Hauer, F Smets, S Corujeira, M van Winckel, S Buderus, S Chong, S Husby, S Koletzko, Piotr Socha, Bozena Cukrowska, Hania Szajewska, Jan Wyhowski, Nailah Brown, Gauri Batra, Zrinjka Misak, Sven Seiwerth, Yulia Dmitrieva, Dmitry Abramov, Yvan Vandenplas, Annieta Goossens, Maaike W Schaart, V T H B M Smit, Nicolas Kalach, Pierre Gosset, Judit B Kovács, Anikó Nagy, Ilona Lellei, Rita Kőbányai, Katayoun Khatami, Maryam Monajemzadeh, Konstantina Dimakou, Amalia Patereli, Tine Plato Hansen, Rajko Kavalar, Miguel Bolonio, Hubert Kogler, Gabriele Amann, Roberta Kosova, Mariantonia Maglio, Elke Janssens, Ruth Achten, Pavel Frűhauf, Helena Skálová, Thomas Kirchner, Laura Petrarca, Fabio Massimo Magliocca, Francesc Martínez, Vanesa Morente, Sonja Thanner-Lechner, Manfred Ratschek, Marco Gasparetto, Liz Hook, Danielle Canioni, Catherine Wanty, Anne Mourin, Kaija Laurila, Martine Vornane, Vered Nachmias Friedler, Sara L Morgenstern, Jorge Amil Dias, Fátima Carneiro, Stephanie Van Biervliet, Saskia Vande Velde, Hany Banoub, Steve Sampson, Annette M Müller, Adina Ene, Mandana Rafeey, Iran Amir Taher Eftekhar Sadat
BACKGROUND & AIMS: The guidelines of the European Society of Pediatric Gastroenterology, Hepatology, and Nutrition allow for diagnosis of celiac disease without biopsies in children with symptoms and levels of immunoglobulin A against tissue-transglutaminase (TGA-IgA) 10-fold or more the upper limit of normal (ULN), confirmed by detection of endomysium antibodies (EMA) and positivity for HLA-DQ2/DQ8. We performed a large, international prospective study to validate this approach. METHODS: We collected data from consecutive pediatric patients (18 years or younger) on a gluten-containing diet who tested positive for TGA-IgA from November 2011 through May 2014, seen at 33 pediatric gastroenterology units in 21 countries...
June 15, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28614732/influence-of-the-drug-distribution-in-electrospun-gliadin-fibers-on-drug-release-behavior
#19
Ying Xu, Jiao-Jiao Li, Deng-Guang Yu, Gareth R Williams, Jun-He Yang, Xia Wang
Drug distribution within its carrier in a solid dosage form often generates a profound influence on its release profile, particularly when the physicochemical properties of the carrier are exploited to manipulate drug release behavior. In this job, two different types of distributions of a model drug ibuprofen (IBU) within a protein gliadin in their electrospun nanofibers were intentionally created. One was homogeneous distribution in the monolithic fibers fabricated using a modified coaxial process, and the other one was heterogeneous distribution in the core/shell fibers prepared through a traditional coaxial process...
August 30, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28555380/anti-gliadin-antibodies-in-breast-milk-from-celiac-mothers-on-a-gluten-free-diet
#20
María Roca, Sabine Lisa Vriezinga, Paula Crespo-Escobar, Renata Auricchio, David Hervás, Gemma Castillejo, Maria Carmen Mena, Isabel Polanco, Riccardo Troncone, Maria Luisa Mearin, Carmen Ribes-Koninckx
PURPOSE: To analyze the presence of total IgA and anti-gliadin antibodies (AGA) in BM from CD mothers who follow a gluten-free diet (GFD) and from mothers on a normal gluten-containing diet (ND). METHODS: 218 samples of mature milk were obtained at different months of lactation (1-6) from 83 mothers (2 or more samples per mother) from Italy (Naples), The Netherlands (Leiden) and Spain (Madrid, Valencia and Reus): 42 CD mothers on GFD for more than 2 years and 41 non-CD mothers on a ND...
May 29, 2017: European Journal of Nutrition
keyword
keyword
62395
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"