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https://www.readbyqxmd.com/read/28551855/characterization-of-a-novel-theme-c-glycoside-hydrolase-family-9-cellulase-and-its-cbm-chimeric-enzymes
#1
Cheng-Jie Duan, Ming-Yue Huang, Hao Pang, Jing Zhao, Chao-Xing Wu, Jia-Xun Feng
In bacterial cellulase systems, glycoside hydrolase family 9 (GH9) cellulases are generally regarded as the major cellulose-degrading factors besides GH48 exoglucanase. In this study, umcel9A, which was cloned from uncultured microorganisms from compost, with the encoded protein being theme C GH9 cellulase, was heterologously expressed in Escherichia coli, and the biochemical properties of the purified enzyme were characterized. Hydrolysis of carboxylmethylcellulose (CMC) by Umcel9A led to the decreased viscosity of CMC solution and production of reducing sugars...
May 27, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28551479/dna-and-protein-binding-double-strand-dna-cleavage-and-cytotoxicity-of-mixed-ligand-copper-ii-complexes-of-the-antibacterial-drug-nalidixic-acid
#2
Rangasamy Loganathan, Mani Ganeshpandian, Nattamai S P Bhuvanesh, Mallayan Palaniandavar, Amsaveni Muruganantham, Swapan K Ghosh, Anvarbatcha Riyasdeen, Mohammad Abdulkader Akbarsha
The water soluble mixed ligand complexes [Cu(nal)(diimine)(H2O)](ClO4) 1-4, where H(nal) is nalidixic acid and diimine is 2,2'-bipyridine (1), 1,10-phenanthroline (2), 5,6-dimethyl-1,10-phenanthroline (3), and 3,4,7,8-tetramethyl-1,10-phenanthroline (4), have been isolated. The coordination geometry around Cu(II) in 1 and that in the Density Functional Theory optimized structures of 1-4 has been assessed as square pyramidal. The trend in DNA binding constants (Kb) determined using absorption spectral titration (Kb: 1, 0...
May 3, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28551269/biochemical-properties-and-application-of-a-novel-%C3%AE-1-3-1-4-glucanase-from-paenibacillus-barengoltzii
#3
Bin Zhang, Yu Liu, Hongye Yang, Qiaojuan Yan, Shaoqing Yang, Zheng-Qiang Jiang, Shaozhen Li
A novel endo-β-1,3-1,4-glucanase gene (PbBglu16A) was cloned from Paenibacillus barengoltzii and heterogeneously expressed in Escherichia coli. The recombinant β-1,3-1,4-glucanase (PbBglu16A) was purified to homogeneity with a recovery yield of 78.6% and a specific activity of 431.8Umg(-1). The molecular mass of PbBglu16A was estimated to be 44.0kDa by SDS-PAGE. The optimal pH and temperature of PbBglu16A were 6.0 and 55°C, respectively. The enzyme was stable within pH 3.5-9.0 and up to 55°C. PbBglu16A exhibited high substrate specificity towards barley β-glucan, oat β-glucan and lichenin...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28551223/resistant-starch-variation-among-high-amylose-rice-varieties-and-its-relationship-with-apparent-amylose-content-pasting-properties-and-cooking-methods
#4
Ming-Hsuan Chen, Christine J Bergman, Anna M McClung, Jace D Everette, Rodante E Tabien
Resistant starch (RS), which is not hydrolyzed in the small intestine, has proposed health benefits. We evaluated 40 high amylose rice varieties for RS content in cooked rice and a 1.9-fold difference was found. Some varieties had more than two-fold greater RS content than a US long-grain intermediate-amylose rice. The high amylose varieties were grouped into four classes according to paste viscosity and gelatinization temperature based on genetic variants of the Waxy and Starch Synthase IIa genes, respectively...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28551210/nanoencapsulation-of-chia-seed-oil-with-chia-mucilage-salvia-hispanica-l-as-wall-material-characterization-and-stability-evaluation
#5
Camila de Campo, Priscilla Pereira Dos Santos, Tania Maria Haas Costa, Karina Paese, Silvia Stanisçuaski Guterres, Alessandro de Oliveira Rios, Simone Hickmann Flôres
In this study, chia seed oil was nanoencapsulated utilizing chia seed mucilage (CSM) as wall material. The viscosity, encapsulation efficiency, loading capacity, transmission electron microscopy, FT-IR spectroscopy and thermal properties of chia seed oil nanoparticles (CSO-NP) were performed after preparation. Particle size, zeta potential, span value, and pH of CSO-NP and oxidation stability of nanoencapsulated and unencapsulated oil were evaluated during 28days of storage at accelerated conditions (40°C)...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28550376/development-of-an-amber-compatible-transferable-force-field-for-poly-ethylene-glycol-ethers-glymes
#6
Nathalia S V Barbosa, Yong Zhang, Eduardo R A Lima, Frederico W Tavares, Edward J Maginn
An all-atom force field consistent with the general AMBER force field (GAFF) format for poly(ethylene glycol) dimethyl ether (diglyme or G2) was developed by fitting to experimental liquid densities and dielectric constants. Not surprisingly, the new force field gives excellent agreement with experimental liquid phase densities and dielectric constants over a wide temperature range. Other dynamic and thermodynamic properties of liquid G2 such as its self-diffusion coefficient, shear viscosity, and vaporization enthalpy were also calculated and compared to experimental data...
June 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/28549680/thiomers-impact-of-in-situ-cross-linkers-on-mucoadhesive-properties
#7
Hung Thanh Lam, Gintare Leonaviciute, Ožbej Zupančič, Andreas Bernkop-Schnürch
The aim of this study was to evaluate the impact of in situ cross-linkers on the gelling and mucoadhesive properties of thiomers. Polycarbophil-cysteine conjugate (PCP-cys) was synthesized by covalent attachment of l-cysteine to polycarbophil via amide bond formation mediated by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC) and N-hydroxysuccinimide (NHS) whereas in situ cross-linkers (PAA-cys-MNA) were synthesized by the same bond formation between poly(acrylic acid) (PAA) of 2.1-, 6-, and 15kDa and 2-((2-amino-2-carboxyethyl)disulfanyl)nicotinic acid (cys-MNA) used as ligand...
May 23, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28549408/pasting-rheological-and-retrogradation-properties-of-low-amylose-rice-starch-with-date-syrup
#8
Ibrahim O Mohamed, Jobe Babucurr
Effects of date syrup on pasting, rheological, and retrogradation properties of low-amylose rice starch were investigated using three levels of date syrup (starch:syrup 1:1, 1:2, or 1:3). Measurements were carried out using HR-2 Discovery Rheometer equipped with a pasting cell and parallel plate geometry. The pasting measurements showed that the peak viscosity of the control is significantly higher than the samples with date syrup (p < 0.05), while the final viscosity increases with increased date syrup levels...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/28548600/a-multi-spectroscopic-and-molecular-docking-approach-to-investigate-the-interaction-of-antiviral-drug-oseltamivir-with-ct-dna
#9
Neda Hosseinpour Moghadam, Sadegh Salehzadeh, Nahid Shahabadi, Reza Golbedaghi
The possible interaction between the antiviral drug oseltamivir and calf thymus DNA at physiological pH was studied by spectrophotometry, competitive spectrofluorimetry, differential pulse voltammogram (DPV), circular dichroism spectroscopy (CD), viscosity measurements, salt effect, and computational studies. Intercalation of oseltamivir between the base pairs of DNA was shown by a sharp increase in specific viscosity of DNA and a decrease of the peak current and a positive shift in differential pulse voltammogram...
May 26, 2017: Nucleosides, Nucleotides & Nucleic Acids
https://www.readbyqxmd.com/read/28548559/the-yucatan-minipig-tmj-disc-structure-function-relationships-support-its-suitability-for-human-comparative-studies
#10
Natalia Vapniarsky, Ashkan Aryaei, Boaz Arzi, Jerry C Hu, David C Hatcher, Kyriacos A Athanasiou
Frequent involvement of the disc in temporomandibular joint (TMJ) disorders warrants attempts to tissue engineer TMJ disc replacements. Physiologically, a great degree of similarity is seen between humans and farm pigs (FPs), but the pig's rapid growth confers a significant challenge for in vivo experiments. Minipigs have a slower growth rate and are smaller than FPs, but minipig TMJ discs have yet to be fully characterized. The objective of this study was to determine the suitability of the minipig for TMJ studies by extensive structural and functional characterization...
May 26, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28548538/overestimation-of-viscosity-by-the-green-kubo-method-in-a-dusty-plasma-experiment
#11
Zach Haralson, J Goree
The Green-Kubo (GK) method is widely used in simulations of strongly coupled plasmas to obtain the viscosity coefficient. However, the method's applicability, which is often taken for granted, has not been tested experimentally. We report an experimental test using a two-dimensional strongly coupled dusty plasma. We find that the GK viscosity is ≈60% larger than the result of a benchmark hydrodynamic method, obtained in the same experiment with the same conditions except for the presence of shear.
May 12, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28548523/strength-and-viscosity-effects-on-perturbed-shock-front-stability-in-metals
#12
S Opie, E Loomis, P Peralta, T Shimada, R P Johnson
Computational modeling and experimental measurements on metal samples subject to a laser-driven, ablative Richtmyer-Meshkov instability showed differences between viscosity and strength effects. In particular, numerical and analytical solutions, coupled with measurements of fed-through perturbations, generated by perturbed shock fronts onto initially flat surfaces, show promise as a validation method for models of deviatoric response in the postshocked material. Analysis shows that measurements of shock perturbation amplitudes at low sample thickness-to-wavelength ratios are not enough to differentiate between strength and viscosity effects, but that surface displacement data of the fed-through perturbations appears to resolve the ambiguity...
May 12, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28548517/experimental-determination-of-%C3%AE-s-for-finite-nuclear-matter
#13
Debasish Mondal, Deepak Pandit, S Mukhopadhyay, Surajit Pal, Balaram Dey, Srijit Bhattacharya, A De, Soumik Bhattacharya, S Bhattacharyya, Pratap Roy, K Banerjee, S R Banerjee
We present, for the first time, simultaneous determination of shear viscosity (η) and entropy density (s) and thus, η/s for equilibrated nuclear systems from A∼30 to A∼208 at different temperatures. At finite temperature, η is estimated by utilizing the γ decay of the isovector giant dipole resonance populated via fusion evaporation reaction, while s is evaluated from the nuclear level density parameter (a) and nuclear temperature (T), determined precisely by the simultaneous measurements of the evaporated neutron energy spectra and the compound nuclear angular momenta...
May 12, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28548165/on-the-underestimated-impact-of-the-gelation-temperature-on-macro-and-mesoporosity-in-monolithic-silica
#14
Rafael Meinusch, Rüdiger Ellinghaus, Kristof Hormann, Ulrich Tallarek, Bernd M Smarsly
The preparation of monolithic SiO2 with bimodal porosity using a special sol-gel procedure ("Nakanishi process") generally shows a pronounced sensitivity towards several physico-chemical parameters of the initial solution (concentrations, precursors, pH, temperature, etc.). Thus, temporal and spatial variations of these parameters during the sol-gel reactions can affect the final meso- and macropore space with respect to the pore size distributions and homogeneity. In this study we thoroughly examine the sol-gel reaction in terms of the impact of temperature accuracy and homogeneity during the gelation and their effect on meso- and macropore space...
May 26, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28547922/videofluoroscopic-assessment-of-the-pathophysiology-of-chronic-poststroke-oropharyngeal-dysphagia
#15
N Vilardell, L Rofes, V Arreola, A Martin, D Muriana, E Palomeras, O Ortega, P Clavé
BACKGROUND AND PURPOSE: Oropharyngeal dysphagia (OD) is a major complaint following stroke, associated with poor clinical outcome and high mortality rates. We aimed at characterizing the kinematics of swallow response associated with unsafe swallowing in chronic poststroke patients with OD. PATIENTS AND METHODS: Consecutive poststroke patients with a positive volume-viscosity swallow test for OD 3 months following stroke were studied by videofluoroscopy (VFS). Demographical and clinical factors and kinematics of swallow response were compared between those poststroke patients with safe swallow (penetration-aspiration scale, PAS≤2) and those with unsafe swallow (PAS≥3)...
May 25, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/28547182/three-year-survival-of-art-high-viscosity-glass-ionomer-and-resin-composite-restorations-in-people-with-disability
#16
Gustavo F Molina, Denise Faulks, Ignacio Mazzola, Ricardo J Cabral, Jan Mulder, Jo E Frencken
OBJECTIVES: To assess the 3-year cumulative survival rate of atraumatic restorative treatment (ART) and conventional resin composite restorations (CRT) placed in persons with disability. MATERIALS AND METHODS: Patients referred for restorative care to the Haemophilia Foundation special care service were treated by one of two specialists. Patients and/or caregivers were provided with written and verbal information regarding treatment options and selected the alternative they preferred...
May 25, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28547116/groove-binding-of-vanillin-and-ethyl-vanillin-to-calf-thymus-dna
#17
Kaixin Xia, Guowen Zhang, Song Li, Deming Gong
Vanillin (VAN) and ethyl vanillin (EVA) are widely used food additives as flavor enhancers, but may have a potential security risk. In this study, the properties of binding of VAN or EVA with calf thymus DNA (ctDNA) were characterized by multi-spectroscopic methods, multivariate curve resolution-alternating least-squares (MCR-ALS) algorithm and molecular simulation. The concentration profiles for the components (VAN or EVA, ctDNA and VAN-ctDNA or EVA-ctDNA complex) by the MCR-ALS analysis showed that VAN or EVA interacted with ctDNA and formed VAN-ctDNA or EVA-ctDNA complex...
May 25, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28546556/smelting-reduction-and-kinetics-analysis-of-magnetic-iron-in-copper-slag-using-waste-cooking-oil
#18
Bo Li, Xubin Wang, Hua Wang, Yonggang Wei, Jianhang Hu
To improve the recovery of copper, the viscosity of copper molten slag is decreased by the reduction of magnetic iron, which, in turn, accelerates the settling and separation of copper droplets from the slag. A new technology is proposed in which waste cooking oil is used as a reductant to reduce magnetic iron in the copper smelting slag and consequently reduce carbon emissions in the copper smelting process. A kinetic model of the reduction of magnetic iron in copper slag by waste cooking oil was built using experimental data, and the accuracy of the model was verified...
May 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28543050/effects-of-carrot-pomace-powder-and-a-mixture-of-pectin-and-xanthan-on-the-quality-of-gluten-free-batter-and-cakes
#19
Mahsa Majzoobi, Zahra Vosooghi Poor, Gholamreza Mesbahi, Jalal Jamalian, Asgar Farahnaky
Carrot pomace powder (CPP) is a valuable by-product of carrot processing containing nutrients and fibre and can be utilized for enrichment of gluten-free products. The main purpose of this study was to determine the effects of various levels of CPP (0, 10, 20 and 30%) and a mixture of hydrocolloids (HC) including pectin and xanthan (1.5% of each) on the quality of batter and gluten-free cakes. With increasing the level of CPP and inclusion of HC the viscosity of the batter increased significantly from 87 mPas for the control to > 7000 mPas for 30%CCP+HC sample...
May 20, 2017: Journal of Texture Studies
https://www.readbyqxmd.com/read/28542700/modeling-thrombus-formation-and-growth
#20
REVIEW
Hamid Hosseinzadegan, Danesh K Tafti
The paper reviews the state-of-the-art in computational modeling of thrombus formation and growth and related phenomena including platelet margination, activation, adhesion, and embolization. Presently, there is a high degree of empiricism in the modeling of thrombus formation. Based on the experimentally observed physics, the review gives useful strategies for predicting thrombus formation and growth. These include determining blood components involved in atherosclerosis, effective blood viscosity, tissue properties, and methods proposed for boundary conditions...
May 22, 2017: Biotechnology and Bioengineering
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