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https://www.readbyqxmd.com/read/28921619/alcohol-reduces-arterial-remodeling-by-inhibiting-sonic-hedgehog-stimulated-sca1-progenitor-stem-cell-expansion
#1
Emma Fitzpatrick, Xu Han, Weimin Liu, Eoin Corcoran, Denise Burtenshaw, Maryam Alshamrani, David Morrow, Jay-Christian Helt, Paul A Cahill, Eileen M Redmond
BACKGROUND: Cell and molecular mechanisms mediating the cardiovascular effects of alcohol are not fully understood. Our aim was to determine the effect of moderate Ethanol (EtOH) on Sonic Hedgehog (SHh) signaling in regulating possible Sca1(+) progenitor stem cell involvement during pathologic arterial remodeling. METHODS AND RESULTS: Partial ligation or sham-operation of the left carotid artery was performed in transgenic Sca1-eGFP mice gavaged with or without 'daily moderate' EtOH...
September 18, 2017: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/28917895/ultrasound-assisted-adsorptive-removal-of-hazardous-dye-safranin-o-from-aqueous-solution-using-crosslinked-graphene-oxide-chitosan-goch-composite-and-optimization-by-response-surface-methodology-rsm-approach
#2
Sushanta Debnath, Kamya Parashar, Kriveshini Pillay
Chitosan (CH) was crosslinked with graphene oxide (GO) by combining solutions of CH and GO. Characterisations by ATR-FTIR, FE-SEM and XRD confirmed the formation of the GOCH composite. Removal of the dye Safranin Orange (SO) by ultrasonic adsorption from aqueous solution was tested by the composite. The removal of the cationic dye was more favourable at pH values greater than 5.2 and the optimum pH was found to be 6.5. The adsorption kinetics followed a pseudo-first order model and the rate-limiting step was identified as boundary layer diffusion from the Intraparticle diffusion model...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917262/determination-of-several-element-levels-in-hardaliye-beverages-using-flame-atomic-absorption-spectrometry-after-ultrasound-probe-extraction
#3
Dilek Bakircioglu, Nukte Topraksever, Yasemin Bakircioglu Kurtulus
In the present study, concentrations of calcium (Ca), copper (Cu), magnesium (Mg), manganese (Mn), sodium (Na), and zinc (Zn) in hardaliye samples produced in Turkey were determined by flame atomic absorption spectrometry after ultrasound probe extraction (UPE), microwave-assisted extraction (MAE), and wet extraction procedures. At present, there is limited work in the literature on UPE for the determination of trace elements in beverage samples. Our single-correlation analysis showed that the elements studied with the UPE method in hardaliye were strongly correlated with the MAE procedure...
September 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28915233/the-utility-of-stem-cells-in-pediatric-urinary-bladder-regeneration
#4
REVIEW
Philip M Iannaccone, Vasil Galat, Matthew I Bury, Yongchao C Ma, Arun K Sharma
Pediatric patients with a neurogenic urinary bladder caused by developmental abnormalities including spina bifida exhibit chronic urological problems. Surgical management in the form of enterocystoplasty is used to enlarge the bladder but is associated with significant clinical complications. Thus, alternative methods to enterocystoplasty have been explored through the incorporation of stem cells with tissue engineering strategies. Within the context of this review, we will examine the use of bone marrow stem cells and induced pluripotent stem cells (iPSCs) as they relate to bladder regeneration at the anatomic and molecular levels...
September 15, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28914589/hplc-dad-method-development-and-validation-for-the-quantification-of-hydroxymethylfurfural-in-corn-chips-by-means-of-response-surface-optimization
#5
Sara Salvatierra Virgen, Silvia Guillermina Ceballos-Magaña, Vilma Del Carmen Salvatierra-Stamp, Maria Teresa Sumaya-Martínez, Francisco Javier Martínez-Martínez, Roberto Muñiz-Valencia
In recent years, there has been an increased concern about the presence of toxic compounds derived from the Maillard reaction produced during food cooking at high temperatures. The main toxic compounds derived from this reaction are acrylamide and hydroxymethylfurfural (HMF). The majority of analytical methods require sample treatments using solvents which are highly polluting for the environment. The difficulty for quantifying HMF in complex fried food matrices encourages the development of new analytical methods...
September 15, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28910676/porous-nh2-mil-125-as-an-efficient-nano-platform-for-drug-delivery-imaging-and-ros-therapy-utilized-low-intensity-visible-light-exposure-system
#6
Arunkumar Rengaraj, Pillaiyar Puthiaraj, Nam-Su Heo, Hoomin Lee, Seung Kyu Hwang, Soonjo Kwon, Wha-Seung Ahn, Yun-Suk Huh
Metal-organic frameworks are a novel class of organic-inorganic hybrid polymer with potential applications in bioimaging, drug delivery, and ROS therapy. NH2-MIL-125, which is a titanium-based metal organic framework with a large surface area of 1540m(2)/g, was synthesized using a hydrothermal method. The material was characterized by powder X-ray diffreaction (PXRD), thermogravimetric analysis (TGA), and transmission electron microscopy (TEM), and N2 isotherm analyses. The size of the polymer was reduced to the nanoscale using a high-frequency sonication process...
September 6, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28905123/no-difference-between-manual-and-different-power-toothbrushes-with-and-without-specific-instructions-in-young-oral-healthy-adults-results-of-a-randomized-clinical-trial
#7
Gerhard Schmalz, Katharina Kiehl, Jan Schmickler, Sven Rinke, Jana Schmidt, Felix Krause, Rainer Haak, Dirk Ziebolz
BACKGROUND: The aim of this randomized clinical study was to detect the effect of an instruction within a group using oscillating-rotating (OR), sonic-active (SA), or manual toothbrushes (MTB) in young, oral healthy adults. METHODS: One hundred fifty participants were randomly assigned into six groups (n = 25): with (OR-I, SA-I, MTB-I) and without instruction (OR-NI, SA-NI, MTB-NI). Participants in I subgroups received one standardized instruction of the toothbrush system...
September 13, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28902990/high-purity-semiconducting-single-walled-carbon-nanotubes-a-key-enabling-material-in-emerging-electronics
#8
Jacques Lefebvre, Jianfu Ding, Zhao Li, Paul Finnie, Gregory Lopinski, Patrick R L Malenfant
Semiconducting single-walled carbon nanotubes (sc-SWCNTs) are emerging as a promising material for high-performance, high-density devices as well as low-cost, large-area macroelectronics produced via additive manufacturing methods such as roll-to-roll printing. Proof-of-concept demonstrations have indicated the potential of sc-SWCNTs for digital electronics, radiofrequency circuits, radiation hard memory, improved sensors, and flexible, stretchable, conformable electronics. Advances toward commercial applications bring numerous opportunities in SWCNT materials development and characterization as well as fabrication processes and printing technologies...
September 13, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28901252/the-origin-and-identification-of-mesenchymal-stem-cells-in-teeth-from-odontogenic-to-non-odontogenic
#9
Dixin Cui, Hongyu Li, Mian Wan, Yiran Peng, Xin Xu, Xuedong Zhou, Liwei Zheng
Mesenchymal stem cells (MSCs) in teeth have been exploited as vital seed cells for stem cell-based dental medicine. To date, several mesenchymal stem cell populations originated from odontogenic tissue have been isolated and characterized by their expression of MSC surface markers and capacity of multi-lineage differentiation, including dental pulp stem cells (DPSCs), stem cells from human exfoliated deciduous teeth (SHED), stem cells from apical papilla (SCAP) and so on. However, their identity in vivo remains elusive, which hinders further understanding of their application in stem cell-based tooth regeneration...
September 13, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28898827/purification-and-antioxidant-activity-of-phycocyanin-from-synechococcus-sp-r42dm-isolated-from-industrially-polluted-site
#10
Ravi R Sonani, Stuti Patel, Bela Bhastana, Kinnari Jakharia, Mukesh G Chaubey, Niraj K Singh, Datta Madamwar
The cyanobacterium Synechococcus sp. R42DM, isolated from an industrially polluted site Vatva, Gujarat, India was recognized to produce phycocyanin (PC) as major phycobiliprotein. In present study, the combinatorial approach of chemical and physical methods i.e. Triton-X 100 treatment and ultra-sonication was designed for extraction of PC. From cell extract, the intact and functional-PC was purified up to purity 4.03 by ammonium sulphate fractionation and ion-exchange chromatography. The PC displayed considerable in vitro antioxidant and radical-scavenging activity...
August 31, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28898533/optimization-of-the-ultrasound-assisted-extraction-of-antioxidant-phloridzin-from-lithocarpus-polystachyus-rehd-using-response-surface-methodology
#11
Yang Chen, Li-Zi Yin, Ling Zhao, Gang Shu, Zhi-Xiang Yuan, Hua-Lin Fu, Cheng Lv, Ju-Chun Lin
The purpose of this study was to optimize the extraction process of phloridzin from Lithocarpus polystachyus Rehd. leaves using response surface methodology and to determine the antioxidant capacity of the extract. A Box-Behnken design was used to analyze the effects of ethanol concentration, liquid-solid ratio, soak time and extraction time on the extraction yield of phloridzin. The content of phloridzin was determined by high-performance liquid chromatography. To assess the antioxidant capacity of the extract, three in vitro test systems were used (1,1-,diphenyl-2-picrylhydrazyl, hydroxyl radical scavenging test and reduction force)...
September 12, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28894262/role-of-sonication-pre-treatment-and-cation-valence-in-the-sol-gel-transition-of-nano-cellulose-suspensions
#12
C A Maestri, M Abrami, S Hazan, E Chistè, Y Golan, J Rohrer, A Bernkop-Schnürch, M Grassi, M Scarpa, P Bettotti
Sol-gel transition of carboxylated cellulose nanocrystals has been investigated using rheology, SAXS, NMR and optical spectroscopies to unveil the distinctive roles of ultrasound treatments and addition of various cations. Besides cellulose fiber fragmentation, sonication treatment induces fast gelling of the solution. The gelation is independent of the addition of cations, while the final rheological properties are highly influenced by the type, concentration and sequence of the operations since the cations must be added prior to sonication to produce stiff gels...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28893250/association-between-dietary-patterns-and-cognitive-function-among-70-year-old-japanese-elderly-a-cross-sectional-analysis-of-the-sonic-study
#13
Hitomi Okubo, Hiroki Inagaki, Yasuyuki Gondo, Kei Kamide, Kazunori Ikebe, Yukie Masui, Yasumichi Arai, Tatsuro Ishizaki, Satoshi Sasaki, Takeshi Nakagawa, Mai Kabayama, Ken Sugimoto, Hiromi Rakugi, Yoshinobu Maeda
BACKGROUND: An increasing number of studies in Western countries have shown that healthy dietary patterns may have a protective effect against cognitive decline and dementia. However, information on this relationship among non-Western populations with different cultural settings is extremely limited. We aim to examine the relationship between dietary patterns and cognitive function among older Japanese people. METHODS: This cross-sectional study included 635 community-dwelling people aged 69-71 years who participated in the prospective cohort study titled Septuagenarians, Octogenarians, Nonagenarians Investigation with Centenarians (SONIC)...
September 11, 2017: Nutrition Journal
https://www.readbyqxmd.com/read/28892691/combined-pretreatment-of-electrolysis-and-ultra-sonication-towards-enhancing-solubilization-and-methane-production-from-mixed-microalgae-biomass
#14
Gopalakrishnan Kumar, Periyasamy Sivagurunathan, Guangyin Zhen, Takuro Kobayashi, Sang-Hyoun Kim, Kaiqin Xu
This study investigated the effect of combination of pretreatment methods such as ultra-sonication and electrolysis for the minimum energy input to recover the maximal carbohydrate and solubilization (in terms of sCOD) from mixed microalgae biomass. The composition of the soluble chemical oxygen demand (COD), protein, carbohydrate revealed that the hydrolysis method had showed positive impact on the increasing quantity and thus enhanced methane yields. As a result, the combination of these 2 pretreatments showed the greatest yield of soluble protein and carbohydrate as 279 and 309mg/L, which is the recovery of nearly 85 and 90% in terms of total content of them...
August 30, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28892064/a-mouse-model-for-embryonal-tumors-with-multilayered-rosettes-uncovers-the-therapeutic-potential-of-sonic-hedgehog-inhibitors
#15
Julia E Neumann, Annika K Wefers, Sander Lambo, Edoardo Bianchi, Marie Bockstaller, Mario M Dorostkar, Valerie Meister, Pia Schindler, Andrey Korshunov, Katja von Hoff, Johannes Nowak, Monika Warmuth-Metz, Marlon R Schneider, Ingrid Renner-Müller, Daniel J Merk, Mehdi Shakarami, Tanvi Sharma, Lukas Chavez, Rainer Glass, Jennifer A Chan, M Mark Taketo, Philipp Neumann, Marcel Kool, Ulrich Schüller
Embryonal tumors with multilayered rosettes (ETMRs) have recently been described as a new entity of rare pediatric brain tumors with a fatal outcome. We show here that ETMRs are characterized by a parallel activation of Shh and Wnt signaling. Co-activation of these pathways in mouse neural precursors is sufficient to induce ETMR-like tumors in vivo that resemble their human counterparts on the basis of histology and global gene-expression analyses, and that point to apical radial glia cells as the possible tumor cell of origin...
September 11, 2017: Nature Medicine
https://www.readbyqxmd.com/read/28892000/development-of-a-matrix-solid-phase-dispersion-extraction-combined-with-uplc-q-tof-ms-for-determination-of-phenolics-and-terpenoids-from-the-euphorbia-fischeriana
#16
Wenjing Li, Yu Lin, Yuchun Wang, Bo Hong
A method based on a simplified extraction by matrix solid phase dispersion (MSPD) followed by ultra-performance liquid chromatography coupled with the quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS) determination is validated for analysis of two phenolics and three terpenoids in Euphorbia fischeriana. The optimized experimental parameters of MSPD including dispersing sorbent (silica gel), ratio of sample to dispersing sorbent (1:2), elution solvent (water-ethanol: 30-70) and volume of the elution solvent (10 mL) were examined and set down...
September 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28891976/mechano-physical-properties-and-microstructure-of-carbon-nanotube-reinforced-cement-paste-after-thermal-load
#17
Maciej Szeląg
The article presents the results obtained in the course of a study on the use of carbon nanotubes (CNTs) for the modification of a cement matrix. Carbon nanotubes were introduced into a cement paste in the form of an aqueous dispersion in the presence of a surfactant (SDS-sodium dodecyl sulfate), which was sonicated. The selected physical and mechanical parameters were examined, and the correlations between these parameters were determined. An analysis of the local microstructure of the modified cement pastes has been carried out using scanning electron microscope (SEM) and X-ray microanalysis (EDS)...
September 11, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28891097/ventral-neural-patterning-in-the-absence-of-a-shh-activity-gradient-from-the-floorplate
#18
Angelo Iulianella, Daisuke Sakai, Hiroshi Kurosaka, Paul Trainor
BACKGROUND: Vertebrate spinal cord development requires Sonic Hedgehog (Shh) signaling from the floorplate and notochord, where it is thought to act in concentration dependent manner to pattern distinct cell identities along the ventral-to-dorsal axis. While in vitro experiments demonstrate naïve neural tissues are sensitive to small changes in Shh levels, genetic studies illustrate that some degree of ventral patterning can occur despite significant perturbations in Shh signaling. Consequently, the mechanistic relationship between Shh morphogen levels and acquisition of distinct cell identities remains unclear...
September 11, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28890550/electrochemical-fluorination-and-radiofluorination-of-methyl-phenylthio-acetate-using-tetrabutylammonium-fluoride-tbaf
#19
Mehrdad Balandeh, Christopher Waldmann, Daniela Shirazi, Adrian Gomez, Alejandra Rios, Nathanael Allison, Asad Khan, Saman Sadeghi
Electrochemical fluorination of methyl(phenylthio)acetate was achieved using tetrabutylammonium fluoride (TBAF). Electrochemical fluorination was performed under potentiostatic anodic oxidation using an undivided cell in acetonitrile containing TBAF and triflic acid. The influence of several parameters including: oxidation potential, time, temperature, sonication, TBAF concentration and triflic acid concentration on fluorination efficiency were studied. It was found that the triflic acid to TBAF concentration ratio plays a key role in the fluorination efficiency...
2017: Journal of the Electrochemical Society
https://www.readbyqxmd.com/read/28887094/salvianolic-acids-for-injection-safi-promotes-functional-recovery-and-neurogenesis-via-sonic-hedgehog-pathway-after-stroke-in-mice
#20
Ye Zhang, Xiangjian Zhang, Lili Cui, Rong Chen, Cong Zhang, Yaoru Li, Tingting He, Xingyuan Zhu, Zuyuan Shen, Lipeng Dong, Jingru Zhao, Ya Wen, Xiufen Zheng, Pan Li
There is a pressing need of developing approaches for delayed post-stroke therapy for patients who fail to receive thrombolysis within the narrow time window. Neuroprotection of Salvianolic Acids for Injection (SAFI) for cerebral ischemia-reperfusion injury in acute phase has been well documented. The current study was to determine the influence of SAFI at the subacute phase after stroke in mice, and to elucidate the underlying mechanisms. Adult male C57BL/6 mice were subjected to permanent occlusion of the distal middle cerebral artery (dMCAO), followed by daily intraperitoneal injection of SAFI 24 h after stroke for 14 days...
September 5, 2017: Neurochemistry International
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