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https://www.readbyqxmd.com/read/28544819/dual-malignancy-in-a-thyroid-papillary-thyroid-carcinoma-and-small-lymphocytic-lymphoma-a-report-of-a-case-with-a-cyto-histologic-correlation
#1
Omar I Ahmed, Ziyan T Salih
Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy. Simultaneous involvement of the thyroid gland by multiple malignancies, is a rare occurrence. Similarly, primary thyroid lymphomas are also rare. We are reporting a rare case of a dual thyroid malignancy; PTC with secondary thyroid involvement by chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), diagnosed on cytology and confirmed with flow cytometry, histology and immunohistochemistry. Imaging showed two hypermetabolic nodules, one in left parotid gland, and the other in the thyroid isthmus...
May 22, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28505590/apoptosis-induction-activity-and-molecular-docking-studies-of-survivin-sirna-carried-by-fe3o4-peg-lac-chitosan-pei-nanoparticles-in-mcf-7-human-breast-cancer-cells
#2
Sanam Arami, Majid Mahdavi, Mohammad-Reza Rashidi, Reza Yekta, Mohammad Rahnamay, Leila Molavi, Mohammad-Saeid Hejazi, Nasser Samadi
Delivery of small interfering RNAs (siRNAs) into cells still remains a challenge in gene delivery studies. Here, we investigated the ability of synthesized Fe3O4-PEG-LAC-chitosan-PEI nanoparticles for siRNA delivery of survivin as the model gene into cells. The cellular uptake of survivin siRNA carried by synthesized nanoparticles into MCF-7 breast cancer cell line was evaluated by florescent microscopy and flowcytometry, both proving the efficacy of nanoparticles in delivery of up to 64.7% in comparison with lipofectamine 2000...
May 6, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28502133/t-regulatory-cells-response-to-allergen-specific-immunotherapy-in-patients-with-allergic-airway-diseases-a-prospective-study
#3
Raghdaa A Ramadan, Emad A Morad, Mohamed El-Shabrawy
Forkhead box P3 (FoxP3) T regulatory (Treg) cells modulate the immune system by blocking other types of T-cells. They maintain tolerance to self-antigens and help in inducing tolerance to foreign antigens. A deregulation of FoxP3 Tregs seems to play an important role in allergic disorders. The aim of this work was to study the response of FoxP3 Treg cells and their FoxP3 expression in patients, attending the Allergy Unit and the Chest Outpatient Clinic, Faculty of Medicine, Zagazig University, with allergic airway diseases, before and 1 year after receiving subcutaneous allergen specific immunotherapy (SIT)...
June 2016: Egyptian Journal of Immunology
https://www.readbyqxmd.com/read/28496946/alpha-alumina-nanoparticle-conjugation-to-cysteine-peptidase-a-and-b-an-efficient-method-for-autophagy-induction
#4
Fatemeh Beyzay, Ahmad Zavaran Hosseini, Sara Soudi
BACKGROUND: Autophagy as a cellular pathway facilitates several immune responses against infection. It also eliminates invading pathogens through transferring content between the cytosol and the lysosomal vesicles and contributes to the cross-presentation of exogenous antigens to T lymphocytes via MHC class I pathway. Autophagy induction is one of the main targets for new drugs and future vaccine formulations. Nanoparticles are one of the candidates for autophagy induction. Cysteine Peptidase A (CPA) and Cysteine Peptidase B (CPB) are two members of papain family (Clan CA, family C1) enzyme that have been considered as a virulence factor of Leishmania (L...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496478/study-of-crocin-radiotherapy-induced-cytotoxicity-and-apoptosis-in-the-head-and-neck-cancer-hn-5-cell-line
#5
Vahid Vazifedan, Seyed Hadi Mousavi, Javad Sargolzaei, Shokouhozaman Soleymanifard, Azar Fani Pakdel
Malignant tumors of head and neck carcinomas are the sixth most common type of cancer. Current systemic therapies for cancer show side effects in normal tissues and short-term efficacy due to drug resistance. Consequently, there is much interest in identifying new drugs for cancer treatment. Crocin (an active ingredient of saffron) has been shown to have cytotoxic effects on cancer cell lines. Chemo radiotherapy is the standard treatment for head and neck cancer. In the present study, the cytotoxic effects, inducing apoptosis and the radiation sensitivity of crocin were evaluated in the head and neck cancer cell line (HN-5)...
2017: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/28484744/morphometric-characteristic-between-diploid-and-spontaneous-triploid-carp-in-korea
#6
Sang Gu Lim, Hyeng Keun Han, In Bon Goo, Hyun Woo Gil, Tae Ho Lee, In-Seok Park
We used flowcytometry to ploidy verification after that investigate difference between diploid and spontaneous triploid through the truss dimension and classical dimension at crucian carp, Carassius auratus, crucian carp C. cuvieri and common carp, Cyprinus carpio collected from Hangang river, Hantangang river, Imjingang river, Geumgang river, Yeongsangang river and Nakdonggang river, Korea. There were significant differences among the three species for the truss dimensions anterior origin of dorsal fin (2)× anterior origin of anal fin (5), 2× anterior origin of pelvic fin (6), 2× origin of pectoral fin (7), posterior origin of dorsal fin (3)×5, 3×6, and 3×7 (P<0...
March 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28454099/homoharringtonine-targets-smad3-and-tgf-%C3%AE-pathway-to-inhibit-the-proliferation-of-acute-myeloid-leukemia-cells
#7
Jian Chen, Qitian Mu, Xia Li, Xiufeng Yin, Mengxia Yu, Jing Jin, Chenying Li, Yile Zhou, Jiani Zhou, Shanshan Suo, Demin Lu, Jie Jin
Homoharringtonine (HHT) has long and widely been used in China for the treatment of acute myeloid leukemia (AML), the clinical therapeutic effect is significant but the working mechanism is poorly understood. The purpose of this study is to screen the possible target for HHT with virtual screening and verify the findings by cell experiments. Software including Autodock, Python, and MGL tools were used, with HHT being the ligand and proteins from PI3K-Akt pathway, Jak-stat pathway, TGF-β pathway and NK-κB pathway as the receptors...
April 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28453467/prognostic-significance-of-mycn-gene-amplification-and-protein-expression-in-primary-brain-tumors-astrocytoma-and-meningioma
#8
Mehrdad Asghari Estiar, Firouzeh Javan, Ali Zekri, Masoud Mehrazin, Parvin Mehdipour
BACKGROUND: Astrocytoma and meningioma are the most common primary brain tumors. MYCN as a member of MYC proto-oncogenes has recently appeared as an attractive therapeutic target. Functions of MYCN are critical for growth of nervous system and neural differentiation. OBJECTIVE: We examined MYCN amplification and protein expression in astrocytoma and meningioma cases. METHODS: In this study, we used real-time PCR, FISH assay and flowcytometry to analyze DNA amplification and protein expression of MYCN...
April 19, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28442350/extracellular-nampt-visfatin-induces-proliferation-through-erk1-2-and-akt-and-inhibits-apoptosis-in-breast-cancer-cells
#9
Zafar Gholinejad, Nejat Kheiripour, Mitra Nourbakhsh, Davod Ilbeigi, Kiarash Behroozfar, Zahra Hesari, Abolfazl Golestani, Mohammad Shabani, Nahid Einollahi
BACKGROUND: Visfatin is a novel adipokine and proinflammatory cytokine which is implicated in breast cancer progression. The exact proliferative and anti-apoptotic mechanisms of visfatin are still under debate. In this study, the effect of extracellular visfatin on proliferation and apoptosis of breast cancer cells were investigated considering key regulatory molecules in these procedures. METHODS: BrdU (Bromodeoxyuridine) experiment was used to assess cell proliferation in response to visfatin treatment...
April 23, 2017: Peptides
https://www.readbyqxmd.com/read/28395437/-the-preparation-and-evaluation-of-the-quality-control-materials-for-detection-of-platelet-membrane-glycoproteins-by-flow-cytometry
#10
Y Q Liu, Y Gong, C X Qu, R You, L P Li, L S Xing, J Z Wang
Objective: To prepare the quality control material for detection of platelet membrane glycoproteins by flowcytometry and evaluate the appearance traits, homogeneity and stability of it. Methods: Fresh platelets from the blood group O donors were fixed by the certain concentration of aldehyde solution and then washed by the imidazole buffer. After that, adding certain concentration of lyophilized protection solution into the preparations. The preparations were dispensed to be lyophilized and then were kept refrigerated in 2-8 ℃...
April 11, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28384114/immune-cell-subsets-evaluation-as-a-predictive-tool-for-hepatitis-b-infection-outcome-and-treatment-responsiveness
#11
Snezhina M Kandilarova, Atanaska I Georgieva, Anastasiya P Mihaylova, Marta P Baleva, Valentina K Atanasova, Diana V Petrova, Georgi T Popov, Elissaveta J Naumova
BACKGROUND: The patient's immune response is one of the major factors influencing HBV eradication or chronification, and it is thought to be responsible for the treatment success. AIM: Our study aimed to investigate whether cellular defense mechanisms are associated with the course of HBV infection (spontaneous recovery [SR] or chronification [CHB]) and with the therapeutic approach. PATIENTS AND METHODS: A total of 139 patients (118 with CHB, 21 SR) and 29 healthy individuals (HI) were immunophenotyped by flowcytometry...
March 1, 2017: Folia Medica
https://www.readbyqxmd.com/read/28380412/%C3%AE-asarone-promotes-temozolomide-s-entry-into-glioma-cells-and-decreases-the-expression-of-p-glycoprotein-and-mdr1
#12
Nanbu Wang, Qinxin Zhang, Baile Ning, Laiyu Luo, Yongqi Fang
Glioma is the most common primary brain tumor and has an undesirable prognosis due to the blood-brain barrier (BBB) and drug resistance. A thorough investigation of the changes in intracellular drug concentrations is important to observe therapeutic effects and cell resistance. P-glycoprotein (P-gp) is an essential protein of Multi-drug resistance 1 (MDR1). The over-expression of P-gp and MDR1 is associated with poor prognosis and drug-resistance in glioma. However, β-asarone can pass through the BBB easily and increase the drug concentration in the rat brain...
June 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28377223/generation-and-characterization-of-a-human-mouse-chimeric-high-affinity-antibody-that-detects-the-dykddddk-flag-peptide
#13
Koki Ikeda, Tomoaki Koga, Fumiyuki Sasaki, Ayumi Ueno, Kazuko Saeki, Toshiaki Okuno, Takehiko Yokomizo
DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. We recently established a high-affinity monoclonal antibody (mAb) for FLAG (clone 2H8). The 2H8 Ab is highly sensitive for detecting FLAG-tagged proteins by flowcytometry and immunoprecipitation, but it can yield nonspecific signals in immunohistochemistry of mouse tissues because it is of mouse origin. In this study, we reduced nonspecific signals by generating a chimeric 2H8 Ab with Fc fragments derived from human immunoglobulin...
May 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28375101/anti-proliferative-and-apoptosis-inducing-potential-of-hydroalcoholic-achillea-wilhelmsii-c-koch-extract-on-human-breast-adenocarcinoma-cell-lines-mcf-7-and-mda-mb-468
#14
Hamid Reza Galavi, Ramin Saravani, Ali Shahraki, Mojtaba Ashtiani
Achillea wilhelmsii C. Koch contains a variety of components such as flavonoid. The previous studies showed that flavonoid has anti-cancer properties. The aim of the present study was to determine the anti-proliferative and apoptosis-inducing potential of hydroalcoholic Achillea wilhelmsii C. Koch extract (HAWE) on MCF-7 and MDA-Mb-468 human breast carcinoma cell lines. The anti-proliferative activity of HAWE was evaluated using MTT, flowcytometry by annexin V/PI double staining, and caspase-3 activity. The results of MTT showed that the ED50 of MCF-7 and MDA-Mb-468 was 25μg/ml of HAWE, 48h after treatment...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28367423/inhibitory-effect-of-mesenchymal-stem-cell-co-culture-on-erythroid-differentiation-of-k562-cells-compared-to-the-control-group
#15
Mahshid Saleh, Karim Shamsasanjan, Ali Akbari Movassaghpour, Parvin Akbarzadehlaleh, Zahra Molaeipour
OBJECTIVE: Bone marrow mesenchymal stem cells (BMMSCs) reside in the bone marrow and control the process of hematopoiesis. They are an excellent instrument for regenerative treatment and co-culture with hematopoietic stem cells (HSCs). MATERIALS AND METHODS: In this experimental study, K562 cell lines were either treated with butyric acid and co-cultured with MSCs, or cultivated in a conditioned medium from MSCs plus butyric acid for erythroid differentiation. We used the trypan blue dye exclusion assay to determine cell counts and viability in each group...
April 2017: Cell Journal
https://www.readbyqxmd.com/read/28348970/anti-carcinogenic-and-anti-angiogenic-properties-of-the-extracts-of-acorus-calamus-on-gastric-cancer-cells
#16
Samaneh Rahamooz Haghighi, Malek Hossein Asadi, Hassan Akrami, Amin Baghizadeh
OBJECTIVE: Acorus calamus (A. calamus) has been used as a medicinal plant in Asia for its effects on digestive system for the last 2000 years. To investigate the anti-cancer activity of rhizome of A. calamus, the ethanolic and methanolic extracts and essential oil of the rhizome were prepared and their effects were assessed on human gastric cancer cell line (AGS). MATERIALS AND METHODS: The viability of cells which were treated with the extracts and the essential oil was assessed by MTT assay...
March 2017: Avicenna Journal of Phytomedicine
https://www.readbyqxmd.com/read/28341817/impairment-of-macrophage-presenting-ability-and-viability-by-echinococcus-granulosus-antigens
#17
Naceur Mejri, Imed Eddine Hassen, Jenny Knapp, Mouldi Saidi
BACKGROUND: Despite advances toward an improved understanding of the evasive mechanisms leading to the establishment of cystic echinococcosis, the discovery of specific immunosuppressive mechanisms and related factors are of great interest in the development of an immunotherapeutic approach. OBJECTIVE: To elucidate immunosuppressive effects of bioactive factors contained in chromatographic fractions from hydatid cystic fluid (HCF) of Echinococcus granulosus. METHODS: Hydatid cystic fluid was fractionated by reverse phase chromatography...
March 2017: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/28327355/cytotoxic-and-apoptosis-inducing-activity-of-c21-steroids-from-the-roots-of-cynanchum-atratum
#18
Jian Zhang, Lin Ma, Zheng-Feng Wu, Shu-Le Yu, Lei Wang, Wen-Cai Ye, Qing-Wen Zhang, Zhi-Qi Yin
Two new (1-2) and two known C21 steroids (3-4) were isolated from the roots of Cynanchum atratum. Their structures were elucidated by detailed 1D and 2D spectroscopic. The MTT assay showed that compounds 1-4 displayed obvious cytotoxic activities against HepG2 cells with IC50 values ranging from 10.19μM to 76.12μM. Compounds 1-3 also exhibited cytotoxic effects in A549 cells with IC50 values of 30.87-95.39μM. Compound 3 showed the antiproliferative activity via G0/G1 cell cycle arrest and proapoptosis in HepG2 cells by Flowcytometry analysis...
March 19, 2017: Steroids
https://www.readbyqxmd.com/read/28324281/detection-of-minimal-residual-disease-in-childhood-b-acute-lymphoblastic-leukemia-by-4-color-flowcytometry
#19
Ahmad Baraka, Laila M Sherief, Naglaa M Kamal, Shereen El Shorbagy
Monitoring of minimal residual disease (MRD) is currently considered the most powerful predictor of outcome in acute lymphoblastic leukemia (ALL). Achievement of a negative MRD state assessed by multicolor flowcytometry (MFC) is an important predictor of disease-free survival (DFS) and overall survival (OS) in ALL patients. We sought to determine whether panels of antibodies combination are more suitable for detection of MRD in Childhood ALL. Eighty-four (84) patients with ALL (B-lineage subtype) were enrolled in this study...
June 2017: International Journal of Hematology
https://www.readbyqxmd.com/read/28278159/combined-regimen-of-photodynamic-therapy-mediated-by-gallium-phthalocyanine-chloride-and-metformin-enhances-anti-melanoma-efficacy
#20
Diana Tudor, Iuliana Nenu, Gabriela Adriana Filip, Diana Olteanu, Mihai Cenariu, Flaviu Tabaran, Rodica Mariana Ion, Lucian Gligor, Ioana Baldea
BACKGROUND: Melanoma therapy is challenging, especially in advanced cases, due to multiple developed tumor defense mechanisms. Photodynamic therapy (PDT) might represent an adjuvant treatment, because of its bimodal action: tumor destruction and immune system awakening. In this study, a combination of PDT mediated by a metal substituted phthalocyanine-Gallium phthalocyanine chloride (GaPc) and Metformin was used against melanoma. The study aimed to: (1) find the anti-melanoma efficacy of GaPc-PDT, (2) assess possible beneficial effects of Metformin addition to PDT, (3) uncover some of the mechanisms underlining cell killing and anti-angiogenic effects...
2017: PloS One
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