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https://www.readbyqxmd.com/read/28395437/-the-preparation-and-evaluation-of-the-quality-control-materials-for-detection-of-platelet-membrane-glycoproteins-by-flow-cytometry
#1
Y Q Liu, Y Gong, C X Qu, R You, L P Li, L S Xing, J Z Wang
Objective: To prepare the quality control material for detection of platelet membrane glycoproteins by flowcytometry and evaluate the appearance traits, homogeneity and stability of it. Methods: Fresh platelets from the blood group O donors were fixed by the certain concentration of aldehyde solution and then washed by the imidazole buffer. After that, adding certain concentration of lyophilized protection solution into the preparations. The preparations were dispensed to be lyophilized and then were kept refrigerated in 2-8 ℃...
April 11, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28384114/immune-cell-subsets-evaluation-as-a-predictive-tool-for-hepatitis-b-infection-outcome-and-treatment-responsiveness
#2
Snezhina M Kandilarova, Atanaska I Georgieva, Anastasiya P Mihaylova, Marta P Baleva, Valentina K Atanasova, Diana V Petrova, Georgi T Popov, Elissaveta J Naumova
BACKGROUND: The patient's immune response is one of the major factors influencing HBV eradication or chronification, and it is thought to be responsible for the treatment success. AIM: Our study aimed to investigate whether cellular defense mechanisms are associated with the course of HBV infection (spontaneous recovery [SR] or chronification [CHB]) and with the therapeutic approach. PATIENTS AND METHODS: A total of 139 patients (118 with CHB, 21 SR) and 29 healthy individuals (HI) were immunophenotyped by flowcytometry...
March 1, 2017: Folia Medica
https://www.readbyqxmd.com/read/28380412/%C3%AE-asarone-promotes-temozolomide-s-entry-into-glioma-cells-and-decreases-the-expression-of-p-glycoprotein-and-mdr1
#3
Nanbu Wang, Qinxin Zhang, Baile Ning, Laiyu Luo, Yongqi Fang
Glioma is the most common primary brain tumor and has an undesirable prognosis due to the blood-brain barrier (BBB) and drug resistance. A thorough investigation of the changes in intracellular drug concentrations is important to observe therapeutic effects and cell resistance. P-glycoprotein (P-gp) is an essential protein of Multi-drug resistance 1 (MDR1). The over-expression of P-gp and MDR1 is associated with poor prognosis and drug-resistance in glioma. However, β-asarone can pass through the BBB easily and increase the drug concentration in the rat brain...
April 2, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28377223/generation-and-characterization-of-a-human-mouse-chimeric-high-affinity-antibody-that-detects-the-dykddddk-flag-peptide
#4
Koki Ikeda, Tomoaki Koga, Fumiyuki Sasaki, Ayumi Ueno, Kazuko Saeki, Toshiaki Okuno, Takehiko Yokomizo
DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. We recently established a high-affinity monoclonal antibody (mAb) for FLAG (clone 2H8). The 2H8 Ab is highly sensitive for detecting FLAG-tagged proteins by flowcytometry and immunoprecipitation, but it can yield nonspecific signals in immunohistochemistry of mouse tissues because it is of mouse origin. In this study, we reduced nonspecific signals by generating a chimeric 2H8 Ab with Fc fragments derived from human immunoglobulin...
April 1, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28375101/anti-proliferative-and-apoptosis-inducing-potential-of-hydroalcoholic-achillea-wilhelmsii-c-koch-extract-on-human-breast-adenocarcinoma-cell-lines-mcf-7-and-mda-mb-468
#5
Hamid Reza Galavi, Ramin Saravani, Ali Shahraki, Mojtaba Ashtiani
Achillea wilhelmsii C. Koch contains a variety of components such as flavonoid. The previous studies showed that flavonoid has anti-cancer properties. The aim of the present study was to determine the anti-proliferative and apoptosis-inducing potential of hydroalcoholic Achillea wilhelmsii C. Koch extract (HAWE) on MCF-7 and MDA-Mb-468 human breast carcinoma cell lines. The anti-proliferative activity of HAWE was evaluated using MTT, flowcytometry by annexin V/PI double staining, and caspase-3 activity. The results of MTT showed that the ED50 of MCF-7 and MDA-Mb-468 was 25μg/ml of HAWE, 48h after treatment...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28367423/inhibitory-effect-of-mesenchymal-stem-cell-co-culture-on-erythroid-differentiation-of-k562-cells-compared-to-the-control-group
#6
Mahshid Saleh, Karim Shamsasanjan, Ali Akbari Movassaghpour, Parvin Akbarzadehlaleh, Zahra Molaeipour
OBJECTIVE: Bone marrow mesenchymal stem cells (BMMSCs) reside in the bone marrow and control the process of hematopoiesis. They are an excellent instrument for regenerative treatment and co-culture with hematopoietic stem cells (HSCs). MATERIALS AND METHODS: In this experimental study, K562 cell lines were either treated with butyric acid and co-cultured with MSCs, or cultivated in a conditioned medium from MSCs plus butyric acid for erythroid differentiation. We used the trypan blue dye exclusion assay to determine cell counts and viability in each group...
April 2017: Cell Journal
https://www.readbyqxmd.com/read/28348970/anti-carcinogenic-and-anti-angiogenic-properties-of-the-extracts-of-acorus-calamus-on-gastric-cancer-cells
#7
Samaneh Rahamooz Haghighi, Malek Hossein Asadi, Hassan Akrami, Amin Baghizadeh
OBJECTIVE: Acorus calamus (A. calamus) has been used as a medicinal plant in Asia for its effects on digestive system for the last 2000 years. To investigate the anti-cancer activity of rhizome of A. calamus, the ethanolic and methanolic extracts and essential oil of the rhizome were prepared and their effects were assessed on human gastric cancer cell line (AGS). MATERIALS AND METHODS: The viability of cells which were treated with the extracts and the essential oil was assessed by MTT assay...
March 2017: Avicenna Journal of Phytomedicine
https://www.readbyqxmd.com/read/28341817/impairment-of-macrophage-presenting-ability-and-viability-by-echinococcus-granulosus-antigens
#8
Naceur Mejri, Imed Eddine Hassen, Jenny Knapp, Mouldi Saidi
BACKGROUND: Despite advances toward an improved understanding of the evasive mechanisms leading to the establishment of cystic echinococcosis, the discovery of specific immunosuppressive mechanisms and related factors are of great interest in the development of an immunotherapeutic approach. OBJECTIVE: To elucidate immunosuppressive effects of bioactive factors contained in chromatographic fractions from hydatid cystic fluid (HCF) of Echinococcus granulosus. METHODS: Hydatid cystic fluid was fractionated by reverse phase chromatography...
March 2017: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/28327355/cytotoxic-and-apoptosis-inducing-activity-of-c21-steroids-from-the-roots-of-cynanchum-atratum
#9
Jian Zhang, Lin Ma, Zheng-Feng Wu, Shu-Le Yu, Lei Wang, Wen-Cai Ye, Qing-Wen Zhang, Zhi-Qi Yin
Two new (1-2) and two known C21 steroids (3-4) were isolated from the roots of Cynanchum atratum. Their structures were elucidated by detailed 1D and 2D spectroscopic. The MTT assay showed that compounds 1-4 displayed obvious cytotoxic activities against HepG2 cells with IC50 values ranging from 10.19μM to 76.12μM. Compounds 1-3 also exhibited cytotoxic effects in A549 cells with IC50 values of 30.87-95.39μM. Compound 3 showed the antiproliferative activity via G0/G1 cell cycle arrest and proapoptosis in HepG2 cells by Flowcytometry analysis...
March 19, 2017: Steroids
https://www.readbyqxmd.com/read/28324281/detection-of-minimal-residual-disease-in-childhood-b-acute-lymphoblastic-leukemia-by-4-color-flowcytometry
#10
Ahmad Baraka, Laila M Sherief, Naglaa M Kamal, Shereen El Shorbagy
Monitoring of minimal residual disease (MRD) is currently considered the most powerful predictor of outcome in acute lymphoblastic leukemia (ALL). Achievement of a negative MRD state assessed by multicolor flowcytometry (MFC) is an important predictor of disease-free survival (DFS) and overall survival (OS) in ALL patients. We sought to determine whether panels of antibodies combination are more suitable for detection of MRD in Childhood ALL. Eighty-four (84) patients with ALL (B-lineage subtype) were enrolled in this study...
March 21, 2017: International Journal of Hematology
https://www.readbyqxmd.com/read/28278159/combined-regimen-of-photodynamic-therapy-mediated-by-gallium-phthalocyanine-chloride-and-metformin-enhances-anti-melanoma-efficacy
#11
Diana Tudor, Iuliana Nenu, Gabriela Adriana Filip, Diana Olteanu, Mihai Cenariu, Flaviu Tabaran, Rodica Mariana Ion, Lucian Gligor, Ioana Baldea
BACKGROUND: Melanoma therapy is challenging, especially in advanced cases, due to multiple developed tumor defense mechanisms. Photodynamic therapy (PDT) might represent an adjuvant treatment, because of its bimodal action: tumor destruction and immune system awakening. In this study, a combination of PDT mediated by a metal substituted phthalocyanine-Gallium phthalocyanine chloride (GaPc) and Metformin was used against melanoma. The study aimed to: (1) find the anti-melanoma efficacy of GaPc-PDT, (2) assess possible beneficial effects of Metformin addition to PDT, (3) uncover some of the mechanisms underlining cell killing and anti-angiogenic effects...
2017: PloS One
https://www.readbyqxmd.com/read/28215484/establishment-and-characterization-of-a-new-human-acinar-cell-carcinoma-cell-line-faraz-icr-from-pancreas
#12
Marzieh Rezaei, Ahmad Hosseini, Saman Nikeghbalian, Abbas Ghaderi
OBJECTIVES: Basic research in the field of acinar cell carcinoma (ACC) as a rare neoplasm of the pancreas is dependent on the availability of pragmatic model such as new pancreatic cancer cell lines. Thus, establishment and characterization of new pancreatic cancer cell lines from ACC origin are deemed important. METHODS: Faraz-ICR cell line was derived from a 58-years old woman with pancreatic acinar cell carcinoma by the collagenase digestion protocol. We characterized the cell line by examining its morphology and cytostructural and functional profile...
February 8, 2017: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
https://www.readbyqxmd.com/read/28198713/cd1d-restricted-natural-killer-t-cells-are-preserved-in-indian-long-term-non-progressors-ltnps
#13
Dharmendra Singh, Manisha Ghate, Sheela Godbole, Smita Kulkarni, Madhuri Thakar
BACKGROUND: Natural killer T (NKT) cells act as a bridge between innate and adaptive immune responses. Limited information is available regarding the role of NKT cells in the HIV disease progression especially HIV-1 C infection. METHODOLOGY: NKT cells were characterized for their frequency and the activation, aging, exhaustion status and their proliferation ability in 32 long term non progressors (LTNPs), 40 progressors, 18 patients before and after suppressive cART along with 35 HIV-1 negative subjects using multicolor flowcytometry...
February 14, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28195090/mixed-phenotypic-acute-leukemia-series-from-tertiary-care-center
#14
Ravikiran N Pawar, Sambhunath Banerjee, Subhajit Bramha, Shekhar Krishnan, Arpita Bhattacharya, Vaskar Saha, Anupam Chakrapani, Saurabh Bhave, Mammen Chandy, Reena Nair, Mayur Parihar, Neeraj Arora, D K Mishra
INTRODUCTION: Mixed-phenotype acute leukemias (MPALs) are a heterogeneous group of rare leukemias constituting approximately 2%-5% of all leukemias, in which assigning a single lineage of origin is not possible. They are diagnosed by either the presence of antigens of more than one lineage or by the presence of dual population of blasts belonging to two or more lineages. We highlight the clinicopathological, immunophenotype, and genetic data of a cohort (n = 14) of patients diagnosed and treated at our center...
January 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28191813/acute-lymphoblastic-leukemia-mimicking-wilms-tumor-at-presentation
#15
Amitabh Singh, Anirban Mandal, Vijay Guru, Rachna Seth
BACKGROUND: Acute lymphoblastic leukemia (ALL), the commonest malignancy of childhood, is known to manifest with a myriad of atypical presentations. Nephromegaly is a rare presentation of childhood ALL with hepatic mass being even rarer. CASE PRESENTATION: We present a 3 year-old child with unilateral renal mass and hepatic mass lesion with normal blood counts, initially suspected to have metastatic Wilms tumor based on clinical, radiological and WT1 positivity on immunocytochemistry of renal mass...
September 2016: Gulf Journal of Oncology
https://www.readbyqxmd.com/read/28167484/anti-proliferative-and-apoptosis-inducing-potential-of-hydroalcoholic-achillea-wilhelmsii-c-koch-extract-on-human-breast-adenocarcinoma-cell-lines-mcf-7-and-mda-mb-468
#16
Hamid Reza Galavi, Ramin Saravani, Ali Shahraki, Mojtaba Ashtiani
Achillea wilhelmsii C. Koch contains a variety of components such as flavonoid. The previous studies showed that flavonoid has anti-cancer properties. The aim of the present study was to determine the anti-proliferative and apoptosis-inducing potential of hydroalcoholic Achillea wilhelmsii C. Koch extract (HAWE) on MCF-7 and MDA-Mb-468 human breast carcinoma cell lines. The anti-proliferative activity of HAWE was evaluated using MTT, flowcytometry by annexin V/PI double staining, and caspase-3 activity. The results of MTT showed that the ED50 of MCF-7 and MDA-Mb-468 was 25μg/ml of HAWE, 48h after treatment...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28164634/cd38-expression-and-variation-as-a-prognostic-factor-chronic-lymphocytic-leukemia
#17
Mesude Falay, Funda Ceran, Ahmet K Gunes, Simten Dagdas, Meltem Ayli, Gulsum Ozet
BACKGROUND: In this study, we aimed to determine a cutoff level for CD38 that would aid us in identifying chronic lymphocytic leukemia patients in need of early therapy and predicting patients at sufficiently low risk who would likely exhibit a rapid improvement; we also aimed to find out if CD38 expression would show variability during disease course and determine the extent of CD38 expression. METHODS: 124 patients were diagnosed with CLL. CD38 and ZAP-70 expression levels were measured with four color flowcytometry...
July 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28153469/evaluation-of-motor-neuron-differentiation-potential-of-human-umbilical-cord-blood-derived-mesenchymal-stem-cells-in-vitro
#18
Behnam Yousefi, Davood Sanooghi, Faezeh Faghihi, Mohammad Taghi Joghataei, Nourahmad Latifi
Many people suffer from spinal cord injuries annually. These deficits usually threaten the quality of life of patients. As a postpartum medically waste product, human Umbilical Cord Blood (UCB) is a rich source of stem cells with self- renewal properties and neural differentiation capacity which made it useful in regenerative medicine. Since there is no report on potential of human umbilical cord blood-derived mesenchymal stem cells into motor neurons, we set out to evaluate the differentiation properties of these cells into motor neuron-like cells through administration of Retinoic Acid(RA), Sonic Hedgehog(Shh) and BDNF using a three- step in vitro procedure...
January 31, 2017: Journal of Chemical Neuroanatomy
https://www.readbyqxmd.com/read/28153003/protective-effects-of-aucubin-on-osteoarthritic-chondrocyte-model-induced-by-hydrogen-peroxide-and-mechanical-stimulus
#19
In-Chi Young, Sung-Ting Chuang, Chia-Hsien Hsu, Yu-Jun Sun, Hwa-Chang Liu, Yo-Shen Chen, Feng-Huei Lin
BACKGROUND: During the onset of osteoarthritis (OA), certain biochemical events have been shown to accelerate cartilage degradation, including the dysregulation of cartilage ECM anabolism, abnormal generation of reactive oxygen species (ROS) and overproduction of proteolytic enzymes and inflammatory cytokines. The potency of aucubin in protecting cellular components against oxidative stress, inflammation and apoptosis effects are well documented, which makes it a potential candidate for OA treatment...
February 2, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28104464/genetic-defects-in-pi3k%C3%AE-affect-b-cell-differentiation-and-maturation-leading-to-hypogammaglobulineamia-and-recurrent-infections
#20
Marjolein Wentink, Virgil Dalm, Arjan C Lankester, Pauline A van Schouwenburg, Liesbeth Schölvinck, Tomas Kalina, Radana Zachova, Anna Sediva, Annechien Lambeck, Ingrid Pico-Knijnenburg, Jacques J M van Dongen, Malgorzata Pac, Ewa Bernatowska, Martin van Hagen, Gertjan Driessen, Mirjam van der Burg
BACKGROUND: Mutations in PIK3CD and PIK3R1 cause activated PI3K-δ syndrome (APDS) by dysregulation of the PI3K-AKT pathway. METHODS: We studied precursor and peripheral B-cell differentiation and apoptosis via flowcytometry. Furthermore, we performed AKT-phosphorylation assays and somatic hypermutations (SHM) and class switch recombination (CSR) analysis. RESULTS: We identified 13 patients of whom 3 had new mutations in PIK3CD or PIK3R1. Patients had low total B-cell numbers with increased frequencies of transitional B cells and plasmablasts, while the precursor B-cell compartment in bone marrow was relatively normal...
January 17, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
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