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Flowcytometry

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https://www.readbyqxmd.com/read/29781883/cytomegalovirus-specific-cd4-t-cell-responses-and-cmv-igg-levels-are-associated-with-neurocognitive-impairment-in-people-living-with-hiv
#1
Susanne Dam Nielsen, Vibe Ballegaard, Karin Kaereby Pedersen, Maria Pedersen, Peter Brændstrup, Nikolai Kirkby, Anette Stryhn Buus, Lars P Ryder, Jan Gerstoft
BACKGROUND: Mechanisms leading to neurocognitive impairment (NCI) in people living with HIV (PLWHIV) on stable antiretroviral therapy (cART) remain unknown. We investigated the association between CMV immunity, HIV-specific variables and NCI in PLWHIV on stable cART and with low comorbidity. METHODS: 52 PLWHIV on stable cART and 31 HIV-uninfected controls matched on age, gender, education and comorbidity were tested with a neurocognitive test-battery and CMV-immunoglobulin G (CMV-IgG) levels were measured...
May 16, 2018: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/29752081/effect-of-polyhydroxybutyrate-chitosan-bioglass-nanofiber-scaffold-on-proliferation-and-differentiation-of-stem-cells-from-human-exfoliated-deciduous-teeth-into-odontoblast-like-cells
#2
Maryam Khoroushi, Mohammad Reza Foroughi, Saeed Karbasi, Batool Hashemibeni, Abbas Ali Khademi
Scaffolds and their characteristics play a central role in tissue engineering. The purpose of this study was to determine the effects of Polyhydroxybutyrate (PHB)/Chitosan/nano-bioglass (nBG) nanofiber scaffold made using the electrospinning method, on the proliferation and differentiation of stem cells obtained from human exfoliated deciduous teeth into odontoblast-like cells. In this experimental study, the pulps of the molten deciduous teeth were isolated, thereafter, the stem cells from human exfoliated deciduous teeth (SHED) were extracted and then the 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cell viability percentage...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29741994/development-of-a-novel-explant-culture-method-for-the-isolation-of-mesenchymal-stem-cells-from-human-breast-tumor
#3
Koushan Sineh Sepehr, Alireza Razavi, Mohsen Saeidi, Majid Mossahebi-Mohammadi, Meghdad Abdollahpour-Alitappeh, Seyed Mahmoud Hashemi
BACKGROUND: Mesenchymal stem cells (MSCs) were isolated from various sources, including various types of tumors. However choosing an appropriate isolation method is an important step in obtaining cells with optimal quality and yield in companion with economical considerations. The purpose of this study was to isolate of more pure MSCs from human breast tumor tissue by a modified explant culture method. METHODS AND MATERIALS: The tumor tissues (n = 8) was cut into 1 to 3-mm cube-like pieces (explant)...
May 9, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29728233/multifunctional-chitosan-capped-gold-nanoparticles-for-enhanced-cancer-chemo-radiotherapy-an-invitro-study
#4
Mohamed Mahmoud Fathy, Fayrouz S Mohamed, NihalSaad Elbialy, Wael M Elshemey
Over the last decade, chemo-radiotherapy represented a well-established paradigm for cancer treatment. Developing new strategies to promote the therapeutic efficacy while reducing toxic side effects of chemo-radiotherapy is a main research objective in cancer therapy. A promising new oncological strategy for enhancing chemo-radiotherapy against cancer involves the utilization of multifunctional nanoparticles (nanocarriers and radiosensitizers). In this work, Chitosan-Capped Gold Nanoparticles (CS-GNPs) were synthesized and loaded with an anticancer agent, Doxorubicin (CS-GNPs-DOX)...
April 2018: Physica Medica: PM
https://www.readbyqxmd.com/read/29710378/placental-expression-of-asialoglycoprotein-receptor-associated-with-hepatitis-b-virus-transmission-from-mother-to-child
#5
Ashish Kumar Vyas, Usha Ramakrishna, Bijoya Sen, Mojahidul Islam, Gayatri Ramakrishna, Sharda Patra, Archana Rastogi, Shiv Kumar Sarin, Nirupma Trehanpati
BACKGROUND: Asialoglycoprotein receptor (ASGPR) expression on hepatocytes has been associated with endocytosis, binding and uptake of HBV. The role of ASGPR in HBV vertical transmission and its expression on placenta has not yet been studied. PATIENTS AND METHODS: Thirty four HBsAg+ve and thirteen healthy pregnant mothers along with their newborns were enrolled. The former were categorized into transmitting and non-transmitting mothers based on their newborns being HBsAg and HBV DNA positive...
April 30, 2018: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/29704020/role-of-chicoric-acid-and-13-cis-retinoic-acid-in-mycobacterium-tuberculosis-infection-control-by-human-u937-macrophage
#6
Bahareh Abd-Nikfarjam, Marjan Nassiri-Asl, Mehri Hajiaghayi, Taghi Naserpour Farivar
Mycobacterium tuberculosis (Mtb) survives and proliferates within the main cells of the innate immune system, macrophages. The goal of our study was to investigate the immunostimulatory effects of 13-cis retinoic acid (RA) and chicoric acid (CA) in human U937 macrophages against H37Ra Mtb infection by evaluating its potential role in the cell surface expression of HLA-DR, CD14 molecules as well as nitric oxide (NO) production and prevention of the Mtb growth within macrophages. In this study, we investigated the effects of 13-cis RA and CA on Mtb-infected macrophages using flowcytometry and Griess methods, respectively...
April 27, 2018: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/29689714/production-and-characterization-of-anti-human-igg-f-ab-2-antibody-fragment
#7
Zahra Valedkarimi, Hadi Nasiri, Leili Aghebati-Maleki, Jalal Abdolalizadeh, Mojghan Esparvarinha, Jafar Majidi
In present study an optimized protocol for the separation of antibodies into antigen-binding fragments F(ab')2 using pepsin digestion was investigated. The production of these fragments is a consequential step in the development of medical research, treatment and diagnosis. For production of polyclonal antibody rabbit received antigen in four steps. The rabbit serum at 1/128000 dilution showed high absorbance in reaction with human IgG at the designed ELISA method. Rabbit IgG was purified by Ion-Exchange Chromatography (IEC) method...
April 10, 2018: Human Antibodies
https://www.readbyqxmd.com/read/29673738/tbk1-a-key-regulator-and-potential-treatment-target-for-interferon-positive-sj%C3%A3-gren-s-syndrome-systemic-lupus-erythematosus-and-systemic-sclerosis
#8
Iris L A Bodewes, Erika Huijser, Cornelia G van Helden-Meeuwsen, Liselotte Tas, Ruth Huizinga, Virgil A S H Dalm, P Martin van Hagen, Noortje Groot, Sylvia Kamphuis, Paul L A van Daele, Marjan A Versnel
OBJECTIVE: Upregulation of type I interferons (IFN-I) is a hallmark of systemic autoimmune diseases like primary Sjögren's syndrome (pSS), systemic lupus erythematosus (SLE) and systemic sclerosis (SSc). Expression of IFN-I is induced by three different receptor families: Toll-like receptors (TLRs), RIG-like receptors (RLRs) and DNA-sensing receptors (DSRs). TANK-binding kinase (TBK1) is an important signaling hub downstream of RLRs and DSRs. TBK1 activates IRF3 and IRF7, leading to IFN-I production and subsequent induction of interferon stimulated genes (ISGs)...
April 16, 2018: Journal of Autoimmunity
https://www.readbyqxmd.com/read/29627514/combined-effects-of-photobiomodulation-and-alendronate-on-viability-of-osteoporotic-bone-marrow-derived-mesenchymal-stem-cells
#9
Somaye Fallahnezhad, Abdollah Amini, Behnam Hajihossainlou, Sufan Chien, Sara Dadras, Fatemehalsadat Rezaei, Mohammad Bayat
Osteoporotic bone marrow mesenchymal stem cells (BMMSCs) are involved in the pathogenesis of osteoporosis (OP). Photobiomodulation (PBM) has positive effects on healthy BMMSCs. The goal of current experiment was to evaluate the combined influence of photobiomodulation PBM and alendronate (ALN) incubation on ovariectomized induced osteoporosis(OVX)- BMMSC viability in vitro. 15 female adult Wistar rats were distributed into the 2 groups: (1) 3 healthy (sham)control rats, (2) 12 OVX- rats. All OVX rats underwent ovariectomy...
March 22, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29622866/the-inhibitory-effect-of-epigallocatechin-gallate-on-the-viability-of-t-lymphoblastic-leukemia-cells-is-associated-with-increase-of-caspase-3-level-and-fas-expression
#10
Masome Ghasemi-Pirbaluti, Batoul Pourgheysari, Hedayatollah Shirzad, Zahra Sourani, Pezhman Beshkar
Acute lymphoblastic leukemia is the most prevalent cancer in children. Novel components to help struggle aggressive malignancies and overcome some side effects of conventional treatments could be a promising strategy. Epigallocatechingallate (EGCG), have attracted the attention of scientists for prevention or treatment of some cancers. Jurkat cells were incubated with the different concentrations of EGCG (30-100 µm) for 24, 48, and 72 h and cell viability was investigated using MTS test. Apoptosis and the level of caspase 3 alterations were evaluated using flowcytometry and expression of Fas by Real Time PCR...
April 2018: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/29622864/comparison-of-immunohistochemistry-cytochemistry-and-flow-cytometry-in-aml-for-myeloperoxidase-detection
#11
Ankur Ahuja, Seema Tyagi, Tulika Seth, Hara Prasad Pati, Gps Gahlot, Preeti Tripathi, Venkatesan Somasundaram, Renu Saxena
Acute Myeloid Leukemia (AML) as per World Health Organization (WHO 2008) classification is on the basis of the antigenic characterization, enzymes restriction in the neoplastic myeloid cells and the specific translocations/mutations. AML can be assessed and differentiated by flowcytometry (FCM)/immunohistochemistry (IHC)/cytochemistry techniques. Myeloperoxidase (MPO) is an unequivocal marker to differentiate AML from the acute lymphoblastic leukemia. Despite FCM popularity, it has its limitations, in form of 'dry-tap', cost, and inability of being performed by retrospective analysis...
April 2018: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/29609005/decreased-pd-1-expression-on-circulating-cd4-t-cell-and-pd-l1-expression-on-myeloid-dendritic-cells-correlate-with-clinical-manifestations-in-systemic-juvenile-idiopathic-arthritis
#12
Li Cai, Chenxing Zhang, Jing Wu, Wei Zhou, Tongxin Chen
OBJECTIVES: Programmed cell death-1 (PD-1) and its ligand (PD-L1) mediate negative signal in autoimmune diseases. While little is known about its role in juvenile idiopathic arthritis (JIA). The study aimed to reveal the circulating cell profile and the relative PD-1/PD-L1 expression of JIA subsets, elucidating their underlying immunomodulatory mechanisms. METHODS: We detected the circulating cells and the relative PD-1/PD-L1 signaling in 101 JIA patients and 50 controls by flowcytometry and analyzed their association with disease activity and clinical manifestations...
March 30, 2018: Joint, Bone, Spine: Revue du Rhumatisme
https://www.readbyqxmd.com/read/29589548/characterization-of-regulatory-t-cells-in-multiple-sclerosis-patients-treated-with-interferon-beta-1a
#13
Mohsen Ebrahimi, Ali Ganji, Sarah Zahedi, Parisa Nourbakhsh, Keyvan Ghasami, Ghasem Mosayebi
BACKGROUND: Regulatory T-Cells (Treg Cells), as one of the immune system components, have been highly effective in the autoimmune diseases prevention, particularly multiple sclerosis (MS). Cytokine-based therapies such as interferon beta-1a (IFN-β1a) is a common drug in MS treatment; however, its exact mechanisms are insufficiently described. OBJECTIVE: Therefore, the goal of this study was to evaluate the in vivo impact of IFN-β1a on the Treg Cells in MS. METHODS: In this case-control study, Treg Cells were analysed by flowcytometry in IFN-β1a-treated relapsing-remitting MS (RRMS) in comparison with new cases of MS and healthy subjects...
March 27, 2018: CNS & Neurological Disorders Drug Targets
https://www.readbyqxmd.com/read/29587544/ferula-pseudalliacea-induces-apoptosis-in-human-colorectal-cancer-hct-116-cells-via-mitochondria-dependent-pathway
#14
Hadi Bamehr, Massoud Saidijam, Dara Dastan, Razieh Amini, Mona Pourjafar, Rezvan Najafi
Ferula species have diverse biological functions. This study set out to investigate the anti-proliferative effects of methanolic extract of F. pseudalliacea against human colon cancer HCT-116 cell line. Cytotoxic effects of F. pseudalliacea on HCT-116 cells was estimated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. Real-time polymerase chain reaction (PCR) and Western blot were employed to analyze BAX and Bcl2 expression. Cell cycle analysis and apoptosis were conducted using flowcytometry and Annexin V/ propidium iodide (PI) staining...
March 27, 2018: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/29576778/cytotoxicity-assessment-of-polyhydroxybutyrate-chitosan-nano-bioglass-nanofiber-scaffolds-by-stem-cells-from-human-exfoliated-deciduous-teeth-stem-cells-from-dental-pulp-of-exfoliated-deciduous-tooth
#15
Batool Hashemi-Beni, Maryam Khoroushi, Mohammad Reza Foroughi, Saeed Karbasi, Abbas Ali Khademi
Background: The aim of this study was to compare the cytotoxicity and the biocompatibility of three different nanofibers scaffolds after seeding of stem cells harvested from human deciduous dental pulp. Given the importance of scaffold and its features in tissue engineering, this study demonstrated the construction of polyhydroxybutyrate (PHB)/chitosan/nano-bioglass (nBG) nanocomposite scaffold using electrospinning method. Materials and Methods: This experimental study was conducted on normal exfoliated deciduous incisors obtained from 6-year-old to 11-year-old healthy children...
March 2018: Dental Research Journal
https://www.readbyqxmd.com/read/29544524/defective-formyl-peptide-receptor-2-3-and-annexin-a1-expressions-associated-with-m2a-polarization-of-blood-immune-cells-in-patients-with-chronic-obstructive-pulmonary-disease
#16
Yung-Che Chen, Meng-Chih Lin, Chih-Hung Lee, Shih-Feng Liu, Chin-Chou Wang, Wen-Feng Fang, Tung-Ying Chao, Chao-Chien Wu, Yu-Feng Wei, Huang-Chih Chang, Chia-Cheng Tsen, Hung-Chen Chen
BACKGROUND: Controversy exists in previous studies on macrophage M1/M2 polarization in chronic obstructive pulmonary disease (COPD). We hypothesized that formyl peptide receptor (FPR), a marker of efferocytosis and mediator of M1/M2 polarization, may be involved in the development of COPD. METHODS: We examined FPR 1/2/3 expressions of blood M1/M2a monocyte, neutrophil, natural killer (NK) cell, NK T cell, T helper (Th) cell, and T cytotoxic (Tc) cell by flowcytometry method in 40 patients with cigarette smoking-related COPD and 16 healthy non-smokers...
March 15, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29505809/age-associated-distribution-of-normal-b-cell-and-plasma-cell-subsets-in-peripheral-blood
#17
Elena Blanco, Martín Pérez-Andrés, Sonia Arriba-Méndez, Teresa Contreras-Sanfeliciano, Ignacio Criado, Ondrej Pelak, Ana Serra-Caetano, Alfonso Romero, Noemí Puig, Ana Remesal, Juan Torres Canizales, Eduardo López-Granados, Tomas Kalina, Ana E Sousa, Menno van Zelm, Mirjam van der Burg, Jacques J M van Dongen, Alberto Orfao
BACKGROUND: Humoral immunocompetence develops stepwise throughout life and contributes to individual susceptibility to infection, immunodeficiency, autoimmunity, and neoplasia. Immunoglobulin heavy chain (IgH) isotype serum levels can partly explain such age-related differences, but their relationship with the IgH isotype distribution within memory B-cell (MBC) and plasma cell (PCs) compartments remains to be investigated. OBJECTIVE: We studied the age-related distribution of MBCs and PCs expressing different IgH isotypes in addition to the immature/transitional and naive B-cell compartments...
March 2, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29482257/histone-deacetylase-inhibitor-trapoxin-a-enhances-stemness-properties-in-adipose-tissue-derived-mesenchymal-stem-cells
#18
Leila Mousazadeh, Effat Alizadeh, Nosratollah Zarghami, Shahryar Hashemzadeh, Sedigheh Fekri Aval, Leili Hasanifard, Roya Herizchi
BACK GROUND: Adipose tissue derived mesenchymal stem cells (ASCs) have unique potential for regenerative cell therapies. However, during ex-vivo cultivation, they undergo considerable quality loss regarding their phenotypic properties, stemness genes expression and differentiation potential. Recent studies reported that the loss of stemness properties of MSCs is a result of chromatin histone deacetylations through in-vitro cultivation. The present work aimed to study the effect of Trapoxin A (TPX) as a histone deacetylase inhibitor (HDACi) on overall stemness properties of ASCs...
February 26, 2018: Drug Research
https://www.readbyqxmd.com/read/29456809/hypericin-photodynamic-therapy-inhibits-proliferation-and-induces-apoptosis-in-human-rheumatoid-arthritis-fibroblast-like-synoviocytes-cell-line-mh7a
#19
Kun Zhang, Shan Gao, JiaYi Guo, GuoHua Ni, Zhe Chen, Feng Li, XiaoLei Zhu, YongBing Wen, YanXing Guo
Objectives: To elucidate the effects and potential mechanisms of hypericin-photodynamic therapy (HYP-PDT) for treating the human rheumatoid arthritis (RA) fibroblast-like synoviocyte (FLS) MH7A cell-line. Materials and Methods: MH7A cells were subjected to HYP-PDT intervention and apoptosis was evaluated via MTT, nuclear staining, and flowcytometry analyses. Intracellular reactive oxygen species (ROS) were measured with the fluorescent probe 2'7'-dichlorofluorescein diacetate (DCFH-DA)...
February 2018: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29453555/evaluation-of-the-effect-of-hyperthermia-and-electron-radiation-on-prostate-cancer-stem-cells
#20
Zhila Rajaee, Samideh Khoei, Seied Rabi Mahdavi, Marzieh Ebrahimi, Sakine Shirvalilou, Alireza Mahdavian
The aim of this study was to investigate the effect of hyperthermia, 6 MeV electron radiation and combination of these treatments on cancer cell line DU145 in both monolayer culture and spheroids enriched for prostate cancer stem cells (CSCs). Flowcytometric analysis of the expression of molecular markers CD133+ /CD44+ was carried out to determine the prostate CSCs in cell line DU145 grown as spheroids in serum-free medium. Following monolayer and spheroid culture, DU145 cells were treated with different doses of hyperthermia, electron beam and combination of them...
May 2018: Radiation and Environmental Biophysics
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