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https://www.readbyqxmd.com/read/27910003/bone-marrow-t-cell-percentage-a-novel-prognostic-indicator-in-acute-myeloid-leukemia
#1
Manar M Ismail, Nahla A B Abdulateef
Acute myeloid leukemia (AML) is an aggressive malignancy for which overall disease-free survival is less than 50%. Manipulation of the immune system is an interesting and promising therapy for AML patients. We aimed to characterize the immune system of AML patients, highlighting the clinical relevance of total bone marrow (BM) lymphocytes and subpopulations. Sixty-six new AML cases diagnosed according to WHO criteria from King Abdullah Medical City, KSA, from October 2012 to February 2015. Analysis of BM lymphocytes and subpopulations was done by flowcytometry...
December 1, 2016: International Journal of Hematology
https://www.readbyqxmd.com/read/27895854/differential-expression-of-aristaless-like-homeobox-4-a-potential-marker-for-gastric-adenocarcinoma
#2
Fariba Ghasemvand, Navid Nezafat, Saeed Hesami Tackallou, Daruosh Momenzadeh, Saeid Rahmanzadeh
AIM: The objective of this experiment was to evaluate the ALX-4 mRNA expression level in different stages of human gastric adenocarcinoma compared to the gastric cancer stem cells (GCSC) and gastric cancer cell line, MKN-45. BACKGROUND: Gastric cancer is the second most common cancer in the world today, leading approximately to 3-10% of all cancer-related deaths. Identification of specific biomarkers could be a crucial approach to improve diagnosis and treatment of this cancer type...
2016: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/27872702/gamma-reactivation-using-the-spongy-effect-of-klf1-binding-site-sequence-an-approach-in-gene-therapy-for-beta-thalassemia
#3
Nasrin Heydari, Laleh Shariati, Hossein Khanahmad, Zahra Hejazi, Mansoureh Shahbazi, Mansoor Salehi
OBJECTIVES: β-thalassemia is one of the most common genetic disorders in the world. As one of the promising treatment strategies, fetal hemoglobin (Hb F) can be induced. The present study was an attempt to reactivate the γ-globin gene by introducing a gene construct containing KLF1 binding sites to the K562 cell line. MATERIALS AND METHODS: A plasmid containing a 192 bp sequence with two repeats of KLF1 binding sites on β-globin and BCL11A promoters was constructed and used to transfect the K562 cell line...
October 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27834108/flow-cytometric-white-blood-differential-using-cytodiff%C3%A2-in-the-diagnosis-of-neonatal-early-onset-infection
#4
R van Vliet, H K van den Tooren-de Groot, Ap van Rossum
OBJECTIVE: Neonatal early-onset infection is a life-threatening disease, requiring early diagnosis and treatment. Newborns at risk are identified by a combination of risk factors, clinical signs of infection and laboratory parameters such as white blood cell count and C-reactive protein (CRP). This method is labor-intensive, time consuming and has a variable reproducibility. New reliable diagnostic markers are needed to identify neonatal infection. This study presents additional leukocyte differential parameters produced by the automated flowcytometry and processing software using CytoDiff™ reagent (Beckman Coulter) in newborns suspected for early-onset infection...
November 11, 2016: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/27805454/ferulic-acid-fa-abrogates-ionizing-radiation-induced-oxidative-damage-in-murine-spleen
#5
Ujjal Das, Sushobhan Biswas, Aaveri Sengupta, Krishnendu Manna, Anindita Chakraborty, Sanjit Dey
PURPOSE: Spleen is a crucial organ manifesting immune functions. Thus, radiation induced oxidative challenge is vulnerable for spleen. Our major objective was to protect the spleen from radiation induced anomalous situations and to identify the signaling pathways involved. MATERIALS AND METHODS: Swiss albino mice were treated with ferulic acid (FA) once in a day at a dose of 50 mg/kg body weight for 5 consecutive days before exposing them to single dose of 10 Gy irradiation...
November 2, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27796140/in-vitro-and-in-vivo-evaluation-of-paclitaxel-lapatinib-loaded-f127-pluronic-micelles
#6
Pouya Dehghankelishadi, Ebrahim Saadat, Fatemeh Ravar, Maliheh Safavi, Mahboobeh Pordeli, Mehdi Gholami, Farid A Dorkoosh
The aim of this study was to evaluate the in vitro and in vivo efficacy of Paclitaxel-Lapatinib loaded Pluronic micelles. Lapatinib and pluronic sensitize the cancerous cells to Paclitaxel via efflux pump inhibition. In addition, pluronic polymers can trigger intrinsic apoptosis pathways. Furthermore, micellar system can passively target the chemotherapeutic agents by enhanced permeability and retention effect. The paclitaxel-lapatinib loaded micelles were characterized in means of encapsulation efficacy and size...
October 31, 2016: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/27782868/evidence-of-il-17-producing-innate-lymphoid-cells-in-peripheral-blood-from-patients-with-enteropathic-spondyloarthritis
#7
Paola Triggianese, Paola Conigliaro, Maria Sole Chimenti, Livia Biancone, Giovanni Monteleone, Roberto Perricone, Ivan Monteleone
OBJECTIVES: Both the innate and the adaptive immune responses contribute to the onset of chronic inflammation in spondyloarthritis (SpA). The association between SpA and inflammatory bowel disease (IBD, enteropathic SpA-ESpA) has been largely established and suggests a shared pathophysiology. There is evidence that innate lymphoid cells (ILC) are involved in the pathogenesis of both SpA and IBD while no evidence has been reported to date on ESpA. We aimed to analyse for the first time the frequency and cytokine expression of ILC in peripheral blood from ESpA patients compared with both IBD and healthy subjects...
October 21, 2016: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/27766226/nf-k%C3%AE-activation-in-u266-cells-on-mesenchymal-stem-cells
#8
Sara Zahedi, Karim Shamsasenjan, Aliakbar Movassaghpour, Parvin Akbarzadehlaleh
Purpose: Mesenchymal Stem Cells (MSCs) are one of the essential members of Bone Marrow (BM) microenvironment and the cells affect normal and malignant cells in BM milieu. One of the most important hematological malignancies is Multiple Myeloma (MM). Numerous studies reported various effects of MSCs on myeloma cells. MSCs initiate various signaling pathways in myeloma cells, particularly NF-kβ. NF-kβ signaling pathway plays pivotal role in the survival, proliferation and resistance of myeloma cells to the anticancer drugs, therefore this pathway can be said to be a vital target for cancer therapy...
September 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27760639/-study-on-the-survival-rate-of-random-flap-using-pre-injection-of-adscs
#9
B S Wang, H Y Yang, Y Han
Objective: To determine the effects of adipose-derived stem cells (ADSCs) on the survival rate of flaps by transplanting ADSCs into the experimental flaps pre-operatively. Methods: ADSCs were isolated from fresh human fat and cultured in vitro. Their morphological characters were observed, and flowcytometry and differentiation test and evaluation were conducted. A total of 40 BALB/c mice were divided into 5 groups randomly with each of 8 mice. 1 cm×4 cm random skin flap was designed on the mice back, ADSCs were injected in advance into subcutaneous tissues of 3 groups, the operation was performed on the 2(nd,) 5(th) and 7(th) day after the ADSCs injection, meanwhile skin flap operation was conducted in the immediate injection group and control group...
September 27, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/27755958/sirna-dox-lodeded-chitosan-based-nanoparticles-development-characterization-and-in-vitro-evaluation-on-a549-lung-cancer-cell-line
#10
M Seifi-Najmi, M Hajivalili, R Safaralizadeh, S Sadreddini, S Esmaeili, R Razavi, M Ahmadi, H Mikaeili, B Baradaran, K Shams-Asenjan, M Yousefi
High-mobility group AT-hook2 (HMGA2), involved in epithelial mesenchymal transition (EMT) process, has a pivotal role in lung cancer metastasis. Lung cancer therapy with HMGA2 suppressing small interfering RNA (siRNA) has been introduced recently while doxorubicin (DOX) has been used as a frequent cancer chemotherapy agent. Both reagents have been faced with obstacles in clinic which make them ineffective. NanoParticles (NPs) provided a platform for efficient co delivery of the anticancer drugs. The aim of this study was production and in vitro characterization of different pharmacological groups (siRNA, DOX or siRNA-DOX) of carboxymethyl dextran thrimethyl chitosan nanoparticles (CMDTMChiNPs) on cytotoxicity, gene expression, apoptosis and migration of metastatic lung cancer cell line (A-549)...
September 30, 2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/27751956/characterization-of-culture-filtrate-proteins-rv1197-and-rv1198-of-esat-6-family-from-mycobacterium-tuberculosis-h37rv
#11
Himanshu Pandey, Sarita Tripathi, Kanchan Srivastava, Dinesh K Tripathi, Mrigank Srivastava, Surya Kant, Kishore K Srivastava, Ashish Arora
BACKGROUND: We have characterized two immunogenic proteins, Rv1197 and Rv1198, of the Esx-5 system of the ESAT-6 family of Mycobacterium tuberculosis H37Rv. METHODS: The complex formation between Rv1197 and Rv1198 was characterized by biophysical techniques. The reactivity of serum from TB patients towards these proteins was characterized by ELISA. Lymphocyte proliferation and cytokine induction were followed in restimulated splenocytes from immunized mice by using MTT assay and CBA flowcytometry, respectively...
October 15, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27748272/high-fms-like-tyrosine-kinase-3-flt3-receptor-surface-expression-predicts-poor-outcome-in-flt3-internal-tandem-duplication-itd-negative-patients-in-adult-acute-myeloid-leukaemia-a-prospective-pilot-study-from-india
#12
Surender Kumar Sharawat, Vinod Raina, Lalit Kumar, Atul Sharma, Radhika Bakhshi, Sreenivas Vishnubhatla, Ritu Gupta, Sameer Bakhshi
BACKGROUND & OBJECTIVES: Mutations in fms-like tyrosine kinase 3 (FLT3) receptor have significant role in assessing outcome in patients with acute myeloid leukaemia (AML). Data for FLT3 surface expression in relation to FLT3 internal tandem duplication (ITD) status and outcome are not available from India. The objective of the current study was to investigate adult patients with AML for FLT3 expression and FLT3 ITD mutation, and their association with long-term outcome. METHODS: Total 51 consecutive de novo AML patients aged 18-60 yr were enrolled in the study...
May 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/27746865/evaluation-of-interleukin17and-interleukin-23-expression-in-patients-with-active-and-latent-tuberculosis-infection
#13
Fatemeh Heidarnezhad, Amir Asnaashari, Seyed Abdolrahim Rezaee, Roghayeh Ghezelsofla, Kiarash Ghazvini, Narges Valizadeh, Reza Basiri, Aghigh Ziaeemehr, Somayeh Sobhani, Houshang Rafatpanah
OBJECTIVES: Tuberculosis is one of the most important infectious diseases with high mortality rates worldwide, especially in developing countries. Interleukin17 (IL-17) is an important acquired immunity cytokine, which is mainly produced by CD4(+)TH17 cells. It can recruit neutrophils and macrophages to the infected site in the lungs. IL-23 is one of the most important inducers of IL-17. In the present study, the expressions of IL-23 and IL-17 were examined in the pathogenesis of tuberculosis...
August 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27712979/novel-polyacrylate-based-cationic-nanoparticles-for-survivin-sirna-delivery-combined-with-mitoxantrone-for-treatment-of-breast-cancer
#14
Sanam Arami, Majid Mahdavi, Mohammad Reza Rashidi, Marziyeh Fathi, Mohammad-Saeid Hejazi, Nasser Samadi
As a gene delivery method in breast cancer therapy, knocking down the undesired genes in the cancerous cells would be promising. Inhibitors of Apoptosis Protein (IAP) family genes are some of the genes whose responsibility is inhibition of apoptosis in cells. Silencing these genes seems to be helpful directing the tumor cells to death. siRNA sequence designed against survivin anti-apoptotic gene can play this role if carried to the cytoplasm. Here we prepared a positive charged biocompatible nano-sized particle made up of a Fe3O4 core covered respectively by polyacrylate (PA) and polyethyleneimine (PEI) layer, which could successfully deliver the siRNA into the MCF-7 cells...
October 3, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27702507/fabrication-of-aptamer-decorated-dextran-coated-nano-graphene-oxide-for-targeted-drug-delivery
#15
Mona Alibolandi, Marzieh Mohammadi, Seyed Mohammad Taghdisi, Mohammad Ramezani, Khalil Abnous
In the current study, dextran (DEX) was covalently conjugated to the surface of nano-GO sheets, making stable biocompatible dextran coated GO (GO-DEX). The prepared GO-DEX was nontoxic to 4T1 mammary carcinoma cell line at concentrations up to 300μg/mL. AS1411 aptamer, a ssDNA aptamer which can improve the intracellular uptake by nucleolin recognition, also has been introduced to hydroxyl groups of DEX in GO-DEX to produce GO-DEX-Apt. Moreover, curcumin (CUR), a natural polyphenol, found in the rhizomes of Curcuma longa (turmeric) which shows antineoplastic effects, was loaded onto the GO-DEX and GO-DEX-Apt via π-π stacking interactions with a high loading capacity (∼29wt%)...
January 2, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27697451/does-being-overweight-affect-seminal-variables-in-fertile-men
#16
Emad A Taha, Sohair K Sayed, Hisham D Gaber, Hatem K Abdel Hafez, Nagwa Ghandour, Asmaa Zahran, Taymour Mostafa
The effect of being overweight on seminal variables was assesed in 165 fertile men. Participants were divided into three groups: fertile men with normal body mass index (BMI) (18.5-24.9 kg/m(2)), fertile overweight men (BMI 25-29.9 kg/m(2)) and fertile obese men (BMI >30 kg/m(2)). Medical history was taken, a clinical examination conducted. Semen analysis was undertaken and BMI measured. Seminal reactive oxygen species (ROS) was estimated by chemiluminescent assay, sperm vitality by the hypo-osmotic swelling test and sperm DNA fragmentation by propidium iodide staining with flowcytometry...
September 16, 2016: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/27671509/natural-killer-cell-cytotoxicity-against-skov3-after-hla-g-downregulation-by-shrna
#17
Nazanin Nazari, Shirin Farjadian
BACKGROUND: HLA-G is a nonclassical HLA class I molecule which, when elevated in tumor cells, is one of the main factors involved in tumor evasion of immune responses including NK and T cells. OBJECTIVE: To evaluate the effect of HLA-G downregulation on NK cell cytotoxicity in tumor cell lines. METHODS: The expression level of HLA-G was measured by real-time PCR and flowcytometry after transfection of SKOV3 by shRNA.1, which targets specific sequences in exon 4, or shRNA...
September 2016: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/27669541/sucrose-but-not-glucose-blocks-il1-%C3%AE-induced-inflammatory-response-in-human-chondrocytes-by-inducing-autophagy-via-akt-mtor-pathway
#18
Nazir M Khan, Mohammad Y Ansari, Tariq M Haqqi
Pathogenesis of osteoarthritis (OA) is multifactorial but interleukin-1β (IL-1β) is known to be an important mediator of cartilage degradation. Autophagy is an essential cellular homeostasis mechanism and has been proposed to protect against cartilage degradation and chondrocyte death under pathological conditions. We investigated the role of autophagy activated by sucrose, a natural disaccharide, in suppressing inflammatory mediator's expression and cell death under pathological conditions in human chondrocytes...
September 26, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27664934/impaired-akt-phosphorylation-in-b-cells-of-patients-with-common-variable-immunodeficiency
#19
Reza Yazdani, Mazdak Ganjalikhani-Hakemi, Mohammad Esmaeili, Hassan Abolhassani, Shahram Vaeli, Abbas Rezaei, Zohre Sharifi, Gholamreza Azizi, Nima Rezaei, Asghar Aghamohammadi
Common variable immunodeficiency (CVID) is a heterogeneous group of primary immunodeficiency characterized by recurrent infectious. We evaluated whether defective PI3K/Akt/FoxO pathway could influence B-cell fate. Determination of B-cell subsets in CVD patients and healthy donors (HDs) were performed using flow cytometry. We evaluated mRNA level and protein expression of PI3K, Akt and FoxO molecules using real-time PCR and flow cytometry, respectively. Moreover, phosphorylated Akt (p-Akt) expression in B-cells has also been measured by flowcytometry...
September 21, 2016: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/27635203/effects-of-valproic-acid-and-pioglitazone-on-cell-cycle-progression-and-proliferation-of-t-cell-acute-lymphoblastic-leukemia-jurkat-cells
#20
Marie Saghaeian Jazi, Saeed Mohammadi, Yaghoub Yazdani, Sima Sedighi, Ali Memarian, Mehrdad Aghaei
OBJECTIVES: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematologic malignant tumor. Administration of chemical compounds influencing apoptosis and T cell development has been discussed as promising novel therapeutic strategies. Valproic acid (VPA) as a recently emerged anti-neoplastic histone deacetylase (HDAC) inhibitor and pioglitazone (PGZ) as a high-affinity peroxisome proliferator-activated receptor-gamma (PPARγ) agonist have been shown to induce apoptosis and cell cycle arrest in different studies...
July 2016: Iranian Journal of Basic Medical Sciences
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