María Alcaide, Anatoli Tchigvintsev, Mónica Martínez-Martínez, Ana Popovic, Oleg N Reva, Álvaro Lafraya, Rafael Bargiela, Taras Y Nechitaylo, Ruth Matesanz, Marie-Anne Cambon-Bonavita, Mohamed Jebbar, Michail M Yakimov, Alexei Savchenko, Olga V Golyshina, Alexander F Yakunin, Peter N Golyshin, Manuel Ferrer
The shrimp Rimicaris exoculata dominates the fauna in deep-sea hydrothermal vent sites along the Mid-Atlantic Ridge (depth, 2,320 m). Here, we identified and biochemically characterized three carboxyl esterases from microbial communities inhabiting the R. exoculata gill that were isolated by naive screens of a gill chamber metagenomic library. These proteins exhibit low to moderate identity to known esterase sequences (≤52%) and to each other (11.9 to 63.7%) and appear to have originated from unknown species or from genera of Proteobacteria related to Thiothrix/Leucothrix (MGS-RG1/RG2) and to the Rhodobacteraceae group (MGS-RG3)...
March 2015: Applied and Environmental Microbiology