keyword
MENU ▼
Read by QxMD icon Read
search

Rnd

keyword
https://www.readbyqxmd.com/read/29764369/vareporter-variant-reporter-for-cancer-research-of-massive-parallel-sequencing
#1
Po-Jung Huang, Chi-Ching Lee, Ling-Ya Chiu, Kuo-Yang Huang, Yuan-Ming Yeh, Chia-Yu Yang, Cheng-Hsun Chiu, Petrus Tang
BACKGROUND: High throughput sequencing technologies have been an increasingly critical aspect of precision medicine owing to a better identification of disease targets, which contributes to improved health care cost and clinical outcomes. In particular, disease-oriented targeted enrichment sequencing is becoming a widely-accepted application for diagnostic purposes, which can interrogate known diagnostic variants as well as identify novel biomarkers from panels of entire human coding exome or disease-associated genes...
May 9, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29760777/a-pseudomonas-putida-efflux-pump-acts-on-short-chain-alcohols
#2
Georg Basler, Mitchell Thompson, Danielle Tullman-Ercek, Jay Keasling
Background: The microbial production of biofuels is complicated by a tradeoff between yield and toxicity of many fuels. Efflux pumps enable bacteria to tolerate toxic substances by their removal from the cells while bypassing the periplasm. Their use for the microbial production of biofuels can help to improve cell survival, product recovery, and productivity. However, no native efflux pump is known to act on the class of short-chain alcohols, important next-generation biofuels, and it was considered unlikely that such an efflux pump exists...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29740005/nitrothiophene-carboxamides-a-novel-narrow-spectrum-antibacterial-series-mechanism-of-action-and-efficacy
#3
Shahul Hameed P, Nagakumar Bharatham, Nainesh Katagihallimath, Sreevalli Sharma, Radha Nandishaiah, Anirudh P Shanbhag, Teby Thomas, Riya Narjari, Maitrayee Sarma, Purnendu Bhowmik, Prakruthi Amar, Rajani Ravishankar, Ramesh Jayaraman, Kubendran Muthan, Ramesh Subbiah, Vasanthi Ramachandran, V Balasubramanian, Santanu Datta
The mechanism of efflux is a tour-de-force in the bacterial armoury that has thwarted the development of novel antibiotics. We report the discovery of a novel chemical series with potent antibacterial properties that was engineered to overcome efflux liability. Compounds liable to efflux specifically via the Resistance Nodulation and cell Division (RND) pump, AcrAB-TolC were chosen for a hit to lead progression. Using structure-based design, the compounds were optimised to lose their binding to the efflux pump, thereby making them potent on wild-type bacteria...
May 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29739333/renal-denervation-improves-cardiac-function-by-attenuating-myocardiocyte-apoptosis-in-dogs-after-myocardial-infarction
#4
Li Wang, Lijun Song, Chao Li, Qiaoli Feng, Mengping Xu, Zhuqing Li, Chengzhi Lu
BACKGROUND: Myocardial apoptosis is important in the pathogenesis and progression of myocardial infarction-induced heart failure (MI-HF). Renal sympathetic denervation (RDN) has become a promising therapeutic strategy for the treatment of HF. Previous studies have shown that RDN could improve heart function Yao et al. (Exp Ther Med 14:4104-4110, 2017). However, whether and how RDN regulates myocardial apoptosis in MI-HF is unclear. This study sought to evaluate the effects of RDN on cardiac function and apoptosis-related gene expression in MI-HF dogs...
May 8, 2018: BMC Cardiovascular Disorders
https://www.readbyqxmd.com/read/29725253/anti-biofilm-and-anti-virulence-efficacy-of-celastrol-against-stenotrophomonas-maltophilia
#5
Hye-Rim Kim, Dongsup Lee, Yong-Bin Eom
Stenotrophomonas maltophilia is a multi-drug resistant opportunistic pathogen that causes nosocomial infections in immunocompromised patients. This pathogen is difficult to treat owing to its intrinsic multidrug resistance and ability to form antimicrobial-tolerant biofilms. In the present study, we aimed to assess the potential use of celastrol as a novel anti-biofilm and/or anti-virulence agent against S. maltophilia . Results showed that celastrol at its sub-inhibitory doses decreased biofilm formation and disrupt the established biofilms produced by S...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29697028/bacterial-multidrug-efflux-proteins-a-major-mechanism-of-antimicrobial-resistance
#6
Irshad Ahmad, Nighat Nawaz, Fatemeh Karimi Dermani, Alisa Khodadadi Kohlan, Massoud Saidijam, Simon G Patching
Antimicrobial resistance is a global challenge, for which the mechanisms of resistance are varied and complicated. Bacterial multidrug efflux proteins are a major mechanism of antimicrobial resistance and therefore targets for drug development. Gram-negative bacteria are intrinsically more resistant to many antibiotics and biocides due to their cell structure and the activity of multidrug efflux proteins. These transporters actively extrude antibiotics and other xenobiotics from the cytoplasm or surrounding membranes of cells to the external environment...
April 25, 2018: Current Drug Targets
https://www.readbyqxmd.com/read/29687766/identification-and-prevalence-of-rnd-family-multidrug-efflux-pump-oqxab-genes-in-enterococci-isolates-from-swine-manure-in-china
#7
Li Yuan, Ya-Jun Zhai, Hua Wu, Hua-Run Sun, Zhi-Pei He, Ya-Bin Wang, Yu-Shan Pan, Nan-Nan Kuang, Gong-Zheng Hu
PURPOSE: The resistance/nodulation/cell division (RND) family multidrug efflux pump, OqxAB, has been identified as one of the leading mechanisms of plasmid-mediated quinolone resistance and has become increasingly prevalent among Enterobacteriaceae in recent years. However, oqxAB genes have not yet been reported in Enterococcus isolates. The aim of the present study was to identify the oqxAB genes and investigate their prevalence among Enterococcus from swine manure in China. METHODOLOGY: The oqxAB genes were screened in 87 Enterococcus isolates by PCR...
April 23, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29661860/substrate-specificities-and-efflux-efficiencies-of-rnd-efflux-pumps-of-acinetobacter-baumannii
#8
Inga V Leus, Jon W Weeks, Vincent Bonifay, Lauren Smith, Sophie Richardson, Helen I Zgurskaya
Antibiotic resistant Acinetobacter baumannii causes infections that are extremely difficult to treat. A significant role in these resistance profiles is attributed to multidrug efflux pumps, especially those belonging to Resistance-Nodulation-cell Division (RND) superfamily of transporters. In this study, we analyzed functions and properties of RND efflux pumps in A. baumannii ATCC 17978. This strain is susceptible to antibiotics and does not contain mutations that are commonly selected upon exposure to high concentrations of antibiotics...
April 16, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29625653/the-role-of-efflux-pumps-in-bacteroides-fragilis-resistance-to-antibiotics
#9
REVIEW
Reza Ghotaslou, Mina Yekani, Mohammad Yousef Memar
The resistance of Bacteroides fragilis to the most antimicrobial agents has been reported in the world. Identification of the microbial resistance mechanisms can play an important role in controlling these resistances. Currently, B. fragilis is resistant to most antibiotics. The multi-drug efflux pumps have been shown to underlie the antimicrobial resistance in B. fragilis strains. Two types of these efflux pumps including RND and MATE can be regarded as main structures responsible for antibiotic resistance...
May 2018: Microbiological Research
https://www.readbyqxmd.com/read/29577985/rnd-transporters-in-the-living-world
#10
Hiroshi Nikaido
Transporters of the RND superfamily are well-known as the major drug efflux pumps of Gram-negative bacteria. However, they are widespread in organisms ranging from Archaea to Eukaryotes, and perform diverse functions. This review gives a brief overview of these diverse members of the superfamily with emphasis on their structure and functions.
March 22, 2018: Research in Microbiology
https://www.readbyqxmd.com/read/29562911/application-of-next-generation-sequencing-to-characterize-novel-mutations-in-clarithromycin-susceptible-helicobacter-pylori-strains-with-a2143g-of-23s-rrna-gene
#11
Jiaoe Chen, Liping Ye, Liangmin Jin, Xuehua Xu, Peisong Xu, Xianjun Wang, Hongzhang Li
BACKGROUND: Clarithromycin (CLR) resistance has become a predominant factor for treatment failure of Helicobacter pylori eradication. Although the molecular mechanism of CLR resistance has been clearly understood in H. pylori, it is lack of evidence of other genes involved in drug resistance. Furthermore, the molecular mechanism of phenotype susceptible to CLR while genotype of 23S rRNA is mutant with A2143G is unclear. Here, we characterized the mutations of CLR-resistant and -susceptible H...
March 22, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29554339/diversity-of-mutations-in-regulatory-genes-of-resistance-nodulation-cell-division-efflux-pumps-in-association-with-tigecycline-resistance-in-acinetobacter-baumannii
#12
Stefanie Gerson, Jennifer Nowak, Esther Zander, Julia Ertel, Yurong Wen, Oleg Krut, Harald Seifert, Paul G Higgins
Objectives: To investigate the mechanisms of tigecycline resistance in isogenic Acinetobacter baumannii isolate pairs as well as 65 unique clinical A. baumannii isolates obtained during the MagicBullet clinical trial from Greece, Italy and Spain. Methods: A. baumannii isolates were subjected to WGS and the regulatory genes of resistance-nodulation-cell division (RND)-type efflux pumps were analysed. MICs were determined by agar dilution and the expression of RND-type efflux pumps was measured by semi-quantitative RT-PCR...
March 14, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29548026/the-consistent-differential-expression-of-genetic-pathways-following-exposure-of-an-industrial-pseudomonas-aeruginosa-strain-to-preservatives-and-a-laundry-detergent-formulation
#13
Angharad E Green, Alejandro Amézquita, Yvan Le-Marc, Matthew J Bull, Thomas R Connor, Eshwar Mahenthiralingam
Pseudomonas aeruginosa (P. aeruginosa) is a common contaminant associated with product recalls in the home and personal care (HPC) industry. Preservation systems are used to prevent spoilage and protect consumers, but greater knowledge is needed of preservative resistance mechanisms used by P. aeruginosa contaminants. We aimed to identify genetic pathways associated with preservative exposure by using an industrial P. aeruginosa strain and implementing RNA-Seq to understand gene expression changes in response to industry relevant conditions...
March 14, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29506266/a-putative-rnd-type-efflux-pump-h239_3064-contributes-to-colistin-resistance-through-crrb-in-klebsiella-pneumoniae
#14
Yi-Hsiang Cheng, Tzu-Lung Lin, Yi-Tsung Lin, Jin-Town Wang
Background: Colistin is one of the last-resort antibiotics used to treat carbapenem-resistant Klebsiella pneumoniae infection. Our previous studies indicated that clinical strains encoding CrrB with amino acid substitutions exhibited higher colistin resistance (MICs ≥512 mg/L) than did colistin-resistant strains encoding mutant MgrB, PmrB or PhoQ. Objectives: CrrAB may regulate another unknown mechanism(s) contributing to colistin resistance, besides modifications of LPS with 4-amino-4-deoxy-l-arabinose and phosphoethanolamine...
February 28, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29491882/a-comprehensive-atlas-of-e3-ubiquitin-ligase-mutations-in-neurological-disorders
#15
REVIEW
Arlene J George, Yarely C Hoffiz, Antoinette J Charles, Ying Zhu, Angela M Mabb
Protein ubiquitination is a posttranslational modification that plays an integral part in mediating diverse cellular functions. The process of protein ubiquitination requires an enzymatic cascade that consists of a ubiquitin activating enzyme (E1), ubiquitin conjugating enzyme (E2) and an E3 ubiquitin ligase (E3). There are an estimated 600-700 E3 ligase genes representing ~5% of the human genome. Not surprisingly, mutations in E3 ligase genes have been observed in multiple neurological conditions. We constructed a comprehensive atlas of disrupted E3 ligase genes in common (CND) and rare neurological diseases (RND)...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29448259/applicability-of-computerized-planimetric-method-in-estimating-plaque-accumulation-and-efficacy-of-a-cleaning-method-for-removable-dental-prostheses
#16
Ahmad Al Jaghsi, Reiner Biffar, Christiane Pink, Torsten Mundt
PURPOSE: To demonstrate how data collected using the computerized planimetric method (CPM) can be analyzed by providing an example in which the amount of plaque accumulation and the efficacy of the rotating needles device (RND) in cleaning specific parts of partial removable dental prostheses (PRDPs) were estimated. MATERIALS AND METHODS: Thirty-four participants wearing a total of 41 double-crown-retained PRDPs with veneered secondary crowns were included in this study...
February 15, 2018: International Journal of Prosthodontics
https://www.readbyqxmd.com/read/29434938/genetic-characterization-and-in-vitro-activity-of-antimicrobial-combinations-of-multidrug-resistant-acinetobacter-baumannii-from-a-general-hospital-in-china
#17
Fang Chen, Ling Wang, Min Wang, Yixin Xie, Xiaomeng Xia, Xianping Li, Yanhua Liu, Wei Cao, Tingting Zhang, Pengling Li, Min Yang
The present study aimed to develop a rational therapy based on the genetic epidemiology, molecular mechanism evaluation and in vitro antibiotic combinations activity in multidrug-resistant Acinetobacter baumannii (MDRAB). MDRAB was screened by the Kirby-Bauer method. The random amplified polymorphic DNA technique was used to establish genetic fingerprinting, and a series of resistance genes were detected by polymerase chain reaction. Antimicrobial agents including amikacin (AK), cefoperazone/sulbactam (SCF I/II), meropenem (MEM), minocycline (MINO) and ciprofloxacin (CIP) were used to determine the minimum inhibitory concentrations (MICs) and interactions between antibiotics by the broth microdilution method and chequerboard assays...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29424299/medicinal-chemistry-updates-on-bacterial-efflux-pump-modulators
#18
Fernando Andre Pereira Marques Duraes, Madalena Maria Magalhaes Pinto, Maria Emilia Silva Pereira de Sousa
Antibiotic resistance is one of the most pressing health issues of our days. It can arise due to a multiplicity of factors, such as target modification, decrease in the drug uptake, changes in the metabolic pathways and activation of efflux pumps. The overexpression of efflux pumps is responsible for the extrusion of drugs, making antibiotic therapy fail, as the quantity of intracellular antibiotic is not enough to provide the desired therapeutic effect. Efflux pumps can be included in five families according to their composition, nature of substrates, energy source, and number of transmembrane spanning regions...
February 9, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29407044/computer-simulations-of-the-activity-of-rnd-efflux-pumps
#19
Attilio Vittorio Vargiu, Venkata Krishnan Ramaswamy, Giuliano Malloci, Ivana Malvacio, Alessio Atzori, Paolo Ruggerone
The putative mechanism by which bacterial RND-type multidrug efflux pumps recognize and transport their substrates is a complex and fascinating enigma of structural biology. How a single protein can recognize a huge number of unrelated compounds and transport them through one or just a few mechanisms is an amazing feature not yet completely unveiled. The appearance of cooperativity further complicates the understanding of structure-dynamics-activity relationships in these complex machineries. Experimental techniques may have limited access to the molecular determinants and to the energetics of key processes regulating the activity of these pumps...
January 31, 2018: Research in Microbiology
https://www.readbyqxmd.com/read/29398525/remote-coupled-drastic-%C3%AE-barrel-to-%C3%AE-sheet-transition-of-the-protein-translocation-motor
#20
Arata Furukawa, Shintaro Nakayama, Kunihito Yoshikaie, Yoshiki Tanaka, Tomoya Tsukazaki
The membrane protein SecDF, belonging to the RND superfamily, enhances protein translocation at the extracytoplasmic side using a proton gradient. Here, we report the crystal structure of SecDF in a form we named Super-membrane-facing (Super F) form, demonstrating a β-barrel architecture instead of the previously reported β-sheet structure. Through this structural insight and supporting results of an in vivo crosslinking experiment, we propose a remote coupling model in which a structural change of the transmembrane region drives a functional, extracytoplasmic conformational transition...
March 6, 2018: Structure
keyword
keyword
62264
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"