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https://www.readbyqxmd.com/read/28631419/the-cpxr-cpxa-system-contributes-to-salmonella-gold-resistance-by-controlling-the-gols-dependent-gesabc-transcription
#1
Sebastián Cerminati, Germán F Giri, Julián I Mendoza, Fernando C Soncini, Susana K Checa
Several regulatory systems contribute to bacterial resistance to heavy metals controlling the expression of factors required to eliminate the intoxicant and/or to repair the damage caused by it. In Salmonella, the response to Au ions is mediated by the specific metalloregulator GolS that, among other genes, controls the expression of the RND-efflux pump GesABC. In this work, we demonstrate that CpxR/CpxA, a main cell-envelope stress-responding system, promotes gesABC transcription in the presence of Au ions at neutral pH...
June 19, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28584156/the-role-of-axyz-transcriptional-regulator-in-the-overproduction-of-axyxy-oprz-multidrug-efflux-system-in-achromobacter-spp-mutants-selected-by-tobramycin
#2
Julien Bador, Catherine Neuwirth, Nadège Grangier, Marie Muniz, Leslie Germé, Jérémy Bonnet, Vignesh-Guru Pillay, Catherine Llanes, Claire de Curraize, Lucie Amoureux
AxyXY-OprZ is an RND-type efflux system that confers innate aminoglycoside resistance to Achromobacter spp. We investigated here about a putative TetR family transcriptional regulator encoded by the axyZ gene located upstream of axyXY-oprZ In-frame axyZ gene deletion assay led to increased MICs of antibiotic substrates of the efflux system: aminoglycosides, cefepime, fluoroquinolones, tetracyclines and erythromycin, indicating that the product of axyZ negatively regulates expression of axyXY-oprZ Moreover we identified an amino-acid substitution at position 29 of AxyZ (V29G) in a clinical Achromobacter strain that occurred during the course of chronic respiratory tract colonization in a cystic fibrosis (CF) patient...
June 5, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28538019/if-background-lung-abnormalities-do-not-affect-the-presentation-of-spontaneous-pneumothorax-is-lung-resection-always-justified
#3
Rocco Bilancia, Annabel J Sharkey, Amit Paik, David A Waller
BACKGROUND: The suggestion that spontaneous pneumothorax (SP) may result from diffuse porosity rather than discrete anatomic abnormality challenges the practice of targeted bullectomy. We assessed whether underlying pulmonary abnormalities are correlated or could be predicted from the mode of presentation, with potential implications for treatment. MATERIALS AND METHODS: We analyzed 192 consecutive video-assisted thoracoscopic surgery resections for SP (139 primary, 53 secondary) in 171 patients (115 male, age 36, range, 16 to 81)...
May 19, 2017: Journal of Bronchology & Interventional Pulmonology
https://www.readbyqxmd.com/read/28536672/response-to-bile-salts-in-clinical-strains-of-acinetobacter-baumannii-lacking-the-adeabc-efflux-pump-virulence-associated-with-quorum-sensing
#4
Maria López, Lucia Blasco, Eva Gato, Astrid Perez, Laura Fernández-Garcia, Luis Martínez-Martinez, Felipe Fernández-Cuenca, Jesús Rodríguez-Baño, Alvaro Pascual, German Bou, Maria Tomás
Introduction:Acinetobacter baumannii is an opportunistic nosocomial pathogen associated with multiple infections. This pathogen usually colonizes (first stage of microbial infection) host tissues that are in contact with the external environment. As one of the sites of entry in human hosts is the gastrointestinal tract, the pathogen must be capable of tolerating bile salts. However, studies analyzing the molecular characteristics involved in the response to bile salts in clinical strains of A. baumannii are scarce...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28522287/patterns-of-regional-lymphadenectomy-for-clinically-node-negative-patients-with-penile-carcinoma-analysis-from-the-national-cancer-database-from-1998-to-2012
#5
Juan Chipollini, Dominic H Tang, Pranav Sharma, Adam S Baumgarten, Philippe E Spiess
PURPOSE: Evidence supports upfront regional lymphadenectomy (rND) when primary penile tumors exhibit high-risk features and negative inguinal adenopathy (cN0). We sought to analyze trends in the utilization of early rND as well as assess factors associated with its use and survival outcomes using a nationwide cancer registry database. PATIENT AND METHODS: The National Cancer Database was queried for patients with clinically nonmetastatic penile carcinoma and available nodal status who underwent rND from 1998 to 2012...
April 26, 2017: Clinical Genitourinary Cancer
https://www.readbyqxmd.com/read/28512090/the-vibrio-cholerae-vexgh-rnd-efflux-system-maintains-cellular-homeostasis-by-effluxing-vibriobactin
#6
Dillon E Kunkle, X Renee Bina, James E Bina
Resistance-nodulation-division (RND) superfamily efflux systems have been widely studied for their role in antibiotic resistance, but their native biological functions remain poorly understood. We previously showed that loss of RND-mediated efflux in Vibrio cholerae resulted in activation of the Cpx two-component regulatory system, which mediates adaptation to stress resulting from misfolded membrane proteins. Here, we investigated the mechanism linking RND-mediated efflux to the Cpx response. We performed transposon mutagenesis screening of RND-deficient V...
May 16, 2017: MBio
https://www.readbyqxmd.com/read/28462872/interaction-of-ethidium-and-tetraphenylphosphonium-cations-with-salmonella-enterica-cells
#7
Valeryia Mikalayeva, Sandra Sakalauskaitė, Rimantas Daugelavičius
BACKGROUND AND OBJECTIVE: One of the main causes of bacterial resistance to antimicrobials is multidrug resistance induced by the increased efficiency of the efflux pumps. In this study we analyzed how the conditions of assay affect the efflux of indicator substrates ethidium (Et(+)) and tetraphenylphosphonium (TPP(+)) in Salmonella enterica ser. Typhimurium cells. Impact of the outer membrane permeability barrier, composition and temperature of the medium on accumulation of the indicator compounds also was analyzed...
April 17, 2017: Medicina
https://www.readbyqxmd.com/read/28352264/indole-induced-activities-of-%C3%AE-lactamase-and-efflux-pump-confer-ampicillin-resistance-in-pseudomonas-putida-kt2440
#8
Jisun Kim, Bora Shin, Chulwoo Park, Woojun Park
Indole, which is widespread in microbial communities, has received attention because of its effects on bacterial physiology. Pseudomonas putida and Pseudomonas aeruginosa can acquire ampicillin (Amp) resistance during growth on indole-Amp agar. Transcriptome, mutant, and inhibitor studies have suggested that Amp resistance induced by indole can be attributed to increased gene expression of ttgAB encoding two genes of RND-type multidrug efflux operons and an ampC encoding β-lactamase. Expression, enzyme activities, and mutational analyses indicated that AmpC β-lactamase is important for acquiring Amp resistance of P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28340510/the-diverse-family-of-mmpl-transporters-in-mycobacteria-from-regulation-to-antimicrobial-developments
#9
REVIEW
Albertus Viljoen, Violaine Dubois, Fabienne Girard-Misguich, Mickaël Blaise, Jean-Louis Herrmann, Laurent Kremer
Mycobacterial genomes contain large sets of loci encoding membrane proteins that belong to a family of multidrug resistance pumps designated Resistance-Nodulation-Cell Division (RND) permeases. Mycobacterial membrane protein Large (MmpL) transporters represent a subclass of RND transporters known to participate in the export of lipid components across the cell envelope. These surface-exposed lipids with unusual structures play key roles in the physiology of mycobacteria and/or can act as virulence factors and immunomodulators...
June 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28314727/transcriptomic-analyses-elucidate-adaptive-differences-of-closely-related-strains-of-pseudomonas-aeruginosa-in-fuel
#10
Thusitha S Gunasekera, Loryn L Bowen, Carol E Zhou, Susan C Howard-Byerly, William S Foley, Richard C Striebich, Larry C Dugan, Oscar N Ruiz
Pseudomonas aeruginosa can utilize hydrocarbons, but different strains have various degrees of adaptation despite their highly conserved genome. P. aeruginosa ATCC 33988 is highly adapted to hydrocarbons, while P. aeruginosa strain PAO1, a human pathogen, is less adapted and degrades jet fuel at a lower rate than does ATCC 33988. We investigated fuel-specific transcriptomic differences between these strains in order to ascertain the underlying mechanisms utilized by the adapted strain to proliferate in fuel...
May 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28284638/does-antifouling-paint-select-for-antibiotic-resistance
#11
Carl-Fredrik Flach, Chandan Pal, Carl Johan Svensson, Erik Kristiansson, Marcus Östman, Johan Bengtsson-Palme, Mats Tysklind, D G Joakim Larsson
There is concern that heavy metals and biocides contribute to the development of antibiotic resistance via co-selection. Most antifouling paints contain high amounts of such substances, which risks turning painted ship hulls into highly mobile refuges and breeding grounds for antibiotic-resistant bacteria. The objectives of this study were to start investigate if heavy-metal based antifouling paints can pose a risk for co-selection of antibiotic-resistant bacteria and, if so, identify the underlying genetic basis...
March 8, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28275586/colistin-resistance-in-acinetobacter-baumannii-mdr-zj06-revealed-by-a-multiomics-approach
#12
Xiaoting Hua, Lilin Liu, Youhong Fang, Qiucheng Shi, Xi Li, Qiong Chen, Keren Shi, Yan Jiang, Hua Zhou, Yunsong Yu
Acinetobacter baumannii has emerged as an important opportunistic pathogen due to its ability to acquire resistance to most currently available antibiotics. Colistin is often considered as the last line of therapy for infections caused by multidrug-resistant A. baumannii (MDRAB). However, colistin-resistant A. baumannii strain has recently been reported. To explore how multiple drug-resistant A. baumannii responded to colistin resistance, we compared the genomic, transcriptional and proteomic profile of A. baumannii MDR-ZJ06 to the induced colistin-resistant strain ZJ06-200P5-1...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28230771/antibiotic-resistance-determinant-focused-acinetobacter-baumannii-vaccine-designed-using-reverse-vaccinology
#13
Zhaohui Ni, Yan Chen, Edison Ong, Yongqun He
As one of the most influential and troublesome human pathogens, Acinetobacter baumannii (A. baumannii) has emerged with many multidrug-resistant strains. After collecting 33 complete A. baumannii genomes and 84 representative antibiotic resistance determinants, we used the Vaxign reverse vaccinology approach to predict classical type vaccine candidates against A. baumannii infections and new type vaccine candidates against antibiotic resistance. Our genome analysis identified 35 outer membrane or extracellular adhesins that are conserved among all 33 genomes, have no human protein homology, and have less than 2 transmembrane helices...
February 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28210464/evaluation-of-efflux-pumps-gene-expression-in-resistant-pseudomonas-aeruginosa-isolates-in-an-iranian-referral-hospital
#14
Babak Pourakbari, Sahar Yaslianifard, Somaye Yaslianifard, Shima Mahmoudi, Sepideh Keshavarz-Valian, Setareh Mamishi
BACKGROUND AND OBJECTIVES: Pseudomonas aeruginosa (PA) is one of the most important causes of nosocomial infections and has an intrinsic resistance to many antibiotics. Among all the resistance-nodulation-division (RND) pumps of P. aeruginosa, MexAB-OprM is the first efflux pump found to target multiple classes of antibiotics. This study was aimed to evaluate the expression level of genes expressing MexAB-OprM in clinical isolates of P. aeruginosa. MATERIALS AND METHODS: In this study, 45 P...
August 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28207789/carbapenem-resistance-and-pathogenicity-of-bovine-acinetobacter-indicus-like-isolates
#15
Peter Klotz, Stephan Göttig, Ursula Leidner, Torsten Semmler, Sandra Scheufen, Christa Ewers
The objective of this study was to characterize blaOXA-23 harbouring Acinetobacter indicus-like strains from cattle including genomic and phylogenetic analyses, antimicrobial susceptibility testing and evaluation of pathogenicity in vitro and in vivo. Nasal and rectal swabs (n = 45) from cattle in Germany were screened for carbapenem-non-susceptible Acinetobacter spp. Thereby, two carbapenem resistant Acinetobacter spp. from the nasal cavities of two calves could be isolated. MALDI-TOF mass spectrometry and 16S rDNA sequencing identified these isolates as A...
2017: PloS One
https://www.readbyqxmd.com/read/28064102/influence-of-components-of-tumour-microenvironment-on-the-response-of-hct-116-colorectal-cancer-to-the-ruthenium-based-drug-nami-a
#16
Alberta Bergamo, Chiara Pelillo, Angela Chambery, Gianni Sava
Solid tumours are constituted of tumour cells, healthy cells recruited from the host tissues and soluble factors released by both these cell types. The present investigation examines the capacity of co-cultures between the HCEC colon epithelial cells and the HCT-116 colorectal cancer cells (mimicking the primary site of tumour growth) and between IHH hepatocytes and the HCT-116 colorectal cancer cells (metastatic site) to influence the effects of NAMI-A (imidazolium trans-imidazoledimethylsulphoxidetetrachloro ruthenate) on the tumour cells themselves...
December 2, 2016: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28044273/rnd-efflux-pump-mediated-antibiotic-resistance-in-gram-negative-bacteria-escherichia-coli-and-pseudomonas-aeruginosa-a-major-issue-worldwide
#17
REVIEW
Minakshi Puzari, Pankaj Chetia
Therapeutic failures against diseases due to resistant Gram-negative bacteria have become a major threat nowadays as confirmed by surveillance reports across the world. One of the methods of development of multidrug resistance in Escherichia coli and Pseudomonas aeruginosa is by means of RND efflux pumps. Inhibition of these pumps might help to combat the antibiotic resistance problem, for which the structure and regulation of the pumps have to be known. Moreover, judicious antibiotic use is needed to control the situation...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28034459/calcium-induces-tobramycin-resistance-in-pseudomonas-aeruginosa-by-regulating-rnd-efflux-pumps
#18
Sharmily Khanam, Manita Guragain, Dirk L Lenaburg, Ryan Kubat, Marianna A Patrauchan
Pseudomonas aeruginosa is an opportunistic multidrug resistant pathogen causing severe chronic infections. Our previous studies showed that elevated calcium (Ca(2+)) enhances production of several virulence factors and plant infectivity of the pathogen. Here we show that Ca(2+) increases resistance of P. aeruginosa PAO1 to tobramycin, antibiotic commonly used to treat Pseudomonas infections. LC-MS/MS-based comparative analysis of the membrane proteomes of P aeruginosa grown at elevated versus not added Ca(2+), determined that the abundances of two RND (resistance-nodulation-cell division) efflux pumps, MexAB-OprM and MexVW-OprM, were increased in the presence of elevated Ca(2+)...
January 2017: Cell Calcium
https://www.readbyqxmd.com/read/27956321/prevalence-incidence-and-risk-factors-for-shoulder-and-neck-dysfunction-after-neck-dissection-a-systematic-review
#19
REVIEW
E M Gane, Z A Michaleff, M A Cottrell, S M McPhail, A L Hatton, B J Panizza, S P O'Leary
INTRODUCTION: Shoulder pain and dysfunction may occur following neck dissection among people being treated for head and neck cancer. This systematic review aims to examine the prevalence and incidence of shoulder and neck dysfunction after neck dissection and identify risk factors for these post-operative complications. METHODS: Electronic databases (Pubmed, CINAHL, EMBASE, Cochrane) were searched for articles including adults undergoing neck dissection for head and neck cancer...
November 17, 2016: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/27932117/risk-factors-of-recurrence-of-diabetic-nephropathy-in-renal-transplants
#20
B Rodriguez Cubillo, B Rodriguez, M Calvo, V de la Manzanara, J Bautista, I Perez-Flores, N Calvo, A Moreno, A Shabaka, J Delgado, A I Sanchez-Fructuoso
BACKGROUND: Renal transplantation has been established as the treatment of choice for end-stage renal disease (ESRD) due to diabetic nephropathy. This study aimed to investigate the risk factors for recurrence of diabetic nephropathy (RDN) in renal allografts. METHODS: We studied 1,011 renal transplant patients from 1986 to 2003, of which 95 had ESRD due to diabetic nephropathy. We retrospectively analyzed the clinical characteristics and outcomes of RDN after renal transplantation...
November 2016: Transplantation Proceedings
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