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https://www.readbyqxmd.com/read/26824408/the-effect-of-limiting-the-range-of-perimetric-sensitivities-on-pointwise-assessment-of-visual-field-progression-in-glaucoma
#1
Stuart K Gardiner, William H Swanson, Shaban Demirel
PURPOSE: Automated perimetry does not produce reliable estimates of true psychophysical threshold in glaucomatous visual fields when the perimetric threshold falls below 15 to 19 dB. It may be possible to truncate testing at such locations and not use stimuli with very high contrast. However, this can only be recommended if it does not harm the ability to monitor change. This study examined the effect of applying such a cutoff by censoring sensitivities in two existing longitudinal datasets...
January 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/19544035/sequential-peptide-affinity-purification-system-for-the-systematic-isolation-and-identification-of-protein-complexes-from-escherichia-coli
#2
Mohan Babu, Gareth Butland, Oxana Pogoutse, Joyce Li, Jack F Greenblatt, Andrew Emili
Biochemical purification of affinity-tagged proteins in combination with mass spectrometry methods is increasingly seen as a cornerstone of systems biology, as it allows for the systematic genome-scale characterization of macromolecular protein complexes, representing demarcated sets of stably interacting protein partners. Accurate and sensitive identification of both the specific and shared polypeptide components of distinct complexes requires purification to near homogeneity. To this end, a sequential peptide affinity (SPA) purification system was developed to enable the rapid and efficient isolation of native Escherichia coli protein complexes (J Proteome Res 3:463-468, 2004)...
2009: Methods in Molecular Biology
https://www.readbyqxmd.com/read/16777091/human-sgt-interacts-with-bag-6-bat-3-scythe-and-cells-with-reduced-levels-of-either-protein-display-persistence-of-few-misaligned-chromosomes-and-mitotic-arrest
#3
Marc Winnefeld, Annabel Grewenig, Martina Schnölzer, Herbert Spring, Tobias A Knoch, Eugene C Gan, Jean Rommelaere, Celina Cziepluch
The human small glutamine-rich TPR-containing protein (hSGT) is essential for cell division since RNA-interference-mediated strong reduction of hSGT protein levels causes mitotic arrest (M. Winnefeld, J. Rommelaere, and C. Cziepluch, The human small glutamine-rich TPR-containing protein is required for progress through cell division, Exp. Cell Res. 293 (2004), 43-57). Analysis of HeLa cells expressing a histone 2A-YFP fusion protein revealed the continuous presence of few mislocalized chromosomes close to the spindle poles as possible cause for hSGT depletion-dependent prometaphase arrest...
August 1, 2006: Experimental Cell Research
https://www.readbyqxmd.com/read/12720548/p300-cbp-associated-factor-p-caf-interacts-with-nuclear-respiratory-factor-1-to-regulate-the-udp-n-acetyl-alpha-d-galactosamine-polypeptide-n-acetylgalactosaminyltransferase-3-gene
#4
Hiroto Izumi, Ryo Ohta, Gunji Nagatani, Tomoko Ise, Yoshifumi Nakayama, Minoru Nomoto, Kimitoshi Kohno
We demonstrated recently that expression of the UDP- N -acetyl-alpha-D-galactosamine: polypeptide N -acetylgalactosaminyltrans-ferase-3 (GalNAc-T3) gene is restricted to epithelial glands [Nomoto, Izumi, Ise, Kato, Takano, Nagatani, Shibao, Ohta, Imamura, Kuwano, Matsuo, Yamada, Itoh and Kohno (1999) Cancer Res. 59, 6214-6222]. In the present study, we show that sodium butyrate treatment of human breast cancer MCF-7 cells transcriptionally activates the GalNAc-T3 gene. Transient transfection of plasmids containing a reporter gene under the control of GalNAc-T3 indicated that several transcriptional elements are involved in response to sodium butyrate, with the nuclear respiratory factor-1 (NRF-1)-binding motif located between -88 and -77nt being the most important...
August 1, 2003: Biochemical Journal
https://www.readbyqxmd.com/read/11074125/viral-genome-organizer-a-system-for-analyzing-complete-viral-genomes
#5
C Upton, D Hogg, D Perrin, M Boone, N L Harris
The viral genome organizer (VGO) is designed to simplify the characterization and annotation of complete viral genomes (particularly those of large poxviruses) and to help researchers discover new genes and detect gene fragmentation. VGO is based on Genotator [Harris, N.L., 1997. Genome Res. 7, 754-762], an annotation workbench designed for the analysis of eukaryotic genomic sequences. VGO automates a number of database search routines (FASTA, BLASTP, PSI-BLAST and TBLASTN), processes the results through a multiple-alignment viewer (MView; [Brown, N...
September 2000: Virus Research
https://www.readbyqxmd.com/read/10716733/oligomerization-of-serotonin-transporter-and-its-functional-consequences
#6
F Kilic, G Rudnick
Two forms of serotonin transporter (SERT) were prepared with different epitope tags. When co-expressed in HeLa cells, the form containing a FLAG tag (Res-FLAG) was associated with the form containing a c-myc tag (Sens-myc). Antibody against c-myc precipitated Res-FLAG from detergent extracts of cells expressing both forms, but not when Res-FLAG was expressed alone. The specificity of the interaction was demonstrated by the observation that anti-myc antibodies did not precipitate the unrelated vesicular stomatitis virus coat glycoprotein when it was co-expressed with Sens-myc...
March 28, 2000: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/8132587/proteolytic-cleavage-of-vaccinia-virus-virion-proteins-mutational-analysis-of-the-specificity-determinants
#7
P Lee, D E Hruby
Previous studies have suggested that cleavage of vaccinia virus core protein precursors occurs within the consensus tripeptide motif -A-G decreases X-. As an approach to delineate the sequence and structural features of the precursor polypeptides that are responsible for directing site-specific scission within this element, site-directed mutagenesis procedures were employed in concert with an in vivo trans-processing assay of the P25K: FLAG reporter plasmid. The results obtained suggest that residue occupancy at the P1' site (following the nomenclature of Schechter and Berger (Schechter, I...
March 18, 1994: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/7848273/heterologous-expression-in-escherichia-coli-of-native-and-mutant-forms-of-the-major-intrinsic-protein-of-rat-eye-lens-mip26
#8
N Dilsiz, M J Crabbe
The complete cDNA of rat eye lens major intrinsic protein (MIP26) was sequenced using the dideoxy chain termination method. The sequence displayed 89% nucleotide identity and 95% identity at the amino acid level with bovine MIP26 [Gorin, Yancey, Cline, Revel and Horwitz (1984) Cell, 39, 49-54]. Both native and mutant cDNAs coding for rat MIP26 were amplified by PCR and subcloned into the pPOW expression vector for expression of Escherichia coli. A membrane signal peptide (PelB) was used for secretion of MIP26 into the cytoplasmic membrane...
February 1, 1995: Biochemical Journal
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