Josiane Lilian Dos Santos Schiavinato, Lucila Habib Bourguignon Oliveira, Amélia Goes Araujo, Maristela Delgado Orellana, Patrícia Viana Bonini de Palma, Dimas Tadeu Covas, Marco Antonio Zago, Rodrigo Alexandre Panepucci
During the early thymus colonization, Notch signaling activation on hematopoietic progenitor cells (HPCs) drives proliferation and T cell commitment. Although these processes are driven by transcription factors such as HOXB4 and GATA3, there is no evidence that Notch directly regulates their transcription. To evaluate the role of NOTCH and TNF signaling in this process, human CD34+ HPCs were cocultured with OP9-DL1 cells, in the presence or absence of TNF. The use of a Notch signaling inhibitor and a protein synthesis inhibitor allowed us to distinguish primary effects, mediated by direct signaling downstream Notch and TNF, from secondary effects, mediated by de novo synthesized proteins...
October 2016: In Vitro Cellular & Developmental Biology. Animal