keyword
MENU ▼
Read by QxMD icon Read
search

zuniga-pflucker

keyword
https://www.readbyqxmd.com/read/27789110/t-cell-genesis-in-vitro-veritas-est
#1
REVIEW
Patrick M Brauer, Jastaranpreet Singh, Sintia Xhiku, Juan Carlos Zúñiga-Pflücker
T cells, as orchestrators of the adaptive immune response, serve important physiological and potentially therapeutic roles, for example in cancer immunotherapy. T cells are readily isolated from patients; however, the yield of antigen-specific T cells is limited, thus making their clinical use challenging. Therefore, the generation of T lymphocytes from hematopoietic stem/progenitor cells (HSPCs) and human pluripotent stem cells (PSCs) in vitro provides an attractive method for the large-scale production and genetic manipulation of T cells...
December 2016: Trends in Immunology
https://www.readbyqxmd.com/read/27301863/modeling-altered-t-cell-development-with-induced-pluripotent-stem-cells-from-patients-with-rag1-dependent-immune-deficiencies
#2
Patrick M Brauer, Itai M Pessach, Erik Clarke, Jared H Rowe, Lisa Ott de Bruin, Yu Nee Lee, Carmen Dominguez-Brauer, Anne M Comeau, Geneve Awong, Kerstin Felgentreff, Yuhang H Zhang, Andrea Bredemeyer, Waleed Al-Herz, Likun Du, Francesca Ververs, Marion Kennedy, Silvia Giliani, Gordon Keller, Barry P Sleckman, David G Schatz, Frederic D Bushman, Luigi D Notarangelo, Juan Carlos Zúñiga-Pflücker
Primary immunodeficiency diseases comprise a group of heterogeneous genetic defects that affect immune system development and/or function. Here we use in vitro differentiation of human induced pluripotent stem cells (iPSCs) generated from patients with different recombination-activating gene 1 (RAG1) mutations to assess T-cell development and T-cell receptor (TCR) V(D)J recombination. RAG1-mutants from severe combined immunodeficient (SCID) patient cells showed a failure to sustain progression beyond the CD3(--)CD4(-)CD8(-)CD7(+)CD5(+)CD38(-)CD31(-/lo)CD45RA(+) stage of T-cell development to reach the CD3(-/+)CD4(+)CD8(+)CD7(+)CD5(+)CD38(+)CD31(+)CD45RA(-) stage...
August 11, 2016: Blood
https://www.readbyqxmd.com/read/27297795/high-content-screening-identifies-kinase-inhibitors-that-overcome-venetoclax-resistance-in-activated-cll-cells
#3
Sina Oppermann, Jarkko Ylanko, Yonghong Shi, Santosh Hariharan, Christopher C Oakes, Patrick M Brauer, Juan C Zúñiga-Pflücker, Brian Leber, David E Spaner, David W Andrews
Novel agents such as the Bcl-2 inhibitor venetoclax (ABT-199) are changing treatment paradigms for chronic lymphocytic leukemia (CLL) but important problems remain. Although some patients exhibit deep and durable responses to venetoclax as a single agent, other patients harbor subpopulations of resistant leukemia cells that mediate disease recurrence. One hypothesis for the origin of resistance to venetoclax is by kinase-mediated survival signals encountered in proliferation centers that may be unique for individual patients...
August 18, 2016: Blood
https://www.readbyqxmd.com/read/26294407/induction-of-t-cell-development-in-vitro-by-delta-like-dll-expressing-stromal-cells
#4
Mahmood Mohtashami, Payam Zarin, Juan Carlos Zúñiga-Pflücker
Recreating the thymic microenvironment in vitro poses a great challenge to immunologists. Until recently, the only approach was to utilize the thymic tissue in its three-dimensional form and to transfer the hematopoietic progenitors into this tissue to generate de novo T cells. With the advent of OP9-DL cells (bone marrow-derived cells that are transduced to express Notch ligand, Delta-like), hematopoietic stem cells (HSC) could be induced to differentiate into T cells in culture for the first time outside of the thymic tissue on a monolayer...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/26227158/in-vitro-t-cell-generation-from-adult-embryonic-and-induced-pluripotent-stem-cells-many-roads-to-one-destination
#5
REVIEW
Michelle J Smith, Beau R Webber, Mahmood Mohtashami, Heather E Stefanski, Juan Carlos Zúñiga-Pflücker, Bruce R Blazar
T lymphocytes are critical mediators of the adaptive immune system and have the capacity to serve as therapeutic agents in the areas of transplant and cancer immunotherapy. While T cells can be isolated and expanded from patients, T cells derived in vitro from both hematopoietic stem/progenitor cells (HSPCs) and human pluripotent stem cells (hPSCs) offer great potential advantages in generating a self-renewing source of T cells that can be readily genetically modified. T-cell differentiation in vivo is a complex process requiring tightly regulated signals; providing the correct signals in vitro to induce T-cell lineage commitment followed by their development into mature, functional, single positive T cells, is similarly complex...
November 2015: Stem Cells
https://www.readbyqxmd.com/read/25941319/t-cell-development-runs-marrow-deep
#6
COMMENT
Juan Carlos Zúñiga-Pflücker
No abstract text is available yet for this article.
May 4, 2015: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/25889009/hematopoiesis-from-start-to-immune-reconstitution-potential
#7
REVIEW
Haydn C-Y Liang, Juan Carlos Zúñiga-Pflücker
The study of hematopoiesis has been a focus for developmental biologists for over 100 years. What started as a series of microscopic observations in different animal model systems has since evolved into studies of gene expression and regulation, and subsequent protein-protein interactions, cell surface protein expression profiling, and functional mapping of cell fates. In this review, we will discuss the milestone discoveries that have been achieved in the field of hematopoietic development, as well as the techniques that have been employed...
April 11, 2015: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/25866401/gamma-delta-t-cell-differentiation-and-effector-function-programming-tcr-signal-strength-when-and-how-much
#8
REVIEW
Payam Zarin, Edward L Y Chen, Tracy S H In, Michele K Anderson, Juan Carlos Zúñiga-Pflücker
γδ T-cells boast an impressive functional repertoire that can paint them as either champions or villains depending on the environmental and immunological cues. Understanding the function of the various effector γδ subsets necessitates tracing the developmental program of these subsets, including the point of lineage bifurcation from αβ T-cells. Here, we review the importance of signals from the T-cell receptor (TCR) in determining αβ versus γδ lineage fate, and further discuss how the molecular components of this pathway may influence the developmental programming of γδ T-cells functional subsets...
July 2015: Cellular Immunology
https://www.readbyqxmd.com/read/25526308/noncanonical-mode-of-erk-action-controls-alternative-%C3%AE-%C3%AE-and-%C3%AE-%C3%AE-t-cell-lineage-fates
#9
Sang-Yun Lee, Francis Coffey, Shawn P Fahl, Suraj Peri, Michele Rhodes, Kathy Q Cai, Michael Carleton, Stephen M Hedrick, Hans Joerg Fehling, Juan Carlos Zúñiga-Pflücker, Dietmar J Kappes, David L Wiest
Gradations in extracellular regulated kinase (ERK) signaling have been implicated in essentially every developmental checkpoint or differentiation process encountered by lymphocytes. Yet, despite intensive effort, the molecular basis by which differences in ERK activation specify alternative cell fates remains poorly understood. We report here that differential ERK signaling controls lymphoid-fate specification through an alternative mode of action. While ERK phosphorylates most substrates, such as RSK, by targeting them through its D-domain, this well-studied mode of ERK action was dispensable for development of γδ T cells...
December 18, 2014: Immunity
https://www.readbyqxmd.com/read/25517607/dedicated-mtec-progenitors-stay-true-even-into-adulthood
#10
COMMENT
Korosh Kianizad, Juan Carlos Zúñiga-Pflücker
Knowledge about the cells giving rise to and maintaining the thymic structure remains limited. In this issue of Immunity, Sekai et al. (2014) identify a postnatal self-renewing unipotential progenitor population capable of generating thymic medullary cells and lay the foundation for research into thymic regeneration.
November 20, 2014: Immunity
https://www.readbyqxmd.com/read/25357997/a-monoclonal-antibody-against-the-extracellular-domain-of-mouse-and-human-epithelial-v-like-antigen-1-reveals-a-restricted-expression-pattern-among-cd4-cd8-thymocytes
#11
Nahir Garabatos, Jesus Blanco, Cesar Fandos, Elena Lopez, Pere Santamaria, Andrea Ruiz, Maria Laura Perez-Vidakovics, Patricia Benveniste, Oleksandr Galkin, Juan Carlos Zuñiga-Pflucker, Pau Serra
Expression of transcripts for the homotypic adhesion protein epithelial V-like antigen 1 (EVA1), also known as myelin protein zero like-2 (Mpzl2), is known to be present in thymic stromal cells. However, protein expression within different thymic subsets, stromal and/or lymphoid, has not been characterized due a lack of specific reagents. To address this, we generated a hybridoma (G9P3-1) secreting a monoclonal antibody (G9P3-1Mab), reactive against both human and mouse EVA1. The G9P3-1Mab was generated by immunizing Mpzl2-deficient gene-targeted mice with the extracellular domain of EVA1, followed by a conventional hybridoma fusion protocol, illustrating the feasibility of using gene-targeted mice to generate monoclonal antibodies with multiple species cross-reactivity...
October 2014: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/25349888/derivation-of-t-cells-in-vitro-from-mouse-embryonic-stem-cells
#12
Martina Kučerová-Levisohn, Jordana Lovett, Armin Lahiji, Roxanne Holmes, Juan Carlos Zúñiga-Pflücker, Benjamin D Ortiz
The OP9/OP9-DL1 co-culture system has become a well-established method for deriving differentiated blood cell types from embryonic and hematopoietic progenitors of both mouse and human origin. It is now used to address a growing variety of complex genetic, cellular and molecular questions related to hematopoiesis, and is at the cutting edge of efforts to translate these basic findings to therapeutic applications. The procedures are straightforward and routinely yield robust results. However, achieving successful hematopoietic differentiation in vitro requires special attention to the details of reagent and cell culture maintenance...
2014: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/25138665/an-in-vitro-model-of-innate-lymphoid-cell-function-and-differentiation
#13
D S J Allan, C L Kirkham, O A Aguilar, L C Qu, P Chen, J H Fine, P Serra, G Awong, J L Gommerman, J C Zúñiga-Pflücker, J R Carlyle
Innate lymphoid cells (ILC) are RAG-independent lymphocytes with important roles in innate immunity, and include group-1 (natural killer (NK) cell, ILC1), group-2 (ILC2), and group-3 (lymphoid tissue inducer (LTi), NCR(+) ILC3) subsets. Group-3 ILC express Rorγt, produce interleukin (IL)-22, and are critically important in the normal function of mucosal tissues. Here, we describe a novel model cell line for the study of ILC function and differentiation. The parental MNK cell line, derived from NKR-P1B(+) fetal thymocytes, shows a capacity to differentiate in γc cytokines...
March 2015: Mucosal Immunology
https://www.readbyqxmd.com/read/24936476/foxn1-gfp-w-reporter-hescs-enable-identification-of-integrin-%C3%AE-4-hla-dr-and-epcam-as-markers-of-human-psc-derived-foxn1-thymic-epithelial-progenitors
#14
Chew-Li Soh, Antonietta Giudice, Robert A Jenny, David A Elliott, Tanya Hatzistavrou, Suzanne J Micallef, Korosh Kianizad, Natalie Seach, Juan Carlos Zúñiga-Pflücker, Ann P Chidgey, Alan Trounson, Susan K Nilsson, David N Haylock, Richard L Boyd, Andrew G Elefanty, Edouard G Stanley
Thymic epithelial cells (TECs) play a critical role in T cell maturation and tolerance induction. The generation of TECs from in vitro differentiation of human pluripotent stem cells (PSCs) provides a platform on which to study the mechanisms of this interaction and has implications for immune reconstitution. To facilitate analysis of PSC-derived TECs, we generated hESC reporter lines in which sequences encoding GFP were targeted to FOXN1, a gene required for TEC development. Using this FOXN1 (GFP/w) line as a readout, we developed a reproducible protocol for generating FOXN1-GFP(+) thymic endoderm cells...
June 3, 2014: Stem Cell Reports
https://www.readbyqxmd.com/read/24799532/new-role-identified-for-lrr-containing-proteins-in-t-cell-development
#15
COMMENT
Juan-Carlos Zúñiga-Pflücker
No abstract text is available yet for this article.
May 5, 2014: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/24748636/an-overview-of-the-intrathymic-intricacies-of-t-cell-development
#16
REVIEW
Divya K Shah, Juan Carlos Zúñiga-Pflücker
The generation of a functional and diverse repertoire of T cells occurs in the thymus from precursors arriving from the bone marrow. In this article, we introduce the various stages of mouse thymocyte development and highlight recent work using various in vivo, and, where appropriate, in vitro models of T cell development that led to discoveries in the regulation afforded by transcription factors and receptor-ligand signaling pathways in specifying, maintaining, and promoting the T cell lineage and the production of T cells...
May 1, 2014: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/24706811/enforcement-of-%C3%AE-%C3%AE-lineage-commitment-by-the-pre-t-cell-receptor-in-precursors-with-weak-%C3%AE-%C3%AE-tcr-signals
#17
Payam Zarin, Gladys W Wong, Mahmood Mohtashami, David L Wiest, Juan Carlos Zúñiga-Pflücker
Developing thymocytes bifurcate from a bipotent precursor into αβ- or γδ-lineage T cells. Considering this common origin and the fact that the T-cell receptor (TCR) β-, γ-, and δ-chains simultaneously rearrange at the double negative (DN) stage of development, the possibility exists that a given DN cell can express and transmit signals through both the pre-TCR and γδ-TCR. Here, we tested this scenario by defining the differentiation outcomes and criteria for lineage choice when both TCR-β and γδ-TCR are simultaneously expressed in Rag2(-/-) DN cells via retroviral transduction...
April 15, 2014: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/24681242/adapting-in-vitro-embryonic-stem-cell-differentiation-to-the-study-of-locus-control-regions
#18
Armin Lahiji, Martina Kučerová-Levisohn, Roxanne Holmes, Juan Carlos Zúñiga-Pflücker, Benjamin D Ortiz
Numerous locus control region (LCR) activities have been discovered in gene loci important to immune cell development and function. LCRs are a distinct class of cis-acting gene regulatory elements that appear to contain all the DNA sequence information required to establish an independently and predictably regulated gene expression program at any genomic site in native chromatin of a whole animal. As such, LCR-regulated transgenic reporter systems provide invaluable opportunities to investigate the mechanisms of gene regulatory DNA action during development...
May 2014: Journal of Immunological Methods
https://www.readbyqxmd.com/read/24584061/leukocyte-infiltration-and-activation-of-the-nlrp3-inflammasome-in-white-adipose-tissue-following-thermal-injury
#19
Mile Stanojcic, Peter Chen, Rachael A Harrison, Vivian Wang, Jeremy Antonyshyn, Juan Carlos Zúñiga-Pflücker, Marc G Jeschke
OBJECTIVES: Severe thermal injury is associated with extreme and prolonged inflammatory and hypermetabolic responses, resulting in significant catabolism that delays recovery or even leads to multiple organ failure and death. Burned patients exhibit many symptoms of stress-induced diabetes, including hyperglycemia, hyperinsulinemia, and hyperlipidemia. Recently, the nucleotide-binding domain, leucine-rich family (NLR), pyrin-containing 3 (NLRP3) inflammasome has received much attention as the sensor of endogenous "danger signals" and mediator of "sterile inflammation" in type II diabetes...
June 2014: Critical Care Medicine
https://www.readbyqxmd.com/read/24493796/the-tcr-ligand-inducible-expression-of-cd73-marks-%C3%AE-%C3%AE-lineage-commitment-and-a-metastable-intermediate-in-effector-specification
#20
Francis Coffey, Sang-Yun Lee, Terkild B Buus, Jens-Peter Holst Lauritsen, Gladys W Wong, Michelle L Joachims, Linda F Thompson, Juan Carlos Zúñiga-Pflücker, Dietmar J Kappes, David L Wiest
Numerous studies indicate that γδ T cell receptor (γδTCR) expression alone does not reliably mark commitment of early thymic progenitors to the γδ fate. This raises the possibility that the γδTCR is unable to intrinsically specify fate and instead requires additional environmental factors, including TCR-ligand engagement. We use single cell progenitor assays to reveal that ligand acts instructionally to direct adoption of the γδ fate. Moreover, we identify CD73 as a TCR ligand-induced cell surface protein that distinguishes γδTCR-expressing CD4(-)CD8(-) progenitors that have committed to the γδ fate from those that have not yet done so...
February 10, 2014: Journal of Experimental Medicine
keyword
keyword
62208
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"