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https://www.readbyqxmd.com/read/27936054/high-prevalence-of-faecal-carriage-of-esbl-producing-enterobacteriaceae-among-children-in-dar-es-salaam-tanzania
#1
Marit G Tellevik, Bjørn Blomberg, Øyvind Kommedal, Samuel Y Maselle, Nina Langeland, Sabrina J Moyo
BACKGROUND: Faecal carriage of ESBL-producing bacteria is a potential risk for transmission and infection. Little is known about faecal carriage of antibiotic resistance in Tanzania. This study aimed to investigate the prevalence of faecal carriage of ESBL-producing Enterobacteriaceae and to identify risk factors for carriage among young children in Tanzania. METHODOLOGY/PRINCIPAL FINDINGS: From August 2010 to July 2011, children below 2 years of age were recruited in Dar es Salaam, including healthy community children (n = 250) and children hospitalized due to diarrhoea (n = 250) or other diseases (n = 103)...
2016: PloS One
https://www.readbyqxmd.com/read/27872719/phylogenetic-typing-and-detection-of-extended-spectrum-%C3%AE-lactamases-in-escherichia-coli-isolates-from-broiler-chickens-in-ahvaz-iran
#2
Ramezan Ali Jafari, Hossein Motamedi, Elham Maleki, Reza Ghanbarpour, Mansoor Mayahi
This study was conducted to reveal the phylogenetic background, to detect the genes encoding TEM, SHV and CTX-M-15 extended-spectrum β-lactamases (ESBL), and to analyze their distribution in phylo-groups of 150 Escherichia coli isolates from broiler chickens in Ahvaz (Southwest of Iran). Seventy- five cloacal swabs from healthy birds (fecal isolates), and 75 heart blood samples from birds with colibacillosis (septicemic isolates) were obtained. All isolates were phylotyped and screened for ESBL genes by polymerase chain reaction (PCR)...
2016: Veterinary Research Forum
https://www.readbyqxmd.com/read/27856044/community-fecal-carriage-of-broad-spectrum-cephalosporin-resistant-escherichia-coli-in-tunisian-children
#3
Sana Ferjani, Mabrouka Saidani, Zeineb Hamzaoui, Carla Andrea Alonso, Carmen Torres, Elaa Maamar, Amine Faouzi Slim, Ben Boubaker Ilhem Boutiba
The spread of extended spectrum β-lactamases (ESBL) and plasmid mediated AmpC β-lactamases (pAmpC) was evaluated in Escherichia coli strains collected from the intestinal microbiota of healthy children in Tunisia. The carriage rate of CTX(R)E. coli was 6.6% (7 of 105 samples) and one strain/sample was further characterized (7 isolates). These isolates harbored blaCTX-M-1 (n = 4), blaCTX-M-15 (n = 2), and blaCMY-2 gene (n = 1), which were usually located on FIB replicon type and carried class 1 integrons. The acc(6')-Ib-cr variant was identified in one isolate that harbored blaCTX-M-15...
March 8, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27798205/community-carriage-of-esbl-producing-escherichia-coli-is-associated-with-strains-of-low-pathogenicity-a-swedish-nationwide-study
#4
Sofia Ny, Sonja Löfmark, Stefan Börjesson, Stina Englund, Maj Ringman, Jakob Bergström, Pontus Nauclér, Christian G Giske, Sara Byfors
OBJECTIVES: Community carriage of ESBL-producing Escherichia coli (EPE) is common worldwide and there is a need to understand the connection between carriage and infection. We compared the molecular characteristics of EPE among Swedish community carriers with those of EPE causing invasive infections. METHODS: We collected 2134 faecal samples from randomly selected Swedish inhabitants and examined them for the presence of EPE. All participating volunteers answered a questionnaire about putative risk factors for EPE carriage...
October 24, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27712137/an-update-on-the-management-of-urinary-tract-infections-in-the-era-of-antimicrobial-resistance
#5
Mazen S Bader, Mark Loeb, Annie A Brooks
Urinary tract infections (UTIs) caused by antibiotic-resistant Gram-negative bacteria are a growing concern due to limited therapeutic options. Gram-negative bacteria, specifically Enterobacteriaceae, are common causes of both community-acquired and hospital acquired UTIs. These organisms can acquire genes that encode for multiple antibiotic resistance mechanisms, including extended-spectrum-lactamases (ESBLs), AmpC- β -lactamase, and carbapenemases. The assessment of suspected UTI includes identification of characteristic symptoms or signs, urinalysis, dipstick or microscopic tests, and urine culture if indicated...
October 7, 2016: Postgraduate Medicine
https://www.readbyqxmd.com/read/27694727/multidrug-resistant-esbl-producing-enterobacteriaceae-and-associated-risk-factors-in-community-infants-in-lebanon
#6
Soumaya Moustafa Hijazi, Mohamad Anwar Fawzi, Faten Moustafa Ali, Khaled Hussein Abd El Galil
INTRODUCTION: Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) infections are a growing threat to children, and the treatment of these infections becomes more and more challenging. A huge reservoir for ESBLs in the community is the fecal flora of children. This study investigates the rectal colonization, associated risk factors, antimicrobial susceptibility, and molecular characterization of ESBL-PE in Lebanese community infants. METHODOLOGY: A total of 117 rectal swabs were taken from healthy infants between 1 and 12 months of age...
September 30, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/27681363/prevalence-and-quinolone-resistance-of-fecal-carriage-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-in-6-communities-and-2-physical-examination-center-populations-in-shanghai-china
#7
Qi Ni, Yuan Tian, Lihua Zhang, Cen Jiang, Danfeng Dong, Zhen Li, Enqiang Mao, Yibing Peng
OBJECTIVES: To characterize extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates from the community, determine their antibiotic sensitivity profiles and quinolone resistance mechanisms, and identify any horizontal transfer of ESBL genes. METHODS: One thousand seven hundred thirty-two stool samples were collected from healthy individuals in 6 communities and 2 physical examination centers in Shanghai, China. ESBL-producing E. coli was screened and confirmed by confirmatory test and E...
July 12, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27530858/faecal-carriage-rate-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-in-hospitalised-patients-and-healthy-asymptomatic-individuals-coming-for-health-check-up
#8
Rachana Babu, Anil Kumar, Shamsul Karim, Sruthi Warrier, Suresh G Nair, Sanjeev K Singh, Raja Biswas
The prevalence of extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) in hospitalised and community patients is of significant public health concern. The aim of this study was to estimate the faecal carriage rate of ESBL-PE in hospitalised patients and healthy asymptomatic individuals coming for health check-up. Non-repetitive, consecutive stool samples from 480 adults (260 healthy individuals and 220 hospitalised patients) aged ≥18 years from November 2011 to July 2013 were screened using MacConkey agar supplemented with ceftazidime...
September 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27530851/molecular-characterisation-of-extended-spectrum-%C3%AE-lactamase-and-plasmid-ampc-producing-escherichia-coli-strains-isolated-from-broilers-in-b%C3%A3-ja%C3%A3-a-algeria
#9
Mohamed Belmahdi, Sofiane Bakour, Charbel Al Bayssari, Abdelaziz Touati, Jean-Marc Rolain
This study aimed to characterise the molecular support of antibiotic resistance in expanded-spectrum cephalosporin (ESC)-resistant Escherichia coli isolates recovered from healthy broilers in Béjaïa, northeast Algeria. A total of 61 intestinal swabs from slaughtered broilers from four regions in Béjaïa locality, Algeria, were collected between February and April 2014, from which 20 ESC-resistant E. coli strains were isolated. Escherichia coli isolates were identified by classical biochemical and MALDI-TOF methods...
September 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27473782/molecular-signature-of-extended-spectrum-%C3%AE-lactamase-producing-klebsiella-pneumoniae-isolated-from-bovine-milk-in-eastern-and-north-eastern-india
#10
S Koovapra, S Bandyopadhyay, G Das, D Bhattacharyya, J Banerjee, A Mahanti, I Samanta, P K Nanda, A Kumar, R Mukherjee, U Dimri, R K Singh
The present study reports on 23 extended spectrum β-lactamase producing Klebsiella pneumoniae (KP), isolated from milk samples (n=340) of healthy cows (n=129) and cows with subclinical (n=159) and clinical (n=52) mastitis, from three different states of India viz. West Bengal, Jharkhand and Mizoram. Seven of them were AmpC type β-lactamase producers, as well. The ESBL producing KP were significantly (P=0.006, χ2=10.04, df=2) and more frequently detected in milk samples of mastitic cows than healthy ones...
October 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27462305/polyclonal-intestinal-colonization-with-extended-spectrum-cephalosporin-resistant-enterobacteriaceae-upon-traveling-to-india
#11
João Pires, Esther Kuenzli, Sara Kasraian, Regula Tinguely, Hansjakob Furrer, Markus Hilty, Christoph Hatz, Andrea Endimiani
We aimed to assess the intestinal colonization dynamics by multiple extended-spectrum cephalosporin-resistant Enterobacteriaceae (ESC-R-Ent) clones in Swiss travelers to India, a country with high prevalence of these multidrug-resistant pathogens. Fifteen healthy volunteers (HVs) colonized with ESC-R-Ent after traveling to India who provided stools before, after, and at 3- and 6-month follow-up are presented in this study. Stools were enriched in a LB broth containing 3 mg/L cefuroxime and plated in standard selective media (BLSE, ChromID ESBL, Supercarba) to detect carbapenem- and/or ESC-R-Ent...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27458435/surveillance-of-extended-spectrum-beta-lactamase-producing-escherichia-coli-in-dairy-cattle-farms-in-the-nile-delta-egypt
#12
Sascha D Braun, Marwa F E Ahmed, Hosny El-Adawy, Helmut Hotzel, Ines Engelmann, Daniel Weiß, Stefan Monecke, Ralf Ehricht
INTRODUCTION: Industrial livestock farming is a possible source of multi-resistant Gram-negative bacteria, including producers of extended spectrum beta-lactamases (ESBLs) conferring resistance to 3rd generation cephalosporins. Limited information is currently available on the situation of ESBL producers in livestock farming outside of Western Europe. A surveillance study was conducted from January to May in 2014 in four dairy cattle farms in different areas of the Nile delta, Egypt. MATERIALS AND METHODS: In total, 266 samples were collected from 4 dairy farms including rectal swabs from clinically healthy cattle (n = 210), and environmental samples from the stalls (n = 56)...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27404368/high-rate-of-faecal-carriage-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-in-healthy-children-in-bangui-central-african-republic
#13
A Farra, T Frank, L Tondeur, P Bata, J C Gody, M Onambele, C Rafaï, M Vray, S Breurec
The aim of this study was to estimate the prevalence of extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) in faeces of healthy children aged 0-59 months in Bangui (Central African Republic). Stool samples of 134 children, recruited for a matched case-control study, were cultured on a commercial ESBL-selective chromogenic medium (CHROMagar ESBL, France). The phenotypic resistance patterns of isolated strains were investigated, as well as the genetic basis for antibiotic resistance. The factors associated with increased risk for ESBL-E carriage were also studied...
July 9, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27357036/prevalence-and-antibiotic-resistance-profiles-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolated-from-healthy-broilers-in-shandong-province-china
#14
Song Li, Miaomiao Zhao, Junhe Liu, Yufa Zhou, Zengmin Miao
Food-producing animals carrying extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) have posed a potential threat to human and animal health. However, information regarding ESBL-EC in the intensive broiler breeding areas of Shandong Province, People's Republic of China, is very limited. The goal of our study was to investigate the prevalence and drug resistance characteristics of ESBL-EC in healthy broilers from Shandong Province. A total of 142 ESBL-EC isolates were collected from four prefectures in Shandong Province from October 2014 to February 2015...
July 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/27330043/evidence-of-household-transfer-of-esbl-pampc-producing-enterobacteriaceae-between-humans-and-dogs-a-pilot-study
#15
Oskar Ljungquist, Ditte Ljungquist, Mattias Myrenås, Cecilia Rydén, Maria Finn, Björn Bengtsson
BACKGROUND: Extended-spectrum cephalosporin-resistant Enterobacteriaceae (ESCRE) are an increasing healthcare problem in both human and veterinary medicine. The spread of ESCRE is complex with multiple reservoirs and different transmission routes. The aim of this study was to investigate if ESCRE carriage in dogs is more prevalent in households with a known human carrier, compared to households where humans are known to be negative for ESCRE. Identical ESCRE strains in humans and dogs of the same household would suggest a possible spread between humans and dogs...
2016: Infection Ecology & Epidemiology
https://www.readbyqxmd.com/read/27305591/high-prevalence-of-non-st131-ctx-m-15-producing-escherichia-coli-in-healthy-cattle-in-lebanon
#16
Mohamad Diab, Monzer Hamze, Jean-Yves Madec, Marisa Haenni
Extended-Spectrum Beta-Lactamases (ESBL)-producing Escherichia coli have disseminated in both humans and animals worldwide. However, the ESBL epidemiology in these two reservoirs differs markedly, with CTX-M-15 frequently found in humans and CTX-M-1 preferentially found in animals. Our goal was to estimate the prevalence of fecal carriage of ESBL producers in cattle from 31 farms in Lebanon and to characterize the responsible enzymes. This prevalence was high (26/31, 84% of ESBL-positive farms), with a majority of isolates producing CTX-M-15 (27/40, 67...
June 15, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27220012/high-prevalence-of-extended-spectrum-and-plasmidic-ampc-beta-lactamase-producing-escherichia-coli-from-poultry-in-tunisia
#17
Elaa Maamar, Samia Hammami, Carla Andrea Alonso, Nouha Dakhli, Mohamed Salah Abbassi, Sana Ferjani, Zaineb Hamzaoui, Mabrouka Saidani, Carmen Torres, Ilhem Boutiba-Ben Boubaker
This study was conducted to detect extended spectrum beta-lactamases (ESBLs) and plasmidic AmpC beta-lactamase (pAmpC-BL)-producing Escherichia coli isolates in industrial poultry samples were collected from healthy chickens of the three farms. Samples were inoculated onto desoxycholate-lactose-agar plates supplemented with cefotaxime (2mg/L). E. coli was identified by biochemical and molecular methods and antibiotic susceptibility testing by the disk diffusion method. Genes encoding ESBLs and pAmpC-BL were detected by PCR and sequencing...
August 16, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27208763/the-continuing-plague-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-infections
#18
REVIEW
Amos Adler, David E Katz, Dror Marchaim
Antimicrobial resistance is a common iatrogenic complication of modern life and medical care. One of the most demonstrative examples is the exponential increase in the incidence of extended-spectrum β-lactamases (ESBLs) production among Enterobacteriaceae, which is the most common human pathogens outside of the hospital settings. Infections resulting from ESBL-producing bacteria are associated with devastating outcomes, now affecting even previously healthy individuals. This development poses an enormous burden and threat to public health...
June 2016: Infectious Disease Clinics of North America
https://www.readbyqxmd.com/read/27180312/beta-lactamases-in-enterobacteriaceae-infections-in-children
#19
Christopher Alan Moxon, Stéphane Paulus
Multi-drug resistance in Gram negative bacteria, particularly in Enterobacteriaceae, is a major clinical and public health challenge. The main mechanism of resistance in Enterobacteriaceae is linked to the production of beta-lactamase hydrolysing enzymes such as extended spectrum beta-lactamases (ESBL), AmpC beta-lactamases and carbapenemases (Carbapenemase Producing Enterobacteriaceae (CPE)). ESBL and CPE resistance genes are located on plasmids, which can be transmitted between Enterobacteriaceae, facilitating their spread in hospitals and communities...
July 5, 2016: Journal of Infection
https://www.readbyqxmd.com/read/27143671/fecal-colonization-with-extended-spectrum-beta-lactamase-producing-enterobacteriaceae-and-risk-factors-among-healthy-individuals-a-systematic-review-and-metaanalysis
#20
Styliani Karanika, Theodoros Karantanos, Marios Arvanitis, Christos Grigoras, Eleftherios Mylonakis
BACKGROUND: Gut colonization is a risk factor for infections with extended-spectrum beta-lactamase (ESBL)-producing organisms. We aimed to determine the ESBL class A reservoir among healthy individuals. METHODS: We searched PubMed and EMBASE (through 10 July 2015) looking for studies that contained data for fecal colonization with ESBL class A bacteria among healthy individuals for each World Health Organization-defined region. Distribution of isolates among cefotaximase (CTX-M), sulfhydryl variable, and temoneira enzymes and data on previous antibiotic use, international travel, previous hospitalization, and animal contacts were extracted...
August 1, 2016: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
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