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https://www.readbyqxmd.com/read/29644511/virulence-associated-genes-and-antibiotic-susceptibility-among-vaginal-and-rectal-escherichia-coli-isolates-from-healthy-pregnant-women-in-poland
#1
Agnieszka Kaczmarek, Krzysztof Skowron, Anna Budzyńska, Eugenia Gospodarek-Komkowska
Vaginal and/or rectal Escherichia coli colonization of pregnant women is sometimes associated with neonatal infections. Despite the relevance of these strains, they have been rarely described before. Thus, the aim of this study was to compare vaginal (VEC) and rectal E. coli (REC) isolates in respect of antimicrobial susceptibility and the frequency of virulence-associated genes (VAGs). The antimicrobial susceptibility of 50 VEC and 50 REC isolates was performed by using the disc diffusion method, and VAGs were detected by PCR...
April 12, 2018: Folia Microbiologica
https://www.readbyqxmd.com/read/29575785/extended-spectrum-beta-lactamase-producing-escherichia-coli-in-common-vampire-bats-desmodus-rotundus-and-livestock-in-peru
#2
J A Benavides, C Shiva, M Virhuez, C Tello, A Appelgren, J Vendrell, J Solassol, S Godreuil, D G Streicker
Antibiotic resistance mediated by bacterial production of extended-spectrum beta-lactamase (ESBL) is a global threat to public health. ESBL resistance is most commonly hospital-acquired; however, infections acquired outside of hospital settings have raised concerns over the role of livestock and wildlife in the zoonotic spread of ESBL-producing bacteria. Only limited data are available on the circulation of ESBL-producing bacteria in animals. Here, we report ESBL-producing Escherichia coli in wild common vampire bats Desmodus rotundus and livestock near Lima, Peru...
March 25, 2018: Zoonoses and Public Health
https://www.readbyqxmd.com/read/29565231/genomic-identity-of-fluoroquinolone-resistant-blactx-m-15-type-esbl-and-pmampc-%C3%AE-lactamase-producing-klebsiella-pneumoniae-from-buffalo-milk-india
#3
Samiran Bandyopadhyay, Jaydeep Banerjee, Debaraj Bhattacharyya, Indranil Samanta, Achintya Mahanti, Tapan K Dutta, Sarbaswarup Ghosh, Pramod K Nanda, Premanshu Dandapat, Subhasish Bandyopadhyay
We investigated the occurrence of extended-spectrum β-lactamase (ESBL) and AmpC-type β-lactamase (ACBL) producing quinolone-resistant Klebsiella pneumoniae (KP) in milk samples of apparently healthy buffaloes (n = 348) and buffaloes (n = 19) with evidence of subclinical mastitis from seven districts of West Bengal, India. In total, 12 ESBL producing KP were isolated with blaCTX-M-15 gene and 7 of them were ACBL producers, as well. The blaCTX-M-15 genes were carried by transposable element ISEcp1. The plasmid-mediated quinolone resistance genes-qnrS, qnrA, qnrB, qepA, and aac(6')-Ib-cr were detected in five, one, three, four, and one isolate (s), respectively...
March 22, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29550142/travel-and-acquisition-of-multidrug-resistant-enterobacteriaceae
#4
REVIEW
L Armand-Lefèvre, A Andremont, E Ruppé
Bacterial resistance to antibiotics is considered a major threat to health. Enterobacteriaceae have increasingly become resistant to antibiotics through the acquisition and dissemination of extended-spectrum beta-lactamases (ESBL) that confer resistance to most beta-lactams. While ESBL-producing Enterobacteriaceae were formerly restricted to hospitals, they have now spread to community settings, especially in developing countries. The tremendous expansion of international travels contributed to the importation of multidrug-resistant Enterobacteriaceae (MRE) to low prevalence countries...
March 14, 2018: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/29503800/prevalence-of-fecal-carriage-of-ctx-m-15-beta-lactamase-producing-escherichia-coli-in-healthy-children-from-a-rural-andean-village-in-venezuela
#5
María Araque, Indira Labrador
Objectives: Antimicrobial resistant extended-spectrum-β-lactamase-producing Enterobacteriaceae (ESBL-PE) have been shown to be present in healthy communities. This study examined healthy children from the rural Andean village of Llano del Hato, Mérida, Venezuela, who have had little or no antibiotic exposure to determine the prevalence of fecal carriage of ESBL-producing Escherichia coli (ESBL-EC). Methods: A total of 78 fecal samples were collected in healthy children aged from 1 to 5 years...
January 2018: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/29503415/-antibiotic-residue-in-environmental-water-in-vietnam
#6
Kazuo Harada
 The increasing prevalence of antimicrobial resistance (AMR) has caused intractable infections worldwide. Nearly 50% of the healthy population of Southeast Asia carries extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. The overuse of antimicrobial agents in the agriculture, aquaculture, and medical care sectors causes environmental pollution, facilitating the spread of AMR. However, there is a lack of data pertaining to antimicrobial residues in environmental water in such regions. We investigated a total of 49 chemicals, including β-lactams, sulfonamides, quinolones, and tetracyclines...
2018: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/29421359/emergence-of-plasmid-mediated-colistin-resistance-in-cmy-2-producing-escherichia-coli-of-lineage-st2197-in-a-tunisian-poultry-farm
#7
Elaa Maamar, Carla Andrea Alonso, Zaineb Hamzaoui, Nouha Dakhli, Mohamed Salah Abbassi, Sana Ferjani, Mabrouka Saidani, Ilhem Boutiba-Ben Boubaker, Carmen Torres
Our study aimed to investigate colistin resistance and the mechanisms involved in a collection of 35 extended-spectrum beta-lactamase (ESBL) and 13 CMY-2-producing E. coli strains which were previously recovered from chicken gut microbiota in Tunisia, as well as to determine the genetic location of mcr genes. Forty-eight ESBL and CMY-2-producing E. coli strains were obtained from 137 fecal samples of healthy chickens during 2013. These strains were tested for colistin resistance by the broth microdilution method, and screened for mcr-1 and mcr-2 genes by PCR...
January 29, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29230684/imipenem-relebactam-and-meropenem-vaborbactam-two-novel-carbapenem-%C3%AE-lactamase-inhibitor-combinations
#8
REVIEW
George G Zhanel, Courtney K Lawrence, Heather Adam, Frank Schweizer, Sheryl Zelenitsky, Michael Zhanel, Philippe R S Lagacé-Wiens, Andrew Walkty, Andrew Denisuik, Alyssa Golden, Alfred S Gin, Daryl J Hoban, Joseph P Lynch, James A Karlowsky
Relebactam (formerly known as MK-7655) is a non-β-lactam, bicyclic diazabicyclooctane, β-lactamase inhibitor that is structurally related to avibactam, differing by the addition of a piperidine ring to the 2-position carbonyl group. Vaborbactam (formerly known as RPX7009) is a non-β-lactam, cyclic, boronic acid-based, β-lactamase inhibitor. The structure of vaborbactam is unlike any other currently marketed β-lactamase inhibitor. Both inhibitors display activity against Ambler class A [including extended-spectrum β-lactamases (ESBLs), Klebsiella pneumoniae carbapenemases (KPCs)] and class C β-lactamases (AmpC)...
January 2018: Drugs
https://www.readbyqxmd.com/read/29155322/digestive-tract-colonization-by-multidrug-resistant-enterobacteriaceae-in-travellers-an-update
#9
REVIEW
Etienne Ruppé, Antoine Andremont, Laurence Armand-Lefèvre
BACKGROUND: Enterobacteriaceae have become increasingly resistant, especially due to the acquisition and spread of extended-spectrum beta-lactamases (ESBLs), which confer resistance to the majority of beta-lactams. Multi-resistant Enterobacteriaceae (MRE) were first isolated in hospitals, but now they are disseminating in the community setting, mostly in low and middle income countries. Consequently, the increasing number of international travels leads to the importation of MRE from high-prevalence to low-prevalence countries...
November 17, 2017: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/29067059/phenotypic-characterization-of-extended-spectrum-beta-lactamase-producing-e-coli-from-healthy-individuals-patients-sewage-sludge-cattle-chickens-and-raw-meat
#10
Rabia Saleem, Hasan Ejaz, Aizza Zafar, Sonia Younas, Ahsan Waheed Rathore
OBJECTIVE: The present study aimed to determine the frequency and antimicrobial profile of ESBL-producing isolates of E. coli in different environments. METHODS: This cross-sectional study was conducted at The Children's Hospital and The Institute of Child Health, Lahore from July to December 2015. The faecal specimens from healthy individuals, patients, sewage sludge, cattle, chickens and raw meat (n = 122) were processed for microbiological analysis using MacConkey agar supplemented with cefotaxime...
July 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/29051750/epidemiology-of-extended-spectrum-%C3%AE-lactamase-producing-e-coli-and-vancomycin-resistant-enterococci-in-the-northern-dutch-german-cross-border-region
#11
Xuewei Zhou, Silvia García-Cobos, Gijs J H M Ruijs, Greetje A Kampinga, Jan P Arends, Dirk M Borst, Lieke V Möller, Nicole D Holman, Theo A Schuurs, Lesla E Bruijnesteijn van Coppenraet, Jan F Weel, Jan H van Zeijl, Robin Köck, John W A Rossen, Alexander W Friedrich
Objectives: To reveal the prevalence and epidemiology of extended-spectrum β-lactamase (ESBL)- and/or plasmid AmpC (pAmpC)- and carbapenemase (CP) producing Enterobacteriaceae and vancomycin-resistant enterococci (VRE) across the Northern Dutch-German border region. Methods: A point-prevalence study on ESBL/pAmpC/CP producing Enterobacteriaceae and VRE was carried out in hospitalized patients in the Northern Netherlands ( n = 445, 2012-2013) and Germany ( n = 242, 2012). Healthy individuals from the Dutch community ( n = 400, 2010-2012) were also screened...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29042339/clonal-dissemination-of-oxa-48-producing-enterobacter-cloacae-isolated-from-companion-animals-in-algeria
#12
Massilia Yousfi, Abdelaziz Touati, Anaelle Muggeo, Brazane Mira, Asma Bourouis, Lucien Brasme, Thomas Guillard, Christophe de Champs
OBJECTIVES: The aim of this study was to investigate carbapenemase-producing Enterobacteriaceae (CPE) in companion animals. METHODS: Between October 2015 and April 2016, 533 rectal swabs were obtained from healthy and diseased pets in different cities in Algeria. Samples collected were plated on MacConkey agar supplemented with ertapenem (0.5mg/L). The isolates were identified by MALDI-TOF mass spectroscopy. Antimicrobial susceptibility testing was performed using disk diffusion method...
October 14, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29031106/new-insights-into-resistance-to-colistin-and-third-generation-cephalosporins-of-escherichia-coli-in-poultry-portugal-novel-blactx-m-166-and-blaesac-genes
#13
Vera Manageiro, Lurdes Clemente, Rafael Graça, Ivone Correia, Teresa Albuquerque, Eugénia Ferreira, Manuela Caniça
The increasing incidence of intestinal colonization with extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae and Gram negative organisms that has been observed in food animals such as poultry, cattle and pigs, are suggestive that animals, food and environment are potential sources of ESBL-producing bacteria. Hence, the aim of this study was to characterized commensal E. coli obtained from healthy broiler and turkey flocks at slaughter for the presence of penicillinases-, ESBL-, extended-spectrum AmpC (ESAC)-, plasmid-mediated quinolone resistance- and MCR-encoding genes...
October 4, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28992133/intestinal-carriage-of-third-generation-cephalosporin-resistant-and-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-in-healthy-us-children
#14
Shamim Islam, Rangaraj Selvarangan, Neena Kanwar, Rendie McHenry, James D Chappell, Natasha Halasa, Mary E Wikswo, Daniel C Payne, Parvin H Azimi, L Clifford McDonald, Oscar G Gomez-Duarte
Background: The epidemiology of antibiotic-resistant Enterobacteriaceae intestinal carriage in healthy US children has not been well characterized. Methods: Children between 14 days and 14 years of age were enrolled during well-child visits in Oakland, California, Kansas City, Kansas, and Nashville, Tennessee, between December 2013 and March 2015. Data on recent antibiotic use by the child and travel and hospitalization history of all members of each child's household were obtained with a risk-factor survey...
June 15, 2017: Journal of the Pediatric Infectious Diseases Society
https://www.readbyqxmd.com/read/28939488/prevalence-and-transmission-dynamics-of-escherichia-coli-st131-among-contacts-of-infected-community-and-hospitalized-patients
#15
E Torres, L López-Cerero, I Morales, M D Navarro, J Rodríguez-Baño, A Pascual
OBJECTIVES: The Escherichia coli O25b-associated ST131 clonal group was recently found to be prevalent in our area as a cause of community-acquired urinary tract infections. We evaluated the transmission dynamics and longitudinal persistence of E. coli O25b-ST131 between patients with nosocomial and community-acquired infections and their contacts. METHODS: Prevalence and transmission of O25b/pabB3/B23 isolates were compared in 38 community clusters, 30 nosocomial clusters and 50 healthy volunteers...
September 19, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28921905/antibiotic-resistant-escherichia-coli-in-southeastern-australian-pig-herds-and-implications-for-surveillance
#16
L K van Breda, O P Dhungyel, M P Ward
To investigate public health implications of antibiotics to control post-weaning scours, we surveyed 22 commercial pig herds in southeastern Australia. Fifty faecal samples per herd were collected from pre- and post-weaned piglets. Presumptive Escherichia coli isolates were confirmed by MALDI-TOF MS. Isolates (n = 325) were screened for susceptibility to 19 veterinary antibiotics using MIC broth microdilution. All 325 E. coli isolates underwent further testing against 27 antibiotics used in human medicine and were screened for ETEC adhesin and enterotoxin genes (F4 (K88), F5 (K99), F6 (987P), F18, F41, STa, STb, Stx2e and LT) by multiplex PCR...
February 2018: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28919689/beta-lactamase-antimicrobial-resistance-in-klebsiella-and-enterobacter-species-isolated-from-healthy-and-diarrheic-dogs-in-andhra-pradesh-india
#17
N Mohammad Sharif, B Sreedevi, R K Chaitanya, D Sreenivasulu
AIM: The aim of this study was to characterize beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh. MATERIALS AND METHODS: A total of 136 rectal swabs were collected from healthy (92) and diarrheic (44) dogs, bacteriological cultured for Klebsiella and Enterobacter growth and screened for beta-lactamase antimicrobial resistance phenotypically by disc diffusion method and genotypically by polymerase chain reaction targeting blaTEM, blaSHV, blaOXA, blaCTX-M Group 1, 2, blaAmpC, blaACC, and blaMOX genes...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28918585/prevalence-and-risk-factors-for-intestinal-carriage-of-ctx-m-type-esbls-in-enterobacteriaceae-from-a-thai-community
#18
P R Niumsup, U Tansawai, A Na-Udom, D Jantapalaboon, K Assawatheptawee, A Kiddee, T Romgaew, S Lamlertthon, T R Walsh
The incidence of infections caused by antimicrobial-resistant Enterobacteriaceae in Thailand is increasing and human intestinal flora is an important reservoir for these organisms. This study was carried out to determine the intestinal carriage of blaCTX-M extended spectrum ß-lactamase-positive Enterobacteriaceae (ESBL + E) and AmpC-positive Enterobacteriaceae in a community setting in Northern Thailand, and to identify potential risk factors for carriage. A total of 307 fecal samples were collected from healthy volunteers in Phitsanulok province, and cefotaxime-resistant Enterobacteriaceae (CtxRE) were isolated using selective media...
January 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28898269/faecal-carriage-of-ctx-m-extended-spectrum-beta-lactamase-producing-enterobacteriaceae-among-street-children-dwelling-in-mwanza-city-tanzania
#19
Nyambura Moremi, Heike Claus, Ulrich Vogel, Stephen E Mshana
BACKGROUND: Data on ESBL carriage of healthy people including children are scarce especially in developing countries. We analyzed the prevalence and genotypes of ESBL-producing Enterobacteriaceae (EPE) in Tanzanian street children with rare contact to healthcare facilities but significant interactions with the environment, animals and other people. METHODOLOGY/ PRINCIPLE FINDINGS: Between April and July 2015, stool samples of 107 street children, who live in urban Mwanza were analyzed for EPE...
2017: PloS One
https://www.readbyqxmd.com/read/28861076/case-of-meningitis-in-a-neonate-caused-by-an-extended-spectrum-beta-lactamase-producing-strain-of-hypervirulent-klebsiella-pneumoniae
#20
Khalit S Khaertynov, Vladimir A Anokhin, Yuri N Davidyuk, Irina V Nicolaeva, Svetlana V Khalioullina, Dina R Semyenova, Evgeny Y Alatyrev, Natalia N Skvortsova, Levon G Abrahamyan
Klebsiella pneumoniae is one of the most important infectious agents among neonates. This pathogen has a potential to develop an increased antimicrobial resistance and virulence. The classic non-virulent strain of K. pneumoniae, producing an extended-spectrum beta-lactamases (ESBL), is associated with nosocomial infection mainly in preterm neonates. Hypervirulent K. pneumoniae strains are associated with invasive infection among previously healthy ambulatory patients, and most of them exhibit antimicrobial susceptibility...
2017: Frontiers in Microbiology
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