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https://www.readbyqxmd.com/read/28483306/pharmacodynamic-activity-of-fosfomycin-simulating-urinary-concentrations-achieved-after-a-single-3-g-oral-dose-versus-escherichia-coli-using-an-in-vitro-model
#1
George G Zhanel, Kate Parkinson, Sean Higgins, Andrew Denisuik, Heather Adam, Johann Pitout, Ayman Noreddin, James A Karlowsky
We assessed the activity of fosfomycin simulating urinary concentrations achieved after a single 3-g oral dose against Escherichia coli using an in vitro pharmacodynamic model. Eleven urinary isolates of E. coli were studied. Isolates were ESBL-producing or carbapenemase-producing. The in vitro pharmacodynamic model was inoculated with an inoculum of (~1×10(6)cfu/mL). Fosfomycin was administered to simulate maximum free (ƒ) urine (U) concentrations and a t1/2 obtained after a standard single 3-g oral dose in healthy volunteers (ƒUmax, 4000mg/L; t1/2, 6h)...
April 23, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28405193/the-efficacy-of-isolated-bacteriophages-from-pig-farms-against-esbl-ampc-producing-escherichia-coli-from-pig-and-turkey-farms
#2
Aneta Skaradzińska, Paulina Śliwka, Marta Kuźmińska-Bajor, Grzegorz Skaradziński, Anna Rząsa, Anika Friese, Nicole Roschanski, Jayaseelan Murugaiyan, Uwe H Roesler
Extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases are plasmid (but also chromosomally) encoded enzymes found in Enterobacteriaceae, determining resistance to a variety of important antibiotics including penicillins, cephalosporins, and monobactams. In recent decades, the prevalence of ESBL/AmpC-producing bacteria has increased rapidly across the world. Here, we evaluate the potential use of bacteriophages in terms of a reduction of antibiotic-resistant bacteria in healthy animals. The aim of our studies was to isolate bacteriophages capable of destroying ESBL/AmpC-producing Escherichia coli isolated from livestock habitats...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28359312/extended-spectrum-beta-lactamase-producing-pseudomonas-aeruginosa-in-camel-in-egypt-potential-human-hazard
#3
Mahmoud Elhariri, Dalia Hamza, Rehab Elhelw, Sohad M Dorgham
BACKGROUND: The rapid increase of extended-spectrum beta-lactamase (ESBL) producing bacteria are a potential health hazard. Development of antimicrobial resistance in animal pathogens has serious implications for human health, especially when such strains could be transmitted to human. In this study, the antimicrobial resistance due to ESBL producing Pseudomonas aeruginosa in the camel meat was investigated. METHODS: In this study meat samples from 200 healthy camels at two major abattoirs in Egypt (Cairo and Giza) were collected...
March 31, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28314728/competitive-exclusion-reduces-transmission-and-excretion-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-in-broilers
#4
Daniela Ceccarelli, Alieda van Essen-Zandbergen, Bregtje Smid, Kees T Veldman, Gert Jan Boender, Egil A J Fischer, Dik J Mevius, Jeanet A van der Goot
Extended-spectrum β-lactamases (ESBLs) and plasmid mediated AmpC β-lactamases (pAmpC) are enzymes able to hydrolyze a large variety of β-lactam antibiotics, including 3(rd) generation cephalosporins and monobactams. Broilers and broiler meat products can be highly contaminated with ESBL and pAmpC-producing Escherichia coli, also known as extended-spectrum cephalosporins (ESC)-resistant E. coli, and can be a source for human infections. As few data are available on interventions to reduce ESC-resistant E...
March 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28258755/unexpected-common-occurrence-of-transferable-extended-spectrum-cephalosporinase-producing-escherichia-coli-in-swedish-surface-waters-used-for-drinking-water-supply
#5
Maria Egervärn, Stina Englund, Marianne Ljunge, Christer Wiberg, Maria Finn, Mats Lindblad, Stefan Börjesson
The presence of Enterobacteriaceae producing extended spectrum beta-lactamases (ESBL) or transferable AmpC beta-lactamases (pAmpC) is increasingly being reported in humans, food-producing animals and food world-wide. However, the occurrence and impact of these so-called extended spectrum cephalosporinase (ESC)-producing Enterobacteriaceae in aquatic environments are poorly documented. This study investigated the occurrence, concentrations and characteristics of ESC-producing E. coli (ESC-Ec) in samples of surface water collected at five Swedish water treatment plants that normally have relatively high prevalence and concentration of E...
February 28, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28256291/comparative-analysis-of-the-susceptibility-to-biocides-and-heavy-metals-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolates-of-human-and-avian-origin-germany
#6
Daniela Deus, Carsten Krischek, Yvonne Pfeifer, Ahmad Reza Sharifi, Ulrike Fiegen, Felix Reich, Guenter Klein, Corinna Kehrenberg
A total of 174 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates collected from humans (n=140) and healthy broiler chickens (n = 34) was included in the study. The MIC values of alkyl diaminoethyl glycin hydrochloride, benzethonium chloride, benzalkonium chloride, chlorhexidine, acriflavine, copper sulfate, silver nitrate and zinc chloride were determined by the broth microdilution method. Significant differences in MIC distributions were found between human and avian isolates and between CTX-M-, SHV- and TEM-type ESBL E...
May 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28220620/prevalence-of-ampc-and-extended-spectrum-%C3%AE-lactamase-harbouring-enterobacteriaceae-in-faecal-flora-of-a-healthy-domestic-canine-population
#7
D A Mathys, D F Mollenkopf, C A Bremer, J B Daniels, T E Wittum
In order to estimate the prevalence of AmpC- and ESBL β-lactamase-producing Enterobacteriaceae in the faecal flora of a healthy domestic canine population, faecal samples were obtained from healthy dogs receiving routine parasitology screening at the Ohio State University Veterinary Medical Center, between January 2013 and April 2013. Samples were screened for the presence of AmpC and ESBL β-lactamase phenotypes, and the clinically important genotypes, blaCMY and blaCTX-M , were confirmed via conventional PCR...
February 20, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28167688/implant-associated-esbl-klebsiella-pneumonia-producing-small-colony-variant-bone-and-joint-infection-in-a-healthy-40-year-old-man
#8
Cécile Ronde-Oustau, Sébastien Lustig, Céline Dupieux, Tristan Ferry
No abstract text is available yet for this article.
February 6, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28135647/spread-of-esbl-ampc-producing-escherichia-coli-and-klebsiella-pneumoniae-in-the-community-through-ready-to-eat-sandwiches-in-algeria
#9
Lydia Yaici, Marisa Haenni, Véronique Métayer, Estelle Saras, Ferielle Mesbah Zekar, Meriem Ayad, Abdelaziz Touati, Jean-Yves Madec
The spread of Extended-Spectrum β-Lactamases (ESBLs) or AmpC β-Lactamases (AmpC) encoding genes in healthy human populations is of major concern. The role of the food chain has been questioned since numerous studies reported surface contamination of retail meat or crude vegetables with ESBL/AmpC-producing Enterobacteriaceae (ESBL/AmpC-E). Nonetheless, these food products are intended to be cooked or washed before consumption so that the risk of human transfer might be low. Here, the presence of ESBL/AmpC-E was investigated in ready-to-eat (RTE) sandwiches purchased in the street in the city of Bejaia, Algeria...
March 20, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28123141/antimicrobial-resistance-profiles-and-molecular-characterization-of-escherichia-coli-strains-isolated-from-healthy-adults-in-ho-chi-minh-city-vietnam
#10
Phuong Hoai Hoang, Sharda Prasad Awasthi, Phuc DO Nguyen, Ngan Ly Hoang Nguyen, Dao Thi Anh Nguyen, Ninh Hoang LE, Chinh VAN Dang, Atsushi Hinenoya, Shinji Yamasaki
In this study, we attempted to isolate Escherichia coli from healthy adults in Ho Chi Minh City, Vietnam, and characterized its antimicrobial resistance profile, extended-spectrum β-lactamase (ESBL) genotype, phylogenetic grouping and virulence gene profile. A total of 103 E. coli isolates were obtained, and most of them were antimicrobial resistant such to streptomycin (80.6%), tetracycline (67.0%), ampicillin (65.0%), sulfamethoxsazole/trimethoprim (48.5%), nalidixic acid (43.7%), chloramphenicol (34.0%), cefotaxime (15...
March 18, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28049493/presence-of-foodborne-pathogens-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-and-methicillin-resistant-staphylococcus-aureus-in-slaughtered-reindeer-in-northern-finland-and-norway
#11
Sauli Laaksonen, Antti Oksanen, Jérôme Julmi, Claudio Zweifel, Maria Fredriksson-Ahomaa, Roger Stephan
BACKGROUND: Various food-producing animals were recognized in recent years as healthy carriers of bacterial pathogens causing human illness. In northern Fennoscandia, the husbandry of semi-domesticated reindeer (Rangifer tarandus tarandus) is a traditional livelihood and meat is the main product. This study determined the presence of selected foodborne pathogens, methicillin-resistant Staphylococcus aureus (MRSA), and extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in healthy semi-domesticated reindeer at slaughter in northern Finland and Norway...
January 3, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28018582/carriage-of-esbl-ampc-producing-or-ciprofloxacin-non-susceptible-escherichia-coli-and-klebsiella-spp-in-healthy-people-in-norway
#12
Charlotte R Ulstad, Margrete Solheim, Sophie Berg, Morten Lindbæk, Ulf R Dahle, Astrid L Wester
BACKGROUND: Asymptomatic carriage has been recognised as an important risk factor for infection caused by antibiotic resistant bacteria. A 14% global prevalence of Extended-Spectrum Beta-lactamase (ESBL) carriage was recently reported, but large intra-and interregional variations were observed. We investigated the faecal carriage rates of ESBL-, AmpC-producing and ciprofloxacin non-susceptible Escherichia coli and Klebsiella spp. in healthy Norwegians. METHODS: Rectal samples were obtained from 284 volunteers, together with demographic data and information on recent travel history...
2016: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/27956778/study-of-antimicrobial-resistance-due-to-extended-spectrum-beta-lactamase-producing-escherichia-coli-in-healthy-broilers-of-jabalpur
#13
Arpita Shrivastav, R K Sharma, Y P Sahni, Neeraj Shrivastav, Vidhi Gautam, Sachin Jain
AIM: To study the prevalence of antimicrobial resistance due to extended spectrum beta-lactamase (ESBL)-producing Escherichia coli in samples collected from the ceca of healthy broilers of poultry sale outlets (PSOs) Jabalpur. MATERIALS AND METHODS: A total of 400 cecal swab samples were taken randomly from freshly slaughtered poultry of 39 PSOs located at four different zones or areas of Jabalpur and were screened for the presence of ESBL-producing E. coli using standard methods...
November 2016: Veterinary World
https://www.readbyqxmd.com/read/27936054/high-prevalence-of-faecal-carriage-of-esbl-producing-enterobacteriaceae-among-children-in-dar-es-salaam-tanzania
#14
Marit G Tellevik, Bjørn Blomberg, Øyvind Kommedal, Samuel Y Maselle, Nina Langeland, Sabrina J Moyo
BACKGROUND: Faecal carriage of ESBL-producing bacteria is a potential risk for transmission and infection. Little is known about faecal carriage of antibiotic resistance in Tanzania. This study aimed to investigate the prevalence of faecal carriage of ESBL-producing Enterobacteriaceae and to identify risk factors for carriage among young children in Tanzania. METHODOLOGY/PRINCIPAL FINDINGS: From August 2010 to July 2011, children below 2 years of age were recruited in Dar es Salaam, including healthy community children (n = 250) and children hospitalized due to diarrhoea (n = 250) or other diseases (n = 103)...
2016: PloS One
https://www.readbyqxmd.com/read/27872719/phylogenetic-typing-and-detection-of-extended-spectrum-%C3%AE-lactamases-in-escherichia-coli-isolates-from-broiler-chickens-in-ahvaz-iran
#15
Ramezan Ali Jafari, Hossein Motamedi, Elham Maleki, Reza Ghanbarpour, Mansoor Mayahi
This study was conducted to reveal the phylogenetic background, to detect the genes encoding TEM, SHV and CTX-M-15 extended-spectrum β-lactamases (ESBL), and to analyze their distribution in phylo-groups of 150 Escherichia coli isolates from broiler chickens in Ahvaz (Southwest of Iran). Seventy- five cloacal swabs from healthy birds (fecal isolates), and 75 heart blood samples from birds with colibacillosis (septicemic isolates) were obtained. All isolates were phylotyped and screened for ESBL genes by polymerase chain reaction (PCR)...
2016: Veterinary Research Forum
https://www.readbyqxmd.com/read/27856044/community-fecal-carriage-of-broad-spectrum-cephalosporin-resistant-escherichia-coli-in-tunisian-children
#16
Sana Ferjani, Mabrouka Saidani, Zeineb Hamzaoui, Carla Andrea Alonso, Carmen Torres, Elaa Maamar, Amine Faouzi Slim, Ben Boubaker Ilhem Boutiba
The spread of extended spectrum β-lactamases (ESBL) and plasmid mediated AmpC β-lactamases (pAmpC) was evaluated in Escherichia coli strains collected from the intestinal microbiota of healthy children in Tunisia. The carriage rate of CTX(R)E. coli was 6.6% (7 of 105 samples) and one strain/sample was further characterized (7 isolates). These isolates harbored blaCTX-M-1 (n = 4), blaCTX-M-15 (n = 2), and blaCMY-2 gene (n = 1), which were usually located on FIB replicon type and carried class 1 integrons. The acc(6')-Ib-cr variant was identified in one isolate that harbored blaCTX-M-15...
February 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27798205/community-carriage-of-esbl-producing-escherichia-coli-is-associated-with-strains-of-low-pathogenicity-a-swedish-nationwide-study
#17
Sofia Ny, Sonja Löfmark, Stefan Börjesson, Stina Englund, Maj Ringman, Jakob Bergström, Pontus Nauclér, Christian G Giske, Sara Byfors
OBJECTIVES: Community carriage of ESBL-producing Escherichia coli (EPE) is common worldwide and there is a need to understand the connection between carriage and infection. We compared the molecular characteristics of EPE among Swedish community carriers with those of EPE causing invasive infections. METHODS: We collected 2134 faecal samples from randomly selected Swedish inhabitants and examined them for the presence of EPE. All participating volunteers answered a questionnaire about putative risk factors for EPE carriage...
October 24, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27712137/an-update-on-the-management-of-urinary-tract-infections-in-the-era-of-antimicrobial-resistance
#18
REVIEW
Mazen S Bader, Mark Loeb, Annie A Brooks
Urinary tract infections (UTIs) caused by antibiotic-resistant Gram-negative bacteria are a growing concern due to limited therapeutic options. Gram-negative bacteria, specifically Enterobacteriaceae, are common causes of both community-acquired and hospital acquired UTIs. These organisms can acquire genes that encode for multiple antibiotic resistance mechanisms, including extended-spectrum-lactamases (ESBLs), AmpC- β -lactamase, and carbapenemases. The assessment of suspected UTI includes identification of characteristic symptoms or signs, urinalysis, dipstick or microscopic tests, and urine culture if indicated...
March 2017: Postgraduate Medicine
https://www.readbyqxmd.com/read/27694727/multidrug-resistant-esbl-producing-enterobacteriaceae-and-associated-risk-factors-in-community-infants-in-lebanon
#19
Soumaya Moustafa Hijazi, Mohamad Anwar Fawzi, Faten Moustafa Ali, Khaled Hussein Abd El Galil
INTRODUCTION: Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) infections are a growing threat to children, and the treatment of these infections becomes more and more challenging. A huge reservoir for ESBLs in the community is the fecal flora of children. This study investigates the rectal colonization, associated risk factors, antimicrobial susceptibility, and molecular characterization of ESBL-PE in Lebanese community infants. METHODOLOGY: A total of 117 rectal swabs were taken from healthy infants between 1 and 12 months of age...
September 30, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/27681363/prevalence-and-quinolone-resistance-of-fecal-carriage-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-in-6-communities-and-2-physical-examination-center-populations-in-shanghai-china
#20
Qi Ni, Yuan Tian, Lihua Zhang, Cen Jiang, Danfeng Dong, Zhen Li, Enqiang Mao, Yibing Peng
OBJECTIVES: To characterize extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates from the community, determine their antibiotic sensitivity profiles and quinolone resistance mechanisms, and identify any horizontal transfer of ESBL genes. METHODS: One thousand seven hundred thirty-two stool samples were collected from healthy individuals in 6 communities and 2 physical examination centers in Shanghai, China. ESBL-producing E. coli was screened and confirmed by confirmatory test and E...
December 2016: Diagnostic Microbiology and Infectious Disease
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