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https://www.readbyqxmd.com/read/28921905/antibiotic-resistant-escherichia-coli-in-southeastern-australian-pig-herds-and-implications-for-surveillance
#1
L K van Breda, O P Dhungyel, M P Ward
To investigate public health implications of antibiotics to control post-weaning scours, we surveyed 22 commercial pig herds in southeastern Australia. Fifty faecal samples per herd were collected from pre- and post-weaned piglets. Presumptive Escherichia coli isolates were confirmed by MALDI-TOF MS. Isolates (n = 325) were screened for susceptibility to 19 veterinary antibiotics using MIC broth microdilution. All 325 E. coli isolates underwent further testing against 27 antibiotics used in human medicine and were screened for ETEC adhesin and enterotoxin genes (F4 (K88), F5 (K99), F6 (987P), F18, F41, STa, STb, Stx2e and LT) by multiplex PCR...
September 17, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28919689/beta-lactamase-antimicrobial-resistance-in-klebsiella-and-enterobacter-species-isolated-from-healthy-and-diarrheic-dogs-in-andhra-pradesh-india
#2
N Mohammad Sharif, B Sreedevi, R K Chaitanya, D Sreenivasulu
AIM: The aim of this study was to characterize beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh. MATERIALS AND METHODS: A total of 136 rectal swabs were collected from healthy (92) and diarrheic (44) dogs, bacteriological cultured for Klebsiella and Enterobacter growth and screened for beta-lactamase antimicrobial resistance phenotypically by disc diffusion method and genotypically by polymerase chain reaction targeting blaTEM, blaSHV, blaOXA, blaCTX-M Group 1, 2, blaAmpC, blaACC, and blaMOX genes...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28918585/prevalence-and-risk-factors-for-intestinal-carriage-of-ctx-m-type-esbls-in-enterobacteriaceae-from-a-thai-community
#3
P R Niumsup, U Tansawai, A Na-Udom, D Jantapalaboon, K Assawatheptawee, A Kiddee, T Romgaew, S Lamlertthon, T R Walsh
The incidence of infections caused by antimicrobial-resistant Enterobacteriaceae in Thailand is increasing and human intestinal flora is an important reservoir for these organisms. This study was carried out to determine the intestinal carriage of bla CTX-M extended spectrum ß-lactamase-positive Enterobacteriaceae (ESBL + E) and AmpC-positive Enterobacteriaceae in a community setting in Northern Thailand, and to identify potential risk factors for carriage. A total of 307 fecal samples were collected from healthy volunteers in Phitsanulok province, and cefotaxime-resistant Enterobacteriaceae (CtxRE) were isolated using selective media...
September 16, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28918351/susceptibility-profiles-of-bacteria-causing-urinary-tract-infection-in-southern-tunisia
#4
Sonda Guermazi-Toumi, Sirine Boujlel, Mouna Assoudi, Riadh Issaoui, Sonia Tlili, Mohamed Ennaceur Hlaiem
OBJECTIVES: The aim of this study was to determine the epidemiological profile and the antibiotic resistance of bacteria responsible for urinary infections diagnosed in the department of microbiology of the regional hospital Houcine Bouzaiene (Gafsa) in the south west of Tunisia. METHODS: All the cytobacteriological urine samples analyzed from 1 January 2015 to 30 June 2016 were included in the study. The adopted criteria to define urinary infection were: leukocyturia >10(4)/ml and bacteriuria >10(5)CFU/ml...
September 13, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28910943/-study-on-antibiotic-resistance-of-escherichia-coli-and-enterococcus-colonized-in-intestine-of-neonates-from-neonatal-intensive-care-unit
#5
X F Li, Z J Liu, X Chen, J Li, Z G Cui, B Kan, J R Ma, J H Cui
Objective: To understand the antibiotic resistance of bacteria colonized in intestine of the neonates from neonatal intensive care unit (NICU) and provide evidence to guide clinical antibiotic treatment. Methods: From May, 2014 to May, 2015, a total of 572 stool samples were collected from the neonates of NICU in our hospital. Escherichia coli and Enterococcus were detected with VITEK-2 system. Results: A total of 328 strains of E. coli and 243 strains of Enterococcus were isolated respectively in this study...
September 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28906528/real-time-activity-assays-of-%C3%AE-lactamases-in-living-bacterial-cells-application-to-the-inhibition-of-antibiotic-resistant-e-coli-strains
#6
Ying Ge, Ya-Jun Zhou, Ke-Wu Yang, Yi-Lin Zhang, Yang Xiang, Yue-Juan Zhang
The emergence of antibiotic resistance caused by β-lactamases, including serine β-lactamases (SβLs) and metallo-β-lactamases (MβLs), is a global public health threat. L1, a B3 subclass MβL, hydrolyzes almost all of known β-lactam antibiotics. We report a simple and straightforward UV-Vis approach for real-time activity assays of β-lactamases inside living bacterial cells, and this method has been exemplified by choosing antibiotics, L1 enzyme, Escherichia coli expressing L1 (L1 E. coli), Escherichia coli expressing extended-spectrum β-lactamases (ESBL-E...
September 14, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28906365/the-antibiotic-susceptibility-patterns-of-uropathogens-among-children-with-urinary-tract-infection-in-shiraz
#7
Gholamreza Pouladfar, Mitra Basiratnia, Mojtaba Anvarinejad, Pejman Abbasi, Fatemeh Amirmoezi, Samaneh Zare
Urinary tract infection (UTI) is one of the most common bacterial infections in pediatrics. Delay in diagnosis and treatment can cause significant morbidity. The physicians knowledge regarding the symptoms, microorganisms that caused UTI, and effective antibiotics in a geographical area can help them to select the appropriate antibiotics. This study was performed to determine the prevalence of bacteria that cause UTI and their susceptibility to common antibiotics as well as the common symptoms and associated factors in children of Shiraz, Southern Iran...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28904920/antimicrobial-susceptibility-pattern-of-extended-spectrum-beta-lactamases-producing-organisms-isolated-in-a-tertiary-care-hospital-bangladesh
#8
Mohammad Jobayer, Zeenat Afroz, Sultana Shazeda Nahar, Ayesha Begum, Shahin Ara Begum, S M Shamsuzzaman
CONTEXT: Infection caused by extended-spectrum beta-lactamases (ESBL) producing organism is a major problem regarding antibiotic resistance. AIMS: The aim of this study was to find out the antibiogram of ESBL producing organisms isolated from various samples. SETTINGS AND DESIGN: This cross-sectional study was carried out in the Department of Microbiology of a Tertiary Care Hospital, Dhaka, Bangladesh from January to June 2014. SUBJECTS AND METHODS: One Hundred and seventy-nine ESBL producing Gram-negative organisms detected phenotypically by double-disc synergy test were enrolled in this study...
July 2017: International Journal of Applied and Basic Medical Research
https://www.readbyqxmd.com/read/28904483/fecal-carriage-of-extended-spectrum-beta-lactamase-producing-enterobacteriaceae-in-intensive-care-unit-patients
#9
Shalini Shenoy Mulki, Kavya Ramamurthy, Sevitha Bhat
BACKGROUND AND AIMS: Increasing and indiscriminate use of antibiotics has led the bacteria to develop resistance to most of the antibiotics. Beta-lactamase production is the mechanism of resistance to beta-lactams. Extended-spectrum beta-lactamases (ESBLs) have been found in the members of Enterobacteriaceae such as Escherichia coli and Klebsiella spp. which are the common health-care-associated pathogens. The aim was to study the rate of fecal carriage of ESBL-producing Enterobacteriaceae in patients admitted to the Intensive Care Unit (ICU) of tertiary care hospital and follow them subsequently for the development of infections...
August 2017: Indian Journal of Critical Care Medicine
https://www.readbyqxmd.com/read/28904264/zoonotic-potential-and-antibiotic-resistance-of-escherichia-coli-in-neonatal-calves-in-uruguay
#10
Ana Umpiérrez, Inés Bado, Martín Oliver, Sofía Acquistapace, Analía Etcheverría, Nora Lía Padola, Rafael Vignoli, Pablo Zunino
Escherichia coli is one of the main etiological agents of neonatal calf diarrhea (NCD). The objective of this study was to assess the presence of virulence genes, genetic diversity, and antibiotic resistance mechanisms in E. coli associated with NCD in Uruguay. PCR was used to assess the presence of intimin, Shiga-like toxin, and stable and labile enterotoxin genes. Resistance to fluoroquinolones and oxyimino-cephalosporins was estimated on Müller-Hinton agar plates. Further antibiotic disc-diffusion tests were performed to assess bacterial multi-resistance...
September 12, 2017: Microbes and Environments
https://www.readbyqxmd.com/read/28901763/bioassay-guided-isolation-of-antibacterial-metabolites-from-emericella-sp-tj29
#11
Yan He, Zhengxi Hu, Qin Li, Jinfeng Huang, Xiao-Nian Li, Hucheng Zhu, Junjun Liu, Jianping Wang, Yongbo Xue, Yonghui Zhang
Bioassay-guided isolation of metabolites from cultures of the plant-derived fungus Emericella sp. TJ29 yielded three new terpene-polyketide hybrid meroterpenoids, emervaridones A-C (1-3), two new polyketides, varioxiranediols A and B (5 and 6), and three known analogues (4, 7, and 8). The structures and absolute configurations of these new compounds were elucidated by spectroscopic analyses, single-crystal X-ray diffraction, Mo2(OAc)4-induced electronic circular dichroism (ECD) data, and ECD calculations. To date, only one compound (4) bearing the emervaridone-type carbocyclic skeleton has been reported...
September 13, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28900355/distribution-of-blatem-blashv-and-blactx-m-genes-among-esbl-producing-p-aeruginosa-isolated-from-qazvin-and-tehran-hospitals-iran
#12
REVIEW
A Peymani, T Naserpour-Farivar, E Zare, K H Azarhoosh
INTRODUCTION: Pseudomonas aeruginosa is as an important opportunistic human pathogen, which is associated with several clinical infections that are usually difficult to treat because of resistance to multiple antimicrobials. The production of extendedspectrum ß-lactamases (ESBLs) is an important mechanism of ß-lactam resistance. The aims of this study were to determine the prevalence of ESBLs, antimicrobial susceptibility, and to detect the blaTEM, blaSHV, and blaCTX-M genes. METHODS: In this study, carried out from March 2013 to December 2014, 266 P...
June 2017: Journal of Preventive Medicine and Hygiene
https://www.readbyqxmd.com/read/28899399/a-comparison-of-blood-stream-infections-with-extended-spectrum-beta-lactamase-producing-and-non-producing-klebsiella-pneumoniae-in-pediatric-patients
#13
Sevgen Tanır Basaranoglu, Yasemin Ozsurekci, Kubra Aykac, Eda Karadag Oncel, Asiye Bıcakcigil, Banu Sancak, Ali Bulent Cengiz, Ates Kara, Mehmet Ceyhan
BACKGROUND: Rapid development and global spread of multidrug resistant Klebsiella pneumonia (K. pneumoniae) as a major cause of nosocomial infections is really remarkable. The aim of this study was to explore risk factors for health care associated blood stream infections (BSI) caused by ESBL-producing K. pneumoniae in children and analyze clinical outcomes. METHODS: A retrospective review of patients younger than 18 years-old with blood stream infection caused by K...
September 12, 2017: Italian Journal of Pediatrics
https://www.readbyqxmd.com/read/28898269/faecal-carriage-of-ctx-m-extended-spectrum-beta-lactamase-producing-enterobacteriaceae-among-street-children-dwelling-in-mwanza-city-tanzania
#14
Nyambura Moremi, Heike Claus, Ulrich Vogel, Stephen E Mshana
BACKGROUND: Data on ESBL carriage of healthy people including children are scarce especially in developing countries. We analyzed the prevalence and genotypes of ESBL-producing Enterobacteriaceae (EPE) in Tanzanian street children with rare contact to healthcare facilities but significant interactions with the environment, animals and other people. METHODOLOGY/ PRINCIPLE FINDINGS: Between April and July 2015, stool samples of 107 street children, who live in urban Mwanza were analyzed for EPE...
2017: PloS One
https://www.readbyqxmd.com/read/28893647/coexistence-of-multidrug-resistance-mechanisms-and-virulence-genes-in-carbapenem-resistant-pseudomonas-aeruginosa-strains-from-a-tertiary-care-hospital-south-india
#15
Kalaiarasan Ellappan, Harish Belgode Narasimha, Saravana Kumar
BACKGROUND: This study aimed to identify the multiple drug resistance mechanisms and various virulence genes in high level carbapenem resistant P. aeruginosa (CARPA) isolates collected from patients hospitalized in a tertiary care hospital, South India. METHODOLOGY: A total of 156 CARPA isolates were included in this study. Multiplex PCR was optimized to detect carbapenemases and extended-spectrum beta-lactamase (ESBL) genes, respectively. Semi-quantitative reverse transcriptase PCR (RT-PCR) was optimized to evaluate OprD deficiency...
September 8, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28893357/fluoroquinolone-resistance-in-extended-spectrum-%C3%AE-lactamase-producing-klebsiella-pneumoniae-in-a-japanese-tertiary-hospital-silent-shifting-to-ctx-m-15-producing-k-pneumoniae
#16
Masashi Higashino, Mika Murata, Yoshitomo Morinaga, Norihiko Akamatsu, Junichi Matsuda, Kazuaki Takeda, Norihito Kaku, Kosuke Kosai, Naoki Uno, Hiroo Hasegawa, Katsunori Yanagihara
PURPOSE: Fluoroquinolone resistance (FQ-r) in extended-spectrum β-lactamase (ESBL) producers is an urgent health concern in countries where ESBL-producing K. pneumoniae (ESBL-Kpn) is prevalent. We investigated FQ-r in Japan where ESBL-Kpn is less prevalent. METHODOLOGY: Clinical ESBL-Kpn isolates from 2011 to 2013 were collected in Nagasaki University Hospital. The ESBL genotypes included CTX-M-15, and the mechanisms of FQ-r through plasmid-mediated quinolone resistance (PMQR) and mutations in quinolone resistance-determining regions (QRDRs) were examined...
September 12, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28893195/antimicrobial-resistance-in-bacterial-pathogens-among-hospitalized-children-with-community-acquired-lower-respiratory-tract-infections-in-dongguan-china-2011-2016
#17
Xiaoguang He, Mingyu Xie, Siping Li, Junqin Ye, Qi Peng, Qiang Ma, Xiaomei Lu, Baimao Zhong
BACKGROUND: Bacterial pathogens are a major cause of childhood community acquired lower respiratory tract infections (CA-LRTIs), and few data described the impact of antimicrobial resistance on children with CA-LRTIs. This study aims to investigate the antimicrobial resistance in common bacterial agents among hospitalized children with CA-LRTIs between 2011 and 2016 in Dongguan, China. METHODS: Sputum samples were collected from hospitalized children (0-5 years old) with CA-LRTIs in Dongguan Children's Hospital...
September 11, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28893132/prediction-of-infection-caused-by-extended-spectrum-beta-lactamase-producing-enterobacteriaceae-development-of-a-clinical-decision-making-nomogram
#18
Ana García-Tello, Helena Gimbernat, Cristina Redondo, Elisa Meilán, David M Arana, Juana Cacho, Juan F Dorado, Javier C Angulo
OBJECTIVE: This study aimed to assess the population at risk of infection by extended-spectrum beta-lactamase (ESBL)-producing organisms, using clinical criteria. MATERIALS AND METHODS: All urine cultures positive for Enterobacteriaceae in a Spanish hospital department from January 2010 to 2014 were reviewed. All isolates with ESBL-positive strains were collected, and isolates received during the first week of each month with ESBL-negative strains from symptomatic patients hospitalized or admitted to the emergency room...
September 11, 2017: Scandinavian Journal of Urology
https://www.readbyqxmd.com/read/28892891/distribution-of-class-d-carbapenemase-and-extended-spectrum-%C3%AE-lactamase-genes-among-acinetobacter-baumannii-isolated-from-burn-wound-and-ventilator-associated-pneumonia-infections
#19
Maryam Mohammadi, Setareh Soroush, Somayeh Delfani, Iraj Pakzad, Abolfazl Abbaszadeh, Mahmoud Bahmani, Lidija Bogdanovic, Morovat Taherikalani
INTRODUCTION: Resistance to Acinetobacter baumannii is dramatically on the rise in Iran. Therefore, it is important to study resistance pattern among Acinetobacter isolates which is a common cause of nosocomial infections. AIM: To investigate antibiotic resistance patterns and the role of resistant genes and biofilm formation in the induction of resistance among Acinetobacter baumannii isolated from burn wound and ventilator associated pneumonia infections. MATERIALS AND METHODS: Total 103 isolates such as 33 burn samples from Rasool Akram Hospital and 70 isolates from ventilated patients in Shahid Motahhari Hospital were identified with A...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28890179/maternal-colonization-or-infection-with-extended-spectrum-beta-lactamase-producing-enterobacteriaceae-in-africa-a-systematic-review-and-meta-analysis
#20
Andre N H Bulabula, Angela Dramowski, Shaheen Mehtar
OBJECTIVES: To summarize published studies on the prevalence of and risk factors for maternal bacterial colonization and/or infection with extended-spectrum-beta-lactamases producing Enterobacteriaceae (ESBL-E) in pregnant and/or post-partum women in Africa. DESIGN OR METHODS: A systematic review was conducted in PubMed, Scopus and Google Scholar. Bibliographies of included eligible studies were manually searched to identify additional relevant articles. No language restriction was applied, for the timeframe, we searched from the electronic databases' inception to July 15th, 2017...
September 7, 2017: International Journal of Infectious Diseases: IJID
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