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Jiancheng Shu, Haiping Wu, Renlong Liu, Zuohua Liu, Bing Li, Mengjun Chen, Changyuan Tao
This study examined simultaneous stabilization and solidification (S/S) of Mn(2+) and NH4(+)-N from electrolytic manganese residue (EMR) using MgO and different phosphate resource. The characteristics of EMR NH4(+)-N and Mn(2+) S/S behavior, S/S mechanisms, leaching test and economic analysis, were investigated. The results show that the S/S efficiency of Mn(2+) and NH4(+)-N could reach 91.58% and 99.98%, respectively, and the pH value is 8.75 when the molar ratio of Mg:P is 3:1 and the dose of PM (MgO and Na3PO4·12H2O) is 8wt%...
October 18, 2017: Ecotoxicology and Environmental Safety
Narciso Couto, Liliya Davlyatova, Caroline A Evans, Phillip C Wright
RATIONALE: Post-translational modified peptides analysis by mass spectrometry (MS) remains incomplete, in part due to incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA). An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity. The use of broad band collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation...
October 21, 2017: Rapid Communications in Mass Spectrometry: RCM
Miglena Manandhar, John E Cronan
BioF (8-amino-7-oxononanoate synthase) is a strictly conserved enzyme that catalyzes the first step in assembly of the fused heterocyclic rings of biotin. The BioF acyl chain donor has long been thought to be pimeloyl-CoA. Indeed, in vitro the Escherichia coli and Bacillus sphaericus enzymes have been shown to condense pimeloyl-CoA with L-alanine in a pyridoxal 5' -phosphate-dependent reaction with concomitant CoA release and decarboxylation of L-alanine. However, recent in vivo studies of E. coli and Bacillus subtilis suggested that the BioF proteins of the two bacteria could have different specificities for pimelate thioesters in that E...
October 20, 2017: Applied and Environmental Microbiology
Scott R Clark, Keel Yong Lee, Hoyoung Lee, Jawahar Khetan, Hyun Chang Kim, Yun Hwa Choi, Kwanwoo Shin, You-Yeon Won
Polycations are used for a number of biological applications, including antibiotics and gene therapy. One aspect of the use of polycation gene carriers such as polyethylenemine (PEI) in gene therapy that is not well understood is their ability to escape from the vesicles they are internalized in. Here, in an attempt to gain a better understanding of PEI interaction with endosomal lipids under osmotic stress, we performed investigations using monolayers and vesicles derived from a mixture of neutral and negative lipids (1,2-dipalmitoylphosphatidylcholine (DPPC) and bis(monoacylglycero)phosphate (BMP), respectively)...
October 17, 2017: Acta Biomaterialia
Farahnaz Fahimipour, Erfan Dashtimoghadam, Morteza Rasoulianboroujeni, Mostafa Yazdimamaghani, Kimia Khoshroo, Mohammadreza Tahriri, Amir Yadegari, Jose A Gonzalez, Daryoosh Vashaee, Douglas C Lobner, Tahereh S Jafarzadeh Kashi, Lobat Tayebi
OBJECTIVE: A systematic characterization of hybrid scaffolds, fabricated based on combinatorial additive manufacturing technique and freeze-drying method, is presented as a new platform for osteoblastic differentiation of dental pulp cells (DPCs). METHODS: The scaffolds were consisted of a collagenous matrix embedded in a 3D-printed beta-tricalcium phosphate (β-TCP) as the mineral phase. The developed construct design was intended to achieve mechanical robustness owing to 3D-printed β-TCP scaffold, and biologically active 3D cell culture matrix pertaining to the Collagen extracellular matrix...
October 17, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
Carlos Ruiz Cánovas, Francisco Macías, Rafael Pérez López, José Miguel Nieto
This paper investigates the mobility and fluxes of REE, Y and Sc under weathering conditions from an anomalously metal-rich phosphogypsum stack in SW Spain. The interactions of the phosphogypsum stack with rainfall and organic matter-rich solutions, simulating the weathering processes observed due to its location on salt-marshes, were simulated by leaching tests (e.g. EN 12457-2 and TCLP). Despite the high concentration of REE, Y and Sc contained in the phosphogypsum stack, their mobility during the leaching tests was very low; <0...
October 17, 2017: Science of the Total Environment
Wen-Huai Wang, Yi Wang, Zhi Li, Cun-Zhi Wei, Jing-Chan Zhao, Lu-Qin Sun
A floating bed (FB) system vegetated with calamus, iris, lythrum, and Hydrocotyle vulgaris, and a strengthened FB (SFB) system with zeolite and sponge iron as fillers were simultaneously applied to purify urban landscape water in different zones. The urban landscape water, an artificial lake of approximately 326m(2), was supplied with reclaimed water during a six-month experiment. Results indicated that the concentrations of nitrogen (N) and phosphorus (P) in the SFB zone (SFBZ) were significantly lower than those in the control zone (CZ) and the FB zone (FBZ) after six months of operation...
October 17, 2017: Science of the Total Environment
Kai Lu, Ying Wang, Xia Chen, Zhichao Zhang, Yue Li, Wenru Li, Qiang Zhou
The widespread and extensive application of insecticides have promoted the development of resistance in the brown planthopper Nilaparvata lugens (Stål), one of the most important rice pests in Asia. To better understand the underlying molecular mechanisms of metabolic resistance to insecticides, a chlorpyrifos-resistant (CR) strain of N. lugens was selected and its possible resistance mechanism was investigated. Synergistic tests using carboxylesterases (CarEs) inhibitor triphenyl phosphate (TPP) decreased the resistance of N...
October 17, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
Yinan Wang, Choiwan Lau, Jianzhong Lu
Herein we exploited a novel target-initiated labeling strategy for the multiplex detection of microRNAs (miRNAs) by coupling duplex-specific nuclease (DSN) with terminal deoxynucleotidyl transferase (TdT). In the presence of target miRNA, the immobilized and 3'-blocked capture probes hybridized with target and thus the formed DNA-RNA hybrid was recognized by DSN. DSN mediated the digestion of 3'-phosphated capture probes (CPs) in the hybrids and synchronously target was released and recycled for another round of hybridization and cleavage...
November 1, 2017: Analytica Chimica Acta
Deeksha Munnur, Ivan Ahel
ADP-ribosylation is a chemical modification of macromolecules that plays an important role in regulation of quintessential biological processes such as DNA repair, transcription, chromatin remodelling, stress response, apoptosis, bacterial metabolism and many others. ADP-ribosylation is carried out by ADP-ribosyltransferase proteins, such as poly(ADP-ribose) polymerases (PARPs) that transfer either monomer or polymers of ADP-ribose onto the molecular targets by using nicotinamide adenine dinucleotide (NAD(+) ) as a cofactor...
October 20, 2017: FEBS Journal
Chia-Huang Chang, Ching-Jung Yu, Jung-Chieh Du, Hsien-Chih Chiou, Hsin-Chang Chen, Winnie Yang, Ming-Yi Chung, Ying-Sheue Chen, Betau Hwang, I-Fang Mao, Mei-Lien Chen
OBJECTIVE: The aim of this study was to clarify the association between organophosphate pesticides (OPs) and attention-deficit/hyperactivity disorder (ADHD) related to oxidative stress and genetic polymorphisms. METHODS: This case-control study enrolled 93 children with ADHD and 112 control children in north Taiwan. Six dialkyl phosphate (DAP) metabolites of OPs and oxidative stress biomarkers were analyzed. Polymorphisms of the dopamine receptor D4 gene (DRD4) were identified...
October 17, 2017: Environmental Research
Vikram J Pansare, Victoria Hwang, Carlos Figueroa, Robert K Prud'homme
Dense fluorescent pigments used for inkjet printing of UV and IR-readable non-photobleaching security features require stabilizers to prevent aggregation/sedimentation and inkjet head clogging at high resolution. A study of small molecule adsorption to α-alumina, a model system for security pigments, is presented. Alumina is dispersed by two methods yielding different zeta potentials but identical isoelectric points. Essentially complete dispersion is obtained in water at pH 3 but aggregation occurs at pH 6 where the surface charging is lower...
October 6, 2017: Journal of Colloid and Interface Science
Vera Carniello, Akshay K Harapanahalli, Henk J Busscher, Henny C van der Mei
Nisin-associated-sensitivity-response-regulator (NsaRS) in Staphylococcus aureus is important for its adhesion to surfaces and resistance against antibiotics, like nisin. NsaRS consists of an intra-membrane-located sensor NsaS and a cytoplasmatically-located response-regulator NsaR, which becomes activated upon receiving phosphate groups from the intra-membrane-located sensor. HYPOTHESIS: The intra-membrane location of the NsaS sensor leads us to hypothesize that the two-component NsaRS system not only senses "chemical" (nisin) but also "mechanical" (adhesion) stresses to modulate efflux of antibiotics from the cytoplasm...
October 7, 2017: Journal of Colloid and Interface Science
Inmaculada Couso, María Esther Pérez-Pérez, Enrique Martínez-Force, Hee-Sik Kim, Yonghua He, James G Umen, José L Crespo, Peter Bozhkov
Autophagy is an intracellular catabolic process that allows cells to recycle unneeded or damaged material to maintain cellular homeostasis. This highly dynamic process is characterized by the formation of double-membrane vesicles called autophagosomes, which engulf and deliver the cargo to the vacuole. Flow of material through the autophagy pathway and its degradation in the vacuole is known as autophagic flux, and reflects the autophagic degradation activity. A number of assays have been developed to determine autophagic flux in yeasts, mammals, and plants, but it has not been examined yet in algae...
October 19, 2017: Journal of Experimental Botany
Yin Sim Ng, Derek Juinn Chieh Chan
Macrophytes have been used to mitigate eutrophication and upgrade effluent quality via their nutrient removal capability. However, the available data are influenced by factors such as microbial activities, weather, and wastewater quality, making comparison between nutrient removal performance of different macrophytes almost impossible. In this study, phytoremediation by Spirodela polyrhiza, Salvinia molesta and Lemna sp. were carried out axenically in synthetic wastewater under controlled condition to precisely evaluate nutrient removal efficiency of NO3(-)-N, PO4(3-), NH3-N, COD and pH in the water sample...
October 20, 2017: International Journal of Phytoremediation
Daniel P Salem, Xun Gong, Albert Tianxiang Liu, Volodymyr B Koman, Juyao Dong, Michael S Strano
Single-stranded DNA (ssDNA) oligonucleotides have unique, and in some cases, sequence-specific molecular interactions with the surface of carbon nanotubes that remain the subject of fundamental study. In this work, we observe and analyze a generic, ionic strength mediated phase transition exhibited by over 25 distinct oligonucleotides adsorbed to single-walled carbon nanotubes (SWCNTs) in colloidal suspension. The phase transition occurs as monovalent salts are used to modify the ionic strength from 500 mM to 1 mM, causing a reversible reduction in the fluorescence quantum yield by as much as 90 percent...
October 20, 2017: Journal of the American Chemical Society
Mianqiang Xue, Liang Zhou, Naoya Kojima, Takashi Machimura, Akihiro Tokai
DecaBDE has been widely used as flame retardant in electrical and electronic equipment (EEE). It has recently been listed in Annex A of the Stockholm Convention. The time series flow, stock, and emission of DecaBDE in EEE in Japan were quantified. On this basis, a risk/risk trade-off analysis of substituting DecaBDE with triphenyl phosphate (TPhP) that is one possible phosphorus-based alternative was conducted. The stock of DecaBDE reached a maximum of ~42,000 t in 1995. Even though the demand flow was negligible in 2030, the stock was modelled to be still ~470 t...
October 20, 2017: Environmental Science & Technology
Ralf Greiner
Kinetic parameters for the dephosphorylation of sodium phytate and a series of partially phosphorylated myo-inositol phosphates were determined at pH 3.0 and pH 5.0 for three phytase preparations (Aspergillus niger, Escherichia coli, rye). The enzymes showed lower affinity and turnover numbers at pH 3 compared to pH 5 towards all myo-inositol phosphates included in the study. The number and distribution of phosphate groups on the myo-inositol ring affected the kinetic parameters. Representatives of the individual phytate dephosphorylation pathways were identified as the best substrates of the phytases...
October 20, 2017: Journal of Agricultural and Food Chemistry
Meadhbh A Brennan, Audrey Renaud, Fabien Guilloton, Miryam Mebarki, Valerie Trichet, Luc Sensebé, Frederic Deschaseaux, Nathalie Chevallier, Pierre Layrolle
The possibility of using adipose tissue-derived stromal cells (ATSC) as alternatives to bone marrow-derived stromal cells (BMSC) for bone repair has garnered interest due to the accessibility, high cell yield, and rapid in vitro expansion of ATSC. For clinical relevance, their bone forming potential in comparison to BMSC must be proven. Distinct differences between ATSC and BMSC have been observed in vitro and comparison of osteogenic potential in vivo is not clear to date. The aim of the current study was to compare the osteogenesis of human xenofree-expanded ATSC and BMSC in vitro and in an ectopic nude mouse model of bone formation...
October 19, 2017: Stem Cells Translational Medicine
Kang Chen, Dong Chen, Chuangxin Lan, Xiongfa Liang, Tao Zeng, Jian Huang, Xiaolu Duan, Zhenzhen Kong, Shujue Li, Hans-Göran Tiselius, Alberto Gurioli, Xiaogang Lu, Guohua Zeng, Wenqi Wu
OBJECTIVE: To investigate the impact of green tea on urinary oxalate excretion in healthy male volunteers. MATERIALS AND METHODS: The oxalate concentrations after different brewing times (2-60 min) of different qualities (2-8 g) of green tea were measured in in vitro experiment. In in vivo experiment, the effects on urine composition were assessed in 12 healthy men with an age of 24-29 years. Each subject was requested to collect two 24-h urine samples under normal dietary conditions...
October 19, 2017: International Urology and Nephrology
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