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https://www.readbyqxmd.com/read/29150735/glycomics-of-human-embryonic-stem-cells-and-human-induced-pluripotent-stem-cells
#1
REVIEW
Jun-Ichi Furukawa, Kazue Okada, Yasuro Shinohara
Most cells are coated by a dense glycocalyx composed of glycoconjugates such as glycosphingolipids, glycoproteins, and proteoglycans. The overall glycomic profile is believed to be crucial for the diverse roles of glycans, which are mediated by specific interactions that regulate cell-cell adhesion, the immune response, microbial pathogenesis, and other cellular events. Many cell surface markers were discovered and identified as glycoconjugates such as stage-specific embryonic antigen, Tra-1-60/81 and various other cell surface molecules (e...
November 18, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29150395/transradial-versus-transfemoral-arterial-access-in-liver-cancer-embolization-randomized-trial-to-assess-patient-satisfaction
#2
Ricardo Yamada, Stephen Bracewell, Beatriz Bassaco, Juan Camacho, M Bret Anderson, Andrew Conrad, Corie Lynn, Paul Burns, Heather Collins, Marcelo Guimaraes
PURPOSE: To determine whether transradial access (TRA) or transfemoral access (TFA) provides better patient satisfaction during intra-arterial therapy (IAT) for liver cancer. MATERIALS AND METHODS: This randomized, prospective, intra- and interpatient controlled trial compared TRA vs TFA accesses in patients with primary or metastatic liver cancer undergoing IAT. After having one of each type of access (1 TRA and 1 TFA), all patients selected their preferred access regardless of whether a third intervention was indicated...
November 15, 2017: Journal of Vascular and Interventional Radiology: JVIR
https://www.readbyqxmd.com/read/29141654/early-career-researchers-want-open-science
#3
Andrea Farnham, Christoph Kurz, Mehmet Ali Öztürk, Monica Solbiati, Oona Myllyntaus, Jordy Meekes, Tra My Pham, Clara Paz, Magda Langiewicz, Sophie Andrews, Liisa Kanninen, Chantal Agbemabiese, Arzu Tugce Guler, Jeffrey Durieux, Sarah Jasim, Olivia Viessmann, Stefano Frattini, Danagul Yembergenova, Carla Marin Benito, Marion Porte, Anaïs Grangeray-Vilmint, Rafael Prieto Curiel, Carin Rehncrona, Tareq Malas, Flavia Esposito, Kristina Hettne
Open Science is encouraged by the European Union and many other political and scientific institutions. However, scientific practice is proving slow to change. We propose, as early career researchers, that it is our task to change scientific research into open scientific research and commit to Open Science principles.
November 15, 2017: Genome Biology
https://www.readbyqxmd.com/read/29131513/role-of-vascular-ultrasound-scanning-in-repeated-trans-radial-coronary-artery-intervention-prospective-randomized-study
#4
Mahmoud Farouk Elmahdy, Mohamed Hassan, Ahmed Elguindy
OBJECTIVES: We aimed to detect if pre-procedure vascular ultrasound scanning (VUS) of radial arteries (RAs) can increase the radial access success (RAS) rate and/or reduce the vascular access time (VAT), by guiding the choice of the proper access site for repeated trans-radial interventions (TRIs). BACKGROUND: Currently, repeated-TRIs are encountered more frequently in most of the cath. labs. However, structural changes of the RAs after TRA may hinder it is usage for repeated-TRI...
November 13, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/29117652/microhardness-of-bulk-fill-composite-materials
#5
Katarina Kelić, Sanja Matić, Danijela Marović, Eva Klarić, Zrinka Tarle
The aim of the study was to determine microhardness of high- and low-viscosity bulk-fill composite resins and compare it with conventional composite materials. Four materials of high-viscosity were tested, including three bulk-fills: QuiXfi l (QF), x-tra fi l (XTF) and Tetric EvoCeram Bulk Fill (TEBCF), while nanohybrid composite GrandioSO (GSO) served as control. The other four were low-viscosity composites, three bulk-fill materials: Smart Dentin Replacement (SDR), Venus Bulk Fill (VBF) and x-tra base (XB), and conventional control material X-Flow (XF)...
December 2016: Acta Clinica Croatica
https://www.readbyqxmd.com/read/29115387/generation-of-human-induced-pluripotent-stem-cells-from-burn-patient-derived-skin-fibroblasts-using-a-non-integrative-method
#6
Shangfeng Fu, Jianwu Ding, Dewu Liu, Heping Huang, Min Li, Yang Liu, Longxiang Tu, Deming Liu
Patient specific induced pluripotent stem cells (iPSCs) have been recognized as a possible source of cells for skin tissue engineering. They have the potential to greatly benefit patients with large areas of burned skin or skin defects. However, the integration virus-based reprogramming method is associated with a high risk of genetic mutation and mouse embryonic fibroblast feeder-cells may be a pollutant. In the present study, human skin fibroblasts (HSFs) were successfully harvested from patients with burns and patient-specific iPSCs were generated using a non-integration method with a feeder-free approach...
October 23, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29106039/a-european-multicentre-retrospective-non-interventional-study-eu-treat-of-the-effectiveness-of-insulin-degludec-after-switching-basal-insulin-in-a-population-with-type-1-or-type-2-diabetes
#7
Thorsten Siegmund, Nikolaos Tentolouris, Søren T Knudsen, Annunziata Lapolla, Rudolf Prager, Tra-Mi Phan, Michael Lyng Wolden, Bernd Schultes
AIMS: To evaluate the clinical effectiveness of switching to insulin degludec (IDeg) in insulin-treated patients with either T1DM or T2DM, in conditions of routine clinical care. MATERIALS AND METHODS: This was a multicentre, retrospective, chart review study. All patients had their basal insulin switched to IDeg at least 6 months before the start of data collection. Baseline was defined as the most recent recording during the 3-month period before first prescription of IDeg...
November 6, 2017: Diabetes, Obesity & Metabolism
https://www.readbyqxmd.com/read/29105703/-disturbo-dissociativo-d-identit%C3%A3-auto-ipnosi-e-sintomi-simil-psicotici-da-un-caso-clinico-a-un-problema-di-diagnosi-differenziale
#8
Corinna Pancheri, Maria Caredda, Isabella Pacchiarotti, Roberto Delle Chiaie, Massimo Biondi
RIASSUNTO. Presentiamo il caso clinico di un paziente di 49 anni al suo esordio psicopatologico. La presenza di un'alterazione del tono dell'umore con sintomi schneideriani di primo rango nel contesto di un chiaro stato dissociativo simile alla trance ipnotica potrebbe portare, in un primo momento, a una diagnosi di disturbo bipolare avviando così il paziente verso un preciso iter terapeutico. Tuttavia riteniamo che la presenza di importanti segni di dissociazione associati alla pratica incontrollata dell'auto-ipnosi e alla presenza di "voci interne" in un paziente fortemente predisposto alla dissociazione permetta di porre una diagnosi sovraordinata di disturbo dissociativo di identità (DDI)...
September 2017: Rivista di Psichiatria
https://www.readbyqxmd.com/read/29105699/-fisiopatologia-dei-comportamenti-aggressivi-in-salute-mentale
#9
Enrico Pompili, Cristiano Carlone, Cristiana Silvestrini, Giuseppe Nicolò
RIASSUNTO. Introduzione. L'aggressività è un comportamento con origini evolutive, ma nella società di oggi è spesso distruttivo e disadattivo. Un aumento del comportamento aggressivo è presente in molte malattie mentali e rappresenta una sfida clinica per il servizio sanitario. Questi fenomeni possono portare ad agiti dannosi e violenti, rappresentando una grave preoccupazione per la salute pubblica. L'aggressività è spesso motivo di ospedalizzazione psichiatrica, e può portare a soggiorni ospedalieri prolungati, sofferenze dei pazienti e delle loro vittime con aumento della stigmatizzazione...
September 2017: Rivista di Psichiatria
https://www.readbyqxmd.com/read/29105400/effects-of-klf4-and-c-myc-knockdown-on-pluripotency-maintenance-in-porcine-induced-pluripotent-stem-cell
#10
Yu-Jing Liao, Yi-Shiou Chen, Ja-Xin Lee, Lih-Ren Chen, Jenn-Rong Yang
OBJECTIVES: The importance of Oct4 and Sox2 in maintaining pluripotency and self-renewal is well-understood, but the functions of Klf4 and c-Myc has not been fully investigated. In the present study, we attempted to determine the roles of Klf4 and c-Myc on pluripotency maintenance of porcine induced pluripotent stem (piPS) cells. MATERIALS AND METHODS: In this experimental study, we performed short hairpin RNA (shRNA) to knock down the Klf4 and c-Myc functions of piPS cells and examined pluripotency markers and teratoma formation to evaluate piPS cell pluripotency...
January 2018: Cell Journal
https://www.readbyqxmd.com/read/29102582/variation-in-the-adoption-of-transradial%C3%A2-access-for-st-segment-elevation-myocardial-infarction-insights-from-the-ncdr-cathpci-registry
#11
Javier A Valle, Lisa A Kaltenbach, Steven M Bradley, Robert W Yeh, Sunil V Rao, Hitinder S Gurm, Ehrin J Armstrong, John C Messenger, Stephen W Waldo
OBJECTIVES: The study sought to define patient, operator, and institutional factors associated with transradial access (TRA) in ST-segment elevation myocardial infarction (STEMI) percutaneous coronary intervention (PCI), the variation in use across operators and institutions, and the relationship with mortality and bleeding. BACKGROUND: TRA for PCI in STEMI is underutilized. Factors associated with TRA are not well described, nor is there variation across operators and institutions or their relationship with outcomes...
October 25, 2017: JACC. Cardiovascular Interventions
https://www.readbyqxmd.com/read/29100589/effect-of-access-site-choice-on-acute-kidney-injury-after-percutaneous-coronary-intervention
#12
Maitri S Pancholy, Kimberly Skelding, Thomas Scott, James Blankenship, Samir B Pancholy
Acute kidney injury (AKI) after percutaneous coronary intervention (PCI) is associated with worse outcomes. Consecutive patients undergoing PCI between 2005 and 2013 were retrospectively analyzed. Patients undergoing PCI using transfemoral access (TFA) were categorized as the TFA Group, and those using transradial access (TRA) were categorized as the TRA Group. Post-PCI AKI was defined as an increase in serum creatinine >0.5 mg/dl or >25% increase from baseline 48 to 72 hours after the procedure. Independent predictors of post-PCI AKI were identified using inverse probability weighted multivariable analysis...
September 20, 2017: American Journal of Cardiology
https://www.readbyqxmd.com/read/29090680/vim-1-carbapenemase-producing-escherichia-coli-isolated-from-retail-seafood-germany-2016
#13
Nicole Roschanski, Sebastian Guenther, Thi Thu Tra Vu, Jennie Fischer, Torsten Semmler, Stephan Huehn, Thomas Alter, Uwe Roesler
Carbapenems belong to the group of last resort antibiotics in human medicine. Therefore, the emergence of growing numbers of carbapenemase-producing bacteria in food-producing animals or the environment is worrying and an important concern for the public health sector. In the present study, a set of 45 Enterobacteriaceae isolated from German retail seafood (clams and shrimps), sampled in 2016, were investigated by real-time PCR for the presence of carbapenemase-producing bacteria. One Escherichia coli (ST10), isolated from a Venus clam (Ruditapes philippinarum) harvested in the Mediterranean Sea (Italy), contained the carbapenemase gene blaVIM-1 as part of the variable region of a class I integron...
October 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/29077101/exonuclease-assisted-target-recycling-amplification-for-label-free-chemiluminescence-assay-and-molecular-logic-operations
#14
Yongcun Yan, Shuzhen Yue, Tingting Zhao, Baoyu Luo, Sai Bi
A versatile exonuclease III (Exo III)-assisted target recycling amplification (Exo-TRA) strategy is demonstrated to achieve label-free and homogeneous chemiluminescence (CL) detection of target DNA. Inspired by this principle, a series of logic gates are constructed using DNA molecules as "inputs" to activate the Exo-TRA system.
October 27, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29061547/randomized-comparison-of-judkins-vs-tiger-catheter-in-coronary-angiography-via-the-right-radial-artery-the-judge-study
#15
Ioanna Xanthopoulou, Katerina Stavrou, Periklis Davlouros, Grigorios Tsigkas, Eleni Koufou, Georgios Almpanis, Michalis Koutouzis, Ioannis Tsiafoutis, Angelos Perperis, Athanasios Moulias, Nikolaos Koutsogiannis, George Hahalis
AIMS: To compare the performance of Tiger-II compared with Judkins 3.5L/4R catheters in coronary angiography (CAG) via the transradial approach (TRA). METHODS AND RESULTS: Consecutive patients undergoing non-urgent CAG via the right TRA were randomized to either Tiger-II (Terumo) or Judkins (3.5L/4R, Medtronic) 5Fr catheters and 320 patients in each group were randomized. Catheter or access site change was required in 57(17.8%) vs 68(21.3%) patients allocated in the Tiger-II and Judkins group respectively (p=0...
October 24, 2017: EuroIntervention
https://www.readbyqxmd.com/read/29059498/respiratory-reflex-generated-by-movement-of-the-arms
#16
Ranjit K Mal
In their article "Motor control of the trunk during a modified clean and jerk lift", Eriksson Crommert, Ekblom and Thortensson(1) measured the intra-abdominal pressure (IAP) and trunk muscle activity at various stages of lifting a 30-kg weight. They observed that the "TrA (transversus abdominis muscle) was more active in a position with augmented postural demand, i.e., with the arms above the head" and that "The activation of TrA was relatively low in the lower parts of the lift, despite a higher load in terms of imposed flexor moment (judged from the sketches in the figures, not calculated) corroborated by increases in ES activation and IAP amplitude, both providing the extensor moment necessary to raise the trunk...
October 23, 2017: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/29044931/a-novel-technique-for-simultaneous-hemostasis-of-ipsilateral-radial-and-ulnar-artery-access-sites
#17
Jonathan S Roberts, Jianli Niu
With the increased use of transradial artery access (TRA) for diagnostic and coronary interventional procedures, crossover to the ipsilateral ulnar artery after TRA failure is being reported more frequently. A major challenge with ipsilateral transradial and ulnar artery access is achieving efficient patent hemostasis of both the radial and ulnar arteries at the completion of the procedure. In this report, we describe two cases of failed TRA with subsequent ipsilateral ulnar artery access. A novel and practical technique of simultaneous patent hemostasis of both the ipsilateral radial and ulnar artery access sites is described, using a QuikClot(®) Radial(®) hemostasis pad and a TR Band(®) ...
October 17, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/29044358/-perch%C3%A3-la-legionellosi-%C3%A3-una-tra-le-pi%C3%A3-temibili-ica
#18
S Delia
La Legionellosi è una polmonite che può presentare un decorso variabile a seconda se i soggetti colpiti risiedono nel loro domicilio, sono ospiti di strutture recettive o sono ospedalizzati. In ambito ospedaliero, che annovera questa patologia tra le più pericolose Infezioni Correlate all'Assistenza (ICA), si registrano decisamente i casi più gravi e assai spesso letali. Gli errori nella Valutazione del Rischio e negli interventi di risanamento contribuiscono alla proliferazione all'interno delle condutture idriche di un temibile microrganismo che può essere eliminato o ridotto solo con un approccio multidisciplinare...
September 2017: La Clinica Terapeutica
https://www.readbyqxmd.com/read/29042079/cytotoxicity-and-dna-double-strand-breaks-in-human-gingival-fibroblasts-exposed-to-eluates-of-dental-composites
#19
Yang Yang, Franz-Xaver Reichl, Jianwei Shi, Xiuli He, Reinhard Hickel, Christof Högg
OBJECTIVE: Previously, single composite components were used to study cytotoxicity and induction of DNA double-strand breaks (DNA-DSBs) of dental composite resins. In the present study, cytotoxicity and induction of DNA-DSBs in human gingival fibroblasts (HGFs) were investigated with dental composite eluates consisting of multiple components. The eluates were qualified and quantified. METHODS: The composites Esthet.X(®) HD, Venus(®), X-tra fil(®), CLEARFIL™ AP-X, Admira(®) Fusion and QuiXfil(®) were polymerized and immersed into Dulbecco's modified Eagle's medium (DMEM) for 72h...
October 14, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29041962/comparative-assessment-of-an-gambiae-and-an-stephensi-mosquitoes-to-determine-transmission-reducing-activity-of-antibodies-against-p-falciparum-sexual-stage-antigens
#20
Maarten Eldering, Anaïs Bompard, Kazutoyo Miura, Will Stone, Isabelle Morlais, Anna Cohuet, Geert-Jan van Gemert, Patrick M Brock, Sanna R Rijpma, Marga van de Vegte-Bolmer, Wouter Graumans, Rianne Siebelink-Stoter, Dari F Da, Carole A Long, Merribeth J Morin, Robert W Sauerwein, Thomas S Churcher, Teun Bousema
BACKGROUND: With the increasing interest in vaccines to interrupt malaria transmission, there is a demand for harmonization of current methods to assess Plasmodium transmission in laboratory settings. Potential vaccine candidates are currently tested in the standard membrane feeding assay (SMFA) that commonly relies on Anopheles stephensi mosquitoes. Other mosquito species including Anopheles gambiae are the dominant malaria vectors for Plasmodium falciparum in sub-Saharan Africa. METHODS: Using human serum and monoclonal pre-fertilization (anti-Pfs48/45) and post-fertilization (anti-Pfs25) antibodies known to effectively inhibit sporogony, we directly compared SMFA based estimates of transmission-reducing activity (TRA) for An...
October 17, 2017: Parasites & Vectors
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