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https://www.readbyqxmd.com/read/28442158/diversity-and-evolutionary-aspects-of-mucin-binding-mucbp-domain-repeats-among-lactobacillus-plantarum-group-strains-through-comparative-genetic-analysis
#1
Sundru Manjulata Devi, Prakash M Halami
Mucin binding protein (Mub) of Lactobacillus plantarum- group (LPG) is considered as a potential marker gene due to its diversity and adherence property to host intestinal layer. We recently observed strain specific diversity in mub gene of LPG strains (n=8) and grouped them into Type I, II and III based on the insertion and/or deletion of MucBP domain repeats (Devi et al., 2016). The mub gene expression pattern noted by real-time PCR revealed 67.64 increased fold change in MTCC 9483 with four MucBP repeats...
April 12, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28440751/validation-in-daily-clinical-situations-of-diascope-%C3%A2-a-software-developed-to-help-healthcare-professionals-individualize-antidiabetic-treatment-in-type-2-diabetes
#2
Francisco Javier Ampudia-Blasco, Francisco Javier García-Soidán, Manuela Rubio Sánchez, Tra-Mi Phan
INTRODUCTION: DiaScope(®) is a software to help in individualized prescription of antidiabetic treatment in type 2 diabetes. This study assessed its value and acceptability by different professionals. MATERIAL AND METHODS: DiaScope(®) was developed based on the ADA-EASD 2012 algorithm and on the recommendation of 12 international diabetes experts using the RAND/UCLA appropriateness method. The current study was performed at a single session. In the first phase, 5 clinical scenarios were evaluated, selecting the most appropriated therapeutic option among 4 possibilities (initial test)...
March 2017: Endocrinol Diabetes Nutr
https://www.readbyqxmd.com/read/28437332/ultrasound-assessment-of-the-abdominal-muscles-at-rest-and-during-the-aslr-test-among-adolescents-with-scoliosis
#3
Paweł Linek, Edward Saulicz, Michał Kuszewski, Tomasz Wolny
STUDY DESIGN: Observational study. OBJECTIVE: Are there deviations in the thickness of abdominal muscles at rest in individuals with adolescent idiopathic scoliosis (AIS)? Are there deviations in abdominal muscular activity in people with AIS during the active straight leg raise (ASLR) test? SUMMARY OF BACKGROUND DATA: Ultrasound imaging can be used to assess the lateral abdominal muscle. Some authors suggest that scoliosis develops through a weakness or improper functioning of the abdominal muscles that cannot provide adequate support to the spinal segments...
May 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28430314/transulnar-approach-as-an-alternative-to-transradial-approach-in-non-coronary-intervention-safety-feasibility-and-technical-factors
#4
Adam Zybulewski, Martin Edwards, Edward Kim, Francis S Nowakowski, Rahul Patel, Nora Tabori, Robert Lookstein, Aaron Fischman
PURPOSE: Transulnar access (TUA) has been shown to be an effective alternative to transradial access (TRA) for coronary intervention. This study evaluates the safety and efficacy of TUA in patients undergoing visceral interventions in the setting of contraindication to TRA. MATERIALS AND METHODS: Patients who underwent visceral interventions via ulnar approach were included in the study. Outcome variables include technical success, access site and bleeding complications...
April 13, 2017: Journal of Vascular Access
https://www.readbyqxmd.com/read/28417059/susceptibility-of-human-oral-squamous-cell-carcinoma-oscc-h103-and-h376-cell-lines-to-retroviral-oskm-mediated-reprogramming
#5
Nalini Devi Verusingam, Swee Keong Yeap, Huynh Ky, Ian C Paterson, Suan Phaik Khoo, Soon Keng Cheong, Alan H K Ong, Tunku Kamarul
Although numbers of cancer cell lines have been shown to be successfully reprogrammed into induced pluripotent stem cells (iPSCs), reprogramming Oral Squamous Cell Carcinoma (OSCC) to pluripotency in relation to its cancer cell type and the expression pattern of pluripotent genes under later passage remain unexplored. In our study, we reprogrammed and characterised H103 and H376 oral squamous carcinoma cells using retroviral OSKM mediated method. Reprogrammed cells were characterized for their embryonic stem cells (ESCs) like morphology, pluripotent gene expression via quantitative real-time polymerase chain reaction (RT-qPCR), immunofluorescence staining, embryoid bodies (EB) formation and directed differentiation capacity...
2017: PeerJ
https://www.readbyqxmd.com/read/28410516/environmental-knowledge-and-attitudes-and-behaviours-towards-energy-consumption
#6
Arminda Paço, Tânia Lavrador
Numerous investigations have arisen in order to study and characterise environmentally friendly consumer profiles, with some authors applying the relationship between knowledge, attitudes and behaviour to this end. The present research approach, based upon the Theory of Reasoned Action (TRA), seeks to verify the existence of relationships between knowledge and attitudes and between knowledge and environmental behaviour. In this instance, data collection involved a questionnaire aimed at assessing the overall environmental knowledge of respondents as well as their attitudes and behaviours regarding energy issues (savings, consumption, interest, use)...
April 11, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28399443/the-effect-of-inspiratory-and-expiratory-loads-on-abdominal-muscle-activity-during-breathing-in-subjects-at-risk-for-the-development-of-chronic-obstructive-pulmonary-disease-and-healthy
#7
António Mesquita Montes, Carlos Crasto, Cristina Argel de Melo, Rita Santos, Susana Pereira, João Paulo Vilas-Boas
The abdominal muscle activity has been shown to be variable in subjects with chronic obstructive pulmonary disease (COPD) when respiratory demand increases and their recruitment pattern may change the mechanics, as well as the work and cost of breathing. The scientific evidence in subjects "at risk" for the development of COPD may be important to understand the natural history of this disease. This study aims to evaluate the effect of inspiratory and expiratory loads on the abdominal muscle activity during breathing in subjects "at risk" for the development of COPD and healthy...
March 31, 2017: Journal of Electromyography and Kinesiology
https://www.readbyqxmd.com/read/28396005/transradial-access-for-interventional-radiology-single-centre-procedural%C3%A2-and-clinical-outcome-analysis
#8
Avnesh S Thakor, Mohammed T Alshammari, David M Liu, John Chung, Stephen G F Ho, Gerald M Legiehn, Lindsay Machan, Aaron M Fischman, Rahul S Patel, Darren Klass
PURPOSE: The study sought to describe a single centre's technical approach to transradial intervention and report on clinical outcomes and safety. METHODS: A total of 749 transradial access (TRA) procedures were performed at a single hospital in 562 patients (174 women and 388 men). Procedures included 445 bland embolizations or chemoembolizations of the liver, 88 uterine artery embolizations, and 148 procedures for Selective Internal Radiation Therapy (Y90), which included mapping and administration...
April 7, 2017: Canadian Association of Radiologists Journal, Journal L'Association Canadienne des Radiologistes
https://www.readbyqxmd.com/read/28388892/self-administered-physical-exercise-training-as-treatment-of-neck-and-shoulder-pain-among-military-helicopter-pilots-and-crew-a-randomized-controlled-trial
#9
Mike Murray, Britt Lange, Bo Riebeling Nørnberg, Karen Søgaard, Gisela Sjøgaard
BACKGROUND: Neck pain is frequent among military helicopter pilots and crew-members, and pain may influence individual health and work performance. The aim of this study was to examine if an exercise intervention could reduce neck pain among helicopter pilots and crew-members. METHODS: Thirty-one pilots and thirty-eight crew-members were randomized to either an exercise-training-group (n = 35) or a reference-group (n = 34). The exercise-training-group received 20-weeks of specific neck/shoulder training...
April 7, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28382727/scalable-stirred-suspension-culture-for-the-generation-of-billions-of-human-induced-pluripotent-stem-cells-using-single-use-bioreactors
#10
Chee Keong Kwok, Yuichiro Ueda, Asifiqbal Kadari, Katharina Günther, Antoine Heron, Aletta C Schnitzler, Martha Rook, Frank Edenhofer
The production of human induced pluripotent stem cells (hiPSCs) in quantities that are relevant for cell-based therapies and cell-loaded implants through standard adherent culture is hardly achievable and lacks process scalability. A promising approach to overcoming these hurdles is the culture of hiPSCs in suspension. In this study, stirred suspension culture vessels were investigated for their suitability in the expansion of two hiPSC lines inoculated as a single cell suspension, with a free scalability between volumes of 50 and 2 400 mL...
April 6, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28363712/partial-cricotracheal-resection-for-severe-upper-tracheal-stenosis-potential-impacts-on-the-outcome
#11
Ahmed Musaad Abd El-Fattah, Hisham Atef Ebada, Hazem Emam Amer, Mohammed Mohammed Abosamra, Ali Tawfik
OBJECTIVE: The aim of this study was to investigate the potential impact of multiple preoperative and intraoperative variables on the outcome of partial cricotracheal resection and tracheal resection anastomosis (PCTR/TRA). METHODS: The study was conducted on 35 consecutive patients of grade III and IV upper tracheal stenosis with or without subglottic involvement. The indication of PCTR/TRA was post intubation stenosis in all patients. Overall complications (major and minor) occurred in 18 patients...
March 28, 2017: Auris, Nasus, Larynx
https://www.readbyqxmd.com/read/28359056/the-construction-and-identification-of-induced-pluripotent-stem-cells-derived-from-acute-myelogenous-leukemia-cells
#12
Liang-Fang Zhu, Qiu-Ru Chen, Shao-Zhen Chen, Ling-Yan Wang, Xiao-Feng Luo, Jin-Hua Ren, Xiao-Hong Yuan, Xue-Qiong Wu, Yan-Ling Zeng, Min Xiao, Yong-Quan Chen, Ying-Yu Chen, Min-Hui Lin, Zheng-Jun Wu, Zhi-Zhe Chen, Jian-Da Hu, Ting Yang
OBJECTIVE: The present study aimed to establish an induced pluripotent stem cell (iPSC) line from acute myelogenous leukemia (AML) cells in vitro and identify their biological characteristics. METHODS: Cells from the AML-infiltrated skin from an M6 patient were infected with a lentivirus carrying OCT4, SOX2, KLF4 and C-MYC to induce iPSCs. The characteristics of the iPSCs were confirmed by alkaline phosphatase (ALP) staining. The proliferation ability of iPSCs was detected with a CCK-8 assay...
March 29, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28358624/understanding-antiretroviral-treatment-adherence-among-hiv-positive-women-at-four-postpartum-time-intervals-qualitative-results-from-the-kabeho-study-in-rwanda
#13
Michelle M Gill, Aline Umutoni, Heather J Hoffman, Dieudonne Ndatimana, Gilles F Ndayisaba, Solange Kibitenga, Placidie Mugwaneza, Anita Asiimwe, Emily A Bobrow
As lifelong antiretroviral therapy (ART) for pregnant women is implemented, it is important to understand the attitudes and norms affecting women's postpartum ART adherence. This is a qualitative cross-sectional study of HIV-positive postpartum women (n = 112) enrolled in a 2-year observational prospective cohort in Rwanda. Informed by the Theory of Reasoned Action (TRA), we conducted in-depth interviews with women whose children were 0-6, 7-12, 13-18, or 21-24 months of age to describe factors contributing to adherence and changes over time...
March 30, 2017: AIDS Patient Care and STDs
https://www.readbyqxmd.com/read/28355416/accuracy-evaluation-of-three-modelling-tools-for-occupational-exposure-assessment
#14
Andrea Spinazzè, Filippo Lunghini, Davide Campagnolo, Sabrina Rovelli, Monica Locatelli, Andrea Cattaneo, Domenico M Cavallo
Objectives: The objective of this study is to evaluate the accuracy and robustness of three exposure-modelling tools [STOFFENMANAGER® v.6, European Centre for Ecotoxicology and Toxicology of Chemical Target Risk Assessment v.3.1 (ECETOC TRA v.3.1), and Advanced REACH Tool (ART v.1.5)], by comparing available measured data for exposure to organic solvents and pesticides in occupational exposure scenarios (ESs). Methods: Model accuracy was evaluated by comparing the predicted and the measured values, expressed as an underestimation or overestimation factor (PRED/EXP), and by regression analysis...
February 7, 2017: Annals of Work Exposures and Health
https://www.readbyqxmd.com/read/28352203/missing-data-and-multiple-imputation-in-clinical-epidemiological-research
#15
Alma B Pedersen, Ellen M Mikkelsen, Deirdre Cronin-Fenton, Nickolaj R Kristensen, Tra My Pham, Lars Pedersen, Irene Petersen
Missing data are ubiquitous in clinical epidemiological research. Individuals with missing data may differ from those with no missing data in terms of the outcome of interest and prognosis in general. Missing data are often categorized into the following three types: missing completely at random (MCAR), missing at random (MAR), and missing not at random (MNAR). In clinical epidemiological research, missing data are seldom MCAR. Missing data can constitute considerable challenges in the analyses and interpretation of results and can potentially weaken the validity of results and conclusions...
2017: Clinical Epidemiology
https://www.readbyqxmd.com/read/28332559/the-immunogenetics-of-narcolepsy-associated-with-a-h1n1-pdm09-vaccination-pandemrix-supports-a-potent-gene-environment-interaction
#16
I L Bomfim, F Lamb, K Fink, A Szakács, A Silveira, L Franzén, V Azhary, M Maeurer, N Feltelius, N Darin, T Hallböök, L Arnheim-Dahlström, I Kockum, T Olsson
The influenza A(H1N1)pdm09 vaccination campaign from 2009 to 2010 was associated with a sudden increase in the incidence of narcolepsy in several countries. Narcolepsy with cataplexy is strongly associated with the human leukocyte antigen (HLA) class II DQB1*06:02 allele, and protective associations with the DQB1*06:03 allele have been reported. Several non-HLA gene loci are also associated, such as common variants of the T-cell receptor-α (TRA), the purinergic receptor P2RY11, cathepsin H (CTSH) and TNFSF4/OX40L/CD252...
March 23, 2017: Genes and Immunity
https://www.readbyqxmd.com/read/28330035/pathologies-malformatives-du-tube-neural-d%C3%A3-pist%C3%A3-es-%C3%A3-l%C3%A2-%C3%A3-chographie-pr%C3%A3-natale-%C3%A3-abidjan
#17
Anhum Konan, Idrissa Garba, Alionou Sétchéou, Olivier Tra-Bi, Sorel Manéwa, Yves Broh, Abdoulaye Touré, Ali Coulibaly, N'goran Kouamé, Anne-Marie N'goan-Domoua, Roger D N'gbesso
No abstract text is available yet for this article.
March 2017: Journal of Neuroradiology. Journal de Neuroradiologie
https://www.readbyqxmd.com/read/28322521/-associazione-o-nesso-causale-tra-esposizioni-ed-esiti-bradford-hill-e-oltre
#18
Silvia Narduzzi, Roberta Pirastu, Ariana Zeka, Carla Ancona
No abstract text is available yet for this article.
January 2017: Epidemiologia e Prevenzione
https://www.readbyqxmd.com/read/28320889/trunk-muscle-activation-during-movement-with-a-new-exercise-device-for-lumbo-pelvic-reconditioning
#19
Tobias Weber, Dorothée Debuse, Sauro E Salomoni, Edith L Elgueta Cancino, Enrico De Martino, Nick Caplan, Volker Damann, Jonathan Scott, Paul W Hodges
Gravitational unloading leads to adaptations of the human body, including the spine and its adjacent structures, making it more vulnerable to injury and pain. The Functional Re-adaptive Exercise Device (FRED) has been developed to activate the deep spinal muscles, lumbar multifidus (LM) and transversus abdominis (TrA), that provide inter-segmental control and spinal protection. The FRED provides an unstable base of support and combines weight bearing in up-right posture with side alternating, elliptical leg movements, without any resistance to movement...
March 2017: Physiological Reports
https://www.readbyqxmd.com/read/28318944/cytotoxic-dammarane-type-triterpenoids-from-the-leaves-of-viburnum-sambucinum
#20
Thanh Tra Nguyen, Bich Ngan Truong, Huong Doan Thi Mai, Marc Litaudon, Van Hung Nguyen, Thao Do Thi, Van Minh Chau, Van Cuong Pham
Four new dammarane-type triterpenoids (1-4) and twelve known compounds (5-16) were isolated from the leaves of Viburnum sambucinum Reinw. ex Blume. Their structures were determined by spectral data analysis, including MS and 2D NMR. Cytotoxic activity evaluation in vitro against four cancer cell lines (KB, LU-1, HepG2 and MCF7) suggested that the octanor-dammarane derivatives were the main cytotoxic components of the leaves of V. sambucinum.
March 7, 2017: Bioorganic & Medicinal Chemistry Letters
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