Wu Su, Fanding Xu, Jinjin Zhong, Ranrui Hu, Lizhuo Wang, Hua Li, Zhiwei Yang, Shuai Ge, Huaizhen He, Shengli Han, Xiuying Xie, Hui Guo, Langchong He, Jiankang Liu, Tao Yi, Yu Kong, Jiangang Long
Carnitine palmitoyltransferase 1A (CPT1A), which resides on the mitochondrial outer membrane, serves as the rate-limiting enzyme of fatty acid β-oxidation. Identifying the compounds targeting CPT1A warrants a promising candidate for modulating lipid metabolism. In this study, we developed a CPT1A-overexpressed mitochondrial membrane chromatography (MMC) to screen the compounds with affinity for CPT1A. Cells overexpressing CPT1A were cultured, and subsequently, their mitochondrial membrane was isolated and immobilized on amino-silica gel cross-linked by glutaraldehyde...
February 27, 2024: ACS Applied Materials & Interfaces