Bingyin Peng, Sarah J Weintraub, Zeyu Lu, Samuel Evans, Qianyi Shen, Liam McDonnell, Manuel Plan, Thomas Collier, Li Chen Cheah, Lei Ji, Christopher B Howard, Will Anderson, Matt Trau, Geoff Dumsday, Erin L Bredeweg, Eric M Young, Robert Speight, Claudia E Vickers
The variability in phenotypic outcomes among biological replicates in engineered microbial factories presents a captivating mystery. Establishing the association between phenotypic variability and genetic drivers is important to solve this intricate puzzle. We applied a previously developed auxin-inducible depletion of hexokinase 2 as a metabolic engineering strategy for improved nerolidol production in Saccharomyces cerevisiae , and biological replicates exhibit a dichotomy in nerolidol production of either 3...
December 12, 2023: ACS Synthetic Biology