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https://www.readbyqxmd.com/read/27824887/the-bacteriohopanepolyol-inventory-of-novel-aerobic-methane-oxidising-bacteria-reveals-new-biomarker-signatures-of-aerobic-methanotrophy-in-marine-systems
#1
Darci Rush, Kate A Osborne, Daniel Birgel, Andreas Kappler, Hisako Hirayama, Jörn Peckmann, Simon W Poulton, Julia C Nickel, Kai Mangelsdorf, Marina Kalyuzhnaya, Frances R Sidgwick, Helen M Talbot
Aerobic methane oxidation (AMO) is one of the primary biologic pathways regulating the amount of methane (CH4) released into the environment. AMO acts as a sink of CH4, converting it into carbon dioxide before it reaches the atmosphere. It is of interest for (paleo)climate and carbon cycling studies to identify lipid biomarkers that can be used to trace AMO events, especially at times when the role of methane in the carbon cycle was more pronounced than today. AMO bacteria are known to synthesise bacteriohopanepolyol (BHP) lipids...
2016: PloS One
https://www.readbyqxmd.com/read/27760342/antiviral-cd8-t-cells-restricted-by-human-leukocyte-antigen-class-ii-exist-during-natural-hiv-infection-and-exhibit-clonal-expansion
#2
Srinika Ranasinghe, Pedro A Lamothe, Damien Z Soghoian, Samuel W Kazer, Michael B Cole, Alex K Shalek, Nir Yosef, R Brad Jones, Faith Donaghey, Chioma Nwonu, Priya Jani, Gina M Clayton, Frances Crawford, Janice White, Alana Montoya, Karen Power, Todd M Allen, Hendrik Streeck, Daniel E Kaufmann, Louis J Picker, John W Kappler, Bruce D Walker
CD8(+) T cell recognition of virus-infected cells is characteristically restricted by major histocompatibility complex (MHC) class I, although rare examples of MHC class II restriction have been reported in Cd4-deficient mice and a macaque SIV vaccine trial using a recombinant cytomegalovirus vector. Here, we demonstrate the presence of human leukocyte antigen (HLA) class II-restricted CD8(+) T cell responses with antiviral properties in a small subset of HIV-infected individuals. In these individuals, T cell receptor β (TCRβ) analysis revealed that class II-restricted CD8(+) T cells underwent clonal expansion and mediated killing of HIV-infected cells...
October 18, 2016: Immunity
https://www.readbyqxmd.com/read/27759389/disruption-of-de-novo-adenosine-triphosphate-atp-biosynthesis-abolishes-virulence-in-cryptococcus-neoformans
#3
Ross D Blundell, Simon J Williams, Samantha D M Arras, Jessica L Chitty, Kirsten L Blake, Daniel J Ericsson, Nidhi Tibrewal, Jurgen Rohr, Y Q Andre E Koh, Ulrike Kappler, Avril A B Robertson, Mark S Butler, Matthew A Cooper, Bostjan Kobe, James A Fraser
Opportunistic fungal pathogens such as Cryptococcus neoformans are a growing cause of morbidity and mortality among immunocompromised populations worldwide. To address the current paucity of antifungal therapeutic agents, further research into fungal-specific drug targets is required. Adenylosuccinate synthetase (AdSS) is a crucial enzyme in the adeosine triphosphate (ATP) biosynthetic pathway, catalyzing the formation of adenylosuccinate from inosine monophosphate and aspartate. We have investigated the potential of this enzyme as an antifungal drug target, finding that loss of function results in adenine auxotrophy in C...
September 9, 2016: ACS Infectious Diseases
https://www.readbyqxmd.com/read/27686459/esicm-lives-2016-part-three-milan-italy-1-5-october-2016
#4
T Velasquez, G Mackey, J Lusk, U G Kyle, T Fontenot, P Marshall, L S Shekerdemian, J A Coss-Bu, A Nishigaki, T Yatabe, T Tamura, K Yamashita, M Yokoyama, J C Ruiz-Rodriguez, B Encina, R Belmonte, I Troncoso, P Tormos, M Riveiro, J Baena, A Sanchez, J Bañeras, J Cordón, N Duran, A Ruiz, J Caballero, X Nuvials, J Riera, J Serra, A M F Rutten, S N M van Ieperen, E P H M Der Kinderen, T Van Logten, L Kovacikova, P Skrak, M Zahorec, U G Kyle, A Akcan-Arikan, J C Silva, G Mackey, J Lusk, M Goldsworthy, L S Shekerdemian, J A Coss-Bu, D Wood, D Harrison, R Parslow, P Davis, J Pappachan, S Goodwin, P Ramnarayan, S Chernyshuk, H Yemets, V Zhovnir, S M Pulitano', S De Rosa, A Mancino, G Villa, F Tosi, P Franchi, G Conti, B Patel, H Khine, A Shah, D Sung, L Singer, S Haghbin, S Inaloo, Z Serati, M Idei, T Nomura, N Yamamoto, Y Sakai, T Yoshida, Y Matsuda, Y Yamaguchi, S Takaki, O Yamaguchi, T Goto, N Longani, S Medar, I R Abdel-Aal, A S El Adawy, H M E H Mohammed, A N Mohamed, S M Parry, L D Knight, L Denehy, N De Morton, C E Baldwin, D Sani, G Kayambu, V Z M da Silva, P Phongpagdi, Z A Puthucheary, C L Granger, J E Rydingsward, C M Horkan, K B Christopher, D McWilliams, C Jones, E Reeves, G Atkins, C Snelson, L M Aitken, J Rattray, J Kenardy, A M Hull, A Ullman, R Le Brocque, M Mitchell, C Davis, B Macfarlane, J C Azevedo, L L Rocha, F F M De Freitas, A M Cavalheiro, N M Lucinio, M S Lobato, G Ebeling, A Kraegpoeth, E Laerkner, I De Brito-Ashurst, C White, S Gregory, L G Forni, E Flowers, A Curtis, C A Wood, K Siu, K Venkatesan, J B H Muhammad, L Ng, E Seet, N Baptista, A Escoval, E Tomas, R Agrawal, R Mathew, A Varma, E Dima, E Charitidou, E Perivolioti, M Pratikaki, C Vrettou, A Giannopoulos, S Zakynthinos, C Routsi, E Atchade, S Houzé, S Jean-Baptiste, G Thabut, C Genève, S Tanaka, B Lortat-Jacob, P Augustin, M Desmard, P Montravers, F J González de Molina, S Barbadillo, R Alejandro, F Álvarez-Lerma, J Vallés, R M Catalán, E Palencia, A Jareño, R M Granada, M L Ignacio, N Cui, D Liu, H Wang, L Su, H Qiu, R Li, K Jaffal, A Rouzé, J Poissy, B Sendid, S Nseir, E Paramythiotou, M Rizos, F Frantzeskaki, A Antoniadou, S Vourli, L Zerva, A Armaganidis, J Riera, J Gottlieb, M Greer, O Wiesner, M Martínez, M Acuña, J Rello, T Welte, E Atchade, T Mignot, S Houzé, S Jean-Baptiste, G Thabut, B Lortat-Jacob, S Tanaka, P Augustin, M Desmard, P Montravers, S Soussi, E Dudoignon, A Ferry, M Chaussard, M Benyamina, A Alanio, S Touratier, M Chaouat, M Lafaurie, M Mimoun, A Mebazaa, M Legrand, M A Sheils, C Patel, L Mohankumar, N Akhtar, S K Pacheco Noriega, N Navarrete Aldana, J L Ávila León, J Durand Baquero, F Fernández Bernal, E Ahmadnia, J S Hadley, M Millar, D Hall, H Hewitt, H Yasuda, M Sanui, T Komuro, S Kawano, K Andoh, H Yamamoto, E Noda, J Hatakeyama, N Saitou, H Okamoto, A Kobayashi, T Takei, S Matsukubo, H B Rotzel, A Serrano Lázaro, D Aguillón Prada, M Rodriguez Gimillo, O Diaz Barinas, M L Blasco Cortes, J Ferreres Franco, J M Segura Roca, A Carratalá, B Gonçalves, R Turon, A Mendes, F Miranda, P J Mata, D Cavalcanti, N Melo, P Lacerda, P Kurtz, C Righy, L E de la Cruz Rosario, S P Gómez Lesmes, J C García Romero, A N García Herrera, E D Díaz Pertuz, M J Gómez Sánchez, E Regidor Sanz, J Barado Hualde, A Ansotegui Hernández, J M Guergué Irazabal, V Spatenkova, O Bradac, P Suchomel, T Urli, E Heusch Lazzeri, R Aspide, M Zanello, L Perez-Borrero, J M Garcia-Alvarez, M D Arias-Verdu, E Aguilar-Alonso, R Rivera-Fernandez, J Mora-Ordoñez, C De La Fuente-Martos, E Castillo-Lorente, F Guerrero-Lopez, S P Gómez Lesmes, L E De la Cruz Rosario, E D Díaz Pertuz, A Ansotegui Hernández, J C García Romero, M J Gómez Sánchez, A N García Herrera, J Roldán Ramírez, E Regidor Sanz, J Barado Hualde, J P Tirapu León, L Navarro-Guillamón, S Cordovilla-Guardia, A Iglesias-Santiago, F Guerrero-López, E Fernández-Mondéjar, A Vidal, M Perez, A Juez, N Arias, L Colino, J L Perez, H Pérez, P Calpe, M A Alcala, D Robaglia, C Perez, S K Lan, M M Cunha, T Moreira, F Santos, E Lafuente, M J Fernandes, J G Silva, L E de la Cruz Rosario, S P Gómez Lesmes, A N García Herrera, J C García Romero, E D Díaz Pertuz, M J Gómez Sánchez, E Regidor Sanz, J G Armando Echeverría, A Ansotegui Hernández, J Barado Hualde, V Podlepich, E Sokolova, E Alexandrova, K Lapteva, P Kurtz, C Shuinotsuka, L Rabello, G Vianna, A Reis, C Cairus, J Salluh, F Bozza, J C Barrios Torres, N J Fernández Araujo, P García-Olivares, E Keough, M Dalorzo, L K Tang, I De Sousa, M Díaz, L J Marcos-Zambrano, J E Guerrero, S E Zamora Gomez, G D Hernandez Lopez, A I Vazquez Cuellar, O R Perez Nieto, J A Castanon Gonzalez, D Bhasin, S Rai, H Singh, O Gupta, M K Bhattal, S Sampley, K Sekhri, R Nandha, F A Aliaga, F Olivares, F Appiani, P Farias, F Alberto, A Hernández, S Pons, R Sonneville, L Bouadma, M Neuville, E Mariotte, A Radjou, J Lebut, S Chemam, G Voiriot, M P Dilly, B Mourvillier, R Dorent, P Nataf, M Wolff, J F Timsit, O Ediboglu, S Ataman, H Ozkarakas, C Kirakli, A Vakalos, V Avramidis, O Obukhova, I A Kurmukov, S Kashiya, E Golovnya, V N Baikova, T Ageeva, T Haritydi, E V Kulaga, J J Rios-Toro, L Perez-Borrero, E Aguilar-Alonso, M D Arias-Verdu, J M Garcia-Alvarez, C Lopez-Caler, C De La Fuente-Martos, S Rodriguez-Fernandez, M Gomez Sanchez-Orézzoli, F Martin-Gallardo, J Nikhilesh, V Joshi, E Villarreal, J Ruiz, M Gordon, A Quinza, J Gimenez, M Piñol, A Castellanos, P Ramirez, Y D Jeon, W Y Jeong, M H Kim, I Y Jeong, M Y Ahn, J Y Ahn, S H Han, J Y Choi, Y G Song, J M Kim, N S Ku, H Shah, F Kellner, F Rezai, N Mistry, P Yodice, V Ovnanian, K Fless, E Handler, R Martínez Alejos, J D Martí Romeu, D González Antón, A Quinart, A Torres Martí, M Llaurado-Serra, A Lobo-Civico, A Ventura-Rosado, A Piñol-Tena, M Pi-Guerrero, C Paños-Espinosa, M Peralvo-Bernat, J Marine-Vidal, R Gonzalez-Engroba, N Montesinos-Cerro, M Treso-Geira, A Valeiras-Valero, L Martinez-Reyes, A Sandiumenge, M F Jimenez-Herrera, S Helyar, P Riozzi, A Noon, G Hallows, H Cotton, J Keep, P A Hopkins, A Taggu, S Renuka, S Sampath, P J T Rood, T Frenzel, R Verhage, M Bonn, P Pickkers, J G van der Hoeven, M van den Boogaard, F Corradi, L Melnyk, F Moggia, R Pienovi, G Adriano, C Brusasco, L Mariotti, M Lattuada, M J Bloomer, M Coombs, K Ranse, R Endacott, B Maertens, K Blot, S Blot, M P van Nieuw Amerongen, E S van der Heiden, J W R Twisk, A R J Girbes, J J Spijkstra, P Riozzi, S Helyar, H Cotton, G Hallows, A Noon, C Bell, K Peters, A Feehan, J Keep, P A Hopkins, K Churchill, K Hawkins, R Brook, N Paver, R Endacott, N Maistry, A van Wijk, N Rouw, T van Galen, S Evelein-Brugman, A Taggu, B Krishna, S Sampath, A Putzu, M Fang, M Boscolo Berto, A Belletti, T Cassina, L Cabrini, M Mistry, Y Alhamdi, I Welters, S T Abrams, C H Toh, H S Han, E M Gil, D S Lee, C M Park, S Winder-Rhodes, R Lotay, J Doyle, M W Ke, W C Huang, C H Chiang, W T Hung, C C Cheng, K C Lin, S C Lin, K R Chiou, S R Wann, C W Shu, P L Kang, G Y Mar, C P Liu, S Dubó, A Aquevedo, M Jibaja, D Berrutti, C Labra, R Lagos, M F García, V Ramirez, M Tobar, F Picoita, C Peláez, D Carpio, L Alegría, C Hidalgo, K Godoy, J Bakker, G Hernández, Y Sadamoto, K Katabami, T Wada, Y Ono, K Maekawa, M Hayakawa, A Sawamura, S Gando, H Marin-Mateos, J L Perez-Vela, R Garcia-Gigorro, M A Corres Peiretti, M J Lopez-Gude, S Chacon-Alves, E Renes-Carreño, J C Montejo-González, K L Parlevliet, H R W Touw, M Beerepoot, C Boer, P W G Elbers, P R Tuinman, S A Abdelmonem, T A Helmy, I El Sayed, S Ghazal, S H Akhlagh, M Masjedi, K Hozhabri, E Kamali, I Zýková, B Paldusová, P Sedlák, D Morman, A M Youn, Y Ohta, M Sakuma, D Bates, T Morimoto, P L Su, W Y Chang, W C Lin, C W Chen, F Facchin, F Zarantonello, G Panciera, A De Cassai, A Venrdramin, A Ballin, T Tonetti, P Persona, C Ori, L Del Sorbo, S Rossi, G Vergani, M Cressoni, D Chiumello, C Chiurazzi, M Brioni, I Algieri, T Tonetti, M Guanziroli, A Colombo, I Tomic, A Colombo, F Crimella, E Carlesso, V Gasparovic, L Gattinoni, A Serpa Neto, M Schmidt, T Pham, A Combes, M Gama de Abreu, P Pelosi, M J Schultz, B H Katira, D Engelberts, R E Giesinger, C Ackerley, T Yoshida, D Zabini, G Otulakowski, M Post, W M Kuebler, P J McNamara, B P Kavanagh, R Pirracchio, M Resche Rigon, M Carone, S Chevret, D Annane, S Eladawy, M El-Hamamsy, N Bazan, M Elgendy, G De Pascale, M S Vallecoccia, S L Cutuli, V Di Gravio, M A Pennisi, G Conti, M Antonelli, D T Andreis, W Khaliq, M Singer, J Hartmann, S Harm, S Alcantara Carmona, P Matia Almudevar, A Naharro Abellán, J Veganzones Ramos, L Pérez Pérez, B Lobo Valbuena, N Martínez Sanz, I Fernández Simón, M Arrigo, E Feliot, N Deye, A Cariou, B Guidet, S Jaber, M Leone, M Resche-Rigon, A Vieillard Baron, M Legrand, E Gayat, A Mebazaa, M Balik, I Kolnikova, M Maly, P Waldauf, G Tavazzi, J Kristof, A Herpain, F Su, E Post, F Taccone, J L Vincent, J Creteur, C Lee, F Hatib, Z Jian, S Buddi, M Cannesson, S Fileković, M Turel, R Knafelj, V Gorjup, R Stanić, P Gradišek, O Cerović, T Mirković, M Noč, J Tirkkonen, H Hellevuo, K T Olkkola, S Hoppu, K C Lin, W T Hung, C C Chiang, W C Huang, W C Juan, S C Lin, C C Cheng, P H Lin, K Y Fong, D S Hou, P L Kang, S R Wann, Y S Chen, G Y Mar, C P Liu, M Paul, W Bougouin, G Geri, F Dumas, B Champigneulle, S Legriel, J Charpentier, J P Mira, C Sandroni, A Cariou, J Zimmerman, E Sullivan, M Noursadeghi, B Fox, D Sampson, L McHugh, T Yager, S Cermelli, T Seldon, S Bhide, R A Brandon, R B Brandon, J Zwaag, R Beunders, P Pickkers, M Kox, F Gul, M K Arslantas, D Genc, N Zibandah, L Topcu, T Akkoc, I Cinel, E Greco, M P Lauretta, D T Andreis, M Singer, I Palacios Garcia, M Cordero, A Diaz Martin, T Aldabó Pallás, J Garnacho Montero, J Revuelto Rey, L Roman Malo, A A Tanaka Montoya, A D C Amador Martinez, L Y Delgado Ayala, E Monares Zepeda, J Franco Granillo, J Aguirre Sanchez, G Camarena Alejo, A Rugerio Cabrera, A Pedraza Montenegro, T Pham, G Beduneau, F Schortgen, L Piquilloud, E Zogheib, M Jonas, F Grelon, I Runge, N Terzi, S Grangé, G Barberet, P G Guitard, J P Frat, A Constan, J M Chrétien, J Mancebo, A Mercat, J C M Richard, L Brochard, E Soilemezi, E Koco, S Savvidou, C Nouris, D Matamis, R Di Mussi, S Spadaro, C A Volta, M Mariani, A Colaprico, C Antonio, F Bruno, S Grasso, A Rodriguez, I Martín-Loeches, E Díaz, J R Masclans, F Gordo, J Solé-Violán, M Bodí, F X Avilés-Jurado, S Trefler, M Magret, L F Reyes, J Marín-Corral, J C Yebenes, A Esteban, A Anzueto, S Aliberti, M I Restrepo, J Skytte Larsson, B Redfors, S E Ricksten, R Haines, J Powell-Tuck, H Leonard, M Ostermann, R E Berthelsen, T S Itenov, A Perner, J U Jensen, M Ibsen, A E K Jensen, M H Bestle, T Bucknall, J Dixon, F Boa, I MacPhee, B J Philips, J Doyle, F Saadat, T Samuels, S Huddart, B McCormick, R DeBrunnar, J Preece, M Swart, C Peden, S Richardson, L Forni, P Kalfon, K Baumstarck, P Estagnasie, M A Geantot, A Berric, G Simon, B Floccard, T Signouret, M Boucekine, M Fromentin, M Nyunga, A Sossou, M Venot, R Robert, A Follin, A Renault, M Garrouste, O Collange, Q Levrat, I Villard, D Thévenin, J Pottecher, R G Patrigeon, N Revel, C Vigne, O Mimoz, P Auquier, S Pawar, T Jacques, K Deshpande, R Pusapati, B Wood, R A Pulham, J Wray, K Brown, C Pierce, S Nadel, P Ramnarayan, J R Azevedo, W S Montenegro, D P Rodrigues, S C Sousa, V F Araujo, A L Leitao, P H Prazeres, A V Mendonca, M P Paula, A Das Neves, C I Loudet, M Busico, D Vazquez, D Villalba, A Lischinsky, M Veronesi, M Emmerich, E Descotte, A Juliarena, M Carboni Bisso, M Grando, A Tapia, M Camargo, D Villani Ulla, L Corzo, H Placido Dos Santos, A Ramos, J A Doglia, E Estenssoro, M Carbonara, S Magnoni, C L Mac Donald, J S Shimony, V Conte, F Triulzi, F Stretti, M Macrì, A Z Snyder, N Stocchetti, D L Brody, V Podlepich, V Shimanskiy, I Savin, K Lapteva, A Chumaev, M C Tjepkema-Cloostermans, J Hofmeijer, A Beishuizen, H Hom, M J Blans, M J A M van Putten, L Longhi, B Frigeni, M Curinga, D Mingone, S Beretta, A Patruno, L Gandini, A Vargiolu, F Ferri, R Ceriani, M R Rottoli, L Lorini, G Citerio, S Pifferi, M Battistini, V Cordolcini, A Agarossi, R Di Rosso, F Ortolano, N Stocchetti, C Mora Lourido, J L Santana Cabrera, J D Martín Santana, L Melián Alzola, C García Del Rosario, H Rodríguez Pérez, R Lorenzo Torrent, S Eslami, A Dalhuisen, T Fiks, M J Schultz, A Abu Hanna, P E Spronk, M Wood, D Maslove, J Muscedere, S H Scott, T Saha, A Hamilton, D Petsikas, D Payne, J G Boyd, Z A Puthucheary, A S McNelly, J Rawal, B Connolly, M J McPhail, P Sidhu, A Rowlerson, J Moxham, S D Harridge, N Hart, H E Montgomery, T Jovaisa, B Thomas, D Gupta, D S Wijayatilake, H P Shum, H S King, K C Chan, K B Tang, W W Yan, C Castro Arias, J Latorre, A Suárez De La Rica, E Maseda Garrido, A Montero Feijoo, C Hernández Gancedo, A López Tofiño, F Gilsanz Rodríguez, L K Gemmell, R Campbell, P Doherty, A MacKay, N Singh, S Vitaller, H Nagib, J Prieto, A Del Arco, B Zayas, C Gomez, S Tirumala, S A Pasha, B K Kumari, P Martinez-Lopez, A Puerto-Morlán, P Nuevo-Ortega, L Martinez Pujol, R Algarte Dolset, B Sánchez González, S Quintana Riera, J Trenado Álvarez, S Quintana, L Martínez, R Algarte, B Sánchez, J Trenado, E Tomas, N Brock, E Viegas, E Filipe, D Cottle, T Traynor, M V Trasmonte Martínez, M Pérez Márquez, L Colino Gómez, N Arias Martínez, J M Milicua Muñoz, B Quesada Bellver, M Muñoz Varea, M Á Alcalá Llorente, C Pérez Calvo, S D Hillier, M C Faulds, H Hendra, N Lawrence, K Maekawa, M Hayakawa, Y Ono, A Kodate, Y Sadamoto, N Tominaga, A Mizugaki, H Murakami, T Yoshida, K Katabami, T Wada, A Sawamura, S Gando, S Silva, L Kerhuel, B Malagurski, G Citerio, R Chabanne, S Laureys, L Puybasset, L Nobile, E R Pognuz, A O Rossetti, F Verginella, N Gaspard, J Creteur, N Ben-Hamouda, M Oddo, F S Taccone, Y Ono, M Hayakawa, H Iijima, K Maekawa, A Kodate, Y Sadamoto, A Mizugaki, H Murakami, K Katabami, T Wada, A Sawamura, S Gando, A Kodate, K Katabami, T Wada, Y Ono, K Maekawa, M Hayakawa, A Sawamura, S Gando, L W Andersen, T Raymond, R Berg, V Nadkarni, A Grossestreuer, T Kurth, M Donnino, A Krüger, P Ostadal, M Janotka, D Vondrakova, N Kongpolprom, J Cholkraisuwat, P T Pekkarinen, G Ristagno, S Masson, R Latini, S Bendel, T Ala-Kokko, T Varpula, J Vaahersalo, S Hoppu, M Tiainen, M M Mion, M Plebani, V Pettilä, M B Skrifvars, Y Son, K S Kim, G J Suh, W Y Kwon, J I Ko, M J Park, F Zama Cavicchi, E Iesu, L Nobile, J L Vincent, J Creteur, F S Taccone, H Tanaka, N Otani, S Ode, S Ishimatsu, L Martínez, R Algarte, B Sánchez, I Romero, F Martínez, S Quintana, J Trenado, D Vondrakova, P Ostadal, A Kruger, M Janotka, F Malek, P Neuzil, Y C Yeh, Y S Chen, C H Wang, C H Huang, A Chao, C T Lee, C H Lai, W S Chan, Y J Cheng, W Z Sun, S Kaese, C Horstmann, P Lebiedz, M Mourad, P Gaudard, J Eliet, N Zeroual, P Colson, P Ostadal, M Mlcek, M Hrachovina, A Kruger, D Vondrakova, M Janotka, M Mates, P Hala, O Kittnar, P Neuzil, A Jacky, A Rudiger, D R Spahn, D A Bettex, A Kara, S Akin, D Dos Reis Miranda, A Struijs, K Caliskan, R J van Thiel, E A Dubois, W de Wilde, F Zijlstra, D Gommers, C Ince, L Marca, A Xini, W Mongkolpun, C P R Cordeiro, R T Leite, O Lheureux, A Bader, L Rincon, C Santacruz, J C Preiser, A Chao, A S Chao, Y S Chen, W Kim, C Ahn, Y Cho, T H Lim, J Oh, K S Choi, B H Jang, J K Ha, A Mecklenburg, J Stamm, G Soeffker, M Kubik, K Sydow, H Reichenspurner, S Kluge, S Braune, B Bergantino, F Ruberto, E Magnanimi, E Privato, V Zullino, K Bruno, F Pugliese, G Sales, V Girotto, F Vittone, L Brazzi, C Fritz, A Kimmoun, F Vanhuyse, B Trifan, S Orlowski, E Albuisson, N Tran, B Levy, V Chhor, J Joachim, A Follin, B Champigneulle, J Chatelon, G Fave, J Mantz, R Pirracchio, D Díaz Diaz, M Villanova, M Aguirregabyria, G Andrade, L López, E Palencia, G John, R Cowan, R Hart, K Lake, K Litchfield, J W Song, Y J Lee, Y J Cho, S Choi, P Vermeir, D Vandijck, S Blot, A Mariman, R Verhaeghe, M Deveugele, D Vogelaers, L Chok, E B Bachli, D Bettex, S R Cottini, E Keller, M Maggiorini, R Schuepbach, T Fiks, C Stiphout, M Grevelink, I Vaneker, A Ruijter, M Buise, P E Spronk, S Altaba Tena, L Galarza Barrachina, J H Rodriguez Portillo, G Pagés Aznar, L Mateu Campos, M D Ferrándiz Sellés, M Arlandis Tomás, A Belenguer Muncharaz, L Skinner, S Monsalvo, E Olavarria, R Stümpfle, S J Na, J Park, C R Chung, C M Park, G Y Suh, J H Yang, T Witter, C Brousseau, M B Butler, M Erdogan, P C Mac Dougall, R S Green, T E F Abbott, H D T Torrance, N Cron, N Vaid, J Emmanuel, S S Siddiqui, N Prabu, H K Chaudhari, V P Patil, J V Divatia, S Solanki, A P Kulkarni, L A Rincon Gutierrez, A Bader, A Brasseur, O Lheureux, J L Vincent, J Creteur, F S Taccone, D Hempel, N Stauffert, F Recker, T Schröder, S Reusch, J Schleifer, R Breitkreutz, F Sjövall, A Perner, M Hylander Møller, R B Moraes, F K Borges, J A V Guillen, W J C Zabaletta, J Ruiz-Ramos, P Ramirez, M R Marqués-Miñana, E Villarreal, M Gordon, M Sosa, P Concha, A Castellanos, R Menendez, C Sánchez Ramírez, M Cabrera Santana, L Caipe Balcázar, S Hípola Escalada, M A Hernández Viera, C F Lübbe Vázquez, J J Díaz Díaz, F Artiles 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Pont, A Kotsopoulos, N Jansen, W F Abdo, A Gopcevic, Z Gavranovic, M Vucic, M Zlatic Glogoski, L Videc Penavic, A Horvat, L Martin-Villen, J J Egea-Guerero, J Revuelto-Rey, T Aldabo-Pallas, E Correa-Chamorro, A I Gallego-Corpa, P Ruiz Del Portal-Ruiz Granados, V Faivre, L Wildenberg, B Huot, A C Lukaszewicz, M Simsir, C Mengelle, D Payen, N Martinez Sanz, B Lobo Valbuena, M Valdivia de la Fuente, P Matía Almudena, L Pérez Pérez, S Alcántara Carmona, A Navarro Abellán, I Fernández Simón, J J Rubio Muñoz, J Veganzones Ramos, S Alcantara Carmona, P Matia Almudevar, A Naharro Abellan, M A Perez Lucendo, L Perez Perez, J Palamidessi Dominguez, R Fernandez Rivas, P Rodriguez Villamizar, S Wee, C Ong, Y H Lau, Y Wong, M E Banderas-Bravo, V Olea-Jiménez, J M Mora-Ordóñez, C Gómez-Jiménez, J L Muñoz-Muñoz, J Vallejo-Báez, D Daga-Ruiz, M Lebrón-Gallardo, G Rialp, J M Raurich, I Morán, M C Martín, G Heras, A Mas, I Vallverdú, S Hraiech, J Bourenne, C Guervilly, J M Forel, M Adda, P Sylla, A Mouaci, M Gainnier, L Papazian, P R Bauer, A Kumbamu, M E Wilson, J K Pannu, J S Egginton, R Kashyap, O Gajic, S Yoshihiro, M Sakuraya, M Hayakawa, A Hirata, N Kawamura, T Tsutui, K Yoshida, Y Hashimoto, C H Chang, H C Hu, L C Chiu, C Y Hung, S H Li, K C Kao, S Sibley, J Drover, C D'Arsigny, C Parker, D Howes, S Moffatt, J Erb, R Ilan, D Messenger, I Ball, J G Boyd, M Harrison, S Ridi, J Muscedere, A H Andrade, R C Costa, V A Souza, V Gonzalez, V Amorim, F Rolla, C A C Abreu Filho, R Miranda, S Atchasiri, P Buranavanich, T Wathanawatthu, S Suwanpasu, C Bureau, C Rolland-Debord, T Poitou, M Clavel, S Perbet, N Terzi, A Kouatchet, T Similowski, A Demoule, P Diaz, J Nunes, S Escórcio, G Silva, S Chaves, M Jardim, M Câmara, N Fernandes, R Duarte, J J Jardim, C A Pereira, J J Nóbrega, C M Chen, C C Lai, K C Cheng, W Chou, S J Lee, Y S Cha, W Y Lee, M Onodera, E Nakataki, J Oto, H Imanaka, M Nishimura, A Khadjibaev, D Sabirov, A Rosstalnaya, R Akalaev, F Parpibaev, E Antonucci, P Rossini, S Gandolfi, E Montini, S Orlando, M van Nes, F Karachi, S Hanekom, A H Andrade, U V Pereira, C A C Abreu Filho, R C Costa, M S W Parkin, M Moore, A H Andrade, R C Costa, K V Silva Carvalho, C A C Abreu Filho, H J Min, H J Kim, D S Lee, Y Y Choi, E Y Lee, I Song, D J Kim, Y Y E, J W Kim, J S Park, Y J Cho, J H Lee, J W Suh, Y H Jo, K S Kim, Y J Lee, J Ferrero-Calleja, D Merino-Vega, A I González-Jiménez, M Sigcha Sigcha, A Hernández-Tejedor, A Martin-Vivas, Á Gabán-Díez, R Ruiz-de Luna, N De la Calle-Pedrosa, I Temprano-Gómez, D Afonso-Rivero, J I Pellin-Ariño, A Algora-Weber, R R L Fumis, A B Ferraz, J M Vieira Junior, H Kirca, O Cakin, M Unal, H Mutlu, A Ramazanoglu, M Cengiz, E A Nicolini, F G F Pelisson, R S Nunes, S L da Silva, M M Carreira, F Bellissimo-Rodrigues, M A Ferez, A Basile-Filho, H C Chao, C M Chen, L Chen, M Hravnak, G Clermont, M Pinsky, A Dubrawski, J Luján Varas, R Molina Montero, L Alcázar Sánchez-Elvira, P Villa Díaz, C Pintado Delgado, B Llorente Ruiz, A Pardo Guerrero, J A Cambronero Galache, H Sherif, H Hassanin, R El Hossainy, W Samy, H Ly, H David, P Burtin, C Charpentier, M Barral, P Courant, E Fournel, L Gaide-Chevronnay, M Durand, P Albaladejo, J F Payen, O Chavanon, A Blandino Ortiz, S Pozzebon, O Lheureux, A Brasseur, J L Vincent, J Creteur, F S Taccone, F Fumagalli, S Scala, R Affatato, M De Maglie, D Zani, D Novelli, C Marra, A Luciani, D De Zani, M Luini, T Letizia, D Pravettoni, L Staszewsky, S Masson, A Belloli, M Di Giancamillo, E Scanziani, R Latini, G Ristagno, Y C Kye, G J Suh, W Y Kwon, K S Kim, K M Yu, G Babini, G Ristagno, L Grassi, F Fumagalli, S Bendel, M De Maglie, R Affatato, S Masson, R Latini, E Scanziani, M Reinikainen, M Skrifvars, F Kappler, M Blobner, S J Schaller, A Roasio, E Costanzo, S Cardellino, E Iesu, F Zama Cavicchi, V Fontana, L Nobile, J L Vincent, J Creteur, F S Taccone, M Park, K M You, G J Suh, W Y Kwon, S B Ko, K S Kim, A Xini, L Marca, O Lheureux, A Brasseur, J L Vincent, J Creteur, F S Taccone, A Beane, M C K T Thilakasiri, A P De Silva, T Stephens, C S Sigera, P Athapattu, S Jayasinghe, A Padeniya, R Haniffa, A Iglesias Santiago, V Chica Sáez, R de la Chica Ruiz-Ruano, A Sánchez González, N Kunze-Szikszay, S Wand, P Klapsing, A Wetz, T Heyne, K Schwerdtfeger, M Troeltzsch, M Bauer, M Quintel, O Moerer, D J Cook, W B Rutherford, D C Scales, N K Adhikari, B H Cuthbertson, T Suzuki, T Takei, K Fushimi, M Iwamoto, S Nakagawa, N Mendsaikhan, T Begzjav, G Lundeg, M W Dünser, D González Romero, J L Santana Cabrera, J D Martín Santana, Y Santana Padilla, H Rodríguez Pérez, R Lorenzo Torrent, R Kleinpell, I Chouris, V Radu, M Stougianni, A Lavrentieva, D Lagonidis, R D T Price, A Day, N Arora, M A Henderson, S Hickey, M I Almeida Costa, J P Carvalho, A A Gomes, P J Mergulhão, K K C Chan, H P Shum, W W Yan, B Maghsoudi, S H Tabei, M Masjedi, G Sabetian, H R Tabatabaei, A Akbarzadeh, S Saigal, A Pakhare, R Joshi, S K Pattnaik, B Ray, A F Rousseau, L Michel, M Bawin, E Cavalier, J Y 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Regidor Sanz, M J Gómez Sánchez, S Aldunate Calvo, A N García Herrera, J Barado Hualde, O Agudo Pascual, J P Tirapu León, A Corona, C Ruffini, A Spazzadeschi, F Marrazzo, A Gandola, R Sciurti, C Savi, E Catena, M W Ke, C C Cheng, W C Huang, C H Chiang, W T Hung, K C Lin, S C Lin, S R Wann, K R Chiou, C J Tseng, P L Kang, G Y Mar, C P Liu, P Bertini, F De Sanctis, F Guarracino, P Bertini, R Baldassarri, F Guarracino, S H Buitinck, P H J van der Voort, J Oto, E Nakataki, Y Tsunano, M Izawa, N Tane, M Onodera, M Nishimura, S Ghosh, A Gupta, A De Gasperi, E Mazza, R Limuti, M Prosperi, N Bissenova, A Yergaliyeva, L Talan, G Yılmaz, G Güven, F Yoruk, N D Altıntas, D N Mukherjee, L K Agarwal, K Mandal, M Palomar, B Balsera, M Vallverdu, M Martinez, M Garcia, D Castellana, R Lopez, F Barcenilla, G E Kaminsky, R Carreño, A Escribá, M Fuentes, V Gálvez, R Del Olmo, B Nieto, C Vaquerizo, J Alvarez, M A De la Torre, E Torres, E Bogossian, S Aranha Nouer, D Ribeiro Salgado, S Carvalho Brugger, G 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No abstract text is available yet for this article.
September 2016: Intensive Care Medicine Experimental
https://www.readbyqxmd.com/read/27588497/%C3%AE-secretase-inhibitor-i-inhibits-neuroblastoma-cells-with-notch-and-the-proteasome-among-its-targets
#5
Carmen Dorneburg, Annika V Goß, Matthias Fischer, Frederik Roels, Thomas F E Barth, Frank Berthold, Roland Kappler, Franz Oswald, Jens T Siveke, Jan J Molenaar, Klaus-Michael Debatin, Christian Beltinger
As high-risk neuroblastoma (NB) has a poor prognosis, new therapeutic modalities are needed. We therefore investigated the susceptibility of NB cells to γ-secretase inhibitor I (GSI-I). NOTCH signaling activity, the cellular effects of GSI-I and its mechanisms of cytotoxicity were evaluated in NB cells in vitro and in vivo. The results show that NOTCH signaling is relevant for human NB cells. Of the GSIs screened in vitro GSI-I was the most effective inhibitor of NB cells. Both MYCN-amplified and non-amplified NB cells were susceptible to GSI-I...
August 30, 2016: Oncotarget
https://www.readbyqxmd.com/read/27492680/size-dependent-microbial-oxidation-and-reduction-of-magnetite-nano-and-micro-particles
#6
James M Byrne, Gerrit van der Laan, Adriana I Figueroa, Odeta Qafoku, Chongmin Wang, Carolyn I Pearce, Michael Jackson, Joshua Feinberg, Kevin M Rosso, Andreas Kappler
The ability for magnetite to act as a recyclable electron donor and acceptor for Fe-metabolizing bacteria has recently been shown. However, it remains poorly understood whether microbe-mineral interfacial electron transfer processes are limited by the redox capacity of the magnetite surface or that of whole particles. Here we examine this issue for the phototrophic Fe(II)-oxidizing bacteria Rhodopseudomonas palustris TIE-1 and the Fe(III)-reducing bacteria Geobacter sulfurreducens, comparing magnetite nanoparticles (d ≈ 12 nm) against microparticles (d ≈ 100-200 nm)...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27376885/formamides-as-lewis-base-catalysts-in-sn-reactions-efficient-transformation-of-alcohols-into-chlorides-amines-and-ethers
#7
Peter H Huy, Sebastian Motsch, Sarah M Kappler
A simple formamide catalyst facilitates the efficient transformation of alcohols into alkyl chlorides with benzoyl chloride as the sole reagent. These nucleophilic substitutions proceed through iminium-activated alcohols as intermediates. The novel method, which can be even performed under solvent-free conditions, is distinguished by an excellent functional group tolerance, scalability (>100 g) and waste-balance (E-factor down to 2). Chiral substrates are converted with excellent levels of stereochemical inversion (99 %→≥95 % ee)...
August 16, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27307436/identification-of-shared-tcr-sequences-from-t-cells-in-human-breast-cancer-using-emulsion-rt-pcr
#8
Daniel J Munson, Colt A Egelston, Kami E Chiotti, Zuly E Parra, Tullia C Bruno, Brandon L Moore, Taizo A Nakano, Diana L Simons, Grecia Jimenez, John H Yim, Dmitri V Rozanov, Michael T Falta, Andrew P Fontenot, Paul R Reynolds, Sonia M Leach, Virginia F Borges, John W Kappler, Paul T Spellman, Peter P Lee, Jill E Slansky
Infiltration of T cells in breast tumors correlates with improved survival of patients with breast cancer, despite relatively few mutations in these tumors. To determine if T-cell specificity can be harnessed to augment immunotherapies of breast cancer, we sought to identify the alpha-beta paired T-cell receptors (TCRs) of tumor-infiltrating lymphocytes shared between multiple patients. Because TCRs function as heterodimeric proteins, we used an emulsion-based RT-PCR assay to link and amplify TCR pairs. Using this assay on engineered T-cell hybridomas, we observed ∼85% accurate pairing fidelity, although TCR recovery frequency varied...
July 19, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27234371/anaerobic-microbial-fe-ii-oxidation-and-fe-iii-reduction-in-coastal-marine-sediments-controlled-by-organic-carbon-content
#9
Katja Laufer, James M Byrne, Clemens Glombitza, Caroline Schmidt, Bo Barker Jørgensen, Andreas Kappler
Coastal marine sediments contain varying concentrations of iron, oxygen, nitrate and organic carbon. It is unknown how organic carbon content influences the activity of nitrate-reducing and phototrophic Fe(II)-oxidizers and microbial Fe-redox cycling in such sediments. Therefore, microcosms were prepared with two coastal marine sediments (Kalø Vig and Norsminde Fjord at Aarhus Bay, Denmark) varying in TOC from 0.4 to 3.0 wt%. The microcosms were incubated under light/dark conditions with/without addition of nitrate and/or Fe(II)...
September 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27195003/polychromatic-iterative-statistical-material-image-reconstruction-for-photon-counting-computed-tomography
#10
Thomas Weidinger, Thorsten M Buzug, Thomas Flohr, Steffen Kappler, Karl Stierstorfer
This work proposes a dedicated statistical algorithm to perform a direct reconstruction of material-decomposed images from data acquired with photon-counting detectors (PCDs) in computed tomography. It is based on local approximations (surrogates) of the negative logarithmic Poisson probability function. Exploiting the convexity of this function allows for parallel updates of all image pixels. Parallel updates can compensate for the rather slow convergence that is intrinsic to statistical algorithms. We investigate the accuracy of the algorithm for ideal photon-counting detectors...
2016: International Journal of Biomedical Imaging
https://www.readbyqxmd.com/read/27140143/versatile-variable-temperature-insert-at-the-deimos-beamline-for-in-situ-electrical-transport-measurements
#11
L Joly, B Muller, E Sternitzky, J G Faullumel, A Boulard, E Otero, F Choueikani, J P Kappler, M Studniarek, M Bowen, P Ohresser
The design and the first experiments are described of a versatile cryogenic insert used for its electrical transport capabilities. The insert is designed for the cryomagnet installed on the DEIMOS beamline at the SOLEIL synchrotron dedicated to magnetic characterizations through X-ray absorption spectroscopy (XAS) measurements. This development was spurred by the multifunctional properties of novel materials such as multiferroics, in which, for example, the magnetic and electrical orders are intertwined and may be probed using XAS...
May 2016: Journal of Synchrotron Radiation
https://www.readbyqxmd.com/read/26916055/a-comparison-of-dual-energy-x-ray-absorptiometry-air-displacement-plethysmography-and-a-mode-ultrasound-to%C3%A2-assess-body-composition-in-college-age-adults
#12
Kelly E Johnson, Brian Miller, Ann L Gibson, Trisha A McLain, Judith A Juvancic-Heltzel, Rachele M Kappler, Ronald Otterstetter
BACKGROUND: There is currently a dearth of scientific literature exploring the agreement of A-mode ultrasound (US) body composition analysis with dual-energy X-ray absorptiometry (DXA) and air displacement plethysmography (ADP). OBJECTIVE: To investigate the agreement of US with DXA and ADP for estimating fat mass (FM) and fat-free mass (FFM). METHODS: College-age men (n = 33) and women (n = 41) volunteered to participate in this study...
February 24, 2016: Clinical Physiology and Functional Imaging
https://www.readbyqxmd.com/read/26869619/accuracy-of-genomic-prediction-in-switchgrass-panicum-virgatum-l-improved-by-accounting-for-linkage-disequilibrium
#13
Guillaume P Ramstein, Joseph Evans, Shawn M Kaeppler, Robert B Mitchell, Kenneth P Vogel, C Robin Buell, Michael D Casler
Switchgrass is a relatively high-yielding and environmentally sustainable biomass crop, but further genetic gains in biomass yield must be achieved to make it an economically viable bioenergy feedstock. Genomic selection (GS) is an attractive technology to generate rapid genetic gains in switchgrass, and meet the goals of a substantial displacement of petroleum use with biofuels in the near future. In this study, we empirically assessed prediction procedures for genomic selection in two different populations, consisting of 137 and 110 half-sib families of switchgrass, tested in two locations in the United States for three agronomic traits: dry matter yield, plant height, and heading date...
2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/26835839/evaluation-of-conventional-imaging-performance-in-a-research-whole-body-ct-system-with-a-photon-counting-detector-array
#14
Zhicong Yu, Shuai Leng, Steven M Jorgensen, Zhoubo Li, Ralf Gutjahr, Baiyu Chen, Ahmed F Halaweish, Steffen Kappler, Lifeng Yu, Erik L Ritman, Cynthia H McCollough
This study evaluated the conventional imaging performance of a research whole-body photon-counting CT system and investigated its feasibility for imaging using clinically realistic levels of x-ray photon flux. This research system was built on the platform of a 2nd generation dual-source CT system: one source coupled to an energy integrating detector (EID) and the other coupled to a photon-counting detector (PCD). Phantom studies were conducted to measure CT number accuracy and uniformity for water, CT number energy dependency for high-Z materials, spatial resolution, noise, and contrast-to-noise ratio...
February 21, 2016: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/26818529/human-imaging-with-photon-counting-based-computed-tomography-at-clinical-dose-levels-contrast-to-noise-ratio-and-cadaver-studies
#15
Ralf Gutjahr, Ahmed F Halaweish, Zhicong Yu, Shuai Leng, Lifeng Yu, Zhoubo Li, Steven M Jorgensen, Erik L Ritman, Steffen Kappler, Cynthia H McCollough
OBJECTIVES: The purpose of this work was to measure and compare the iodine contrast-to-noise ratio (CNR) between a commercial energy-integrating detector (EID) computed tomography (CT) system and a photon-counting detector (PCD) CT scanner capable of human imaging at clinical dose rates, as well as to determine clinical feasibility using human cadavers. MATERIALS AND METHODS: A research dual-source PCD-CT scanner was used, where the "A" tube/detector subsystem used an EID and the "B" tube/detector subsystem used a PCD...
July 2016: Investigative Radiology
https://www.readbyqxmd.com/read/26687009/structural-basis-of-interprotein-electron-transfer-in-bacterial-sulfite-oxidation
#16
Aaron P McGrath, Elise L Laming, G Patricia Casas Garcia, Marc Kvansakul, J Mitchell Guss, Jill Trewhella, Benoit Calmes, Paul V Bernhardt, Graeme R Hanson, Ulrike Kappler, Megan J Maher
Interprotein electron transfer underpins the essential processes of life and relies on the formation of specific, yet transient protein-protein interactions. In biological systems, the detoxification of sulfite is catalyzed by the sulfite-oxidizing enzymes (SOEs), which interact with an electron acceptor for catalytic turnover. Here, we report the structural and functional analyses of the SOE SorT from Sinorhizobium meliloti and its cognate electron acceptor SorU. Kinetic and thermodynamic analyses of the SorT/SorU interaction show the complex is dynamic in solution, and that the proteins interact with Kd = 13...
December 19, 2015: ELife
https://www.readbyqxmd.com/read/26594204/maturation-of-molybdoenzymes-and-its-influence-on-the-pathogenesis-of-non-typeable-haemophilus-influenzae
#17
Rabeb Dhouib, Dk S M Pg Othman, Ama-Tawiah Essilfie, Phil M Hansbro, Jeffrey O Hanson, Alastair G McEwan, Ulrike Kappler
Mononuclear molybdenum enzymes of the dimethylsulfoxide (DMSO) reductase family occur exclusively in prokaryotes, and a loss of some these enzymes has been linked to a loss of bacterial virulence in several cases. The MobA protein catalyzes the final step in the synthesis of the molybdenum guanine dinucleotide (MGD) cofactor that is exclusive to enzymes of the DMSO reductase family. MobA has been proposed as a potential target for control of virulence since its inhibition would affect the activities of all molybdoenzymes dependent upon MGD...
2015: Frontiers in Microbiology
https://www.readbyqxmd.com/read/26490540/seven-site-versus-three-site-method-of-body-composition-using-bodymetrix-ultrasound-compared-to-dual-energy-x-ray-absorptiometry
#18
Marissa N Baranauskas, Kelly E Johnson, Judith A Juvancic-Heltzel, Rachele M Kappler, Laura Richardson, Scott Jamieson, Ronald Otterstetter
: Obesity is a steadily growing epidemic affecting all segments of the population including college-aged students. The weight gain that is evidenced amid the transitional stage of college years increases the risks associated with cardiovascular and metabolic diseases. The BodyMetrix® BX-2000 (ULTRA) using a seven-site method has been evaluated against dual-energy X-ray absorptiometry (DXA) for estimation of body composition, which has yielded conflicting results. To date, no studies have compared the three-site method Jackson and Pollock three-site method to DXA...
October 22, 2015: Clinical Physiology and Functional Imaging
https://www.readbyqxmd.com/read/26248795/fatty-acid-binding-proteins-expressed-at-the-human-blood-brain-barrier-bind-drugs-in-an-isoform-specific-manner
#19
Gordon S Lee, Katharina Kappler, Christopher J H Porter, Martin J Scanlon, Joseph A Nicolazzo
PURPOSE: To examine the expression of fatty acid binding proteins (FABPs) at the human blood-brain barrier (BBB) and to assess their ability to bind lipophilic drugs. METHODS: mRNA and protein expression of FABP subtypes in immortalized human brain endothelial (hCMEC/D3) cells were examined by RT-qPCR and Western blot, respectively. FABPs that were found in hCMEC/D3 cells (hFABPs) were recombinantly expressed and purified from Escherichia coli C41(DE3) cells. Drug binding to these hFABPs was assessed using a fluorescence assay, which measured the ability of a panel of lipophilic drugs to displace the fluorescent probe compound 1-anilinonaphthalene-8-sulfonic acid (ANS)...
October 2015: Pharmaceutical Research
https://www.readbyqxmd.com/read/26129650/metal-specific-cd4-t-cell-responses-induced-by-beryllium-exposure-in-hla-dp2-transgenic-mice
#20
M T Falta, A N Tinega, D G Mack, N A Bowerman, F Crawford, J W Kappler, C Pinilla, A P Fontenot
Chronic beryllium disease (CBD) is a granulomatous lung disorder that is associated with the accumulation of beryllium (Be)-specific CD4(+) T cells into the lung. Genetic susceptibility is linked to HLA-DPB1 alleles that possess a glutamic acid at position 69 (βGlu69), and HLA-DPB1*02:01 is the most prevalent βGlu69-containing allele. Using HLA-DP2 transgenic (Tg) mice, we developed a model of CBD that replicates the major features of the human disease. Here we characterized the T-cell receptor (TCR) repertoire of Be-responsive CD4(+) T cells derived from the lungs of Be oxide-exposed HLA-DP2 Tg mice...
January 2016: Mucosal Immunology
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