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https://www.readbyqxmd.com/read/27922179/a-carbon-dioxide-stripping-model-for-mammalian-cell-culture-in-manufacturing-scale-bioreactors
#1
Zizhuo Xing, Amanda M Lewis, Michael C Borys, Zheng Jian Li
Control of carbon dioxide within the optimum range is important in mammalian bioprocesses at the manufacturing scale in order to ensure robust cell growth, high protein yields, and consistent quality attributes. The majority of bioprocess development work is done in laboratory bioreactors, in which carbon dioxide levels are more easily controlled. Some challenges in carbon dioxide control can present themselves when cell culture processes are scaled up, because carbon dioxide accumulation is a common feature due to longer gas-residence time of mammalian cell culture in large scale bioreactors...
December 6, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27918355/experienced-dsaek-surgeon-s-transition-to-dmek-outcomes-comparing-the-last-100-dsaek-surgeries-with-the-first-100-dmek-surgeries-exclusively-using-previously-published-techniques
#2
Paul M Phillips, Louis J Phillips, Valliamai Muthappan, Charlene M Maloney, Christopher N Carver
PURPOSE: To compare outcomes of Descemet stripping automated endothelial keratoplasty (DSAEK) and Descemet membrane endothelial keratoplasty (DMEK) when an experienced DSAEK surgeon transitions to DMEK while following 2 published endothelial keratoplasty surgical techniques. METHODS: This is a retrospective review of 200 eyes of 132 patients with Fuchs corneal dystrophy that underwent endothelial keratoplasty performed by one surgeon. Published, standardized techniques were followed for both DSAEK and DMEK...
December 1, 2016: Cornea
https://www.readbyqxmd.com/read/27917930/strongly-confined-spoof-surface-plasmon-polaritons-waveguiding-enabled-by-planar-staggered-plasmonic-waveguides
#3
Longfang Ye, Yifan Xiao, Yanhui Liu, Liang Zhang, Guoxiong Cai, Qing Huo Liu
We demonstrate a novel route to achieving highly efficient and strongly confined spoof surface plasmon polaritons (SPPs) waveguides at subwavelength scale enabled by planar staggered plasmonic waveguides (PSPWs). The structure of these new waveguides consists of an ultrathin metallic strip with periodic subwavelength staggered double groove arrays supported by a flexible dielectric substrate, leading to unique staggered EM coupling and waveguiding phenomenon. The spoof SPP propagation properties, including dispersion relations and near field distributions, are numerically investigated...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27916536/a-new-lateral-flow-strip-assay-lfsa-using-a-pair-of-aptamers-for-the-detection-of-vaspin
#4
Nurul Hanun Ahmad Raston, Van-Thuan Nguyen, Man Bock Gu
A new lateral flow strip assay (LFSA) using a pair of aptamers has been designed and successfully developed using a pair of aptamers-functionalized with gold nanoparticles (AuNPs). This new LFSA biosensor system utilizes a cognate aptamer duo binding to vaspin, a target protein, at the two different binding sites, and exhibited a sensitive and highly selective response to vaspin. A sandwich-type format in LFSA was developed based on biotin-labeled primary V1 aptamer immobilized on streptavidin coated membrane as a capturing probe and secondary V49 aptamer conjugated with AuNPs as a signaling probe...
November 28, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27916235/the-rohingya-people-of-myanmar-health-human-rights-and-identity
#5
REVIEW
Syed S Mahmood, Emily Wroe, Arlan Fuller, Jennifer Leaning
The Rohingya people of Myanmar (known as Burma before 1989) were stripped of citizenship in 1982, because they could not meet the requirement of proving their forefathers settled in Burma before 1823, and now account for one in seven of the global population of stateless people. Of the total 1·5 million Rohingya people living in Myanmar and across southeast Asia, only 82 000 have any legal protection obtained through UN-designated refugee status. Since 2012, more than 159 000 people, most of whom are Rohingya, have fled Myanmar in poorly constructed boats for journeys lasting several weeks to neighbouring nations, causing hundreds of deaths...
December 1, 2016: Lancet
https://www.readbyqxmd.com/read/27914563/differential-effects-of-testosterone-and-estradiol-on-clitoral-function-an-experimental-study-in-rats
#6
Paolo Comeglio, Ilaria Cellai, Sandra Filippi, Chiara Corno, Francesca Corcetto, Annamaria Morelli, Elena Maneschi, Elisa Maseroli, Edoardo Mannucci, Massimiliano Fambrini, Mario Maggi, Linda Vignozzi
INTRODUCTION: Female sexual response is a complex phenomenon in which psychological, neurologic, and vascular mechanisms and hormonal factors interact. During the arousal phase, they cooperate to increase genital blood flow, thus inducing engorgement of the clitoris and lubrication of the vagina. Regulation of vascular and non-vascular smooth muscle tone is the crucial event in the erectile process. Preclinical studies have suggested that nitric oxide (NO) is the main vasodilator neurotransmitter modulating, through the second messenger cyclic guanosine monophosphate (cGMP), clitoral flow vessels...
December 2016: Journal of Sexual Medicine
https://www.readbyqxmd.com/read/27911378/iridium-oxide-reduced-graphene-oxide-nanohybrid-thin-film-modified-screen-printed-electrodes-as-disposable-electrochemical-paper-microfluidic-ph-sensors
#7
Jiang Yang, Tae-Joon Kwak, Xiaodong Zhang, Robert McClain, Woo-Jin Chang, Sundaram Gunasekaran
A facile, controllable, inexpensive and green electrochemical synthesis of IrO2-graphene nanohybrid thin films is developed to fabricate an easy-to-use integrated paper microfluidic electrochemical pH sensor for resource-limited settings. Taking advantages from both pH meters and strips, the pH sensing platform is composed of hydrophobic barrier-patterned paper micropad (µPAD) using polydimethylsiloxane (PDMS), screen-printed electrode (SPE) modified with IrO2-graphene films and molded acrylonitrile butadiene styrene (ABS) plastic holder...
November 22, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27910415/a-multi-cone-x-ray-imaging-bragg-crystal-spectrometer
#8
M Bitter, K W Hill, Lan Gao, P C Efthimion, L Delgado-Apariccio, S Lazerson, N Pablant
This article describes a new x-ray imaging Bragg crystal spectrometer, which-in combination with a streak camera or a gated strip detector-can be used for time-resolved measurements of x-ray line spectra at the National Ignition Facility and other high power laser facilities. The main advantage of this instrument is that it produces perfect images of a point source for each wavelength in a selectable spectral range and that the detector plane can be perpendicular to the crystal surface or inclined by an arbitrary angle with respect to the crystal surface...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27909967/selective-blockade-of-%C3%AE-2-adrenoceptor-subtypes-modulates-contractility-of-rat-myocardium
#9
T L Zefirov, L I Khisamieva, N I Ziyatdinova, A L Zefirov
The study examined the dose-dependent effects of selective antagonists of α2A/D-, α2B-, and α2C- adrenoceptors applied in concentrations of 10(-9)-10(-5) M on atrial and ventricular contractility of rat myocardium in vitro. Selective blockade of each α2-adrenoceptor subtype affected the contractile force of the atrial and ventricular strips. Various concentrations of α2A/D- and α2C-adrenoceptor antagonists produced positive inotropic effect on ventricular strips and negative effect on atrial strips. α2B-Adrenoceptor blocker in the majority of the tested concentrations produced a positive inotropic effect in both atria and ventricles...
December 2, 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27909718/enzalutamide-inhibits-proliferation-of-gemcitabine-resistant-bladder-cancer-cells-with-increased-androgen-receptor-expression
#10
Koji Kameyama, Kengo Horie, Kosuke Mizutani, Taku Kato, Yasunori Fujita, Kyojiro Kawakami, Toshio Kojima, Tatsuhiko Miyazaki, Takashi Deguchi, Masafumi Ito
Advanced bladder cancer is treated mainly with gemcitabine and cisplatin, but most patients eventually become resistance. Androgen receptor (AR) signaling has been implicated in bladder cancer as well as other types of cancer including prostate cancer. In this study, we investigated the expression and role of AR in gemcitabine-resistant bladder cancer cells and also the potential of enzalutamide, an AR inhibitor, as a therapeutic for the chemoresistance. First of all, we established gemcitabine-resistant T24 cells (T24GR) from T24 bladder cancer cells and performed gene expression profiling...
November 24, 2016: International Journal of Oncology
https://www.readbyqxmd.com/read/27909497/persistent-atrial-fibrillation-and-atrial-flutter-complicated-by-tachycardiomyopathy-because-of-intermittent-conduction-through-accessory-pathway
#11
L Valeri, A Coppolino, G Rossetti, A Vado, G Amoroso, G Bricco, A Battisti, L Correndo, S Dogliani, A Magliarditi, D Pancaldo, M De Benedictis, A Bassignana, B Doronzo
The term tachycardiomyopathy refers to a specific form of tachycardia-related cardiomyopathy caused by supraventricular or ventricular tachyarrhytmias that are both associated with ventricular rates higher than 120 bpm. The arrhythmias which are most frequently associated with these forms of heart disease are atrial fibrillation and atrial flutter, particularly found in the elderly population. The most frequent clinical manifestation is heart failure. In this case we are reporting a clinical case of a patient that came to our attention because of an episode of heart failure associated with atrial fibrillation and atrial flutter...
April 2016: Journal of Atrial Fibrillation
https://www.readbyqxmd.com/read/27908709/surgical-intervention-for-oromandibular-dystonia-related-limited-mouth-opening-long-term-follow-up
#12
Kazuya Yoshida
BACKGROUND: Oromandibular dystonia is a movement disorder that is characterized by involuntary masticatory and/or lingual muscle contracture. Jaw closing dystonia, the most common subtype of this condition, can cause trismus and is frequently misdiagnosed as temporomandibular joint disorder or masticatory muscle tendon-aponeurosis hyperplasia. MATERIALS AND METHODS: This report describes the long-term results of 18 patients with limited mouth opening (mean maximal mouth opening: 12...
October 28, 2016: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/27908131/molecular-dynamics-investigation-of-desorption-and-ion-separation-following-picosecond-infrared-laser-pirl-ablation-of-an-ionic-aqueous-protein-solution
#13
J Zou, C Wu, W D Robertson, L V Zhigilei, R J D Miller
Molecular dynamics simulations were performed to characterize the ablation process induced by a picosecond infrared laser (PIRL) operating in the regime of desorption by impulsive vibrational excitation (DIVE) of a model peptide (lysozyme)/counter-ion system in aqueous solution. The simulations were performed for ablation under typical experimental conditions found within a time-of-flight mass spectrometer (TOF-MS), that is in vacuum with an applied electric field (E = ± 10(7) V/m), for up to 2 ns post-ablation and compared to the standard PIRL-DIVE ablation condition (E = 0 V/m)...
November 28, 2016: Journal of Chemical Physics
https://www.readbyqxmd.com/read/27907922/distinct-contributory-factors-determine-basophil-allergen-sensitivity-in-grass-pollen-rhinitis-and-in-anaphylactic-wasp-venom-allergy
#14
Peter Korošec, Mira Šilar, Peter Kopač, Renato Eržen, Mihaela Zidarn, Mitja Košnik
BACKGROUND: We sought to determine whether basophil-allergen sensitivity could be transferred to donor basophils by passive IgE sensitisation in allergic rhinitis and anaphylactic Hymenoptera venom hypersensitivity. METHODS: We studied 15 wasp venom-, 19 grass pollen- and 2 house dust mite-allergic patients, 2 healthy donors, and 8 wasp venom-allergic donors. In all subjects, we first evaluated the initial basophil response to wasp venom, grass pollen, or house dust mite allergen...
December 2, 2016: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/27906939/a-new-radiofrequency-ablation-procedure-to-treat-sacroiliac-joint-pain
#15
Jianguo Cheng, See Loong Chen, Nicole Zimmerman, Jarrod E Dalton, Garret LaSalle, Richard Rosenquist
BACKGROUND: Low back pain may arise from disorders of the sacroiliac joint in up to 30% of patients. Radiofrequency ablation (RFA) of the nerves innervating the sacroiliac joint has been shown to be a safe and efficacious strategy. OBJECTIVES: We aimed to develop a new RFA technique to relieve low back pain secondary to sacroiliac joint disorders. STUDY DESIGN: Methodology development with validation through prospective observational non-randomized trial (PONRT)...
November 2016: Pain Physician
https://www.readbyqxmd.com/read/27902761/tlr-2-recognizes-propionibacterium-acnes-camp-factor-1-from-highly-inflammatory-strains
#16
Coralie Lheure, Philippe Alain Grange, Guillaume Ollagnier, Philippe Morand, Nathalie Désiré, Sophie Sayon, Stéphane Corvec, Jöel Raingeaud, Anne-Geneviève Marcelin, Vincent Calvez, Amir Khammari, Frédéric Batteux, Brigitte Dréno, Nicolas Dupin
BACKGROUND: Propionibacterium acnes (P. acnes) is an anaerobic, Gram-positive bacteria encountered in inflammatory acne lesions, particularly in the pilosebaceous follicle. P. acnes triggers a strong immune response involving keratinocytes, sebocytes and monocytes, the target cells during acne development. Lipoteicoic acid and peptidoglycan induce the inflammatory reaction, but no P. acnes surface protein interacting with Toll-like receptors has been identified. P. acnes surface proteins have been extracted by lithium stripping and shown to induce CXCL8 production by keratinocytes...
2016: PloS One
https://www.readbyqxmd.com/read/27902239/mucilaginibacter-psychrotolerans-sp-nov-isolated-from-the-riganqiao-peatlands-on-the-tibetan-plateau
#17
Yongcui Deng, Liang Shen, Baiqin Xu, Yongqin Liu, Zhengquan Gu, Hongcan Liu, Yuguang Zhou
A Gram-stain-negative, rod-shaped without flagella, pink colored, cold-tolerate bacteria strain NH7-4T was isolated from Riganqiao peatlands on the Tibetan Plateau. The novel isolate was classified into the genus Mucilaginibacter. 16S rRNA gene sequence of the novel isolate shares a pairwise similarity ranging from 96.69% to 93.41% with the type strains of the genus Mucilaginibacter. Growth of strain NH7-4T occurred between 0 and 30 °C and at pH 5.0-9.0, with optimum at 20 °C and pH about 7.0. The major isoprenoid quinone is MK-7...
October 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27899699/a-smartphone-based-genotyping-method-for-hepatitis-b-virus-at-point-of-care-settings
#18
Huiqin Jiang, Di Wu, Liuwei Song, Quan Yuan, Shengxiang Ge, Xiaoping Min, Ningshao Xia, Shizhi Qian, Xianbo Qiu
We reported a rapid, convenient, and easy-to-use genotyping method for hepatitis B virus (HBV) based on the smartphone at point-of-care (POC) settings. To perform HBV genotyping especially for genotypes A, B, C, and D, a smartphone is used to image and analyze a one-step immunoassay lateral flow strip functionalized with genotype-specific monoclonal antibodies (mAbs) on multiple capture lines. A light-emitting diode (LED) positioned on the top of the lateral flow strip is used to shine the multiple capture lines for excitation...
November 29, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27898970/the-effect-of-branding-on-consumer-palatability-ratings-of-beef-strip-loin-steaks
#19
A K Wilfong, K V McKillip, J M Gonzalez, T A Houser, J A Unruh, E A E Boyle, T G O'Quinn
The objective of this study was to determine the influence of knowing the brand or USDA grade on consumer palatability ratings of beef strip loin steaks. Strip loins were selected to represent 5 USDA grades and brands, USDA Select, Choice, Prime, Certified Angus Beef (CAB; upper 2/3 Choice), and Select, from carcasses of cattle classified as Angus on the basis of phenotype. After 21 d of aging, 2.5-cm-thick steaks were cut, consecutively cut steaks were paired for consumer evaluation. Consumer panelists ( = 112) evaluated samples for tenderness, juiciness, flavor liking, and overall liking...
November 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898948/%C3%AE-and-%C3%AE-adrenergic-receptors-stimulation-relaxes-porcine-myometrium-in-the-peri-implantation-period
#20
W Markiewicz, J J Jaroszewski
These studies were to demonstrate whether the β-receptor is involved in regulating the contractility of the swine myometrium in the peri-implantation period and to compare a relaxing activity of selective β- and β-adrenergic agonists (salbutamol and BRL 37344, respectively). Myometrial strips were collected form the gilts ( = 8) on d 12 to 14 of pregnancy. Changes in the contractility of myometrial strips were assessed further to the administration of increasing concentrations of the agonists (10 to 10 ) with and without β-adrenergic receptor antagonists: butoxamine, propranolol, and bupranolol at 10 ...
November 2016: Journal of Animal Science
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