keyword
MENU ▼
Read by QxMD icon Read
search

Molecular biology exercise

keyword
https://www.readbyqxmd.com/read/28212624/understanding-the-response-to-endurance-exercise-using-a-systems-biology-approach-combining-blood-metabolomics-transcriptomics-and-mirnomics-in-horses
#1
Núria Mach, Yuliaxis Ramayo-Caldas, Allison Clark, Marco Moroldo, Céline Robert, Eric Barrey, Jesús Maria López, Laurence Le Moyec
BACKGROUND: Endurance exercise in horses requires adaptive processes involving physiological, biochemical, and cognitive-behavioral responses in an attempt to regain homeostasis. We hypothesized that the identification of the relationships between blood metabolome, transcriptome, and miRNome during endurance exercise in horses could provide significant insights into the molecular response to endurance exercise. For this reason, the serum metabolome and whole-blood transcriptome and miRNome data were obtained from ten horses before and after a 160 km endurance competition...
February 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28153015/the-effects-of-short-term-high-fat-feeding-on-exercise-capacity-multi-tissue-transcriptome-changes-by-rna-sequencing-analysis
#2
Ya Xiao, Wanshan Wang, Liguo Chen, Jieyu Chen, Pingping Jiang, Xiuqiong Fu, Xiaoli Nie, Hiuyee Kwan, Yanyan Liu, Xiaoshan Zhao
BACKGROUND: The effects of short-term high fat diets on physiology are elusive and the molecular changes following fat overconsumption remain largely unknown. In this study, we aimed to evaluate exercise capacity in mice fed with a high fat diet (HFD) for 3 days and investigate the molecular mechanisms in the early response to high-fat feeding. METHODS: Exercise capacity was assessed by weight-loaded swimming test in mice fed a control diet (10 kcal% fat) or a HFD (60 kcal% fat) for 3 days...
February 2, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28145107/a-comprehensive-experiment-for-molecular-biology-determination-of-single-nucleotide-polymorphism-in-human-rev3-gene-using-pcr-rflp
#3
Xu Zhang, Meng Shao, Lu Gao, Yuanyuan Zhao, Zixuan Sun, Liping Zhou, Yongmin Yan, Qixiang Shao, Wenrong Xu, Hui Qian
Laboratory exercise is helpful for medical students to understand the basic principles of molecular biology and to learn about the practical applications of molecular biology. We have designed a lab course on molecular biology about the determination of single nucleotide polymorphism (SNP) in human REV3 gene, the product of which is a subunit of DNA polymerase ζ and SNPs in this gene are associated with altered susceptibility to cancer. This newly designed experiment is composed of three parts, including genomic DNA extraction, gene amplification by PCR, and genotyping by RFLP...
February 1, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28111441/exercise-induced-upregulation-of-glutamate-cysteine-ligase-catalytic-subunit-gclc-and-glutamate-cysteine-ligase-modifier-subunit-gclm-gene-expression-in-thoroughbred-horses
#4
Jeong-Woong Park, Jae-Young Choi, Seul A Hong, Nam Young Kim, Kyoung-Tag Do, Ki-Duk Song, Byung-Wook Cho
Athletic ability is an important economic trait in horses, and molecular biological research on horses' athletic abilities can lead to improvements in the animals' economic value. In a previous study, we conducted RNA sequencing analyses of the blood and muscles of horses during exercise and identified differentially expressed genes (DEGs) in response to exercise. Among them, glutamate-cysteine ligase catalytic subunit (GCLC) and glutamate-cysteine ligase modifier subunit (GCLM) genes, of which products form glutamate cysteine ligase...
January 2, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28101273/who-scared-the-cat-a-molecular-crime-scene-investigation-laboratory-exercise
#5
Laura E Ott, Susan D Carson
This introductory laboratory exercise gives first-year life science majors or nonmajors an opportunity to gain knowledge and experience in basic bioinformatics and molecular biology laboratory techniques and analysis in the context of a mock crime scene investigation. In this laboratory, students determine if a human (Lady) or dog (Kona) committed the fictional crime of scaring a cat. Students begin by performing in silico PCR using provided dog- and human-specific PCR primers to determine the sequences to be amplified and predict PCR amplicon sizes...
December 2016: Journal of Microbiology & Biology Education: JMBE
https://www.readbyqxmd.com/read/28032403/a-unique-large-scale-undergraduate-research-experience-in-molecular-systems-biology-for-non-mathematics-majors
#6
Ulrike Kappler, Susan L Rowland, Rhianna K Pedwell
Systems biology is frequently taught with an emphasis on mathematical modeling approaches. This focus effectively excludes most biology, biochemistry, and molecular biology students, who are not mathematics majors. The mathematical focus can also present a misleading picture of systems biology, which is a multi-disciplinary pursuit requiring collaboration between biochemists, bioinformaticians, and mathematicians. This article describes an authentic large-scale undergraduate research experience (ALURE) in systems biology that incorporates proteomics, bacterial genomics, and bioinformatics in the one exercise...
December 28, 2016: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28027662/redox-control-of-skeletal-muscle-regeneration
#7
Emmeran Le Moal, Vincent Pialoux, Gaëtan Juban, Carole Groussard, Hassan Zouhal, Bénédicte Chazaud, Rémi Mounier
Skeletal muscle shows high plasticity in response to external demand. Moreover, adult skeletal muscle is capable of complete regeneration after injury, due to the properties of Muscle Stem Cells (MuSCs), the satellite cells, which follow a tightly regulated myogenic program to generate both new myofibers and new MuSCs for further needs. Whereas reactive oxygen species (ROS) and reactive nitrogen species (RNS) have long been associated with skeletal muscle physiology, their implication in the cell and molecular processes at work during muscle regeneration is more recent...
December 27, 2016: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/27941030/molecular-mechanisms-of-ros-production-and-oxidative-stress-in-diabetes
#8
REVIEW
Philip Newsholme, Vinicius Fernandes Cruzat, Kevin Noel Keane, Rodrigo Carlessi, Paulo Ivo Homem de Bittencourt
Oxidative stress and chronic inflammation are known to be associated with the development of metabolic diseases, including diabetes. Oxidative stress, an imbalance between oxidative and antioxidative systems of cells and tissues, is a result of over production of oxidative-free radicals and associated reactive oxygen species (ROS). One outcome of excessive levels of ROS is the modification of the structure and function of cellular proteins and lipids, leading to cellular dysfunction including impaired energy metabolism, altered cell signalling and cell cycle control, impaired cell transport mechanisms and overall dysfunctional biological activity, immune activation and inflammation...
December 15, 2016: Biochemical Journal
https://www.readbyqxmd.com/read/27939320/minion-as-part-of-a-biomedical-rapidly-deployable-laboratory
#9
Mathias C Walter, Katrin Zwirglmaier, Philipp Vette, Scott A Holowachuk, Kilian Stoecker, Gelimer H Genzel, Markus H Antwerpen
Fast turnaround times are of utmost importance for biomedical reconnaissance, particularly regarding dangerous pathogens. Recent advances in sequencing technology and its devices allow sequencing within a short time frame outside stationary laboratories close to the epicenter of the outbreak. In our study, we evaluated the portable sequencing device MinION as part of a rapidly deployable laboratory specialized in identification of highly pathogenic agents. We tested the device in the course of a NATO live agent exercise in a deployable field laboratory in hot climate conditions...
December 6, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27888580/biology-of-vo2-max-looking-under-the-physiology-lamp
#10
REVIEW
C Lundby, D Montero, M Joyner
In this review, we argue that several key features of maximal oxygen uptake (VO2 max) should underpin discussions about the biological and reductionist determinants of its interindividual variability: (i) training-induced increases in VO2 max are largely facilitated by expansion of red blood cell volume and an associated improvement in stroke volume, which also adapts independent of changes in red blood cell volume. These general concepts are also informed by cross-sectional studies in athletes that have very high values for VO2 max...
November 7, 2016: Acta Physiologica
https://www.readbyqxmd.com/read/27852752/temperature-induced-cardiac-remodelling-in-fish
#11
REVIEW
Adam N Keen, Jordan M Klaiman, Holly A Shiels, Todd E Gillis
Thermal acclimation causes the heart of some fish species to undergo significant remodelling. This includes changes in electrical activity, energy utilization and structural properties at the gross and molecular level of organization. The purpose of this Review is to summarize the current state of knowledge of temperature-induced structural remodelling in the fish ventricle across different levels of biological organization, and to examine how such changes result in the modification of the functional properties of the heart...
January 15, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/27848901/herb-medicines-against-osteoporosis-active-compounds-relevant-biological-mechanisms
#12
Lei Wu, Zhuoyan Ling, Xueqin Feng, Caiping Mao, Zhice Xu
Osteoporosis is one of common bone disorders, affecting millions of people worldwide. Treatments of osteoporosis consist of pharmacotherapy and non-pharmacological interventions, such as mineral supplementation, lifestyle changes, and exercise programs. Due to the minimum side effects and favorable cost-effective therapeutic effects, herbal medicine has been widely applied in clinical practices for more than 2,000 years in China. Of the many traditional formulas reported for treating bone diseases, 4 single herbs namely (1) Herba Epimedii, (2) Rhizoma Drynariae, (3) Fructus Psoraleae, and (4) Cortex Eucommiae, are considered as the featured "Kidney-Yang" tonics, and frequently and effectively applied for preventing and treating osteoporosis...
November 16, 2016: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/27833825/epidemiology-pathogenesis-and-diagnosis-of-lymphangioleiomyomatosis
#13
Angelo M Taveira-DaSilva, Joel Moss
INTRODUCTION: Lymphangioleiomyomatosis (LAM) is a disease of women characterized by cystic lung destruction, lymphatic involvement, and renal angiomyolipomas. AREAS COVERED: LAM is caused by proliferation of abnormal smooth muscle-like LAM cells containing mutations and perhaps epigenetic modifications of the TSC1 or TSC2 genes, which encode, respectively, hamartin and tuberin, two proteins controlling the mechanistic target of rapamycin (mTOR) signaling pathway...
2016: Expert Opinion on Orphan Drugs
https://www.readbyqxmd.com/read/27790107/do-dopaminergic-impairments-underlie-physical-inactivity-in-people-with-obesity
#14
REVIEW
Alexxai V Kravitz, Timothy J O'Neal, Danielle M Friend
Obesity is associated with physical inactivity, which exacerbates the negative health consequences of obesity. Despite a wide consensus that people with obesity should exercise more, there are few effective methods for increasing physical activity in people with obesity. This lack is reflected in our limited understanding of the cellular and molecular causes of physical inactivity in obesity. We hypothesize that impairments in dopamine signaling contribute to physical inactivity in people with obesity, as in classic movement disorders such as Parkinson's disease...
2016: Frontiers in Human Neuroscience
https://www.readbyqxmd.com/read/27711947/-opportunities-and-challenges-of-extrapolation-for-biosimilars
#15
M Weise, E Wolff-Holz
Although biosimilars approved in the European Union have proved to be safe and efficacious, their licensing requirements continue to be disputed by medical professionals. In particular, extrapolation to indications of the originator without one's own clinical data of the biosimilar is controversial. Conceptually, the development of biosimilars is derived from that of generics. However, due to their complexity and inherent variability, considerably more data are necessary for biosimilars to demonstrate comparability with the originator (the reference product) than for the usually low-molecular generics...
November 2016: Zeitschrift Für Gastroenterologie
https://www.readbyqxmd.com/read/27677251/discovery-of-escherichia-coli-crispr-sequences-in-an-undergraduate-laboratory
#16
Kevin T Militello, Justine C Lazatin
Clustered regularly interspaced short palindromic repeats (CRISPRs) represent a novel type of adaptive immune system found in eubacteria and archaebacteria. CRISPRs have recently generated a lot of attention due to their unique ability to catalog foreign nucleic acids, their ability to destroy foreign nucleic acids in a mechanism that shares some similarity to RNA interference, and the ability to utilize reconstituted CRISPR systems for genome editing in numerous organisms. In order to introduce CRISPR biology into an undergraduate upper-level laboratory, a five-week set of exercises was designed to allow students to examine the CRISPR status of uncharacterized Escherichia coli strains and to allow the discovery of new repeats and spacers...
September 28, 2016: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27666582/introducing-the-concept-of-biocatalysis-in-the-classroom-the-conversion-of-cholesterol-to-provitamin-d3
#17
Belén M De Luca, Clara B Nudel, Rodrigo H Gonzalez, Alejandro D Nusblat
Biocatalysis is a fundamental concept in biotechnology. The topic integrates knowledge of several disciplines; therefore, it was included in the course "design and optimization of biological systems" which is offered in the biochemistry curricula. We selected the ciliate tetrahymena as an example of a eukaryotic system with potential for the biotransformation of sterol metabolites of industrial interest; in particular, we focused on the conversion of cholesterol to provitamin D3. The students work with wild type and recombinant strains and learn how sterol pathways could be modified to obtain diverse sterol moieties...
September 26, 2016: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27664113/an-exercise-in-extrapolation-clinical-management-of-atypical-cml-mds-mpn-unclassifiable-and-mds-mpn-rs-t
#18
REVIEW
Chetasi Talati, Eric Padron
According to the recently published 2016 World Health Organization (WHO) classification of myeloid malignancies, myelodysplastic/myeloproliferative neoplasms (MDS/MPN) include atypical chronic myeloid leukemia (aCML), MDS/MPN-unclassifiable (MDS/MPN-U), chronic myelomonocytic leukemia (CMML), juvenile myelomonocytic leukemia (JMML), and MDS/MPN ring sideroblasts with thrombocytosis (MDS/MPN-RS-T). MDS/MPN-RS-T was previously a provisional category known as refractory anemia with ring sideroblasts with thrombocytosis (RARS-T) which has now attained a distinct designation in the 2016 WHO classification...
December 2016: Current Hematologic Malignancy Reports
https://www.readbyqxmd.com/read/27622493/molecular-biomarkers-to-assess-health-risks-due-to-environmental-contaminants-exposure
#19
Irais Poblete-Naredo, Arnulfo Albores
Biomarkers, or bioindicators, are metric tools that, when compared with reference values, allow specialists to perform risk assessments and provide objective information to decision makers to design effective strategies to solve health or environmental problems by efficiently using the resources assigned. Health risk assessment is a multidisciplinary exercise, and molecular biology is a discipline that greatly contributes to these evaluations because the genome, transcriptome, proteome and metabolome could be affected by xenobiotics causing measurable changes that might be useful biomarkers...
June 3, 2016: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/27555311/a-full-length-infectious-cdna-clone-of-zika-virus-from-the-2015-epidemic-in-brazil-as-a-genetic-platform-for-studies-of-virus-host-interactions-and-vaccine-development
#20
Konstantin A Tsetsarkin, Heather Kenney, Rubing Chen, Guangping Liu, Hasmik Manukyan, Stephen S Whitehead, Majid Laassri, Konstantin Chumakov, Alexander G Pletnev
UNLABELLED: An arthropod-borne virus, Zika virus (ZIKV), has recently emerged as a major human pathogen. Associated with complications during perinatal development and Guillain-Barré syndrome in adults, ZIKV raises new challenges for understanding the molecular determinants of flavivirus pathogenesis. This underscores the necessity for the development of a reverse genetic system based on an epidemic ZIKV strain. Here, we describe the generation and characterization in cell cultures of an infectious cDNA clone of ZIKV isolated from the 2015 epidemic in Brazil...
August 23, 2016: MBio
keyword
keyword
62025
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"