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Helena J Chapman, Luisa A Armas-Pérez, Michael Lauzardo, Edilberto R González-Ochoa
Global efforts to eliminate tuberculosis by 2050 continue to challenge health systems. In countries with low reported tuberculosis incidence, such as the USA (3.1/100,000 population) and Cuba (6.9/100,000), differences in classification by income level and health systems' overall organizational structure are evident. However, the two countries' low tuberculosis incidence, geographic proximity, robust research capacity and shared health priority for tuberculosis elimination provide fertile territory to strengthen collaboration for tuberculosis control in clinical, laboratory and community settings...
April 2018: MEDICC Review
Roberto F Casal, Garrett Walsh, Mark McArthur, Lori R Hill, Maria F Landaeta, Aristides J Armas Villalba, Philip Ong, Labib Debiane, Erik Vakil, Horiana B Grosu, Carlos A Jimenez, David Ost, Roberto Adachi, George A Eapen
BACKGROUND: Population aging and lung cancer screening strategies may lead to an increase in detection of early-stage lung cancer in medical inoperable patients. Recent advances in peripheral bronchoscopy have made it a suitable platform for ablation of small peripheral tumors. METHODS: We investigated the tissue-ablative effect of a diode laser bronchoscopically applied by a laser delivery fiber (LDF) with wide aperture on porcine lung parenchyma. Laser was tested ex vivo and in vivo to identify the most effective power settings and LDF...
May 16, 2018: Journal of Bronchology & Interventional Pulmonology
Pietro Fratta, Prasanth Sivakumar, Jack Humphrey, Kitty Lo, Thomas Ricketts, Hugo Oliveira, Jose M Brito-Armas, Bernadett Kalmar, Agnieszka Ule, Yichao Yu, Nicol Birsa, Cristian Bodo, Toby Collins, Alexander E Conicella, Alan Mejia Maza, Alessandro Marrero-Gagliardi, Michelle Stewart, Joffrey Mianne, Silvia Corrochano, Warren Emmett, Gemma Codner, Michael Groves, Ryutaro Fukumura, Yoichi Gondo, Mark Lythgoe, Erwin Pauws, Emma Peskett, Philip Stanier, Lydia Teboul, Martina Hallegger, Andrea Calvo, Adriano Chiò, Adrian M Isaacs, Nicolas L Fawzi, Eric Wang, David E Housman, Francisco Baralle, Linda Greensmith, Emanuele Buratti, Vincent Plagnol, Elizabeth Mc Fisher, Abraham Acevedo-Arozena
TDP-43 (encoded by the gene TARDBP ) is an RNA binding protein central to the pathogenesis of amyotrophic lateral sclerosis (ALS). However, how TARDBP mutations trigger pathogenesis remains unknown. Here, we use novel mouse mutants carrying point mutations in endogenous Tardbp to dissect TDP-43 function at physiological levels both in vitro and in vivo Interestingly, we find that mutations within the C-terminal domain of TDP-43 lead to a gain of splicing function. Using two different strains, we are able to separate TDP-43 loss- and gain-of-function effects...
May 15, 2018: EMBO Journal
Pablo Armas, Nora Beatriz Calcaterra
During animal development, gene expression is orchestrated by specific and highly evolutionarily conserved mechanisms that take place accurately, both at spatial and temporal levels. The last decades have provided compelling evidence showing that chromatin state plays essential roles in orchestrating most of the stages of development. The DNA molecule can adopt alternative structures different from the helical duplex architecture. G-rich DNA sequences can fold as intrastrand quadruple helix structures called G-quadruplexes or G4-DNA...
May 11, 2018: Mechanisms of Development
Elena Sánchez-Elordi, Roberto Contreras, Roberto de Armas, Mario C Benito, Borja Alarcón, Eliandre de Oliveira, Carlos Del Mazo, Eva M Díaz-Peña, Rocío Santiago, Carlos Vicente, María E Legaz
Proteomic profiling of the stalk of a smut resistant and a susceptible sugarcane cultivars revealed the presence of dirigent and dirigent-like proteins in abundance in the pool of high molecular mass (HMMG) and mid-molecular mass (MMMG) glycoproteins, produced as part of the defensive response to the fungal smut pathogen. Quantitative RT-PCR analysis showed that expression levels of SofDIR16 (sugarcane dirigent16) and SofCAD (sugarcane cinnamyl alcohol dehydrogenase) were higher in the smut resistant My 55-14 cultivar than in the sensitive B 42231 cultivar prior to infection...
May 4, 2018: Journal of Plant Physiology
Supriya Upadhyay, Annie Bakorlin Khyriem, Prithwis Bhattacharya, Amitabha Bhattacharjee, Santa Ram Joshi
Background: Acinetobacter baumannii has emerged as an important nosocomial pathogen, its ability to acquire resistance to carbapenems and aminoglycosides, has complicated their treatment regimen. The present study investigates the prevalence and diversity of aminoglycoside-modifying enzymes and 16S methyltransferases in A. baumannii isolates recovered from patients admitted in Intensive Care Unit (ICU) of a tertiary referral hospital in Northeastern India. Materials and Methods: We investigated the high-level aminoglycoside-resistance (HLAR) (gentamicin and amikacin minimum inhibitory concentration ≥ 512 μg/ml) among 164 multidrug-resistant A...
January 2018: Indian Journal of Medical Microbiology
Marta Fernández-Martínez, Belén Ruiz Del Castillo, Maria Jesús Lecea-Cuello, Jesús Rodríguez-Baño, Álvaro Pascual, Luis Martínez-Martínez
The in vitro activity of amikacin, gentamicin, kanamycin, tobramycin, neomycin, and netilmicin against 420 Escherichia coli producing extended spectrum β-lactamases (Ec-ESBLs) and 139 Klebsiella pneumoniae producing extended spectrum β-lactamase (Kp-ESBL) collected in two multicenter studies performed in Spain in 2000 and 2006 was determined. The presence of genes encoding aminoglycoside-modifying enzymes (AMEs) and 16S ribosomal RNA (rRNA) methylases [aac(3)-Ia, aac(3)-IIa, aac(3)-IVa, aac(6')-Ib, ant(2")-Ia, ant(4')-IIa, aph(3')-Ia, aph(3')-IIa, armA, rmtB, and rmtC] was also investigated...
May 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Monika Garrido-Armas, Juan Carlos Corona, Maria Luisa Escobar, Leda Torres, Francisco Ordóñez-Romero, Abrahan Hernández-Hernández, Francisco Arenas-Huertero
Curcumin is a polyphenol compound extracted from Curcuma longa plant, is a molecule with pleiotropic effects that suppresses transformation, proliferation and metastasis of malignant tumors. Curcumin can cause different kinds of cell death depending of its concentration on the exposed cell type. Here we show that exposure of the glioblastoma cell line A172 to curcumin at 50 μM, the IC50, causes morphological changes characteristic of paraptosis cell-death. Vesicles derived from the ER and low membrane potential of the mitochondria were constantly found in the exposed cells...
April 30, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Robin W M Vernooij, Laura Martínez García, Ivan Dario Florez, Laura Hidalgo Armas, Michiel H F Poorthuis, Melissa Brouwers, Pablo Alonso-Coello
After publication of the original article [1] it was brought to our attention that author Laura Hidalgo Armas was incorrectly included as Laura Hildago Armas.
April 30, 2018: Implementation Science: IS
Amr Sonousi, Vikram A Sarpe, Margarita Brilkova, Jochen Schacht, Andrea Vasella, Erik C Böttger, David Crich
Syntheses of the 6'-N-(2-hydroxyethyl) and 1-N-(4-amino-2S-hydroxybutyryl) derivatives of the 4,6-aminoglycoside sisomicin and that of the doubly modified 1-N-(4-amino-2S-hydroxybutyryl)-6'-N-(2-hydroxyethyl) derivative known as plazomicin are reported together with their antibacterial and antiribosomal activities and selectivities. The 6'-N-(2-hydroxyethyl) modification results in a moderate increase in prokaryotic/eukaryotic ribosomal selectivity, whereas the 1-N-(4-amino-2S-hydroxybutyryl) modification has the opposite effect...
April 30, 2018: ACS Infectious Diseases
Aisel Valle, Luis Benito Pérez-Socas, Liem Canet, Yadira de la Patria Hervis, German de Armas-Guitart, Diogo Martins-de-Sa, Jônatas Cunha Barbosa Lima, Adolfo Carlos Barros Souza, João Alexandre Ribeiro Gonçalves Barbosa, Sonia Maria de Freitas, Isabel Fabiola Pazos
The Trp111 to Cys mutant of sticholysin I, an actinoporin from Stichodactyla helianthus sea anemone, forms a homodimer via a disulfide bridge. The purified dimer is 193 times less hemolytic than the monomer. Ultracentrifugation, dynamic light scattering and size-exclusion chromatography demonstrate that monomers and dimers are the only independent oligomeric states encountered. Indeed, circular dichroism and fluorescence spectroscopies showed that Trp/Tyr residues participate in homodimerization and that the dimer is less thermostable than the monomer...
April 26, 2018: Scientific Reports
Varghese K George, Suresh Pallikkuth, Rajendra Pahwa, Lesley de Armas, Stefano Rinaldi, Li Pan, Savita Pahwa
OBJECTIVE: Antibody responses are often impaired in old age and in HIV+ infection despite virologic control with antiretroviral therapy but innate immunologic determinants are not well understood. DESIGN: Monocytes and natural killer cells were examined for relationships to age, HIV infection and influenza vaccine responses. METHODS: Virologically suppressed HIV+ (n = 139) and HIV- (n = 137) participants classified by age as young (18-39 years), middle-aged (40-59 years) and old (≥60 years) were evaluated preinfluenza and postinfluenza vaccination...
April 19, 2018: AIDS
Azahara Luna-Triguero, Jose Manuel Vicent-Luna, Ali Poursaeidesfahani, Thijs J H Vlugt, Rocio Sánchez-de-Armas, Paula Gómez-Álvarez, Sofia Calero
The separation and purification of light hydrocarbons is challenging in industry. Recently, ZJNU-30 metal-organic framework has been found potential for adsorption-based separation of olefins and diolefins with four carbon atoms [H. M. Liu et al. Chem. - Eur. J. 2016, 22, 14988-14997]. Our study corroborates this finding but reveals Fe-MOF-74 as a more efficient candidate for the separation due the open metal sites. We performed adsorption-base separation, transient breakthrough curves, and density functional theory calculations...
April 19, 2018: ACS Applied Materials & Interfaces
Elaa Maamar, Carla Andrea Alonso, Sana Ferjani, Ali Jendoubi, Zaineb Hamzaoui, Alia Jebri, Mabrouka Saidani, Salma Ghedira, Carmen Torres, Ilhem Boutiba-Ben Boubaker
Gastrointestinal colonization by carbapenem-resistant Acinetobacter baumannii (CRAB), is a critical step before nosocomial infection. Our objective was to evaluate CRAB intestinal carriage in patients admitted in the intensive care unit (ICU) of a Tunisian Hospital and to determine the antimicrobial resistance mechanisms involved. From December-2014 to February-2015, all 63 patients admitted at the ICU were screened for rectal CRAB-colonization upon admission and once weekly thereafter. ICU-patients who acquired a CRAB-nosocomial-infection were also included...
April 14, 2018: International Journal of Antimicrobial Agents
Claire Hennequin, Viviane Ravet, Frédéric Robin
The aim of this review is to provide an update on the plasmids mediating DHA-1 cephalosporinase in Klebsiella pneumoniae. These plasmids have been mainly found in this bacterium but not only. The first was isolated from Salmonella sp. in France in the early 1990s. They are currently reported worldwide. BlaDHA-1 beta-lactamase gene is usually co-expressed with many other antibiotic resistance genes such as extended-spectrum β-lactamases (blaCTX-M -, bla SHV -types), oxacillinases (blaOXA-1, blaOXA-30 ), penicillinases (bla TEM -type), carbapenemases (bla OXA48 , blaKPC-2 ), aminoglycosides (aacA, aadA, armA), fluoroquinolones (qnrB4, aac6'-1b-cr), and sulfonamide (sul1) resistance genes...
April 17, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Bruna Parreira, João C R Cardoso, Rita Costa, Ana Rita Couto, Jácome Bruges-Armas, Deborah M Power
The ATP-binding cassette transporter 6 (ABCC6) gene encodes a cellular transmembrane protein transporter (MRP6) that is involved in the regulation of tissue calcification in mammals. Mutations in ABCC6 are associated with human ectopic calcification disorders. To gain insight into its evolution and involvement in tissue calcification we conducted a comparative analysis of the ABCC6 gene and the related gene ABCC1 from invertebrates to vertebrates where a bony endoskeleton first evolved. Taking into consideration the role of ABCC6 in ectopic calcification of human skin we analysed the involvement of both genes in the regeneration of scales, mineralized structures that develop in fish skin...
April 16, 2018: Scientific Reports
Flávia Thiebaut, Cristian A Rojas, Clícia Grativol, Edmundo P da R Calixto, Mariana R Motta, Helkin G F Ballesteros, Barbara Peixoto, Berenice N S de Lima, Lucas M Vieira, Maria Emilia Walter, Elvismary M de Armas, Júlio O P Entenza, Sergio Lifschitz, Laurent Farinelli, Adriana S Hemerly, Paulo C G Ferreira
Studies have highlighted the importance of non-coding RNA regulation in plant-microbe interaction. However, the roles of sugarcane microRNAs (miRNAs) in the regulation of disease responses have not been investigated. Firstly, we screened the sRNA transcriptome of sugarcane infected with Acidovorax avenae . Conserved and novel miRNAs were identified. Additionally, small interfering RNAs (siRNAs) were aligned to differentially expressed sequences from the sugarcane transcriptome. Interestingly, many siRNAs aligned to a transcript encoding a copper-transporter gene whose expression was induced in the presence of A...
December 20, 2017: Non-Coding RNA
Arezoo Saadatian Farivar, Jamileh Nowroozi, Gita Eslami, Azar Sabokbar
The increasing prevalence of multidrug-resistant Klebsiella pneumoniae strains isolated from hospitals shows the limitation of recent antibiotics used for bacterial eradication. In this study, 81 K. pneumoniae isolates were collected from three hospitals in Tehran. Antibiotic susceptibility test showed the highest rates of resistance to cefotaxim (85.5%) and ceftazidime (78.3%), and the lowest rates of resistance were detected for colistin (16.9%), streptomycin (16.8%), and chloroamphenicol (21.7%). Eleven different resistance patterns were observed...
2018: Canadian Journal of Infectious Diseases & Medical Microbiology
Emma Taylor, Shiranee Sriskandan, Neil Woodford, Katie L Hopkins
The emergence of 16S rRNA methyltransferases (16S RMTases) worldwide is a growing concern due to their ability to confer high-level resistance (MICs >256 mg/L) to all clinically-relevant aminoglycosides. As the occurrence of 16S RMTases in the United Kingdom has not been investigated to date, we screened 806 Enterobacteriaceae isolates displaying high-level aminoglycoside resistance (amikacin, gentamicin and tobramycin MICs ≥64, ≥32 and ≥32 mg/L, respectively) for 16S RMTases either by analysing whole-genome sequence (WGS) data (which were available for 449 isolates) or by PCR...
March 26, 2018: International Journal of Antimicrobial Agents
Ismael Gárate-Lizárraga, Rogelio González-Armas, Yuri B Okolodkov
From December 2016 to May 2017, 22 phytoplankton surface samples were collected with a 20-μm mesh net at three islands of the Archipiélago de Revillagigedo (Partida, Socorro and San Benedicto), Mexican Pacific. The sites depth was approximately 20-80 m; the surface water temperature was 21-27 °C. The potentially toxic benthic dinoflagellate Ostreopsis lenticularis was present in all the samples. Cells had a lenticular to broadly oval shape, 65-100 μm long and 50-80 μm wide. The taxonomy of the genus Ostreopsis has been unclear due to equivocal ascribing some taxonomic features among species...
March 2018: Marine Pollution Bulletin
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