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https://www.readbyqxmd.com/read/28771142/comparison-of-semi-automated-commercial-rep-pcr-fingerprinting-spoligotyping-12-locus-miru-vntr-typing-and-single-nucleotide-polymorphism-analysis-of-the-embb-gene-as-molecular-typing-tools-for-mycobacterium-bovis
#1
Federica Armas, Cristina Camperio, Luana Coltella, Serena Selvaggini, Maria Beatrice Boniotti, Maria Lodovica Pacciarini, Vincenzo Di Marco Lo Presti, Cinzia Marianelli
PURPOSE: Highly discriminatory genotyping strategies are essential in molecular epidemiological studies of tuberculosis. In this study we evaluated, for the first time, the efficacy of the repetitive sequence-based PCR (rep-PCR) DiversiLab Mycobacterium typing kit over spoligotyping, 12-locus mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing and embB single nucleotide polymorphism (SNP) analysis for Mycobacterium bovis typing. METHODOLOGY: A total of 49 M...
August 4, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28765517/the-hedgehog-gli-pathway-in-embryonic-development-and-cancer-implications-for-pulmonary-oncology-therapy
#2
REVIEW
Armas López Leonel, Zúñiga Joaquín, Arrieta Oscar, Ávila-Moreno Federico
Transcriptional regulation and epigenetic mechanisms closely control gene expression through diverse physiological and pathophysiological processes. These include the development of germ layers and post-natal epithelial cell-tissue differentiation, as well as, involved with the induction, promotion and/or progression of human malignancies.Diverse studies have shed light on the molecular similarities and differences involved in the stages of embryological epithelial development and dedifferentiation processes in malignant tumors of epithelial origin, of which many focus on lung carcinomas...
July 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28763663/prediction-of-fish-catch-in-the-danube-river-based-on-long-term-variability-in-environmental-parameters-and-catch-statistics
#3
Marija M Smederevac-Lalić, Aleksandar J Kalauzi, Slobodan B Regner, Mirjana B Lenhardt, Zorana Z Naunovic, Aleksandar E Hegediš
The effects of physical factors on fish catch in the Serbian part of the Danube River were studied for period of six decades. The data on total catch for the Danube River from river kilometre 845 to river kilometre 1433 were collected from Statistical Office of the Republic of Serbia, while water level and water temperature data were collected from 16 water gauge stations along the investigated part of the Danube River for the period 1948-2009. Cross-correlation functions have been used to analyse the functional connection between Danube water level, water temperature and fish catch while ARMA model which combines cyclic (deterministic) and random (stochastic) components of the analysed sequences was used for the forecasts...
July 28, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28755740/vascular-damage-and-kidney-transplant-outcomes-an-unfriendly-and-harmful-link
#4
REVIEW
Domingo Hernández, Javier Triñanes, Ana María Armas, Pedro Ruiz-Esteban, Juana Alonso-Titos, Ana Duarte, Miguel González-Molina, Eulalia Palma, Eduardo Salido, Armando Torres
Kidney transplant (KT) is the treatment of choice for most patients with chronic kidney disease, but this has a high cardiovascular mortality due to traditional and nontraditional risk factors, including vascular calcification. Inflammation could precede the appearance of artery wall lesions, leading to arteriosclerosis and clinical and subclinical atherosclerosis in these patients. Additionally, mineral metabolism disorders and activation of the renin-angiotensin system could contribute to this vascular damage...
July 2017: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/28754338/unexpected-diagnosis-of-renal-cell-carcinoma-after-thyroid-nodule-study
#5
José Antonio Casimiro Pérez, Desiré Armas Ojeda, María Asunción Acosta Mérida, Carlos Fernández Quesada, Joaquín Marchena Gómez
No abstract text is available yet for this article.
July 25, 2017: Medicina Clínica
https://www.readbyqxmd.com/read/28736573/a-rapid-and-efficient-in-vitro-regeneration-system-for-lettuce-lactuca-sativa-l
#6
Isabel Armas, Natalia Pogrebnyak, Ilya Raskin
BACKGROUND: Successful biotechnological improvement of crop plants requires a reliable and efficient in vitro regeneration system. Lettuce (Lactuca sativa L.), one the most important vegetable crops worldwide, is strongly genotype-dependent in terms of regeneration capacity, limiting the potential for biotechnological improvement of cultivars which show recalcitrance under currently available protocols. The effect of different nutrient sources, plant hormone combinations and activated charcoal supplementation on shoot induction efficiency was evaluated on the cultivar 'RSL NFR', which had previously shown poor regeneration efficiency...
2017: Plant Methods
https://www.readbyqxmd.com/read/28735317/genetic-characterization-of-a-novel-composite-transposon-carrying-arma-and-aac-6-ib-genes-in-an-escherichia-coli-isolate-from-egypt
#7
Mona T Kashef, Omneya M Helmy
Aminoglycosides are used in treating a wide range of infections caused by Gram-positive and Gram-negative bacteria; however, aminoglycoside resistance is common and occurs by several mechanisms. Among these mechanisms is bacterial rRNA methylation by the 16S rRNA methyl transferase (16S-RMTase) enzymes; but data about the spread of this mechanism in Egypt are scarce. Cephalosporins are the most commonly used antimicrobial agents in Egypt; therefore, this study was conducted to determine the frequency of 16S-RMTase among third generation cephalosporin-resistant clinical isolates in Egypt...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28733214/strategies-to-improve-the-efficiency-of-celiac-disease-diagnosis-in-the-laboratory
#8
Delia Almeida González, Laura García de Armas, Itahisa Marcelino Rodríguez, Ana Arencibia Almeida, Miriam García García, Fadoua Gannar, Antonio Cabrera de León
The demand for testing to detect celiac disease (CD) autoantibodies has increased, together with the cost per case diagnosed, resulting in the adoption of measures to restrict laboratory testing. We designed this study to determine whether opportunistic screening to detect CD-associated autoantibodies had advantages compared to efforts to restrict testing, and to identify the most cost-effective diagnostic strategy. We compared a group of 1678 patients in which autoantibody testing was restricted to cases in which the test referral was considered appropriate (G1) to a group of 2140 patients in which test referrals were not reviewed or restricted (G2)...
July 18, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28732788/occurrence-and-clonal-diversity-of-multidrug-resistant-klebsiella-pneumoniae-recovered-from-inanimate-surfaces-in-algerian-hospital-environment-first-report-of-arma-qnrb-and-aac-6-ib-cr-genes
#9
Karima Zenati, Farida Sahli, Vincent Garcia, Sofiane Bakour, Djellali Belhadi, Jean Marc Rolain, Abdelaziz Touati
PURPOSE: The aim of this study is to characterize the molecular support of antibiotic resistance in MDR Klebsiella pneumoniae recovered from inanimate surfaces between March 2012 to February 2014 in three teaching hospitals (Setif, Bejaia and Constantine) in Algeria. RESULTS: Forty-four K. pneumoniae producing ESBL were detected and blaCTX-M-15 and blaCTX-M-3 were detected respectively in 41 and 3 isolates. These K. pneumoniae isolates producing ESBL were also resistant to gentamicin (87%), tobramicin (87%), ciprofloxacin (66%) and ofloxacin (62%)...
July 19, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28711582/a-novel-wnt-pathway-inhibitor-sm04690-for-the-treatment-of-moderate-to-severe-osteoarthritis-of-the-knee-results-of-a-24-week-randomized-controlled-phase-1-study
#10
Y Yazici, T E McAlindon, R Fleischmann, A Gibofsky, N E Lane, A J Kivitz, N Skrepnik, E Armas, C J Swearingen, A DiFrancesco, J R S Tambiah, J Hood, M C Hochberg
OBJECTIVE: To assess the safety, pharmacokinetics, and exploratory efficacy of SM04690, a novel Wnt pathway inhibitor, as a potential disease modifying treatment for knee osteoarthritis (OA). DESIGN: Subjects with Kellgren-Lawrence grade 2-3 knee OA were randomized in successive dose-escalation cohorts to receive a knee intra-articular (IA) injection with 0.03, 0.07, or 0.23 mg SM04690, or placebo (PBO) (4:1 ratio). Safety, pharmacokinetics, efficacy (WOMAC Total/Function/Pain, Pain VAS, Physician Global Assessment [MDGA], and OMERACT-OARSI Response), OA-related biomarker (P1NP, ß-CTX, and cartilage oligomeric matrix protein [COMP]), and radiographic/imaging data were collected at baseline and during 24-week follow-up...
July 13, 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/28701599/interesting-images-multiple-coronary-artery-aneurysms
#11
Jonathon M Howard, Omar Viswanath, Alfredo Armas, Orlando Santana, Gerald P Rosen
We present the case of a 65-year-old male who presented with stable angina and dyspnea on exertion. His initial workup yielded a positive treadmill stress test for reversible apical ischemia, and transthoracic echocardiogram demonstrated impaired systolic function. Cardiac catheterization was then performed, revealing severe atherosclerotic disease including multiple coronary artery aneurysms. As a result, the patient was advised to and subsequently underwent a coronary artery bypass graft. This case highlights the presence of multiple coronary artery aneurysms and the ability to appreciate these pathologic findings on multiple imaging modalities, including coronary angiogram, transesophageal echocardiography, and direct visualization through the surgical field...
July 2017: Annals of Cardiac Anaesthesia
https://www.readbyqxmd.com/read/28699154/-venezuelan-consensus-guidelines-on-the-use-of-magnetic-resonance-in-diagnosis-and-follow-up-of-patients-with-multiple-sclerosis
#12
B Lander, M Alvarado, I C Alvarez, E Armas, G Chique-Alfonzo, F Hernandez, R Labarca, R Leon, O Molina, I Monasterios, C I Ramirez, E Rubio, B Torres, R Viso-Barroso, Z Simmonds, A Soto
INTRODUCTION: The clinical use of magnetic resonance (MR) in patients with multiple sclerosis (MS) has advanced markedly over the past few years. Several groups around the world have developed consensus guidelines about the role of MR in MS at diagnosis and during follow up. However, in some regions is difficult to extrapolate the recommendations. AIM: To provide recommendations for the implementation of MR in MS patients at diagnosis and follow up in Venezuela...
August 1, 2017: Revista de Neurologia
https://www.readbyqxmd.com/read/28681822/emergence-of-rmtc-and-rmtf-16s-rrna-methyltransferase-in-clinical-isolates-of-pseudomonas-aeruginosa
#13
Lavanya Mohanam, Thangam Menon
Occurrence of aminoglycoside (AG) resistance in clinical isolates of Pseudomonas aeruginosa is investigated in this study. Antimicrobial susceptibility test and minimum inhibitory concentration (MIC) for amikacin and gentamicin were performed followed by polymerase chain reaction amplifications of AG modifying enzyme genes (aac(6´)-I, aac(6´)-II, aac(3)-II/VI, ant(2´´)-I, aph(3´)-VI) and 16S methylases (rmtA-D, rmtF and armA). MIC50and MIC90were 64, 128 and > 256, >256 for amikacin and gentamicin, respectively...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28668692/wastewater-is-a-reservoir-for-clinically-relevant-carbapenemase-and-16s-rrna-methylase-producing-enterobacteriaceae
#14
Katrin Zurfluh, Claudia Bagutti, Peter Brodmann, Monica Alt, Jürg Schulze, Séamus Fanning, Roger Stephan, Magdalena Nüesch-Inderbinen
The aim of the study was to evaluate wastewater for carbapenemase producing Enterobacteriaceae (CPE) and 16S rRNA methylase producing Gram negative bacteria (MPB), and to assess their occurrence following wastewater treatment. Wastewater samples were collected between June 2015 and March 2016 in the sewage network of the city of Basel from sites located before and after influx of wastewater from the hospital into the sewage network. Samples were also obtained from the influent and from the effluent of the receiving wastewater treatment plant...
June 28, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28666753/first-report-of-an-escherichia-coli-strain-from-swine-carrying-an-oxa-181-carbapenemase-and-the-colistin-resistance-determinant-mcr-1
#15
Sandra Pulss, Torsten Semmler, Ellen Prenger-Berninghoff, Rolf Bauerfeind, Christa Ewers
Plasmid-mediated resistance to carbapenems and colistin in Enterobacteriaceae represents an emerging public health threat. Although animals have been identified as a relevant source of multidrug-resistant (MDR) bacteria, there are only a few reports on the presence of carbapenemases in animal isolates. In this study, 7850 faecal Escherichia coli isolates obtained from 2160 pigs were screened for carbapenem non-susceptibility using Mueller-Hinton agar supplemented with meropenem. Eleven isolates showed growth on meropenem-containing agar but only two proved positive by PCR for a carbapenemase gene, namely blaOXA-48-like...
June 27, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28639934/first-description-of-methyltransferases-in-extensively-drug-resistant-klebsiella-pneumoniae-isolates-from-saudi-arabia
#16
Baha Abdalhamid, Nasreldin Elhadi, Samar Albunayan, Khaldoon Alsamman, Reem Aljindan
PURPOSE: The resistance determinants for carbapenems, fluoroquinolones and aminoglycosides were characterized in 16 extensively drug-resistant Klebsiella pneumoniae (XDRKPN) strains collected from Saudi hospitals during 2014. METHODOLOGY: PCR and sequencing were used to detect: blaKPC, blaNDM, blaVIM, blaIMP-1,blaOXA-48, blaCTX-M, blaTEM, blaSHV and ampC for β-lactam resistance; qnrA, qnrB, qnrS, aac(6')-Ib-cr, qepA and mutations of gyrA and parC for fluoroquinolone resistance; and aacA4, aacC2, aadA1, aphA6, armA and rmtB for aminoglycoside resistance...
July 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28618522/role-of-length-polydispersity-in-the-phase-behavior-of-freely-rotating-hard-rectangle-fluids
#17
Ariel Díaz-De Armas, Yuri Martínez-Ratón
We use the density-functional formalism, in particular the scaled-particle theory, applied to a length-polydisperse hard-rectangle fluid to study its phase behavior as a function of the mean particle aspect ratio κ_{0} and polydispersity Δ_{0}. The numerical solutions of the coexistence equations are calculated by transforming the original problem with infinite degrees of freedoms to a finite set of equations for the amplitudes of the Fourier expansion of the moments of the density profiles. We divide the study into two parts...
May 2017: Physical Review. E
https://www.readbyqxmd.com/read/28614492/clinical-improvement-in-patients-with-borderline-personality-disorder-after-treatment-with-repetitive-transcranial-magnetic-stimulation-preliminary-results
#18
Julian Reyes-López, Josefina Ricardo-Garcell, Gabriela Armas-Castañeda, María García-Anaya, Iván Arango-De Montis, Jorge J González-Olvera, Francisco Pellicer
Objective: Current treatment of borderline personality disorder (BPD) consists of psychotherapy and pharmacological interventions. However, the use of repetitive transcranial magnetic stimulation (rTMS) could be beneficial to improve some BPD symptoms. The objective of this study was to evaluate clinical improvement in patients with BPD after application of rTMS over the right or left dorsolateral prefrontal cortex (DLPFC). Method: Twenty-nine patients with BPD from the National Institute of Psychiatry, Mexico, were randomized in two groups to receive 15 sessions of rTMS applied over the right (1 Hz, n=15) or left (5 Hz, n=14) DLPFC...
June 12, 2017: Revista Brasileira de Psiquiatria
https://www.readbyqxmd.com/read/28605183/directed-self-assembly-of-colloidal-particles-onto-nematic-liquid-crystalline-defects-engineered-by-chemically-patterned-surfaces
#19
Xiao Li, Julio C Armas-Pérez, Juan P Hernández-Ortiz, Christopher G Arges, Xiaoying Liu, José A Martínez-González, Leonidas E Ocola, Camille Bishop, Helou Xie, Juan J de Pablo, Paul F Nealey
In exploiting topological defects of liquid crystals as the targeting sites for trapping colloidal objects, previous work has relied on topographic features with uniform anchoring to create defects, achieving limited density and spacing of particles. We report a generalizable strategy to create topological defects on chemically patterned surfaces to assemble particles in precisely defined locations with a tunable interparticle distance at nanoscale dimensions. Informed by experimental observations and numerical simulations that indicate that liquid crystals, confined between a homeotropic-anchoring surface and a surface with lithographically defined planar-anchoring stripes in a homeotropic-anchoring background, display splay-bend deformation, we successfully create pairs of defects and subsequently trap particles with controlled spacing by designing patterns of intersecting stripes aligned at 45° with homeotropic-anchoring gaps at the intersections...
June 27, 2017: ACS Nano
https://www.readbyqxmd.com/read/28584896/importance-of-tissue-sampling-laboratory-methods-and-patient-characteristics-for-detection-of-pneumocystis-in-autopsied-lungs-of-non-immunosuppressed-individuals
#20
REVIEW
S L Vargas, C Ponce, R Bustamante, E Calderón, G Nevez, Y De Armas, O Matos, R F Miller, M J Gallo
To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals.
June 5, 2017: European Journal of Clinical Microbiology & Infectious Diseases
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