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https://www.readbyqxmd.com/read/29204451/targeting-of-immune-cells-by-dual-tlr2-7-ligands-suppresses-features-of-allergic-th2-immune-responses-in-mice
#1
Jonathan Laiño, Andrea Wangorsch, Frank Blanco, Sonja Wolfheimer, Maren Krause, Adam Flaczyk, Tobias-Maximilian Möller, Mindy Tsai, Stephen Galli, Stefan Vieths, Masako Toda, Stephan Scheurer, Stefan Schülke
Background: TLR ligands can promote Th1-biased immune responses, mimicking potent stimuli of viruses and bacteria. Aim: To investigate the adjuvant properties of dual TLR2/7 ligands compared to those of the mixture of both single ligands. Methods: Dual TLR2/7 ligands: CL401, CL413, and CL531, including CL264 (TLR7-ligand) and Pam2CysK4 (TLR2-ligand), were used. Immune-modulatory capacity of the dual ligands with the individual ligands alone or as a mixture in mouse BMmDCs, BMmDC:TC cocultures, or BMCMCs was compared and assessed in naïve mice and in a mouse model of OVA-induced intestinal allergy...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/29159584/correction-to-optimized-synthesis-of-novel-prenyl-ferulate-performed-by-feruloyl-esterases-from-myceliophthora-thermophila-in-microemulsions
#2
Io Antonopoulou, Laura Leonov, Peter Jütten, Gabriella Cerullo, Vincenza Faraco, Adamantia Papadopoulou, Dimitris Kletsas, Marianna Ralli, Ulrika Rova, Paul Christakopoulos
After publication of the original article, authors found that there has been a minor mistake in the units of kcat and kcat/Km in Table 2. The units should be 10(3) min(-1) g(-1) FAE for kcat and mM(-1) min(-1) g(-1) FAE for kcat/Km. This correction does not affect any conclusions drawn within the article.
November 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29157672/kr%C3%A3-ppel-like-factors-family-expression-in-cervical-cancer-cells
#3
Daniel Marrero-Rodríguez, Hugo Arreola-De la Cruz, Keiko Taniguchi-Ponciano, Laura Gomez-Virgilio, Victor Huerta-Padilla, Gustavo Ponce-Navarrete, Sergio Andonegui-Elguera, Florinda Jimenez-Vega, Pablo Romero-Morelos, Miriam Rodriguez-Esquivel, Marco Meraz-Rios, Ma Del Pilar Figueroa-Corona, Alberto Monroy, Oscar Pérez-González, Mauricio Salcedo
BACKGROUND AND AIMS: Krüppel Like Factors (KLF) refers to a family of seventeen members of transcription factors. Involved in several cellular processes. As other cancer types, Cervical Cancer (CC) presents molecular deregulations in transcription factors, but especially Human Papilloma Virus (HPV) sequences. Here in this work we analyzed the mRNA expression of all KLF family members in CC-derived cell lines and CC tissues. METHODS: The cell lines used were HeLa, INBL, RoVa, C4-I, Ms751, ViPa, CaLo, SiHa, CaSki, C33a and ViBo and the non-tumorigenic HaCaT...
May 2017: Archives of Medical Research
https://www.readbyqxmd.com/read/29103742/percutaneous-endoscopic-gastrostomy-feeding-of-locally-advanced-oro-pharygo-laryngeal-cancer-patients-blenderized-or-commercial-food
#4
Pyrros Papakostas, Georgia Tsaousi, George Stavrou, Dimitrios Rachovitsas, Gavriil Tsiropoulos, Constantina Rova, Ioannis Konstantinidis, Antonios Michalopoulos, Vasilios Grosomanidis, Katerina Kotzampassi
OBJECTIVES: Head and neck cancer patients commonly suffer from severe malnutrition at the time of tentative diagnosis. Percutaneous Endoscopic Gastrostomy [PEG] feeding is now considered as an efficient tool to reduce nutritional deterioration alongside concurrent treatment. We undertook the challenge to retrospectively evaluate the impact of a commercial, disease-specific, feeding formula [Supportan, Fresenius Kabi, Hellas] versus blenderized family food on nutritional outcome. MATERIALS AND METHODS: This is a retrospective analysis of prospectively collected nutritional and anthropometric data at the time of PEG placement, at the 8th week [after treatment termination] and at 8 months [6mo of recovery from treatment]...
November 2017: Oral Oncology
https://www.readbyqxmd.com/read/28994785/aerosol-assisted-chemical-vapor-deposition-of-metal-oxide-structures-zinc-oxide-rods
#5
Stella Vallejos, Nade Z da Pizu Rova, Jan Čechal, Isabel Gràcia, Carles Cané
Whilst columnar zinc oxide (ZnO) structures in the form of rods or wires have been synthesized previously by different liquid- or vapor-phase routes, their high cost production and/or incompatibility with microfabrication technologies, due to the use of pre-deposited catalyst-seeds and/or high processing temperatures exceeding 900 °C, represent a drawback for a widespread use of these methods. Here, however, we report the synthesis of ZnO rods via a non-catalyzed vapor-solid mechanism enabled by using an aerosol-assisted chemical vapor deposition (CVD) method at 400 °C with zinc chloride (ZnCl2) as the precursor and ethanol as the carrier solvent...
September 14, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28974444/expression-purification-and-functional-analysis-of-an-antigen-targeting-fusion-protein-composed-of-cd40-ligand-and-the-c-terminal-fragment-of-ovalbumin
#6
Yunnuo Shi, Scott A Halperin, Song F Lee
Delivering antigen via molecules specifically targeting receptors on the surface of antigen-presenting cells is a strategy to improve immune responses. In this study, an antigen-targeting fusion protein (OVA-CD40LS) composed of the C-terminal fragment of ovalbumin and the extracellular domain of mouse CD40 ligand was constructed by genetic fusion. The OVA-CD40LS and the control OVA (rOVA) genes were cloned in Escherichia coli and over-expressed as insoluble proteins. The rOVA protein was purified from the insoluble fraction of E...
September 30, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28950987/cervical-cancer-cells-suppress-effector-functions-of-cytotoxic-t-cells-through-the-adenosinergic-pathway
#7
M L Mora-García, L R Ávila-Ibarra, R García-Rocha, B Weiss-Steider, J Hernández-Montes, C A Don-López, V Gutiérrez-Serrano, I J Titla-Vilchis, M C Fuentes-Castañeda, A Monroy-Mora, L F Jave-Suárez, R Chacón-Salinas, L Vallejo-Castillo, S M Pérez-Tapia, A Monroy-García
BACKGROUND: The expression of CD73 in tumor cells plays a significant role in the production of adenosine (Ado) that suppresses antitumor effector cells. METHODS: In this study we analyzed the capability of HPV-positive (HPV+) cervical cancer (CeCa) cell lines CaSki, SiHa, HeLa, and RoVa; and HPV-negative (HPV-) cell lines C33A and ViBo to produce Ado and inhibit effector functions of CD8+ T cells. RESULTS: HPV+ CeCa cells expressed significantly higher levels of CD73 in the membrane (p<0...
October 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28869123/sequential-high-gravity-ethanol-fermentation-and-anaerobic-digestion-of-steam-explosion-and-organosolv-pretreated-corn-stover
#8
Constantinos Katsimpouras, Maria Zacharopoulou, Leonidas Matsakas, Ulrika Rova, Paul Christakopoulos, Evangelos Topakas
The present work investigates the suitability of pretreated corn stover (CS) to serve as feedstock for high gravity (HG) ethanol production at solids-content of 24wt%. Steam explosion, with and without the addition of H2SO4, and organosolv pretreated CS samples underwent a liquefaction/saccharification step followed by simultaneous saccharification and fermentation (SSF). Maximum ethanol concentration of ca. 76g/L (78.3% ethanol yield) was obtained from steam exploded CS (SECS) with 0.2% H2SO4. Organosolv pretreated CS (OCS) also resulted in high ethanol concentration of ca...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28638714/swallowing-safety-in-parkinson-s-disease-after-zona-incerta-deep-brain-stimulation
#9
Stina Sundstedt, Lina Holmén, Elin Rova, Jan Linder, Erik Nordh, Katarina Olofsson
OBJECTIVES: The objective of this study was to examine swallowing function in patients with Parkinson's disease before and after caudal zona incerta deep brain (cZI DBS) surgery. The aims were to examine the effect of cZI DBS on swallowing safety regarding liquid and solid food, as well as to identify the effect of cZI DBS on body mass index (BMI) and specific items from part II of the Unified Parkinson's Disease Rating Scale (UPDRS). MATERIALS AND METHODS: The median age of the 14 patients was 57 years (range 46-71), with a median disease duration of 6 years (range 2-13)...
June 2017: Brain and Behavior
https://www.readbyqxmd.com/read/28515785/recombinant-expression-of-thermostable-processive-mteg5-endoglucanase-and-its-synergism-with-mtlpmo-from-myceliophthora-thermophila-during-the-hydrolysis-of-lignocellulosic-substrates
#10
Anthi Karnaouri, Madhu Nair Muraleedharan, Maria Dimarogona, Evangelos Topakas, Ulrika Rova, Mats Sandgren, Paul Christakopoulos
BACKGROUND: Filamentous fungi are among the most powerful cellulolytic organisms in terrestrial ecosystems. To perform the degradation of lignocellulosic substrates, these microorganisms employ both hydrolytic and oxidative mechanisms that involve the secretion and synergism of a wide variety of enzymes. Interactions between these enzymes occur on the level of saccharification, i.e., the release of neutral and oxidized products, but sometimes also reflected in the substrate liquefaction...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28487384/noninvasive-interrogation-of-dll3-expression-in-metastatic-small-cell-lung-cancer
#11
Sai Kiran Sharma, Jacob Pourat, Dalya Abdel-Atti, Sean D Carlin, Alessandra Piersigilli, Alexander J Bankovich, Eric E Gardner, Omar Hamdy, Kumiko Isse, Sheila Bheddah, Joseph Sandoval, Kristen M Cunanan, Eric B Johansen, Viola Allaj, Vikram Sisodiya, David Liu, Brian M Zeglis, Charles M Rudin, Scott J Dylla, John T Poirier, Jason S Lewis
The Notch ligand DLL3 has emerged as a novel therapeutic target expressed in small cell lung cancer (SCLC) and high-grade neuroendocrine carcinomas. Rovalpituzumab teserine (Rova-T; SC16LD6.5) is a first-in-class DLL3-targeted antibody-drug conjugate with encouraging initial safety and efficacy profiles in SCLC in the clinic. Here we demonstrate that tumor expression of DLL3, although orders of magnitude lower in surface protein expression than typical oncology targets of immunoPET, can serve as an imaging biomarker for SCLC...
July 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/28453727/endothelial-glycocalyx-breakdown-is-mediated-by-angiopoietin-2
#12
Alexander Lukasz, Carina Hillgruber, Hans Oberleithner, Kristina Kusche-Vihrog, Hermann Pavenstädt, Alexandros Rovas, Bettina Hesse, Tobias Goerge, Philipp Kümpers
Aims: The endothelial glycocalyx (eGC), a carbohydrate-rich layer lining the luminal surface of the endothelium, provides a first vasoprotective barrier against vascular leakage and adhesion in sepsis and vessel inflammation. Angiopoietin-2 (Angpt-2), an antagonist of the endothelium-stabilizing receptor Tie2 secreted by endothelial cells, promotes vascular permeability through cellular contraction and junctional disintegration. We hypothesized that Angpt-2 might also mediate the breakdown of the eGC...
May 1, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28427428/quality-issues-with-malaria-rapid-diagnostic-test-accessories-and-buffer-packaging-findings-from-a-5-country-private-sector-project-in-africa
#13
Steven A Harvey, Sandra Incardona, Nina Martin, Cristina Lussiana, Elizabeth Streat, Stephanie Dolan, Nora Champouillon, Daniel J Kyabayinze, Robert Mugerwa, Grace Nakanwagi, Nancy Njoki, Ratsimandisa Rova, Jane Cunningham
BACKGROUND: Use of antigen-detecting malaria rapid diagnostic tests (RDTs) has increased exponentially over the last decade. WHO's Global Malaria Programme, FIND, and other collaborators have established a quality assurance scheme to guide product selection, lot verification, transport, storage, and training procedures. Recent concerns over the quality of buffer packaging and test accessories suggest a need to include these items in product assessments. This paper describes quality problems with buffer and accessories encountered in a project promoting private sector RDT use in five African countries and suggests steps to avoid or more rapidly identify and resolve such problems...
April 20, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28412146/acetate-detoxification-of-wood-hydrolysates-with-alkali-tolerant-bacillus-sp-as-a-strategy-to-enhance-the-lipid-production-from-rhodosporidium-toruloides
#14
Leonidas Matsakas, Katharina Novak, Josefine Enman, Paul Christakopoulos, Ulrika Rova
The aim of the current work was to convert an acetate-rich hemicellulose liquid fraction (LF) from hot-water extraction of Betula pendula to oils for biodiesel, with Rhodosporidium toruloides. The toxicity of acetate was circumvented by biological detoxification with an isolated alkali-tolerant and acetate-resistant Bacillus sp. strain. Removal of other lignocellulose-derived inhibitors, such as furfural and phenols, was evaluated by two strategies; an activated carbon (AC) treatment of the undiluted LF, and dilution of the LF by 25% (0...
October 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28382684/thromboembolism-and-in-vitro-fertilization-a-systematic-review
#15
REVIEW
Maria Sennström, Karin Rova, Margareta Hellgren, Ragnhild Hjertberg, Eva Nord, Lars Thurn, Pelle G Lindqvist
INTRODUCTION: There is no accepted consensus on thromboprophylaxis in relation to in vitro fertilization (IVF). We aimed to study the frequency of thromboembolism and to assess thromboprophylaxis in relation to IVF. MATERIAL AND METHODS: We performed a systematic review. All study designs were accepted except single case reports. Language of included articles was restricted to English. RESULTS: Of 338 articles, 21 relevant articles (nine cohort studies, six case-control studies, three case series, and three reviews of case series) were identified...
September 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28165115/endothelial-glycocalyx-breakdown-is-mediated-by-angiopoietin-2
#16
Alexander Lukasz, Carina Hillgruber, Hans Oberleithner, Kristina Kusche-Vihrog, Hermann Pavenstädt, Alexandros Rovas, Bettina Hesse, Tobias Goerge, Philipp Kümpers
No abstract text is available yet for this article.
February 6, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28078397/optimized-synthesis-of-novel-prenyl-ferulate-performed-by-feruloyl-esterases-from-myceliophthora-thermophila-in-microemulsions
#17
Io Antonopoulou, Laura Leonov, Peter Jütten, Gabriella Cerullo, Vincenza Faraco, Adamantia Papadopoulou, Dimitris Kletsas, Marianna Ralli, Ulrika Rova, Paul Christakopoulos
Five feruloyl esterases (FAEs; EC 3.1.1.73), FaeA1, FaeA2, FaeB1, and FaeB2 from Myceliophthora thermophila C1 and MtFae1a from M. thermophila ATCC 42464, were tested for their ability to catalyze the transesterification of vinyl ferulate (VFA) with prenol in detergentless microemulsions. Reaction conditions were optimized investigating parameters such as the medium composition, the substrate concentration, the enzyme load, the pH, the temperature, and agitation. FaeB2 offered the highest transesterification yield (71...
April 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28006011/a-precise-temperature-responsive-bistable-switch-controlling-yersinia-virulence
#18
Aaron Mischa Nuss, Franziska Schuster, Louisa Roselius, Johannes Klein, René Bücker, Katharina Herbst, Ann Kathrin Heroven, Fabio Pisano, Christoph Wittmann, Richard Münch, Johannes Müller, Dieter Jahn, Petra Dersch
Different biomolecules have been identified in bacterial pathogens that sense changes in temperature and trigger expression of virulence programs upon host entry. However, the dynamics and quantitative outcome of this response in individual cells of a population, and how this influences pathogenicity are unknown. Here, we address these questions using a thermosensing virulence regulator of an intestinal pathogen (RovA of Yersinia pseudotuberculosis) as a model. We reveal that this regulator is part of a novel thermoresponsive bistable switch, which leads to high- and low-invasive subpopulations within a narrow temperature range...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27882195/design-and-synthesis-of-tesirine-a-clinical-antibody-drug-conjugate-pyrrolobenzodiazepine-dimer-payload
#19
Arnaud C Tiberghien, Jean-Noel Levy, Luke A Masterson, Neki V Patel, Lauren R Adams, Simon Corbett, David G Williams, John A Hartley, Philip W Howard
Pyrrolobenzodiazepine dimers are an emerging class of warhead in the field of antibody-drug conjugates (ADCs). Tesirine (SG3249) was designed to combine potent antitumor activity with desirable physicochemical properties such as favorable hydrophobicity and improved conjugation characteristics. One of the reactive imines was capped with a cathepsin B-cleavable valine-alanine linker. A robust synthetic route was developed to allow the production of tesirine on clinical scale, employing a flexible, convergent strategy...
November 10, 2016: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27492011/plasmid-ppcp1-derived-srna-hmsa-promotes-biofilm-formation-of-yersinia-pestis
#20
Zizhong Liu, Xiaofang Gao, Hongduo Wang, Haihong Fang, Yanfeng Yan, Lei Liu, Rong Chen, Dongsheng Zhou, Ruifu Yang, Yanping Han
BACKGROUND: The ability of Yersinia pestis to form a biofilm is an important characteristic in flea transmission of this pathogen. Y. pestis laterally acquired two plasmids (pPCP1and pMT1) and the ability to form biofilms when it evolved from Yersinia pseudotuberculosis. Small regulatory RNAs (sRNAs) are thought to play a crucial role in the processes of biofilm formation and pathogenesis. RESULTS: A pPCP1-derived sRNA HmsA (also known as sR084) was found to contribute to the enhanced biofilm formation phenotype of Y...
August 4, 2016: BMC Microbiology
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