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Bushra Uzair, Farid Menaa, Barkat Ali Khan, Faryal Vali Mohammad, Viqar Uddin Ahmad, Ryad Djeribi, Bouzid Menaa
AIMS: Screening of seaweed-associated bacteria capable of producing antimicrobials. METHODS AND RESULTS: Fifteen microbial strains, associated to the brown seaweed Pelvetia canaliculata (Linnaeus) attached to the rocks of Sonmiani Beach (Karachi, Pakistan), were screened. Crude extract filtrates of CMG S2 strain grew on Zobell marine agar (ZMA) had the most remarkable antimicrobial activity. Based on its phenotypic aspects (e.g. Gram-positive, microccoid form), biochemical characteristics (e...
January 2018: Microbiological Research
Yu-Fan Liu, Chia-Wen Hsieh, Yao-Sheng Chang, Being-Sun Wung
BACKGROUND: Acetic acid is a predominant by-product of lignocellulosic biofuel process, which inhibits microbial biocatalysts. Development of bacterial strains that are tolerant to acetic acid is challenging due to poor understanding of the underlying molecular mechanisms. RESULTS: In this study, we generated and characterized two acetic acid-tolerant strains of Zymomonas mobilis using N-methyl-N'-nitro-N-nitrosoguanidine (NTG)-acetate adaptive breeding. Two mutants, ZMA-142 and ZMA-167, were obtained, showing a significant growth rate at a concentration of 244 mM sodium acetate, while the growth of Z...
August 1, 2017: BMC Biotechnology
Fei Ge, Xing Huang, Hongmei Hu, Yanling Zhang, Zhaoling Li, Chaoying Zou, Huanwei Peng, Lujiang Li, Shibin Gao, Guangtang Pan, Yaou Shen
The induction efficiency of maize embryonic callus is highly dependent on the genotype, and only a few lines possess a high capacity for callus formation. Although certain genes and pathways have been reported to contribute to the regulation of callus induction, to the best of our knowledge, the functions of the small interfering RNAs (siRNAs) involved in this process remain unknown. In this study, we identified 861 differentially expressed siRNAs and 576 target genes in the callus induction process. These target genes were classified into 3 clusters, and their functions involve controlling metalloexopeptidase activity, catalase activity, transcription regulation, and O-methyltransferase activity...
2017: PloS One
Jayaraman Aravind, Sharma Rinku, Banduni Pooja, Mittal Shikha, Shiriga Kaliyugam, Mallana Gowdra Mallikarjuna, Arun Kumar, Atmakuri Ramakrishna Rao, Thirunavukkarasu Nepolean
MicroRNA-mediated gene regulation plays a crucial role in controlling drought tolerance. In the present investigation, 13 drought-associated miRNA families consisting of 65 members and regulating 42 unique target mRNAs were identified from drought-associated microarray expression data in maize and were subjected to structural and functional characterization. The largest number of members (14) was found in the zma-miR166 and zma-miR395 families, with several targets. However, zma-miR160, zma-miR390, zma-miR393, and zma-miR2275 each showed a single target...
2017: Frontiers in Plant Science
Zhan Zhao, Yadong Xue, Huili Yang, Huimin Li, Gaoyang Sun, Xiaofeng Zhao, Dong Ding, Jihua Tang
Internode length is one of the decisive factors affecting plant height (PH) and ear height (EH), which are closely associated with the lodging resistance, biomass and grain yield of maize. miRNAs, currently recognized as important transcriptional/ post-transcriptional regulators, play an essential role in plant growth and development. However, their roles in developing internodes under maize ears remain unclear. To identify the roles of miRNAs and their targets in the development of internodes under maize ears, six miRNA and two degradome libraries were constructed using the 7th, 8th and 9th internodes of two inbred lines, 'Xun928' and 'Xun9058', which had significantly different internode lengths...
2016: PloS One
Shiqing Xu, Ei-Ichi Negishi
Carbometalation of alkenes with stereocontrol offers an important opportunity for asymmetric C-C bond formation. However, the scope of catalytic stereoselective carbometalation of alkenes had until recently been limited to electronically biased alkenes or those with the presence of directing groups or other auxiliary functionalities to overcome the challenge associated with regio- and stereoselectivity. Catalytic asymmetric carbometalation of unactivated alkenes on the other hand remained as a formidable challenge...
October 18, 2016: Accounts of Chemical Research
Bei Wang, Fenglei Qian, Bin Hou, Lan Zheng, Chen Wang
No abstract text is available yet for this article.
May 2016: Medicine and Science in Sports and Exercise
Stefanie Dukowic-Schulze, Anitha Sundararajan, Thiruvarangan Ramaraj, Shahryar Kianian, Wojciech P Pawlowski, Joann Mudge, Changbin Chen
Small RNAs (sRNA) add additional layers to the regulation of gene expression, with siRNAs directing gene silencing at the DNA level by RdDM (RNA-directed DNA methylation), and micro RNAs (miRNAs) directing post-transcriptional regulation of specific target genes, mostly by mRNA cleavage. We used manually isolated male meiocytes from maize (Zea mays) to investigate sRNA and DNA methylation landscapes during zygotene, an early stage of meiosis during which steps of meiotic recombination and synapsis of paired homologous chromosomes take place...
2016: Frontiers in Plant Science
Mingming Xin, Guanghui Yang, Yingyin Yao, Huiru Peng, Zhaorong Hu, Qixin Sun, Xiangfeng Wang, Zhongfu Ni
In angiosperms, the endosperm nurtures the embryo and provides nutrients for seed germination. To identify the expression pattern of small interfering RNA in the developing maize endosperm, we have performed high-throughput small RNA transcriptome sequencing of kernels at 0, 3, and 5 days after pollination (DAP) and endosperms at 7, 10, and 15 DAP using B73 and Mo17 reciprocal crosses in previous study. Here, we further explored these small RNA-seq data to investigate the potential roles of miRNAs in regulating the gene expression process...
2015: Frontiers in Plant Science
Lei Sheng, Wenbo Chai, Xuefeng Gong, Lingyan Zhou, Ronghao Cai, Xiaoyu Li, Yang Zhao, Haiyang Jiang, Beijiu Cheng
MicroRNAs (miRNAs) are a class of small, non-coding regulatory RNAs that regulate gene expression by guiding target mRNA cleavage or translational inhibition in plants and animals. At present there is relatively little information regarding the role of miRNAs in the response to drought stress in maize. In this study, two small RNA libraries were sequenced, and a total of 11,973,711 and 14,326,010 raw sequences were generated from growing leaves of drought-tolerant and drought-sensitive maize seedlings, respectively...
2015: International Journal of Biological Sciences
James Sullivan, Nathan Cooze, Callum Gallagher, Tom Lewis, Tomas Prosek, Dominique Thierry
In situ time-lapse optical microscopy was used to examine the microstructural corrosion mechanisms in three zinc-magnesium-aluminium (ZMA) alloy coated steels immersed in 1% NaCl pH 7. Preferential corrosion of MgZn(2) lamellae within the eutectic phases was observed in all the ZMA alloys followed by subsequent dissolution of Zn rich phases. The total extent and rate of corrosion, measured using time-lapse image analysis and scanning vibrating electrode technique (SVET) estimated mass loss, decreased as Mg and Al alloying additions were increased up to a level of 3 wt% Mg and 3...
2015: Faraday Discussions
Mingda Luan, Miaoyun Xu, Yunming Lu, Lan Zhang, Yunliu Fan, Lei Wang
The miR169 miRNA family is highly conserved in plants. Its members regulate the expression of genes encoding the universal transcription factor subunit NUCLEAR FACTOR-Y subunit A (NF-YA) via transcript cleavage. NF-YA regulates gene expression by binding the CCAAT box sequence in target promoters. The miR169/NF-YA module plays a critical role during plant development and in plant responses to abiotic stress. We characterized the secondary structures of maize pre-miR169 miRNAs and predicted their potential gene targets...
January 25, 2015: Gene
Antonio Bonfitto, Mauro Morassi
The genus Horaiclavus includes eight Holocene Indo-Pacific species (Appeltans et al. 2012). Herein, we describe two new species that resemble members of this genus in some aspects of shell morphology, but otherwise show features that suggest that they differ from "typical" Horaiclavus species. Descriptions and measurements were based on shells oriented spire up with the aperture facing the viewer and made using a Leica MS 5 Stereomicroscope for incident light and a 10× ocular micrometer. Scanning electron microscope (SEM) micrographs were taken using a Hitachi S-2400...
2014: Zootaxa
Anna Lukasik, Piotr Zielenkiewicz
MicroRNAs (miRNAs) are a class of small RNA molecules that regulate gene expression by inhibiting the protein translation or targeting the mRNA cleavage. They play many important roles in living organism cells; however, the knowledge on miRNAs functions has become more extensive upon their identification in biological fluids and recent reports on plant-origin miRNAs abundance in human plasma and serum. Considering these findings, we performed a rigorous bioinformatics analysis of publicly available, raw data from high-throughput sequencing studies on miRNAs composition in human and porcine breast milk exosomes to identify the fraction of food-derived miRNAs...
2014: PloS One
Mingda Luan, Miaoyun Xu, Yunming Lu, Qiuxue Zhang, Lan Zhang, Chunyi Zhang, Yunliu Fan, Zhihong Lang, Lei Wang
Previous studies have identified miR169/NF-YA modules are important regulators of plant development and stress responses. Currently, reported genome sequence data offers an opportunity for global characterization of miR169 and NF-YA genes, which may provide insights into the molecular mechanisms of the miR169/NF-YA modules in maize. In our study, fourteen NF-YA transcription factors with conserved domains were identified based on maize genome loci. The miR169 gene family has 18 members that generate 10 mature products, and 8 of these mature miR169 members could target 7 of 14 ZmNF-YA genes in maize...
2014: PloS One
Jun Wang, Jing-Shu Huang, Xin-Yan Hao, Yan-Ping Feng, Ya-Jun Cai, Li-Qin Sun
MicroRNAs (miRNAs) are 20-24 nucleotide long non-coding RNAs that play critical regulatory roles during plant development, organ morphogenesis, and cell fate determination and differentiation. In this study, miRNA microarray chips were used to explore the expression profile of ramie miRNAs between the bast of fiber elongation phase and those of cell wall thickening and end wall dissolving phase. There are 150 and 148 credible miRNAs in the bast of fiber elongation phase and cell wall thickening and end wall dissolving phase, respectively...
February 2014: Molecular Biology Reports
Götz Fabry, Martin R Fischer
No abstract text is available yet for this article.
2013: GMS Zeitschrift Für Medizinische Ausbildung
Dawn E Watkins-Chow, Joanna Cooke, Ruth Pidsley, Andrew Edwards, Rebecca Slotkin, Karen E Leeds, Raymond Mullen, Laura L Baxter, Thomas G Campbell, Marion C Salzer, Laura Biondini, Gretchen Gibney, Françoise Phan Dinh Tuy, Jamel Chelly, H Douglas Morris, Johannes Riegler, Mark F Lythgoe, Ruth M Arkell, Fabrizio Loreni, Jonathan Flint, William J Pavan, David A Keays
The ribosome is an evolutionarily conserved organelle essential for cellular function. Ribosome construction requires assembly of approximately 80 different ribosomal proteins (RPs) and four different species of rRNA. As RPs co-assemble into one multi-subunit complex, mutation of the genes that encode RPs might be expected to give rise to phenocopies, in which the same phenotype is associated with loss-of-function of each individual gene. However, a more complex picture is emerging in which, in addition to a group of shared phenotypes, diverse RP gene-specific phenotypes are observed...
2013: PLoS Genetics
Stefanie P Glaeser, A B Arun, P D Rekha, Sudharshan Prabhu, Hans-Jürgen Busse, Peter Kämpfer
A Gram-positive-staining, aerobic, non-endospore-forming bacterium, isolated from Ullal coastal sand, Mangalore, Karnataka, India, on marine agar 2216, was studied in detail for its taxonomic position. Based on 16S rRNA gene sequence similarity comparisons, strain ZMA 19(T) was grouped into the genus Bhargavaea with high 16S rRNA gene sequence similarities to all currently described species of the genus Bhargavaea, Bhargavaea cecembensis (99.3 %), Bhargavaea beijingensis (98.8 %) and Bhargavaea ginsengi (98...
July 2013: International Journal of Systematic and Evolutionary Microbiology
Kiriakos Daniilidis, Carsten O Tibesku
PURPOSE: Although total knee arthroplasty (TKA) is regularly associated with favorable outcomes, considerable research efforts are still underway to improve its ability to achieve a neutral postoperative mechanical axis. Patient-specific instrumentation (PSI) was introduced with this and other goals in mind. The current retrospective study was designed to determine whether PSI would lead to a hip-knee-ankle angle (HKA) within ±3° of the ideal alignment of 180°. METHODS: A long-leg x-ray of the knee was performed after an average of 3...
January 2013: International Orthopaedics
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