Vesna Spasovski, Anna Romolo, Urška Zagorc, Vesna Arrigler, Matic Kisovec, Apolonija Bedina Zavec, Matevž Arko, Adrienn Molnár, Gitta Schlosser, Aleš Iglič, Ksenija Kogej, Veronika Kralj-Iglič
INTRODUCTION: Lipid nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called nano-sized hybridosomes or nanohybridosomes, NSHs) were considered. METHODS: We formed NSHs by mixing appropriate amounts of lecithin, glycerol and supernatant of isolation of extracellular vesicles from spruce needle homogenate. We visualized NSHs by light microscopy and cryogenic transmission electron microscopy and assessed them by flow cytometry, dynamic light scattering, ultraviolet-visual spectroscopy, interferometric light microscopy and liquid chromatography-mass spectrometry...
2024: International Journal of Nanomedicine