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Keratinocyte activation

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https://www.readbyqxmd.com/read/28231625/experimental-study-on-1-25-oh-2-d3-amelioration-of-oral-lichen-planus-through-regulating-nf-%C3%AE%C2%BAb-signaling-pathway
#1
Jie Du, Ran Li, Feiyan Yu, Fang Yang, Jinhua Wang, Qingyong Chen, Xiangyu Wang, Bin Zhao, Fang Zhang
OBJECTIVE: To explore the protective function of vitamin D (VD)/vitamin D receptor (VDR) on the development of oral lichen planus (OLP) and elaborate the underling mechanism of it. METHODS: H&E staining, myeloid Peroxidase (MPO) assays, quantitative PCR (qPCR), western blotting and Elisa were used to test the human biopsies and serum. QPCR, western blotting, Elisa and siRNA transfection were also performed in LPS-induced keratinocytes to observe the functions of vitamin D and VDR...
February 23, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28230860/carma2sh-and-ulk2-control-pathogen-associated-molecular-patterns-recognition-in-human-keratinocytes-psoriasis-linked-carma2sh-mutants-escape-ulk2-censorship
#2
Ivan Scudiero, Pellegrino Mazzone, Luca E D'Andrea, Angela Ferravante, Tiziana Zotti, Gianluca Telesio, Gabriele De Rubis, Carla Reale, Maddalena Pizzulo, Shanmugakonar Muralitharan, Pasquale Vito, Romania Stilo
The molecular complexes formed by specific members of the family of CARMA proteins, the CARD domain-containing adapter molecule BCL10 and MALT1 (CBM complex) represent a central hub in regulating activation of the pleiotropic transcription factor NF-κB. Recently, missense mutations in CARMA2sh have been shown to cause psoriasis in a dominant manner and with high penetrancy. Here, we demonstrate that in human keratinocytes CARMA2sh plays an essential role in the signal transduction pathway that connects pathogen-associated molecular patterns recognition to NF-κB activation...
February 23, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28229920/in-vitro-wound-healing-and-cytotoxic-activity-of-the-gel-and-whole-leaf-materials-from-selected-aloe-species
#3
Lizelle T Fox, Anisha Mazumder, Anupma Dwivedi, Minja Gerber, Jeanetta du Plessis, Josias H Hamman
ETHNOPHARMACOLOGICAL RELEVANCE: Aloe vera is one of the most important medicinal plants in the world with applications in the cosmetic industry and also in the tonic or health drink product market. Different parts of Aloe ferox and Aloe marlothii are used as traditional medicines for different applications. Although wound healing has been shown for certain aloe gel materials (e.g. A. vera) previously, there are conflicting reports on this medicinal application of aloe leaf gel materials...
February 13, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28229230/mycobacterium-ulcerans-mycolactone-interferes-with-adhesion-migration-and-proliferation-of-primary-human-keratinocytes-and-hacat-cell-line
#4
Francesca Graziola, Elena Colombo, Rossana Tiberio, Giorgio Leigheb, Chiarella Bozzo
The pathogenicity of Mycobacterium ulcerans (Buruli ulcer) is closely associated with the secretion of exotoxin mycolactone. The cytotoxicity of mycolactone has been linked to its apoptogenic activity. We explored if low mycolactone concentrations, which are not able to induce apoptosis, can influence other essential activities on two primary human keratinocyte populations, keratinocyte stem cells (KSC) and transit amplifying cells (TAC), and on a human keratinocyte line, HaCaT. We demonstrated that 0.01 and 0...
February 22, 2017: Archives of Dermatological Research
https://www.readbyqxmd.com/read/28228560/expression-of-il-22-in-the-skin-causes-th2-biased-immunity-epidermal-barrier-dysfunction-and-pruritus-via-stimulating-epithelial-th2-cytokines-and-the-grp-pathway
#5
Hongfei Lou, Jingning Lu, Eun Byul Choi, Min Hee Oh, Mingeum Jeong, Sara Barmettler, Zhou Zhu, Tao Zheng
Increased expression of Th22 cytokine IL-22 is a characteristic finding in atopic dermatitis (AD). However, the specific role of IL-22 in the pathogenesis of AD in vivo has yet to be elucidated. Consistent with observations in human AD, IL-22 was significantly increased in the AD skin of mice after epicutaneous sensitization to house dust mite allergen. Utilizing a skin-specific inducible transgenic system, we show in the present study that expression of IL-22 in the skin of mice caused an AD-like phenotype characterized by chronic pruritic dermatitis associated with Th2-biased local and systemic immune responses, downregulation of epidermal differentiation complex genes, and enhanced dermatitis upon epicutaneous allergen exposure...
February 22, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28225180/microrna-216b-inhibits-cell-proliferation-and-migration-in-human-melanoma-by-targeting-foxm1-in-vitro-and-in-vivo
#6
Mengyao Sun, Xiaopeng Wang, Chen Tu, Shuang Wang, Jianqiang Qu, Shengxiang Xiao
MicroRNAs (miRNAs) play an increasingly important role in cancer growth by coordinately suppressing genes that controlcell migration, proliferationand invasion. The above results can be achieved through the regulation of gene expression by miRNAs bysuppressing translation or the directsequence-specific degradation of the targetedmRNA. In the present study, we indicate that the expression of miR-216b could be effectively repressed both in human melanoma tissues through a comparison with primary melanoma and in human melanoma cell lines through a comparison with a normal human keratinocyte line...
February 22, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28222435/extracorporal-shock-waves-activate-migration-proliferation-and-inflammatory-pathways-in-fibroblasts-and-keratinocytes-and-improve-wound-healing-in-an-open-label-single-arm-study-in-patients-with-therapy-refractory-chronic-leg-ulcers
#7
Ilknur Aschermann, Seema Noor, Sascha Venturelli, Tobias Sinnberg, Christian Busch, Christian D Mnich
BACKGROUND/AIMS: Chronic leg ulcers (CLUs) are globally a major cause of morbidity and mortality with increasing prevalence. Their treatment is highly challenging, and many conservative, surgical or advanced therapies have been suggested, but with little overall efficacy. Since the 1980s extracorporal shock wave therapy (ESWT) has gained interest as treatment for specific indications. Here, we report that patients with CLU showed wound healing after ESWT and investigated the underlying molecular mechanisms...
February 20, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28222363/very-short-term-reactive-forecasting-of-the-solar-ultraviolet-index-using-an-extreme-learning-machine-integrated-with-the-solar-zenith-angle
#8
Ravinesh C Deo, Nathan Downs, Alfio V Parisi, Jan F Adamowski, John M Quilty
Exposure to erythemally-effective solar ultraviolet radiation (UVR) that contributes to malignant keratinocyte cancers and associated health-risk is best mitigated through innovative decision-support systems, with global solar UV index (UVI) forecast necessary to inform real-time sun-protection behaviour recommendations. It follows that the UVI forecasting models are useful tools for such decision-making. In this study, a model for computationally-efficient data-driven forecasting of diffuse and global very short-term reactive (VSTR) (10-min lead-time) UVI, enhanced by drawing on the solar zenith angle (θs) data, was developed using an extreme learning machine (ELM) algorithm...
February 18, 2017: Environmental Research
https://www.readbyqxmd.com/read/28220862/induction-of-hair-follicle-dermal-papilla-cell-properties-in-human-induced-pluripotent-stem-cell-derived-multipotent-lngfr-thy-1-mesenchymal-cells
#9
Ophelia Veraitch, Yo Mabuchi, Yumi Matsuzaki, Takashi Sasaki, Hironobu Okuno, Aki Tsukashima, Masayuki Amagai, Hideyuki Okano, Manabu Ohyama
The dermal papilla (DP) is a specialised mesenchymal component of the hair follicle (HF) that plays key roles in HF morphogenesis and regeneration. Current technical difficulties in preparing trichogenic human DP cells could be overcome by the use of highly proliferative and plastic human induced pluripotent stem cells (hiPSCs). In this study, hiPSCs were differentiated into induced mesenchymal cells (iMCs) with a bone marrow stromal cell phenotype. A highly proliferative and plastic LNGFR(+)THY-1(+) subset of iMCs was subsequently programmed using retinoic acid and DP cell activating culture medium to acquire DP properties...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220834/stellera-chamaejasme-and-its-constituents-induce-cutaneous-wound-healing-and-anti-inflammatory-activities
#10
Myungsuk Kim, Hee Ju Lee, Ahmad Randy, Ji Ho Yun, Sang-Rok Oh, Chu Won Nho
Stellera chamaejasme L. (Thymelaeaceae) is a perennial herb that is widely used in traditional Chinese medicine to treat tumours, tuberculosis and psoriasis. S. chamaejasme extract (SCE) possesses anti-inflammatory, analgesic and wound healing activities; however, the effect of S. chamaejasme and its active compounds on cutaneous wound healing has not been investigated. We assessed full-thickness wounds of Sprague-Dawley (SD) rats and topically applied SCE for 2 weeks. In vitro studies were performed using HaCaT keratinocytes, Hs68 dermal fibroblasts and RAW 264...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28218450/chromium-iv-exposure-via-src-ras-signaling-promotes-cell-transformation
#11
Suthakar Ganapathy, Ping Li, Jean Lafontant, Rui Xiong, Tianqi Yu, Guojun Zhang, Changyan Chen
Hexavalent chromium [Cr(VI)] is a well-known environment carcinogen. The exposure of Cr(VI) through contaminated soil, air particles and drinking water is a strong concern for the public health worldwide. While many studies have been done, it remains unclear which intracellular molecules transduce Cr(VI)-mediated carcinogenic signaling in cells to promote cancer. In this study, we demonstrated that upon Cr(VI) treatment, the intracellular receptor src was activated, which further upregulated Ras activity, leading to the augmentation of ROS and onset of ER stress in human lung epithelial BEAS-2B or keratinocytes...
February 20, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28218425/the-role-of-lipid-raft-translocation-of-prohibitin-in-regulation-of-akt-and-raf-protected-apoptosis-of-hacat-cells-upon-ultraviolet-b-irradiation
#12
Qiong Wu, Shiyong Wu
Prohibitin (PHB) plays a role in regulation of ultraviolet B light (UVB)-induced apoptosis of human keratinocytes, HaCaT cells. The regulatory function of PHB appears to be associated with its lipid raft translocation. However, the detailed mechanism for PHB-mediated apoptosis of these keratinocytes upon UVB irradiation is not clear. In this report, we determined the role of lipid raft translocation of PHB in regulation of UVB-induced apoptosis. Our data show that upon UVB irradiation PHB is translocated from the non-raft membrane to the lipid rafts, which is correlated with a release of both Akt and Raf from membrane...
February 20, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28216888/effect-of-absolute-from-hibiscus-syriacus-l-flower-on-wound-healing-in-keratinocytes
#13
Seok Won Yoon, Kang Pa Lee, Do-Yoon Kim, Dae Il Hwang, Kyung-Jong Won, Dae Won Lee, Hwan Myung Lee
BACKGROUND: Proliferation and migration of keratinocytes are essential for the repair of cutaneous wounds. Hibiscus syriacus L. has been used in Asian medicine; however, research on keratinocytes is inadequate. OBJECTIVE: To establish the dermatological properties of absolute from Hibiscus syriacus L. flower (HSF) and to provide fundamental research for alternative medicine. MATERIALS AND METHODS: We identified the composition of HSF absolute using gas chromatography-mass spectrometry analysis...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28216604/evaluation-of-not-activated-and-activated-prp-in-hair-loss-treatment-role-of-growth-factor-and-cytokine-concentrations-obtained-by-different-collection-systems
#14
Pietro Gentile, John P Cole, Megan A Cole, Simone Garcovich, Alessandra Bielli, Maria Giovanna Scioli, Augusto Orlandi, Chiara Insalaco, Valerio Cervelli
Platelet rich plasma (PRP) was tested as a potential therapy for androgenetic alopecia (AGA) through two different clinical protocols in which one population (18 participants) received half-head treatment with autologous non-activated PRP (A-PRP) produced by CPunT Preparation System (Biomed Device, Modena, Italy) and the other half-head with placebo, and a second separated population in which all participants (n = 6, 3 participants per group) received treatment with calcium-activated PRP (AA-PRP) produced from one of two different PRP collection devices (Regen Blood Cell Therapy or Arthrex Angel System)...
February 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28214842/inhibition-of-methylglyoxal-induced-ages-rage-expression-contributes-to-dermal-protection-by-n-acetyl-l-cysteine
#15
Chun-Tao Yang, Fu-Hui Meng, Li Chen, Xiang Li, Lai-Jian Cen, Yu-Hua Wen, Cai-Chen Li, Hui Zhang
BACKGROUND/AIM: Accumulation of advanced glycation end products (AGEs) is a major cause of diabetes mellitus (DM) skin complications. Methylglyoxal (MGO), a reactive dicarbonyl compound, is a crucial intermediate of AGEs generation. N-acetyl-L-cysteine (NAC), an active ingredient of some medicines, can induce endogenous GSH and hydrogen sulfide generation, and set off a condensation reaction with MGO. However, there is rare evidence to show NAC can alleviate DM-induced skin injury through inhibition of AGEs generation or toxicity...
13, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28211872/rictor-mtorc2-deficiency-enhances-keratinocyte-stress-tolerance-via-mitohormesis
#16
Beatrice Tassone, Stefania Saoncella, Francesco Neri, Ugo Ala, Davide Brusa, Mark A Magnuson, Paolo Provero, Salvatore Oliviero, Chiara Riganti, Enzo Calautti
How metabolic pathways required for epidermal tissue growth and remodeling influence the ability of keratinocytes to survive stressful conditions is still largely unknown. The mechanistic target of rapamycin complex 2 (mTORC2) regulates growth and metabolism of several tissues, but its functions in epidermal cells are poorly defined. Rictor is an adaptor protein essential for mTORC2 activity. To explore the roles of mTORC2 in the epidermis, we have conditionally deleted rictor in mice via K14-Cre-mediated homologous recombination and found that its deficiency causes moderate tissue hypoplasia, reduced keratinocyte proliferation and attenuated hyperplastic response to TPA...
February 17, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28210712/identification-of-an-epidermal-keratinocyte-ampa-glutamate-receptor-involved-in-dermatopathies-associated-with-sensory-abnormalities
#17
David Cabañero, Takeshi Irie, Marta Celorrio, Christopher Trousdale, David M Owens, David Virley, Phillip J Albrecht, Michael J Caterina, Frank L Rice, Jose A Morón
INTRODUCTION: Epidermal keratinocytes are increasingly recognized as active participants in the sensory transduction of itch and pain, processes known to involve primary afferent glutamatergic neurons. However the role of keratinocyte glutamate signaling in sensory functioning is not fully understood. Here, we present the observation of α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid type glutamate receptors (AMPAR) in epidermal keratinocytes. METHODS: Immunohistochemical and in situ hybridization analyses were conducted to assess the expression of AMPAR subunits in epidermal keratinocytes in mouse and human skin samples, and in organotypic cultures of human keratinocytes...
September 2016: Pain Reports (Baltimore, Md.)
https://www.readbyqxmd.com/read/28210627/hyperforin-hp-%C3%AE-cyclodextrin-enhances-mechanosensitive-ca-2-signaling-in-hacat-keratinocytes-and-in-atopic-skin-ex-vivo-which-accelerates-wound-healing
#18
Hiroya Takada, Jun Yonekawa, Masami Matsumoto, Kishio Furuya, Masahiro Sokabe
Cutaneous wound healing is accelerated by mechanical stretching, and treatment with hyperforin, a major component of a traditional herbal medicine and a known TRPC6 activator, further enhances the acceleration. We recently revealed that this was due to the enhancement of ATP-Ca(2+) signaling in keratinocytes by hyperforin treatment. However, the low aqueous solubility and easy photodegradation impede the topical application of hyperforin for therapeutic purposes. We designed a compound hydroxypropyl-β-cyclodextrin- (HP-β-CD-) tetracapped hyperforin, which had increased aqueous solubility and improved photoprotection...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28209602/a-novel-murine-model-of-esophageal-non-erosive-reflux-disease-nerd-from-inflammation-to-impairment-in-mucosal-integrity
#19
Renan O Silva, Francisco Fábio B Oliveira, Rudy D Bingana, Mailton O Arruda, Philip Woodland, Chung Lee, Miguel A Nobre E Souza, Pedro Marcos G Soares, Armenio A Santos, Daniel Sifrim, Marcellus H L P Souza
Non-erosive reflux disease (NERD) is a highly prevalent phenotype of the gastroesophageal reflux disease (GERD). In this study, we developed a novel murine model of NERD in mice with microscopic inflammation and impairment in the epithelial esophageal barrier. Female Swiss mice were subjected to the following surgical procedure: the transitional region between the forestomach and the glandular portion of the stomach was ligated, and a nontoxic ring was placed around the duodenum near the pylorus. The control group underwent sham surgery...
February 16, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/28208984/aquaporin-3-and-e-cadherin-expression-in-perilesional-vitiligo-skin
#20
Ola Ahmed Bakry, Magda Mostafa Hagag, Mona Abd El Halim Kandil, Wafaa Ahmed Shehata
INTRODUCTION: Vitiligo is a common dermatologic disorder with debated aetiology. Most studies focused on role of melanocytes and few investigated the role of keratinocytes in pathogenesis of the disease. AIM: To investigate the keratinocyte adhesion in perilesional vitiligo skin through the immunolocalization of Aquaporin-3 (AQP3) and E-cadherin. SETTING AND DESIGN: Sixty five subjects were selected. These included 40 cases with vitiligo and 25 age and gender-matched healthy subjects as a control group...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
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