keyword
MENU ▼
Read by QxMD icon Read
search

Keratinocyte activation

keyword
https://www.readbyqxmd.com/read/29471244/two-sesquiterpene-aminoquinones-protect-against-oxidative-injury-in-hacat-keratinocytes-via-activation-of-ampk%C3%AE-erk-nrf2-are-ho-1-signaling
#1
Li Liu, Wei Wu, Jing Li, Wei-Hua Jiao, Li-Yun Liu, Jie Tang, Lei Liu, Fan Sun, Bing-Nan Han, Hou-Wen Lin
AIMS: To investigate the cytoprotective effects of two sesquiterpene aminoquinones isolated from the marine sponge Dysidea fragilis, Dysidaminone H (DA8) and 3'-methylamino-avarone (DA14), we examined their effects against hydrogen peroxide (H2 O2 )-induced oxidative injury in human keratinocyte cell line and elucidated the underlying mechanisms. MAIN METHODS: Cell viability was detected using a CCK-8 assay kit. Intracellular reactive oxygen species (ROS) production was measured by fluorescence of 2, 7-Dichlorodi-hydrofluorescein diacetate (DCFH-DA)...
February 19, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29470442/phycocyanin-protects-against-uvb-induced-apoptosis-through-the-pkc-%C3%AE-%C3%AE-ii-nrf-2-ho-1-dependent-pathway-in-human-primary-skin-cells
#2
Ki Mo Kim, Joo Young Lee, A-Rang Im, Sungwook Chae
Phycocyanin (Pc) is one of the active pigment constituents of Spirulina microalgae. It has been used for its potent antioxidant and anti-inflammatory properties. However, the protective effects of Pc against ultraviolet-B (UVB)-induced primary skin cells damage are still undefined. In the present study, we investigated whether Pc prevented UVB-induced apoptotic cell death in human dermal fibroblasts (HDF) and human epidermal keratinocytes (HEK). Pc induced the transcription of heme oxygenase-1 (HO-1). Furthermore, Pc treatments resulted in a marked increase in nuclear factor erythroid-derived 2 (NF-E2)-like 2 (Nrf-2) nuclear translocation...
February 22, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29468680/proinflammatory-role-of-blister-fluid-derived-exosomes-in-bullous-pemphigoid
#3
Hui Fang, Shuai Shao, Man Jiang, Erle Dang, Shengxian Shen, Jieyu Zhang, Pei Qiao, Caixia Li, Gang Wang
Bullous pemphigoid is an autoimmune inflammatory disorder characterised by the presence of autoantibodies against bullous pemphigoid autoantigens, leading to dermal-epidermal separation with consequent blister formation. However, whether and how the components of blister fluid exacerbate the progression of bullous pemphigoid is unclear. Exosomes are nanometre-sized vesicles released from cells into the body fluid, where they can transmit signals throughout the body. In the present study, we isolated and characterised exosomes from the blister fluids of patients with bullous pemphigoid, evaluated their proinflammatory role, and identified the underlying molecular mechanisms...
February 22, 2018: Journal of Pathology
https://www.readbyqxmd.com/read/29467931/toll-like-receptor-2-induces-adenosine-receptor-a2a-and-promotes-human-squamous-carcinoma-cell-growth-via-extracellular-signal-regulated-kinases-%C3%A2
#4
Chithra Devi Palani, Lalitha Ramanathapuram, Aroonwan Lam-Ubol, Zoya B Kurago
Patient treatment for oral squamous cell carcinoma (OSCC) not associated with Human papillomavirus remains problematic. OSCC microenvironment is typically inflamed and colonized by microorganisms, providing ligands for toll-like receptors (TLR). In immune cells TLR2 and TLR4 activate NF-kB and extracellular signal regulated kinase (ERK)1/2 pathways, leading to upregulation of inhibitory adenosine receptors A2a and A2b, and reduction in stimulatory A1 and A3. How TLR and adenosine receptors function in SCC cells is not understood...
January 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29467909/apoptosis-sensitizing-activity-of-birinapant-in-head-and-neck-squamous-cell-carcinoma-cell-lines
#5
Roman C Brands, Mario J J Scheurer, Stefan Hartmann, Axel Seher, Alexander C Kübler, Urs D A Müller-Richter
Inhibitor of apoptosis proteins, which are overexpressed in head and neck squamous cell carcinoma (HNSCC), may cause therapeutic resistance. Using SMAC mimetic compounds, including birinapant, to degrade and/or inhibit these proteins and sensitize apoptosis may enhance therapies in HNSCC. Fas expression was analyzed in nine HNSCC cell lines and one keratinocyte cell line via flow cytometry. These cell lines were treated with Fas ligand-Fc (FasL) and birinapant, a bivalent SMAC mimetic, in mono and combination therapies...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29467325/lipid-abnormalities-in-atopic-skin-are-driven-by-type-2-cytokines
#6
Evgeny Berdyshev, Elena Goleva, Irina Bronova, Nathan Dyjack, Cydney Rios, John Jung, Patricia Taylor, Mingeum Jeong, Clifton F Hall, Brittany N Richers, Kathryn A Norquest, Tao Zheng, Max A Seibold, Donald Ym Leung
Lipids in the stratum corneum of atopic dermatitis (AD) patients differ substantially in composition from healthy subjects. We hypothesized that hyperactivated type 2 immune response alters AD skin lipid metabolism. We have analyzed stratum corneum lipids from nonlesional and lesional skin of AD subjects and IL-13 skin-specific Tg mice. We also directly examined the effects of IL-4/IL-13 on human keratinocytes in vitro. Mass spectrometric analysis of lesional stratum corneum from AD subjects and IL-13 Tg mice revealed an increased proportion of short-chain (N-14:0 to N-24:0) NS ceramides, sphingomyelins, and 14:0-22:0 lysophosphatidylcholines (14:0-22:0 LPC) with a simultaneous decline in the proportion of corresponding long-chain species (N-26:0 to N-32:0 sphingolipids and 24:0-30:0 LPC) when compared with healthy controls...
February 22, 2018: JCI Insight
https://www.readbyqxmd.com/read/29464051/human-papillomavirus-e7-oncoprotein-expression-by-keratinocytes-alters-the-cytotoxic-mechanisms-used-by-cd8-t-cells
#7
Purnima Bhat, Anne-Sophie Bergot, Nigel Waterhouse, Ian Hector Frazer
Cervical cancer is a malignant transformation of keratinocytes initiated by the E7 oncoprotein of human papillomavirus (HPV). These tumors are characterized by keratinocyte hyperproliferation and are often infiltrated with activated CD8 T cells. HPV infection confers changes to gain immunological advantage to promote chronic infection, and these persist with malignant transformation. We investigated the relative importance of the many redundant mechanisms of cytotoxicity used by CD8 T cells to kill keratinocytes expressing HPV E7 oncoprotein using extended-duration time-lapse microscopy that allows examination of cell-to-cell interactions during killing...
January 19, 2018: Oncotarget
https://www.readbyqxmd.com/read/29463581/a-novel-mechanism-for-activation-of-gli1-by-nuclear-smo-that-escapes-anti-smo-inhibitors
#8
Muhammad M Rahman, Allon Hazan, Joanne L Selway, Dimalee S Herath, Catherine A Harwood, Muhammad S Pirzado, Ravinder Atkar, David P Kelsell, Kenneth J Linton, Mike P Philpott, Graham W Neill
Small molecule inhibitors of the Hedgehog (HH) pathway receptor Smoothened (SMO) have been effective in treating some patients with basal cell carcinoma (BCC), where the HH pathway is often activated, but many patients are poorly responsive. In this study, we report the results of investigations on PTCH1 signalling in the HH pathway that suggest why most BCC patients respond poorly to SMO inhibitors. In immortalised human keratinocytes, PTCH1 silencing led to the generation of a compact, holoclone-like morphology with increased expression of SMO and the downstream HH pathway transcription factor GLI1...
February 20, 2018: Cancer Research
https://www.readbyqxmd.com/read/29463027/the-connexin-mimetic-peptide-gap27-and-cx43-knockdown-reveal-differential-roles-for-connexin43-in-wound-closure-events-in-skin-model-systems
#9
Chrysovalantou Faniku, Erin O'Shaughnessy, Claire Lorraine, Scott R Johnstone, Annette Graham, Sebastian Greenhough, Patricia E M Martin
In the epidermis, remodelling of Connexin43 is a key event in wound closure. However, controversy between the role of connexin channel and non-channel functions exist. We compared the impact of SiRNA targeted to Connexin43 and the connexin mimetic peptide Gap27 on scrape wound closure rates and hemichannel signalling in adult keratinocytes (AK) and fibroblasts sourced from juvenile foreskin (JFF), human neonatal fibroblasts (HNDF) and adult dermal tissue (ADF). The impact of these agents, following 24 h exposure, on GJA1 (encoding Connexin43), Ki67 and TGF-β1 gene expression, and Connexin43 and pSmad3 protein expression levels, were examined by qPCR and Western Blot respectively...
February 18, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29462589/potentiation-of-skin-tslp-production-by-a-cosmetic-colorant-leads-to-aggravation-of-dermatitis-symptoms
#10
Gabsik Yang, Hye Eun Lee, Kyung-Min Lim, Yong-Kyu Choi, Kyu-Bong Kim, Byung-Mu Lee, Joo Young Lee
Certain cosmetic colorants are irritant to skin or aggravate dermatitis. Thymic stromal lymphopoietin (TSLP) plays an important role in the initiation and progress of skin inflammation and atopic dermatitis by triggering Th2 immune responses. However, the effects of cosmetic colorants on TSLP production are unknown yet. Therefore, we investigated whether cosmetic colorants regulated TSLP production and dermatitis. Lithol Rubine B (LR-B, Pigment Red 57) and its calcium salt (LR-BCA), commonly used cosmetic colorants, potentiated phorbol-12-myristate-13-acetate-induced TSLP production in keratinocytes...
February 17, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29462476/malassezia-pachydermatis-up-regulates-ahr-related-cyp1a1-gene-and-epidermal-barrier-markers-in-human-keratinocytes
#11
Elisabetta Buommino, Adone Baroni, Chiara Papulino, Francesca Paola Nocera, Lorena Coretti, Giovanna Donnarumma, Anna De Filippis, Luisa De Martino
Cytochrome P450 CYP1A1 and CYP1B1 enzymes are regulated by the aryl hydrocarbon receptor (AhR), a transcription factor activated by a variety of ligands among which Malassezia metabolites. In this study, we analyzed the modulation of CYP1A1, CYP1B1, and AhR in human keratinocytes infected with different strains of Malassezia pachydermatis, as well as the upregulation of some genes involved in the epidermal homeostasis. We demonstrated that all the strains induced AhR activation and its nuclear translocation in HaCaT cells infected for 24 h, compared to untreated cells...
February 15, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29460532/lichen-planus-molecular-pathway-and-clinical-implications-in-oral-disorders
#12
M Boccellino, D Di Stasio, A Romano, M Petruzzi, A Lucchese, R Serpico, L Frati, M Di Domenico
Stem cells play a role in many mucosal disorders characterised by abnormal proliferation and differentiation of keratinocytes, such as oral lichen planus (OLP). In OLP there were changes in stem cell markers as component of integrin complexes α6 and β1 integrin increased along with increase of melanoma-associated chondroitin sulphate proteoglycan (MCSP) and decreased of notch1 (N1) and keratin 15 (K15). Stem cell marker expression may be altered by pathological signalling in these lesions. Cadherins are transmembrane receptors that provide cell-cell contact and communication function through calcium-dependent homophilic and heterophilic interactions...
January 2018: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/29459885/platelet-activating-factor-receptor-ligands-protect-tumor-cells-from-radiation-induced-cell-death
#13
Ildefonso Alves da Silva-Junior, Barbara Dalmaso, Suellen Herbster, Ana Paula Lepique, Sonia Jancar
Irradiation generates oxidized phospholipids that activate platelet-activating factor receptor (PAFR) associated with pro-tumorigenic effects. Here, we investigated the involvement of PAFR in tumor cell survival after irradiation. Cervical cancer samples presented higher levels of PAF-receptor gene (PTAFR) when compared with normal cervical tissue. In cervical cancer patients submitted to radiotherapy (RT), the expression of PTAFR was significantly increased. Cervical cancer-derived cell lines (C33, SiHa, and HeLa) and squamous carcinoma cell lines (SCC90 and SCC78) express higher levels of PAFR mRNA and protein than immortalized keratinocytes...
2018: Frontiers in Oncology
https://www.readbyqxmd.com/read/29459852/%C3%AE-hpv-infection-correlates-with-early-stages-of-carcinogenesis-in-skin-tumors-and-patient-derived-xenografts-from-a-kidney-transplant-recipient-cohort
#14
Cinzia Borgogna, Carlotta Olivero, Simone Lanfredini, Federica Calati, Marco De Andrea, Elisa Zavattaro, Paola Savoia, Elena Trisolini, Renzo Boldorini, Girish K Patel, Marisa Gariglio
Many malignancies that occur in high excess in kidney transplant recipients (KTRs) are due to viruses that thrive in the setting of immunosuppression. Keratinocyte carcinoma (KC), the most frequently occurring cancer type in KTR, has been associated with skin infection by human papillomavirus (HPV) from the beta genus. In this report, we extend our previous investigation aimed at identifying the presence of active β-HPV infection in skin tumors from KTRs through detection of viral protein expression. Using a combination of antibodies raised against the E4 and L1 proteins of the β-genotypes, we were able to visualize infection in five tumors [one keratoacanthoma (KA), three actinic keratoses (AKs), and one seborrheic keratoses (SKs)] that were all removed from two patients who had been both transplanted twice, had developed multiple KCs, and presented with a long history of immunosuppression (>30 years)...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29458675/the-contribution-of-sp100-to-cottontail-rabbit-papillomavirus-transcription-and-replication
#15
M Wurdak, M Schneider, T Iftner, F Stubenrauch
SP100 proteins are components of nuclear domain 10 structures and have been implicated as inhibitors of human papillomavirus (HPV) replication. In this study, we have addressed the role of SP100 in tumour formation by the cottontail rabbit (Sylvilagus floridanus) papillomavirus (CRPV or SfPV1) in a rabbit model. Tissue culture studies using rabbit keratinocyte lines indicated that rabbit SP100 is an interferon-beta-inducible gene similar to its human counterpart. Stable knockdown of SP100 by shRNA in a cell line harbouring CRPV genomes resulted in a decrease of viral early transcripts...
January 24, 2018: Journal of General Virology
https://www.readbyqxmd.com/read/29458673/denture-associated-biofilm-infection-in-three-dimensional-oral-mucosal-tissue-models
#16
Daniel J Morse, Melanie J Wilson, Xiaoqing Wei, Michael A O Lewis, David J Bradshaw, Craig Murdoch, David W Williams
PURPOSE: In vitro analyses of virulence, pathogenicity and associated host cell responses are important components in the study of biofilm infections. The Candida-related infection, denture-associated oral candidosis, affects up to 60 % of denture wearers and manifests as inflammation of palatal tissues contacting the denture-fitting surface. Commercially available three-dimensional tissue models can be used to study infection, but their use is limited for many academic research institutions, primarily because of the substantial purchase costs...
January 11, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29458120/major-role-for-trpv1-and-insp3r-in-par-2-elicited-inflammatory-mediator-production-in-differentiated-human-keratinocytes
#17
Olivier Gouin, Killian L'Herondelle, Paul Buscaglia, Christelle Le Gall-Ianotto, Réginald Philippe, Nelig Legoux, Olivier Mignen, Virginie Buhé, Raphael Leschiera, Mehdi Sakka, Nathalie Kerfant, Jean-Luc Carré, Raphaele Le Garrec, Luc Lefeuvre, Nicolas Lebonvallet, Laurent Misery
Proteinase-activated receptor-2 (PAR-2) activation in basal keratinocytes stimulates inflammation via the Ca2+ -dependent production of mediators such as interleukin (IL)-1β, tumor necrosis factor (TNF)-α and thymic stromal lymphopoietin (TSLP). In this study, we investigated PAR-2 calcium signaling and the consequent production of inflammatory mediators in differentiated human primary keratinocytes (DhPKs). Stimulation with the PAR-2 activating peptide SLIGKV promoted Ca2+ store depletion in both undifferentiated human primary keratinocytes (UhPKs) and DhPKs...
February 16, 2018: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/29454234/diarylheptanoid-from-rhizomes-of-curcuma-kwangsiensis-dck-inhibited-imiquimod-induced-dendritic-cells-activation-and-th1-th17-differentiation
#18
Qing Liu, Wei Yin, Ling Han, Jiaoyan Lv, Bingji Li, Yuli Lin, Qingsheng Mi, Rui He, Chuanjian Lu
BACKGROUND AND OBJECT: Dendritic cells (DCs) are critical for initiating the activation and differentiation of T cells in inflammatory diseases including psoriasis. Curcuma kwangsiensis S.G. Lee & C.F. Liang is a herb for treating psoriasis and we previously found Diarylheptanoid from rhizomes of Curcuma kwangsiensis (DCK) inhibited keratinocytes proliferation. However, it is unknown whether DCK influences DC functions. Thus we aimed to explore whether DCK affect the major immunological functions of DCs...
February 14, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29452884/water-soluble-glucose-derivative-of-thiocarbohydrazone-acts-as-ionophore-with-cytotoxic-effects-on-tumor-cells
#19
Carmela Bonaccorso, Giulia Grasso, Nicolò Musso, Vincenza Barresi, Daniele F Condorelli, Diego La Mendola, Enrico Rizzarelli
A novel water-soluble ionophore based on the thiocarbohydrazone moiety conjugated with glucose (GluTch) was synthesized through a simple two-step procedure. Structural elucidation was carried out in water solution by means of various spectroscopic techniques (NMR, UV-Vis, and CD), electrospray ionization mass spectrometry and density functional theory calculations. The flexible nature of the thiocarbohydrazone moiety of the new glycoderivative compound induced both different coordination motifs and stoichiometry towards copper and zinc...
February 9, 2018: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29452119/mirs-103-107-regulate-autophagy-in-the-epidermis
#20
Sijia Wang, Aya Kobeissi, Ying Dong, Nihal Kaplan, Wending Yang, Congcong He, Kang Zeng, Han Peng
We have shown that microRNAs-103 and -107(miRs-103/107) positively regulate end-stage autophagy via ensuring dynamin activity in cultured keratinocytes. Most work in end-stage autophagy has been conducted using in vitro model systems. In vivo regulation of end-stage autophagy in epidermis remains unknown. Here, we used antagomirs to subcutaneously knock-down miR-107 in the skin; conversely, we delivered miR-107 mimic subcutaneously via in vivo transfection to increase this miRNA. We found that antagomir-107 treatment in epidermis: (i) depleted endogenous miR-107; (ii) increased GFP-LC3 puncta in epidermal basal layers of GFP-LC3 transgenic mice, indicative of an accumulation of autophagosomes; (iii) inhibited LC3 turnover and increased p62, suggesting an inhibition of autophagy flux; and (iv) increased phosphorylated dynamin (p-dynamin, an inactive form), a key enzyme in end-stage autophagy...
February 13, 2018: Journal of Investigative Dermatology
keyword
keyword
61959
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"