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https://www.readbyqxmd.com/read/27896242/intracytoplasmic-sperm-injection-outcomes-with-freshly-ejaculated-sperms-and-testicular-or-epididymal-sperm-extraction-in-patients-with-idiopathic-cryptozoospermia
#1
Ali Asghar Ketabchi
BACKGROUND: Cryptozoospermia (CO) is a situation in which spermatozoa cannot be observed in a fresh semen sample unless an extended centrifugation and microscopic search are performed. CO patients are suggested to use only intracytoplasmic sperm injection (ICSI) as infertility treatment. But still there is debate about the choice of sperm source in cryptozoospermic men candidate for ICSI. OBJECTIVES: This study was conducted to evaluate fertility outcomes in men with idiopathic cryptozoospermia who were treated using ICSI with freshly ejaculated sperm and testis sperm extraction (TESE) or percutaneous epididymal sperm aspiration (PESA)...
November 2016: Nephro-urology Monthly
https://www.readbyqxmd.com/read/27894976/comparison-of-the-clinical-outcomes-of-intracytoplasmic-sperm-injection-between-spermatozoa-retrieved-from-testicular-biopsy-and-from-ejaculate-in-cryptozoospermia-patients
#2
Xianfeng Cui, Pan Ding, Ge Gao, Yunshan Zhang
OBJECTIVE: To compare the clinical outcomes of intracytoplasmic sperm injection (ICSI) between spermatozoa retrieved from testicular biopsy and from ejaculate in cryptozoospermia patients. METHODS: The clinical data of 285 cryptozoospermia patients, who underwent ICSI treatment in our center during the period from March 2009 to November 2013, were retrospectively analyzed. Within them, ejaculated sperms were used in 214 cases (group 1) and testicular sperms extracted by testicular sperm aspiration (TESA) or conventional testis dissection sperm extraction (TESE) were used in 71 cases (group 2)...
November 25, 2016: Urology
https://www.readbyqxmd.com/read/27833987/pregnancy-resulting-from-imsi-after-testicular-biopsy-in-a-patient-with-obstructive-azoospermia
#3
Constantin Cristian Văduva, Carmen Constantinescu, Mihaela Mirela Radu, Alice Roxana Văduva, Andrei Pănuş, Mihaela Ţenovici, Damian DiŢescu, Dinu Florin Albu
In this article, we report a case of pregnancy obtained in an infertile couple diagnosed with severe male factor infertility. The couple attended for fertility examination reporting a history of 10 years of infertility. The cause of infertility was obstructive azoospermia. The treatment consists of in vitro fertilization (IVF). The ovarian stimulation of female patient was done with antagonist protocol and after ovarian puncture was obtained nine oocytes. The urologist performed testicular sperm extraction (TESE)...
2016: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/27793367/behavioral-cognitive-and-motor-performance-and-physical-development-of-five-year-old-children-who-were-born-after-intracytoplasmic-sperm-injection-with-the-use-of-testicular-sperm
#4
Aukje M Meijerink, Liliana Ramos, Anjo J W M Janssen, Nienke M Maas-van Schaaijk, Andreas Meissner, Sjoerd Repping, Monique H Mochtar, Didi D M Braat, Kathrin Fleischer
OBJECTIVE: To evaluate at the age of 5 years the behavioral, cognitive, and motor performance and physical development of children born after testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI). DESIGN: A prospective longitudinal cohort study. SETTING: Two university medical centers. PATIENT(S): A total of 103 5-year-olds who were born after TESE-ICSI. INTERVENTION(S): The follow-up of the children was performed by questionnaires at birth and again at 1 year and at 4 years of age...
December 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27785898/outcome-of-intracytoplasmic-sperm-injection-using-fresh-and-cryopreserved-thawed-test%C3%A4-cular-spermatozoa-in-83-azoospermic-men-with-klinefelter-syndrome
#5
Kubilay Vicdan, Cem Akarsu, Eran Sözen, Burcu Buluç, Arzu Vicdan, Yıldırım Yılmaz, Kutay Biberoğlu
AIM: To report the outcome of intracytoplasmic sperm injection (ICSI) cycles using fresh or cryopreserved-thawed testicular spermatozoa of men with Klinefelter syndrome (KS). METHODS: Medical records of 83 azoospermic men with KS who underwent testicular sperm extraction (TESE) were reviewed. The clinical parameters for predicting sperm retrieval and fertilization, implantation, pregnancy and live birth rates of ICSI cycles in these patients were evaluated. RESULTS: A total of 88 TESE procedures were performed with sperm retrieval rates of 39...
November 2016: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/27646677/improving-icsi-a-review-from-the-spermatozoon-perspective
#6
Mara Simopoulou, Laertis Gkoles, Panagiotis Bakas, Polina Giannelou, Theodoros Kalampokas, Konstantinos Pantos, Michael Koutsilieris
: Intracytoplasmic sperm injection (ICSI) is the most frequently applied method for fertilization making the process of identifying the perfect spermatozoon fundamental. Herein we offer a critical and thorough presentation on the techniques reported regarding (i) handling and preparing semen samples, (ii) identifying and 'fishing' spermatozoa, and (iii) improving key factors, such as motility for a successful ICSI practice. These approaches are suggested to make the process easier and more effective especially in atypical and challenging circumstances...
December 2016: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/27538137/-outcome-of-treatment-of-y-chromosome-azfc-microdeletion-patients
#7
L M Zhao, H Jiang, K Hong, H C Lin, W H Tang, D F Liu, J M Mao, Y Lian, L L Ma
OBJECTIVE: To discuss the treatment options for patients with azoospermia factor (AZF) c microdeletion on Y chromosome. METHODS: One hundred and eighty three patients, who were diagnosed as AZFc microdeletion on Y chromosome in Peking University Third Hospital, were recruited in our study. In order to get better treatment option for this kind of patients, we retrospectively analyzed their clinic data including the treatment process and pregnancy outcome and found out the characteristics of their semen...
February 18, 2016: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/27526630/undergoing-varicocele-repair-before-assisted-reproduction-improves-pregnancy-rate-and-live-birth-rate-in-azoospermic-and-oligospermic-men-with-a-varicocele-a-systematic-review-and-meta-analysis
#8
E Will Kirby, Laura Elizabeth Wiener, Saneal Rajanahally, Karen Crowell, Robert M Coward
OBJECTIVE: To evaluate how varicocele repair (VR) impacts pregnancy (PRs) and live birth rates in infertile couples undergoing assisted reproduction wherein the male partner has oligospermia or azoospermia and a history of varicocele. DESIGN: Systematic review and meta-analysis. SETTING: Not applicable. PATIENT(S): Azoospermic and oligospermic males with varicoceles and in couples undergoing assisted reproductive technology (ART) with IUI, IVF, or testicular sperm extraction (TESE) with IVF and intracytoplasmic sperm injection (ICSI)...
August 12, 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27416674/-predictive-factors-of-testicular-sperm-extraction-in-men-with-non-obstructive-azoospermia
#9
REVIEW
Huan-li Yang, Xiu-juan Shao, Yi-yang Zhu, Wei-ling Wu
Men with non-obstructive azoospermia (NOA) can achieve fertility by testicular sperm extraction (TESE) coupled with intracytoplasmic sperm injection (ICSI), the key to which is the successful retrieval of sperm from the testis. Although improved testicular sperm extraction techniques have increased the chances of sperm retrieval, to predict preoperatively the success of sperm retrieval from NOA patients remains challenging. A non-invasive diagnostic technique predicting the presence of sperm in the testis would be useful for avoiding possible surgical intervention...
May 2016: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/27406950/prediction-model-for-obtaining-spermatozoa-with-testicular-sperm-extraction-in-men-with-non-obstructive-azoospermia
#10
M Cissen, A M Meijerink, K W D'Hauwers, A Meissner, N van der Weide, M H Mochtar, A A de Melker, L Ramos, S Repping, D D M Braat, K Fleischer, M van Wely
STUDY QUESTION: Can an externally validated model, based on biological variables, be developed to predict successful sperm retrieval with testicular sperm extraction (TESE) in men with non-obstructive azoospermia (NOA) using a large nationwide cohort? SUMMARY ANSWER: Our prediction model including six variables was able to make a good distinction between men with a good chance and men with a poor chance of obtaining spermatozoa with TESE. WHAT IS KNOWN ALREADY: Using ICSI in combination with TESE even men suffering from NOA are able to father their own biological child...
September 2016: Human Reproduction
https://www.readbyqxmd.com/read/27406949/prediction-model-for-live-birth-in-icsi-using-testicular-extracted-sperm
#11
A M Meijerink, M Cissen, M H Mochtar, K Fleischer, I Thoonen, A A de Melker, A Meissner, S Repping, D D M Braat, M van Wely, L Ramos
STUDY QUESTION: Which parameters have a predictive value for live birth in couples undergoing ICSI after successful testicular sperm extraction (TESE-ICSI)? SUMMARY ANSWER: Female age, a first or subsequent started TESE-ICSI cycle, male LH, male testosterone, motility of the spermatozoa during the ICSI procedure and the initial male diagnosis before performing TESE were identified as relevant and independent parameters for live birth after TESE-ICSI. WHAT IS KNOWN ALREADY: In reproductive medicine prediction models are used frequently to predict treatment success, but no prediction model currently exists for live birth after TESE-ICSI...
September 2016: Human Reproduction
https://www.readbyqxmd.com/read/27306218/microdissection-testicular-sperm-extraction-in-japanese-patients-with-persistent-azoospermia-after-chemotherapy
#12
Takeshi Shin, Tomohiro Kobayashi, Yukihito Shimomura, Toshiyuki Iwahata, Keisuke Suzuki, Takashi Tanaka, Mai Fukushima, Megumi Kurihara, Akane Miyata, Yoshitomo Kobori, Hiroshi Okada
BACKGROUND: Combinations of surgery, radiation therapy, and chemotherapy can achieve high remission rates in patients with cancer, but these treatments can have damaging effects on spermatogenesis. In particular, cytotoxic chemotherapy may lead to irreversible spermatogenic dysfunction. Microdissection testicular sperm extraction (micro-TESE) is the only method that can address infertility in cancer survivors with persistent postchemotherapy azoospermia. METHODS: We included 66 Japanese patients with postchemotherapy azoospermia who underwent micro-TESE for sperm retrieval in this analysis...
December 2016: International Journal of Clinical Oncology
https://www.readbyqxmd.com/read/27231097/intervention-improves-assisted-conception-intracytoplasmic-sperm-injection-outcomes-for-patients-with-high-levels-of-sperm-dna-fragmentation-a-retrospective-analysis
#13
C K Bradley, S J McArthur, A J Gee, K A Weiss, U Schmidt, L Toogood
Sperm DNA fragmentation (SDF) is used in assisted reproductive technology (ART) programs as an indicator for sperm quality, although there is still a lack of consensus as to its clinical utility. In this retrospective study, we examined intracytoplasmic sperm injection (ICSI) outcomes of 1924 infertile patients who underwent SDF analysis using the sperm chromatin integrity test. ART patients were classified as having low [DNA fragmentation index (DFI) <29%] or high SDF (DFI ≥29%) and by whether or not an intervention [physiological intracytoplasmic sperm injection (PICSI), intracytoplasmic morphologically selected sperm injection (IMSI), testicular sperm extraction (TESE)/testicular sperm aspiration (TESA), frequent ejaculation] was performed...
September 2016: Andrology
https://www.readbyqxmd.com/read/27198124/icsi-outcomes-in-men-undergoing-tese-for-azoospermia-and-impact-of-maternal-age
#14
S Bocca, V Moussavi, V Brugh, M Morshedi, L Stadtmauer, S Oehninger
This retrospective study compared clinical outcomes in men with obstructive and nonobstructive azoospermia after ICSI following testicular sperm extraction and the influence of maternal age. Fertilisation rates, embryo quality, pregnancy rates, miscarriage rates and live birth rates were evaluated. Men with obstructive azoospermia (OA) had significantly higher rates of diploid fertilisation and clinical pregnancy than men with nonobstructive azoospermia (NOA), but miscarriage rates and live birth rates were not significantly different...
May 20, 2016: Andrologia
https://www.readbyqxmd.com/read/27176005/a-novel-stepwise-micro-tese-approach-in-non-obstructive-azoospermia
#15
Giorgio Franco, Filomena Scarselli, Valentina Casciani, Cosimo De Nunzio, Donato Dente, Costantino Leonardo, Pier Francesco Greco, Alessia Greco, Maria Giulia Minasi, Ermanno Greco
BACKGROUND: The purpose of the study was to investigate whether micro-TESE can improve sperm retrieval rate (SRR) compared to conventional single TESE biopsy on the same testicle or to contralateral multiple TESE, by employing a novel stepwise micro-TESE approach in a population of poor prognosis patients with non-obstructive azoospermia (NOA). METHODS: Sixty-four poor prognosis NOA men undergoing surgical testicular sperm retrieval for ICSI, from March 2007 to April 2013, were included in this study...
2016: BMC Urology
https://www.readbyqxmd.com/read/27130613/couples-with-non-obstructive-azoospermia-are-interested-in-future-treatments-with-artificial-gametes
#16
S Hendriks, M Hessel, M H Mochtar, A Meissner, F van der Veen, S Repping, E A F Dancet
STUDY QUESTION: Would couples diagnosed with non-obstructive azoospermia (NOA) consider two future treatments with artificial gametes (AGs) as alternatives for testicular sperm extraction followed by ICSI (TESE-ICSI)? SUMMARY ANSWER: Most couples with NOA (89%) would opt for treatment with AGs before attempting TESE-ICSI and/or after failed TESE-ICSI. WHAT IS KNOWN ALREADY: Couples with NOA who undergo TESE-ICSI have a 25% chance of conceiving a child...
August 2016: Human Reproduction
https://www.readbyqxmd.com/read/27107595/in-vitro-spermatogenesis-how-far-from-clinical-application
#17
REVIEW
Guillermo Galdon, Anthony Atala, Hooman Sadri-Ardekani
Male infertility affects 7 % of the male population, and 10 % of infertile men are azoospermic. In these instances, using microsurgical testicular sperm extraction (m-TESE) and intra-cytoplasmic sperm injection (ICSI) helps a significant number of patients. However, in vitro differentiation of diploid germ cells to mature haploid germ cell has the potential to benefit many others, including pediatric cancer survivors who have previously cryopreserved their immature testicular tissue prior to starting gonadotoxic cancer treatment as well as men with spermatogenic arrest...
July 2016: Current Urology Reports
https://www.readbyqxmd.com/read/27104311/microfluidics-the-future-of-microdissection-tese
#18
Raheel Samuel, Odgerel Badamjav, Kristin E Murphy, Darshan P Patel, Jiyoung Son, Bruce K Gale, Douglas T Carrell, James M Hotaling
Non-obstructive azoospermia (NOA) is a severe form of infertility accounting for 10% of infertile men. Microdissection testicular sperm extraction (microTESE) includes a set of clinical protocols from which viable sperm are collected from patients (suffering from NOA), for intracytoplasmic sperm injection (ICSI). Clinical protocols associated with the processing of a microTESE sample are inefficient and significantly reduce the success of obtaining a viable sperm population. In this review we highlight the sources of these inefficiencies and how these sources can possibly be removed by microfluidic technology and single-cell Raman spectroscopy...
June 2016: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/27062982/generation-of-hprt-disrupted-rat-through-mouse%C3%A2-rat-es-chimeras
#19
Ayako Isotani, Kazuo Yamagata, Masaru Okabe, Masahito Ikawa
We established rat embryonic stem (ES) cell lines from a double transgenic rat line which harbours CAG-GFP for ubiquitous expression of GFP in somatic cells and Acr3-EGFP for expression in sperm (green body and green sperm: GBGS rat). By injecting the GBGS rat ES cells into mouse blastocysts and transplanting them into pseudopregnant mice, rat spermatozoa were produced in mouse←rat ES chimeras. Rat spermatozoa from the chimeric testis were able to fertilize eggs by testicular sperm extraction combined with intracytoplasmic sperm injection (TESE-ICSI)...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26908064/y-chromosome-microdeletions-in-nonobstructive-azoospermia-and-severe-oligozoospermia
#20
Carolina Goncalves, Mariana Cunha, Eduardo Rocha, Susana Fernandes, Joaquina Silva, LuIs Ferraz, Cristiano Oliveira, Alberto Barros, Mário Sousa
The aim of the present work was to present the outcomes of the patients with Y-chromosome microdeletions treated by intracytoplasmic sperm injection (ICSI), either using fresh (TESE) or frozen-thawed (TESE-C) testicular sperm and ejaculated sperm (EJAC). The originality of this work resides in the comparisons between the different types of Y-microdeletions (AZFa, AZFb, and AZFc) and treatments, with detailed demographic, stimulation, embryological, clinical, and newborn (NB) outcomes. Of 125 patients with Y-microdeletions, 33 patients presented severe oligozoospermia (18 performed ICSI with ejaculated sperm) and 92 secretory azoospermia (65 went for TESE with 40 having successful sperm retrieval and performed ICSI)...
February 23, 2016: Asian Journal of Andrology
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