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https://www.readbyqxmd.com/read/27904772/mir-101-targets-usp22-to-inhibit-the-tumorigenesis-of-papillary-thyroid-carcinoma
#1
Huadong Zhao, Haili Tang, Qike Huang, Bo Qiu, Xiaomin Liu, Dong Fan, Li Gong, Hang Guo, Chong Chen, Shixiong Lei, Lu Yang, Jianguo Lu, Guoqiang Bao
Increasing evidence suggests that microRNA-101 (miR-101) is involved in the progression of various human cancers, including papillary thyroid carcinoma (PTC). However, the biological functions of miR-101 and underlying molecular mechanisms in PTC remain largely unknown. In this study, we demonstrated that miR-101 underexpression in PTC tissue was associated with lymph node metastasis and poor prognosis of PTC patients. MiR-101 reduced PTC cell proliferation, apoptosis resistance, and invasion. Ubiquitin-specific protease 22 (USP22) was confirmed as a direct target of miR-101...
2016: American Journal of Cancer Research
https://www.readbyqxmd.com/read/27601583/cytoplasmic-atxn7l3b-interferes-with-nuclear-functions-of-the-saga-deubiquitinase-module
#2
Wenqian Li, Boyko S Atanassov, Xianjiang Lan, Ryan D Mohan, Selene K Swanson, Aimee T Farria, Laurence Florens, Michael P Washburn, Jerry L Workman, Sharon Y R Dent
The SAGA complex contains two enzymatic modules, which house histone acetyltransferase (HAT) and deubiquitinase (DUB) activities. USP22 is the catalytic subunit of the DUB module, but two adaptor proteins, ATXN7L3 and ENY2, are necessary for DUB activity toward histone H2Bub1 and other substrates. ATXN7L3B shares 74% identity with the N-terminal region of ATXN7L3, but the functions of ATXN7L3B are not known. Here we report that ATXN7L3B interacts with ENY2 but not other SAGA components. Even though ATXN7L3B localizes in the cytoplasm, ATXN7L3B overexpression increases H2Bub1 levels, while overexpression of ATXN7L3 decreases H2Bub1 levels...
November 15, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27501329/nuclear-gsk3%C3%AE-promotes-tumorigenesis-by-phosphorylating-kdm1a-and-inducing-its-deubiquitylation-by-usp22
#3
Aidong Zhou, Kangyu Lin, Sicong Zhang, Yaohui Chen, Nu Zhang, Jianfei Xue, Zhongyong Wang, Kenneth D Aldape, Keping Xie, James R Woodgett, Suyun Huang
Emerging evidence has shown that GSK3β plays oncogenic roles in multiple tumour types; however, the underlying mechanisms remain largely unknown. Here, we show that nuclear GSK3β is responsible for the accumulation of the histone demethylase KDM1A and critically regulates histone H3K4 methylation during tumorigenesis. GSK3β phosphorylates KDM1A Ser683 upon priming phosphorylation of KDM1A Ser687 by CK1α. Phosphorylation of KDM1A induces its binding with and deubiquitylation by USP22, leading to KDM1A stabilization...
September 2016: Nature Cell Biology
https://www.readbyqxmd.com/read/27160905/the-saga-deubiquitination-module-promotes-dna-repair-and-class-switch-recombination-through-atm-and-dnapk-mediated-%C3%AE-h2ax-formation
#4
Shaliny Ramachandran, Dania Haddad, Conglei Li, Michael X Le, Alexanda K Ling, Clare C So, Rajeev M Nepal, Jennifer L Gommerman, Kefei Yu, Troy Ketela, Jason Moffat, Alberto Martin
Class switch recombination (CSR) requires activation-induced deaminase (AID) to instigate double-stranded DNA breaks at the immunoglobulin locus. DNA breaks activate the DNA damage response (DDR) by inducing phosphorylation of histone H2AX followed by non-homologous end joining (NHEJ) repair. We carried out a genome-wide screen to identify CSR factors. We found that Usp22, Eny2, and Atxn7, members of the Spt-Ada-Gcn5-acetyltransferase (SAGA) deubiquitination module, are required for deubiquitination of H2BK120ub following DNA damage, are critical for CSR, and function downstream of AID...
May 17, 2016: Cell Reports
https://www.readbyqxmd.com/read/27145278/targeting-ubiquitin-specific-protease-22-suppresses-growth-and-metastasis-of-anaplastic-thyroid-carcinoma
#5
Hua-Dong Zhao, Hai-Li Tang, Ning-Ning Liu, Ya-Li Zhao, Qin-Qin Liu, Xiao-Shan Zhu, Lin-Tao Jia, Chun-Fang Gao, An-Gang Yang, Jun-Tang Li
Ubiquitin-specific protease 22 (USP22) aberrance has been implicated in several malignancies; however, whether USP22 plays a role in anaplastic thyroid carcinoma (ATC) remains unclear. Here, we report that USP22 expression is highly elevated in ATC tissues, which positively correlated with tumor size, extracapsular invasion, clinical stages, and poor prognosis of ATC patients. In vitro assays showed that USP22 depletion suppressed ATC cell survival and proliferation by decreasing Rb phosphorylation and cyclin D2, inactivating Akt, and simultaneously upregulating Rb; USP22 silencing restrained cell migration and invasion by inhibiting epithelial-mesenchymal transition; USP22 knockdown promoted mitochondrion- mediated and caspase-dependent apoptosis by upregulating Bax and Bid and promoting caspase-3 activation...
May 24, 2016: Oncotarget
https://www.readbyqxmd.com/read/27132940/atxn7l3-and-eny2-coordinate-activity-of-multiple-h2b-deubiquitinases-important-for-cellular-proliferation-and-tumor-growth
#6
Boyko S Atanassov, Ryan D Mohan, Xianjiang Lan, Xianghong Kuang, Yue Lu, Kevin Lin, Elizabeth McIvor, Wenqian Li, Ying Zhang, Laurence Florens, Stephanie D Byrum, Samuel G Mackintosh, Tammy Calhoun-Davis, Evangelia Koutelou, Li Wang, Dean G Tang, Alan J Tackett, Michael P Washburn, Jerry L Workman, Sharon Y R Dent
Histone H2B monoubiquitination (H2Bub1) is centrally involved in gene regulation. The deubiquitination module (DUBm) of the SAGA complex is a major regulator of global H2Bub1 levels, and components of this DUBm are linked to both neurodegenerative diseases and cancer. Unexpectedly, we find that ablation of USP22, the enzymatic center of the DUBm, leads to a reduction, rather than an increase, in global H2bub1 levels. In contrast, depletion of non-enzymatic components, ATXN7L3 or ENY2, results in increased H2Bub1...
May 19, 2016: Molecular Cell
https://www.readbyqxmd.com/read/27057639/ubiquitin-specific-peptidase-22-functions-and-its-involvement-in-disease
#7
Johanna Melo-Cardenas, Yusi Zhang, Donna D Zhang, Deyu Fang
Deubiquitylases remove ubiquitin moieties from different substrates to regulate protein activity and cell homeostasis. Since this posttranslational modification plays a role in several different cellular functions, its deregulation has been associated with different pathologies. Aberrant expression of the Ubiquitin-Specific Peptidase 22 (USP22) has been associated with poor cancer prognosis and neurological disorders. However, little is known about USP22 role in these pathologies or in normal physiology. This review summarizes the current knowledge about USP22 function from yeast to human and provides an overview of the possible mechanisms by which USP22 is emerging as a potential oncogene...
April 5, 2016: Oncotarget
https://www.readbyqxmd.com/read/27051294/mir-34b-inhibits-nasopharyngeal-carcinoma-cell-proliferation-by-targeting-ubiquitin-specific-peptidase-22
#8
Jianyong Xiao, Yingying Li, Wenyin Zhang, Yanni Jiang, Biaoyan Du, Yuhui Tan
OBJECTIVES: This study aimed to investigate the precise role of miR-34b in nasopharyngeal carcinoma (NPC). MATERIALS AND METHODS: The expression of miR-34b and transcription of ubiquitin-specific peptidase 22 (USP22) were examined using quantitative reverse transcription-polymerase chain reaction. Western blot analysis was used to measure the protein expression of USP22. A dualluciferase assay was used to investigate the interaction between miR-34b and USP22. Cell viability was determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay...
2016: OncoTargets and Therapy
https://www.readbyqxmd.com/read/26953552/silencing-of-usp22-suppresses-high-glucose-induced-apoptosis-ros-production-and-inflammation-in-podocytes
#9
Jian-Xia Shi, Qi-Jin Wang, Hui Li, Qin Huang
Ubiquitin-specific protease 22 (USP22) has been reported to mediate various cellular processes, including cell proliferation and apoptosis. However, its role in high glucose-induced podocytes and diabetic rats remains unknown. In the current study, podocytes were treated with different concentrations of d-glucose to establish a high glucose-induced injury model. Additionally, intravenous tail injection of rats with 65 mg kg(-1) of streptozotocin (STZ) was performed to establish a diabetic rat model. Our findings showed that the treatment of podocytes with high d-glucose significantly increased the USP22 expression level...
April 26, 2016: Molecular BioSystems
https://www.readbyqxmd.com/read/26559831/the-cellular-protein-complex-associated-with-a-transforming-region-of-e1a-contains-c-myc
#10
S Vijayalingam, T Subramanian, Ling-Jun Zhao, G Chinnadurai
UNLABELLED: The cell-transforming activity of human adenovirus 5 (hAd5) E1A is mediated by the N-terminal half of E1A, which interacts with three different major cellular protein complexes, p300/CBP, TRRAP/p400, and pRb family members. Among these protein interactions, the interaction of pRb family proteins with conserved region 2 (CR2) of E1A is known to promote cell proliferation by deregulating the activities of E2F family transcription factors. The functional consequences of interaction with the other two protein complexes in regulating the transforming activity of E1A are not well defined...
November 11, 2015: Journal of Virology
https://www.readbyqxmd.com/read/26195632/aggregation-of-polyglutamine-expanded-ataxin-7-protein-specifically-sequesters-ubiquitin-specific-protease-22-and-deteriorates-its-deubiquitinating-function-in-the-spt-ada-gcn5-acetyltransferase-saga-complex
#11
Hui Yang, Shuai Liu, Wen-Tian He, Jian Zhao, Lei-Lei Jiang, Hong-Yu Hu
Human ataxin 7 (Atx7) is a component of the deubiquitination module (DUBm) in the Spt-Ada-Gcn5-acetyltransferase (SAGA) complex for transcriptional regulation, and expansion of its polyglutamine (polyQ) tract leads to spinocerebellar ataxia type 7. However, how polyQ expansion of Atx7 affects DUBm function remains elusive. We investigated the effects of polyQ-expanded Atx7 on ubiquitin-specific protease (USP22), an interacting partner of Atx7 functioning in deubiquitination of histone H2B. The results showed that the inclusions or aggregates formed by polyQ-expanded Atx7 specifically sequester USP22 through their interactions mediated by the N-terminal zinc finger domain of Atx7...
September 4, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26171149/p38-mitogen-activated-protein-kinase-inhibits-usp22-transcription-in-hela-cells
#12
Jianjun Xiong, Zhen Gong, Xiaou Zhou, Jianyun Liu, H E Jiang, Ping Wu, Weidong Li
Elevated expression of ubiquitin-specific processing enzyme 22 (USP22) was identified in multiple types of human cancers, and was correlated with tumorigenesis and progression. Despite an increase in the numbers of studies in the physiological function of USP22, little is known regarding the regulation of its expression. The 5' flanking sequence of the USP22 gene was recently characterized. In the present study, USP22 transcription was regulated by p38 mitogen-activated protein kinase (MAPK). Treatment of human cervical carcinoma (HeLa) cells with SB203580, an inhibitor of p38 MAPK, enhanced basal USP22 promoter activity and mRNA abundance...
July 2015: Biomedical Reports
https://www.readbyqxmd.com/read/26143114/the-deubiquitinating-enzyme-activity-of-usp22-is-necessary-for-regulating-hela-cell-growth
#13
Ying-Li Liu, Jie Zheng, Li-Juan Tang, Wei Han, Jian-Min Wang, Dian-Wu Liu, Qing-Bao Tian
Ubiquitin-specific protease 22 (USP22) can regulate the cell cycle and apoptosis in many cancer cell types, while it is still unclear whether the deubiquitinating enzyme activity of USP22 is necessary for these processes. As little is known about the impact of USP22 on the growth of HeLa cell, we observed whether USP22 can effectively regulate HeLa cell growth as well as the necessity of deubiquitinating enzyme activity for these processes in HeLa cell. In this study, we demonstrate that USP22 can regulate cell cycle but not apoptosis in HeLa cell...
November 1, 2015: Gene
https://www.readbyqxmd.com/read/25973848/differential-genes-expression-between-fertile-and-infertile-spermatozoa-revealed-by-transcriptome-analysis
#14
Sandeep Kumar Bansal, Nishi Gupta, Satya Narayan Sankhwar, Singh Rajender
BACKGROUND: It was believed earlier that spermatozoa have no traces of RNA because of loss of most of the cytoplasm. Recent studies have revealed the presence of about 3000 different kinds of mRNAs in ejaculated spermatozoa. However, the correlation of transcriptome profile with infertility remains obscure. METHODS: Total RNA from sperm (after exclusion of somatic cells) of 60 men consisting of individuals with known fertility (n=20), idiopathic infertility (normozoospermic patients, n=20), and asthenozoospermia (n=20) was isolated...
2015: PloS One
https://www.readbyqxmd.com/read/25971547/decreased-h2b-monoubiquitination-and-overexpression-of-ubiquitin-specific-protease-enzyme-22-in-malignant-colon-carcinoma
#15
COMPARATIVE STUDY
Zijing Wang, Linlin Zhu, Tianjiao Guo, Yiping Wang, Jinlin Yang
This study aimed to evaluate the expression of H2B monoubiquitination enzyme (uH2B) and ubiquitin-specific protease enzyme 22 (USP22) in colon carcinoma and establish a correlation between the expression of these enzymes and clinicopathological parameters. The modification levels of uH2B and USP22 in 20 noncancerous and 129 cancerous colon samples were studied by immunohistochemistry. We used a dual-rated semiquantitative method to classify the expression according to 3 levels and analyzed these results. uH2B was abundant in the normal colon epithelium, but its expression was decreased in colon cancers (P < ...
July 2015: Human Pathology
https://www.readbyqxmd.com/read/25909224/high-usp22-expression-indicates-poor-prognosis-in-hepatocellular-carcinoma
#16
Bo Tang, Fang Tang, Bo Li, Shengguang Yuan, Qing Xu, Stephen Tomlinson, Junfei Jin, Wei Hu, Songqing He
Ubiquitin-specific protease 22 (USP22) removes ubiquitin from histones, thus regulating gene transcription. The expression frequency and expression levels of USP22 were significantly higher in hepatocellular carcinoma (HCC) than in normal liver tissues. High USP22 expression in HCC was significantly correlated with clinical stage and tumor grade. Kaplan-Meier analysis showed that elevated USP22 expression predicted poorer overall survival and recurrence-free survival. High USP22 expression was also associated with shortened survival time in patients at advanced tumor stages and with high grade HCC...
May 20, 2015: Oncotarget
https://www.readbyqxmd.com/read/25907317/usp22-promotes-tumor-progression-and-induces-epithelial-mesenchymal-transition-in-lung-adenocarcinoma
#17
Jing Hu, Dongdong Yang, Huijuan Zhang, Wei Liu, Yanbin Zhao, Hailing Lu, Qingwei Meng, Hui Pang, Xuesong Chen, Yanlong Liu, Li Cai
OBJECTIVES: Our previous study showed that USP22 as an oncogene may mediate cancer development and progression in NSCLC, but the underlying molecular mechanism remains uncharacterized. Epithelial-mesenchymal transition (EMT) has been reported to play an important role in migration and invasion of the tumor cells. Thus, this study aims to determine the clinical significance and the possible roles of USP22 in EMT and progression of lung adenocarcinoma. METHODS: Immunohistochemistry was used to determine the expression of USP22 in clinical samples...
June 2015: Lung Cancer: Journal of the International Association for the Study of Lung Cancer
https://www.readbyqxmd.com/read/25902005/ubiquitin-specific-peptidase-22-inhibits-colon-cancer-cell-invasion-by-suppressing-the-signal-transducer-and-activator-of-transcription-3-matrix-metalloproteinase-9-pathway
#18
Ning Ao, Yanyan Liu, Xiaocui Bian, Hailiang Feng, Yuqin Liu
Colon cancer is associated with increased cell migration and invasion. In the present study, the role of ubiquitin-specific peptidase 22 (USP22) in signal transducer and activator of transcription 3 (STAT3)-mediated colon cancer cell invasion was investigated. The messenger RNA levels of STAT3 target genes were measured by reverse transcription-quantitative polymerase chain reaction, following USP22 knockdown by RNA interference in SW480 colon cancer cells. The matrix metalloproteinase 9 (MMP9) proteolytic activity and invasion potential of SW480 cells were measured by zymography and Transwell assay, respectively, following combined USP22 and STAT3 short interfering (si)RNA treatment or STAT3 siRNA treatment alone...
August 2015: Molecular Medicine Reports
https://www.readbyqxmd.com/read/25846153/deubiquitinating-enzymes-regulate-hes1-stability-and-neuronal-differentiation
#19
Taeko Kobayashi, Yumiko Iwamoto, Kazuhiro Takashima, Akihiro Isomura, Yoichi Kosodo, Koichi Kawakami, Tomoki Nishioka, Kozo Kaibuchi, Ryoichiro Kageyama
Hairy and enhancer of split 1 (Hes1), a basic helix-loop-helix transcriptional repressor protein, regulates the maintenance of neural stem/progenitor cells by repressing proneural gene expression via Notch signaling. Previous studies showed that Hes1 expression oscillates in both mouse embryonic stem cells and neural stem cells, and that the oscillation contributes to their potency and differentiation fates. This oscillatory expression depends on the stability of Hes1, which is rapidly degraded by the ubiquitin/proteasome pathway...
July 2015: FEBS Journal
https://www.readbyqxmd.com/read/25817787/usp22-acts-as-an-oncogene-by-regulating-the-stability-of%C3%A2-cyclooxygenase-2-in-non-small-cell-lung-cancer
#20
Haibo Xiao, Yue Tian, Yang Yang, Fengqing Hu, Xiao Xie, Ju Mei, Fangbao Ding
The histone ubiquitin hydrolase ubiquitin-specific protease 22 (USP22) is an epigenetic modifier and an oncogene that is upregulated in many types of cancer. In non-small cell lung cancer (NSCLC), aberrant expression of USP22 is a predictor of poor survival, as is high expression of cyclooxygenase-2 (COX-2). Despite its oncogenic role, few substrates of USP22 have been identified and its mechanism of action in cancer remains unclear. Here, we identified COX-2 as a direct substrate of USP22 and showed that its levels are modulated by USP22 mediated deubiquitination...
May 8, 2015: Biochemical and Biophysical Research Communications
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